CSPI07G07530 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G07530
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDiacylglycerol O-acyltransferase
LocationChr7: 5380711 .. 5383978 (-)
RNA-Seq ExpressionCSPI07G07530
SyntenyCSPI07G07530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCTTGAGAAATAACTATATATATATTTTTTGTAAATGTGGTAGGAAAAAAGTTGAAATTTAATACAACTAAAAATATACAATATATAAAAGAGGACCACGTCTCTTCTCTCTCTATAACTTTCCTTTCTTTGCCTCTCTGCTACACTCTTCGACTTCTCCACTCTTCTGTCTTTGACCTCTGCTTATTAGCCATTTTCATGGAACGTCCATTTCATCTTCACCCTACTCTTCTCTCTCTCTTTCCTTATACCACCTAATCTCATCATTCCTTCAATACCCTTTTCCCCTCTCCATGAATTCCGACGAGCCACTCACTCCTGCCGGCCGTCTCTTCCTCCGTCCTGAAATCAACCAGATTATCCACTGTCTCGTTGGACTAAAGAACTCAATCGATGTCGATTCTGTCAAATCTCAAATCGCTGATTCCATCATGATTCAGCACCCTAGGTTTTCGAGTCTTCTCGTTCGTGACCGCAACGGCGTTGAGTATTGGCGGAGGACTTCAATCGAAGTCGACCGTCACGTCATCGTCGTTTCCGATCCTGTATCGGATGACGTTGGAGGAGTAAACGATGAGAAGGCTGCGAATGAATATTTGGCTGATTTAGCGATTTCTTCCTCCATGGATTACAGCAAACCTCTTTGGGAGATTCATTTGCTTTTGGCGCATAACTGTGCCGTCTTCCGAATCCATCACGCGCTCGGTGATGGAATCTCGTTGATGTCGTTGTTCTTGACGTGTTGCCGGCGGGCTGATGATCCGGATGCGTTGCCGACGATCGTTTCGGATTTGAAGGCGGTGAGGACTGGAAACAGAGGGCGGAGGAGTTGTGGGGAGATGATGTTGGAGTTTCTTTTAACGGTGTGGTTTAGTTTGTTGTTTGTGTTGGAGTTTATCGTGAGAGCTTTGTGGGTTTGTGACCGGAAAACGCCGATATCCGGTGGGGATGGGGTGGAGCTTTGGCCGAGGAAAGTGGCGACGGCTAAGTTCGCGTTGGAGGATATGAAAGCGGTGAAGAAAGGAGTTCCTAACGCGGTAAGTTGGACTTCTTAGCGTCACCTTTTCCCCAAACTAAATTTATTCATTTTCGTACTCTAAAATAGAATTTTCTCTCGGAAATAGATATATATCCAAAAGCAATACAATTTTAACTAAAATGTTCAAAGGTAAACATTCATAAATAATAATGGAACCAACATTTCCCATAACTATAACATATATAATTATTTCCCCAGGGGGACCTATGTTCAAAATGAAGAAAGAAATAGATAAAAAAGAGGCAATCCTTTTTTTCTTTTCTTTCTTATGTAATTTTTGTTCTTACTATTTTCTTTTAGCAAAATTTGTTTTGTTACAAAAAGGAGGGAGAACGTGTTGAAGTCATCAAAACCAAAGACATGTTTAGAAATAAGCTTTTTTTCTTCTATCCAAAAATAATTTTGGTTAGTTTCTAAATAAATGTGTGTAGCATTACAAAAGAATTATTGAAGAATTTTATTTAAAAATGTAGAACGTAGCTAATTGTGATTGGATCAAACATTTATGATTGGTTGCAGACCATAAATGACGTTCTATTCAGCGTAATAGGAGCAGGGTTATCGAGGTACTTGGAGCATAGGCAACCCAAAGGTTAGTCTTTCCTTTACCACTCCCAACTATTTTATACTCCAATGTATTTTGTTTGGTTAAATAACATTTTTTGCTCTTCAAATTTTAATATTGTTCTTGCTTTTAAAGTTTTATGTCTAATAAATTAGATGGGCCTATTACTTACTTTTTGAAGTTAGGTTTAGTTGGGAGCATCAAATTAATATTTCATTGACTTATTGACCCAACTTTAATTTAAATTTTTAGGACTTCTTTATCGCTTTGTAAACATTCTTTTTACTTTTAGGTTTGAAAGAAGGACTTCAATTAACTGGAGTAGCTATGGTTAATCTTAGAGAGCAACCGGGATTGCAGGTTTTATAGATTATTATTTTTAAATAAAAATTTTCTTTTTGTTAAGAATTTTCTATTATTATACTACATTGTCGTCAACTCTCTATATGTATGGTATAGGATTTATCTGATATGATGAAAGGAAACAAGGGATCACGATGGGGTAACAAGTTAGGAATTCTTCTCCTTCCTGTAAACTATTACACGAAAGCACTCGATCCTCTTCAATATGTCAAGAGAACGAAAAAGATGCTTGATCGGAAAAAACGGACCTTCGAAGCTCATTTTTCGTACGGAATTGGCAAGTTGGTAATGTCCTTTTTGGGACCAAAGGTAGGATTATAGAAATTTAAAAGGTTGTATAGTTCTAAATTTCTCATTATTGTTTTTAGATGTTTAGTAAATTATTTGTACACAATGTGTTTGCAGGTAGCTTGCATCCTTAATTACAGGATTGTTTGCAATACAAGTTTCACTATCTCCAATGTGATTGGCCCAAGAGAAGAGATTACCATTGGAGGCAATCCCGTTACTTACATCAGAGTCACTTCAACAAGCTTATCCCATGTACTACCACCTCCCACCTCTCTTCTTACCTATTTCATAACAAAACTTCAAAATGATTTTATGGACAGATCTAATAGAAATATATTGGTTAGTAGAGAAACATTATGTAATAGAAACGAATATGGTTTGATGCAGGCATTAACAATGCATATGATGAGCTATGCTGGAAGAGCTGAAATGCAAATTTTGGTGGCAAAAGACATCATCCCTGATCCTGAATTCCTTGCTGAATGCTTTGAAAATGCTTTACTTGAAATGAAAACAGCAGGAGCAACATTAACCACAAAATAGCATATACAATAACTTCCCAAGTTTAATTTTCTACTTGTTTATACATTGTTTAATCAAGAAAAAGCCAATCGATTAGACTTTATGAGGTAACGGTGTTTGAAACTACTGTTGAAATAGTTAAAATTATATTTTTTTCCATATAATTCTAAACTTTGAAATACGATTTTAATAAATCAAAATTGAGAATTATTTTGGGTGTGTGTTGGAATTATCTGAAAGAGGTATGCCATCAGTTTTGTAAGGACAATTGTCTCTACCAAAATAAGAAAGAAACAGGAAAACAAAGGACAGAAAGGGCATGGAGGTACAACTGTATAAACCACATATTTTGTCTACTTTAATTTCCTTTATGAAAAATGGAAAGAGGTCATAAATGATATTTGACTAAATAAATCGTATCAACTTTTACCATAGACTTTTAATTAGTGTAAATTACACTTTTACACTGTGATAAGCAATGCAAT

mRNA sequence

TTTTCTTGAGAAATAACTATATATATATTTTTTGTAAATGTGGTAGGAAAAAAGTTGAAATTTAATACAACTAAAAATATACAATATATAAAAGAGGACCACGTCTCTTCTCTCTCTATAACTTTCCTTTCTTTGCCTCTCTGCTACACTCTTCGACTTCTCCACTCTTCTGTCTTTGACCTCTGCTTATTAGCCATTTTCATGGAACGTCCATTTCATCTTCACCCTACTCTTCTCTCTCTCTTTCCTTATACCACCTAATCTCATCATTCCTTCAATACCCTTTTCCCCTCTCCATGAATTCCGACGAGCCACTCACTCCTGCCGGCCGTCTCTTCCTCCGTCCTGAAATCAACCAGATTATCCACTGTCTCGTTGGACTAAAGAACTCAATCGATGTCGATTCTGTCAAATCTCAAATCGCTGATTCCATCATGATTCAGCACCCTAGGTTTTCGAGTCTTCTCGTTCGTGACCGCAACGGCGTTGAGTATTGGCGGAGGACTTCAATCGAAGTCGACCGTCACGTCATCGTCGTTTCCGATCCTGTATCGGATGACGTTGGAGGAGTAAACGATGAGAAGGCTGCGAATGAATATTTGGCTGATTTAGCGATTTCTTCCTCCATGGATTACAGCAAACCTCTTTGGGAGATTCATTTGCTTTTGGCGCATAACTGTGCCGTCTTCCGAATCCATCACGCGCTCGGTGATGGAATCTCGTTGATGTCGTTGTTCTTGACGTGTTGCCGGCGGGCTGATGATCCGGATGCGTTGCCGACGATCGTTTCGGATTTGAAGGCGGTGAGGACTGGAAACAGAGGGCGGAGGAGTTGTGGGGAGATGATGTTGGAGTTTCTTTTAACGGTGTGGTTTAGTTTGTTGTTTGTGTTGGAGTTTATCGTGAGAGCTTTGTGGGTTTGTGACCGGAAAACGCCGATATCCGGTGGGGATGGGGTGGAGCTTTGGCCGAGGAAAGTGGCGACGGCTAAGTTCGCGTTGGAGGATATGAAAGCGGTGAAGAAAGGAGTTCCTAACGCGACCATAAATGACGTTCTATTCAGCGTAATAGGAGCAGGGTTATCGAGGTACTTGGAGCATAGGCAACCCAAAGGTTTGAAAGAAGGACTTCAATTAACTGGAGTAGCTATGGTTAATCTTAGAGAGCAACCGGGATTGCAGGATTTATCTGATATGATGAAAGGAAACAAGGGATCACGATGGGGTAACAAGTTAGGAATTCTTCTCCTTCCTGTAAACTATTACACGAAAGCACTCGATCCTCTTCAATATGTCAAGAGAACGAAAAAGATGCTTGATCGGAAAAAACGGACCTTCGAAGCTCATTTTTCGTACGGAATTGGCAAGTTGGTAATGTCCTTTTTGGGACCAAAGGTAGCTTGCATCCTTAATTACAGGATTGTTTGCAATACAAGTTTCACTATCTCCAATGTGATTGGCCCAAGAGAAGAGATTACCATTGGAGGCAATCCCGTTACTTACATCAGAGTCACTTCAACAAGCTTATCCCATGCATTAACAATGCATATGATGAGCTATGCTGGAAGAGCTGAAATGCAAATTTTGGTGGCAAAAGACATCATCCCTGATCCTGAATTCCTTGCTGAATGCTTTGAAAATGCTTTACTTGAAATGAAAACAGCAGGAGCAACATTAACCACAAAATAGCATATACAATAACTTCCCAAGTTTAATTTTCTACTTGTTTATACATTGTTTAATCAAGAAAAAGCCAATCGATTAGACTTTATGAGGTAACGGTGTTTGAAACTACTGTTGAAATAGTTAAAATTATATTTTTTTCCATATAATTCTAAACTTTGAAATACGATTTTAATAAATCAAAATTGAGAATTATTTTGGGTGTGTGTTGGAATTATCTGAAAGAGGTATGCCATCAGTTTTGTAAGGACAATTGTCTCTACCAAAATAAGAAAGAAACAGGAAAACAAAGGACAGAAAGGGCATGGAGGTACAACTGTATAAACCACATATTTTGTCTACTTTAATTTCCTTTATGAAAAATGGAAAGAGGTCATAAATGATATTTGACTAAATAAATCGTATCAACTTTTACCATAGACTTTTAATTAGTGTAAATTACACTTTTACACTGTGATAAGCAATGCAAT

Coding sequence (CDS)

ATGAATTCCGACGAGCCACTCACTCCTGCCGGCCGTCTCTTCCTCCGTCCTGAAATCAACCAGATTATCCACTGTCTCGTTGGACTAAAGAACTCAATCGATGTCGATTCTGTCAAATCTCAAATCGCTGATTCCATCATGATTCAGCACCCTAGGTTTTCGAGTCTTCTCGTTCGTGACCGCAACGGCGTTGAGTATTGGCGGAGGACTTCAATCGAAGTCGACCGTCACGTCATCGTCGTTTCCGATCCTGTATCGGATGACGTTGGAGGAGTAAACGATGAGAAGGCTGCGAATGAATATTTGGCTGATTTAGCGATTTCTTCCTCCATGGATTACAGCAAACCTCTTTGGGAGATTCATTTGCTTTTGGCGCATAACTGTGCCGTCTTCCGAATCCATCACGCGCTCGGTGATGGAATCTCGTTGATGTCGTTGTTCTTGACGTGTTGCCGGCGGGCTGATGATCCGGATGCGTTGCCGACGATCGTTTCGGATTTGAAGGCGGTGAGGACTGGAAACAGAGGGCGGAGGAGTTGTGGGGAGATGATGTTGGAGTTTCTTTTAACGGTGTGGTTTAGTTTGTTGTTTGTGTTGGAGTTTATCGTGAGAGCTTTGTGGGTTTGTGACCGGAAAACGCCGATATCCGGTGGGGATGGGGTGGAGCTTTGGCCGAGGAAAGTGGCGACGGCTAAGTTCGCGTTGGAGGATATGAAAGCGGTGAAGAAAGGAGTTCCTAACGCGACCATAAATGACGTTCTATTCAGCGTAATAGGAGCAGGGTTATCGAGGTACTTGGAGCATAGGCAACCCAAAGGTTTGAAAGAAGGACTTCAATTAACTGGAGTAGCTATGGTTAATCTTAGAGAGCAACCGGGATTGCAGGATTTATCTGATATGATGAAAGGAAACAAGGGATCACGATGGGGTAACAAGTTAGGAATTCTTCTCCTTCCTGTAAACTATTACACGAAAGCACTCGATCCTCTTCAATATGTCAAGAGAACGAAAAAGATGCTTGATCGGAAAAAACGGACCTTCGAAGCTCATTTTTCGTACGGAATTGGCAAGTTGGTAATGTCCTTTTTGGGACCAAAGGTAGCTTGCATCCTTAATTACAGGATTGTTTGCAATACAAGTTTCACTATCTCCAATGTGATTGGCCCAAGAGAAGAGATTACCATTGGAGGCAATCCCGTTACTTACATCAGAGTCACTTCAACAAGCTTATCCCATGCATTAACAATGCATATGATGAGCTATGCTGGAAGAGCTGAAATGCAAATTTTGGTGGCAAAAGACATCATCCCTGATCCTGAATTCCTTGCTGAATGCTTTGAAAATGCTTTACTTGAAATGAAAACAGCAGGAGCAACATTAACCACAAAATAG

Protein sequence

MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSCGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK*
Homology
BLAST of CSPI07G07530 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 3.7e-74
Identity = 175/484 (36.16%), Postives = 271/484 (55.99%), Query Frame = 0

Query: 4   DEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNG 63
           ++PL+PA RLF  PE N  I  +VGLKN I+ D +   I  ++M +HPRFSS LV + N 
Sbjct: 25  EQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVNNCNN 84

Query: 64  ---VEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 123
               + W RT++ V+ HVI+     +  +   N +     Y++DL  +  +D SKPLWE+
Sbjct: 85  NRQEQKWVRTNVVVEDHVIIPKIQ-TQHIENANADVFLESYVSDLT-TIPLDTSKPLWEV 144

Query: 124 HLL-----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTI------VSDLKA 183
           HLL      A N AV RIHH+LGDG+S+MSL L C R+  +P+ LP++       S   +
Sbjct: 145 HLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSGSSS 204

Query: 184 VRTGNRGRRSCGEMMLEFLLTVWFSLLFV-------LEFIVRALWVCDRKTPISGGDGVE 243
           ++T +R    C       ++ +W + L V       LEFI  AL++ D +TPI G   + 
Sbjct: 205 LKTSSR----CYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIKGDFKLS 264

Query: 244 LWPRKVATAK-FALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEH---------RQPK 303
              R     +  +L+D+K +K  +   T+NDV+  V  AGLS+YL+          R   
Sbjct: 265 KGKRMCMVHRTVSLDDIKLIKNAM-KMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNSS 324

Query: 304 GLKEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKAL--DPL 363
            + +G++L    +VNLR   G+QDL+DMM      RWGN +G ++ P   ++ AL  DPL
Sbjct: 325 NIPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFP---FSIALCDDPL 384

Query: 364 QYVKRTKKMLDRKKRTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTISNVIGPR 423
           ++++R K  +DRKK + EA  ++ +GK++++ LG + A  +  R + NT+ + SN++GP 
Sbjct: 385 KHLRRAKSTIDRKKNSLEAVLTFVVGKILLNTLGVQRAANVLNRALSNTTMSFSNLVGPV 444

Query: 424 EEITIGGNPVTYIRVTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLAECFENAL 455
           EEI+  G+ VTYI  +     HALTMH  SY  +  + + V   +I DP  L + +E +L
Sbjct: 445 EEISFYGHTVTYIAPSVYGHPHALTMHFQSYMNKLTISLTVDPTVISDPHKLCDDWEESL 497

BLAST of CSPI07G07530 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.2e-72
Identity = 168/472 (35.59%), Postives = 254/472 (53.81%), Query Frame = 0

Query: 4   DEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNG 63
           +EPL+P  RLF  P+ N  I   +G K   D  ++   + +  ++ HPRFSS+L  +   
Sbjct: 14  EEPLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGL-EHTLVNHPRFSSILEMNNGK 73

Query: 64  VEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHLL 123
              W RT ++V+ HVIV    V  D+   N ++   +Y++ L  +  MD SKPLWE+HLL
Sbjct: 74  KPRWVRTKVKVEEHVIV--PDVDPDI--ENPDQYLEDYISKLT-TIPMDLSKPLWEMHLL 133

Query: 124 -----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRR 183
                 A + A+ +IHH+LGDG+SLMSL L C R+  DP+ALPT+    K  R G     
Sbjct: 134 GVKTSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKK--RFGPSCNS 193

Query: 184 SCGEMMLEFLLTVWF--SLLF-----VLEFIVRALWVCDRKTPISGGDGVELWPRKVATA 243
                +    + +WF   LLF     +L F +    + D +TP+    G EL P++    
Sbjct: 194 GFFNKIWWLFVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLAKPGSELIPKRFVHR 253

Query: 244 KFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEHR-------QPKGLKEGLQLTGVA 303
             + +D+K VK  +   T+NDVL  V  AGLSRYL  +       + K     ++L    
Sbjct: 254 IISFDDVKLVKNAM-KMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAI 313

Query: 304 MVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKK 363
           M+NLR   G++ L+DMM      RWGN  G +LLP +   +  DPL+YV++ K  +DRKK
Sbjct: 314 MINLRPNAGIEALADMMAKKSKCRWGNLFGYILLPFSVGLET-DPLEYVRQAKATIDRKK 373

Query: 364 RTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIR 423
            + EA FS    KL++  LG K + +L  +++ +T+ + SNV+GP+EEIT  G+P+ YI 
Sbjct: 374 HSLEAVFSMAFFKLILKVLGLKASVVLVRKVIHSTTLSFSNVVGPKEEITFHGHPLNYIS 433

Query: 424 VTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTA 457
                  HALT+H  +YA +  + +     +IPDP  + +    +L  +K A
Sbjct: 434 PCVFGHPHALTLHFQTYANKVIISVTADPTVIPDPHKMCDDLVESLKMIKAA 475

BLAST of CSPI07G07530 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 4.5e-72
Identity = 170/482 (35.27%), Postives = 267/482 (55.39%), Query Frame = 0

Query: 4   DEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNG 63
           ++PL+PA RLF  PE N  I  ++G K+ +D         +S  I+HPRFSS LV D NG
Sbjct: 17  EQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKES-FIRHPRFSSKLVTDENG 76

Query: 64  V-EYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHL 123
             + W RT++ V+ H IV   P        N      +Y++DL +   +D S+PLWE+HL
Sbjct: 77  QNQRWVRTNVVVEDHFIV---PKIKPQNIENSNAFLEDYVSDL-MKIPLDTSRPLWELHL 136

Query: 124 L-----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKA------VR 183
           L      A N AV +IHH++GDG+S+MSL L C R+  +PD LP++    ++      + 
Sbjct: 137 LDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLT 196

Query: 184 TGNRGRRSCGEMMLEFLLTVWFSLLF-------VLEFIVRALWVCDRKTPISGGD-GVEL 243
           TG+R        +L  +  +W +++         LEFIV  L+V D +TPI G     + 
Sbjct: 197 TGSRS----DSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIKGDFLSTKS 256

Query: 244 WPRKVATAKFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYL---------EHRQPKGL 303
              ++     +L+D+K  K  + N TINDV+  V  AGLSRYL         ++R+ K L
Sbjct: 257 KQLRLVHRTVSLDDIKLTKNAM-NMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKL 316

Query: 304 KEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVK 363
            + ++L    +VNLR   G+QDL+DMM+     RWGN  G ++ P +   +  DPL++++
Sbjct: 317 PKRIRLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFPFSIALRD-DPLEHLE 376

Query: 364 RTKKMLDRKKRTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTISNVIGPREEIT 423
             +K + RKK ++ A  +Y   ++++  LG KVA  +  R+V NT+ T SN++GP EE++
Sbjct: 377 IAQKTISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEEVS 436

Query: 424 IGGNPVTYIRVTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMK 457
             G+P+TY   ++    HALT+H  SY  +  + ++V   +I DP  L + +E +L  +K
Sbjct: 437 FYGHPITYFASSAYGHPHALTIHCQSYMNKMTITLIVDPTVISDPHRLCDDWEESLRSIK 487

BLAST of CSPI07G07530 vs. ExPASy Swiss-Prot
Match: Q9M3B1 (Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=WSD6 PE=1 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.9e-71
Identity = 165/493 (33.47%), Postives = 269/493 (54.56%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           +  ++PL+PA R+F  PE N  +  ++G+K  ID D +   +  + +I+HPRFSS +V  
Sbjct: 22  VEEEQPLSPAARVFHAPEFNCYVISVIGIKKKIDPDVIIEGLKQT-LIRHPRFSSKMVST 81

Query: 61  RNG-----VEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSK 120
             G      + W RT++ V  HVI VSD  + ++   N +     Y+++L  +  +D SK
Sbjct: 82  SVGNKKRQTQSWVRTNVVVTDHVI-VSDIQTQNIENGNADAFLETYVSNLT-TVPLDISK 141

Query: 121 PLWEIHLL-----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAV 180
           PLW++HLL      A N AV + HH+LGDG+SLM+L L C R+  +PD LP++ +  ++ 
Sbjct: 142 PLWQLHLLDLKTSDAENVAVLKFHHSLGDGMSLMALVLACMRKTSNPDELPSLPNQNRSS 201

Query: 181 RTGNR---GRRSCGEMMLEFLLTVWFSLLFV-------LEFIVRALWVCDRKTPISGGDG 240
              +R   G R      L  ++ +W +++ V       LEFI   +++ D +TPI G   
Sbjct: 202 SRSSRLMAGSRG-DSRFLWLVMVIWSAIMLVLNTVCDALEFIATTMFLKDTETPIKGDFR 261

Query: 241 VELWPRKVATAK-FALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEH----------- 300
                R     +  +L+D+K +K  +   T+NDV+  V  AGLS+YL+            
Sbjct: 262 FSKSKRMCLVHRTVSLDDIKLIKNTM-KMTVNDVVLGVSQAGLSQYLDRRYGEKKKKVGE 321

Query: 301 -----RQPKGLKEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYY 360
                R+   + + ++L    +VNLR   G+QDL+DMM      RWGN +G ++ P +  
Sbjct: 322 DQDSKRKATDMPKRIRLRSALLVNLRPNTGIQDLADMMAKGSTCRWGNWIGYIVFPFSIG 381

Query: 361 TKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTI 420
            +  DPLQ+++R K+++DRKK + EA  ++  GK ++   G +VA  +  R + NT+ + 
Sbjct: 382 LRD-DPLQHLRRAKRIIDRKKNSLEAALTFVAGKFILKTFGVQVAAKIINRALSNTTMSF 441

Query: 421 SNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLA 457
           SN+IGP EEI+  G+P+TY+  +     HALTMH  SY  +  + + V   +I DP  L 
Sbjct: 442 SNLIGPIEEISFYGHPITYMAPSVYGHPHALTMHFQSYMNQMTISLTVDPTVISDPHRLL 501

BLAST of CSPI07G07530 vs. ExPASy Swiss-Prot
Match: Q9C7H4 (Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=WSD2 PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.4e-65
Identity = 168/473 (35.52%), Postives = 249/473 (52.64%), Query Frame = 0

Query: 3   SDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVR--- 62
           ++EP++P  RLF  P ++      +G K   +  ++   I ++ +I HPRFSS+LV    
Sbjct: 10  AEEPVSPFARLFSLPGLDVFNIVTIGCKTEGNASTIVEGIKNT-LINHPRFSSILVTGHG 69

Query: 63  DRNGVEYWRRTSIEVDRHVIVVS-DPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLW 122
           +  G   W  T I V+ HVIV   DP  +     N ++   +Y +++A+ S MD SKPLW
Sbjct: 70  EHKGKARWIPTKINVEEHVIVPDIDPNIE-----NPDEFLEDYTSNMAL-SPMDMSKPLW 129

Query: 123 EIHLLL-----AHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTG 182
           E HLL      A    V R HH+LGDG+SLMSL L C R+  DP+A PT V+  K     
Sbjct: 130 EFHLLKLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPEAFPTFVAPKK----- 189

Query: 183 NRGRRSCGEMM--LEFLLTVWF-SLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVAT 242
           N+ +  C  ++  L F++ + F + + V++ IV      D    I G  G  L   K   
Sbjct: 190 NKAKNVCFSLVAWLWFIVRLMFHTCVEVIKSIVFICRASDTSAHIMGKPGATLSANKFIH 249

Query: 243 AKFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEHR-----QPKGLK--EGLQLTGV 302
              +L+D+K VK  + N T+NDVLF ++ AGLSRYL  R       K  K    + L GV
Sbjct: 250 RIISLDDVKMVKNAM-NMTVNDVLFGMVQAGLSRYLNQRYDLETSSKSRKNLHNIGLHGV 309

Query: 303 AMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRK 362
              NLR    ++DL+ MM      RWGN +G +L+P+    +  D  +YV++ K ++D K
Sbjct: 310 VFFNLRPNRNIEDLAKMMAKGSKCRWGNSIGYVLIPLGMKPQD-DVFEYVRQAKTIMDGK 369

Query: 363 KRTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYI 422
           K + E  FSYG+ K+ M   G +    L  RI  +T+   SNV+GP EEI+  G+ + YI
Sbjct: 370 KHSLEPLFSYGLLKVTMEVFGLRGLKTLVKRIFGSTTMIFSNVVGPDEEISFFGHRIAYI 429

Query: 423 RVTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTA 457
             ++  +  AL + + SY  +  + I V  D+IPDP  L +    AL  M +A
Sbjct: 430 AASTFGVPQALNICIQSYVDKLIINIGVDVDVIPDPHHLCDLIIEALRMMNSA 468

BLAST of CSPI07G07530 vs. ExPASy TrEMBL
Match: A0A0A0K4Q2 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G073550 PE=4 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 1.9e-267
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD
Sbjct: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
           RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI
Sbjct: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC 180
           HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC
Sbjct: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC 180

Query: 181 GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA 240
           GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA
Sbjct: 181 GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA 240

Query: 241 VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM 300
           VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM
Sbjct: 241 VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM 300

Query: 301 MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM 360
           MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM
Sbjct: 301 MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM 360

Query: 361 SFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMS 420
           SFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMS
Sbjct: 361 SFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMS 420

Query: 421 YAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 464
           YAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK
Sbjct: 421 YAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 463

BLAST of CSPI07G07530 vs. ExPASy TrEMBL
Match: A0A5D3C8S5 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00600 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 6.0e-253
Identity = 444/464 (95.69%), Postives = 453/464 (97.63%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           MNSDEPLTPAGRLFLRPEINQIIHCLVG++NSIDVDSVKSQIADSIMIQHPRFSSLLVRD
Sbjct: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGVQNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
           RNGVEYWRRTSIEVDRHVIVVSD VSDDV GVNDEKAANEYLADLAISSSMDYSKPLWEI
Sbjct: 61  RNGVEYWRRTSIEVDRHVIVVSDRVSDDV-GVNDEKAANEYLADLAISSSMDYSKPLWEI 120

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR-TGNRGRRS 180
           HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR T NRGRRS
Sbjct: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRSTRNRGRRS 180

Query: 181 CGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMK 240
            GEMMLEFLL VWFSLLFVLEFI RALWVCDRKTPISGG+GVELWPRKVATAKFALEDMK
Sbjct: 181 LGEMMLEFLLAVWFSLLFVLEFIGRALWVCDRKTPISGGEGVELWPRKVATAKFALEDMK 240

Query: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSD 300
           AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDL+D
Sbjct: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLAD 300

Query: 301 MMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLV 360
           MMKGN+GSRWGNKLGILLLPVNYY K LDPLQYV+RTKKMLDRKKRTFEAHFSYGIGKLV
Sbjct: 301 MMKGNRGSRWGNKLGILLLPVNYYKKTLDPLQYVERTKKMLDRKKRTFEAHFSYGIGKLV 360

Query: 361 MSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMM 420
           MSFLGPKVACILN+RIVCNTSFTISNVIGPREEITIGGN +TYIRVTSTSLSHALTMHMM
Sbjct: 361 MSFLGPKVACILNFRIVCNTSFTISNVIGPREEITIGGNLITYIRVTSTSLSHALTMHMM 420

Query: 421 SYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 464
           SYAGRAEMQ+LVAKDIIPDPEFLAECFENALLEMKTA ATLTTK
Sbjct: 421 SYAGRAEMQVLVAKDIIPDPEFLAECFENALLEMKTAVATLTTK 463

BLAST of CSPI07G07530 vs. ExPASy TrEMBL
Match: A0A1S3BZB1 (Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495247 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 6.0e-253
Identity = 444/464 (95.69%), Postives = 453/464 (97.63%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           MNSDEPLTPAGRLFLRPEINQIIHCLVG++NSIDVDSVKSQIADSIMIQHPRFSSLLVRD
Sbjct: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGVQNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
           RNGVEYWRRTSIEVDRHVIVVSD VSDDV GVNDEKAANEYLADLAISSSMDYSKPLWEI
Sbjct: 61  RNGVEYWRRTSIEVDRHVIVVSDRVSDDV-GVNDEKAANEYLADLAISSSMDYSKPLWEI 120

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR-TGNRGRRS 180
           HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR T NRGRRS
Sbjct: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRSTRNRGRRS 180

Query: 181 CGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMK 240
            GEMMLEFLL VWFSLLFVLEFI RALWVCDRKTPISGG+GVELWPRKVATAKFALEDMK
Sbjct: 181 LGEMMLEFLLAVWFSLLFVLEFIGRALWVCDRKTPISGGEGVELWPRKVATAKFALEDMK 240

Query: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSD 300
           AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDL+D
Sbjct: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLAD 300

Query: 301 MMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLV 360
           MMKGN+GSRWGNKLGILLLPVNYY K LDPLQYV+RTKKMLDRKKRTFEAHFSYGIGKLV
Sbjct: 301 MMKGNRGSRWGNKLGILLLPVNYYKKTLDPLQYVERTKKMLDRKKRTFEAHFSYGIGKLV 360

Query: 361 MSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMM 420
           MSFLGPKVACILN+RIVCNTSFTISNVIGPREEITIGGN +TYIRVTSTSLSHALTMHMM
Sbjct: 361 MSFLGPKVACILNFRIVCNTSFTISNVIGPREEITIGGNLITYIRVTSTSLSHALTMHMM 420

Query: 421 SYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 464
           SYAGRAEMQ+LVAKDIIPDPEFLAECFENALLEMKTA ATLTTK
Sbjct: 421 SYAGRAEMQVLVAKDIIPDPEFLAECFENALLEMKTAVATLTTK 463

BLAST of CSPI07G07530 vs. ExPASy TrEMBL
Match: A0A6J1EMC4 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434667 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.6e-220
Identity = 377/465 (81.08%), Postives = 414/465 (89.03%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           + SD+PLTPAGRLFLRPEINQIIHC++GL+NSIDVDS+KSQIADS+MIQHPRFSSLLVRD
Sbjct: 7   LESDQPLTPAGRLFLRPEINQIIHCVIGLQNSIDVDSIKSQIADSVMIQHPRFSSLLVRD 66

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
            NGVEYWRRTSIEVDRHVIVVSDPVSDD  G  +E A N+YLADL+ SS+MD +KPLWEI
Sbjct: 67  PNGVEYWRRTSIEVDRHVIVVSDPVSDD--GTGNENAVNDYLADLSTSSTMDMNKPLWEI 126

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGR--R 180
           HLLLAHNC +FRIHHALGDGISLMSLFLTCCRRADDP+ALPTIVSD K  R   R R   
Sbjct: 127 HLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPNALPTIVSDSKTARKSRRQRWAE 186

Query: 181 SCGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDM 240
              +M+LE L+T+WFS LFV EFI+RALWV DRKTPISGGDGVELWPRKVATAKFA+EDM
Sbjct: 187 ELPKMLLELLMTLWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDM 246

Query: 241 KAVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLS 300
           KAVK  V NATINDVLFSVIGAGLSRYLEHRQP  LKEGLQLTGVAM+NLREQPGLQDL+
Sbjct: 247 KAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQDLA 306

Query: 301 DMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKL 360
           DMMKGN GSRWGNKLGILLLP+ Y+ K  DPLQY+KRTKKM+DRKKR+ E++FSYGIGK 
Sbjct: 307 DMMKGNNGSRWGNKLGILLLPIYYHKKTSDPLQYLKRTKKMIDRKKRSLESYFSYGIGKF 366

Query: 361 VMSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHM 420
           VMS+LG KVACILNYRI+CNTSFTISNVIGP+EEI++GGNP+TYIR TSTSL HA+TMHM
Sbjct: 367 VMSWLGAKVACILNYRIMCNTSFTISNVIGPKEEISVGGNPITYIRATSTSLPHAITMHM 426

Query: 421 MSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 464
           MSYAGRA+MQILVAKDIIPDPEFL ECFENAL EMK A  T   K
Sbjct: 427 MSYAGRADMQILVAKDIIPDPEFLVECFENALFEMKMAATTFNVK 469

BLAST of CSPI07G07530 vs. ExPASy TrEMBL
Match: A0A6J1JI63 (Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111486010 PE=4 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.5e-219
Identity = 378/465 (81.29%), Postives = 415/465 (89.25%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           + SD+PLTPAGRLFLRPEINQIIHC++GL+NSIDVDS+KSQIADS+MIQHPRFSSLLVRD
Sbjct: 7   LESDQPLTPAGRLFLRPEINQIIHCVIGLRNSIDVDSIKSQIADSVMIQHPRFSSLLVRD 66

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
            NGVEYWRRTSIEVDRHVIVVSDPVSDD  G ++E A N+YLADL+ SS+MD +KPLWEI
Sbjct: 67  PNGVEYWRRTSIEVDRHVIVVSDPVSDD--GASNENAVNDYLADLSTSSTMDINKPLWEI 126

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGR--R 180
           HLLLAHNC +FRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSD K  R   + R   
Sbjct: 127 HLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDSKTARKARQKRWAE 186

Query: 181 SCGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDM 240
              +M+L  L+TVWFS LFV EFI+RALWV DRKTPISGGDGVELWPRKVATAKFA+EDM
Sbjct: 187 ELPKMLLALLMTVWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDM 246

Query: 241 KAVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLS 300
           KAVK  V NATINDVLFSVIGAGLSRYLEHRQP  LKEGLQLTGVAM+NLREQPGLQDL+
Sbjct: 247 KAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQDLA 306

Query: 301 DMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKL 360
           DMMKGN GSRWGNKLGILLLPV Y+ K  DPLQY+KRTKKM+DRKKR+ E++FSYGIGK 
Sbjct: 307 DMMKGNNGSRWGNKLGILLLPVYYHKKMSDPLQYMKRTKKMIDRKKRSLESYFSYGIGKF 366

Query: 361 VMSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHM 420
           VMS+LG KVACILNYRI+CNTSFTISNVIGP+EEI++GGNP+TYIR TSTSL HA+TMHM
Sbjct: 367 VMSWLGAKVACILNYRIMCNTSFTISNVIGPKEEISVGGNPITYIRATSTSLPHAITMHM 426

Query: 421 MSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 464
           MSYAGRA+MQILVAKDII DPEFLA+CFENAL EMK A  TL  K
Sbjct: 427 MSYAGRADMQILVAKDIIHDPEFLAKCFENALFEMKMAATTLNVK 469

BLAST of CSPI07G07530 vs. NCBI nr
Match: XP_004136970.1 (O-acyltransferase WSD1 [Cucumis sativus] >KGN43919.1 hypothetical protein Csa_017278 [Cucumis sativus])

HSP 1 Score: 931.0 bits (2405), Expect = 3.9e-267
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD
Sbjct: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
           RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI
Sbjct: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC 180
           HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC
Sbjct: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC 180

Query: 181 GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA 240
           GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA
Sbjct: 181 GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA 240

Query: 241 VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM 300
           VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM
Sbjct: 241 VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM 300

Query: 301 MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM 360
           MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM
Sbjct: 301 MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM 360

Query: 361 SFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMS 420
           SFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMS
Sbjct: 361 SFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMS 420

Query: 421 YAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 464
           YAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK
Sbjct: 421 YAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 463

BLAST of CSPI07G07530 vs. NCBI nr
Match: XP_008454962.1 (PREDICTED: O-acyltransferase WSD1-like isoform X1 [Cucumis melo] >KAA0031307.1 O-acyltransferase WSD1-like isoform X1 [Cucumis melo var. makuwa] >TYK06759.1 O-acyltransferase WSD1-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 882.9 bits (2280), Expect = 1.2e-252
Identity = 444/464 (95.69%), Postives = 453/464 (97.63%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           MNSDEPLTPAGRLFLRPEINQIIHCLVG++NSIDVDSVKSQIADSIMIQHPRFSSLLVRD
Sbjct: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGVQNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
           RNGVEYWRRTSIEVDRHVIVVSD VSDDV GVNDEKAANEYLADLAISSSMDYSKPLWEI
Sbjct: 61  RNGVEYWRRTSIEVDRHVIVVSDRVSDDV-GVNDEKAANEYLADLAISSSMDYSKPLWEI 120

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR-TGNRGRRS 180
           HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR T NRGRRS
Sbjct: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRSTRNRGRRS 180

Query: 181 CGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMK 240
            GEMMLEFLL VWFSLLFVLEFI RALWVCDRKTPISGG+GVELWPRKVATAKFALEDMK
Sbjct: 181 LGEMMLEFLLAVWFSLLFVLEFIGRALWVCDRKTPISGGEGVELWPRKVATAKFALEDMK 240

Query: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSD 300
           AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDL+D
Sbjct: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLAD 300

Query: 301 MMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLV 360
           MMKGN+GSRWGNKLGILLLPVNYY K LDPLQYV+RTKKMLDRKKRTFEAHFSYGIGKLV
Sbjct: 301 MMKGNRGSRWGNKLGILLLPVNYYKKTLDPLQYVERTKKMLDRKKRTFEAHFSYGIGKLV 360

Query: 361 MSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMM 420
           MSFLGPKVACILN+RIVCNTSFTISNVIGPREEITIGGN +TYIRVTSTSLSHALTMHMM
Sbjct: 361 MSFLGPKVACILNFRIVCNTSFTISNVIGPREEITIGGNLITYIRVTSTSLSHALTMHMM 420

Query: 421 SYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 464
           SYAGRAEMQ+LVAKDIIPDPEFLAECFENALLEMKTA ATLTTK
Sbjct: 421 SYAGRAEMQVLVAKDIIPDPEFLAECFENALLEMKTAVATLTTK 463

BLAST of CSPI07G07530 vs. NCBI nr
Match: XP_038888558.1 (O-acyltransferase WSD1-like [Benincasa hispida])

HSP 1 Score: 805.8 bits (2080), Expect = 1.9e-229
Identity = 401/464 (86.42%), Postives = 427/464 (92.03%), Query Frame = 0

Query: 3   SDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRN 62
           +DEPLTPAGRLFLRPEINQIIHC+VGL+NSID+DSVKSQIADS+MIQHPRFSSLLVRDRN
Sbjct: 32  ADEPLTPAGRLFLRPEINQIIHCVVGLQNSIDIDSVKSQIADSVMIQHPRFSSLLVRDRN 91

Query: 63  GVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHL 122
           GVEYWRRTSIE+DRHVIVV++ VSD+  GV+DEKA N+YLADLAIS SMD  KPLWEIHL
Sbjct: 92  GVEYWRRTSIEIDRHVIVVAERVSDE--GVDDEKAVNQYLADLAISPSMDADKPLWEIHL 151

Query: 123 LLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSCGE 182
           LLAHNCAVFRIHHALGDGISLMS+FLTCCRRADDP+ALPTIVS+ KAVR   R R    E
Sbjct: 152 LLAHNCAVFRIHHALGDGISLMSMFLTCCRRADDPNALPTIVSNSKAVRRNMRQRSWADE 211

Query: 183 ---MMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMK 242
              M+LEFL  VWFS  FV EFI+RALWV DRKTPISGGDGVELWPRKVATAKFA+EDMK
Sbjct: 212 IPKMLLEFLFAVWFSFWFVAEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDMK 271

Query: 243 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSD 302
           AVKK VPNATIND+LFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDL++
Sbjct: 272 AVKKAVPNATINDILFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLTN 331

Query: 303 MMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLV 362
           MMKGN+GSRWGNKLG+LLLPVN + K LDPLQYVK+TKK+LDRKKRT EAHFSYGIGKLV
Sbjct: 332 MMKGNRGSRWGNKLGVLLLPVNCHKKMLDPLQYVKKTKKLLDRKKRTLEAHFSYGIGKLV 391

Query: 363 MSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMM 422
           MS LGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIR TSTSLSHALTMHMM
Sbjct: 392 MSCLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRATSTSLSHALTMHMM 451

Query: 423 SYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 464
           SYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKT+     TK
Sbjct: 452 SYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTSAEVFNTK 493

BLAST of CSPI07G07530 vs. NCBI nr
Match: KAG7022457.1 (O-acyltransferase WSD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 774.2 bits (1998), Expect = 6.2e-220
Identity = 379/465 (81.51%), Postives = 415/465 (89.25%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           + SD+PLTPAGRLFLRPEINQIIHC++GL+NSIDVDS+KSQIADS+MIQHPRFSSLLVRD
Sbjct: 7   LESDQPLTPAGRLFLRPEINQIIHCVIGLQNSIDVDSIKSQIADSVMIQHPRFSSLLVRD 66

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
            NGVEYWRRTSIEVDRHVIVVSDPVS+D  G  +E A N+YLADL+ SS+MD +KPLWEI
Sbjct: 67  PNGVEYWRRTSIEVDRHVIVVSDPVSND--GTGNENAVNDYLADLSTSSTMDMNKPLWEI 126

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGR--R 180
           HLLLAHNC +FRIHHALGDGISLMSLFLTCCRRADDP+ALPTIVSD K  R   R R   
Sbjct: 127 HLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPNALPTIVSDSKTARKSRRQRWAE 186

Query: 181 SCGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDM 240
              +M+LE L+TVWFS LFV EFI+RALWV DRKTPISGGDGVELWPRKVATAKFA+EDM
Sbjct: 187 ELPKMLLELLMTVWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDM 246

Query: 241 KAVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLS 300
           KAVK  V NATINDVLFSVIGAGLSRYLEHRQP  LKEGLQLTGVAM+NLREQPGLQDL+
Sbjct: 247 KAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQDLA 306

Query: 301 DMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKL 360
           DMMKGN GSRWGNKLGILLLPV Y+ K  DPLQY+KRTKKM+DRKKR+ E++FSYGIGK 
Sbjct: 307 DMMKGNNGSRWGNKLGILLLPVYYHKKTSDPLQYLKRTKKMIDRKKRSLESYFSYGIGKF 366

Query: 361 VMSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHM 420
           VMS+LG KVACILNYRI+CNTSFTISNVIGP+EEI++GGNP+TYIR TSTSL HA+TMHM
Sbjct: 367 VMSWLGAKVACILNYRIMCNTSFTISNVIGPKEEISVGGNPITYIRATSTSLPHAITMHM 426

Query: 421 MSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 464
           MSYAGRA+MQILVAKDIIPDPEFLAECFENAL EMK A  T   K
Sbjct: 427 MSYAGRADMQILVAKDIIPDPEFLAECFENALFEMKMAATTFNVK 469

BLAST of CSPI07G07530 vs. NCBI nr
Match: XP_022927903.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 773.1 bits (1995), Expect = 1.4e-219
Identity = 377/465 (81.08%), Postives = 414/465 (89.03%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           + SD+PLTPAGRLFLRPEINQIIHC++GL+NSIDVDS+KSQIADS+MIQHPRFSSLLVRD
Sbjct: 7   LESDQPLTPAGRLFLRPEINQIIHCVIGLQNSIDVDSIKSQIADSVMIQHPRFSSLLVRD 66

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
            NGVEYWRRTSIEVDRHVIVVSDPVSDD  G  +E A N+YLADL+ SS+MD +KPLWEI
Sbjct: 67  PNGVEYWRRTSIEVDRHVIVVSDPVSDD--GTGNENAVNDYLADLSTSSTMDMNKPLWEI 126

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGR--R 180
           HLLLAHNC +FRIHHALGDGISLMSLFLTCCRRADDP+ALPTIVSD K  R   R R   
Sbjct: 127 HLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPNALPTIVSDSKTARKSRRQRWAE 186

Query: 181 SCGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDM 240
              +M+LE L+T+WFS LFV EFI+RALWV DRKTPISGGDGVELWPRKVATAKFA+EDM
Sbjct: 187 ELPKMLLELLMTLWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDM 246

Query: 241 KAVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLS 300
           KAVK  V NATINDVLFSVIGAGLSRYLEHRQP  LKEGLQLTGVAM+NLREQPGLQDL+
Sbjct: 247 KAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQDLA 306

Query: 301 DMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKL 360
           DMMKGN GSRWGNKLGILLLP+ Y+ K  DPLQY+KRTKKM+DRKKR+ E++FSYGIGK 
Sbjct: 307 DMMKGNNGSRWGNKLGILLLPIYYHKKTSDPLQYLKRTKKMIDRKKRSLESYFSYGIGKF 366

Query: 361 VMSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHM 420
           VMS+LG KVACILNYRI+CNTSFTISNVIGP+EEI++GGNP+TYIR TSTSL HA+TMHM
Sbjct: 367 VMSWLGAKVACILNYRIMCNTSFTISNVIGPKEEISVGGNPITYIRATSTSLPHAITMHM 426

Query: 421 MSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 464
           MSYAGRA+MQILVAKDIIPDPEFL ECFENAL EMK A  T   K
Sbjct: 427 MSYAGRADMQILVAKDIIPDPEFLVECFENALFEMKMAATTFNVK 469

BLAST of CSPI07G07530 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 280.4 bits (716), Expect = 2.6e-75
Identity = 175/484 (36.16%), Postives = 271/484 (55.99%), Query Frame = 0

Query: 4   DEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNG 63
           ++PL+PA RLF  PE N  I  +VGLKN I+ D +   I  ++M +HPRFSS LV + N 
Sbjct: 25  EQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVNNCNN 84

Query: 64  ---VEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 123
               + W RT++ V+ HVI+     +  +   N +     Y++DL  +  +D SKPLWE+
Sbjct: 85  NRQEQKWVRTNVVVEDHVIIPKIQ-TQHIENANADVFLESYVSDLT-TIPLDTSKPLWEV 144

Query: 124 HLL-----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTI------VSDLKA 183
           HLL      A N AV RIHH+LGDG+S+MSL L C R+  +P+ LP++       S   +
Sbjct: 145 HLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSGSSS 204

Query: 184 VRTGNRGRRSCGEMMLEFLLTVWFSLLFV-------LEFIVRALWVCDRKTPISGGDGVE 243
           ++T +R    C       ++ +W + L V       LEFI  AL++ D +TPI G   + 
Sbjct: 205 LKTSSR----CYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIKGDFKLS 264

Query: 244 LWPRKVATAK-FALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEH---------RQPK 303
              R     +  +L+D+K +K  +   T+NDV+  V  AGLS+YL+          R   
Sbjct: 265 KGKRMCMVHRTVSLDDIKLIKNAM-KMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNSS 324

Query: 304 GLKEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKAL--DPL 363
            + +G++L    +VNLR   G+QDL+DMM      RWGN +G ++ P   ++ AL  DPL
Sbjct: 325 NIPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFP---FSIALCDDPL 384

Query: 364 QYVKRTKKMLDRKKRTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTISNVIGPR 423
           ++++R K  +DRKK + EA  ++ +GK++++ LG + A  +  R + NT+ + SN++GP 
Sbjct: 385 KHLRRAKSTIDRKKNSLEAVLTFVVGKILLNTLGVQRAANVLNRALSNTTMSFSNLVGPV 444

Query: 424 EEITIGGNPVTYIRVTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLAECFENAL 455
           EEI+  G+ VTYI  +     HALTMH  SY  +  + + V   +I DP  L + +E +L
Sbjct: 445 EEISFYGHTVTYIAPSVYGHPHALTMHFQSYMNKLTISLTVDPTVISDPHKLCDDWEESL 497

BLAST of CSPI07G07530 vs. TAIR 10
Match: AT5G53390.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 275.4 bits (703), Expect = 8.4e-74
Identity = 168/472 (35.59%), Postives = 254/472 (53.81%), Query Frame = 0

Query: 4   DEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNG 63
           +EPL+P  RLF  P+ N  I   +G K   D  ++   + +  ++ HPRFSS+L  +   
Sbjct: 14  EEPLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGL-EHTLVNHPRFSSILEMNNGK 73

Query: 64  VEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHLL 123
              W RT ++V+ HVIV    V  D+   N ++   +Y++ L  +  MD SKPLWE+HLL
Sbjct: 74  KPRWVRTKVKVEEHVIV--PDVDPDI--ENPDQYLEDYISKLT-TIPMDLSKPLWEMHLL 133

Query: 124 -----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRR 183
                 A + A+ +IHH+LGDG+SLMSL L C R+  DP+ALPT+    K  R G     
Sbjct: 134 GVKTSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKK--RFGPSCNS 193

Query: 184 SCGEMMLEFLLTVWF--SLLF-----VLEFIVRALWVCDRKTPISGGDGVELWPRKVATA 243
                +    + +WF   LLF     +L F +    + D +TP+    G EL P++    
Sbjct: 194 GFFNKIWWLFVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLAKPGSELIPKRFVHR 253

Query: 244 KFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEHR-------QPKGLKEGLQLTGVA 303
             + +D+K VK  +   T+NDVL  V  AGLSRYL  +       + K     ++L    
Sbjct: 254 IISFDDVKLVKNAM-KMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAI 313

Query: 304 MVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKK 363
           M+NLR   G++ L+DMM      RWGN  G +LLP +   +  DPL+YV++ K  +DRKK
Sbjct: 314 MINLRPNAGIEALADMMAKKSKCRWGNLFGYILLPFSVGLET-DPLEYVRQAKATIDRKK 373

Query: 364 RTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIR 423
            + EA FS    KL++  LG K + +L  +++ +T+ + SNV+GP+EEIT  G+P+ YI 
Sbjct: 374 HSLEAVFSMAFFKLILKVLGLKASVVLVRKVIHSTTLSFSNVVGPKEEITFHGHPLNYIS 433

Query: 424 VTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTA 457
                  HALT+H  +YA +  + +     +IPDP  + +    +L  +K A
Sbjct: 434 PCVFGHPHALTLHFQTYANKVIISVTADPTVIPDPHKMCDDLVESLKMIKAA 475

BLAST of CSPI07G07530 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 273.5 bits (698), Expect = 3.2e-73
Identity = 170/482 (35.27%), Postives = 267/482 (55.39%), Query Frame = 0

Query: 4   DEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNG 63
           ++PL+PA RLF  PE N  I  ++G K+ +D         +S  I+HPRFSS LV D NG
Sbjct: 17  EQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKES-FIRHPRFSSKLVTDENG 76

Query: 64  V-EYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHL 123
             + W RT++ V+ H IV   P        N      +Y++DL +   +D S+PLWE+HL
Sbjct: 77  QNQRWVRTNVVVEDHFIV---PKIKPQNIENSNAFLEDYVSDL-MKIPLDTSRPLWELHL 136

Query: 124 L-----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKA------VR 183
           L      A N AV +IHH++GDG+S+MSL L C R+  +PD LP++    ++      + 
Sbjct: 137 LDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLT 196

Query: 184 TGNRGRRSCGEMMLEFLLTVWFSLLF-------VLEFIVRALWVCDRKTPISGGD-GVEL 243
           TG+R        +L  +  +W +++         LEFIV  L+V D +TPI G     + 
Sbjct: 197 TGSRS----DSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIKGDFLSTKS 256

Query: 244 WPRKVATAKFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYL---------EHRQPKGL 303
              ++     +L+D+K  K  + N TINDV+  V  AGLSRYL         ++R+ K L
Sbjct: 257 KQLRLVHRTVSLDDIKLTKNAM-NMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKL 316

Query: 304 KEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVK 363
            + ++L    +VNLR   G+QDL+DMM+     RWGN  G ++ P +   +  DPL++++
Sbjct: 317 PKRIRLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFPFSIALRD-DPLEHLE 376

Query: 364 RTKKMLDRKKRTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTISNVIGPREEIT 423
             +K + RKK ++ A  +Y   ++++  LG KVA  +  R+V NT+ T SN++GP EE++
Sbjct: 377 IAQKTISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEEVS 436

Query: 424 IGGNPVTYIRVTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMK 457
             G+P+TY   ++    HALT+H  SY  +  + ++V   +I DP  L + +E +L  +K
Sbjct: 437 FYGHPITYFASSAYGHPHALTIHCQSYMNKMTITLIVDPTVISDPHRLCDDWEESLRSIK 487

BLAST of CSPI07G07530 vs. TAIR 10
Match: AT3G49210.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 270.8 bits (691), Expect = 2.1e-72
Identity = 165/493 (33.47%), Postives = 269/493 (54.56%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           +  ++PL+PA R+F  PE N  +  ++G+K  ID D +   +  + +I+HPRFSS +V  
Sbjct: 22  VEEEQPLSPAARVFHAPEFNCYVISVIGIKKKIDPDVIIEGLKQT-LIRHPRFSSKMVST 81

Query: 61  RNG-----VEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSK 120
             G      + W RT++ V  HVI VSD  + ++   N +     Y+++L  +  +D SK
Sbjct: 82  SVGNKKRQTQSWVRTNVVVTDHVI-VSDIQTQNIENGNADAFLETYVSNLT-TVPLDISK 141

Query: 121 PLWEIHLL-----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAV 180
           PLW++HLL      A N AV + HH+LGDG+SLM+L L C R+  +PD LP++ +  ++ 
Sbjct: 142 PLWQLHLLDLKTSDAENVAVLKFHHSLGDGMSLMALVLACMRKTSNPDELPSLPNQNRSS 201

Query: 181 RTGNR---GRRSCGEMMLEFLLTVWFSLLFV-------LEFIVRALWVCDRKTPISGGDG 240
              +R   G R      L  ++ +W +++ V       LEFI   +++ D +TPI G   
Sbjct: 202 SRSSRLMAGSRG-DSRFLWLVMVIWSAIMLVLNTVCDALEFIATTMFLKDTETPIKGDFR 261

Query: 241 VELWPRKVATAK-FALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEH----------- 300
                R     +  +L+D+K +K  +   T+NDV+  V  AGLS+YL+            
Sbjct: 262 FSKSKRMCLVHRTVSLDDIKLIKNTM-KMTVNDVVLGVSQAGLSQYLDRRYGEKKKKVGE 321

Query: 301 -----RQPKGLKEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYY 360
                R+   + + ++L    +VNLR   G+QDL+DMM      RWGN +G ++ P +  
Sbjct: 322 DQDSKRKATDMPKRIRLRSALLVNLRPNTGIQDLADMMAKGSTCRWGNWIGYIVFPFSIG 381

Query: 361 TKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTI 420
            +  DPLQ+++R K+++DRKK + EA  ++  GK ++   G +VA  +  R + NT+ + 
Sbjct: 382 LRD-DPLQHLRRAKRIIDRKKNSLEAALTFVAGKFILKTFGVQVAAKIINRALSNTTMSF 441

Query: 421 SNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLA 457
           SN+IGP EEI+  G+P+TY+  +     HALTMH  SY  +  + + V   +I DP  L 
Sbjct: 442 SNLIGPIEEISFYGHPITYMAPSVYGHPHALTMHFQSYMNQMTISLTVDPTVISDPHRLL 501

BLAST of CSPI07G07530 vs. TAIR 10
Match: AT1G72110.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 251.9 bits (642), Expect = 9.9e-67
Identity = 168/473 (35.52%), Postives = 249/473 (52.64%), Query Frame = 0

Query: 3   SDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVR--- 62
           ++EP++P  RLF  P ++      +G K   +  ++   I ++ +I HPRFSS+LV    
Sbjct: 10  AEEPVSPFARLFSLPGLDVFNIVTIGCKTEGNASTIVEGIKNT-LINHPRFSSILVTGHG 69

Query: 63  DRNGVEYWRRTSIEVDRHVIVVS-DPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLW 122
           +  G   W  T I V+ HVIV   DP  +     N ++   +Y +++A+ S MD SKPLW
Sbjct: 70  EHKGKARWIPTKINVEEHVIVPDIDPNIE-----NPDEFLEDYTSNMAL-SPMDMSKPLW 129

Query: 123 EIHLLL-----AHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTG 182
           E HLL      A    V R HH+LGDG+SLMSL L C R+  DP+A PT V+  K     
Sbjct: 130 EFHLLKLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPEAFPTFVAPKK----- 189

Query: 183 NRGRRSCGEMM--LEFLLTVWF-SLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVAT 242
           N+ +  C  ++  L F++ + F + + V++ IV      D    I G  G  L   K   
Sbjct: 190 NKAKNVCFSLVAWLWFIVRLMFHTCVEVIKSIVFICRASDTSAHIMGKPGATLSANKFIH 249

Query: 243 AKFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEHR-----QPKGLK--EGLQLTGV 302
              +L+D+K VK  + N T+NDVLF ++ AGLSRYL  R       K  K    + L GV
Sbjct: 250 RIISLDDVKMVKNAM-NMTVNDVLFGMVQAGLSRYLNQRYDLETSSKSRKNLHNIGLHGV 309

Query: 303 AMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRK 362
              NLR    ++DL+ MM      RWGN +G +L+P+    +  D  +YV++ K ++D K
Sbjct: 310 VFFNLRPNRNIEDLAKMMAKGSKCRWGNSIGYVLIPLGMKPQD-DVFEYVRQAKTIMDGK 369

Query: 363 KRTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYI 422
           K + E  FSYG+ K+ M   G +    L  RI  +T+   SNV+GP EEI+  G+ + YI
Sbjct: 370 KHSLEPLFSYGLLKVTMEVFGLRGLKTLVKRIFGSTTMIFSNVVGPDEEISFFGHRIAYI 429

Query: 423 RVTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTA 457
             ++  +  AL + + SY  +  + I V  D+IPDP  L +    AL  M +A
Sbjct: 430 AASTFGVPQALNICIQSYVDKLIINIGVDVDVIPDPHHLCDLIIEALRMMNSA 468

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3B23.7e-7436.16Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3... [more]
Q5KS411.2e-7235.59Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=... [more]
Q9M3B34.5e-7235.27Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B12.9e-7133.47Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3... [more]
Q9C7H41.4e-6535.52Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A0A0K4Q21.9e-267100.00Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G073550 PE=4... [more]
A0A5D3C8S56.0e-25395.69Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BZB16.0e-25395.69Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495247 PE=4 SV... [more]
A0A6J1EMC46.6e-22081.08Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434667 P... [more]
A0A6J1JI632.5e-21981.29Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111486010 PE=... [more]
Match NameE-valueIdentityDescription
XP_004136970.13.9e-267100.00O-acyltransferase WSD1 [Cucumis sativus] >KGN43919.1 hypothetical protein Csa_01... [more]
XP_008454962.11.2e-25295.69PREDICTED: O-acyltransferase WSD1-like isoform X1 [Cucumis melo] >KAA0031307.1 O... [more]
XP_038888558.11.9e-22986.42O-acyltransferase WSD1-like [Benincasa hispida][more]
KAG7022457.16.2e-22081.51O-acyltransferase WSD1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022927903.11.4e-21981.08O-acyltransferase WSD1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G49200.12.6e-7536.16O-acyltransferase (WSD1-like) family protein [more]
AT5G53390.18.4e-7435.59O-acyltransferase (WSD1-like) family protein [more]
AT3G49190.13.2e-7335.27O-acyltransferase (WSD1-like) family protein [more]
AT3G49210.12.1e-7233.47O-acyltransferase (WSD1-like) family protein [more]
AT1G72110.19.9e-6735.52O-acyltransferase (WSD1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009721O-acyltransferase WSD1, C-terminalPFAMPF06974WS_DGAT_Ccoord: 309..454
e-value: 1.7E-43
score: 148.1
IPR004255O-acyltransferase, WSD1, N-terminalPFAMPF03007WES_acyltransfcoord: 108..253
e-value: 1.6E-9
score: 37.9
NoneNo IPR availablePANTHERPTHR31650:SF41O-ACYLTRANSFERASE WSD1-LIKE ISOFORM X1coord: 2..458
NoneNo IPR availableSUPERFAMILY52777CoA-dependent acyltransferasescoord: 6..150
IPR045034O-acyltransferase WSD1-likePANTHERPTHR31650O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 2..458

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G07530.1CSPI07G07530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0045017 glycerolipid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity
molecular_function GO:0047196 long-chain-alcohol O-fatty-acyltransferase activity
molecular_function GO:0008374 O-acyltransferase activity