CSPI07G01730 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G01730
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionProtein kinase domain-containing protein
LocationChr7: 1516309 .. 1520559 (-)
RNA-Seq ExpressionCSPI07G01730
SyntenyCSPI07G01730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGAATTCAATTTTGCACGTAGTTAGATAGATTGAAACCAAACAACCATCATGCACGGCAGTTGACTTTGAACAAACAAATAGTTTTTCAGTCTGTTGTCATCTTCTTCAGTCCCCACCAGGTTTCCTTTCTTTTAAAATTTCTGCGACTCATTCTTCCCGGTAAAATCCTTCAATCTTCAATCTAAACACCCCCTTTGTCTACTCCCCTGCTTTTTCACCGCAATTGATTTCAATAGCTACTGACATTTCTGCGTATTTCCCCTTAAATTCGTGTTCATTGGATTATATTTGTGATAATTGGGTGATTACTGAATTAATTGGTCAAGAAATGTACAGGTGGGATGAGGGATAAATGATATAGTTGACTGACTAGTCTGGATTGATTTTATTTTATTTCACAAGGGCTCTCCTCAATTATCTTCTTCTTCTTCTTCTTCTTCTTCTCTCTTTTTGTGCTCTCAACAGGAAGAGGGAGAGATAGAGAAGATAAATGGGGATTTGTTGGGGTTCTCCAGTTGATAATCATACTCCTAGTACCACTGGACATTTCAGTTCAGGTAATTTCAGCAGGTTCTACTTGAATCCAGTTCTATTTTAAAGTTTGTGCTTGATATCTGCAAGTTTTCCCAAATTATTTTTCCCCCTTTAGAGTTTGGGGTTCTTTGATGGGGTTCTTTTGGTTAAACTTCTGAGTTATTTATGTTTTATATCGTTTGTCACTCTTTGTGGAGGAAATATTTTCTGGAACTCTTTTCTACTCTTCCCATTTGGGTACTTGTTTGTTTCATGTTTGGAGTTTTCTAGCTTTTTAGGTGTTTGTGTGGTTGGTTTTTCCATTGAATTTTTAGCTGTATATATTGTTTGTTGTTTATCAGTCTTTGCAGAGGAAATACTTTTTGAAACTCTTTTCCACTCCACTTATGGGTAATTTTTTTGTTTCATCTGTTTGGAGTTTCATAGCTTTTGAAGTGTGTGGTTGGCTTTTCATTGAATTTTGAGGTATAGAAGTTGTATATTTTTTATCAGTCTTTGGGGAGGAATTACTTTTTGAAACTCTTTTCCATCCTTCCCATGTGGGTAATTGTTTGTTTCATGTTTGGAGTTTCCTAGCTTTTTGAGTGTACGTGGTTGGTTTTTCCATCGAATTTTGAGTCATATATGTTGTGTATTGTTTATCAGTCTTTGATGAGGAAATACTTTTTGAAACACTTTTCCACTCTTTCCAAATGGGTATTTGTTTGTTTCATGTTTGATATTTCCTAGCTTTTTAAGTGTATGTGGTTGATTTTTCCATTGAAGTTTGATCCGAGACTAAGAGCAACATGCAGATAATGATTATATTATTTACGATTTGGAAAAATTTCCCTTATTTATATGATTCAAACGTGTGGCTATAACAGTTATATCCCAGACAACCAGTCTGACAACATCATCAACTATCAGCAACATCTCCAAGAACAGTCAATTCTCTGCAGCAAGTGGAGATGAAGTTTTTCCACATGGACAGATTTTGCCCTCTTCGAACTTGAGGGAGTATAGCTTGGCAGAGCTCAAAGCTGCAACCAAAAACTTCAGAGCTGAAGCATTGCTTGGTGAAGGAGGTTTTGGCAAGGTTTACAAAGGTTGGCTCGAGGAGAAGGGCCTTGGAAGGAAGGGAAATTCGATGGTTATTGCGGTAAAAAAGTTGAAATCTGACAGTGTGCAAGGAATTGAGGAGTGGCAGGTAAATTATATAGTTATAGAGAAGAATACTCTCTTCCTTAAAATCTCCTCGAACTATGGTAGGGAGAAACTTTTCTATTAAGAGATTGCTCACCCTGTGTCCTGATTCCAAGCTACTAGGTTGAAAACTAAATAACTTCAAGTTCTTGTCTTAGATCTGGCCTTAGAATGAGCTAAGCCTCTCTGAGGGTTCATGGAGTGAACCATGAGCATAAATCAAAGAGTGAGGTCTGAAACACCCACCTATTTAAAGGAATAAATAAATAAAAATCATGGGTGGACTCAAATTTGAATTACTTGAGTGATGTTTAAGAACAACTCAACTGAAAACATTTAAGAAAAAGACACCAACTACATAGATTTTAAGTATTGAAGAATTGACAAAGTATGTTTACACCATTTCGAAACAACACCTGAATGTGATATCCATCAGTCATGTTTCACCGTACTGCTACCTCTGGCAGTGGCAACTACCATCAATAATTTTTAAACTAGGCAAGATTGAGCTACCAGAATTGTTAACTAGATGGAACAGTATGAATTGCTCTATGAACGAGATGGGACAATATGAACTACGTAGGCTGTGTTTGAAACTTTTATGATTTCCTATCTTTATCTTCTTAGCATGTGTTAGTGTATAAACATATAAACTTGTTCAAGACTACCATCAGAACTGTCCATCCGTAAGTTGATCAATTTGTTACCGACTTTGTAGTGACAATGACTGTTGCATATTCCAGAAGTTTCCTTCTCAAGATTTCTTGTTCAAGACTACCATCAGAACTGTCCATCCGTAAGTTGATCAATTTGTTACCGACTTTGTAGTGACAATGACTGTTGCATATTCCAGAAGTTTCCTTCTCAAGATTTTTTTTTTTTTTTGAAAAAATGGACATATAACAACATAGTATGGTTGGTTATGCAGTCAGAAGTGGGCTTCTTGGGAAGACTTTCTCATCCAAATCTAGTGAAACTATTGGGTTATTGTTGGGAGGATCACGAGTTACTCCTTACTTATGAGTTCATGCAAAAAGGAAGCTTGGAGAACCATCTATTTGGAAGTAAGAAAACTAATTGATGATATTATATACTATGATGTTTCTTTCAAATAATTGCAGTGTTCTTAGCTTTGTTTACTGAAGTGTAAACAACATTTCTGTGAAATTCAGGAGGATCGGCAGTTACGCCACTCGGATGGGATACTCGGCTTAAGATCGCTATAGGTGCTGCTCGAGGGCTAGCGTTCTTGCACACATCAGACAAGCAAGTAATTTATAGAGATTTCAAGGCTTCTAATATTCTGCTTGATGGGGTAAAGTAATATTTATTATGAATCTACACATAAATATACACATTAAGAAAGAGTCTTATACTAATCTCAATCTTTCATTGACAAACTTTTTTATGATCATCTTTTCCTTTTTATATCTAATCTCCTTGTATTTGTCGTAACCCCAATATTCCTTTACCTGCAGTCATATACAGCAAAGTTATCCGACTTTGGCTTGGCGAAATTGGGTCCTTCAGAAAGCAAATCTCATCTGACGACTCGAGTTATGGGCACACATGGTTATGCTGCTCCAGAATACGTTACCACAGGTAGAACAAAAACTTTAAAACTTTTCAAAATTGGCTGTCTTTGTTGAAGGAGTCAATTTTAATTCCAAAACTGGAGCTTATATCTCCAAGCAATTTTCAAGTTGAAATTAATGATGATATTTATGACACAGGACATCTGTATGTCAAGAGCGATGTATATGGTTTTGGAGTCGTATTGATTGAAATGTTGACTGGTTTACGTGCTCTCGACGAAAATCGTCCAACTGGGCAAGAACACCTCACAGAATGGATAAAACCATTCTTATCAGAGAGAAGGAAGCTGAAAAACGTGATGGACTTCCGGTTGGAAGGAAAATATCCATCCAGATCTGCATTCCAAGTGGCTCAGCTAGCTCTACAATGTATTGAGCAAGAGCAGAAAAACCGACCATCGATGAAAGAGGTGGTCGAGACATTGGAACAAATCGAAACTGTGAACGAGAAACTGATCGAGACTGGTAACCGTGCTTCCCGTCTGGCATCAAATCGCAATGCTCATCAGCCACTTCACCATAATTCTCCACTTCACATGAAGCAATATGGAGGCCATGCTAATCAAACACCACCCAGATGCCGATAGATTCTCTTATCCCACTCTGATTTCTTCATTCATATGTTTCTTTAGAGATGTTAGTTTGAAAGTTCATTTTCTCAGTTGTAAGATTGTAATTAAGTACTTCTTAGGTTGTGGATGTTGGGAGTTAGGACAGTTATTGCATGACACTGGATTGTCAAGTTTGTTTGATCTTTGAAGTCCAGCTTTTCTTGTTTTCTTTTGTTAGTAAAACTTAAATTGTTTTATCTGTACAAGAAACTAAGTGGCAAAACTGATGAAGCCATATTGTCTTTTGTAATTAGAGAGAGAAAGAGAGAGAGAGAGGTTATAACCTAATTATTTTGATTCAATACACAATTTACAAATCCGCTC

mRNA sequence

TTGAATTCAATTTTGCACGTAGTTAGATAGATTGAAACCAAACAACCATCATGCACGGCAGTTGACTTTGAACAAACAAATAGTTTTTCAGTCTGTTGTCATCTTCTTCAGTCCCCACCAGGTTTCCTTTCTTTTAAAATTTCTGCGACTCATTCTTCCCGGTAAAATCCTTCAATCTTCAATCTAAACACCCCCTTTGTCTACTCCCCTGCTTTTTCACCGCAATTGATTTCAATAGCTACTGACATTTCTGCGTATTTCCCCTTAAATTCGTGTTCATTGGATTATATTTGTGATAATTGGGTGATTACTGAATTAATTGGTCAAGAAATGTACAGGTGGGATGAGGGATAAATGATATAGTTGACTGACTAGTCTGGATTGATTTTATTTTATTTCACAAGGGCTCTCCTCAATTATCTTCTTCTTCTTCTTCTTCTTCTTCTCTCTTTTTGTGCTCTCAACAGGAAGAGGGAGAGATAGAGAAGATAAATGGGGATTTGTTGGGGTTCTCCAGTTGATAATCATACTCCTAGTACCACTGGACATTTCAGTTCAGTTATATCCCAGACAACCAGTCTGACAACATCATCAACTATCAGCAACATCTCCAAGAACAGTCAATTCTCTGCAGCAAGTGGAGATGAAGTTTTTCCACATGGACAGATTTTGCCCTCTTCGAACTTGAGGGAGTATAGCTTGGCAGAGCTCAAAGCTGCAACCAAAAACTTCAGAGCTGAAGCATTGCTTGGTGAAGGAGGTTTTGGCAAGGTTTACAAAGGTTGGCTCGAGGAGAAGGGCCTTGGAAGGAAGGGAAATTCGATGGTTATTGCGGTAAAAAAGTTGAAATCTGACAGTGTGCAAGGAATTGAGGAGTGGCAGTCAGAAGTGGGCTTCTTGGGAAGACTTTCTCATCCAAATCTAGTGAAACTATTGGGTTATTGTTGGGAGGATCACGAGTTACTCCTTACTTATGAGTTCATGCAAAAAGGAAGCTTGGAGAACCATCTATTTGGAAGAGGATCGGCAGTTACGCCACTCGGATGGGATACTCGGCTTAAGATCGCTATAGGTGCTGCTCGAGGGCTAGCGTTCTTGCACACATCAGACAAGCAAGTAATTTATAGAGATTTCAAGGCTTCTAATATTCTGCTTGATGGGTCATATACAGCAAAGTTATCCGACTTTGGCTTGGCGAAATTGGGTCCTTCAGAAAGCAAATCTCATCTGACGACTCGAGTTATGGGCACACATGGTTATGCTGCTCCAGAATACGTTACCACAGGACATCTGTATGTCAAGAGCGATGTATATGGTTTTGGAGTCGTATTGATTGAAATGTTGACTGGTTTACGTGCTCTCGACGAAAATCGTCCAACTGGGCAAGAACACCTCACAGAATGGATAAAACCATTCTTATCAGAGAGAAGGAAGCTGAAAAACGTGATGGACTTCCGGTTGGAAGGAAAATATCCATCCAGATCTGCATTCCAAGTGGCTCAGCTAGCTCTACAATGTATTGAGCAAGAGCAGAAAAACCGACCATCGATGAAAGAGGTGGTCGAGACATTGGAACAAATCGAAACTGTGAACGAGAAACTGATCGAGACTGGTAACCGTGCTTCCCGTCTGGCATCAAATCGCAATGCTCATCAGCCACTTCACCATAATTCTCCACTTCACATGAAGCAATATGGAGGCCATGCTAATCAAACACCACCCAGATGCCGATAGATTCTCTTATCCCACTCTGATTTCTTCATTCATATGTTTCTTTAGAGATGTTAGTTTGAAAGTTCATTTTCTCAGTTGTAAGATTGTAATTAAGTACTTCTTAGGTTGTGGATGTTGGGAGTTAGGACAGTTATTGCATGACACTGGATTGTCAAGTTTGTTTGATCTTTGAAGTCCAGCTTTTCTTGTTTTCTTTTGTTAGTAAAACTTAAATTGTTTTATCTGTACAAGAAACTAAGTGGCAAAACTGATGAAGCCATATTGTCTTTTGTAATTAGAGAGAGAAAGAGAGAGAGAGAGGTTATAACCTAATTATTTTGATTCAATACACAATTTACAAATCCGCTC

Coding sequence (CDS)

ATGGGGATTTGTTGGGGTTCTCCAGTTGATAATCATACTCCTAGTACCACTGGACATTTCAGTTCAGTTATATCCCAGACAACCAGTCTGACAACATCATCAACTATCAGCAACATCTCCAAGAACAGTCAATTCTCTGCAGCAAGTGGAGATGAAGTTTTTCCACATGGACAGATTTTGCCCTCTTCGAACTTGAGGGAGTATAGCTTGGCAGAGCTCAAAGCTGCAACCAAAAACTTCAGAGCTGAAGCATTGCTTGGTGAAGGAGGTTTTGGCAAGGTTTACAAAGGTTGGCTCGAGGAGAAGGGCCTTGGAAGGAAGGGAAATTCGATGGTTATTGCGGTAAAAAAGTTGAAATCTGACAGTGTGCAAGGAATTGAGGAGTGGCAGTCAGAAGTGGGCTTCTTGGGAAGACTTTCTCATCCAAATCTAGTGAAACTATTGGGTTATTGTTGGGAGGATCACGAGTTACTCCTTACTTATGAGTTCATGCAAAAAGGAAGCTTGGAGAACCATCTATTTGGAAGAGGATCGGCAGTTACGCCACTCGGATGGGATACTCGGCTTAAGATCGCTATAGGTGCTGCTCGAGGGCTAGCGTTCTTGCACACATCAGACAAGCAAGTAATTTATAGAGATTTCAAGGCTTCTAATATTCTGCTTGATGGGTCATATACAGCAAAGTTATCCGACTTTGGCTTGGCGAAATTGGGTCCTTCAGAAAGCAAATCTCATCTGACGACTCGAGTTATGGGCACACATGGTTATGCTGCTCCAGAATACGTTACCACAGGACATCTGTATGTCAAGAGCGATGTATATGGTTTTGGAGTCGTATTGATTGAAATGTTGACTGGTTTACGTGCTCTCGACGAAAATCGTCCAACTGGGCAAGAACACCTCACAGAATGGATAAAACCATTCTTATCAGAGAGAAGGAAGCTGAAAAACGTGATGGACTTCCGGTTGGAAGGAAAATATCCATCCAGATCTGCATTCCAAGTGGCTCAGCTAGCTCTACAATGTATTGAGCAAGAGCAGAAAAACCGACCATCGATGAAAGAGGTGGTCGAGACATTGGAACAAATCGAAACTGTGAACGAGAAACTGATCGAGACTGGTAACCGTGCTTCCCGTCTGGCATCAAATCGCAATGCTCATCAGCCACTTCACCATAATTCTCCACTTCACATGAAGCAATATGGAGGCCATGCTAATCAAACACCACCCAGATGCCGATAG

Protein sequence

MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQILPSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKSDSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAVTPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEHLTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETLEQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR*
Homology
BLAST of CSPI07G01730 vs. ExPASy Swiss-Prot
Match: Q9SII6 (Probable serine/threonine-protein kinase PIX13 OS=Arabidopsis thaliana OX=3702 GN=PIX13 PE=1 SV=2)

HSP 1 Score: 540.0 bits (1390), Expect = 2.3e-152
Identity = 279/395 (70.63%), Postives = 321/395 (81.27%), Query Frame = 0

Query: 1   MGICWGSPVDN---HTPSTTGHFSSV----ISQTTSLTTSSTISNISKNSQFSAASGDEV 60
           MG+CWGSP D+    TPS+TG+ SSV     S  T+ T +S  SNIS NS FS ASG++ 
Sbjct: 1   MGLCWGSPSDSPPTTTPSSTGNISSVGTFKSSNNTTTTGTSRGSNISSNSGFSVASGEDA 60

Query: 61  FPHGQILPSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVI 120
           +P GQILP  NLR +SLAEL+A+T+NFR+E +LGEGGFGKV+KGWLE+K  G++ N  VI
Sbjct: 61  YPDGQILPIPNLRIFSLAELRASTRNFRSENVLGEGGFGKVFKGWLEDKTPGKQSNGTVI 120

Query: 121 AVKKLKSDSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHL 180
           AVKKL ++S QG EEWQ EV FLGR+SHPNLVKLLGYC E  ELLL YE+MQKGSLENHL
Sbjct: 121 AVKKLNAESFQGFEEWQCEVNFLGRVSHPNLVKLLGYCLEGEELLLVYEYMQKGSLENHL 180

Query: 181 FGRGSAVTPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFG 240
           F +GSAV PL W+ RLKIAIGAA+GLAFLH S+KQVIYRDFKASNILLDGSY AK+SDFG
Sbjct: 181 FRKGSAVQPLSWEIRLKIAIGAAKGLAFLHASEKQVIYRDFKASNILLDGSYNAKISDFG 240

Query: 241 LAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDEN 300
           LAKLGPS S+SH+TTRVMGTHGYAAPEYV TGHLYVKSDVYGFGVVL E+LTGL ALD  
Sbjct: 241 LAKLGPSASQSHITTRVMGTHGYAAPEYVATGHLYVKSDVYGFGVVLAEILTGLHALDPT 300

Query: 301 RPTGQEHLTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSM 360
           RPTGQ +LTEWIKP LSERRKL+++MD RLEGKYP +SAF+VAQLAL+C+  E KNRPSM
Sbjct: 301 RPTGQHNLTEWIKPHLSERRKLRSIMDPRLEGKYPFKSAFRVAQLALKCLGPEPKNRPSM 360

Query: 361 KEVVETLEQIETVNEKLIE-TGNRASRLASNRNAH 388
           KEVVE+LE IE  NEK +E    RAS     +  H
Sbjct: 361 KEVVESLELIEAANEKPLERRTTRASPSIRQQQGH 395

BLAST of CSPI07G01730 vs. ExPASy Swiss-Prot
Match: P27450 (Probable serine/threonine-protein kinase CST OS=Arabidopsis thaliana OX=3702 GN=CST PE=1 SV=2)

HSP 1 Score: 409.5 bits (1051), Expect = 4.7e-113
Identity = 207/383 (54.05%), Postives = 283/383 (73.89%), Query Frame = 0

Query: 12  HTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQILPSSNLREYSLA 71
           H+ +TT + S+  ++ +S T ++T S++ + SQFS  S   +   G++L S NL+ Y+  
Sbjct: 19  HSHATTNNHSNG-TEFSSTTGATTNSSVGQQSQFSDISTGIISDSGKLLESPNLKVYNFL 78

Query: 72  ELKAATKNFRAEALLGEGGFGKVYKGWLEEKGL--GRKGNSMVIAVKKLKSDSVQGIEEW 131
           +LK ATKNF+ +++LG+GGFGKVY+GW++   L   R G+ M++A+K+L S+SVQG  EW
Sbjct: 79  DLKTATKNFKPDSMLGQGGFGKVYRGWVDATTLAPSRVGSGMIVAIKRLNSESVQGFAEW 138

Query: 132 QSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAVTPLGWDTRL 191
           +SEV FLG LSH NLVKLLGYC ED ELLL YEFM KGSLE+HLF R     P  WD R+
Sbjct: 139 RSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESHLFRRND---PFPWDLRI 198

Query: 192 KIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTR 251
           KI IGAARGLAFLH+  ++VIYRDFKASNILLD +Y AKLSDFGLAKLGP++ KSH+TTR
Sbjct: 199 KIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLAKLGPADEKSHVTTR 258

Query: 252 VMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEHLTEWIKPFL 311
           +MGT+GYAAPEY+ TGHLYVKSDV+ FGVVL+E++TGL A +  RP GQE L +W++P L
Sbjct: 259 IMGTYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKRPRGQESLVDWLRPEL 318

Query: 312 SERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETLEQIETVNEK 371
           S + ++K +MD  ++G+Y ++ A ++A++ L CIE + KNRP MKEVVE LE I+ +N  
Sbjct: 319 SNKHRVKQIMDKGIKGQYTTKVATEMARITLSCIEPDPKNRPHMKEVVEVLEHIQGLN-- 378

Query: 372 LIETGNRASRLASNRNAHQPLHH 393
           ++   +   +  +N +   P H+
Sbjct: 379 VVPNRSSTKQAVANSSRSSPHHY 395

BLAST of CSPI07G01730 vs. ExPASy Swiss-Prot
Match: Q06548 (Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN=PBL9 PE=1 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 2.9e-107
Identity = 203/369 (55.01%), Postives = 259/369 (70.19%), Query Frame = 0

Query: 46  SAASGDEVFP----HGQILPSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEE 105
           S AS   V P     G+IL S NL+ +S AELK+AT+NFR +++LGEGGFG V+KGW++E
Sbjct: 30  SKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDE 89

Query: 106 KGL--GRKGNSMVIAVKKLKSDSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLL 165
           K L   R G  +VIAVKKL  D  QG +EW +EV +LG+ SH +LVKL+GYC ED   LL
Sbjct: 90  KSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLL 149

Query: 166 TYEFMQKGSLENHLFGRGSAVTPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNI 225
            YEFM +GSLENHLF RG    PL W  RLK+A+GAA+GLAFLH+S+ +VIYRDFK SNI
Sbjct: 150 VYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNI 209

Query: 226 LLDGSYTAKLSDFGLAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVV 285
           LLD  Y AKLSDFGLAK GP   KSH++TRVMGTHGYAAPEY+ TGHL  KSDVY FGVV
Sbjct: 210 LLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVV 269

Query: 286 LIEMLTGLRALDENRPTGQEHLTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLA 345
           L+E+L+G RA+D+NRP+G+ +L EW KP+L  +RK+  V+D RL+ +Y    A +VA L+
Sbjct: 270 LLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLS 329

Query: 346 LQCIEQEQKNRPSMKEVVETLEQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMK 405
           L+C+  E K RP+M EVV  LE I+++N  +    ++  R    R+       +S +  K
Sbjct: 330 LRCLTTEIKLRPNMSEVVSHLEHIQSLNAAIGGNMDKTDRRMRRRS-------DSVVSKK 389

Query: 406 QYGGHANQT 409
              G A QT
Sbjct: 390 VNAGFARQT 391

BLAST of CSPI07G01730 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 389.0 bits (998), Expect = 6.5e-107
Identity = 209/410 (50.98%), Postives = 276/410 (67.32%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60
           MGIC  + +   +P  +  + S  +  +  + SS++S I  N +            G+IL
Sbjct: 1   MGICLSAQIKAVSPGASPKYMSSEANDSLGSKSSSVS-IRTNPR----------TEGEIL 60

Query: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGL--GRKGNSMVIAVKKL 120
            S NL+ ++ AELKAAT+NFR +++LGEGGFG V+KGW++E+ L   + G  +VIAVKKL
Sbjct: 61  QSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKL 120

Query: 121 KSDSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGS 180
             D  QG +EW +EV +LG+ SHPNLVKL+GYC ED   LL YEFM +GSLENHLF RGS
Sbjct: 121 NQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGS 180

Query: 181 AVTPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLG 240
              PL W  RLK+A+GAA+GLAFLH ++  VIYRDFK SNILLD  Y AKLSDFGLAK G
Sbjct: 181 YFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDG 240

Query: 241 PSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQ 300
           P+  KSH++TR+MGT+GYAAPEY+ TGHL  KSDVY +GVVL+E+L+G RA+D+NRP G+
Sbjct: 241 PTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGE 300

Query: 301 EHLTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVE 360
           + L EW +P L+ +RKL  V+D RL+ +Y    A +VA LAL+C+  E K RP+M EVV 
Sbjct: 301 QKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVS 360

Query: 361 TLEQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQT 409
            LE I+T+N    E G R   +   R   +    +  ++ K   G A QT
Sbjct: 361 HLEHIQTLN----EAGGRNIDMVQRRMRRR--SDSVAINQKPNAGFARQT 393

BLAST of CSPI07G01730 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 8.5e-107
Identity = 200/389 (51.41%), Postives = 271/389 (69.67%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEV-FPHGQI 60
           MG CWG+ + + +P             ++ + S   S  + NS  SAAS         +I
Sbjct: 1   MGNCWGAKISSESPC-----------RSASSPSGGTSKYASNSSVSAASVPPTPRSEDEI 60

Query: 61  LPSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLG--RKGNSMVIAVKK 120
           L ++N++ ++  EL+ AT+NFR +++LGEGGFG V+KGW++EK L   + G  MVIAVKK
Sbjct: 61  LEAANVKAFAFNELRTATRNFRPDSVLGEGGFGSVFKGWIDEKTLAPTKPGTGMVIAVKK 120

Query: 121 LKSDSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRG 180
           L  +  QG  EW +EV +LG+LSHP LV+L+GYC ED + LL YEFM +GSLENHLF R 
Sbjct: 121 LNQEGHQGHREWLAEVNYLGQLSHPYLVRLVGYCVEDEQRLLVYEFMPRGSLENHLFRRS 180

Query: 181 SAVTPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKL 240
           +   PL W+ R+KIA+GAA+GLAFLH+   +VIYRDFK SN+LLD +Y AKLSDFGLAK 
Sbjct: 181 THFQPLSWNLRMKIALGAAKGLAFLHSDKVKVIYRDFKTSNVLLDANYDAKLSDFGLAKD 240

Query: 241 GPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTG 300
           GP+  KSH++TRVMGT+GYAAPEY+ TGHL  KSDVY FGVVL+EML+G RALD+NRPTG
Sbjct: 241 GPTGDKSHVSTRVMGTYGYAAPEYLATGHLTTKSDVYSFGVVLLEMLSGRRALDKNRPTG 300

Query: 301 QEHLTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVV 360
           + +L EW +P+L  +R++  ++D RL G+Y    A + A LALQCI  E KNRP+M++VV
Sbjct: 301 EHNLVEWARPYLMSKRRIFRILDARLGGQYSLAKAQKAATLALQCISVEAKNRPNMEQVV 360

Query: 361 ETLEQIETVNEKLIETGNRASRLASNRNA 387
             LEQ++  + K      +  + +S++NA
Sbjct: 361 AVLEQLQ--DSKETGANPQLQKKSSSKNA 376

BLAST of CSPI07G01730 vs. ExPASy TrEMBL
Match: A0A5D3DI79 (Putative serine/threonine-protein kinase Cx32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G003430 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 2.6e-231
Identity = 408/414 (98.55%), Postives = 410/414 (99.03%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60
           MGICWGSPVDNHTPSTTGH SSVISQTTS TTSSTISNISKNSQFSAASGDEVFPHGQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHLSSVISQTTSQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120
           PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180
           DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE+
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360
           L +WIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL
Sbjct: 301 LMDWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIETVNEKLIETG-NRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414
           EQIETVNEKLIETG NRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR
Sbjct: 361 EQIETVNEKLIETGRNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414

BLAST of CSPI07G01730 vs. ExPASy TrEMBL
Match: A0A1S3BHT1 (probable serine/threonine-protein kinase Cx32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103490212 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 2.6e-231
Identity = 408/414 (98.55%), Postives = 410/414 (99.03%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60
           MGICWGSPVDNHTPSTTGH SSVISQTTS TTSSTISNISKNSQFSAASGDEVFPHGQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHLSSVISQTTSQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120
           PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180
           DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE+
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360
           L +WIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL
Sbjct: 301 LMDWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIETVNEKLIETG-NRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414
           EQIETVNEKLIETG NRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR
Sbjct: 361 EQIETVNEKLIETGRNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414

BLAST of CSPI07G01730 vs. ExPASy TrEMBL
Match: A0A0A0K2V0 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G024130 PE=3 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.6e-225
Identity = 398/399 (99.75%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 15  STTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQILPSSNLREYSLAELK 74
           STTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQILPSSNLREYSLAELK
Sbjct: 89  STTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQILPSSNLREYSLAELK 148

Query: 75  AATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKSDSVQGIEEWQSEVG 134
           AATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKSDSVQG+EEWQSEVG
Sbjct: 149 AATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKSDSVQGLEEWQSEVG 208

Query: 135 FLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAVTPLGWDTRLKIAIG 194
           FLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAVTPLGWDTRLKIAIG
Sbjct: 209 FLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAVTPLGWDTRLKIAIG 268

Query: 195 AARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTRVMGTH 254
           AARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTRVMGTH
Sbjct: 269 AARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTRVMGTH 328

Query: 255 GYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEHLTEWIKPFLSERRK 314
           GYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEHLTEWIKPFLSERRK
Sbjct: 329 GYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEHLTEWIKPFLSERRK 388

Query: 315 LKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETLEQIETVNEKLIETG 374
           LKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETLEQIETVNEKLIETG
Sbjct: 389 LKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETLEQIETVNEKLIETG 448

Query: 375 NRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414
           NRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR
Sbjct: 449 NRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 487

BLAST of CSPI07G01730 vs. ExPASy TrEMBL
Match: A0A6J1C6Y9 (probable serine/threonine-protein kinase PIX13 OS=Momordica charantia OX=3673 GN=LOC111007944 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 3.6e-209
Identity = 365/413 (88.38%), Postives = 389/413 (94.19%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60
           MGICWGS V NHTP+TTG+ SSV+SQTTS TTSST+SNIS+NS FSAASGDE +P+GQIL
Sbjct: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60

Query: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120
           PS NL+EYSLAELKAATKNFR EALLGEGGFGKVYKGWLEEKG G++GN MVIAVKKLKS
Sbjct: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120

Query: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDH+ LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE+
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360
           L +WIKP+LSERR+LKNVMDFRLEGKYPSRSAFQVAQLALQCIE EQ+NRPSMKEVVETL
Sbjct: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360

Query: 361 EQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414
           EQIE  NEK  E  NRA+R+ASNRNAHQPLHHNSPLHMK+ G HA++TPPR R
Sbjct: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413

BLAST of CSPI07G01730 vs. ExPASy TrEMBL
Match: A0A6J1GJZ7 (probable serine/threonine-protein kinase PIX13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454971 PE=3 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.2e-205
Identity = 369/415 (88.92%), Postives = 386/415 (93.01%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60
           MGICWGSP DNHTP+TTGH SSV+SQ     T+ST+SNISKNSQFSAASGDE FPHGQ  
Sbjct: 1   MGICWGSPDDNHTPNTTGHLSSVVSQ----ATASTVSNISKNSQFSAASGDEAFPHGQNF 60

Query: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120
            S NLRE+SLAEL+AATKNFRA+ALLGEGGFGKVYKGWLEEKG GRKG SMVIAVKKLKS
Sbjct: 61  LSPNLREHSLAELRAATKNFRADALLGEGGFGKVYKGWLEEKGHGRKGTSMVIAVKKLKS 120

Query: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLG LSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGSLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLKIAIGAARGLAFLH SD QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHASDNQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300
           ESKSHLTTRVMGTHGYAAPEY+TTGHLYVKSDVYGFGVVLIE+LTGLRALDENRPTGQE+
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYITTGHLYVKSDVYGFGVVLIEILTGLRALDENRPTGQEN 300

Query: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360
           L +WIKP+LSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIE+EQKNRP MK+VVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEKEQKNRPPMKDVVETL 360

Query: 361 EQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGG-HANQ-TPPRCR 414
           EQIET NEK IE   RASRLASNRNAHQPLHHNSP+H+KQYGG HA Q TPPR R
Sbjct: 361 EQIET-NEKPIEPRPRASRLASNRNAHQPLHHNSPIHVKQYGGIHAYQTTPPRLR 410

BLAST of CSPI07G01730 vs. NCBI nr
Match: XP_004154222.1 (probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_011658631.1 probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_031744213.1 probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_031744214.1 probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >KAE8645697.1 hypothetical protein Csa_020515 [Cucumis sativus])

HSP 1 Score: 825.5 bits (2131), Expect = 2.1e-235
Identity = 412/413 (99.76%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60
           MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120
           PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180
           DSVQG+EEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGLEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300

Query: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360
           LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL
Sbjct: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414
           EQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR
Sbjct: 361 EQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 413

BLAST of CSPI07G01730 vs. NCBI nr
Match: XP_008447841.1 (PREDICTED: probable serine/threonine-protein kinase Cx32, chloroplastic [Cucumis melo] >TYK23285.1 putative serine/threonine-protein kinase Cx32 [Cucumis melo var. makuwa])

HSP 1 Score: 810.8 bits (2093), Expect = 5.3e-231
Identity = 408/414 (98.55%), Postives = 410/414 (99.03%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60
           MGICWGSPVDNHTPSTTGH SSVISQTTS TTSSTISNISKNSQFSAASGDEVFPHGQIL
Sbjct: 1   MGICWGSPVDNHTPSTTGHLSSVISQTTSQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120
           PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120

Query: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180
           DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE+
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360
           L +WIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL
Sbjct: 301 LMDWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIETVNEKLIETG-NRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414
           EQIETVNEKLIETG NRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR
Sbjct: 361 EQIETVNEKLIETGRNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414

BLAST of CSPI07G01730 vs. NCBI nr
Match: XP_038886859.1 (probable serine/threonine-protein kinase PIX13 [Benincasa hispida] >XP_038886860.1 probable serine/threonine-protein kinase PIX13 [Benincasa hispida] >XP_038886861.1 probable serine/threonine-protein kinase PIX13 [Benincasa hispida])

HSP 1 Score: 785.4 bits (2027), Expect = 2.4e-223
Identity = 393/413 (95.16%), Postives = 402/413 (97.34%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60
           MGICWGSPVDNHTP+TTGH SSV+SQTT+ TTSSTISNISKNSQFSAASGDEVFPHGQIL
Sbjct: 1   MGICWGSPVDNHTPNTTGHLSSVVSQTTNQTTSSTISNISKNSQFSAASGDEVFPHGQIL 60

Query: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120
           PSSNLREYSLAELKAATKNFRAEA+LGEGGFGKVYKGWLEEKGLGRKG SMVIAVKKLKS
Sbjct: 61  PSSNLREYSLAELKAATKNFRAEAMLGEGGFGKVYKGWLEEKGLGRKGYSMVIAVKKLKS 120

Query: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE+
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360
           L +WIKP+LSERRKLKNVMDFRLEGKYPSRSA+QVAQLALQCIEQEQKNRPSMKEVVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAYQVAQLALQCIEQEQKNRPSMKEVVETL 360

Query: 361 EQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414
           EQIET NEK IE  NRASRLASNRNAHQPLHHNSPLHMKQYG HANQTPPR R
Sbjct: 361 EQIETANEKPIEPRNRASRLASNRNAHQPLHHNSPLHMKQYGIHANQTPPRLR 413

BLAST of CSPI07G01730 vs. NCBI nr
Match: XP_022136188.1 (probable serine/threonine-protein kinase PIX13 [Momordica charantia])

HSP 1 Score: 737.3 bits (1902), Expect = 7.4e-209
Identity = 365/413 (88.38%), Postives = 389/413 (94.19%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60
           MGICWGS V NHTP+TTG+ SSV+SQTTS TTSST+SNIS+NS FSAASGDE +P+GQIL
Sbjct: 1   MGICWGSQVQNHTPNTTGNLSSVVSQTTSQTTSSTVSNISRNSLFSAASGDEAYPNGQIL 60

Query: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120
           PS NL+EYSLAELKAATKNFR EALLGEGGFGKVYKGWLEEKG G++GN MVIAVKKLKS
Sbjct: 61  PSPNLKEYSLAELKAATKNFRPEALLGEGGFGKVYKGWLEEKGYGKRGNPMVIAVKKLKS 120

Query: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDH+ LL YEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSMQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHQFLLAYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLK+AIGAARGLAFLHTSD+QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLQWDTRLKVAIGAARGLAFLHTSDEQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQE+
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEN 300

Query: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360
           L +WIKP+LSERR+LKNVMDFRLEGKYPSRSAFQVAQLALQCIE EQ+NRPSMKEVVETL
Sbjct: 301 LLDWIKPYLSERRRLKNVMDFRLEGKYPSRSAFQVAQLALQCIEHEQRNRPSMKEVVETL 360

Query: 361 EQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGGHANQTPPRCR 414
           EQIE  NEK  E  NRA+R+ASNRNAHQPLHHNSPLHMK+ G HA++TPPR R
Sbjct: 361 EQIEATNEKPTEPRNRATRVASNRNAHQPLHHNSPLHMKKNGIHASETPPRLR 413

BLAST of CSPI07G01730 vs. NCBI nr
Match: XP_023554496.1 (probable serine/threonine-protein kinase PIX13 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 727.2 bits (1876), Expect = 7.7e-206
Identity = 371/415 (89.40%), Postives = 387/415 (93.25%), Query Frame = 0

Query: 1   MGICWGSPVDNHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQIL 60
           MG CWGSPVDNHTP+TTGH SSV+SQ     T+ST+SNISKNSQFSAASGDE FPHGQ L
Sbjct: 1   MGTCWGSPVDNHTPNTTGHLSSVVSQ----ATASTVSNISKNSQFSAASGDEAFPHGQNL 60

Query: 61  PSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIAVKKLKS 120
            S NLRE+SLAEL+AATKNFRA+ALLGEGGFGKVYKGWLEEKG GRKG SMVIAVKKLKS
Sbjct: 61  VSPNLREHSLAELRAATKNFRADALLGEGGFGKVYKGWLEEKGHGRKGTSMVIAVKKLKS 120

Query: 121 DSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180
           DS+QGIEEWQSEVGFLG LSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV
Sbjct: 121 DSLQGIEEWQSEVGFLGSLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAV 180

Query: 181 TPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240
           TPL WDTRLKIAIGAARGLAFLH SD QVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS
Sbjct: 181 TPLEWDTRLKIAIGAARGLAFLHASDNQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPS 240

Query: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEH 300
           ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIE+LTGLRALDENRPTGQE+
Sbjct: 241 ESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEILTGLRALDENRPTGQEN 300

Query: 301 LTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETL 360
           L +WIKP+LSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIE+EQKNRP MK+VVETL
Sbjct: 301 LVDWIKPYLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEKEQKNRPPMKDVVETL 360

Query: 361 EQIETVNEKLIETGNRASRLASNRNAHQPLHHNSPLHMKQYGG-HANQ-TPPRCR 414
           EQIET NEK IE   RASRLASNRNAHQPLHHNSP+H+KQYGG HA Q TPPR R
Sbjct: 361 EQIET-NEKPIEPRPRASRLASNRNAHQPLHHNSPIHVKQYGGIHAYQTTPPRLR 410

BLAST of CSPI07G01730 vs. TAIR 10
Match: AT2G17220.1 (Protein kinase superfamily protein )

HSP 1 Score: 540.0 bits (1390), Expect = 1.6e-153
Identity = 279/395 (70.63%), Postives = 321/395 (81.27%), Query Frame = 0

Query: 1   MGICWGSPVDN---HTPSTTGHFSSV----ISQTTSLTTSSTISNISKNSQFSAASGDEV 60
           MG+CWGSP D+    TPS+TG+ SSV     S  T+ T +S  SNIS NS FS ASG++ 
Sbjct: 1   MGLCWGSPSDSPPTTTPSSTGNISSVGTFKSSNNTTTTGTSRGSNISSNSGFSVASGEDA 60

Query: 61  FPHGQILPSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVI 120
           +P GQILP  NLR +SLAEL+A+T+NFR+E +LGEGGFGKV+KGWLE+K  G++ N  VI
Sbjct: 61  YPDGQILPIPNLRIFSLAELRASTRNFRSENVLGEGGFGKVFKGWLEDKTPGKQSNGTVI 120

Query: 121 AVKKLKSDSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHL 180
           AVKKL ++S QG EEWQ EV FLGR+SHPNLVKLLGYC E  ELLL YE+MQKGSLENHL
Sbjct: 121 AVKKLNAESFQGFEEWQCEVNFLGRVSHPNLVKLLGYCLEGEELLLVYEYMQKGSLENHL 180

Query: 181 FGRGSAVTPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFG 240
           F +GSAV PL W+ RLKIAIGAA+GLAFLH S+KQVIYRDFKASNILLDGSY AK+SDFG
Sbjct: 181 FRKGSAVQPLSWEIRLKIAIGAAKGLAFLHASEKQVIYRDFKASNILLDGSYNAKISDFG 240

Query: 241 LAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDEN 300
           LAKLGPS S+SH+TTRVMGTHGYAAPEYV TGHLYVKSDVYGFGVVL E+LTGL ALD  
Sbjct: 241 LAKLGPSASQSHITTRVMGTHGYAAPEYVATGHLYVKSDVYGFGVVLAEILTGLHALDPT 300

Query: 301 RPTGQEHLTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSM 360
           RPTGQ +LTEWIKP LSERRKL+++MD RLEGKYP +SAF+VAQLAL+C+  E KNRPSM
Sbjct: 301 RPTGQHNLTEWIKPHLSERRKLRSIMDPRLEGKYPFKSAFRVAQLALKCLGPEPKNRPSM 360

Query: 361 KEVVETLEQIETVNEKLIE-TGNRASRLASNRNAH 388
           KEVVE+LE IE  NEK +E    RAS     +  H
Sbjct: 361 KEVVESLELIEAANEKPLERRTTRASPSIRQQQGH 395

BLAST of CSPI07G01730 vs. TAIR 10
Match: AT2G17220.2 (Protein kinase superfamily protein )

HSP 1 Score: 538.1 bits (1385), Expect = 6.2e-153
Identity = 278/394 (70.56%), Postives = 320/394 (81.22%), Query Frame = 0

Query: 1   MGICWGSPVDN---HTPSTTGHFSS---VISQTTSLTTSSTISNISKNSQFSAASGDEVF 60
           MG+CWGSP D+    TPS+TG+ SS     S  T+ T +S  SNIS NS FS ASG++ +
Sbjct: 1   MGLCWGSPSDSPPTTTPSSTGNISSGTFKSSNNTTTTGTSRGSNISSNSGFSVASGEDAY 60

Query: 61  PHGQILPSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGLGRKGNSMVIA 120
           P GQILP  NLR +SLAEL+A+T+NFR+E +LGEGGFGKV+KGWLE+K  G++ N  VIA
Sbjct: 61  PDGQILPIPNLRIFSLAELRASTRNFRSENVLGEGGFGKVFKGWLEDKTPGKQSNGTVIA 120

Query: 121 VKKLKSDSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLF 180
           VKKL ++S QG EEWQ EV FLGR+SHPNLVKLLGYC E  ELLL YE+MQKGSLENHLF
Sbjct: 121 VKKLNAESFQGFEEWQCEVNFLGRVSHPNLVKLLGYCLEGEELLLVYEYMQKGSLENHLF 180

Query: 181 GRGSAVTPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGL 240
            +GSAV PL W+ RLKIAIGAA+GLAFLH S+KQVIYRDFKASNILLDGSY AK+SDFGL
Sbjct: 181 RKGSAVQPLSWEIRLKIAIGAAKGLAFLHASEKQVIYRDFKASNILLDGSYNAKISDFGL 240

Query: 241 AKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENR 300
           AKLGPS S+SH+TTRVMGTHGYAAPEYV TGHLYVKSDVYGFGVVL E+LTGL ALD  R
Sbjct: 241 AKLGPSASQSHITTRVMGTHGYAAPEYVATGHLYVKSDVYGFGVVLAEILTGLHALDPTR 300

Query: 301 PTGQEHLTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMK 360
           PTGQ +LTEWIKP LSERRKL+++MD RLEGKYP +SAF+VAQLAL+C+  E KNRPSMK
Sbjct: 301 PTGQHNLTEWIKPHLSERRKLRSIMDPRLEGKYPFKSAFRVAQLALKCLGPEPKNRPSMK 360

Query: 361 EVVETLEQIETVNEKLIE-TGNRASRLASNRNAH 388
           EVVE+LE IE  NEK +E    RAS     +  H
Sbjct: 361 EVVESLELIEAANEKPLERRTTRASPSIRQQQGH 394

BLAST of CSPI07G01730 vs. TAIR 10
Match: AT4G35600.1 (Protein kinase superfamily protein )

HSP 1 Score: 409.5 bits (1051), Expect = 3.3e-114
Identity = 207/383 (54.05%), Postives = 283/383 (73.89%), Query Frame = 0

Query: 12  HTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQILPSSNLREYSLA 71
           H+ +TT + S+  ++ +S T ++T S++ + SQFS  S   +   G++L S NL+ Y+  
Sbjct: 19  HSHATTNNHSNG-TEFSSTTGATTNSSVGQQSQFSDISTGIISDSGKLLESPNLKVYNFL 78

Query: 72  ELKAATKNFRAEALLGEGGFGKVYKGWLEEKGL--GRKGNSMVIAVKKLKSDSVQGIEEW 131
           +LK ATKNF+ +++LG+GGFGKVY+GW++   L   R G+ M++A+K+L S+SVQG  EW
Sbjct: 79  DLKTATKNFKPDSMLGQGGFGKVYRGWVDATTLAPSRVGSGMIVAIKRLNSESVQGFAEW 138

Query: 132 QSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAVTPLGWDTRL 191
           +SEV FLG LSH NLVKLLGYC ED ELLL YEFM KGSLE+HLF R     P  WD R+
Sbjct: 139 RSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESHLFRRND---PFPWDLRI 198

Query: 192 KIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTTR 251
           KI IGAARGLAFLH+  ++VIYRDFKASNILLD +Y AKLSDFGLAKLGP++ KSH+TTR
Sbjct: 199 KIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLAKLGPADEKSHVTTR 258

Query: 252 VMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEHLTEWIKPFL 311
           +MGT+GYAAPEY+ TGHLYVKSDV+ FGVVL+E++TGL A +  RP GQE L +W++P L
Sbjct: 259 IMGTYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKRPRGQESLVDWLRPEL 318

Query: 312 SERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETLEQIETVNEK 371
           S + ++K +MD  ++G+Y ++ A ++A++ L CIE + KNRP MKEVVE LE I+ +N  
Sbjct: 319 SNKHRVKQIMDKGIKGQYTTKVATEMARITLSCIEPDPKNRPHMKEVVEVLEHIQGLN-- 378

Query: 372 LIETGNRASRLASNRNAHQPLHH 393
           ++   +   +  +N +   P H+
Sbjct: 379 VVPNRSSTKQAVANSSRSSPHHY 395

BLAST of CSPI07G01730 vs. TAIR 10
Match: AT4G35600.2 (Protein kinase superfamily protein )

HSP 1 Score: 409.1 bits (1050), Expect = 4.3e-114
Identity = 206/384 (53.65%), Postives = 281/384 (73.18%), Query Frame = 0

Query: 11  NHTPSTTGHFSSVISQTTSLTTSSTISNISKNSQFSAASGDEVFPHGQILPSSNLREYSL 70
           +H  +T  H +   ++ +S T ++T S++ + SQFS  S   +   G++L S NL+ Y+ 
Sbjct: 20  SHAATTNNHSNG--TEFSSTTGATTNSSVGQQSQFSDISTGIISDSGKLLESPNLKVYNF 79

Query: 71  AELKAATKNFRAEALLGEGGFGKVYKGWLEEKGL--GRKGNSMVIAVKKLKSDSVQGIEE 130
            +LK ATKNF+ +++LG+GGFGKVY+GW++   L   R G+ M++A+K+L S+SVQG  E
Sbjct: 80  LDLKTATKNFKPDSMLGQGGFGKVYRGWVDATTLAPSRVGSGMIVAIKRLNSESVQGFAE 139

Query: 131 WQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENHLFGRGSAVTPLGWDTR 190
           W+SEV FLG LSH NLVKLLGYC ED ELLL YEFM KGSLE+HLF R     P  WD R
Sbjct: 140 WRSEVNFLGMLSHRNLVKLLGYCREDKELLLVYEFMPKGSLESHLFRRND---PFPWDLR 199

Query: 191 LKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDFGLAKLGPSESKSHLTT 250
           +KI IGAARGLAFLH+  ++VIYRDFKASNILLD +Y AKLSDFGLAKLGP++ KSH+TT
Sbjct: 200 IKIVIGAARGLAFLHSLQREVIYRDFKASNILLDSNYDAKLSDFGLAKLGPADEKSHVTT 259

Query: 251 RVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDENRPTGQEHLTEWIKPF 310
           R+MGT+GYAAPEY+ TGHLYVKSDV+ FGVVL+E++TGL A +  RP GQE L +W++P 
Sbjct: 260 RIMGTYGYAAPEYMATGHLYVKSDVFAFGVVLLEIMTGLTAHNTKRPRGQESLVDWLRPE 319

Query: 311 LSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPSMKEVVETLEQIETVNE 370
           LS + ++K +MD  ++G+Y ++ A ++A++ L CIE + KNRP MKEVVE LE I+ +N 
Sbjct: 320 LSNKHRVKQIMDKGIKGQYTTKVATEMARITLSCIEPDPKNRPHMKEVVEVLEHIQGLN- 379

Query: 371 KLIETGNRASRLASNRNAHQPLHH 393
            ++   +   +  +N +   P H+
Sbjct: 380 -VVPNRSSTKQAVANSSRSSPHHY 396

BLAST of CSPI07G01730 vs. TAIR 10
Match: AT1G76360.1 (Protein kinase superfamily protein )

HSP 1 Score: 399.1 bits (1024), Expect = 4.5e-111
Identity = 195/326 (59.82%), Postives = 251/326 (76.99%), Query Frame = 0

Query: 55  PHGQILPSSNLREYSLAELKAATKNFRAEALLGEGGFGKVYKGWLEEKGL--GRKGNSMV 114
           P G+I+ + NL+ ++L ELK ATKNFR E+++GEGGFG+V+KGW++EK L   R G  + 
Sbjct: 139 PSGKIV-TPNLKMFTLVELKTATKNFRPESVIGEGGFGQVFKGWVDEKTLAPSRAGVGIP 198

Query: 115 IAVKKLKSDSVQGIEEWQSEVGFLGRLSHPNLVKLLGYCWEDHELLLTYEFMQKGSLENH 174
           +AVKK   DS QG+ EWQ EV FLG+  HPNLVKLLGYCWE+++ LL YE++ KGSLENH
Sbjct: 199 VAVKKSNPDSEQGLHEWQCEVRFLGKFHHPNLVKLLGYCWEENQFLLVYEYLPKGSLENH 258

Query: 175 LFGRGSAVTPLGWDTRLKIAIGAARGLAFLHTSDKQVIYRDFKASNILLDGSYTAKLSDF 234
           LF +G+   P  WDTRLKIAI AA+GL FLH S+K VIYRDFKASNILLD ++ AKLSDF
Sbjct: 259 LFSKGAEALP--WDTRLKIAIEAAQGLTFLHNSEKSVIYRDFKASNILLDSNFHAKLSDF 318

Query: 235 GLAKLGPSESKSHLTTRVMGTHGYAAPEYVTTGHLYVKSDVYGFGVVLIEMLTGLRALDE 294
           GLAK GP    SH+TTRVMGT GYAAPEY+ TGHLYV+SDVYGFGVVL+E+LTGLRALD 
Sbjct: 319 GLAKNGPINGFSHVTTRVMGTQGYAAPEYMATGHLYVRSDVYGFGVVLLELLTGLRALDP 378

Query: 295 NRPTGQEHLTEWIKPFLSERRKLKNVMDFRLEGKYPSRSAFQVAQLALQCIEQEQKNRPS 354
           NRP+ Q++L EW KP L++++K++ +MD RLE KYP  +  + A+L L+C+E + KNRP 
Sbjct: 379 NRPSAQQNLVEWAKPGLNQKKKVQKMMDPRLEQKYPLLAVTKTAELILRCLEADPKNRPP 438

Query: 355 MKEVVETLEQIETVNEKLIETGNRAS 379
           M +V+  LE + T+ ++  E   + S
Sbjct: 439 MDDVLRELEVVRTIRDQPQEERRKRS 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SII62.3e-15270.63Probable serine/threonine-protein kinase PIX13 OS=Arabidopsis thaliana OX=3702 G... [more]
P274504.7e-11354.05Probable serine/threonine-protein kinase CST OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q065482.9e-10755.01Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN... [more]
P465736.5e-10750.98Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q65XV88.5e-10751.41Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A5D3DI792.6e-23198.55Putative serine/threonine-protein kinase Cx32 OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3BHT12.6e-23198.55probable serine/threonine-protein kinase Cx32, chloroplastic OS=Cucumis melo OX=... [more]
A0A0A0K2V01.6e-22599.75Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G024... [more]
A0A6J1C6Y93.6e-20988.38probable serine/threonine-protein kinase PIX13 OS=Momordica charantia OX=3673 GN... [more]
A0A6J1GJZ73.2e-20588.92probable serine/threonine-protein kinase PIX13 isoform X1 OS=Cucurbita moschata ... [more]
Match NameE-valueIdentityDescription
XP_004154222.12.1e-23599.76probable serine/threonine-protein kinase PIX13 [Cucumis sativus] >XP_011658631.1... [more]
XP_008447841.15.3e-23198.55PREDICTED: probable serine/threonine-protein kinase Cx32, chloroplastic [Cucumis... [more]
XP_038886859.12.4e-22395.16probable serine/threonine-protein kinase PIX13 [Benincasa hispida] >XP_038886860... [more]
XP_022136188.17.4e-20988.38probable serine/threonine-protein kinase PIX13 [Momordica charantia][more]
XP_023554496.17.7e-20689.40probable serine/threonine-protein kinase PIX13 isoform X1 [Cucurbita pepo subsp.... [more]
Match NameE-valueIdentityDescription
AT2G17220.11.6e-15370.63Protein kinase superfamily protein [more]
AT2G17220.26.2e-15370.56Protein kinase superfamily protein [more]
AT4G35600.13.3e-11454.05Protein kinase superfamily protein [more]
AT4G35600.24.3e-11453.65Protein kinase superfamily protein [more]
AT1G76360.14.5e-11159.82Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 353..373
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 64..163
e-value: 7.2E-32
score: 111.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 164..386
e-value: 2.3E-60
score: 205.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 386..413
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 11..391
NoneNo IPR availablePANTHERPTHR45621:SF169BNAA09G08980D PROTEINcoord: 11..391
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 86..363
e-value: 1.49285E-93
score: 280.698
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 83..360
e-value: 7.1E-46
score: 156.6
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 209..221
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 86..117
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 80..363
score: 38.717987
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 60..363

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G01730.1CSPI07G01730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity