CSPI07G18440 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G18440
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Protein NIM1-INTERACTING 1
Location	Chr7: 16478748 .. 16479498 (-)
RNA-Seq Expression	CSPI07G18440
Synteny	CSPI07G18440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATAACCCCGCCGCTATGTGGAGCCTATTCTCGTCTTTCACCTTCAACCTCTATCACCTTATAAACAACGCCACTCCCATGCGAATTTTTTATTTTTTATTTTTAAAAAGAAAACAAAATTAGAGACGCAAACACCGCGTGGAAACCTTTTCCATTGTTTAATTATGGAAAACGGGAAACTACAGCCGTCGCCGGACACAACCGCGGAGGACTCGTTGGAGGATGAGGAGATGGAGAGGTTTTTCGCTTTGGTGAGGAGTTTGAAGGAAATGCGTGATCAGCGGAGGAAGGAGTTGAATATCGGACAAGAGGAGGGGGAGGAGGAAATCGCCGCGGGGGAGAAGAGGAGGAAGAGGATGAGGGCGGCGGAAATGGTGGAGAATCGGTCTACTTGGATTCCCAAGTTTGAGAGAGAGGATTTTGATGAGGAATTTCAGGCTTTGAGTACTCTTCCCCCCAACCCTTGTTGTAATCTTGTTAAAAGAGATACCACGGCGGCATCGACGACAACAACGGCGCCGATGAAGAATAAAAAGAAAGTGAAAAATGGGGACGTTTCTTTGGATCTTAATCTCGCTCTCTAATTTTTTTTCTTTTTTTTCCAATCTGAATCTCGACAACAACAAAAGTCGAATAGAATGAATAGATCATCAGAGTTTACAGTTTCAATCAAAATTTTGTTTTTGTTAATTTTGTTTAATCAACCAACCTAAACATATTATAATTATAAATTACTTACTGAATTACGAC

mRNA sequence

ATATAACCCCGCCGCTATGTGGAGCCTATTCTCGTCTTTCACCTTCAACCTCTATCACCTTATAAACAACGCCACTCCCATGCGAATTTTTTATTTTTTATTTTTAAAAAGAAAACAAAATTAGAGACGCAAACACCGCGTGGAAACCTTTTCCATTGTTTAATTATGGAAAACGGGAAACTACAGCCGTCGCCGGACACAACCGCGGAGGACTCGTTGGAGGATGAGGAGATGGAGAGGTTTTTCGCTTTGGTGAGGAGTTTGAAGGAAATGCGTGATCAGCGGAGGAAGGAGTTGAATATCGGACAAGAGGAGGGGGAGGAGGAAATCGCCGCGGGGGAGAAGAGGAGGAAGAGGATGAGGGCGGCGGAAATGGTGGAGAATCGGTCTACTTGGATTCCCAAGTTTGAGAGAGAGGATTTTGATGAGGAATTTCAGGCTTTGAGTACTCTTCCCCCCAACCCTTGTTGTAATCTTGTTAAAAGAGATACCACGGCGGCATCGACGACAACAACGGCGCCGATGAAGAATAAAAAGAAAGTGAAAAATGGGGACGTTTCTTTGGATCTTAATCTCGCTCTCTAATTTTTTTTCTTTTTTTTCCAATCTGAATCTCGACAACAACAAAAGTCGAATAGAATGAATAGATCATCAGAGTTTACAGTTTCAATCAAAATTTTGTTTTTGTTAATTTTGTTTAATCAACCAACCTAAACATATTATAATTATAAATTACTTACTGAATTACGAC

Coding sequence (CDS)

ATGGAAAACGGGAAACTACAGCCGTCGCCGGACACAACCGCGGAGGACTCGTTGGAGGATGAGGAGATGGAGAGGTTTTTCGCTTTGGTGAGGAGTTTGAAGGAAATGCGTGATCAGCGGAGGAAGGAGTTGAATATCGGACAAGAGGAGGGGGAGGAGGAAATCGCCGCGGGGGAGAAGAGGAGGAAGAGGATGAGGGCGGCGGAAATGGTGGAGAATCGGTCTACTTGGATTCCCAAGTTTGAGAGAGAGGATTTTGATGAGGAATTTCAGGCTTTGAGTACTCTTCCCCCCAACCCTTGTTGTAATCTTGTTAAAAGAGATACCACGGCGGCATCGACGACAACAACGGCGCCGATGAAGAATAAAAAGAAAGTGAAAAATGGGGACGTTTCTTTGGATCTTAATCTCGCTCTCTAA

Protein sequence

MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEKRRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAASTTTTAPMKNKKKVKNGDVSLDLNLAL*

Homology

[Close]

BLAST of CSPI07G18440 vs. ExPASy TrEMBL
Match: A0A0A0K5V7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414420 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.7e-68
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60
           MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK
Sbjct: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60

Query: 61  RRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAASTTTTAPM 120
           RRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAASTTTTAPM
Sbjct: 61  RRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAASTTTTAPM 120

Query: 121 KNKKKVKNGDVSLDLNLAL 140
           KNKKKVKNGDVSLDLNLAL
Sbjct: 121 KNKKKVKNGDVSLDLNLAL 139

[Close]

BLAST of CSPI07G18440 vs. ExPASy TrEMBL
Match: A0A5A7UYI4 (Protein NIM1-INTERACTING 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00070 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 1.0e-43
Identity = 105/141 (74.47%), Postives = 116/141 (82.27%), Query Frame = 0


Query: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60
           MEN K+QP P+ TAE+ LE+EEMERFFALVRS KEMRDQR KELN+G+EE        EK
Sbjct: 1   MENEKMQPLPERTAEELLEEEEMERFFALVRSFKEMRDQRWKELNMGEEE-------EEK 60

Query: 61  RRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAA--STTTTA 120
           RRKRM+A +MVENRSTWIPKFEREDFDEEFQALS   P PCCNL KR T AA  +TTTTA
Sbjct: 61  RRKRMKAGKMVENRSTWIPKFEREDFDEEFQALSL--PEPCCNLDKRGTRAATTTTTTTA 120

Query: 121 PMKNKKKVKNGDVSLDLNLAL 140
            MKNKK+VKNG+VSLDLNLAL
Sbjct: 121 AMKNKKRVKNGEVSLDLNLAL 132

[Close]

BLAST of CSPI07G18440 vs. ExPASy TrEMBL
Match: A0A6J1JVD5 (uncharacterized protein LOC111488189 OS=Cucurbita maxima OX=3661 GN=LOC111488189 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.1e-21
Identity = 73/141 (51.77%), Postives = 95/141 (67.38%), Query Frame = 0


Query: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60
           MEN K QP  +T AE+  E+ EME+F+ALVR+ KE+RD+ RKE+  G  EG+E   A EK
Sbjct: 1   MENQKRQPPRETIAEEEEEEVEMEKFYALVRNFKEIRDRWRKEVVNG--EGDE--TAAEK 60

Query: 61  RRKRMRAAEMVENRSTWIPKFEREDFDEEFQ--ALSTLPPNPCCNLVKRDTTAASTTTTA 120
            RKRM+  E V+N+S+WI +FEREDFDE+ Q    +   P  CC LVK DT  A+    A
Sbjct: 61  TRKRMKTTETVQNQSSWILRFEREDFDEDLQVGGAAVELPKHCC-LVKEDTPVAA----A 120

Query: 121 PMKNKKKVKNGDVSLDLNLAL 140
           P+K +KKV +G   LDLNL+L
Sbjct: 121 PLKKEKKVASGVDCLDLNLSL 132

[Close]

BLAST of CSPI07G18440 vs. ExPASy TrEMBL
Match: A0A6J1H831 (protein NIM1-INTERACTING 1 OS=Cucurbita moschata OX=3662 GN=LOC111460992 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.9e-17
Identity = 68/128 (53.12%), Postives = 81/128 (63.28%), Query Frame = 0


Query: 14  AEDSLEDEE--MERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEKRRKRMRAAEMV 73
           A D  E+EE  ME FFALVR+ KEMRDQRRKEL IG +   E+ AA E R+++M+    V
Sbjct: 21  AVDGEEEEEMKMEAFFALVRNFKEMRDQRRKELMIGCD--GEKAAAAEMRKRKMKT---V 80

Query: 74  ENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAASTTTTAPMKNKKKVKNGDV 133
           ENRS WIP+FEREDFDEEFQ              K +  AA+  T    K KKKVKN + 
Sbjct: 81  ENRSAWIPRFEREDFDEEFQVRGP---------AKEEAAAAAIATA---KKKKKVKNEED 131

Query: 134 SLDLNLAL 140
            LDLNL+L
Sbjct: 141 ILDLNLSL 131

[Close]

BLAST of CSPI07G18440 vs. ExPASy TrEMBL
Match: A0A6J1DAZ5 (protein NIM1-INTERACTING 1 OS=Momordica charantia OX=3673 GN=LOC111019022 PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 4.1e-16
Identity = 68/141 (48.23%), Postives = 85/141 (60.28%), Query Frame = 0


Query: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60
           MEN  L+P+    AE+  E+ +ME FFAL+R+ KEMRD+RRKEL     +G E     EK
Sbjct: 1   MENENLRPA---AAEEEEEEMKMEAFFALLRNFKEMRDRRRKELTTMIGDGGET----EK 60

Query: 61  RRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLP--PNPCCNLVKRDTTAASTTTTA 120
           +RK+M+ A      STWIP F REDFDE+FQ     P  P P CNL+K DT        A
Sbjct: 61  KRKKMKTAP-----STWIPTFLREDFDEDFQVRGPPPVLPKP-CNLIKEDT--PPPPLPA 120

Query: 121 PMKNKKKVKNGDVSLDLNLAL 140
            +  KK +KN +  LDLNLAL
Sbjct: 121 AVAKKKNMKNDEECLDLNLAL 126

[Close]

BLAST of CSPI07G18440 vs. NCBI nr
Match: KGN45045.1 (hypothetical protein Csa_015768 [Cucumis sativus])

HSP 1 Score: 267.7 bits (683), Expect = 5.7e-68
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60
           MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK
Sbjct: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60

Query: 61  RRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAASTTTTAPM 120
           RRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAASTTTTAPM
Sbjct: 61  RRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAASTTTTAPM 120

Query: 121 KNKKKVKNGDVSLDLNLAL 140
           KNKKKVKNGDVSLDLNLAL
Sbjct: 121 KNKKKVKNGDVSLDLNLAL 139

[Close]

BLAST of CSPI07G18440 vs. NCBI nr
Match: KAA0058541.1 (protein NIM1-INTERACTING 1 [Cucumis melo var. makuwa] >TYJ96897.1 protein NIM1-INTERACTING 1 [Cucumis melo var. makuwa])

HSP 1 Score: 186.0 bits (471), Expect = 2.2e-43
Identity = 105/141 (74.47%), Postives = 116/141 (82.27%), Query Frame = 0


Query: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60
           MEN K+QP P+ TAE+ LE+EEMERFFALVRS KEMRDQR KELN+G+EE        EK
Sbjct: 1   MENEKMQPLPERTAEELLEEEEMERFFALVRSFKEMRDQRWKELNMGEEE-------EEK 60

Query: 61  RRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLPPNPCCNLVKRDTTAA--STTTTA 120
           RRKRM+A +MVENRSTWIPKFEREDFDEEFQALS   P PCCNL KR T AA  +TTTTA
Sbjct: 61  RRKRMKAGKMVENRSTWIPKFEREDFDEEFQALSL--PEPCCNLDKRGTRAATTTTTTTA 120

Query: 121 PMKNKKKVKNGDVSLDLNLAL 140
            MKNKK+VKNG+VSLDLNLAL
Sbjct: 121 AMKNKKRVKNGEVSLDLNLAL 132

[Close]

BLAST of CSPI07G18440 vs. NCBI nr
Match: XP_038898983.1 (protein NIM1-INTERACTING 1-like [Benincasa hispida])

HSP 1 Score: 157.1 bits (396), Expect = 1.1e-34
Identity = 97/142 (68.31%), Postives = 110/142 (77.46%), Query Frame = 0


Query: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60
           MEN KLQP+P+  AE+  E+EEME FFALVR+ KEMRD+RRKEL IG+   EEEIAA EK
Sbjct: 1   MENEKLQPAPERIAEEE-EEEEMEMFFALVRNFKEMRDRRRKELMIGE---EEEIAAEEK 60

Query: 61  RRKRMRAA-EMVENRSTWIPKFEREDFDEEFQALST--LPPNPCCNLVKRDTTAASTTTT 120
           RRKRM++A E VENRSTWIPKFEREDFDEEFQ      + PN   NLVK DT+AA+ +T 
Sbjct: 61  RRKRMKSAPEAVENRSTWIPKFEREDFDEEFQVRGQGFILPNKPYNLVKEDTSAAAASTA 120

Query: 121 APMKNKKKVKNGDVSLDLNLAL 140
           A MK KK VKNGD  LDLNLAL
Sbjct: 121 A-MKKKKNVKNGDDCLDLNLAL 137

[Close]

BLAST of CSPI07G18440 vs. NCBI nr
Match: XP_022991628.1 (uncharacterized protein LOC111488189 [Cucurbita maxima] >XP_022991629.1 uncharacterized protein LOC111488189 [Cucurbita maxima] >XP_022991630.1 uncharacterized protein LOC111488189 [Cucurbita maxima])

HSP 1 Score: 112.8 bits (281), Expect = 2.3e-21
Identity = 73/141 (51.77%), Postives = 95/141 (67.38%), Query Frame = 0


Query: 1   MENGKLQPSPDTTAEDSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIAAGEK 60
           MEN K QP  +T AE+  E+ EME+F+ALVR+ KE+RD+ RKE+  G  EG+E   A EK
Sbjct: 1   MENQKRQPPRETIAEEEEEEVEMEKFYALVRNFKEIRDRWRKEVVNG--EGDE--TAAEK 60

Query: 61  RRKRMRAAEMVENRSTWIPKFEREDFDEEFQ--ALSTLPPNPCCNLVKRDTTAASTTTTA 120
            RKRM+  E V+N+S+WI +FEREDFDE+ Q    +   P  CC LVK DT  A+    A
Sbjct: 61  TRKRMKTTETVQNQSSWILRFEREDFDEDLQVGGAAVELPKHCC-LVKEDTPVAA----A 120

Query: 121 PMKNKKKVKNGDVSLDLNLAL 140
           P+K +KKV +G   LDLNL+L
Sbjct: 121 PLKKEKKVASGVDCLDLNLSL 132

[Close]

BLAST of CSPI07G18440 vs. NCBI nr
Match: XP_023547822.1 (uncharacterized protein LOC111806674 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 99.4 bits (246), Expect = 2.7e-17
Identity = 71/145 (48.97%), Postives = 91/145 (62.76%), Query Frame = 0


Query: 1   MENGKLQPSPDTTAE----DSLEDEEMERFFALVRSLKEMRDQRRKELNIGQEEGEEEIA 60
           MEN K QP   T AE    ++ E+ EME F+ALVR+ KE+RD+ RKE+ +  E GE    
Sbjct: 1   MENQKRQPPRGTIAEEEEAEAEEEVEMETFYALVRNFKEIRDRCRKEV-VNGEGGE---T 60

Query: 61  AGEKRRKRMRAAEMVENRSTWIPKFEREDFDEEFQALSTLP--PNPCCNLVKRDTTAAST 120
           A EK RKRM+  E  +N+S+WI +FEREDFDE+ Q   T    P  CC LVK DT  A+ 
Sbjct: 61  AAEKTRKRMKTTETSQNQSSWILRFEREDFDEDLQVGGTAVELPKRCC-LVKEDTPVAAP 120

Query: 121 TTTAPMKNKKKVKNGDVSLDLNLAL 140
            +   MK +KKV +G   LDLNL+L
Sbjct: 121 PS---MKKEKKVASGVDCLDLNLSL 137

The following BLAST results are available for this feature:

BLAST of CSPI07G18440 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI07G18440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K5V7	2.7e-68	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414420 PE=3 SV=1	[more]
A0A5A7UYI4	1.0e-43	74.47	Protein NIM1-INTERACTING 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A6J1JVD5	1.1e-21	51.77	uncharacterized protein LOC111488189 OS=Cucurbita maxima OX=3661 GN=LOC111488189...	[more]
A0A6J1H831	4.9e-17	53.13	protein NIM1-INTERACTING 1 OS=Cucurbita moschata OX=3662 GN=LOC111460992 PE=3 SV...	[more]
A0A6J1DAZ5	4.1e-16	48.23	protein NIM1-INTERACTING 1 OS=Momordica charantia OX=3673 GN=LOC111019022 PE=3 S...	[more]

BLAST of CSPI07G18440 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN45045.1	5.7e-68	100.00	hypothetical protein Csa_015768 [Cucumis sativus]	[more]
KAA0058541.1	2.2e-43	74.47	protein NIM1-INTERACTING 1 [Cucumis melo var. makuwa] >TYJ96897.1 protein NIM1-I...	[more]
XP_038898983.1	1.1e-34	68.31	protein NIM1-INTERACTING 1-like [Benincasa hispida]	[more]
XP_022991628.1	2.3e-21	51.77	uncharacterized protein LOC111488189 [Cucurbita maxima] >XP_022991629.1 uncharac...	[more]
XP_023547822.1	2.7e-17	48.97	uncharacterized protein LOC111806674 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI07G18440 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031425	NPR1/NH1-interacting protein	PFAM	PF15699	NPR1_interact	coord: 15..136 e-value: 1.1E-18 score: 67.8
IPR031425	NPR1/NH1-interacting protein	PANTHER	PTHR33669	PROTEIN NEGATIVE REGULATOR OF RESISTANCE	coord: 3..139
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR33669:SF4	PROTEIN NIM1-INTERACTING 3	coord: 3..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G18440.1	CSPI07G18440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010112	regulation of systemic acquired resistance
cellular_component	GO:0005634	nucleus