CSPI07G05010 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G05010
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDiacylglycerol O-acyltransferase
LocationChr7: 3709278 .. 3711171 (+)
RNA-Seq ExpressionCSPI07G05010
SyntenyCSPI07G05010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAGCTCATTTGTTCAAGTATCCAACATCCCCGACCGTCGCTGGAACCATGGTTCTAAAGGTCCACCACTCCCTCGGCGATGGCTATTCTCTCATGGGTGTCGTTCTTTCTTGTCTTCACACAGCTTCAGACCTAACGTTCCTCTCACTTTTCCTTCCCGGCGTCAACGACGATTCAAGGACGGTGTTGTGAAGTCTTTGACTGGAATTGTTTCCGCTGTTTGTAGAACGGCGTCGGATTTGAGGTGGAGTGTTTTGAAAGGAAAAGATGAGAAAACTTCTATAAGGTCAGGACGTGAAGGGCTACAATTCATGCCGATGAATACTTTTACGGTGTCCTTTTCGCTCAATTACATAAAGAAAATCAAGACGAAGCTCGACGTGGTGAGTTTTGTAATGAAAAACGCATACCTCTATATTGAAGCTGCGAAAAAAAATATTCATATCTGTGTGTCCATGCCTGCATACACCTACCTCAACTGAATGTCAAGAAAATTTATAAAACGATATCATCATGAATTAATTGAATTCATCCTTCTCAGTTATGTATTAAACTCGCATAACCCTTTTAACCACTAGAGTTATAACAATTTATTTTAGTCTTTCAGTTTTCGAAATGCCCAATTTAGCCATTAGTTCAGCTTTTTAAGAAAAAAAAAAGTACCATTGTTTGGTCGTTACCATATGATCTTGTCACGTCAAATTTGCCTCAATGGCTTGAATACATATTCATATTAGGTACATGACTATGCATATATGTTATTTATTTTATACTAACGAGTGTTATTTTTTTTTTCACTGAAAAATGCAGACAGTAAATGATGTGATAACAGGGATAACCTTCATGGGACTAAGGTTGTACATGGAAGAAATGGAGAAAAATTCCGGTGAAGTCGCAGCGACTGCATTGACAATAGTGAACACGAGAGTGATTGGAACCTACCGGCCGGCGACAGAGATGGCAAAACCAGAGAGCAAAGGACTATGGGGAAACCAATTATCTTACTTGGAAACTATGATCCCAAAAATGACCAACACTAACCCACTTGAGTTTGTCGTGGCTGCTCATCGCTCCATCAACAACAAACGAAACTCCTTCACTATTCATGTTGTGGCTCATCTTTTGAATCTCTTGAGAAAACTTCGTGGTCATCAGGTAAAATTATTACTATATAATATTACTCAAATTTTAGCTTTAACTATTGTTTTCGATTTGACCAATCCCATACGTTTTGGTTTACTATGGTTCAAAAGCTCGATCTTCATCATTAGTATTGTTTTAAAAAAAAAAGACTTGGGTGCATCCTCTAGTTCTATTATATAACTGCTAAAAAAATGTATACGTACGTAGAGATTCAAGTTTGAAACTAAGTAAATTTCAATAATTAAGACTTCATGTATAACATTTTTAAAAATCGTAACAGATTTTGCTATATTTCACACTTTGTGTATATAATTTGGTAGGAAGTAGCAAAATTCCTCCACAATTTATTGAAGAACACAACCACAGTAATCTCAAATGTGATTGATCCACTTCAGCAAATGGCTTTAGATAATCATCCCATCAGCGGCTTATACTTCACCATCGTGGGCACACCTCAGGTTTTTATTTCATTATTTATTTATATATAATATAGCTTTAAATTCAATAATTTTTTCACGAAAGAGAGAGAGAGAGAAACAAAAAATCATTAAAAAAAGTTAATATGTGTGTGTGTATTTTTCTAATTTTTTGCAGAGTGTTACGGTAACGATGTTGAGTTATATGGGGAAACTGAGAGTTGCATTCAGAACAGAAAAGGATTTCATTGATGCCCACGAGTTAAATTCTTGTATGGAAGATGCATTTAAGAAGATATTTGAAGCAGCAAATGATATTCCTTCACCGTAA

mRNA sequence

ATGGGAAGCTCATTTGTTCAAGTATCCAACATCCCCGACCGTCGCTGGAACCATGGTTCTAAAGGTCCACCACTCCCTCGGCGATGGCTATTCTCTCATGGGTGTCGTTCTTTCTTGTCTTCACACAGCTTCAGACCTAACGTTCCTCTCACTTTTCCTTCCCGGCGTCAACGACGATTCAAGGACGGTGTTGTGAAGTCTTTGACTGGAATTGTTTCCGCTGTTTGTAGAACGGCGTCGGATTTGAGGTGGAGTGTTTTGAAAGGAAAAGATGAGAAAACTTCTATAAGGTCAGGACGTGAAGGGCTACAATTCATGCCGATGAATACTTTTACGGTGTCCTTTTCGCTCAATTACATAAAGAAAATCAAGACGAAGCTCGACGTGACAGTAAATGATGTGATAACAGGGATAACCTTCATGGGACTAAGGTTGTACATGGAAGAAATGGAGAAAAATTCCGGTGAAGTCGCAGCGACTGCATTGACAATAGTGAACACGAGAGTGATTGGAACCTACCGGCCGGCGACAGAGATGGCAAAACCAGAGAGCAAAGGACTATGGGGAAACCAATTATCTTACTTGGAAACTATGATCCCAAAAATGACCAACACTAACCCACTTGAGTTTGTCGTGGCTGCTCATCGCTCCATCAACAACAAACGAAACTCCTTCACTATTCATGTTGTGGCTCATCTTTTGAATCTCTTGAGAAAACTTCGTGGTCATCAGGAAGTAGCAAAATTCCTCCACAATTTATTGAAGAACACAACCACAGTAATCTCAAATGTGATTGATCCACTTCAGCAAATGGCTTTAGATAATCATCCCATCAGCGGCTTATACTTCACCATCGTGGGCACACCTCAGAGTGTTACGGTAACGATGTTGAGTTATATGGGGAAACTGAGAGTTGCATTCAGAACAGAAAAGGATTTCATTGATGCCCACGAGTTAAATTCTTGTATGGAAGATGCATTTAAGAAGATATTTGAAGCAGCAAATGATATTCCTTCACCGTAA

Coding sequence (CDS)

ATGGGAAGCTCATTTGTTCAAGTATCCAACATCCCCGACCGTCGCTGGAACCATGGTTCTAAAGGTCCACCACTCCCTCGGCGATGGCTATTCTCTCATGGGTGTCGTTCTTTCTTGTCTTCACACAGCTTCAGACCTAACGTTCCTCTCACTTTTCCTTCCCGGCGTCAACGACGATTCAAGGACGGTGTTGTGAAGTCTTTGACTGGAATTGTTTCCGCTGTTTGTAGAACGGCGTCGGATTTGAGGTGGAGTGTTTTGAAAGGAAAAGATGAGAAAACTTCTATAAGGTCAGGACGTGAAGGGCTACAATTCATGCCGATGAATACTTTTACGGTGTCCTTTTCGCTCAATTACATAAAGAAAATCAAGACGAAGCTCGACGTGACAGTAAATGATGTGATAACAGGGATAACCTTCATGGGACTAAGGTTGTACATGGAAGAAATGGAGAAAAATTCCGGTGAAGTCGCAGCGACTGCATTGACAATAGTGAACACGAGAGTGATTGGAACCTACCGGCCGGCGACAGAGATGGCAAAACCAGAGAGCAAAGGACTATGGGGAAACCAATTATCTTACTTGGAAACTATGATCCCAAAAATGACCAACACTAACCCACTTGAGTTTGTCGTGGCTGCTCATCGCTCCATCAACAACAAACGAAACTCCTTCACTATTCATGTTGTGGCTCATCTTTTGAATCTCTTGAGAAAACTTCGTGGTCATCAGGAAGTAGCAAAATTCCTCCACAATTTATTGAAGAACACAACCACAGTAATCTCAAATGTGATTGATCCACTTCAGCAAATGGCTTTAGATAATCATCCCATCAGCGGCTTATACTTCACCATCGTGGGCACACCTCAGAGTGTTACGGTAACGATGTTGAGTTATATGGGGAAACTGAGAGTTGCATTCAGAACAGAAAAGGATTTCATTGATGCCCACGAGTTAAATTCTTGTATGGAAGATGCATTTAAGAAGATATTTGAAGCAGCAAATGATATTCCTTCACCGTAA

Protein sequence

MGSSFVQVSNIPDRRWNHGSKGPPLPRRWLFSHGCRSFLSSHSFRPNVPLTFPSRRQRRFKDGVVKSLTGIVSAVCRTASDLRWSVLKGKDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAATALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKIFEAANDIPSP*
Homology
BLAST of CSPI07G05010 vs. ExPASy Swiss-Prot
Match: Q9FK89 (Wax ester synthase/diacylglycerol acyltransferase 9 OS=Arabidopsis thaliana OX=3702 GN=WSD9 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.2e-21
Identity = 68/231 (29.44%), Postives = 114/231 (49.35%), Query Frame = 0

Query: 116 SLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAATALT------------ 175
           SL+ IK IK  +++T+NDV+ G+T   L  Y+ +    + E A T+LT            
Sbjct: 239 SLDDIKLIKNAMEMTINDVLLGVTEAALTRYLHQSYDKTNEEAGTSLTPNRQDLLDRIRL 298

Query: 176 ----IVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTNTNPLEFVVAAHRSIN 235
               +VN R  G+   A  MAK  SK  WGN +S +         ++PL ++      I+
Sbjct: 299 RSLIVVNLRPTGSQSIADMMAK-GSKCRWGNYISVILFPFTIALQSDPLVYLSNVKSMID 358

Query: 236 NKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNHPIS 295
            K+NS   +++      + K  G      F   ++ NTT  ISN+  P ++++   HPI+
Sbjct: 359 RKKNSLITYIIYTFSEFVIKAFGINVAVAFQRKIMLNTTMCISNLPGPTEEVSFHGHPIA 418

Query: 296 GLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKI 331
               +I G PQ++T+  LSY  K+ ++   +   IDAH+L   +E++ K +
Sbjct: 419 YFAPSIYGLPQALTIHYLSYANKMIISVAVDPMIIDAHKLCDELEESLKNM 468

BLAST of CSPI07G05010 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 5.0e-20
Identity = 67/253 (26.48%), Postives = 120/253 (47.43%), Query Frame = 0

Query: 90  KDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGL-----R 149
           KD +T I+      +   +     + SL+ IK  K  +++T+NDV+ G+T  GL     R
Sbjct: 232 KDTETPIKGDFLSTKSKQLRLVHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLAR 291

Query: 150 LYMEEMEKNSGE------VAATALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETM 209
            Y EE  KN  +      +   +  +VN R     +   +M +  SK  WGN   Y+   
Sbjct: 292 RYGEEETKNRKQKKLPKRIRLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFP 351

Query: 210 IPKMTNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTT 269
                  +PLE +  A ++I+ K+NS+   +      ++ KL G +  A  ++ ++ NTT
Sbjct: 352 FSIALRDDPLEHLEIAQKTISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTT 411

Query: 270 TVISNVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFI-DAH 329
              SN++ P+++++   HPI+    +  G P ++T+   SYM K+ +    +   I D H
Sbjct: 412 MTFSNMVGPVEEVSFYGHPITYFASSAYGHPHALTIHCQSYMNKMTITLIVDPTVISDPH 471

Query: 330 ELNSCMEDAFKKI 331
            L    E++ + I
Sbjct: 472 RLCDDWEESLRSI 484

BLAST of CSPI07G05010 vs. ExPASy Swiss-Prot
Match: Q94CK0 (Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=WSD7 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.5e-19
Identity = 68/260 (26.15%), Postives = 131/260 (50.38%), Query Frame = 0

Query: 85  SVLKGKDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLR 144
           +VL  KD KT +++G + ++  P   +    SL+ IK IK  +++T+NDV+ GIT   L 
Sbjct: 210 TVLFLKDTKTPLKAGAD-VRSNPKRFYHRIISLDDIKLIKNAMNMTINDVLFGITQASLS 269

Query: 145 LYMEEM--EKNSGEVAATA-------------LTIVNTRVIGTYRPATEMAKPESKGLWG 204
            Y+      K   + A T+                VN R    ++P  +M   +SK  WG
Sbjct: 270 HYLNRQYGTKKEEDGALTSYRNNLPDGIRFRVACTVNLRSDIGFKPLADMMVKDSKCRWG 329

Query: 205 NQLSYLETMIPKMTNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKF 264
           N  S++         T+PL ++  +   +  K++S+   +V  ++ ++ K+ G +  A+ 
Sbjct: 330 NYFSFIFLPFTIGLQTDPLVYLKMSKSMMARKKHSYHAALVYFIIKIVLKVFGAKAAAEL 389

Query: 265 LHNLLKNTTTVISNVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRT 324
               ++NTTT +SNVI P+++++   HP+S +  +  G   ++ + ++SY  K+ ++   
Sbjct: 390 FDRPVRNTTTCVSNVIGPMEEISFRGHPVSYIAPSSYGHSHALLIHLMSYADKMIISLAY 449

Query: 325 EKDFI-DAHELNSCMEDAFK 329
           +   I D H++   ME++ K
Sbjct: 450 DPTVISDPHKICDDMEESLK 468

BLAST of CSPI07G05010 vs. ExPASy Swiss-Prot
Match: Q9FFE8 (Wax ester synthase/diacylglycerol acyltransferase 8 OS=Arabidopsis thaliana OX=3702 GN=WSD8 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.2e-18
Identity = 74/307 (24.10%), Postives = 140/307 (45.60%), Query Frame = 0

Query: 53  PSRRQRRFKDGVVKSLTGIVSAVCRTASDLR--WSVLKG-----------KDEKTSIRSG 112
           PS ++R      +K     ++A+    S +R  W+ L             KD KT ++ G
Sbjct: 165 PSVKRRSTVSHSLKKTGWFLTAIFTIGSTMRLIWNTLVDMFLLFATMLFLKDTKTPLKGG 224

Query: 113 REGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYME---EMEKNSGE 172
              ++  P   +  + SL+ IK IK  +++T+NDV+ GIT   L  Y+    E E N+ E
Sbjct: 225 -ANVRSNPKTFYHRNISLDDIKLIKNAMNMTINDVLLGITQAALSSYLNRRYEHENNNEE 284

Query: 173 --------------VAATALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKM 232
                         +   A   VN R    ++P  EM   +SK  WGN  S++   +   
Sbjct: 285 DGVLTSYTNNLPDRIRFRAGCTVNLRSDIGFKPLAEMMVKDSKCRWGNYFSFIILPLSIS 344

Query: 233 TNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVIS 292
             T+PL ++  +   +   ++S+   +   L+ +  K+ G +      +  L N TT +S
Sbjct: 345 LETDPLVYLNKSKAMMARTKHSYQAALTYFLIKISLKVLGAKATTSLFNQHLMNITTCVS 404

Query: 293 NVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFI-DAHELNS 329
           NV+ P+++++ + HP++ +  +  G   ++ +   SY G++ +    +   I D H++  
Sbjct: 405 NVMGPMEEISFNGHPVAYISPSSYGHSHALLIHYTSYAGEMTITITVDPTVIPDPHKICD 464

BLAST of CSPI07G05010 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.6e-18
Identity = 73/272 (26.84%), Postives = 130/272 (47.79%), Query Frame = 0

Query: 71  IVSAVCRTASDLRWSVLKGKDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVT 130
           +++ VC  A +   + L  KD +T I+   +  +   M     + SL+ IK IK  + +T
Sbjct: 226 VLNTVC-DALEFIATALFLKDTETPIKGDFKLSKGKRMCMVHRTVSLDDIKLIKNAMKMT 285

Query: 131 VNDVITGITFMGLRLYM-------EEMEKNSGE----VAATALTIVNTRVIGTYRPATEM 190
           VNDV+ G++  GL  Y+       EE ++NS      +   A  +VN R     +   +M
Sbjct: 286 VNDVVLGVSQAGLSQYLKRRYGEQEESKRNSSNIPKGIRLRAALLVNLRPTTGIQDLADM 345

Query: 191 AKPESKGLWGNQLSYLETMIPKMTNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRK 250
               SK  WGN + Y+          +PL+ +  A  +I+ K+NS    +   +  +L  
Sbjct: 346 MTKGSKCRWGNWIGYIIFPFSIALCDDPLKHLRRAKSTIDRKKNSLEAVLTFVVGKILLN 405

Query: 251 LRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSY 310
             G Q  A  L+  L NTT   SN++ P+++++   H ++ +  ++ G P ++T+   SY
Sbjct: 406 TLGVQRAANVLNRALSNTTMSFSNLVGPVEEISFYGHTVTYIAPSVYGHPHALTMHFQSY 465

Query: 311 MGKLRVAFRTEKDFI-DAHELNSCMEDAFKKI 331
           M KL ++   +   I D H+L    E++ + I
Sbjct: 466 MNKLTISLTVDPTVISDPHKLCDDWEESLRSI 496

BLAST of CSPI07G05010 vs. ExPASy TrEMBL
Match: A0A5A7SKE3 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003010 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.8e-148
Identity = 274/294 (93.20%), Postives = 279/294 (94.90%), Query Frame = 0

Query: 46  PNVPLTFPSRRQRRFKDGVVKSLTGIVSAVCRTASDLRWSVLKGKDEKTSIRSGREGLQF 105
           PNVPLTFPSRRQRRF +GVVKSLTGIVSAVCRTASDL WSV KG DEKT IRSGREGLQF
Sbjct: 163 PNVPLTFPSRRQRRFNNGVVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQF 222

Query: 106 MPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAATALTIV 165
           MPMNTFTVSFSLN IKKIKTKL VTVNDVITGI FMGLRLYMEE+EKNSGEVAATALTIV
Sbjct: 223 MPMNTFTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIV 282

Query: 166 NTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTN-TNPLEFVVAAHRSINNKRNS 225
           NTRVIG YR ATEMAKPESKGLWGNQLSYLE MIPKMTN TNPLEFV+AAHRSINNKR S
Sbjct: 283 NTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTS 342

Query: 226 FTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNHPISGLYFT 285
           FTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVI PLQQMALDNHPISGLYFT
Sbjct: 343 FTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFT 402

Query: 286 IVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKIFEAANDIP 339
           IVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAH+LN CMEDAF+KIFEAANDIP
Sbjct: 403 IVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456

BLAST of CSPI07G05010 vs. ExPASy TrEMBL
Match: A0A1S3C1V4 (Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495688 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.8e-148
Identity = 274/294 (93.20%), Postives = 279/294 (94.90%), Query Frame = 0

Query: 46  PNVPLTFPSRRQRRFKDGVVKSLTGIVSAVCRTASDLRWSVLKGKDEKTSIRSGREGLQF 105
           PNVPLTFPSRRQRRF +GVVKSLTGIVSAVCRTASDL WSV KG DEKT IRSGREGLQF
Sbjct: 163 PNVPLTFPSRRQRRFNNGVVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQF 222

Query: 106 MPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAATALTIV 165
           MPMNTFTVSFSLN IKKIKTKL VTVNDVITGI FMGLRLYMEE+EKNSGEVAATALTIV
Sbjct: 223 MPMNTFTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIV 282

Query: 166 NTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTN-TNPLEFVVAAHRSINNKRNS 225
           NTRVIG YR ATEMAKPESKGLWGNQLSYLE MIPKMTN TNPLEFV+AAHRSINNKR S
Sbjct: 283 NTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTS 342

Query: 226 FTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNHPISGLYFT 285
           FTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVI PLQQMALDNHPISGLYFT
Sbjct: 343 FTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFT 402

Query: 286 IVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKIFEAANDIP 339
           IVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAH+LN CMEDAF+KIFEAANDIP
Sbjct: 403 IVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456

BLAST of CSPI07G05010 vs. ExPASy TrEMBL
Match: A0A438JK81 (Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=WSD1_5 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 4.0e-73
Identity = 137/302 (45.36%), Postives = 206/302 (68.21%), Query Frame = 0

Query: 46  PNVPLTFPSRRQRRFKD---GVVKSLTGIVSAVCRTASDLRWSVLKG---KDEKTSIRSG 105
           PN+P+TFPS +     D    +++++   +SA   T SD  WS+LK    +D++T IRSG
Sbjct: 167 PNLPITFPSLKASSNLDKGRSIIRAVPRALSAAFNTVSDFGWSLLKSTVVEDDRTPIRSG 226

Query: 106 REGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAA 165
             G++F P++  T+SFSL+YI+KIK KL VT+NDV+TGI F G RLYM+ M   S    +
Sbjct: 227 DPGVEFRPLSIATISFSLDYIQKIKAKLGVTINDVLTGIIFFGTRLYMQSMNHASRNANS 286

Query: 166 TALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTN---TNPLEFVVAAHR 225
           TAL ++NTRVI  Y+   EM   +S   WGNQ ++L   +P++ +   T+PL+FV  A +
Sbjct: 287 TALVLLNTRVISGYKSLKEMTASDSSSQWGNQFAFLHVTLPELADAKFTSPLDFVAKAQQ 346

Query: 226 SINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNH 285
           +I  KRNS  +H+   LL  LRK RG +  A+++H  LKN++  I+N+I P +Q+ALDNH
Sbjct: 347 TIQRKRNSLAVHLTGRLLETLRKYRGPEVTARYIHGTLKNSSMAITNMIGPTEQVALDNH 406

Query: 286 PISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKIFEAAND 339
           P  G+YF ++G+P+S+T++++SYMGK+R+A  TEK FID  + N+C+E+AF+++FEAA  
Sbjct: 407 PCRGIYFMVLGSPESMTISIVSYMGKVRIAVGTEKGFIDPRKFNACIENAFQRVFEAALG 466

BLAST of CSPI07G05010 vs. ExPASy TrEMBL
Match: F6H5E6 (Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=VIT_12s0028g03480 PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 1.2e-72
Identity = 138/304 (45.39%), Postives = 205/304 (67.43%), Query Frame = 0

Query: 46  PNVPLTFPSRRQRRFKD---GVVKSLTGIVSAVCRTASDLRWSVLKG---KDEKTSIRSG 105
           PN+P+TFPS +     D    +++++   +SA   T SD  W +LK    +D++T IRSG
Sbjct: 167 PNLPITFPSFKVSSNLDSGRSIIRAVPRALSAAFNTVSDFGWGLLKSTAVEDDRTPIRSG 226

Query: 106 REGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAA 165
             G++F PM+  T+SFSL+ I+KIK KL VT+NDV+TGI F G RLYM+ M   S    +
Sbjct: 227 DPGVEFRPMSIATISFSLDNIQKIKAKLGVTINDVLTGIIFFGTRLYMQSMNHASRNANS 286

Query: 166 TALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTN---TNPLEFVVAAHR 225
           TAL ++NTRVI  Y+   EM   +S   WGNQ ++L   +P++ +   T+PL+FV  A +
Sbjct: 287 TALVLLNTRVISGYKSIKEMTASDSSSQWGNQFAFLHVTLPELADAKFTSPLDFVAKAQQ 346

Query: 226 SINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNH 285
           +I  KRNS  +H+   LL  LRK RG +  A+++H  LKN++  ISN+I P++Q+AL NH
Sbjct: 347 TIQRKRNSLAVHLTGRLLETLRKYRGPEVTARYIHGTLKNSSMTISNMIGPMEQLALANH 406

Query: 286 PISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKIFEAAND 341
           P  G+YF ++G+P+S+T+++LSYMGK+R+A  TEK FID  + N+C+E+AF+++FEAA  
Sbjct: 407 PCRGIYFMVLGSPESMTISILSYMGKVRIAVGTEKGFIDPRKFNACIENAFQRVFEAAVG 466

BLAST of CSPI07G05010 vs. ExPASy TrEMBL
Match: A0A6A6K6Y3 (WS_DGAT_C domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_043519 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 5.8e-72
Identity = 143/299 (47.83%), Postives = 204/299 (68.23%), Query Frame = 0

Query: 46  PNVPLTFPSRRQRRFKDGVVKSLTGIV----SAVCRTASDLRWSVLKG---KDEKTSIRS 105
           P++PLTFP   Q R K  + KS    V    S++  T SD  WS+LK    +D ++ +RS
Sbjct: 43  PSIPLTFPP-LQLRSKPEIFKSKKSTVSETFSSIFNTMSDFGWSLLKSSVVEDVQSPVRS 102

Query: 106 GREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVA 165
           G +G++F P+   T++FSL+ IK+IKT+L VT+NDVITGI F G+RLYM  +  NS    
Sbjct: 103 GDDGVEFKPITISTMTFSLDQIKQIKTRLGVTINDVITGIIFYGIRLYMLAVSNNSTNAH 162

Query: 166 ATALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMT---NTNPLEFVVAAH 225
           +TAL ++NTR+IG+Y+   EM KP ++  WGNQ  +L   +P++T    TNPL FV  A 
Sbjct: 163 STALVLLNTRIIGSYKSVKEMVKPNAESPWGNQFGFLHVSMPELTKAAETNPLLFVEKAQ 222

Query: 226 RSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDN 285
           + I  KR S  +++   LL  +RKLRG +  AK++H+ LKN++  ISNVI P+++MAL N
Sbjct: 223 QIIKRKRGSLAVNLTGKLLEAVRKLRGPEATAKYIHSTLKNSSMTISNVIGPVERMALAN 282

Query: 286 HPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKIFEAA 335
           HP+ GLYF +VG PQS+T+TM+SY GKLR+A  TEKDFID  +  SC+E+AF+ IF+++
Sbjct: 283 HPVKGLYFMVVGVPQSLTITMVSYTGKLRIAVGTEKDFIDPQKFKSCIENAFEMIFKSS 340

BLAST of CSPI07G05010 vs. NCBI nr
Match: XP_008455536.1 (PREDICTED: O-acyltransferase WSD1-like [Cucumis melo] >KAA0031532.1 O-acyltransferase WSD1-like [Cucumis melo var. makuwa] >TYK06985.1 O-acyltransferase WSD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 534.3 bits (1375), Expect = 7.9e-148
Identity = 274/294 (93.20%), Postives = 279/294 (94.90%), Query Frame = 0

Query: 46  PNVPLTFPSRRQRRFKDGVVKSLTGIVSAVCRTASDLRWSVLKGKDEKTSIRSGREGLQF 105
           PNVPLTFPSRRQRRF +GVVKSLTGIVSAVCRTASDL WSV KG DEKT IRSGREGLQF
Sbjct: 163 PNVPLTFPSRRQRRFNNGVVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQF 222

Query: 106 MPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAATALTIV 165
           MPMNTFTVSFSLN IKKIKTKL VTVNDVITGI FMGLRLYMEE+EKNSGEVAATALTIV
Sbjct: 223 MPMNTFTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIV 282

Query: 166 NTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTN-TNPLEFVVAAHRSINNKRNS 225
           NTRVIG YR ATEMAKPESKGLWGNQLSYLE MIPKMTN TNPLEFV+AAHRSINNKR S
Sbjct: 283 NTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTS 342

Query: 226 FTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNHPISGLYFT 285
           FTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVI PLQQMALDNHPISGLYFT
Sbjct: 343 FTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFT 402

Query: 286 IVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKIFEAANDIP 339
           IVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAH+LN CMEDAF+KIFEAANDIP
Sbjct: 403 IVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456

BLAST of CSPI07G05010 vs. NCBI nr
Match: XP_004137035.2 (uncharacterized protein LOC101219464 [Cucumis sativus])

HSP 1 Score: 286.2 bits (731), Expect = 3.7e-73
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 0

Query: 198 MIPKMTNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNT 257
           MIPKMTNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNT
Sbjct: 1   MIPKMTNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNT 60

Query: 258 TTVISNVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAH 317
           TTVISNVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAH
Sbjct: 61  TTVISNVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAH 120

Query: 318 ELNSCMEDAFKKIFEAANDIPSP 341
           ELNSCMEDAFKKIFEAANDIPSP
Sbjct: 121 ELNSCMEDAFKKIFEAANDIPSP 143

BLAST of CSPI07G05010 vs. NCBI nr
Match: RVX09350.1 (O-acyltransferase WSD1 [Vitis vinifera])

HSP 1 Score: 285.0 bits (728), Expect = 8.3e-73
Identity = 137/302 (45.36%), Postives = 206/302 (68.21%), Query Frame = 0

Query: 46  PNVPLTFPSRRQRRFKD---GVVKSLTGIVSAVCRTASDLRWSVLKG---KDEKTSIRSG 105
           PN+P+TFPS +     D    +++++   +SA   T SD  WS+LK    +D++T IRSG
Sbjct: 167 PNLPITFPSLKASSNLDKGRSIIRAVPRALSAAFNTVSDFGWSLLKSTVVEDDRTPIRSG 226

Query: 106 REGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAA 165
             G++F P++  T+SFSL+YI+KIK KL VT+NDV+TGI F G RLYM+ M   S    +
Sbjct: 227 DPGVEFRPLSIATISFSLDYIQKIKAKLGVTINDVLTGIIFFGTRLYMQSMNHASRNANS 286

Query: 166 TALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTN---TNPLEFVVAAHR 225
           TAL ++NTRVI  Y+   EM   +S   WGNQ ++L   +P++ +   T+PL+FV  A +
Sbjct: 287 TALVLLNTRVISGYKSLKEMTASDSSSQWGNQFAFLHVTLPELADAKFTSPLDFVAKAQQ 346

Query: 226 SINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNH 285
           +I  KRNS  +H+   LL  LRK RG +  A+++H  LKN++  I+N+I P +Q+ALDNH
Sbjct: 347 TIQRKRNSLAVHLTGRLLETLRKYRGPEVTARYIHGTLKNSSMAITNMIGPTEQVALDNH 406

Query: 286 PISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKIFEAAND 339
           P  G+YF ++G+P+S+T++++SYMGK+R+A  TEK FID  + N+C+E+AF+++FEAA  
Sbjct: 407 PCRGIYFMVLGSPESMTISIVSYMGKVRIAVGTEKGFIDPRKFNACIENAFQRVFEAALG 466

BLAST of CSPI07G05010 vs. NCBI nr
Match: XP_034680664.1 (uncharacterized protein LOC117910661 [Vitis riparia])

HSP 1 Score: 283.9 bits (725), Expect = 1.9e-72
Identity = 139/304 (45.72%), Postives = 205/304 (67.43%), Query Frame = 0

Query: 46  PNVPLTFPSRRQRRFKD---GVVKSLTGIVSAVCRTASDLRWSVLKG---KDEKTSIRSG 105
           PN+P+TFPS +     D    +++++   +SA   T SD  WS+LK    +D++T IRSG
Sbjct: 14  PNLPITFPSFKVSSNLDRGRSIIRAVPRALSAAFNTVSDFGWSLLKSTAMEDDRTPIRSG 73

Query: 106 REGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAA 165
             G++F PM+  T+SFSL+ I+KIK KL VT+NDV+TGI F G RLYM+ M   S    +
Sbjct: 74  DPGVEFRPMSIATISFSLDNIQKIKAKLGVTINDVLTGIIFFGTRLYMQSMNHASRNANS 133

Query: 166 TALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTN---TNPLEFVVAAHR 225
           TAL ++NTRVI  Y+   EM   +S   WGNQ ++L   +P++ +   T+PL+FV  A +
Sbjct: 134 TALVLLNTRVISGYKSIKEMTASDSSSQWGNQFAFLHVTLPELADAKFTSPLDFVAKAQQ 193

Query: 226 SINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNH 285
           +I  KRNS  +H+   LL  LRK RG +  A+++H+ LKN++  ISN+I P++Q+AL NH
Sbjct: 194 TIQRKRNSLAVHLTGRLLETLRKYRGPEVTARYIHSTLKNSSMTISNMIGPMEQVALANH 253

Query: 286 PISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKIFEAAND 341
           P  G+YF  +G+P+S+T+++LSYMGK+R+   TEK FID  + N+C+EDAF+++FEAA  
Sbjct: 254 PCRGMYFITLGSPESMTISILSYMGKVRITVGTEKGFIDPRKFNACIEDAFQRVFEAAVG 313

BLAST of CSPI07G05010 vs. NCBI nr
Match: CBI21867.3 (unnamed protein product, partial [Vitis vinifera])

HSP 1 Score: 283.5 bits (724), Expect = 2.4e-72
Identity = 138/304 (45.39%), Postives = 205/304 (67.43%), Query Frame = 0

Query: 46  PNVPLTFPSRRQRRFKD---GVVKSLTGIVSAVCRTASDLRWSVLKG---KDEKTSIRSG 105
           PN+P+TFPS +     D    +++++   +SA   T SD  W +LK    +D++T IRSG
Sbjct: 136 PNLPITFPSFKVSSNLDSGRSIIRAVPRALSAAFNTVSDFGWGLLKSTAVEDDRTPIRSG 195

Query: 106 REGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAA 165
             G++F PM+  T+SFSL+ I+KIK KL VT+NDV+TGI F G RLYM+ M   S    +
Sbjct: 196 DPGVEFRPMSIATISFSLDNIQKIKAKLGVTINDVLTGIIFFGTRLYMQSMNHASRNANS 255

Query: 166 TALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTN---TNPLEFVVAAHR 225
           TAL ++NTRVI  Y+   EM   +S   WGNQ ++L   +P++ +   T+PL+FV  A +
Sbjct: 256 TALVLLNTRVISGYKSIKEMTASDSSSQWGNQFAFLHVTLPELADAKFTSPLDFVAKAQQ 315

Query: 226 SINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNH 285
           +I  KRNS  +H+   LL  LRK RG +  A+++H  LKN++  ISN+I P++Q+AL NH
Sbjct: 316 TIQRKRNSLAVHLTGRLLETLRKYRGPEVTARYIHGTLKNSSMTISNMIGPMEQLALANH 375

Query: 286 PISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKIFEAAND 341
           P  G+YF ++G+P+S+T+++LSYMGK+R+A  TEK FID  + N+C+E+AF+++FEAA  
Sbjct: 376 PCRGIYFMVLGSPESMTISILSYMGKVRIAVGTEKGFIDPRKFNACIENAFQRVFEAAVG 435

BLAST of CSPI07G05010 vs. TAIR 10
Match: AT5G22490.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 105.5 bits (262), Expect = 8.4e-23
Identity = 68/231 (29.44%), Postives = 114/231 (49.35%), Query Frame = 0

Query: 116 SLNYIKKIKTKLDVTVNDVITGITFMGLRLYMEEMEKNSGEVAATALT------------ 175
           SL+ IK IK  +++T+NDV+ G+T   L  Y+ +    + E A T+LT            
Sbjct: 239 SLDDIKLIKNAMEMTINDVLLGVTEAALTRYLHQSYDKTNEEAGTSLTPNRQDLLDRIRL 298

Query: 176 ----IVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKMTNTNPLEFVVAAHRSIN 235
               +VN R  G+   A  MAK  SK  WGN +S +         ++PL ++      I+
Sbjct: 299 RSLIVVNLRPTGSQSIADMMAK-GSKCRWGNYISVILFPFTIALQSDPLVYLSNVKSMID 358

Query: 236 NKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNHPIS 295
            K+NS   +++      + K  G      F   ++ NTT  ISN+  P ++++   HPI+
Sbjct: 359 RKKNSLITYIIYTFSEFVIKAFGINVAVAFQRKIMLNTTMCISNLPGPTEEVSFHGHPIA 418

Query: 296 GLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFIDAHELNSCMEDAFKKI 331
               +I G PQ++T+  LSY  K+ ++   +   IDAH+L   +E++ K +
Sbjct: 419 YFAPSIYGLPQALTIHYLSYANKMIISVAVDPMIIDAHKLCDELEESLKNM 468

BLAST of CSPI07G05010 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 100.1 bits (248), Expect = 3.5e-21
Identity = 67/253 (26.48%), Postives = 120/253 (47.43%), Query Frame = 0

Query: 90  KDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGL-----R 149
           KD +T I+      +   +     + SL+ IK  K  +++T+NDV+ G+T  GL     R
Sbjct: 232 KDTETPIKGDFLSTKSKQLRLVHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLAR 291

Query: 150 LYMEEMEKNSGE------VAATALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETM 209
            Y EE  KN  +      +   +  +VN R     +   +M +  SK  WGN   Y+   
Sbjct: 292 RYGEEETKNRKQKKLPKRIRLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFP 351

Query: 210 IPKMTNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTT 269
                  +PLE +  A ++I+ K+NS+   +      ++ KL G +  A  ++ ++ NTT
Sbjct: 352 FSIALRDDPLEHLEIAQKTISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTT 411

Query: 270 TVISNVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFI-DAH 329
              SN++ P+++++   HPI+    +  G P ++T+   SYM K+ +    +   I D H
Sbjct: 412 MTFSNMVGPVEEVSFYGHPITYFASSAYGHPHALTIHCQSYMNKMTITLIVDPTVISDPH 471

Query: 330 ELNSCMEDAFKKI 331
            L    E++ + I
Sbjct: 472 RLCDDWEESLRSI 484

BLAST of CSPI07G05010 vs. TAIR 10
Match: AT5G12420.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-20
Identity = 68/260 (26.15%), Postives = 131/260 (50.38%), Query Frame = 0

Query: 85  SVLKGKDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLR 144
           +VL  KD KT +++G + ++  P   +    SL+ IK IK  +++T+NDV+ GIT   L 
Sbjct: 210 TVLFLKDTKTPLKAGAD-VRSNPKRFYHRIISLDDIKLIKNAMNMTINDVLFGITQASLS 269

Query: 145 LYMEEM--EKNSGEVAATA-------------LTIVNTRVIGTYRPATEMAKPESKGLWG 204
            Y+      K   + A T+                VN R    ++P  +M   +SK  WG
Sbjct: 270 HYLNRQYGTKKEEDGALTSYRNNLPDGIRFRVACTVNLRSDIGFKPLADMMVKDSKCRWG 329

Query: 205 NQLSYLETMIPKMTNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKF 264
           N  S++         T+PL ++  +   +  K++S+   +V  ++ ++ K+ G +  A+ 
Sbjct: 330 NYFSFIFLPFTIGLQTDPLVYLKMSKSMMARKKHSYHAALVYFIIKIVLKVFGAKAAAEL 389

Query: 265 LHNLLKNTTTVISNVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRT 324
               ++NTTT +SNVI P+++++   HP+S +  +  G   ++ + ++SY  K+ ++   
Sbjct: 390 FDRPVRNTTTCVSNVIGPMEEISFRGHPVSYIAPSSYGHSHALLIHLMSYADKMIISLAY 449

Query: 325 EKDFI-DAHELNSCMEDAFK 329
           +   I D H++   ME++ K
Sbjct: 450 DPTVISDPHKICDDMEESLK 468

BLAST of CSPI07G05010 vs. TAIR 10
Match: AT5G16350.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 95.5 bits (236), Expect = 8.7e-20
Identity = 74/307 (24.10%), Postives = 140/307 (45.60%), Query Frame = 0

Query: 53  PSRRQRRFKDGVVKSLTGIVSAVCRTASDLR--WSVLKG-----------KDEKTSIRSG 112
           PS ++R      +K     ++A+    S +R  W+ L             KD KT ++ G
Sbjct: 165 PSVKRRSTVSHSLKKTGWFLTAIFTIGSTMRLIWNTLVDMFLLFATMLFLKDTKTPLKGG 224

Query: 113 REGLQFMPMNTFTVSFSLNYIKKIKTKLDVTVNDVITGITFMGLRLYME---EMEKNSGE 172
              ++  P   +  + SL+ IK IK  +++T+NDV+ GIT   L  Y+    E E N+ E
Sbjct: 225 -ANVRSNPKTFYHRNISLDDIKLIKNAMNMTINDVLLGITQAALSSYLNRRYEHENNNEE 284

Query: 173 --------------VAATALTIVNTRVIGTYRPATEMAKPESKGLWGNQLSYLETMIPKM 232
                         +   A   VN R    ++P  EM   +SK  WGN  S++   +   
Sbjct: 285 DGVLTSYTNNLPDRIRFRAGCTVNLRSDIGFKPLAEMMVKDSKCRWGNYFSFIILPLSIS 344

Query: 233 TNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVIS 292
             T+PL ++  +   +   ++S+   +   L+ +  K+ G +      +  L N TT +S
Sbjct: 345 LETDPLVYLNKSKAMMARTKHSYQAALTYFLIKISLKVLGAKATTSLFNQHLMNITTCVS 404

Query: 293 NVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGKLRVAFRTEKDFI-DAHELNS 329
           NV+ P+++++ + HP++ +  +  G   ++ +   SY G++ +    +   I D H++  
Sbjct: 405 NVMGPMEEISFNGHPVAYISPSSYGHSHALLIHYTSYAGEMTITITVDPTVIPDPHKICD 464

BLAST of CSPI07G05010 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-19
Identity = 73/272 (26.84%), Postives = 130/272 (47.79%), Query Frame = 0

Query: 71  IVSAVCRTASDLRWSVLKGKDEKTSIRSGREGLQFMPMNTFTVSFSLNYIKKIKTKLDVT 130
           +++ VC  A +   + L  KD +T I+   +  +   M     + SL+ IK IK  + +T
Sbjct: 226 VLNTVC-DALEFIATALFLKDTETPIKGDFKLSKGKRMCMVHRTVSLDDIKLIKNAMKMT 285

Query: 131 VNDVITGITFMGLRLYM-------EEMEKNSGE----VAATALTIVNTRVIGTYRPATEM 190
           VNDV+ G++  GL  Y+       EE ++NS      +   A  +VN R     +   +M
Sbjct: 286 VNDVVLGVSQAGLSQYLKRRYGEQEESKRNSSNIPKGIRLRAALLVNLRPTTGIQDLADM 345

Query: 191 AKPESKGLWGNQLSYLETMIPKMTNTNPLEFVVAAHRSINNKRNSFTIHVVAHLLNLLRK 250
               SK  WGN + Y+          +PL+ +  A  +I+ K+NS    +   +  +L  
Sbjct: 346 MTKGSKCRWGNWIGYIIFPFSIALCDDPLKHLRRAKSTIDRKKNSLEAVLTFVVGKILLN 405

Query: 251 LRGHQEVAKFLHNLLKNTTTVISNVIDPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSY 310
             G Q  A  L+  L NTT   SN++ P+++++   H ++ +  ++ G P ++T+   SY
Sbjct: 406 TLGVQRAANVLNRALSNTTMSFSNLVGPVEEISFYGHTVTYIAPSVYGHPHALTMHFQSY 465

Query: 311 MGKLRVAFRTEKDFI-DAHELNSCMEDAFKKI 331
           M KL ++   +   I D H+L    E++ + I
Sbjct: 466 MNKLTISLTVDPTVISDPHKLCDDWEESLRSI 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FK891.2e-2129.44Wax ester synthase/diacylglycerol acyltransferase 9 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B35.0e-2026.48Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3... [more]
Q94CK02.5e-1926.15Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3... [more]
Q9FFE81.2e-1824.10Wax ester synthase/diacylglycerol acyltransferase 8 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B21.6e-1826.84Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5A7SKE33.8e-14893.20Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C1V43.8e-14893.20Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495688 PE=4 SV... [more]
A0A438JK814.0e-7345.36Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=WSD1_5 PE=4 SV=1[more]
F6H5E61.2e-7245.39Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=VIT_12s0028g03480... [more]
A0A6A6K6Y35.8e-7247.83WS_DGAT_C domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_04351... [more]
Match NameE-valueIdentityDescription
XP_008455536.17.9e-14893.20PREDICTED: O-acyltransferase WSD1-like [Cucumis melo] >KAA0031532.1 O-acyltransf... [more]
XP_004137035.23.7e-73100.00uncharacterized protein LOC101219464 [Cucumis sativus][more]
RVX09350.18.3e-7345.36O-acyltransferase WSD1 [Vitis vinifera][more]
XP_034680664.11.9e-7245.72uncharacterized protein LOC117910661 [Vitis riparia][more]
CBI21867.32.4e-7245.39unnamed protein product, partial [Vitis vinifera][more]
Match NameE-valueIdentityDescription
AT5G22490.18.4e-2329.44O-acyltransferase (WSD1-like) family protein [more]
AT3G49190.13.5e-2126.48O-acyltransferase (WSD1-like) family protein [more]
AT5G12420.11.8e-2026.15O-acyltransferase (WSD1-like) family protein [more]
AT5G16350.18.7e-2024.10O-acyltransferase (WSD1-like) family protein [more]
AT3G49200.11.1e-1926.84O-acyltransferase (WSD1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009721O-acyltransferase WSD1, C-terminalPFAMPF06974WS_DGAT_Ccoord: 188..330
e-value: 1.3E-34
score: 119.3
IPR045034O-acyltransferase WSD1-likePANTHERPTHR31650O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 46..337
NoneNo IPR availablePANTHERPTHR31650:SF34O-ACYLTRANSFERASE WSD1-LIKEcoord: 46..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G05010.1CSPI07G05010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0045017 glycerolipid biosynthetic process
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity
molecular_function GO:0047196 long-chain-alcohol O-fatty-acyltransferase activity
molecular_function GO:0008374 O-acyltransferase activity