CSPI07G07390 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G07390
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Cysteine protease
Location	Chr7: 5324526 .. 5327587 (-)
RNA-Seq Expression	CSPI07G07390
Synteny	CSPI07G07390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTAATTTAAGCTAAATAATAATATATAATATTTGATTCATATAAATCTATTGGCTAAAATATTTATTTTAATTTAATTATTTAGGCAAAAAATTTAAATTTAGAATCTTTATGGAGAGCCTCTCAGCAACGGCGAAAAAATGGAGTTTTTCAAACAGATTCGAGCTTGCAAATATTCTCTGTAATTTGTGGACTACTTTTTCCCAAATCCCATTTCTTCTTCTCAGCTTTCCTATTTAATTCTCCAATCCTCTTCACTTTTTCCTTCATTCCCATTTCTTCTCTTCTCTTCTCTCCCAAATCCCACCAAACATGGCGATTTCGCCCATTTTCTTCGCTTTTCTTGCCCTTTTCTTCTGTCTTTCCCCTTTTTCATCCGCTTCCCATTCATCCACCTTCTCCATCATCGATGAAAACGCTAAACACCACTTGGGTATTCCTGAGATTCCCCATTCTGATGCCCATCAACGCCCCGACGAGGAAGTTGCCGCTTTGTACGAGTCCTGGTTGGTCCATCATGGGAAAGCCTACAATGCTATTGGTGAGAAGGAGAGACGGTTTGAGATTTTTAAGGATAATCTCAGGTTTATTGATGAACATAACCGGGAATCGCGGACGTATAAAGTTGGATTAACCCGTTTTGCCGATCTTACCAATGAGGAATATCGGGCAAGGTTTTTGGGTGGCCGATTCTCCCGGAAGCCTAGGTTATCTGCCGCCAAGAGTGGTAGGTATGCGGCGGCGCTCGGTGATGATCTTCCGGATGATGTCGATTGGAGAAAAAAGGGCGCTGTTGCTACTGTTAAAGATCAAGGACAGTGTGGTGGGTTTTCTTTTTTATTTTTCTCTCTCTTTTTTTAACTTTTTTTTTTTTTTGTTGAATTGATCCCATGAGAATGATGGTTGGATTTTGTGTTTTCTTTTCGTTTTCTCATTTGGTCTTTGATGTTTTGACTCTTGTTTGGGTCGTACTGATTTTACCAGGAAATTTGTTATGAGATCCATATTTATAGGTAACATTTCCCACAAATAGATTCATTAGATATTGGTCAATACTCAACCTTGATCGTAACTTTTATCTGCTATAATTAGCTTCGCTTCATCTCTGATGATTTGTTGATGACCATCGATTACATCAGAAAACGTATTAGAAACTTTCCGCTCAAAACTTGTTTTCCAAGTAACCTTTTTATCTCGAAGGATTATTTTAGTAAAGGCTTTTGATGGATGGCGATTGACGTAATTGGGTTGTTTTTTGAATGAAGGGAGCTGTTGGGCTTTCTCATCAGTTGCTGCGGTGGAAGGAATAAACCAAATTGTCACTGGCGAATTGATCCCTCTGTCAGAGCAGGAACTTGTTGATTGTGACAAATCATTTAACATGGGTTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGGCAATGGTGGAATTGACACCGAGGAAGATTACCCTTACAAGGGACGTGATGCCGCTTGCGATCCCAATCGGGTTTGTCCCCAATCAGCTTCATTTGATTCATTTATCTTACTATTTATAGTAATTTGACTTGGTTTTTAATGTTTTGTCCAAAGAAAAATGCCAAGGTTGTAACCATAGATGGATATGAAGATGTTCCTGAGAATGATGAGAGCTCCTTGAAAAAGGCGGTGGCAAATCAACCCGTTAGTGTTGCAATTGAAGCTGGTGGCAGAGCCTTCCAACTTTACCAATCGGTAATCCCTTCAACTTTGAGCAATTTCCTGAATGTTGTTTCACAAAGCTTTGGTTTTGACTTCTCTGGCGTTGTAAATGGTATCTGTTTTGGAAGCTTATTTGAACATATTTTGTTGTGAGTTAATTCATCATTCTGGCTTGGGAATCAATATTCGAGTTTAATCTGTTGTGATTGCATGCCTTAAAGACGAAGACAATCTGTATTGATCCACCATTTAGGCTTGGGAAATGAAAGCTATTTCAAACACTTTGTGTTTATGCATCATATAAGGTGTTGTGAGTGCGTGTTTTAAATACAATATGTGTTTATGCATCAATTCAGGGTGTCTTCACTGGCCGTTGTGGAACCGATCTAGATCATGGTGTTGTTGCTGTTGGATATGGAACCGATAACGGTACAGATTATTGGATTGTGAGGAATTCATGGGGTAAAGACTGGGGAGAGAGTGGTTATATCAGGTTGGAGAGAAATGTGGCCAACATTACCACAGGCAAGTGTGGTATTGCAGTACAGCCTTCATACCCCACCAAATCCGGAGCAAACCCTCCAAAACCTTCTGCTTCTCCCCCATCTCCTGTCAAGCCTCCAACTGAATGCGACGAATACTTCTCGTGCGAAGAAGGAAGTACCTGCTGCTGCATCTATCAGTTTGGAAGCACCTGCTTTGCTTGGGGATGCTGCCCCCTCGAGTCCGCAACCTGCTGTGATGACCATTACAGTTGCTGCCCACATGAATATCCCGTCTGTGACCTTGAGGCAGGAACTTGCCGAGTGGTAAGTCACATCACATAGTTTTCTAACCAATGCATATTCTTCAATGAGTTCTTTGTCCTATTAACTCATGATACTCATTCAAGTTCATGTTTGATATAATCTAATCATTGTAAATGGATGAAACACAGAGCAAAGACAGCTCGATGGGCGTCAATCTGCTGAAGCGCCTTCCTGCAATACAGACAAGGAAGGTTCAAAAGCTTGGGAAGTTGTTCGTTGGTGCTTGAGAGGTTTGGCAATGGTGGTCGGTGTCGTGAGTGAAATAATCGACCATTTGAACACAGGTCCATAGTATAGAAAAGTTATTAAGAAGTGGAAGATTGATGTCCCCATTTATTGATCTTTGCTATTGTGGGGCTTATTACTTTCCTTAAAAGTATTGAACCTTGATTATGATATATTCCCATTGCTCTTTCAAATATTGATGGTGGCTTTGTATATAAAGTTTCCTCAGGTGAAAATTTGTAACAAACTTCCATCTCATGTGCTTTGAAGATAAATTGTGTATATATTTATCAATTCTTAGTGGGTCTTGGATGTGGTGGATCTCTTTTGAAATTT

mRNA sequence

TGTAATTTAAGCTAAATAATAATATATAATATTTGATTCATATAAATCTATTGGCTAAAATATTTATTTTAATTTAATTATTTAGGCAAAAAATTTAAATTTAGAATCTTTATGGAGAGCCTCTCAGCAACGGCGAAAAAATGGAGTTTTTCAAACAGATTCGAGCTTGCAAATATTCTCTGTAATTTGTGGACTACTTTTTCCCAAATCCCATTTCTTCTTCTCAGCTTTCCTATTTAATTCTCCAATCCTCTTCACTTTTTCCTTCATTCCCATTTCTTCTCTTCTCTTCTCTCCCAAATCCCACCAAACATGGCGATTTCGCCCATTTTCTTCGCTTTTCTTGCCCTTTTCTTCTGTCTTTCCCCTTTTTCATCCGCTTCCCATTCATCCACCTTCTCCATCATCGATGAAAACGCTAAACACCACTTGGGTATTCCTGAGATTCCCCATTCTGATGCCCATCAACGCCCCGACGAGGAAGTTGCCGCTTTGTACGAGTCCTGGTTGGTCCATCATGGGAAAGCCTACAATGCTATTGGTGAGAAGGAGAGACGGTTTGAGATTTTTAAGGATAATCTCAGGTTTATTGATGAACATAACCGGGAATCGCGGACGTATAAAGTTGGATTAACCCGTTTTGCCGATCTTACCAATGAGGAATATCGGGCAAGGTTTTTGGGTGGCCGATTCTCCCGGAAGCCTAGGTTATCTGCCGCCAAGAGTGGTAGGTATGCGGCGGCGCTCGGTGATGATCTTCCGGATGATGTCGATTGGAGAAAAAAGGGCGCTGTTGCTACTGTTAAAGATCAAGGACAGTGTGGGAGCTGTTGGGCTTTCTCATCAGTTGCTGCGGTGGAAGGAATAAACCAAATTGTCACTGGCGAATTGATCCCTCTGTCAGAGCAGGAACTTGTTGATTGTGACAAATCATTTAACATGGGTTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGGCAATGGTGGAATTGACACCGAGGAAGATTACCCTTACAAGGGACGTGATGCCGCTTGCGATCCCAATCGGAAAAATGCCAAGGTTGTAACCATAGATGGATATGAAGATGTTCCTGAGAATGATGAGAGCTCCTTGAAAAAGGCGGTGGCAAATCAACCCGTTAGTGTTGCAATTGAAGCTGGTGGCAGAGCCTTCCAACTTTACCAATCGGGTGTCTTCACTGGCCGTTGTGGAACCGATCTAGATCATGGTGTTGTTGCTGTTGGATATGGAACCGATAACGGTACAGATTATTGGATTGTGAGGAATTCATGGGGTAAAGACTGGGGAGAGAGTGGTTATATCAGGTTGGAGAGAAATGTGGCCAACATTACCACAGGCAAGTGTGGTATTGCAGTACAGCCTTCATACCCCACCAAATCCGGAGCAAACCCTCCAAAACCTTCTGCTTCTCCCCCATCTCCTGTCAAGCCTCCAACTGAATGCGACGAATACTTCTCGTGCGAAGAAGGAAGTACCTGCTGCTGCATCTATCAGTTTGGAAGCACCTGCTTTGCTTGGGGATGCTGCCCCCTCGAGTCCGCAACCTGCTGTGATGACCATTACAGTTGCTGCCCACATGAATATCCCGTCTGTGACCTTGAGGCAGGAACTTGCCGAGTGAGCAAAGACAGCTCGATGGGCGTCAATCTGCTGAAGCGCCTTCCTGCAATACAGACAAGGAAGGTTCAAAAGCTTGGGAAGTTGTTCGTTGGTGCTTGAGAGGTTTGGCAATGGTGGTCGGTGTCGTGAGTGAAATAATCGACCATTTGAACACAGGTCCATAGTATAGAAAAGTTATTAAGAAGTGGAAGATTGATGTCCCCATTTATTGATCTTTGCTATTGTGGGGCTTATTACTTTCCTTAAAAGTATTGAACCTTGATTATGATATATTCCCATTGCTCTTTCAAATATTGATGGTGGCTTTGTATATAAAGTTTCCTCAGGTGAAAATTTGTAACAAACTTCCATCTCATGTGCTTTGAAGATAAATTGTGTATATATTTATCAATTCTTAGTGGGTCTTGGATGTGGTGGATCTCTTTTGAAATTT

Coding sequence (CDS)

ATGGCGATTTCGCCCATTTTCTTCGCTTTTCTTGCCCTTTTCTTCTGTCTTTCCCCTTTTTCATCCGCTTCCCATTCATCCACCTTCTCCATCATCGATGAAAACGCTAAACACCACTTGGGTATTCCTGAGATTCCCCATTCTGATGCCCATCAACGCCCCGACGAGGAAGTTGCCGCTTTGTACGAGTCCTGGTTGGTCCATCATGGGAAAGCCTACAATGCTATTGGTGAGAAGGAGAGACGGTTTGAGATTTTTAAGGATAATCTCAGGTTTATTGATGAACATAACCGGGAATCGCGGACGTATAAAGTTGGATTAACCCGTTTTGCCGATCTTACCAATGAGGAATATCGGGCAAGGTTTTTGGGTGGCCGATTCTCCCGGAAGCCTAGGTTATCTGCCGCCAAGAGTGGTAGGTATGCGGCGGCGCTCGGTGATGATCTTCCGGATGATGTCGATTGGAGAAAAAAGGGCGCTGTTGCTACTGTTAAAGATCAAGGACAGTGTGGGAGCTGTTGGGCTTTCTCATCAGTTGCTGCGGTGGAAGGAATAAACCAAATTGTCACTGGCGAATTGATCCCTCTGTCAGAGCAGGAACTTGTTGATTGTGACAAATCATTTAACATGGGTTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGGCAATGGTGGAATTGACACCGAGGAAGATTACCCTTACAAGGGACGTGATGCCGCTTGCGATCCCAATCGGAAAAATGCCAAGGTTGTAACCATAGATGGATATGAAGATGTTCCTGAGAATGATGAGAGCTCCTTGAAAAAGGCGGTGGCAAATCAACCCGTTAGTGTTGCAATTGAAGCTGGTGGCAGAGCCTTCCAACTTTACCAATCGGGTGTCTTCACTGGCCGTTGTGGAACCGATCTAGATCATGGTGTTGTTGCTGTTGGATATGGAACCGATAACGGTACAGATTATTGGATTGTGAGGAATTCATGGGGTAAAGACTGGGGAGAGAGTGGTTATATCAGGTTGGAGAGAAATGTGGCCAACATTACCACAGGCAAGTGTGGTATTGCAGTACAGCCTTCATACCCCACCAAATCCGGAGCAAACCCTCCAAAACCTTCTGCTTCTCCCCCATCTCCTGTCAAGCCTCCAACTGAATGCGACGAATACTTCTCGTGCGAAGAAGGAAGTACCTGCTGCTGCATCTATCAGTTTGGAAGCACCTGCTTTGCTTGGGGATGCTGCCCCCTCGAGTCCGCAACCTGCTGTGATGACCATTACAGTTGCTGCCCACATGAATATCCCGTCTGTGACCTTGAGGCAGGAACTTGCCGAGTGAGCAAAGACAGCTCGATGGGCGTCAATCTGCTGAAGCGCCTTCCTGCAATACAGACAAGGAAGGTTCAAAAGCTTGGGAAGTTGTTCGTTGGTGCTTGA

Protein sequence

MAISPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVAAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKGRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRKVQKLGKLFVGA*

Homology

[Close]

BLAST of CSPI07G07390 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 3.3e-179
Identity = 299/453 (66.00%), Postives = 360/453 (79.47%), Query Frame = 0


Query: 13  LFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAALYESWLVHHGKA 72
           LF  +   SSA      SII  + KH +       S    R + EV ++YE+WLV HGKA
Sbjct: 11  LFLAMVAVSSA---VDMSIISYDEKHGV-------STTGGRSEAEVMSIYEAWLVKHGKA 70

Query: 73  --YNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRARFLGGRFSRK 132
              N++ EK+RRFEIFKDNLRF+DEHN ++ +Y++GLTRFADLTN+EYR+++LG +  +K
Sbjct: 71  QSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKK 130

Query: 133 PRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVAAVEGINQIVT 192
                  S RY A +GD+LP+ +DWRKKGAVA VKDQG CGSCWAFS++ AVEGINQIVT
Sbjct: 131 G--ERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVT 190

Query: 193 GELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKGRDAACDPNRK 252
           G+LI LSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDT++DYPYKG D  CD  RK
Sbjct: 191 GDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRK 250

Query: 253 NAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTDLDHG 312
           NAKVVTID YEDVP   E SLKKAVA+QP+S+AIEAGGRAFQLY SG+F G CGT LDHG
Sbjct: 251 NAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHG 310

Query: 313 VVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQPSYPTKSGAN 372
           VVAVGYGT+NG DYWIVRNSWGK WGESGY+R+ RN+A+ ++GKCGIA++PSYP K+G N
Sbjct: 311 VVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIAS-SSGKCGIAIEPSYPIKNGEN 370

Query: 373 PPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLESATCCDDHYSC 432
           PP P  SPPSP+KPPT+CD Y++C E +TCCC++++G  CFAWGCCPLE+ATCCDD+YSC
Sbjct: 371 PPNPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSC 430

Query: 433 CPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPA 464
           CPHEYPVCDL+ GTC +SK+S   V  LKR PA
Sbjct: 431 CPHEYPVCDLDQGTCLLSKNSPFSVKALKRKPA 450

[Close]

BLAST of CSPI07G07390 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 5.3e-177
Identity = 304/462 (65.80%), Postives = 352/462 (76.19%), Query Frame = 0


Query: 8   FAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAALYESWLV 67
           F  L+    L      S++   SII  +  HH+       +    R D EV  +YE+W+V
Sbjct: 3   FLKLSPMILLLAMIGVSYAMDMSIISYDENHHI-------TTETSRSDSEVERIYEAWMV 62

Query: 68  HHGKA---YNAIG-EKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRARFL 127
            HGK     N +G EK++RFEIFKDNLRFIDEHN ++ +YK+GLTRFADLTNEEYR+ +L
Sbjct: 63  EHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYL 122

Query: 128 GGRFSRKPRLSAAK-SGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVAAV 187
           G     KP     K S RY A +GD LPD VDWRK+GAVA VKDQG CGSCWAFS++ AV
Sbjct: 123 GA----KPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAV 182

Query: 188 EGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKGRD 247
           EGIN+IVTG+LI LSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDTE DYPYK  D
Sbjct: 183 EGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAAD 242

Query: 248 AACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGR 307
             CD NRKNAKVVTID YEDVPEN E+SLKKA+A+QP+SVAIEAGGRAFQLY SGVF G 
Sbjct: 243 GRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGL 302

Query: 308 CGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQPS 367
           CGT+LDHGVVAVGYGT+NG DYWIVRNSWG  WGESGYI++ RN+    TGKCGIA++ S
Sbjct: 303 CGTELDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNI-EAPTGKCGIAMEAS 362

Query: 368 YPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLESAT 427
           YP K G NPP P  SPPSP+KPPT CD+YFSC E +TCCC+Y++G  CF WGCCPLE+AT
Sbjct: 363 YPIKKGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAAT 422

Query: 428 CCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAI 465
           CCDD+ SCCPHEYPVCD+  GTC +SK+S   V  LKR PAI
Sbjct: 423 CCDDNSSCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPAI 452

[Close]

BLAST of CSPI07G07390 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 616.3 bits (1588), Expect = 2.9e-175
Identity = 284/413 (68.77%), Postives = 333/413 (80.63%), Query Frame = 0


Query: 52  QRPDEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRES----RTYKVGL 111
           +R +EE   LY  W   HGK+YNA+GE+ERR+  F+DNLR+IDEHN  +     ++++GL
Sbjct: 30  ERSEEEARRLYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEHNAAADAGVHSFRLGL 89

Query: 112 TRFADLTNEEYRARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQ 171
            RFADLTNEEYR  +LG R   KPR     S RY AA  + LP+ VDWR KGAVA +KDQ
Sbjct: 90  NRFADLTNEEYRDTYLGLR--NKPRRERKVSDRYLAADNEALPESVDWRTKGAVAEIKDQ 149

Query: 172 GQCGSCWAFSSVAAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGN 231
           G CGSCWAFS++AAVEGINQIVTG+LI LSEQELVDCD S+N GCNGGLMDYAF FII N
Sbjct: 150 GGCGSCWAFSAIAAVEGINQIVTGDLISLSEQELVDCDTSYNEGCNGGLMDYAFDFIINN 209

Query: 232 GGIDTEEDYPYKGRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAG 291
           GGIDTE+DYPYKG+D  CD NRKNAKVVTID YEDV  N E+SL+KAVANQPVSVAIEAG
Sbjct: 210 GGIDTEDDYPYKGKDERCDVNRKNAKVVTIDSYEDVTPNSETSLQKAVANQPVSVAIEAG 269

Query: 292 GRAFQLYQSGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNV 351
           GRAFQLY SG+FTG+CGT LDHGV AVGYGT+NG DYWIVRNSWGK WGESGY+R+ERN+
Sbjct: 270 GRAFQLYSSGIFTGKCGTALDHGVAAVGYGTENGKDYWIVRNSWGKSWGESGYVRMERNI 329

Query: 352 ANITTGKCGIAVQPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFG 411
              ++GKCGIAV+PSYP K G NPP P  +PPSP  PPT CD Y++C + +TCCCIY++G
Sbjct: 330 -KASSGKCGIAVEPSYPLKKGENPPNPGPTPPSPTPPPTVCDNYYTCPDSTTCCCIYEYG 389

Query: 412 STCFAWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKR 461
             C+AWGCCPLE ATCCDDHYSCCPHEYP+C+++ GTC ++KDS + V  LKR
Sbjct: 390 KYCYAWGCCPLEGATCCDDHYSCCPHEYPICNVQQGTCLMAKDSPLAVKALKR 439

[Close]

BLAST of CSPI07G07390 vs. ExPASy Swiss-Prot
Match: Q9LT78 (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 1.6e-157
Identity = 272/419 (64.92%), Postives = 322/419 (76.85%), Query Frame = 0


Query: 53  RPDEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNR-ESRTYKVGLTRFA 112
           R + E   +YE WLV + K YN +GEKERRFEIFKDNL+F++EH+   +RTY+VGLTRFA
Sbjct: 34  RNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFA 93

Query: 113 DLTNEEYRARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCG 172
           DLTN+E+RA +L  +  R       K  +Y   +GD LPD +DWR KGAV  VKDQG CG
Sbjct: 94  DLTNDEFRAIYLRSKMER--TRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCG 153

Query: 173 SCWAFSSVAAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGID 232
           SCWAFS++ AVEGINQI TGELI LSEQELVDCD S+N GC GGLMDYAF+FII NGGID
Sbjct: 154 SCWAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGID 213

Query: 233 TEEDYPYKGRDA-ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRA 292
           TEEDYPY   D   C+ ++KN +VVTIDGYEDVP+NDE SLKKA+ANQP+SVAIEAGGRA
Sbjct: 214 TEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRA 273

Query: 293 FQLYQSGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANI 352
           FQLY SGVFTG CGT LDHGVVAVGYG++ G DYWIVRNSWG +WGESGY +LERN+   
Sbjct: 274 FQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKE- 333

Query: 353 TTGKCGIAVQPSYPTK-SGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGST 412
           ++GKCG+A+  SYPTK SG+NPPKP A  PSPV     CD+  +C   STCCC+Y++   
Sbjct: 334 SSGKCGVAMMASYPTKSSGSNPPKPPA--PSPV----VCDKSNTCPAKSTCCCLYEYNGK 393

Query: 413 CFAWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRK 469
           C++WGCCP ESATCCDD  SCCP  YPVCDL+A TCR+  +S + +  L R PAI T K
Sbjct: 394 CYSWGCCPYESATCCDDGSSCCPQSYPVCDLKANTCRMKGNSPLSIKALTRGPAIATTK 443

[Close]

BLAST of CSPI07G07390 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 549.3 bits (1414), Expect = 4.4e-155
Identity = 283/465 (60.86%), Postives = 337/465 (72.47%), Query Frame = 0


Query: 9   AFLALFFCLSPFSSASHSSTFSIIDENAKHHL-GIPEIPHSDAHQRPDEEVAALYESWLV 68
           A  A F  L    +A+ +   SII  NA+H   G+ E P        + E  A Y+ WL 
Sbjct: 5   AAAAAFLLLLIVGAATAAPDMSIISYNAEHGARGLEEGP-------TEAEARAAYDLWLA 64

Query: 69  HH-GKAYNAI-GEKERRFEIFKDNLRFIDEHNR---ESRTYKVGLTRFADLTNEEYRARF 128
            + G + NA+ GE ERRF +F DNL+F+D HN    E   +++G+ RFADLTNEE+RA F
Sbjct: 65  ENGGGSPNALGGEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNEEFRATF 124

Query: 129 LGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVAAV 188
           LG + + + R   A   RY     ++LP+ VDWR+KGAVA VK+QGQCGSCWAFS+V+ V
Sbjct: 125 LGAKVAERSR---AAGERYRHDGVEELPESVDWREKGAVAPVKNQGQCGSCWAFSAVSTV 184

Query: 189 EGINQIVTGELIPLSEQELVDCD-KSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKGR 248
           E INQ+VTGE+I LSEQELV+C     N GCNGGLMD AF FII NGGIDTE+DYPYK  
Sbjct: 185 ESINQLVTGEMITLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNGGIDTEDDYPYKAV 244

Query: 249 DAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTG 308
           D  CD NR+NAKVV+IDG+EDVP+NDE SL+KAVA+QPVSVAIEAGGR FQLY SGVF+G
Sbjct: 245 DGKCDINRENAKVVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYHSGVFSG 304

Query: 309 RCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQP 368
           RCGT LDHGVVAVGYGTDNG DYWIVRNSWG  WGESGY+R+ERN+ N+TTGKCGIA+  
Sbjct: 305 RCGTSLDHGVVAVGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNI-NVTTGKCGIAMMA 364

Query: 369 SYPTKSGANPPKPSASPPSPVKPPTE------CDEYFSCEEGSTCCCIYQFGSTCFAWGC 428
           SYPTKSGANPPKPS +PP+P  PP        CD+ FSC  GSTCCC + F + C  WGC
Sbjct: 365 SYPTKSGANPPKPSPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCCCAFGFRNLCLVWGC 424

Query: 429 CPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKR 461
           CP+E ATCC DH SCCP +YPVC+  AGTC  SK+S + V  LKR
Sbjct: 425 CPVEGATCCKDHASCCPPDYPVCNTRAGTCSASKNSPLSVKALKR 458

[Close]

BLAST of CSPI07G07390 vs. ExPASy TrEMBL
Match: A0A0A0K4N3 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_7G073400 PE=3 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 6.1e-285
Identity = 478/479 (99.79%), Postives = 479/479 (100.00%), Query Frame = 0


Query: 1   MAISPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAA 60
           MAISPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAA
Sbjct: 1   MAISPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAA 60

Query: 61  LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRA 120
           LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRA
Sbjct: 61  LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRA 120

Query: 121 RFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVA 180
           RFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVA
Sbjct: 121 RFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVA 180

Query: 181 AVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKG 240
           AVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKG
Sbjct: 181 AVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKG 240

Query: 241 RDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT 300
           RDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT
Sbjct: 241 RDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT 300

Query: 301 GRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQ 360
           GRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQ
Sbjct: 301 GRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQ 360

Query: 361 PSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLES 420
           PSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLES
Sbjct: 361 PSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLES 420

Query: 421 ATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRKVQKLGKLFVGA 480
           ATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQT+KVQKLGKLFVGA
Sbjct: 421 ATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTKKVQKLGKLFVGA 479

[Close]

BLAST of CSPI07G07390 vs. ExPASy TrEMBL
Match: A0A1S3BZW8 (low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC103495259 PE=3 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 4.2e-270
Identity = 453/480 (94.38%), Postives = 465/480 (96.88%), Query Frame = 0


Query: 1   MAI-SPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVA 60
           MAI SPIFFAFLALF CLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQR DEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 ARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSV 180
           ARFLGGRFSRKP LSAAKSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQCGSCWAFS+V
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180

Query: 181 AAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYK 240
           AAVEGINQIVTGELI LSEQELVDCDKSFNMGCNGGLMDYAFQFII NGGIDT+EDYPYK
Sbjct: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240

Query: 241 GRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRD ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAV 360
           TGRCGT+LDHGVVAVGYGTDNGTDYWIVRNSWGKDWGE+GYIRLERNVAN TTGKCGIAV
Sbjct: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360

Query: 361 QPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLE 420
           +PSYP KSG+NPPKPSASPPSPV PPTECDEYFSC+EGSTCCCIYQ+GSTCFAWGCCPLE
Sbjct: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRKVQKLGKLFVGA 480
           SATCCDDHYSCCPHEYPVCDLEAGTCR SKDS MGVNLLKRLPA QT+++QKLGKLFVGA
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA 480

[Close]

BLAST of CSPI07G07390 vs. ExPASy TrEMBL
Match: A0A5A7SJU5 (Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00750 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 9.8e-267
Identity = 452/488 (92.62%), Postives = 464/488 (95.08%), Query Frame = 0


Query: 1   MAI-SPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVA 60
           MAI SPIFFAFLALF CLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQR DEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 ARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQ--------CG 180
           ARFLGGRFSRKP LSAAKSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQ         G
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQKFVMRSIFIG 180

Query: 181 SCWAFSSVAAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGID 240
           SCWAFS+VAAVEGINQIVTGELI LSEQELVDCDKSFNMGCNGGLMDYAFQFII NGGID
Sbjct: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240

Query: 241 TEEDYPYKGRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300
           T+EDYPYKGRD ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF
Sbjct: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300

Query: 301 QLYQSGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANIT 360
           QLYQSGVFTGRCGT+LDHGVVAVGYGTDNGTDYWIVRNSWGKDWGE+GYIRLERNVAN T
Sbjct: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360

Query: 361 TGKCGIAVQPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCF 420
           TGKCGIAV+PSYP KSG+NPPKPSASPPSPV PPTECDEYFSC+EGSTCCCIYQ+GSTCF
Sbjct: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420

Query: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRKVQK 480
           AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCR SKDS MGVNLLKRLPA QT+++QK
Sbjct: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQK 480

[Close]

BLAST of CSPI07G07390 vs. ExPASy TrEMBL
Match: A0A6J1D149 (low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 GN=LOC111016644 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.2e-232
Identity = 388/474 (81.86%), Postives = 425/474 (89.66%), Query Frame = 0


Query: 9   AFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRP---DEEVAALYESW 68
           A LALF C   FSSAS SS+FSIIDENAKHHLG P+I  SDA   P    E+VAALY+SW
Sbjct: 13  ASLALFLCFLSFSSASDSSSFSIIDENAKHHLGFPDIAGSDAGTPPLRTQEQVAALYKSW 72

Query: 69  LVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRARFLGG 128
           LV HGKAYNA+GE+ERRFEIFKDNLRFIDEHNRE R+Y +GLTRFADLTNEEYRARFLGG
Sbjct: 73  LVKHGKAYNALGERERRFEIFKDNLRFIDEHNREPRSYTLGLTRFADLTNEEYRARFLGG 132

Query: 129 RFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVAAVEGI 188
           RFS KPR SAAK+GRYA++LG DLP+ VDWR+KGAV  VKDQGQCGSCWAFS++AAVEGI
Sbjct: 133 RFSPKPRPSAAKNGRYASSLGGDLPEHVDWREKGAVTAVKDQGQCGSCWAFSTIAAVEGI 192

Query: 189 NQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKGRDAAC 248
           NQIVTGELI LSEQELVDCDKS+NMGCNGGLMDYAFQFI+ NGGIDTEEDYPYKGRD  C
Sbjct: 193 NQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIVDNGGIDTEEDYPYKGRDGVC 252

Query: 249 DPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGT 308
           DPNR+NA VVTIDGYEDVPENDE +LKKAVA+QPVSVAIEAGGRAFQLYQSGVFTGRCGT
Sbjct: 253 DPNRRNANVVTIDGYEDVPENDEGALKKAVASQPVSVAIEAGGRAFQLYQSGVFTGRCGT 312

Query: 309 DLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQPSYPT 368
           DLDHGVVAVGYGT+NG DYWIVRNSWGK+WGE+GYIRLERNVANITTGKCGIA++PSYP 
Sbjct: 313 DLDHGVVAVGYGTENGLDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPV 372

Query: 369 KSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLESATCCD 428
           K+G NPPKP+ SPPSPVKPPTECD+Y+SC EG+TCCCIYQ+GSTCF WGCCPLESATCCD
Sbjct: 373 KTGKNPPKPAPSPPSPVKPPTECDDYYSCPEGTTCCCIYQYGSTCFGWGCCPLESATCCD 432

Query: 429 DHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRKVQKLGKLFVGA 480
           D YSCCP EYPVCDL  GTCR+SK S +GV+LLKRLPA     VQKLGK+ +G+
Sbjct: 433 DQYSCCPREYPVCDLAEGTCRMSKGSLIGVSLLKRLPAKHKGVVQKLGKMIIGS 486

[Close]

BLAST of CSPI07G07390 vs. ExPASy TrEMBL
Match: A0A6J1JKG6 (low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111486001 PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 2.0e-227
Identity = 390/485 (80.41%), Postives = 422/485 (87.01%), Query Frame = 0


Query: 1   MAISPIF----FAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIP-EIPHSDAHQRPD 60
           MAIS  F     AFLALF C S  S AS SS+FSIIDENAKHHL +    PH     R D
Sbjct: 1   MAISTTFSFSIVAFLALFLCFSSSSLASDSSSFSIIDENAKHHLDVQIGSPH-----RSD 60

Query: 61  EEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTN 120
           EEVAALYESWLVH+GKAYNA+GEKERRFEIFKDNLRFIDEHNRESR+YKVGL RFADL+N
Sbjct: 61  EEVAALYESWLVHNGKAYNALGEKERRFEIFKDNLRFIDEHNRESRSYKVGLNRFADLSN 120

Query: 121 EEYRARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWA 180
           EEYRA FL GR SRK RLSA KS RYA  LGDDLPD VDWRKKGAV  VKDQGQCGSCWA
Sbjct: 121 EEYRAMFLSGRLSRKNRLSATKSRRYAITLGDDLPDHVDWRKKGAVTDVKDQGQCGSCWA 180

Query: 181 FSSVAAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEED 240
           FS+VAAVEGINQI TGELI LSEQELVDCD+S+NMGCNGGLMDYAFQFII NGGIDTEED
Sbjct: 181 FSTVAAVEGINQITTGELISLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEED 240

Query: 241 YPYKGRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQ 300
           YPYKGRD  CDPNR+N+K VTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQ
Sbjct: 241 YPYKGRDGTCDPNRRNSKAVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQ 300

Query: 301 SGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKC 360
           SGVF G CGT+LDHGVVAVGYGT++G DYWIVRNSWGK+WGE+GYI+LERNV NITTGKC
Sbjct: 301 SGVFNGLCGTELDHGVVAVGYGTEDGKDYWIVRNSWGKNWGENGYIKLERNVGNITTGKC 360

Query: 361 GIAVQPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGC 420
           GIA++ SYPTK+G NPPKP+ SPPSPV PP +CDEYFSC+EG+TCCCIY++GSTCF WGC
Sbjct: 361 GIAIEASYPTKTGKNPPKPAPSPPSPVSPPNQCDEYFSCQEGTTCCCIYKYGSTCFGWGC 420

Query: 421 CPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRK-VQKLGK 480
           CPLESATCCDDHYSCCPHEYP+CDL+AGTC  SK S+ GV LLKR+PA   R  VQKLGK
Sbjct: 421 CPLESATCCDDHYSCCPHEYPICDLDAGTCLKSKGSTTGVKLLKRVPAKYNRGCVQKLGK 480

[Close]

BLAST of CSPI07G07390 vs. NCBI nr
Match: XP_004136967.1 (low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN43904.1 hypothetical protein Csa_017340 [Cucumis sativus])

HSP 1 Score: 989.2 bits (2556), Expect = 1.3e-284
Identity = 478/479 (99.79%), Postives = 479/479 (100.00%), Query Frame = 0


Query: 1   MAISPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAA 60
           MAISPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAA
Sbjct: 1   MAISPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAA 60

Query: 61  LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRA 120
           LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRA
Sbjct: 61  LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRA 120

Query: 121 RFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVA 180
           RFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVA
Sbjct: 121 RFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVA 180

Query: 181 AVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKG 240
           AVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKG
Sbjct: 181 AVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKG 240

Query: 241 RDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT 300
           RDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT
Sbjct: 241 RDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT 300

Query: 301 GRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQ 360
           GRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQ
Sbjct: 301 GRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQ 360

Query: 361 PSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLES 420
           PSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLES
Sbjct: 361 PSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLES 420

Query: 421 ATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRKVQKLGKLFVGA 480
           ATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQT+KVQKLGKLFVGA
Sbjct: 421 ATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTKKVQKLGKLFVGA 479

[Close]

BLAST of CSPI07G07390 vs. NCBI nr
Match: XP_008454976.1 (PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo])

HSP 1 Score: 939.9 bits (2428), Expect = 8.8e-270
Identity = 453/480 (94.38%), Postives = 465/480 (96.88%), Query Frame = 0


Query: 1   MAI-SPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVA 60
           MAI SPIFFAFLALF CLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQR DEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 ARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSV 180
           ARFLGGRFSRKP LSAAKSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQCGSCWAFS+V
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180

Query: 181 AAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYK 240
           AAVEGINQIVTGELI LSEQELVDCDKSFNMGCNGGLMDYAFQFII NGGIDT+EDYPYK
Sbjct: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240

Query: 241 GRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRD ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAV 360
           TGRCGT+LDHGVVAVGYGTDNGTDYWIVRNSWGKDWGE+GYIRLERNVAN TTGKCGIAV
Sbjct: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360

Query: 361 QPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLE 420
           +PSYP KSG+NPPKPSASPPSPV PPTECDEYFSC+EGSTCCCIYQ+GSTCFAWGCCPLE
Sbjct: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRKVQKLGKLFVGA 480
           SATCCDDHYSCCPHEYPVCDLEAGTCR SKDS MGVNLLKRLPA QT+++QKLGKLFVGA
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA 480

[Close]

BLAST of CSPI07G07390 vs. NCBI nr
Match: KAA0031322.1 (low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK06773.1 low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa])

HSP 1 Score: 928.7 bits (2399), Expect = 2.0e-266
Identity = 452/488 (92.62%), Postives = 464/488 (95.08%), Query Frame = 0


Query: 1   MAI-SPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVA 60
           MAI SPIFFAFLALF CLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQR DEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 ARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQ--------CG 180
           ARFLGGRFSRKP LSAAKSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQ         G
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQKFVMRSIFIG 180

Query: 181 SCWAFSSVAAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGID 240
           SCWAFS+VAAVEGINQIVTGELI LSEQELVDCDKSFNMGCNGGLMDYAFQFII NGGID
Sbjct: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240

Query: 241 TEEDYPYKGRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300
           T+EDYPYKGRD ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF
Sbjct: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300

Query: 301 QLYQSGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANIT 360
           QLYQSGVFTGRCGT+LDHGVVAVGYGTDNGTDYWIVRNSWGKDWGE+GYIRLERNVAN T
Sbjct: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360

Query: 361 TGKCGIAVQPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCF 420
           TGKCGIAV+PSYP KSG+NPPKPSASPPSPV PPTECDEYFSC+EGSTCCCIYQ+GSTCF
Sbjct: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420

Query: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRKVQK 480
           AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCR SKDS MGVNLLKRLPA QT+++QK
Sbjct: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQK 480

[Close]

BLAST of CSPI07G07390 vs. NCBI nr
Match: XP_038889032.1 (low-temperature-induced cysteine proteinase-like [Benincasa hispida])

HSP 1 Score: 888.3 bits (2294), Expect = 3.0e-254
Identity = 429/486 (88.27%), Postives = 454/486 (93.42%), Query Frame = 0


Query: 1   MAISPIF----FAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSD---AHQR 60
           MAIS  F     AFLALF C SPFSSASHSSTFSIIDENAKHH+GIP+I  SD   + QR
Sbjct: 1   MAISSTFSFLTLAFLALFLCFSPFSSASHSSTFSIIDENAKHHMGIPDIADSDHLRSPQR 60

Query: 61  PDEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADL 120
            D+EVAALYESWLVHH KAYNA+GEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADL
Sbjct: 61  TDDEVAALYESWLVHHRKAYNALGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADL 120

Query: 121 TNEEYRARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSC 180
           TNEEYRARFLGGRF  KPRLSAAKSGRYAAALGDDLPD VDWRKKGAVA VKDQGQCGSC
Sbjct: 121 TNEEYRARFLGGRFPGKPRLSAAKSGRYAAALGDDLPDHVDWRKKGAVAAVKDQGQCGSC 180

Query: 181 WAFSSVAAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTE 240
           WAFS+VAAVEGINQIVTGELI LSEQELVDCDKS+NMGCNGGLMDYAFQFII NGGIDTE
Sbjct: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTE 240

Query: 241 EDYPYKGRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300
           EDYPYKG DAACDPNRKNAKVVTIDG+EDVPENDESSLKKAVANQPVSVAIEAGGRAFQL
Sbjct: 241 EDYPYKGHDAACDPNRKNAKVVTIDGFEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300

Query: 301 YQSGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTG 360
           YQSGVFTGRCGT+LDHGVVAVGYGT+NGTDYWIVRNSWGK+WGESGYIRLERNVANITTG
Sbjct: 301 YQSGVFTGRCGTNLDHGVVAVGYGTENGTDYWIVRNSWGKNWGESGYIRLERNVANITTG 360

Query: 361 KCGIAVQPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAW 420
           KCGIAVQPSYP K+G+NPPKP+ASPPSPVKPPTECD+Y+SCEEG+TCCCIYQ+GSTCF W
Sbjct: 361 KCGIAVQPSYPIKTGSNPPKPAASPPSPVKPPTECDDYYSCEEGTTCCCIYQYGSTCFGW 420

Query: 421 GCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRKVQKLG 480
           GCCPLESATCCDDHYSCCPHEYP+CDLEAGTC + KDS+MGV LLKRLPA +T+ +QK G
Sbjct: 421 GCCPLESATCCDDHYSCCPHEYPICDLEAGTCLIDKDSTMGVKLLKRLPAKRTKGIQKFG 480

[Close]

BLAST of CSPI07G07390 vs. NCBI nr
Match: XP_022147795.1 (low-temperature-induced cysteine proteinase-like [Momordica charantia])

HSP 1 Score: 815.5 bits (2105), Expect = 2.5e-232
Identity = 388/474 (81.86%), Postives = 425/474 (89.66%), Query Frame = 0


Query: 9   AFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRP---DEEVAALYESW 68
           A LALF C   FSSAS SS+FSIIDENAKHHLG P+I  SDA   P    E+VAALY+SW
Sbjct: 13  ASLALFLCFLSFSSASDSSSFSIIDENAKHHLGFPDIAGSDAGTPPLRTQEQVAALYKSW 72

Query: 69  LVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRARFLGG 128
           LV HGKAYNA+GE+ERRFEIFKDNLRFIDEHNRE R+Y +GLTRFADLTNEEYRARFLGG
Sbjct: 73  LVKHGKAYNALGERERRFEIFKDNLRFIDEHNREPRSYTLGLTRFADLTNEEYRARFLGG 132

Query: 129 RFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVAAVEGI 188
           RFS KPR SAAK+GRYA++LG DLP+ VDWR+KGAV  VKDQGQCGSCWAFS++AAVEGI
Sbjct: 133 RFSPKPRPSAAKNGRYASSLGGDLPEHVDWREKGAVTAVKDQGQCGSCWAFSTIAAVEGI 192

Query: 189 NQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKGRDAAC 248
           NQIVTGELI LSEQELVDCDKS+NMGCNGGLMDYAFQFI+ NGGIDTEEDYPYKGRD  C
Sbjct: 193 NQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIVDNGGIDTEEDYPYKGRDGVC 252

Query: 249 DPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGT 308
           DPNR+NA VVTIDGYEDVPENDE +LKKAVA+QPVSVAIEAGGRAFQLYQSGVFTGRCGT
Sbjct: 253 DPNRRNANVVTIDGYEDVPENDEGALKKAVASQPVSVAIEAGGRAFQLYQSGVFTGRCGT 312

Query: 309 DLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQPSYPT 368
           DLDHGVVAVGYGT+NG DYWIVRNSWGK+WGE+GYIRLERNVANITTGKCGIA++PSYP 
Sbjct: 313 DLDHGVVAVGYGTENGLDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPV 372

Query: 369 KSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLESATCCD 428
           K+G NPPKP+ SPPSPVKPPTECD+Y+SC EG+TCCCIYQ+GSTCF WGCCPLESATCCD
Sbjct: 373 KTGKNPPKPAPSPPSPVKPPTECDDYYSCPEGTTCCCIYQYGSTCFGWGCCPLESATCCD 432

Query: 429 DHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRKVQKLGKLFVGA 480
           D YSCCP EYPVCDL  GTCR+SK S +GV+LLKRLPA     VQKLGK+ +G+
Sbjct: 433 DQYSCCPREYPVCDLAEGTCRMSKGSLIGVSLLKRLPAKHKGVVQKLGKMIIGS 486

[Close]

BLAST of CSPI07G07390 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 629.4 bits (1622), Expect = 2.4e-180
Identity = 299/453 (66.00%), Postives = 360/453 (79.47%), Query Frame = 0


Query: 13  LFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAALYESWLVHHGKA 72
           LF  +   SSA      SII  + KH +       S    R + EV ++YE+WLV HGKA
Sbjct: 11  LFLAMVAVSSA---VDMSIISYDEKHGV-------STTGGRSEAEVMSIYEAWLVKHGKA 70

Query: 73  --YNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRARFLGGRFSRK 132
              N++ EK+RRFEIFKDNLRF+DEHN ++ +Y++GLTRFADLTN+EYR+++LG +  +K
Sbjct: 71  QSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKK 130

Query: 133 PRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVAAVEGINQIVT 192
                  S RY A +GD+LP+ +DWRKKGAVA VKDQG CGSCWAFS++ AVEGINQIVT
Sbjct: 131 G--ERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVT 190

Query: 193 GELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKGRDAACDPNRK 252
           G+LI LSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDT++DYPYKG D  CD  RK
Sbjct: 191 GDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRK 250

Query: 253 NAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTDLDHG 312
           NAKVVTID YEDVP   E SLKKAVA+QP+S+AIEAGGRAFQLY SG+F G CGT LDHG
Sbjct: 251 NAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHG 310

Query: 313 VVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQPSYPTKSGAN 372
           VVAVGYGT+NG DYWIVRNSWGK WGESGY+R+ RN+A+ ++GKCGIA++PSYP K+G N
Sbjct: 311 VVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIAS-SSGKCGIAIEPSYPIKNGEN 370

Query: 373 PPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLESATCCDDHYSC 432
           PP P  SPPSP+KPPT+CD Y++C E +TCCC++++G  CFAWGCCPLE+ATCCDD+YSC
Sbjct: 371 PPNPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSC 430

Query: 433 CPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPA 464
           CPHEYPVCDL+ GTC +SK+S   V  LKR PA
Sbjct: 431 CPHEYPVCDLDQGTCLLSKNSPFSVKALKRKPA 450

[Close]

BLAST of CSPI07G07390 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 622.1 bits (1603), Expect = 3.8e-178
Identity = 304/462 (65.80%), Postives = 352/462 (76.19%), Query Frame = 0


Query: 8   FAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAALYESWLV 67
           F  L+    L      S++   SII  +  HH+       +    R D EV  +YE+W+V
Sbjct: 3   FLKLSPMILLLAMIGVSYAMDMSIISYDENHHI-------TTETSRSDSEVERIYEAWMV 62

Query: 68  HHGKA---YNAIG-EKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRARFL 127
            HGK     N +G EK++RFEIFKDNLRFIDEHN ++ +YK+GLTRFADLTNEEYR+ +L
Sbjct: 63  EHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYL 122

Query: 128 GGRFSRKPRLSAAK-SGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVAAV 187
           G     KP     K S RY A +GD LPD VDWRK+GAVA VKDQG CGSCWAFS++ AV
Sbjct: 123 GA----KPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAV 182

Query: 188 EGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKGRD 247
           EGIN+IVTG+LI LSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDTE DYPYK  D
Sbjct: 183 EGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAAD 242

Query: 248 AACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGR 307
             CD NRKNAKVVTID YEDVPEN E+SLKKA+A+QP+SVAIEAGGRAFQLY SGVF G 
Sbjct: 243 GRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGL 302

Query: 308 CGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQPS 367
           CGT+LDHGVVAVGYGT+NG DYWIVRNSWG  WGESGYI++ RN+    TGKCGIA++ S
Sbjct: 303 CGTELDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNI-EAPTGKCGIAMEAS 362

Query: 368 YPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLESAT 427
           YP K G NPP P  SPPSP+KPPT CD+YFSC E +TCCC+Y++G  CF WGCCPLE+AT
Sbjct: 363 YPIKKGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAAT 422

Query: 428 CCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAI 465
           CCDD+ SCCPHEYPVCD+  GTC +SK+S   V  LKR PAI
Sbjct: 423 CCDDNSSCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPAI 452

[Close]

BLAST of CSPI07G07390 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 557.4 bits (1435), Expect = 1.1e-158
Identity = 272/419 (64.92%), Postives = 322/419 (76.85%), Query Frame = 0


Query: 53  RPDEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNR-ESRTYKVGLTRFA 112
           R + E   +YE WLV + K YN +GEKERRFEIFKDNL+F++EH+   +RTY+VGLTRFA
Sbjct: 34  RNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFA 93

Query: 113 DLTNEEYRARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCG 172
           DLTN+E+RA +L  +  R       K  +Y   +GD LPD +DWR KGAV  VKDQG CG
Sbjct: 94  DLTNDEFRAIYLRSKMER--TRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCG 153

Query: 173 SCWAFSSVAAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGID 232
           SCWAFS++ AVEGINQI TGELI LSEQELVDCD S+N GC GGLMDYAF+FII NGGID
Sbjct: 154 SCWAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGID 213

Query: 233 TEEDYPYKGRDA-ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRA 292
           TEEDYPY   D   C+ ++KN +VVTIDGYEDVP+NDE SLKKA+ANQP+SVAIEAGGRA
Sbjct: 214 TEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRA 273

Query: 293 FQLYQSGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANI 352
           FQLY SGVFTG CGT LDHGVVAVGYG++ G DYWIVRNSWG +WGESGY +LERN+   
Sbjct: 274 FQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKE- 333

Query: 353 TTGKCGIAVQPSYPTK-SGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGST 412
           ++GKCG+A+  SYPTK SG+NPPKP A  PSPV     CD+  +C   STCCC+Y++   
Sbjct: 334 SSGKCGVAMMASYPTKSSGSNPPKPPA--PSPV----VCDKSNTCPAKSTCCCLYEYNGK 393

Query: 413 CFAWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTRK 469
           C++WGCCP ESATCCDD  SCCP  YPVCDL+A TCR+  +S + +  L R PAI T K
Sbjct: 394 CYSWGCCPYESATCCDDGSSCCPQSYPVCDLKANTCRMKGNSPLSIKALTRGPAIATTK 443

[Close]

BLAST of CSPI07G07390 vs. TAIR 10
Match: AT4G36880.1 (cysteine proteinase1 )

HSP 1 Score: 438.3 bits (1126), Expect = 7.7e-123
Identity = 216/342 (63.16%), Postives = 264/342 (77.19%), Query Frame = 0


Query: 39  HLGIPEIPHSDAHQRPDEEVAALYESWLVHHGKAYN----AIGEKERRFEIFKDNLRFID 98
           HL +P    SD   R DEEV ++Y  W   HGK  N     I ++++RF IFKDNLRFID
Sbjct: 30  HLQLP----SDGKWRTDEEVRSIYLQWSAEHGKTNNNNNGIINDQDKRFNIFKDNLRFID 89

Query: 99  EHNRESR--TYKVGLTRFADLTNEEYRARFLGGRFSRKPRLSAAK--SGRYAAAL-GDDL 158
            HN  ++  TYK+GLT+F DLTN+EYR  +LG R     R++ AK  + +Y+AA+ G ++
Sbjct: 90  LHNENNKNATYKLGLTKFTDLTNDEYRKLYLGARTEPARRIAKAKNVNQKYSAAVNGKEV 149

Query: 159 PDDVDWRKKGAVATVKDQGQCGSCWAFSSVAAVEGINQIVTGELIPLSEQELVDCDKSFN 218
           P+ VDWR+KGAV  +KDQG CGSCWAFS+ AAVEGIN+IVTGELI LSEQELVDCDKS+N
Sbjct: 150 PETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGINKIVTGELISLSEQELVDCDKSYN 209

Query: 219 MGCNGGLMDYAFQFIIGNGGIDTEEDYPYKGRDAACDPNRKNAKVVTIDGYEDVPENDES 278
            GCNGGLMDYAFQFI+ NGG++TE+DYPY+G    C+   KN++VV+IDGYEDVP  DE+
Sbjct: 210 QGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKCNSFLKNSRVVSIDGYEDVPTKDET 269

Query: 279 SLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRN 338
           +LKKA++ QPVSVAIEAGGR FQ YQSG+FTG CGT+LDH VVAVGYG++NG DYWIVRN
Sbjct: 270 ALKKAISYQPVSVAIEAGGRIFQHYQSGIFTGSCGTNLDHAVVAVGYGSENGVDYWIVRN 329

Query: 339 SWGKDWGESGYIRLERNVANITTGKCGIAVQPSYPTKSGANP 372
           SWG  WGE GYIR+ERN+A   +GKCGIAV+ SYP K   NP
Sbjct: 330 SWGPRWGEEGYIRMERNLAASKSGKCGIAVEASYPVKYSPNP 367

[Close]

BLAST of CSPI07G07390 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 431.4 bits (1108), Expect = 9.4e-121
Identity = 207/391 (52.94%), Postives = 264/391 (67.52%), Query Frame = 0


Query: 56  EEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNR-ESRTYKVGLTRFADLT 115
           ++++ L++ W   HGK Y +  E+++R +IFKDN  F+ +HN   + TY + L  FADLT
Sbjct: 26  DDISELFDDWCQKHGKTYGSEEERQQRIQIFKDNHDFVTQHNLITNATYSLSLNAFADLT 85

Query: 116 NEEYRARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCW 175
           + E++A  LG   S    + A+K      ++   +PD VDWRKKGAV  VKDQG CG+CW
Sbjct: 86  HHEFKASRLGLSVSAPSVIMASKGQSLGGSV--KVPDSVDWRKKGAVTNVKDQGSCGACW 145

Query: 176 AFSSVAAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEE 235
           +FS+  A+EGINQIVTG+LI LSEQEL+DCDKS+N GCNGGLMDYAF+F+I N GIDTE+
Sbjct: 146 SFSATGAMEGINQIVTGDLISLSEQELIDCDKSYNAGCNGGLMDYAFEFVIKNHGIDTEK 205

Query: 236 DYPYKGRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLY 295
           DYPY+ RD  C  ++   KVVTID Y  V  NDE +L +AVA QPVSV I    RAFQLY
Sbjct: 206 DYPYQERDGTCKKDKLKQKVVTIDSYAGVKSNDEKALMEAVAAQPVSVGICGSERAFQLY 265

Query: 296 QSGVFTGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGK 355
            SG+F+G C T LDH V+ VGYG+ NG DYWIV+NSWGK WG  G++ ++RN  N + G 
Sbjct: 266 SSGIFSGPCSTSLDHAVLIVGYGSQNGVDYWIVKNSWGKSWGMDGFMHMQRNTEN-SDGV 325

Query: 356 CGIAVQPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWG 415
           CGI +  SYP K+  NPP P  SPP     PT+C+ +  C  G TCCC  +    CF+W 
Sbjct: 326 CGINMLASYPIKTHPNPPPP--SPPG----PTKCNLFTYCSSGETCCCARELFGLCFSWK 385

Query: 416 CCPLESATCCDDHYSCCPHEYPVCDLEAGTC 446
           CC +ESA CC D   CCPH+YPVCD     C
Sbjct: 386 CCEIESAVCCKDGRHCCPHDYPVCDTTRSLC 407

The following BLAST results are available for this feature:

BLAST of CSPI07G07390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43297	3.3e-179	66.00	Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1	[more]
Q9FMH8	5.3e-177	65.80	Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S...	[more]
P25776	2.9e-175	68.77	Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=...	[more]
Q9LT78	1.6e-157	64.92	Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 S...	[more]
P25777	4.4e-155	60.86	Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1...	[more]

BLAST of CSPI07G07390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4N3	6.1e-285	99.79	Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_7G073400 PE=3 SV=1	[more]
A0A1S3BZW8	4.2e-270	94.38	low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A5A7SJU5	9.8e-267	92.62	Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A6J1D149	1.2e-232	81.86	low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1JKG6	2.0e-227	80.41	low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=...	[more]

BLAST of CSPI07G07390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136967.1	1.3e-284	99.79	low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN43904.1 hypoth...	[more]
XP_008454976.1	8.8e-270	94.38	PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo]	[more]
KAA0031322.1	2.0e-266	92.62	low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK...	[more]
XP_038889032.1	3.0e-254	88.27	low-temperature-induced cysteine proteinase-like [Benincasa hispida]	[more]
XP_022147795.1	2.5e-232	81.86	low-temperature-induced cysteine proteinase-like [Momordica charantia]	[more]

BLAST of CSPI07G07390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47128.1	2.4e-180	66.00	Granulin repeat cysteine protease family protein	[more]
AT5G43060.1	3.8e-178	65.80	Granulin repeat cysteine protease family protein	[more]
AT3G19390.1	1.1e-158	64.92	Granulin repeat cysteine protease family protein	[more]
AT4G36880.1	7.7e-123	63.16	cysteine proteinase1	[more]
AT1G09850.1	9.4e-121	52.94	xylem bark cysteine peptidase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 167..182 score: 65.45 coord: 324..330 score: 73.06 coord: 309..319 score: 57.41
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 149..365 e-value: 2.6E-126 score: 435.6
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 149..364 e-value: 6.4E-84 score: 281.4
IPR000118	Granulin	SMART	SM00277	GRAN_2	coord: 388..445 e-value: 3.2E-26 score: 103.1
IPR000118	Granulin	PFAM	PF00396	Granulin	coord: 399..445 e-value: 3.0E-10 score: 40.3
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 62..118 e-value: 7.7E-26 score: 101.8
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 62..118 e-value: 5.9E-18 score: 65.1
IPR037277	Granulin superfamily	GENE3D	2.10.25.160	Granulin	coord: 385..448 e-value: 2.7E-13 score: 52.0
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 44..367 e-value: 5.8E-121 score: 405.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 367..385
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 365..385
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 43..394
None	No IPR available	PANTHER	PTHR12411:SF749	CYSTEINE PROTEASE	coord: 43..394
None	No IPR available	SUPERFAMILY	57277	Granulin repeat	coord: 385..419
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 167..178
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 324..343
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 307..317
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 150..364 e-value: 1.28983E-110 score: 324.192
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 54..365

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G07390.1	CSPI07G07390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity