CSPI06G32350 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G32350
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionendochitinase-like
LocationChr6: 27260275 .. 27261780 (-)
RNA-Seq ExpressionCSPI06G32350
SyntenyCSPI06G32350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGATGTTAATCACATCAAGGAAAAATATGAAGACATATTCACTTGTAATCTTATGCTTTGCCTTTCTTTTGGTAGCTGCCGCTGCCGAGCAATGTGGACGGCAGGCCAATGGGGCTCTTTGCCCCAATAACCTCTGCTGCAGCCAGTTCGGGTTCTGCGGTGATACCGATGACTATTGTAAAAATGGTTGTCAAAGCCAGTGTCGCGGCTCTAGTACTCCTACGCCATCTGGCGGTAGCGGCGTCGGAAGTATCATAAGCGAAAGTCTTTACAATCAAATGCTCAAATATAGTAGGGATTCTCGATGTCCTAGTAATGGATTCTATACCTATAGTGCTTTCATTACTGCTGCTCGATCCTTCCCGGCCTTCGGTAACACGGGAAGTGTAGAGACTCGTAAGAGGGAAGTTGCAGCTTTTTTCGGTCAAACTTCTCACGAGACTACAGGTTTGTTTCAAAAACTATCGACCTCTTTTATTTATTATTGTTTTGTAAAACTATCTTTAATTTTTTCTTAGTACTGATCTCTAGACTTAAAATTAACTCGCATGTGACGATGTAGGAGGATGGCCCACTGCACCAGATGGCCCATACGCATGGGGATACTGTTTCATTCGAGAGAGAAATCAACAAACATATTGCACACCTAGTCAGCAATGGCCGTGTGCTTCTGGTCAACAATATTACGGTCGTGGACCAATCCAACTAACCCAGTGAGTCTTTTTTTTTTTTTTAACATCATTTTCATTTTTTTTTTCAATTTTAGAGTTTGTTCAATATTAGTTTTTGTACTTAGAATTAGTTTCTCAGTGATGTTAACTAGCATCGTATTAAAATGATTGGACTTTTTTTAGCAACTACAACTACGGACCAGCAGGGAATGCAATAGGAGCGCCGTTGCTAGCCAGCCCTGATTTGGTAGCCACAGATGCAGTTGTATCCTTTAAGACAGCCCTATGGTTTTGGATGACAGCACAAGGAAATAAACCATCTTGTCATAATGTTATTATCGGCAATTGGCAACCTTCGAGCGCTGACAATGCTGCAGGAAGATCCACTGGCTATGGTGTCATCACCAACATCATTAATGGTGGACTCGAGTGTGGACATGGTCCTGATGATAGAGTTAAAGACAGAATTGGATTTTACAAACGCTACTGTGACATGCTGGGTATTGGTTATGGCAACAACTTAGATTGCTACAATCAAAGGTCTTTCTAGATATTTATTGATCTTACAACATATATGGAATAAAATTAAAAGTGTCTTCTCCTTGTAAGATGCAGTAAGACTGTGACTTGGTGGTATGTGTAATGTCAAAACTTATGTAATAATAAAGATTAGAGAGAGGGAAATTCACTTTCCCCATAAATTATAATAATCAATACAAAATGATTTTGTGCCTCTTCTTTCATTTCCACACAATTAAACTTTATGTTAAAATTATGTATACTCACATATCAACTACATCAAATAAAAATCCACTAAACACCATCACCCTATAC

mRNA sequence

TCGATGTTAATCACATCAAGGAAAAATATGAAGACATATTCACTTGTAATCTTATGCTTTGCCTTTCTTTTGGTAGCTGCCGCTGCCGAGCAATGTGGACGGCAGGCCAATGGGGCTCTTTGCCCCAATAACCTCTGCTGCAGCCAGTTCGGGTTCTGCGGTGATACCGATGACTATTGTAAAAATGGTTGTCAAAGCCAGTGTCGCGGCTCTAGTACTCCTACGCCATCTGGCGGTAGCGGCGTCGGAAGTATCATAAGCGAAAGTCTTTACAATCAAATGCTCAAATATAGTAGGGATTCTCGATGTCCTAGTAATGGATTCTATACCTATAGTGCTTTCATTACTGCTGCTCGATCCTTCCCGGCCTTCGGTAACACGGGAAGTGTAGAGACTCGTAAGAGGGAAGTTGCAGCTTTTTTCGGTCAAACTTCTCACGAGACTACAGGAGGATGGCCCACTGCACCAGATGGCCCATACGCATGGGGATACTGTTTCATTCGAGAGAGAAATCAACAAACATATTGCACACCTAGTCAGCAATGGCCGTGTGCTTCTGGTCAACAATATTACGGTCGTGGACCAATCCAACTAACCCACAACTACAACTACGGACCAGCAGGGAATGCAATAGGAGCGCCGTTGCTAGCCAGCCCTGATTTGGTAGCCACAGATGCAGTTGTATCCTTTAAGACAGCCCTATGGTTTTGGATGACAGCACAAGGAAATAAACCATCTTGTCATAATGTTATTATCGGCAATTGGCAACCTTCGAGCGCTGACAATGCTGCAGGAAGATCCACTGGCTATGGTGTCATCACCAACATCATTAATGGTGGACTCGAGTGTGGACATGGTCCTGATGATAGAGTTAAAGACAGAATTGGATTTTACAAACGCTACTGTGACATGCTGGGTATTGGTTATGGCAACAACTTAGATTGCTACAATCAAAGGTCTTTCTAGATATTTATTGATCTTACAACATATATGGAATAAAATTAAAAGTGTCTTCTCCTTGTAAGATGCAGTAAGACTGTGACTTGGTGGTATGTGTAATGTCAAAACTTATGTAATAATAAAGATTAGAGAGAGGGAAATTCACTTTCCCCATAAATTATAATAATCAATACAAAATGATTTTGTGCCTCTTCTTTCATTTCCACACAATTAAACTTTATGTTAAAATTATGTATACTCACATATCAACTACATCAAATAAAAATCCACTAAACACCATCACCCTATAC

Coding sequence (CDS)

ATGTTAATCACATCAAGGAAAAATATGAAGACATATTCACTTGTAATCTTATGCTTTGCCTTTCTTTTGGTAGCTGCCGCTGCCGAGCAATGTGGACGGCAGGCCAATGGGGCTCTTTGCCCCAATAACCTCTGCTGCAGCCAGTTCGGGTTCTGCGGTGATACCGATGACTATTGTAAAAATGGTTGTCAAAGCCAGTGTCGCGGCTCTAGTACTCCTACGCCATCTGGCGGTAGCGGCGTCGGAAGTATCATAAGCGAAAGTCTTTACAATCAAATGCTCAAATATAGTAGGGATTCTCGATGTCCTAGTAATGGATTCTATACCTATAGTGCTTTCATTACTGCTGCTCGATCCTTCCCGGCCTTCGGTAACACGGGAAGTGTAGAGACTCGTAAGAGGGAAGTTGCAGCTTTTTTCGGTCAAACTTCTCACGAGACTACAGGAGGATGGCCCACTGCACCAGATGGCCCATACGCATGGGGATACTGTTTCATTCGAGAGAGAAATCAACAAACATATTGCACACCTAGTCAGCAATGGCCGTGTGCTTCTGGTCAACAATATTACGGTCGTGGACCAATCCAACTAACCCACAACTACAACTACGGACCAGCAGGGAATGCAATAGGAGCGCCGTTGCTAGCCAGCCCTGATTTGGTAGCCACAGATGCAGTTGTATCCTTTAAGACAGCCCTATGGTTTTGGATGACAGCACAAGGAAATAAACCATCTTGTCATAATGTTATTATCGGCAATTGGCAACCTTCGAGCGCTGACAATGCTGCAGGAAGATCCACTGGCTATGGTGTCATCACCAACATCATTAATGGTGGACTCGAGTGTGGACATGGTCCTGATGATAGAGTTAAAGACAGAATTGGATTTTACAAACGCTACTGTGACATGCTGGGTATTGGTTATGGCAACAACTTAGATTGCTACAATCAAAGGTCTTTCTAG

Protein sequence

MLITSRKNMKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYGNNLDCYNQRSF*
Homology
BLAST of CSPI06G32350 vs. ExPASy Swiss-Prot
Match: Q9FRV1 (Basic endochitinase A OS=Secale cereale OX=4550 GN=rsca PE=1 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.2e-127
Identity = 210/310 (67.74%), Postives = 236/310 (76.13%), Query Frame = 0

Query: 20  AFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRG--------SS 79
           A  +  A AEQCG QA GA CPN LCCS+FG+CG T DYC +GCQSQC G        + 
Sbjct: 11  AMAVTMAVAEQCGSQAGGATCPNCLCCSRFGWCGSTSDYCGDGCQSQCAGCGGGGTPVTP 70

Query: 80  TPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGSVET 139
           TPTPSGG GV SI+S +L+++ML +  D  C + GFYTY AF+ AA +FP FG TGS +T
Sbjct: 71  TPTPSGGGGVSSIVSRALFDRMLLHRNDGACQAKGFYTYDAFVAAAGAFPGFGTTGSTDT 130

Query: 140 RKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERN-QQTYCTPSQQWPCASGQQYY 199
           RKREVAAF  QTSHETTGGW TAPDG +AWGYCF +ER     YCTPS QWPCA G+ YY
Sbjct: 131 RKREVAAFLAQTSHETTGGWATAPDGAFAWGYCFKQERGATSNYCTPSAQWPCAPGKSYY 190

Query: 200 GRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHNVI 259
           GRGPIQL+HNYNYGPAG AIG  LL +PDLVATD  VSFKTA+WFWMTAQ  KPS H VI
Sbjct: 191 GRGPIQLSHNYNYGPAGRAIGVDLLRNPDLVATDPTVSFKTAMWFWMTAQAPKPSSHAVI 250

Query: 260 IGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYGN 319
            G W PS  D AAGR  G+GVITNI+NGG+ECGHG D RV DRIGFYKRYCD+L +GYGN
Sbjct: 251 TGQWSPSGTDRAAGRVPGFGVITNIVNGGIECGHGQDSRVADRIGFYKRYCDILRVGYGN 310

Query: 320 NLDCYNQRSF 321
           NLDCYNQR F
Sbjct: 311 NLDCYNQRPF 320

BLAST of CSPI06G32350 vs. ExPASy Swiss-Prot
Match: Q7DNA1 (Chitinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht2 PE=1 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 2.6e-127
Identity = 214/319 (67.08%), Postives = 248/319 (77.74%), Query Frame = 0

Query: 7   KNMKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQ 66
           K     +L +L  A L  AA AEQCG QA GA CPN LCCS++G+CG T D+C +GCQSQ
Sbjct: 12  KKAALVALAVLA-AALATAARAEQCGAQAGGARCPNCLCCSRWGWCGTTSDFCGDGCQSQ 71

Query: 67  CRG---SSTPT-PSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPA 126
           C G   + TPT PS   GVGSI+   L+ ++L +  D  CP+ GFYTY AF+ AA +FPA
Sbjct: 72  CSGCGPTPTPTPPSPSDGVGSIVPRDLFERLLLHRNDGACPARGFYTYEAFLAAAAAFPA 131

Query: 127 FGNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQT-YCTPSQQW 186
           FG TG+ ETRKREVAAF GQTSHETTGGWPTAPDGP++WGYCF +E+N  + YC PS +W
Sbjct: 132 FGGTGNTETRKREVAAFLGQTSHETTGGWPTAPDGPFSWGYCFKQEQNPPSDYCQPSPEW 191

Query: 187 PCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQG 246
           PCA G++YYGRGPIQL+ N+NYGPAG AIG  LL++PDLVATDA VSFKTALWFWMT QG
Sbjct: 192 PCAPGRKYYGRGPIQLSFNFNYGPAGRAIGVDLLSNPDLVATDATVSFKTALWFWMTPQG 251

Query: 247 NKPSCHNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYC 306
           NKPS H+VI G W PS AD AAGR+ GYGVITNI+NGGLECGHGPDDRV +RIGFY+RYC
Sbjct: 252 NKPSSHDVITGRWAPSPADAAAGRAPGYGVITNIVNGGLECGHGPDDRVANRIGFYQRYC 311

Query: 307 DMLGIGYGNNLDCYNQRSF 321
              GIG G NLDCYNQR F
Sbjct: 312 GAFGIGTGGNLDCYNQRPF 329

BLAST of CSPI06G32350 vs. ExPASy Swiss-Prot
Match: P93680 (Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2)

HSP 1 Score: 454.1 bits (1167), Expect = 1.3e-126
Identity = 209/308 (67.86%), Postives = 241/308 (78.25%), Query Frame = 0

Query: 14  LVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRGSSTP 73
           L++L    L   A AEQCGRQA GALCP  LCCSQFG+CG T DYC   CQSQC G    
Sbjct: 11  LLLLLVGLLAGEAFAEQCGRQAGGALCPGGLCCSQFGWCGSTSDYCGPTCQSQCGG---V 70

Query: 74  TPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGSVETRK 133
           TPS G GV S+IS+S++NQMLK+  D+ C + GFYTY+AFI AA SF  F + G   TRK
Sbjct: 71  TPSPGGGVASLISQSVFNQMLKHRNDAACQAKGFYTYNAFIAAANSFNGFASVGDTATRK 130

Query: 134 REVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRER-NQQTYCTPSQQWPCASGQQYYGR 193
           RE+AAF  QTSHETTGGW TAPDGPYAWGYCF++E+ N   YC P+ QWPCA G++YYGR
Sbjct: 131 REIAAFLAQTSHETTGGWATAPDGPYAWGYCFLKEQGNPPDYCVPTAQWPCAPGKKYYGR 190

Query: 194 GPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHNVIIG 253
           GPIQ+++NYNYGPAG AIG  L+ +PD VATD V+SFKTALWFWMT Q  KPSCHNVI G
Sbjct: 191 GPIQISYNYNYGPAGRAIGYDLINNPDAVATDPVISFKTALWFWMTPQSPKPSCHNVITG 250

Query: 254 NWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYGNNL 313
            W PS+AD AAGR  GYGVITNIINGG+ECG G +D+V DRIGFYKRYCD+LG+ YG+NL
Sbjct: 251 RWTPSAADRAAGRLPGYGVITNIINGGIECGKGFNDKVADRIGFYKRYCDLLGVSYGSNL 310

Query: 314 DCYNQRSF 321
           DCYNQRSF
Sbjct: 311 DCYNQRSF 315

BLAST of CSPI06G32350 vs. ExPASy Swiss-Prot
Match: Q42993 (Chitinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht1 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.7e-126
Identity = 207/321 (64.49%), Postives = 243/321 (75.70%), Query Frame = 0

Query: 9   MKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 68
           M+  ++V++  AF +VA   EQCG QA GALCPN LCCSQ+G+CG T  YC +GCQSQC 
Sbjct: 1   MRALAVVVVATAFAVVAVRGEQCGSQAGGALCPNCLCCSQYGWCGSTSAYCGSGCQSQCS 60

Query: 69  GS------STPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPA 128
           GS      + P+  GGSGV SI+S SL++QML +  D+ CP+  FYTY AF+ AA +FP+
Sbjct: 61  GSCGGGGPTPPSGGGGSGVASIVSRSLFDQMLLHRNDAACPAKNFYTYDAFVAAANAFPS 120

Query: 129 FGNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQT---YCTPSQ 188
           F  TG   TRKREVAAF  QTSHETTGGW TAPDGPY+WGYCF  E N      YC  S 
Sbjct: 121 FATTGDAATRKREVAAFLAQTSHETTGGWATAPDGPYSWGYCFKEENNGNVGSDYCVQSS 180

Query: 189 QWPCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTA 248
           QWPCA+G++YYGRGPIQ+++NYNYGPAG AIG+ LL++PDLVA+DA VSFKTA WFWMT 
Sbjct: 181 QWPCAAGKKYYGRGPIQISYNYNYGPAGQAIGSNLLSNPDLVASDATVSFKTAFWFWMTP 240

Query: 249 QGNKPSCHNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKR 308
           Q  KPSCH V+ G W P+  D AAGR  GYGV+TNIINGG+ECGHG D RV DRIGFYKR
Sbjct: 241 QSPKPSCHAVMTGQWTPNGNDQAAGRVPGYGVVTNIINGGVECGHGADSRVADRIGFYKR 300

Query: 309 YCDMLGIGYGNNLDCYNQRSF 321
           YCDMLG+ YG NLDCYNQR F
Sbjct: 301 YCDMLGVSYGANLDCYNQRPF 321

BLAST of CSPI06G32350 vs. ExPASy Swiss-Prot
Match: P08252 (Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2)

HSP 1 Score: 453.8 bits (1166), Expect = 1.7e-126
Identity = 210/316 (66.46%), Postives = 249/316 (78.80%), Query Frame = 0

Query: 10  KTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNG-CQSQCR 69
           K  +L  L F+ LL++A+AEQCG QA GA CP+ LCCS+FG+CG+T+DYC  G CQSQC 
Sbjct: 5   KFTALSSLLFSLLLLSASAEQCGSQAGGARCPSGLCCSKFGWCGNTNDYCGPGNCQSQCP 64

Query: 70  GSST---PTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGN 129
           G  T   PTP GG  +GSIIS S+++QMLK+  D+ C   GFY+Y+AFI AARSFP FG 
Sbjct: 65  GGPTPTPPTPPGGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGT 124

Query: 130 TGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQ-TYCTPSQQWPCA 189
           +G    RKRE+AAFF QTSHETTGGW TAPDGPYAWGYC++RE+     YCTPS QWPCA
Sbjct: 125 SGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCA 184

Query: 190 SGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKP 249
            G++Y+GRGPIQ++HNYNYGP G AIG  LL +PDLVATD V+SFK+ALWFWMT Q  KP
Sbjct: 185 PGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKP 244

Query: 250 SCHNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDML 309
           SCH+VIIG WQPS+ D AA R  G+GVITNIINGGLECG G D RV+DRIGFY+RYC +L
Sbjct: 245 SCHDVIIGRWQPSAGDRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSIL 304

Query: 310 GIGYGNNLDCYNQRSF 321
           G+  G+NLDC NQRSF
Sbjct: 305 GVSPGDNLDCGNQRSF 320

BLAST of CSPI06G32350 vs. ExPASy TrEMBL
Match: A0A0A0KJK4 (Chitin-binding type-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507520 PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 3.2e-189
Identity = 313/320 (97.81%), Postives = 314/320 (98.12%), Query Frame = 0

Query: 1   MLITSRKNMKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCK 60
           MLITSRKNMKTYSLVILCFAFLLV  AAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCK
Sbjct: 1   MLITSRKNMKTYSLVILCFAFLLVVVAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCK 60

Query: 61  NGCQSQCRGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSF 120
           NGCQSQCRGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTY+AFITAAR F
Sbjct: 61  NGCQSQCRGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYNAFITAARFF 120

Query: 121 PAFGNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQ 180
           PAFGNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQ YCTPSQQ
Sbjct: 121 PAFGNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQAYCTPSQQ 180

Query: 181 WPCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQ 240
           WPCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQ
Sbjct: 181 WPCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQ 240

Query: 241 GNKPSCHNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRY 300
           GNKPSCHNVI GNWQPSSADNAAGRS GYGVITNIINGGLECGHGPDDRVKDRIGFYKRY
Sbjct: 241 GNKPSCHNVITGNWQPSSADNAAGRSPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRY 300

Query: 301 CDMLGIGYGNNLDCYNQRSF 321
           CDMLGIGYGNNLDCYNQRSF
Sbjct: 301 CDMLGIGYGNNLDCYNQRSF 320

BLAST of CSPI06G32350 vs. ExPASy TrEMBL
Match: A0A5A7SHJ5 (Family 19 chitinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00730 PE=4 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.4e-175
Identity = 289/312 (92.63%), Postives = 293/312 (93.91%), Query Frame = 0

Query: 9   MKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 68
           MKTYSLVIL FAFLL AA+AEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR
Sbjct: 1   MKTYSLVILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 60

Query: 69  GSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGS 128
           GSSTP PSGGSGVGSIISESLYNQMLKYSRD RCPSNGFYTY+AFITAARSFP FG TG 
Sbjct: 61  GSSTPPPSGGSGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTTGD 120

Query: 129 VETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCASGQQ 188
             TRKRE+AAFFGQTSHETTGGW TAPDGPYAWGYCFIRERNQQTYCTPSQQWPCA GQQ
Sbjct: 121 ATTRKREIAAFFGQTSHETTGGWSTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCAPGQQ 180

Query: 189 YYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHN 248
           YYGRGPIQLTHNYNYGPAG AIGAPLL +PD VATD V SFKTALWFWMTAQGNKPSCHN
Sbjct: 181 YYGRGPIQLTHNYNYGPAGKAIGAPLLTNPDTVATDPVTSFKTALWFWMTAQGNKPSCHN 240

Query: 249 VIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGY 308
           VI GNWQPSSADNAAGR  GYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGY
Sbjct: 241 VITGNWQPSSADNAAGRVPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGY 300

Query: 309 GNNLDCYNQRSF 321
           GNNLDCYNQRSF
Sbjct: 301 GNNLDCYNQRSF 312

BLAST of CSPI06G32350 vs. ExPASy TrEMBL
Match: A0A1L3A6A8 (Family 19 chitinase (Fragment) OS=Cucumis melo var. saccharinus OX=1922203 GN=CHT-2 PE=2 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 3.2e-173
Identity = 286/311 (91.96%), Postives = 291/311 (93.57%), Query Frame = 0

Query: 10  KTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRG 69
           KTYSL+IL FAFLL AA+AEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRG
Sbjct: 1   KTYSLIILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRG 60

Query: 70  SSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGSV 129
           SSTPTPSGGSGVGSIISESLYNQMLKYSRD RCPSNGFYTY+AFITAARSFP FG TG  
Sbjct: 61  SSTPTPSGGSGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTTGDA 120

Query: 130 ETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCASGQQY 189
            TRKRE+AAFFGQTSHETTGG  TAPDGPYAWGYCFIRERNQQ YCTPSQQWPCA GQQY
Sbjct: 121 TTRKREIAAFFGQTSHETTGGXSTAPDGPYAWGYCFIRERNQQXYCTPSQQWPCAPGQQY 180

Query: 190 YGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHNV 249
           YGRGPIQLTHNYNYGPAG AIGAPLL +PD VATD V SFKTALWFWMTAQGNKPSCHNV
Sbjct: 181 YGRGPIQLTHNYNYGPAGKAIGAPLLTNPDTVATDPVTSFKTALWFWMTAQGNKPSCHNV 240

Query: 250 IIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYG 309
           I GNWQPSSADNAAGR  GYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYG
Sbjct: 241 ITGNWQPSSADNAAGRVPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYG 300

Query: 310 NNLDCYNQRSF 321
           NNLDCYNQRSF
Sbjct: 301 NNLDCYNQRSF 311

BLAST of CSPI06G32350 vs. ExPASy TrEMBL
Match: Q9AVA8 (Endochitinase MCHT-2 OS=Cucumis melo OX=3656 GN=mcht-2 PE=2 SV=2)

HSP 1 Score: 607.4 bits (1565), Expect = 3.3e-170
Identity = 283/312 (90.71%), Postives = 289/312 (92.63%), Query Frame = 0

Query: 9   MKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 68
           MKTYSL+IL FAFLL AA+AEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR
Sbjct: 1   MKTYSLIILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 60

Query: 69  GSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGS 128
           GSSTP PSGG GVGSIISESLYNQMLKYSRD RCPSNGFYTY+AFITAARSFP FG TG 
Sbjct: 61  GSSTPPPSGGRGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTTGD 120

Query: 129 VETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCASGQQ 188
             TRKRE+AAFFGQTSHETTGGW TAPDGPYAWGY +IRERNQQTYCTPSQQWPCA GQQ
Sbjct: 121 ATTRKREIAAFFGQTSHETTGGWSTAPDGPYAWGYWYIRERNQQTYCTPSQQWPCAPGQQ 180

Query: 189 YYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHN 248
           YYGRGPIQLTHNYNYGPAG AIGAPLL +PD  ATD V SFKTALWFWMTAQGNKPSCHN
Sbjct: 181 YYGRGPIQLTHNYNYGPAGKAIGAPLLTNPD-TATDPVTSFKTALWFWMTAQGNKPSCHN 240

Query: 249 VIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGY 308
           VI GNWQPSSADNAAGR  GYGVITNIINGGLECG GPDDRVKDRIGFYKRYCDMLGIGY
Sbjct: 241 VITGNWQPSSADNAAGRVPGYGVITNIINGGLECGRGPDDRVKDRIGFYKRYCDMLGIGY 300

Query: 309 GNNLDCYNQRSF 321
           GNNLDCYNQRSF
Sbjct: 301 GNNLDCYNQRSF 311

BLAST of CSPI06G32350 vs. ExPASy TrEMBL
Match: A0A6J1GD14 (endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.2e-154
Identity = 256/314 (81.53%), Postives = 280/314 (89.17%), Query Frame = 0

Query: 9   MKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 68
           MK ++L++L FAF+L   AAEQCGRQANGALCPN LCCSQFG+CGDTD+YCK+GCQSQC 
Sbjct: 8   MKLHTLIVLAFAFVL-GGAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCG 67

Query: 69  GS--STPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNT 128
           GS  STP  SGGSGVGSIISESLYNQMLKYS+D RCPSNGFY Y+AF+TAA+SFP FG T
Sbjct: 68  GSTPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTT 127

Query: 129 GSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCASG 188
           G   TRKRE+AAFFGQTSHETTGGW TAPDGPYAWGYCFIRERNQ  YC+P+QQWPCA+G
Sbjct: 128 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAG 187

Query: 189 QQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSC 248
           Q+YYGRGPIQLTHNYNYGPAGNA+   LL +PDLVATDAVVSFKTA+WFWMT QGNKPSC
Sbjct: 188 QKYYGRGPIQLTHNYNYGPAGNALNLNLLGNPDLVATDAVVSFKTAIWFWMTPQGNKPSC 247

Query: 249 HNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGI 308
           H+VI G WQPSSAD+AAGR  GYGVITNIINGGLECGHGPDDRV DRIGFYKRY DMLG+
Sbjct: 248 HDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGV 307

Query: 309 GYGNNLDCYNQRSF 321
            YG+NLDC NQRSF
Sbjct: 308 SYGSNLDCNNQRSF 320

BLAST of CSPI06G32350 vs. NCBI nr
Match: XP_004134833.1 (endochitinase [Cucumis sativus])

HSP 1 Score: 670.6 bits (1729), Expect = 6.6e-189
Identity = 313/320 (97.81%), Postives = 314/320 (98.12%), Query Frame = 0

Query: 1   MLITSRKNMKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCK 60
           MLITSRKNMKTYSLVILCFAFLLV  AAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCK
Sbjct: 1   MLITSRKNMKTYSLVILCFAFLLVVVAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCK 60

Query: 61  NGCQSQCRGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSF 120
           NGCQSQCRGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTY+AFITAAR F
Sbjct: 61  NGCQSQCRGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYNAFITAARFF 120

Query: 121 PAFGNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQ 180
           PAFGNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQ YCTPSQQ
Sbjct: 121 PAFGNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQAYCTPSQQ 180

Query: 181 WPCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQ 240
           WPCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQ
Sbjct: 181 WPCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQ 240

Query: 241 GNKPSCHNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRY 300
           GNKPSCHNVI GNWQPSSADNAAGRS GYGVITNIINGGLECGHGPDDRVKDRIGFYKRY
Sbjct: 241 GNKPSCHNVITGNWQPSSADNAAGRSPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRY 300

Query: 301 CDMLGIGYGNNLDCYNQRSF 321
           CDMLGIGYGNNLDCYNQRSF
Sbjct: 301 CDMLGIGYGNNLDCYNQRSF 320

BLAST of CSPI06G32350 vs. NCBI nr
Match: KAA0025620.1 (family 19 chitinase [Cucumis melo var. makuwa] >TYK12495.1 family 19 chitinase [Cucumis melo var. makuwa])

HSP 1 Score: 624.0 bits (1608), Expect = 7.1e-175
Identity = 289/312 (92.63%), Postives = 293/312 (93.91%), Query Frame = 0

Query: 9   MKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 68
           MKTYSLVIL FAFLL AA+AEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR
Sbjct: 1   MKTYSLVILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 60

Query: 69  GSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGS 128
           GSSTP PSGGSGVGSIISESLYNQMLKYSRD RCPSNGFYTY+AFITAARSFP FG TG 
Sbjct: 61  GSSTPPPSGGSGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTTGD 120

Query: 129 VETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCASGQQ 188
             TRKRE+AAFFGQTSHETTGGW TAPDGPYAWGYCFIRERNQQTYCTPSQQWPCA GQQ
Sbjct: 121 ATTRKREIAAFFGQTSHETTGGWSTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCAPGQQ 180

Query: 189 YYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHN 248
           YYGRGPIQLTHNYNYGPAG AIGAPLL +PD VATD V SFKTALWFWMTAQGNKPSCHN
Sbjct: 181 YYGRGPIQLTHNYNYGPAGKAIGAPLLTNPDTVATDPVTSFKTALWFWMTAQGNKPSCHN 240

Query: 249 VIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGY 308
           VI GNWQPSSADNAAGR  GYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGY
Sbjct: 241 VITGNWQPSSADNAAGRVPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGY 300

Query: 309 GNNLDCYNQRSF 321
           GNNLDCYNQRSF
Sbjct: 301 GNNLDCYNQRSF 312

BLAST of CSPI06G32350 vs. NCBI nr
Match: APF47214.1 (family 19 chitinase, partial [Cucumis melo var. saccharinus])

HSP 1 Score: 616.7 bits (1589), Expect = 1.1e-172
Identity = 285/311 (91.64%), Postives = 291/311 (93.57%), Query Frame = 0

Query: 10  KTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRG 69
           KTYSL+IL FAFLL AA+AEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRG
Sbjct: 1   KTYSLIILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRG 60

Query: 70  SSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGSV 129
           SSTPTPSGGSGVGSIISESLYNQMLKYSRD RCPSNGFYTY+AFITAARSFP FG TG  
Sbjct: 61  SSTPTPSGGSGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTTGDA 120

Query: 130 ETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCASGQQY 189
            TRKRE+AAFFGQTSHETTGG  TAPDGPYAWGYCFIRERNQQ YCTPSQQWPCA GQQY
Sbjct: 121 TTRKREIAAFFGQTSHETTGGXSTAPDGPYAWGYCFIRERNQQXYCTPSQQWPCAPGQQY 180

Query: 190 YGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHNV 249
           YGRGPIQLTHNYNYGPAG AIGAPLL +P+ VATD V SFKTALWFWMTAQGNKPSCHNV
Sbjct: 181 YGRGPIQLTHNYNYGPAGKAIGAPLLTNPBTVATDPVTSFKTALWFWMTAQGNKPSCHNV 240

Query: 250 IIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYG 309
           I GNWQPSSADNAAGR  GYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYG
Sbjct: 241 ITGNWQPSSADNAAGRVPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYG 300

Query: 310 NNLDCYNQRSF 321
           NNLDCYNQRSF
Sbjct: 301 NNLDCYNQRSF 311

BLAST of CSPI06G32350 vs. NCBI nr
Match: NP_001284473.1 (endochitinase-like precursor [Cucumis melo] >BAB40817.2 endochitinase MCHT-2 [Cucumis melo])

HSP 1 Score: 607.4 bits (1565), Expect = 6.9e-170
Identity = 283/312 (90.71%), Postives = 289/312 (92.63%), Query Frame = 0

Query: 9   MKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 68
           MKTYSL+IL FAFLL AA+AEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR
Sbjct: 1   MKTYSLIILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 60

Query: 69  GSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGS 128
           GSSTP PSGG GVGSIISESLYNQMLKYSRD RCPSNGFYTY+AFITAARSFP FG TG 
Sbjct: 61  GSSTPPPSGGRGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTTGD 120

Query: 129 VETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCASGQQ 188
             TRKRE+AAFFGQTSHETTGGW TAPDGPYAWGY +IRERNQQTYCTPSQQWPCA GQQ
Sbjct: 121 ATTRKREIAAFFGQTSHETTGGWSTAPDGPYAWGYWYIRERNQQTYCTPSQQWPCAPGQQ 180

Query: 189 YYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHN 248
           YYGRGPIQLTHNYNYGPAG AIGAPLL +PD  ATD V SFKTALWFWMTAQGNKPSCHN
Sbjct: 181 YYGRGPIQLTHNYNYGPAGKAIGAPLLTNPD-TATDPVTSFKTALWFWMTAQGNKPSCHN 240

Query: 249 VIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGY 308
           VI GNWQPSSADNAAGR  GYGVITNIINGGLECG GPDDRVKDRIGFYKRYCDMLGIGY
Sbjct: 241 VITGNWQPSSADNAAGRVPGYGVITNIINGGLECGRGPDDRVKDRIGFYKRYCDMLGIGY 300

Query: 309 GNNLDCYNQRSF 321
           GNNLDCYNQRSF
Sbjct: 301 GNNLDCYNQRSF 311

BLAST of CSPI06G32350 vs. NCBI nr
Match: KAE8647624.1 (hypothetical protein Csa_003585 [Cucumis sativus])

HSP 1 Score: 583.6 bits (1503), Expect = 1.1e-162
Identity = 274/316 (86.71%), Postives = 291/316 (92.09%), Query Frame = 0

Query: 9   MKTYS-LVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQC 68
           MK+ S L+IL FAFLL + + EQCGRQANGALCPNNLCCSQ+G+CGDTD YCK+GCQSQC
Sbjct: 1   MKSRSCLIILAFAFLLGSTSGEQCGRQANGALCPNNLCCSQYGWCGDTDAYCKDGCQSQC 60

Query: 69  RGSSTPTP--SGGSG-VGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFG 128
           RGS++PTP   GGSG VGSII+ESLYNQMLKYS DSRCPSNGFYTY+AFITAA+SFP FG
Sbjct: 61  RGSTSPTPPSGGGSGTVGSIITESLYNQMLKYSTDSRCPSNGFYTYNAFITAAQSFPDFG 120

Query: 129 NTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCA 188
            TG   TRKRE+AAFFGQTSHETTGGW +APDGPYAWGYCFIRE NQ  YCTPS QWPC 
Sbjct: 121 TTGDDATRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPSDQWPCV 180

Query: 189 SGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKP 248
           +GQ+YYGRGP+QLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKP
Sbjct: 181 AGQKYYGRGPMQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKP 240

Query: 249 SCHNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDML 308
           SCHNVI GNWQPSSADNAAGRS GYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDML
Sbjct: 241 SCHNVITGNWQPSSADNAAGRSPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDML 300

Query: 309 GIGYGNNLDCYNQRSF 321
           GIGYGNNLDCYNQRSF
Sbjct: 301 GIGYGNNLDCYNQRSF 316

BLAST of CSPI06G32350 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 449.1 bits (1154), Expect = 2.9e-126
Identity = 210/314 (66.88%), Postives = 243/314 (77.39%), Query Frame = 0

Query: 9   MKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKN-GCQSQC 68
           MKT   + L F+ LL  ++AEQCGRQA GALCPN LCCS+FG+CG+T+ YCK  GCQSQC
Sbjct: 14  MKTNLFLFLIFSLLLSLSSAEQCGRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQSQC 73

Query: 69  RGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTG 128
               TP P     +  IIS S ++ MLK+  D+ CP+ GFYTY+AFITAA+SFP FG TG
Sbjct: 74  TPGGTP-PGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTG 133

Query: 129 SVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQT-YCTPSQQWPCASG 188
              TRK+EVAAFFGQTSHETTGGW TAPDGPY+WGYCF +E+N  + YC PS  WPCASG
Sbjct: 134 DTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCASG 193

Query: 189 QQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSC 248
           ++YYGRGP+QL+ NYNYG  G AIG  LL +PDLVA DAV++FK A+WFWMTAQ  KPSC
Sbjct: 194 KRYYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSC 253

Query: 249 HNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGI 308
           H VI G WQPS AD AAGR  GYGVITNIINGGLECG G D RV DRIGFY+RYC++ G+
Sbjct: 254 HAVIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIFGV 313

Query: 309 GYGNNLDCYNQRSF 321
             G NLDCYNQRSF
Sbjct: 314 NPGGNLDCYNQRSF 326

BLAST of CSPI06G32350 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 301.2 bits (770), Expect = 9.9e-82
Identity = 137/240 (57.08%), Postives = 175/240 (72.92%), Query Frame = 0

Query: 83  SIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGSVETRKREVAAFFGQ 142
           S++  +LY+Q+  +  ++ CP+ GFY Y AF+ A RSFP FG+ G+  TR+REVAAF  Q
Sbjct: 40  SLVPRTLYDQIFIHKDNNACPAKGFYPYEAFVEATRSFPKFGSVGNFWTRRREVAAFLAQ 99

Query: 143 TSHETTGGWPTAPDGPYAWGYCFIRERN-QQTYCTPS-QQWPCASGQQYYGRGPIQLTHN 202
            SHETTGGW TAPDGPYAWG CF  E + Q  YC  S + WPC SG+ Y GRGPIQL+ N
Sbjct: 100 ISHETTGGWATAPDGPYAWGLCFKEEVSPQSNYCDASNKDWPCVSGKSYKGRGPIQLSWN 159

Query: 203 YNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHNVIIGNWQPSSAD 262
           YNYG AG A+G   L +P+LVA ++V++FKTALWFWMT Q  KPSCHNV++  ++P+ AD
Sbjct: 160 YNYGQAGRALGFDGLQNPELVANNSVLAFKTALWFWMTEQTPKPSCHNVMVNRYRPTKAD 219

Query: 263 NAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYGNNLDCYNQRSF 321
            AA R+ GYG++TNIINGGLECG   D RV DR+G+++RY  +  +  G NLDC NQR F
Sbjct: 220 RAANRTVGYGLVTNIINGGLECGIPGDGRVTDRVGYFQRYAQLFKVTTGPNLDCENQRPF 279

BLAST of CSPI06G32350 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 293.1 bits (749), Expect = 2.7e-79
Identity = 134/244 (54.92%), Postives = 175/244 (71.72%), Query Frame = 0

Query: 79  SGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAFGNTGSVETRKREVAA 138
           + +  ++   LYN++  +  ++ CP+NGFYTY +F+ A R FP FG+ GS  T++ EVAA
Sbjct: 28  TSIERLVPRDLYNKIFIHKDNTACPANGFYTYESFVQATRRFPRFGSVGSPVTQRLEVAA 87

Query: 139 FFGQTSHETTGGWPTAPDGPYAWGYCFIRERN-QQTYCTPSQ-QWPCASGQQYYGRGPIQ 198
           F  Q SHETTGGW TAPDGPYAWG CF  E + Q TYC  S  QWPC   + Y GRGPIQ
Sbjct: 88  FLAQISHETTGGWATAPDGPYAWGLCFKEEVSPQSTYCDSSDTQWPCFPNKTYQGRGPIQ 147

Query: 199 LTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSCHNVIIGNWQP 258
           L+ NYNYGPAG A+G   L +P+ V+ ++V++F+TALWFWMT Q  KPSCH+V+IG ++P
Sbjct: 148 LSWNYNYGPAGRALGFDGLRNPETVSNNSVIAFQTALWFWMTPQSPKPSCHDVMIGKYRP 207

Query: 259 SSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYGNNLDCYN 318
           ++AD AA R+ G+G+ TNIINGGLECG   D RV DRIGF++RY  +  +  G NLDC N
Sbjct: 208 TAADLAANRTGGFGLTTNIINGGLECGIPGDGRVNDRIGFFQRYTGLFKVATGPNLDCEN 267

Query: 319 QRSF 321
           QR +
Sbjct: 268 QRPY 271

BLAST of CSPI06G32350 vs. TAIR 10
Match: AT2G43610.1 (Chitinase family protein )

HSP 1 Score: 199.1 bits (505), Expect = 5.3e-51
Identity = 122/315 (38.73%), Postives = 161/315 (51.11%), Query Frame = 0

Query: 13  SLVILCFAFLLVAAAA--EQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQS---QC 72
           +L+I  F   ++   A  + CG   NG  C  N+CCS++G+CG T  YC  GCQS     
Sbjct: 11  ALIIFLFTLTIMTGTAFSQNCG--TNG--CKGNMCCSRWGYCGTTKAYCGTGCQSGPCNS 70

Query: 73  RGSSTPTPSGGSG--------VGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARS 132
           +   TPTPSG  G        + S+I+ + +N ++     S CP+ GFYT  AFI AA S
Sbjct: 71  KPKPTPTPSGSGGLNAGPRGTIASVITPAFFNSIMS-KVGSGCPAKGFYTRQAFIAAAES 130

Query: 133 FPAFGNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQTYCTPSQ 192
           F A+  T      KRE+AA   Q SHE             +  +C+  E  +  YC+PS 
Sbjct: 131 FAAYKGT----VAKREIAAMLAQFSHE-------------SGSFCYKEEIARGRYCSPST 190

Query: 193 QWPCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTA 252
            +PC  G+ YYGRGPIQ+T NYNYG AG  +G PLL  PD+VA    V+F+ A+WFW   
Sbjct: 191 TYPCQPGKNYYGRGPIQITWNYNYGAAGKFLGLPLLKDPDMVARSPTVAFQCAMWFW--- 250

Query: 253 QGNKPSCHNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKR 312
             NK         N +P         S G+G  T  INGG EC  G    V+ R+  Y  
Sbjct: 251 --NK---------NVRPV-------LSQGFGATTRRINGG-ECNGGRPAAVQSRVNHYLD 281

Query: 313 YCDMLGIGYGNNLDC 315
           +C  LG+  G NL C
Sbjct: 311 FCKKLGVTPGTNLSC 281

BLAST of CSPI06G32350 vs. TAIR 10
Match: AT3G54420.1 (homolog of carrot EP3-3 chitinase )

HSP 1 Score: 197.6 bits (501), Expect = 1.5e-50
Identity = 118/314 (37.58%), Postives = 161/314 (51.27%), Query Frame = 0

Query: 4   TSRKNMKTYSLVILCFAFLLVAAAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGC 63
           T  K++   +++++  AF      A+ CG       C + LCCSQFGFCG+T DYC  GC
Sbjct: 5   TISKSISLVTILLVLQAF-SNTTKAQNCG-------CSSELCCSQFGFCGNTSDYCGVGC 64

Query: 64  QSQCRGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYSAFITAARSFPAF 123
           Q        P P+ G  V  I+++  +N ++  +  S C  N FY+  AF+ A  S+  F
Sbjct: 65  QQ--GPCFAPPPANGVSVAEIVTQEFFNGIISQAASS-CAGNRFYSRGAFLEALDSYSRF 124

Query: 124 GNTGSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERN--QQTYCTP-SQQ 183
           G  GS +  +RE+AAFF   +HET               +C+I E +   + YC   + Q
Sbjct: 125 GRVGSTDDSRREIAAFFAHVTHET-------------GHFCYIEEIDGASKDYCDENATQ 184

Query: 184 WPCASGQQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQ 243
           +PC   + YYGRGPIQL+ N+NYGPAG AIG   L +P+ VATD V+SFKTALW+W    
Sbjct: 185 YPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVATDPVISFKTALWYW---- 244

Query: 244 GNKPSCHNVIIGNWQPSSADNAAGRSTGYGVITNIINGGLECGHGPDDRVKDRIGFYKRY 303
                         QP         S G+G     ING LEC       V+ R+ +Y  Y
Sbjct: 245 ----------TNRVQPVI-------SQGFGATIRAINGALECDGANTATVQARVRYYTDY 273

Query: 304 CDMLGIGYGNNLDC 315
           C  LG+  GNNL C
Sbjct: 305 CRQLGVDPGNNLTC 273

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FRV11.2e-12767.74Basic endochitinase A OS=Secale cereale OX=4550 GN=rsca PE=1 SV=1[more]
Q7DNA12.6e-12767.08Chitinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht2 PE=1 SV=1[more]
P936801.3e-12667.86Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2[more]
Q429931.7e-12664.49Chitinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht1 PE=2 SV=1[more]
P082521.7e-12666.46Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KJK43.2e-18997.81Chitin-binding type-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Cs... [more]
A0A5A7SHJ53.4e-17592.63Family 19 chitinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G... [more]
A0A1L3A6A83.2e-17391.96Family 19 chitinase (Fragment) OS=Cucumis melo var. saccharinus OX=1922203 GN=CH... [more]
Q9AVA83.3e-17090.71Endochitinase MCHT-2 OS=Cucumis melo OX=3656 GN=mcht-2 PE=2 SV=2[more]
A0A6J1GD141.2e-15481.53endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004134833.16.6e-18997.81endochitinase [Cucumis sativus][more]
KAA0025620.17.1e-17592.63family 19 chitinase [Cucumis melo var. makuwa] >TYK12495.1 family 19 chitinase [... [more]
APF47214.11.1e-17291.64family 19 chitinase, partial [Cucumis melo var. saccharinus][more]
NP_001284473.16.9e-17090.71endochitinase-like precursor [Cucumis melo] >BAB40817.2 endochitinase MCHT-2 [Cu... [more]
KAE8647624.11.1e-16286.71hypothetical protein Csa_003585 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G12500.12.9e-12666.88basic chitinase [more]
AT4G01700.19.9e-8257.08Chitinase family protein [more]
AT1G02360.12.7e-7954.92Chitinase family protein [more]
AT2G43610.15.3e-5138.73Chitinase family protein [more]
AT3G54420.11.5e-5037.58homolog of carrot EP3-3 chitinase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001002Chitin-binding, type 1PRINTSPR00451CHITINBINDNGcoord: 38..46
score: 66.37
coord: 46..53
score: 75.0
coord: 53..60
score: 57.5
IPR001002Chitin-binding, type 1SMARTSM00270ChitinBD_3coord: 30..67
e-value: 2.0E-16
score: 70.5
IPR001002Chitin-binding, type 1PFAMPF00187Chitin_bind_1coord: 30..67
e-value: 4.3E-15
score: 55.9
IPR001002Chitin-binding, type 1PROSITEPS50941CHIT_BIND_I_2coord: 28..69
score: 10.821191
NoneNo IPR availableGENE3D3.30.20.10Endochitinase, domain 2coord: 167..223
e-value: 1.9E-117
score: 392.9
NoneNo IPR availableGENE3D1.10.530.10coord: 84..315
e-value: 1.9E-117
score: 392.9
NoneNo IPR availablePIRSRPIRSR001060-2PIRSR001060-2coord: 8..320
e-value: 7.7E-144
score: 475.8
NoneNo IPR availablePANTHERPTHR22595CHITINASE-RELATEDcoord: 9..320
NoneNo IPR availablePANTHERPTHR22595:SF171CHITINASE (CLASS IB) / HEVEINcoord: 9..320
NoneNo IPR availableCDDcd06921ChtBD1_GH19_heveincoord: 29..67
e-value: 8.70612E-17
score: 70.9812
NoneNo IPR availableCDDcd00325chitinase_GH19coord: 85..314
e-value: 1.61593E-111
score: 320.916
IPR016283Glycoside hydrolase, family 19PIRSFPIRSF001060Endochitinasecoord: 5..320
e-value: 1.7E-126
score: 418.6
IPR000726Glycoside hydrolase, family 19, catalyticPFAMPF00182Glyco_hydro_19coord: 84..314
e-value: 1.9E-125
score: 417.2
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00774CHITINASE_19_2coord: 227..237
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00773CHITINASE_19_1coord: 102..124
IPR036861Endochitinase-like superfamilyGENE3D3.30.60.10coord: 28..70
e-value: 6.1E-18
score: 66.7
IPR036861Endochitinase-like superfamilySUPERFAMILY57016Plant lectins/antimicrobial peptidescoord: 30..73
IPR018371Chitin-binding, type 1, conserved sitePROSITEPS00026CHIT_BIND_I_1coord: 40..59
IPR023346Lysozyme-like domain superfamilySUPERFAMILY53955Lysozyme-likecoord: 81..320

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G32350.1CSPI06G32350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016998 cell wall macromolecule catabolic process
biological_process GO:0006032 chitin catabolic process
biological_process GO:0050832 defense response to fungus
molecular_function GO:0004568 chitinase activity
molecular_function GO:0008061 chitin binding