CSPI07G05150 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G05150
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description2-(3-amino-3-carboxypropyl)histidine synthase subunit 1
LocationChr7: 3819542 .. 3823954 (-)
RNA-Seq ExpressionCSPI07G05150
SyntenyCSPI07G05150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGTTCATAGTTCATAGGGTTCCTAAATTTTGGAAATTCGCCTTTTAGTTTTTCTCATACCAAAAATGAAGTCGGAGCTCCAAAACCCATCATCAGAGCTTGCCGTTCTCTCGCAAAGCACCGAGGACAAGCCGCCGCCAAGATCCAGGCCGCCTCCGAAGCGATTTGTGAAGAATCAGATTCCCGATTCCATCCTTAACGATCCTGCCTTGAATGCCGCCATCTCTCTCCTTCCTTCCAACTACAACTTCGAGGTTCACAAGTGCGTATGGCGAATTCTCTCCTCCTCCGCCTCCCGTGTTGCCCTTCAGCTCCCCGAAGGTCTTCTCATGTACTCTCTTATCCTCTCCGATATCCTCACCACTTTCGGCAAAGCCTCCCACTGCTTCGTTCTTGGCGATGTCACTTTTGGCGCTTGTTGTGTTGATGACCTTTCCGCCTCCGCTCTCCATGCCGATCTCCTCATCCATTACGGCCACAGTTGCCTCGTCCCTATCGATTCCACTACCATTCCCTGCCTCTATGTTTTTGTAGACATTGCTATTGATGTGCCTCGTCTTGTTAATACTGTTCGTCTCAATGTTGATTGCCTTGATTCTACAAATTTGGTGCTTGCTGGGACAATTCAATTCGCCTCTGCTATTAGAGCTTCGAAGATGGAGCTAGAGAGACATGGCATCAGGGTTTTAATTCCTCAGTCCAGGCCTTTGTCTGCTGGGGAAGTTCTGGGCTGTACGGCTCCTAAAGTTTCTACTGCTGCGTTCGGTAATAATGGGGAGACTGTTGCGATTTTTGTGGCCGATGGGAGGTTTCATTTGGAAGCTTTCATGATTGCGAATCCTGGAATTAGGGTTTTCCGGTATGATCCGTATATTGGTAAGTTATTGCTTGAGGAGTATGATCATGAAGGAATGAAGGCGTCGAGAAAAGGCGCGATTCTGAAGGCGAAGGAAGCTACAAATTGGGGTATTGTGTTGGGAACTTTAGGAAGGCAAGGGAACCCTAAAATATTGGAGAGATTGGAAGAGAAGATGAAGTCTAAAGGATTTGATTATACTGTTGTATTAATGTCTGAAATCAGTCCTTATAGAGTGGCTTTGTTTGAGGATTCGGTGGATGCATGGATTCAGATAGCTTGTCCCCGTCTTTCAATTGACTGGGGAGATGCTTTTGCTAAGCCTCTTCTTACTCCATTTGAGGCTGAGGTAGCTCTTGGACTAATTCCTGGTTGGTGGGAGAGGACGAGCTCAGCTAAATTGAGTTGTAATGATTGTCGGGAATGTGGATGTAATGGCGATGAAACAAATAAGGCGGAAGCTGAAGGAGAATATCCTATGGATTATTATGCTCAGGATGGTGGTGAGTGGAACTCTTCCTATGTAAAGAAGAAAGTTTCTCGCCCCGTCCGGAGAGATGTTGTTTCCTGTATCAGTGACAAAGCCATTTCTTTACATTAGCCTCTTCAAATAAGGTATACAAGATTGCTTAATGTTTTATATGTTAGAACTAGAAGTGCTGCTTCCTCTGAAATTTTTTATTGTACATATAAGCATACAACTCTATTATCTACAGCTTTATCAAATATTGGTCTGACACTGCATTTTAATTTGAAGATTTTTAGATGGCAATGTTGCTTTCTGAAATCATTCTTATATCAATCATGTGTATGAATTTGCTTTAGTATGGTTGACTGTTGGCTTGTACAATTTTATTTTGTTTGGATTTCTTTGGAAATGTAGTTTAACATTTTGGATTATTAGTTATTTCAAGTTTAATGGGGAGTTAGGTGAATTTTGGCCCATCTATGGACATGTGTTCTGTGATTCAACGTTGAGTGCAATGCAATACATGAATTGCTCTGTTCTATATGTTACTGGGAGCATTTTCTGGCCTAATACTTCATTGGTTCTGCCTTTTGTCGTATTATCTTTCAACTTTTCTAAATCTGCATGCCATTTTCTTTTATGGCGTCAAAAGTGTTTGGACAATATAATTCAAATGATTTGTCTGACAGAAAATCAAATTCTTCGGTAGATGAAGCAACTCCCTTATAGTTCAAGACATGATTAGGCTTTACAAGTTCTGGTGGACTAAATTACTCCTCTTGGAGCTGTGGCTACTTATTACCTCTTCAATAGAAAACGAAATAGATTTCATGATGATGATGGACACACACAACATTCTGGTGAGTCTGATACTTTTGTGCACTCATGGCTTCGGAGTGATGATATGGTGATTTCTTGAAATCTAAATTTGAATTTTATGGCAATTTTTTTCTCTCTTTTTTATGAATAACTAAAAGATGAAAGGGCAATTACAAAAGTACTTTGTCATCGAAGCCCATAGAGAAACACATAACCTCACAAAAGACCTTACATTGCCCGAAGATCTTGTTAGCTCTTCAAAAATTCTTTTGTTCCTCTCTCAAAAGTCCCCACAAGGTCGCTCACATTCCCAAGACTTTCTTTTTTCGTGAAAGATTGGATGGAGAAGGAATTCCTCAAACATCTTTTGCAACCCCTATGCCTAGCCAAACCAAGACCAAAGGATGCAACAGCACGACCCCCCATTATGGGTGCCTTCCTCATCACCCTGCTCAACTTGTTAACTCTTCCGTGGAGCTCCAACCAAATGAAGAGCTTAACTTTAAATTACATACCCGGTTCTTATGGTTTGGATGAAGTTCTAATTTACTCCTTAACTAGAAAAACTACTAACCTTGACACATCATGACGATGTAGAAGAGATTAGGAAGCATTCAAAATAAAACTACAAGTTTATCAAGACTAAATTGATAGAAGAAGAAAATAGGTTGTGCCTGCTGAAATCTTCTCCATCTCCTCCATTCTTTTCTTGTTTTTCAAATCCTAAGATCTGACGTGAATATATAGGAAATTGGGGTGGCGTTGAGGCATCTTTTCACAGGGATGCACATGCACGCACAAAAAGGTGATGGCACCATGACACTACCACAAAATCATGCCTTATATCTCTAGCATTGCACGACAATGGGGCGCTGCACTGTGGGGCATTTTTGTCATTTGGCTCCTTTTGTTGCTATTTGTTCTATTTTTAGCCCAACTTGTACCAGGACGTCCCGAGAGTCTCCTTTGCTCTATTTCCTTTCTAAGAACACATTTAAGCATTAAATTCATAAGAACAAGCTTGATTTAGGCAGCTCCTTTTTTTTAAAAAAAAAGAACAGCTCTCTTAGAGAGCTATCATCCACAAATGATTTCTTATCGAAGATAAATGGTATGGAGGAAGAAATACAGAGTAGAGGGGTCTTTATAATTCTAATTTATTCGACTAGCTCTCATTTAGGTAGTTTTTAAAAAGTTAAAGATTGTTGGGGAAGGGATGGACAACTAGATTGCTTTTCTTGGCACACTGCATATTTCGTGTAATGTGATCTTTATCTCATTACTATCAGGCTGAATACATTATATTTTTCCCGATGGAACTAGTGTGCATACCCCATCTCTTGTGTATCCAACCCTTCTCATTATCCTAGTATTTTCTCATGTTGTAAAAAACATCAGCATAATGTTATAGTTGTCAACCATTGTGATTAGTCAAATTAGTGCGTGAACTAATACTAATTTCCTTGATACAGACGGAGAAAGTCATGCACATATTGTGGTGCATCTGTGCACGTGGTTGATAAGTATTATAAAGTTGCATGGATATCCTTCTGGTCAAACAGTGAAACAATTCCTGAGGCATCCTTTCTCTCACTTTGAGATTCTCTCACTTTGAGAGTAGTCGAGATTTTCGTTTCACAGTAGTTAAAAATGTTTTGGAAATTCTAGCGTCTGCTGCCATGGCTGATTGAAAGAGTGTGTTCCACCATGAGTTTTGTTCCACCGAGAGTTGTGTTCCATCTCTCAGCCAAAGCAGTGTCAGAACTCAATCTTAAGTATAAGTTGCACATTTTTCGACATCTTACTATATTGTCAAATCATGATAACATTTTTGTTGCACATGTTTGTGAAGCTCGAATATCATACTGAAGAGTATTCTTCTTTTGCTAAGTCTCAGCATCCATATGTTGCTGTTTTCCAACCAAGTCTTCTTTATTTGTGGACTTTTCTTGTGCTGGCTCATGTTCTAAATCAATTGAGTTTGGAGATGAAAGTCGTTGGCAAGAGTAGGGAAGATGGGTGCATTTAATGTTTACATGACTGCTTGAAATTCTTTTGGGACATAAATAACTTAGGTCTTAGAATTGCTTACTCGTTGCCAAGGGTTTTTGTTTTGGCTCAGTTTATTTTATTTTTCTGTGTTTCTTTTGTCCATAAACAAATGAAGTTGAAATTATGACGTGGGACATTGTTGTATGCTTGCTTTGACAAGCAAAACAAAAGTTGGCCACCTTTTACATCGTAAACAATATGAAAAGATAAATTAGT

mRNA sequence

GTGGTTCATAGTTCATAGGGTTCCTAAATTTTGGAAATTCGCCTTTTAGTTTTTCTCATACCAAAAATGAAGTCGGAGCTCCAAAACCCATCATCAGAGCTTGCCGTTCTCTCGCAAAGCACCGAGGACAAGCCGCCGCCAAGATCCAGGCCGCCTCCGAAGCGATTTGTGAAGAATCAGATTCCCGATTCCATCCTTAACGATCCTGCCTTGAATGCCGCCATCTCTCTCCTTCCTTCCAACTACAACTTCGAGGTTCACAAGTGCGTATGGCGAATTCTCTCCTCCTCCGCCTCCCGTGTTGCCCTTCAGCTCCCCGAAGGTCTTCTCATGTACTCTCTTATCCTCTCCGATATCCTCACCACTTTCGGCAAAGCCTCCCACTGCTTCGTTCTTGGCGATGTCACTTTTGGCGCTTGTTGTGTTGATGACCTTTCCGCCTCCGCTCTCCATGCCGATCTCCTCATCCATTACGGCCACAGTTGCCTCGTCCCTATCGATTCCACTACCATTCCCTGCCTCTATGTTTTTGTAGACATTGCTATTGATGTGCCTCGTCTTGTTAATACTGTTCGTCTCAATGTTGATTGCCTTGATTCTACAAATTTGGTGCTTGCTGGGACAATTCAATTCGCCTCTGCTATTAGAGCTTCGAAGATGGAGCTAGAGAGACATGGCATCAGGGTTTTAATTCCTCAGTCCAGGCCTTTGTCTGCTGGGGAAGTTCTGGGCTGTACGGCTCCTAAAGTTTCTACTGCTGCGTTCGGTAATAATGGGGAGACTGTTGCGATTTTTGTGGCCGATGGGAGGTTTCATTTGGAAGCTTTCATGATTGCGAATCCTGGAATTAGGGTTTTCCGGTATGATCCGTATATTGGTAAGTTATTGCTTGAGGAGTATGATCATGAAGGAATGAAGGCGTCGAGAAAAGGCGCGATTCTGAAGGCGAAGGAAGCTACAAATTGGGGTATTGTGTTGGGAACTTTAGGAAGGCAAGGGAACCCTAAAATATTGGAGAGATTGGAAGAGAAGATGAAGTCTAAAGGATTTGATTATACTGTTGTATTAATGTCTGAAATCAGTCCTTATAGAGTGGCTTTGTTTGAGGATTCGGTGGATGCATGGATTCAGATAGCTTGTCCCCGTCTTTCAATTGACTGGGGAGATGCTTTTGCTAAGCCTCTTCTTACTCCATTTGAGGCTGAGGTAGCTCTTGGACTAATTCCTGGTTGGTGGGAGAGGACGAGCTCAGCTAAATTGAGTTGTAATGATTGTCGGGAATGTGGATGTAATGGCGATGAAACAAATAAGGCGGAAGCTGAAGGAGAATATCCTATGGATTATTATGCTCAGGATGGTGGTGAGTGGAACTCTTCCTATGTAAAGAAGAAAGTTTCTCGCCCCGTCCGGAGAGATGTTGTTTCCTGTATCAGTGACAAAGCCATTTCTTTACATTAGCCTCTTCAAATAAGAAAATCAAATTCTTCGGTAGATGAAGCAACTCCCTTATAGTTCAAGACATGATTAGGCTTTACAAGTTCTGGTGGACTAAATTACTCCTCTTGGAGCTGTGGCTACTTATTACCTCTTCAATAGAAAACGAAATAGATTTCATGATGATGATGGACACACACAACATTCTGACGGAGAAAGTCATGCACATATTGTGGTGCATCTGTGCACGTGGTTGATAAGTATTATAAAGTTGCATGGATATCCTTCTGGTCAAACAGTGAAACAATTCCTGAGGCATCCTTTCTCTCACTTTGAGATTCTCTCACTTTGAGAGTAGTCGAGATTTTCGTTTCACAGTAGTTAAAAATGTTTTGGAAATTCTAGCGTCTGCTGCCATGGCTGATTGAAAGAGTGTGTTCCACCATGAGTTTTGTTCCACCGAGAGTTGTGTTCCATCTCTCAGCCAAAGCAGTGTCAGAACTCAATCTTAAGTATAAGTTGCACATTTTTCGACATCTTACTATATTGTCAAATCATGATAACATTTTTGTTGCACATGTTTGTGAAGCTCGAATATCATACTGAAGAGTATTCTTCTTTTGCTAAGTCTCAGCATCCATATGTTGCTGTTTTCCAACCAAGTCTTCTTTATTTGTGGACTTTTCTTGTGCTGGCTCATGTTCTAAATCAATTGAGTTTGGAGATGAAAGTCGTTGGCAAGAGTAGGGAAGATGGGTGCATTTAATGTTTACATGACTGCTTGAAATTCTTTTGGGACATAAATAACTTAGGTCTTAGAATTGCTTACTCGTTGCCAAGGGTTTTTGTTTTGGCTCAGTTTATTTTATTTTTCTGTGTTTCTTTTGTCCATAAACAAATGAAGTTGAAATTATGACGTGGGACATTGTTGTATGCTTGCTTTGACAAGCAAAACAAAAGTTGGCCACCTTTTACATCGTAAACAATATGAAAAGATAAATTAGT

Coding sequence (CDS)

ATGAAGTCGGAGCTCCAAAACCCATCATCAGAGCTTGCCGTTCTCTCGCAAAGCACCGAGGACAAGCCGCCGCCAAGATCCAGGCCGCCTCCGAAGCGATTTGTGAAGAATCAGATTCCCGATTCCATCCTTAACGATCCTGCCTTGAATGCCGCCATCTCTCTCCTTCCTTCCAACTACAACTTCGAGGTTCACAAGTGCGTATGGCGAATTCTCTCCTCCTCCGCCTCCCGTGTTGCCCTTCAGCTCCCCGAAGGTCTTCTCATGTACTCTCTTATCCTCTCCGATATCCTCACCACTTTCGGCAAAGCCTCCCACTGCTTCGTTCTTGGCGATGTCACTTTTGGCGCTTGTTGTGTTGATGACCTTTCCGCCTCCGCTCTCCATGCCGATCTCCTCATCCATTACGGCCACAGTTGCCTCGTCCCTATCGATTCCACTACCATTCCCTGCCTCTATGTTTTTGTAGACATTGCTATTGATGTGCCTCGTCTTGTTAATACTGTTCGTCTCAATGTTGATTGCCTTGATTCTACAAATTTGGTGCTTGCTGGGACAATTCAATTCGCCTCTGCTATTAGAGCTTCGAAGATGGAGCTAGAGAGACATGGCATCAGGGTTTTAATTCCTCAGTCCAGGCCTTTGTCTGCTGGGGAAGTTCTGGGCTGTACGGCTCCTAAAGTTTCTACTGCTGCGTTCGGTAATAATGGGGAGACTGTTGCGATTTTTGTGGCCGATGGGAGGTTTCATTTGGAAGCTTTCATGATTGCGAATCCTGGAATTAGGGTTTTCCGGTATGATCCGTATATTGGTAAGTTATTGCTTGAGGAGTATGATCATGAAGGAATGAAGGCGTCGAGAAAAGGCGCGATTCTGAAGGCGAAGGAAGCTACAAATTGGGGTATTGTGTTGGGAACTTTAGGAAGGCAAGGGAACCCTAAAATATTGGAGAGATTGGAAGAGAAGATGAAGTCTAAAGGATTTGATTATACTGTTGTATTAATGTCTGAAATCAGTCCTTATAGAGTGGCTTTGTTTGAGGATTCGGTGGATGCATGGATTCAGATAGCTTGTCCCCGTCTTTCAATTGACTGGGGAGATGCTTTTGCTAAGCCTCTTCTTACTCCATTTGAGGCTGAGGTAGCTCTTGGACTAATTCCTGGTTGGTGGGAGAGGACGAGCTCAGCTAAATTGAGTTGTAATGATTGTCGGGAATGTGGATGTAATGGCGATGAAACAAATAAGGCGGAAGCTGAAGGAGAATATCCTATGGATTATTATGCTCAGGATGGTGGTGAGTGGAACTCTTCCTATGTAAAGAAGAAAGTTTCTCGCCCCGTCCGGAGAGATGTTGTTTCCTGTATCAGTGACAAAGCCATTTCTTTACATTAG

Protein sequence

MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNYNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCVDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTNLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETVAIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNWGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPRLSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAEGEYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCISDKAISLH*
Homology
BLAST of CSPI07G05150 vs. ExPASy Swiss-Prot
Match: Q54PW5 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Dictyostelium discoideum OX=44689 GN=dph1 PE=3 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 4.7e-106
Identity = 193/401 (48.13%), Postives = 265/401 (66.08%), Query Frame = 0

Query: 38  QIPDSILNDPALNAAISLLPSNYNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDI 97
           QIPD I+ND  LN A+ +LPSNYNFE+ K +WRI  +SA RVALQ PEGLLMYS I+SDI
Sbjct: 103 QIPDEIMNDKELNLAVKILPSNYNFEIFKTIWRIKQASAKRVALQFPEGLLMYSCIISDI 162

Query: 98  LTTFGKASHCFVLGDVTFGACCVDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVD 157
           +  F       ++GDVT+GACCVDD +A +L AD ++HYGHSCL+PID + I  LYVFVD
Sbjct: 163 IEKFASV-ETIIMGDVTYGACCVDDYTARSLGADFMVHYGHSCLIPIDVSEIKMLYVFVD 222

Query: 158 IAIDVPRLVNTVRLNVDCLDSTNLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSA 217
           I  D+   + T++ N     +  L++  TIQF++++++S+  L  +   + IPQ +PLS 
Sbjct: 223 IQFDLQHFIETLKFNFK--QTQKLIMVSTIQFSASLQSSREPLSEYFSNIFIPQEKPLSP 282

Query: 218 GEVLGCTAPKVSTAAFGNNGET--VAIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLL 277
           GE+LGCT+PK+   +   + E   + I++ DGRFHLE+ MI+NP ++ +RYDPY     L
Sbjct: 283 GEILGCTSPKIKFTSPDGDEENNEIVIYLGDGRFHLESIMISNPHVKSYRYDPYSKVFSL 342

Query: 278 EEYDHEGMKASRKGAILKAKEATNWGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLM 337
           E+YD + M   R+ AI  A +AT +GI+LGTLGRQG+PKIL+ LE+ +KS G  YT VL+
Sbjct: 343 EKYDFQEMYKIRRDAIETASKATKFGIILGTLGRQGSPKILDHLEQLLKSNGKHYTTVLL 402

Query: 338 SEISPYRVALFEDSVDAWIQIACPRLSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSS 397
           SEI P ++ +F D +++WIQIACPRLSIDWG AF  PLL P+EAEV LG I  W      
Sbjct: 403 SEIFPAKLDMFSD-IESWIQIACPRLSIDWGYAFTTPLLNPYEAEVCLGGI-NW------ 462

Query: 398 AKLSCNDCRECGCNGDETNKAEAEGEYPMDYYAQDGGEWNS 437
                                  +  YPMD+Y+++GG+W +
Sbjct: 463 -----------------------QSVYPMDFYSKEGGKWTN 469

BLAST of CSPI07G05150 vs. ExPASy Swiss-Prot
Match: Q4IQ72 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=DPH1 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 1.9e-102
Identity = 190/395 (48.10%), Postives = 256/395 (64.81%), Query Frame = 0

Query: 37  NQIPDSILNDPALNAAISLLPSNYNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSD 96
           NQ+P  IL +P L AAISLLP+NYNFE+ K + RI +S + RVALQ+PEGLL+++  +SD
Sbjct: 92  NQVPKEILENPDLKAAISLLPANYNFEIPKTIHRIQTSGSKRVALQMPEGLLLFATTISD 151

Query: 97  ILTTFGKASHCFVLGDVTFGACCVDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFV 156
           ILT F       ++GDVT+GACC+DD +A AL  DLL+HY HSCL+P+D TTI  LYVFV
Sbjct: 152 ILTQFCPGIETLIMGDVTYGACCIDDYTARALGCDLLVHYAHSCLIPVDVTTIKTLYVFV 211

Query: 157 DIAIDVPRLVNTVRLNVDCLDSTNLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLS 216
           DI+ID   L+ ++  N        + + GTIQF + I   K  LER G RVL+PQ  PLS
Sbjct: 212 DISIDATHLLASLEHNF--ASGKTIAVVGTIQFNATIHGVKSSLERGGFRVLVPQIAPLS 271

Query: 217 AGEVLGCTAPKVSTAAFGNNGETVAIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLE 276
            GE+LGCT+P+++     ++   + +++ DGRFHLE+ MI NP I  +RYDPY  KL  E
Sbjct: 272 KGEILGCTSPRLTE----DDKIDLILYLGDGRFHLESIMIHNPTIPAYRYDPYSRKLTRE 331

Query: 277 EYDHEGMKASRKGAILKAKEATNWGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMS 336
            Y HE M++ R+ AI  A+ A  WG++LG LGRQGNP  L  +E+++K++G     +L+S
Sbjct: 332 TYGHEEMQSLRRTAIHSARSARKWGLILGALGRQGNPHTLGLIEKELKARGIPIVHLLLS 391

Query: 337 EISPYRVALFEDSVDAWIQIACPRLSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSA 396
           EI P ++AL  D ++ W+Q+ACPRLSIDWG AF++PLLTP+EA VALG    W       
Sbjct: 392 EIFPGKLALMSD-IECWVQVACPRLSIDWGYAFSRPLLTPYEALVALGEKKDW------- 451

Query: 397 KLSCNDCRECGCNGDETNKAEAEGEYPMDYYAQDG 432
                                 +G YPMDYY +DG
Sbjct: 452 ---------------------GDGVYPMDYYGKDG 451

BLAST of CSPI07G05150 vs. ExPASy Swiss-Prot
Match: O59713 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dph1 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 9.2e-102
Identity = 188/363 (51.79%), Postives = 245/363 (67.49%), Query Frame = 0

Query: 35  VKNQIPDSILNDPALNAAISLLPSNYNFEVHKCVWRILSSSASRVALQLPEGLLMYSLIL 94
           V  QIP+ ILND A+N AI LLP NYNFE+HK +W I    A RVALQLPEGLLM+  IL
Sbjct: 48  VATQIPEDILNDKAINEAIKLLPQNYNFEIHKTIWHIRLRKAKRVALQLPEGLLMFGCIL 107

Query: 95  SDILTTFGKASHCFVLGDVTFGACCVDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYV 154
           SDI   F +     V+GDVT+GACC+DD +A AL  D L+HYGHSCL+P+D T I  LYV
Sbjct: 108 SDIFEQFCQV-ETIVMGDVTYGACCIDDFTARALDCDFLVHYGHSCLIPVDQTPIKVLYV 167

Query: 155 FVDIAIDVPRLVNTVRLNVDCLDSTNLVLAGTIQFASAIRASKMELE------RHGIRVL 214
           FVDI ID+  +V++++ N+    ++ L L GTIQF  ++ + K  L+      + G  V+
Sbjct: 168 FVDIKIDLQHVVSSLKHNLP--SNSRLALVGTIQFVGSLNSIKDALQIQDEDGKGGFYVV 227

Query: 215 IPQSRPLSAGEVLGCTAPKVSTAAFGNNGETVAIFVADGRFHLEAFMIANPGIRVFRYDP 274
           IPQ++PLS GE LGCT+P +   +         I++ DGRFHLE+ MIANP +  +RYDP
Sbjct: 228 IPQAKPLSPGEALGCTSPYIEKGSV-----DALIYIGDGRFHLESVMIANPDLPAYRYDP 287

Query: 275 YIGKLLLEEYDHEGMKASRKGAILKAKEATNWGIVLGTLGRQGNPKILERLEEKMKSKGF 334
           Y  KL +E Y HE MK+ R  A+ KA+ A  +G++ GTLGRQG+PK+LE L+  ++    
Sbjct: 288 YSHKLSIESYAHEEMKSIRYSAVEKARTAKKFGLIQGTLGRQGSPKVLENLKNTLRKNNK 347

Query: 335 DYTVVLMSEISPYRVALFEDSVDAWIQIACPRLSIDWGDAFAKPLLTPFEAEVALGLIPG 392
           D+  VLMSEI P R+  F D +DAWIQ+ACPRLSIDWG AF  PLLTP+EA  A  ++P 
Sbjct: 348 DFVCVLMSEIFPSRLGQFSD-IDAWIQVACPRLSIDWGYAFPAPLLTPYEASAAFNVVP- 400

BLAST of CSPI07G05150 vs. ExPASy Swiss-Prot
Match: Q7SC98 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=dph-1 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 3.5e-101
Identity = 196/431 (45.48%), Postives = 269/431 (62.41%), Query Frame = 0

Query: 3   SELQNPSSELAVLSQSTEDKPPPR-SRPPPKRFVKNQIPDSILNDPALNAAISLLPSNYN 62
           S   N +S+ A  + S ED    + ++P     + NQ+PDSIL+D ALN AI+LLPSNY+
Sbjct: 49  SSSSNSTSQPAGANTSIEDSGAIQVAQPRRAPRMLNQVPDSILHDAALNEAIALLPSNYS 108

Query: 63  FEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCVD 122
           FE+HK + RI +  A RVALQ+PEGLL+++  +SDILT F       ++GDVT+GACC+D
Sbjct: 109 FEIHKTIHRIRTLDAKRVALQMPEGLLLFATTISDILTQFCPGIETLIMGDVTYGACCID 168

Query: 123 DLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTNL 182
           D +A A+  DLL+HY HSCL+P+D T I  LYVFVDI+ID   L+ T+  N       ++
Sbjct: 169 DYTARAMGCDLLVHYAHSCLIPVDVTKIKTLYVFVDISIDTSHLLATLERNF--APGKSI 228

Query: 183 VLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGET-V 242
            + GTIQF + I   +  L++ G  V+IPQ  PLS GE+LGCT+P +S     +  +  +
Sbjct: 229 AMVGTIQFNATIHGVRSTLQKAGYEVIIPQIAPLSKGEILGCTSPNLSQFLTSSGKQVDM 288

Query: 243 AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW 302
            +++ DGRFHLE+ MI NP I  +RYDPY  KL  E Y HE M+  R+ AI +AK A  W
Sbjct: 289 ILYLGDGRFHLESIMIHNPSIPAYRYDPYSRKLTHETYGHEEMQDVRRSAIRQAKTAKKW 348

Query: 303 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 362
           G++LG+LGRQGNP  L  +EEK+K+ G  +    +SEI P ++A+  D V+ W+Q+ACPR
Sbjct: 349 GLILGSLGRQGNPNTLGLIEEKLKANGTPFVNFCLSEIFPGKLAMMSD-VECWVQVACPR 408

Query: 363 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAEG 422
           LSIDWG AF +PLLTP+EA +AL     W                             +G
Sbjct: 409 LSIDWGYAFPRPLLTPYEALIALEEKEDW----------------------------GKG 448

Query: 423 EYPMDYYAQDG 432
            YPMDYY ++G
Sbjct: 469 AYPMDYYGREG 448

BLAST of CSPI07G05150 vs. ExPASy Swiss-Prot
Match: Q9BZG8 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Homo sapiens OX=9606 GN=DPH1 PE=1 SV=2)

HSP 1 Score: 367.5 bits (942), Expect = 2.3e-100
Identity = 189/362 (52.21%), Postives = 253/362 (69.89%), Query Frame = 0

Query: 24  PPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNYNFEVHKCVWRILSSSASRVALQL 83
           P R R P  R V NQIP  IL +P L AAI +LPSNYNFE+ K +WRI  + A +VALQ+
Sbjct: 23  PGRGRAPRGR-VANQIPPEILKNPQLQAAIRVLPSNYNFEIPKTIWRIQQAQAKKVALQM 82

Query: 84  PEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCVDDLSASALHADLLIHYGHSCLVP 143
           PEGLL+++  + DIL  F +A    V+GDVT+GACCVDD +A AL AD L+HYGHSCL+P
Sbjct: 83  PEGLLLFACTIVDILERFTEA-EVMVMGDVTYGACCVDDFTARALGADFLVHYGHSCLIP 142

Query: 144 IDSTT--IPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTNLVLAGTIQFASAIRASKMELE 203
           +D++      LYVFVDI ID   L++++RL      +T L L  TIQF S ++A+  EL+
Sbjct: 143 MDTSAQDFRVLYVFVDIRIDTTHLLDSLRLTFP--PATALALVSTIQFVSTLQAAAQELK 202

Query: 204 RHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETVAIFVADGRFHLEAFMIANPGI 263
               RV +PQ +PLS GE+LGCT+P++S            +++ DGRFHLE+ MIANP +
Sbjct: 203 AE-YRVSVPQCKPLSPGEILGCTSPRLSKEV------EAVVYLGDGRFHLESVMIANPNV 262

Query: 264 RVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNWGIVLGTLGRQGNPKILERLEE 323
             +RYDPY   L  E YDH+ M+A+R+ AI  A+ A +WG++LGTLGRQG+PKILE LE 
Sbjct: 263 PAYRYDPYSKVLSREHYDHQRMQAARQEAIATARSAKSWGLILGTLGRQGSPKILEHLES 322

Query: 324 KMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPRLSIDWGDAFAKPLLTPFEAEV 383
           ++++ G  +  +L+SEI P +++L  + VD W+Q+ACPRLSIDWG AF KPLLTP+EA V
Sbjct: 323 RLRALGLSFVRLLLSEIFPSKLSLLPE-VDVWVQVACPRLSIDWGTAFPKPLLTPYEAAV 372

BLAST of CSPI07G05150 vs. ExPASy TrEMBL
Match: A0A0A0K1X6 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Cucumis sativus OX=3659 GN=Csa_7G056490 PE=3 SV=1)

HSP 1 Score: 923.3 bits (2385), Expect = 4.0e-265
Identity = 460/463 (99.35%), Postives = 461/463 (99.57%), Query Frame = 0

Query: 1   MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60
           MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY
Sbjct: 1   MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60

Query: 61  NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV 120
           NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV
Sbjct: 61  NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV 120

Query: 121 DDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN 180
           DDLSASALHADLL+HYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN
Sbjct: 121 DDLSASALHADLLVHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN 180

Query: 181 LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV 240
           LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV
Sbjct: 181 LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV 240

Query: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW 300
           AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW
Sbjct: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW 300

Query: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360
           GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR
Sbjct: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360

Query: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAEG 420
           LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDE NKAEAEG
Sbjct: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDEINKAEAEG 420

Query: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCISDKAISLH 464
           EYPMDYYAQDGGEWNSSYVKKKVSRPVRRD VSCISDKAISLH
Sbjct: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPVRRD-VSCISDKAISLH 462

BLAST of CSPI07G05150 vs. ExPASy TrEMBL
Match: A0A5A7SKC8 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002900 PE=3 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 1.0e-260
Identity = 450/464 (96.98%), Postives = 460/464 (99.14%), Query Frame = 0

Query: 1   MKSELQNPSSELAVLSQST-EDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSN 60
           M+SE+Q PSSELAVLSQST ED+PPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSN
Sbjct: 1   MESEVQKPSSELAVLSQSTEEDRPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSN 60

Query: 61  YNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACC 120
           YNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACC
Sbjct: 61  YNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACC 120

Query: 121 VDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDST 180
           VDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDST
Sbjct: 121 VDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDST 180

Query: 181 NLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGET 240
           NLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGET
Sbjct: 181 NLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGET 240

Query: 241 VAIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATN 300
           VAIFVADGRFHLEAFMIANPGIRVFRYDPY+GKLLLEEYDHEGMKASRKGAILKAKEATN
Sbjct: 241 VAIFVADGRFHLEAFMIANPGIRVFRYDPYVGKLLLEEYDHEGMKASRKGAILKAKEATN 300

Query: 301 WGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACP 360
           WGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEI+PYRVALFEDSVDAWIQIACP
Sbjct: 301 WGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEINPYRVALFEDSVDAWIQIACP 360

Query: 361 RLSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAE 420
           RLSIDWGDAFAKPLLTPFEAEVALGLIPGWWER+++AKLSCNDCRECGCN D+T+KAEAE
Sbjct: 361 RLSIDWGDAFAKPLLTPFEAEVALGLIPGWWERSTAAKLSCNDCRECGCNSDQTDKAEAE 420

Query: 421 GEYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCISDKAISLH 464
           GEYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCI DKAISLH
Sbjct: 421 GEYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCIRDKAISLH 464

BLAST of CSPI07G05150 vs. ExPASy TrEMBL
Match: A0A1S3C024 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Cucumis melo OX=3656 GN=LOC103495461 PE=3 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 1.0e-260
Identity = 450/464 (96.98%), Postives = 460/464 (99.14%), Query Frame = 0

Query: 1   MKSELQNPSSELAVLSQST-EDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSN 60
           M+SE+Q PSSELAVLSQST ED+PPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSN
Sbjct: 1   MESEVQKPSSELAVLSQSTEEDRPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSN 60

Query: 61  YNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACC 120
           YNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACC
Sbjct: 61  YNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACC 120

Query: 121 VDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDST 180
           VDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDST
Sbjct: 121 VDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDST 180

Query: 181 NLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGET 240
           NLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGET
Sbjct: 181 NLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGET 240

Query: 241 VAIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATN 300
           VAIFVADGRFHLEAFMIANPGIRVFRYDPY+GKLLLEEYDHEGMKASRKGAILKAKEATN
Sbjct: 241 VAIFVADGRFHLEAFMIANPGIRVFRYDPYVGKLLLEEYDHEGMKASRKGAILKAKEATN 300

Query: 301 WGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACP 360
           WGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEI+PYRVALFEDSVDAWIQIACP
Sbjct: 301 WGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEINPYRVALFEDSVDAWIQIACP 360

Query: 361 RLSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAE 420
           RLSIDWGDAFAKPLLTPFEAEVALGLIPGWWER+++AKLSCNDCRECGCN D+T+KAEAE
Sbjct: 361 RLSIDWGDAFAKPLLTPFEAEVALGLIPGWWERSTAAKLSCNDCRECGCNSDQTDKAEAE 420

Query: 421 GEYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCISDKAISLH 464
           GEYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCI DKAISLH
Sbjct: 421 GEYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCIRDKAISLH 464

BLAST of CSPI07G05150 vs. ExPASy TrEMBL
Match: A0A6J1DM06 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Momordica charantia OX=3673 GN=LOC111021686 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 8.9e-241
Identity = 415/462 (89.83%), Postives = 439/462 (95.02%), Query Frame = 0

Query: 1   MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60
           M+SE+Q P + LAV+SQ TE K P RSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY
Sbjct: 1   MESEVQKPPA-LAVVSQGTEIKLPSRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60

Query: 61  NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV 120
           NFEVHKCVWRILS+ ASRVALQLPEGLLMYSLILSDILTTFG ASHCFVLGDVT+GACCV
Sbjct: 61  NFEVHKCVWRILSTGASRVALQLPEGLLMYSLILSDILTTFGNASHCFVLGDVTYGACCV 120

Query: 121 DDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN 180
           DDLSA+AL ADLLIHYGHSCL+PIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVD  DSTN
Sbjct: 121 DDLSAAALRADLLIHYGHSCLIPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDGFDSTN 180

Query: 181 LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV 240
           LVLAGTIQFASAIR +K+ELERHG RVLIPQSRPLSAGEVLGCTAPK+ ++AFGN+GETV
Sbjct: 181 LVLAGTIQFASAIRVAKVELERHGFRVLIPQSRPLSAGEVLGCTAPKIFSSAFGNSGETV 240

Query: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW 300
           AIFVADGRFHLEAFMIANPG+RVFRYDPY+GKLLLEEYDHEGMKASRKGA+LKA+EA NW
Sbjct: 241 AIFVADGRFHLEAFMIANPGMRVFRYDPYVGKLLLEEYDHEGMKASRKGAVLKAREARNW 300

Query: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360
           GIVLGTLGRQGNPKILERLEEKMKSKGF+YTVVLMSEISPYRVALFEDSVDAWIQIACPR
Sbjct: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFNYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360

Query: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAEG 420
           LSIDWGDAFAKPLLTPFEAE+ALGLI GWWERTSS KLSCNDC  CGCNGD+ ++ EAEG
Sbjct: 361 LSIDWGDAFAKPLLTPFEAEIALGLIHGWWERTSSVKLSCNDCEGCGCNGDQRDEVEAEG 420

Query: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCISDKAISL 463
           EYPMDYYAQ+GGEWNSSYVKKKVSRPVRRDVVSC SDKAISL
Sbjct: 421 EYPMDYYAQEGGEWNSSYVKKKVSRPVRRDVVSCTSDKAISL 461

BLAST of CSPI07G05150 vs. ExPASy TrEMBL
Match: A0A6J1JI80 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Cucurbita maxima OX=3661 GN=LOC111486020 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.1e-233
Identity = 409/451 (90.69%), Postives = 428/451 (94.90%), Query Frame = 0

Query: 1   MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60
           M+SE+Q P  ELA+LSQSTE  PP RSRPPPKRFVKNQIPDSILN+ ALNAAISLLPSNY
Sbjct: 1   MESEVQKP-PELALLSQSTEANPPSRSRPPPKRFVKNQIPDSILNNSALNAAISLLPSNY 60

Query: 61  NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV 120
           NFEVHKCVWRILSS ASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVT+GACCV
Sbjct: 61  NFEVHKCVWRILSSGASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTYGACCV 120

Query: 121 DDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN 180
           DDLSASAL+ADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLN+D L STN
Sbjct: 121 DDLSASALNADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNIDGLGSTN 180

Query: 181 LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV 240
           LVLAGTIQFASAIRA K+ELERHG RVLIPQSRPLSAGEVLGCTAPK+S++AFGN+GETV
Sbjct: 181 LVLAGTIQFASAIRAGKLELERHGFRVLIPQSRPLSAGEVLGCTAPKISSSAFGNDGETV 240

Query: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW 300
           AIFVADGRFHLEAFMIANPGIRVFRYDPY+GKLLLEEYDH GMKASRKGA+LKA+EA NW
Sbjct: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYVGKLLLEEYDHVGMKASRKGAVLKAREARNW 300

Query: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360
           GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR
Sbjct: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360

Query: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAEG 420
           LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERT++AKLSCNDC +CGCN D     + EG
Sbjct: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTNAAKLSCNDCEKCGCNSD-----QVEG 420

Query: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPV-RRD 451
           EYPMDYYAQDGGEWNSSYVKKKVSRPV RRD
Sbjct: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPVGRRD 445

BLAST of CSPI07G05150 vs. NCBI nr
Match: XP_011658762.1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Cucumis sativus] >KGN43670.1 hypothetical protein Csa_017201 [Cucumis sativus])

HSP 1 Score: 923.3 bits (2385), Expect = 8.2e-265
Identity = 460/463 (99.35%), Postives = 461/463 (99.57%), Query Frame = 0

Query: 1   MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60
           MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY
Sbjct: 1   MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60

Query: 61  NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV 120
           NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV
Sbjct: 61  NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV 120

Query: 121 DDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN 180
           DDLSASALHADLL+HYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN
Sbjct: 121 DDLSASALHADLLVHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN 180

Query: 181 LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV 240
           LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV
Sbjct: 181 LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV 240

Query: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW 300
           AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW
Sbjct: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW 300

Query: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360
           GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR
Sbjct: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360

Query: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAEG 420
           LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDE NKAEAEG
Sbjct: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDEINKAEAEG 420

Query: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCISDKAISLH 464
           EYPMDYYAQDGGEWNSSYVKKKVSRPVRRD VSCISDKAISLH
Sbjct: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPVRRD-VSCISDKAISLH 462

BLAST of CSPI07G05150 vs. NCBI nr
Match: XP_008455253.2 (PREDICTED: diphthamide biosynthesis protein 1 [Cucumis melo] >KAA0031522.1 diphthamide biosynthesis protein 1 [Cucumis melo var. makuwa] >TYK06975.1 diphthamide biosynthesis protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 908.7 bits (2347), Expect = 2.1e-260
Identity = 450/464 (96.98%), Postives = 460/464 (99.14%), Query Frame = 0

Query: 1   MKSELQNPSSELAVLSQST-EDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSN 60
           M+SE+Q PSSELAVLSQST ED+PPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSN
Sbjct: 1   MESEVQKPSSELAVLSQSTEEDRPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSN 60

Query: 61  YNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACC 120
           YNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACC
Sbjct: 61  YNFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACC 120

Query: 121 VDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDST 180
           VDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDST
Sbjct: 121 VDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDST 180

Query: 181 NLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGET 240
           NLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGET
Sbjct: 181 NLVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGET 240

Query: 241 VAIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATN 300
           VAIFVADGRFHLEAFMIANPGIRVFRYDPY+GKLLLEEYDHEGMKASRKGAILKAKEATN
Sbjct: 241 VAIFVADGRFHLEAFMIANPGIRVFRYDPYVGKLLLEEYDHEGMKASRKGAILKAKEATN 300

Query: 301 WGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACP 360
           WGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEI+PYRVALFEDSVDAWIQIACP
Sbjct: 301 WGIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEINPYRVALFEDSVDAWIQIACP 360

Query: 361 RLSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAE 420
           RLSIDWGDAFAKPLLTPFEAEVALGLIPGWWER+++AKLSCNDCRECGCN D+T+KAEAE
Sbjct: 361 RLSIDWGDAFAKPLLTPFEAEVALGLIPGWWERSTAAKLSCNDCRECGCNSDQTDKAEAE 420

Query: 421 GEYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCISDKAISLH 464
           GEYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCI DKAISLH
Sbjct: 421 GEYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCIRDKAISLH 464

BLAST of CSPI07G05150 vs. NCBI nr
Match: XP_038888490.1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Benincasa hispida])

HSP 1 Score: 874.8 bits (2259), Expect = 3.3e-250
Identity = 431/463 (93.09%), Postives = 449/463 (96.98%), Query Frame = 0

Query: 1   MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60
           M+SE+Q P SEL +LSQSTEDKPPPRSRPPPKRFVKNQIPDSIL DPALNAAISLLPSNY
Sbjct: 1   MESEVQKP-SELDILSQSTEDKPPPRSRPPPKRFVKNQIPDSILKDPALNAAISLLPSNY 60

Query: 61  NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV 120
           NFEVHKCVWRILSS ASR+ALQLPEGLLMYSLILSDILTTFG AS CFVLGDVT+GACCV
Sbjct: 61  NFEVHKCVWRILSSGASRIALQLPEGLLMYSLILSDILTTFGNASDCFVLGDVTYGACCV 120

Query: 121 DDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN 180
           DDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNV  LDSTN
Sbjct: 121 DDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVHGLDSTN 180

Query: 181 LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV 240
           LVLAGTIQFASAIRASKMELERHGIRVLIPQS+PLSAGEVLGCTAPK+ST++FGNNGETV
Sbjct: 181 LVLAGTIQFASAIRASKMELERHGIRVLIPQSKPLSAGEVLGCTAPKISTSSFGNNGETV 240

Query: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW 300
           AIFVADGRFHLEAFMIANPGIRVFRYDPY+GKLLLEEYDHEGMKASRKGA+LKAKEA NW
Sbjct: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYVGKLLLEEYDHEGMKASRKGAVLKAKEARNW 300

Query: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360
           GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR
Sbjct: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360

Query: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAEG 420
           LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTS+AKLSCNDC+ C CNGD+++K EAEG
Sbjct: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSAAKLSCNDCQGCECNGDQSDKVEAEG 420

Query: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCISDKAISLH 464
           EYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCIS+K +SLH
Sbjct: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCISEKPVSLH 462

BLAST of CSPI07G05150 vs. NCBI nr
Match: XP_022154409.1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Momordica charantia] >XP_022154410.1 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Momordica charantia])

HSP 1 Score: 842.4 bits (2175), Expect = 1.8e-240
Identity = 415/462 (89.83%), Postives = 439/462 (95.02%), Query Frame = 0

Query: 1   MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60
           M+SE+Q P + LAV+SQ TE K P RSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY
Sbjct: 1   MESEVQKPPA-LAVVSQGTEIKLPSRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60

Query: 61  NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV 120
           NFEVHKCVWRILS+ ASRVALQLPEGLLMYSLILSDILTTFG ASHCFVLGDVT+GACCV
Sbjct: 61  NFEVHKCVWRILSTGASRVALQLPEGLLMYSLILSDILTTFGNASHCFVLGDVTYGACCV 120

Query: 121 DDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN 180
           DDLSA+AL ADLLIHYGHSCL+PIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVD  DSTN
Sbjct: 121 DDLSAAALRADLLIHYGHSCLIPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDGFDSTN 180

Query: 181 LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV 240
           LVLAGTIQFASAIR +K+ELERHG RVLIPQSRPLSAGEVLGCTAPK+ ++AFGN+GETV
Sbjct: 181 LVLAGTIQFASAIRVAKVELERHGFRVLIPQSRPLSAGEVLGCTAPKIFSSAFGNSGETV 240

Query: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW 300
           AIFVADGRFHLEAFMIANPG+RVFRYDPY+GKLLLEEYDHEGMKASRKGA+LKA+EA NW
Sbjct: 241 AIFVADGRFHLEAFMIANPGMRVFRYDPYVGKLLLEEYDHEGMKASRKGAVLKAREARNW 300

Query: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360
           GIVLGTLGRQGNPKILERLEEKMKSKGF+YTVVLMSEISPYRVALFEDSVDAWIQIACPR
Sbjct: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFNYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360

Query: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAEG 420
           LSIDWGDAFAKPLLTPFEAE+ALGLI GWWERTSS KLSCNDC  CGCNGD+ ++ EAEG
Sbjct: 361 LSIDWGDAFAKPLLTPFEAEIALGLIHGWWERTSSVKLSCNDCEGCGCNGDQRDEVEAEG 420

Query: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPVRRDVVSCISDKAISL 463
           EYPMDYYAQ+GGEWNSSYVKKKVSRPVRRDVVSC SDKAISL
Sbjct: 421 EYPMDYYAQEGGEWNSSYVKKKVSRPVRRDVVSCTSDKAISL 461

BLAST of CSPI07G05150 vs. NCBI nr
Match: XP_022988779.1 (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Cucurbita maxima] >XP_022988781.1 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Cucurbita maxima] >XP_022988782.1 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Cucurbita maxima])

HSP 1 Score: 818.9 bits (2114), Expect = 2.2e-233
Identity = 409/451 (90.69%), Postives = 428/451 (94.90%), Query Frame = 0

Query: 1   MKSELQNPSSELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNY 60
           M+SE+Q P  ELA+LSQSTE  PP RSRPPPKRFVKNQIPDSILN+ ALNAAISLLPSNY
Sbjct: 1   MESEVQKP-PELALLSQSTEANPPSRSRPPPKRFVKNQIPDSILNNSALNAAISLLPSNY 60

Query: 61  NFEVHKCVWRILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCV 120
           NFEVHKCVWRILSS ASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVT+GACCV
Sbjct: 61  NFEVHKCVWRILSSGASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTYGACCV 120

Query: 121 DDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTN 180
           DDLSASAL+ADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLN+D L STN
Sbjct: 121 DDLSASALNADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNIDGLGSTN 180

Query: 181 LVLAGTIQFASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETV 240
           LVLAGTIQFASAIRA K+ELERHG RVLIPQSRPLSAGEVLGCTAPK+S++AFGN+GETV
Sbjct: 181 LVLAGTIQFASAIRAGKLELERHGFRVLIPQSRPLSAGEVLGCTAPKISSSAFGNDGETV 240

Query: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNW 300
           AIFVADGRFHLEAFMIANPGIRVFRYDPY+GKLLLEEYDH GMKASRKGA+LKA+EA NW
Sbjct: 241 AIFVADGRFHLEAFMIANPGIRVFRYDPYVGKLLLEEYDHVGMKASRKGAVLKAREARNW 300

Query: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360
           GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR
Sbjct: 301 GIVLGTLGRQGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPR 360

Query: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCRECGCNGDETNKAEAEG 420
           LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERT++AKLSCNDC +CGCN D     + EG
Sbjct: 361 LSIDWGDAFAKPLLTPFEAEVALGLIPGWWERTNAAKLSCNDCEKCGCNSD-----QVEG 420

Query: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPV-RRD 451
           EYPMDYYAQDGGEWNSSYVKKKVSRPV RRD
Sbjct: 421 EYPMDYYAQDGGEWNSSYVKKKVSRPVGRRD 445

BLAST of CSPI07G05150 vs. TAIR 10
Match: AT5G62030.1 (diphthamide synthesis DPH2 family protein )

HSP 1 Score: 608.2 bits (1567), Expect = 5.4e-174
Identity = 305/438 (69.63%), Postives = 354/438 (80.82%), Query Frame = 0

Query: 26  RSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNYNFEVHKCVWRILSSSASRVALQLPE 85
           + + PPKRF+KNQIPDSIL D +LNAAIS+LPS Y+FEVHKCVWRI +S+A R+ALQLPE
Sbjct: 13  KPKQPPKRFIKNQIPDSILGDASLNAAISILPSIYHFEVHKCVWRIKTSNAKRIALQLPE 72

Query: 86  GLLMYSLILSDILTTFGKASHCFVLGDVTFGACCVDDLSASALHADLLIHYGHSCLVPID 145
           GLLMY+L LSDI T+F  ASHCFVLGDVT+GACCVDD SA AL ADLLIHYGHSCLVPID
Sbjct: 73  GLLMYALTLSDIFTSFAGASHCFVLGDVTYGACCVDDFSACALGADLLIHYGHSCLVPID 132

Query: 146 STTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTNLVLAGTIQFASAIRASKMELERHGI 205
           ST IPCLYVFV+I IDV  L+NT+ LN+   D  N++LAGTIQF SAIRA K ELE+ G 
Sbjct: 133 STKIPCLYVFVEIQIDVKCLLNTIHLNL-ASDVKNIILAGTIQFTSAIRAVKPELEKQGF 192

Query: 206 RVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETVAIFVADGRFHLEAFMIANPGIRVFR 265
            VLIPQS+PLSAGEVLGCTAPKV T    +  + V +FVADGRFHLEAFMIANP I+ FR
Sbjct: 193 NVLIPQSKPLSAGEVLGCTAPKVKTVE--DCKDQVLVFVADGRFHLEAFMIANPKIKAFR 252

Query: 266 YDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNWGIVLGTLGRQGNPKILERLEEKMKS 325
           YDPY+GKL LEEYDH+GM+ +R  AI +A+EA  WGIVLGTLGRQGNPKILERLE+KM  
Sbjct: 253 YDPYLGKLFLEEYDHKGMRETRMRAIARAREAKTWGIVLGTLGRQGNPKILERLEKKMME 312

Query: 326 KGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPRLSIDWGDAFAKPLLTPFEAEVALGL 385
           KG D TVVLMSE+SP RVALFEDSVDAW+QIACPRLSIDWG+AF KPLLT FEAE+ALG 
Sbjct: 313 KGIDSTVVLMSELSPTRVALFEDSVDAWVQIACPRLSIDWGEAFLKPLLTTFEAEIALGF 372

Query: 386 IPGWWERTSSAKLSCND--CRE-----CGCNGD------ETNKAEAEGEYPMDYYAQDGG 445
           I GWWE  SS++++ +   C+E     C C  +      + N    +G+YPMDYYAQ+GG
Sbjct: 373 IRGWWENDSSSRVNSSSGCCKEDKETSCACKNENVKDDKKDNDGALDGDYPMDYYAQEGG 432

Query: 446 EWNSSYVKKKVSRPVRRD 451
           EWNSSY+KK  SRP+RR+
Sbjct: 433 EWNSSYLKKS-SRPIRRN 446

BLAST of CSPI07G05150 vs. TAIR 10
Match: AT3G59630.1 (diphthamide synthesis DPH2 family protein )

HSP 1 Score: 103.2 bits (256), Expect = 5.7e-22
Identity = 85/362 (23.48%), Postives = 160/362 (44.20%), Query Frame = 0

Query: 62  FEVHKCVWRILSSSASRVALQLPEGLL-----MYSLILSDILTTFGKASHCFVLGDVTFG 121
           +E+++    I+S S +R+ALQ P+ LL     + S + S       +    FV+ D T+G
Sbjct: 9   YEINRTAEFIISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFFVMADTTYG 68

Query: 122 ACCVDDLSASALHADLLIHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCL 181
           +CC+D++ A  + ++ ++HYG +CL P  ++ +P  +VF   +I+V   V  +       
Sbjct: 69  SCCIDEVGALHIDSECVVHYGQTCLSP--TSVLPAFFVFGKASINVSSCVKHLIDYASKS 128

Query: 182 DSTNLVLAGTIQFASAIRASKMEL------------------------ERHGIRVLIPQS 241
           D   ++L G +++A  I   + EL                         R  +    P S
Sbjct: 129 DKPIMILYG-LEYAHVIPQIREELGLSKTDSQLSVANVLCSFISPSKDPRESMEHPRPYS 188

Query: 242 RPLSAGEVLGCTAPKVSTAAF----GNNGETVAIF-VADGRFHLEAFMIANPGIRVFRYD 301
              S+  +    + ++    +    G+  E   +F +          ++   G  + RYD
Sbjct: 189 ESDSSDSLSSSRSYRLGGLTWDLPEGSKIEDYLLFWIGSDSSAFANVVLTFNGCDIVRYD 248

Query: 302 PYIGKLLLEEYDHEGMKASRKGAILKAKEATNWGIVLGTLGRQGNPKILERLEEKMKSKG 361
                L+ E Y    +   R   + KAK+A   GI++GTLG  G   ++  ++  + + G
Sbjct: 249 AEEDSLVTEFYQQRRILKRRYYLVEKAKDANIIGILVGTLGVAGYLHMIHHMQALISAAG 308

Query: 362 FDYTVVLMSEISPYRVALFEDSVDAWIQIACPRLSIDWGDAFAKPLLTPFEAEVALGLIP 390
               ++ M   +P ++A F +  D +I I+C + ++     F  P++TPFEA +A     
Sbjct: 309 KKSYILAMGRPNPAKLANFPE-CDVFIYISCAQTALLDSKEFMSPVITPFEANLAFSRGS 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54PW54.7e-10648.132-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Dictyostelium discoid... [more]
Q4IQ721.9e-10248.102-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Gibberella zeae (stra... [more]
O597139.2e-10251.792-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Schizosaccharomyces p... [more]
Q7SC983.5e-10145.482-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Neurospora crassa (st... [more]
Q9BZG82.3e-10052.212-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Homo sapiens OX=9606 ... [more]
Match NameE-valueIdentityDescription
A0A0A0K1X64.0e-26599.352-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Cucumis sativus OX=36... [more]
A0A5A7SKC81.0e-26096.982-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Cucumis melo var. mak... [more]
A0A1S3C0241.0e-26096.982-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Cucumis melo OX=3656 ... [more]
A0A6J1DM068.9e-24189.832-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Momordica charantia O... [more]
A0A6J1JI801.1e-23390.692-(3-amino-3-carboxypropyl)histidine synthase subunit 1 OS=Cucurbita maxima OX=3... [more]
Match NameE-valueIdentityDescription
XP_011658762.18.2e-26599.352-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Cucumis sativus] >KGN43... [more]
XP_008455253.22.1e-26096.98PREDICTED: diphthamide biosynthesis protein 1 [Cucumis melo] >KAA0031522.1 dipht... [more]
XP_038888490.13.3e-25093.092-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Benincasa hispida][more]
XP_022154409.11.8e-24089.832-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Momordica charantia] >X... [more]
XP_022988779.12.2e-23390.692-(3-amino-3-carboxypropyl)histidine synthase subunit 1 [Cucurbita maxima] >XP_0... [more]
Match NameE-valueIdentityDescription
AT5G62030.15.4e-17469.63diphthamide synthesis DPH2 family protein [more]
AT3G59630.15.7e-2223.48diphthamide synthesis DPH2 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042264Diphthamide synthesis DPH1/DPH2, domain 2GENE3D3.40.50.11850Diphthamide synthesis DPH1/DPH2 domain 2coord: 157..279
e-value: 2.1E-102
score: 344.4
IPR035435Diphthamide synthesis DHP1/DPH2, eukaryotes and archaeaPIRSFPIRSF004967DPH1coord: 43..444
e-value: 1.4E-112
score: 374.0
IPR016435Diphthamide synthesis DPH1/DPH2TIGRFAMTIGR00322TIGR00322coord: 60..383
e-value: 4.5E-109
score: 362.7
IPR016435Diphthamide synthesis DPH1/DPH2PFAMPF01866Diphthamide_syncoord: 83..387
e-value: 6.1E-105
score: 350.9
IPR016435Diphthamide synthesis DPH1/DPH2SFLDSFLDS00032Radical_SAM_3-amino-3-carboxycoord: 24..442
e-value: 0.0
score: 346.6
IPR016435Diphthamide synthesis DPH1/DPH2PANTHERPTHR10762DIPHTHAMIDE BIOSYNTHESIS PROTEINcoord: 23..451
IPR042263Diphthamide synthesis DPH1/DPH2, domain 1GENE3D3.40.50.11840Diphthamide synthesis DPH1/DPH2 domain 1coord: 73..156
e-value: 2.1E-102
score: 344.4
IPR042265Diphthamide synthesis DPH1/DPH2, domain 3GENE3D3.40.50.11860Diphthamide synthesis DPH1/DPH2 domain 3coord: 280..380
e-value: 2.1E-102
score: 344.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR10762:SF12-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1coord: 23..451

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G05150.1CSPI07G05150.1mRNA
CSPI07G05150.3CSPI07G05150.3mRNA
CSPI07G05150.2CSPI07G05150.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine
molecular_function GO:0090560 2-(3-amino-3-carboxypropyl)histidine synthase activity