CSPI06G27140 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G27140
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	iron-sulfur assembly protein IscA, chloroplastic-like
Location	Chr6: 23921574 .. 23924855 (-)
RNA-Seq Expression	CSPI06G27140
Synteny	CSPI06G27140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAAAATACAAAAGAAGAGAAGATGAAGAAAAATATCTGAAACGCTCGATCTTAACGAAAACAATATATCTTGACCGCACGATCCCAATACCCAAACATTTTCGACTAGTCAAACACGGCGGTACGCATATTCACTGGTCGTCGTTCACCCTTCGCCGGAAATATCGCGGCAGCACAACCGGGTTTCTCCGATGGCTTTCTCTTCGATTTCCATGAAATGCCCTTGCCCTCCCGTCCTTCACTTGCCAGCTCTTCCTAAAAGTTATGCACCTCAAACTTCTATTTCCTTTCGTTTTCCTTCTACGAAATTCAGCCGGAGAAAATCCTTATCGATTCGATCGGTTTCAGTCCCAGGTACGCTCTAATTTTCAATTTTCCCATCTGTTTTCCGTTATTATACTTGGCTATATCACCTTAGTTCATCGTTGTACTACTTGGGACGAAATTAATCAGGGTTTTGTTTTTCCCTTTTGGATTGGATTGAAATTTGAGGTTGTGGAGAACGTCTGGATTAGAGTTATTTTTAAAAAAAATCAATCAAACGTAGGATTCTTTTGTTGGTTGATTGTAATCGAAATTGATAGCTGGTGGATGTCTTGTGTTGAAATTAAGTAACAGAATTTTGGGACTTTATTGTTGATTTTCATGGAAAATAAATTTCCCCTGTACTTGTACTGACGACTTGTTTATGAGTTATAGTTCAAGATCCAGTTGGTGATATACAAACCGTGAATTTTAGGTCTTTGTTTTGTTCTAATGCCTAAATGATTAGTAGGATGGATTGGCCTTCATGTGGAGGCAAAGGTGTTTACTTTTTCTTGTGTTTGATACCATTGATTGGTTGTTTGTTTTAGAGCCTGTTTGGGAGTGGTTTTGAAATGGTTAATGGCTTTCTTTCATGTTCAAAACCACTCCGGAATTTGTCTTTAATCATTAAAAAAAGATTTAATGTTCGATAATTTCACTTTTAAATGCAATTTCCATATCTCTAAATTTAATTTTGAAGGATTAAAAGCATATTTCAAGTCATTTTGGATATGAAAAGTGATTTTAACCCTTTCAAAATAAGTCCAAACATGCCCTTTTATATCTTCTGGTGCAAATTATTTGTTGTTTTCTCTATGTCACTTTCCCTTTGCCTGTTACTGCAGATCAAAAGATAAAACCTAATGAATCTTGTCCTATAGTTTTGGCATATGAAATACGATGAGTATATCATGCAGTTATGATCAAAGTTTGCCTCGTCATCTTATGTTTTTTGTAATTAAACAGCTGCGCCAGCATCTGAGGGTATAGCACCTGCTATCTCATTAACCGATACTGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTAAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCTTACACCATGGAGTTTGAGAGCAGGGCAAATGCTAGACCTGATGACTCGATTATAGAATATAATGGCTTTGTGATAGGTAAGAGTTATGGCTTTGCTATTTTTCACCTTGAATTTCTAGTGAGATTTTTACTAGTGATAGTGCAACTGTTTGAAGCAATCCTTTTTTTGAACCCATTGCTTTATATACATGTGTATGTGTGTGGGTGGGTGGGGGAGGGGGGTGTGAGGATAGTGGAAAGTCCAAGTCTTGTTTTGAACATGTGTTGAATTTTGATACTGTGGAATGGTAAGGGCAAGTTTTCAAATTTCCTTAGGATGAAAGAGGCTTACTCTTTTATAGTTCTATACATTCAGATAGTGATGGATATTTCATGAACCATATGCCATAAATTTGAAGGACTCTGCAAGAATAATTTTTCTCTATAGTTCATAATCTACCTCTTAGCTCTAAAAGAAAACGGTGGTATACATAAGTCTAGGTTTGCATTTTTTTTATGAATCAAACTGCAAAGAATACAAGGGCGTGAAAAAAATCCAGCCCACCAAAACACCCCAACCATCGTAAGGAGGACCAACTAAGTAAAATGTTACCAATAGAGTAATTACAAAAATGCTTTGATATTGAAGCCGACTATGCTCCTTCTCCCTACCTCTAAAGACTCGATCGTTCCTCTCACCCCAAATGTCTCAAACAATAGTACACACCCCAACCAACAATAAAAATCCCCCTTTATCATTGAAAGGCAGATGGAGGAGGAACTCCTTGATCGTTGCTCTGTCACTCCTAGAGCCAACAAAACAAACACCCAACTCCTGCAGGAAAGAACTCCAAACTGCCCTTGCATAATGAGAGTCCCAGAAAAGAAGTCTAGGTTTGCATATTGTTAGATTGATGCTTTCTTTCCCGTGGATCGGTAGAGGACAAGTTTTCAATTTTTATTAGGATGAAAGAAGCTTACATATCATGAGCTGCGTGCCATAAATTTGCTGGACTCTATAAGAAGTTTTACTCTATAGCTCATAATCTATCACTTCTCTTCGACACTCAATCACTTGAATTATTTGATATGAGCAATTTTTTATGCTAACTGATAAGTAGGTTGCTCGACAACTTTTGTCAAATATATTTTTCTTTAATGCATGCTATTCAACATAAAGTGGTATATTGTTTCAATTCCTTGATAATTGCCAAAGTGATAAAAAAGTTTGTAATTCAAAACTTCGAGTCCTTCCACCCTCTTACCAATGAAAAGAGTGTTCTTGATAATTGCTCTCTACTCTATTGATAAAGGAAAGTTAGCATCTTTTAGTGAACTATGAAAGGATTTTTTTATCTACTTCATTTTCCTCTATGCCCTTTTAAGTTGTACCCTCGAACACGAATTTAAGTTCACTTTTAGTTTGCACTTTTGATACAAATTTCCCTCTCTTCTTGTTTTGTAGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGAGGATTTTCTTTCAAGAACCCAAATGCTACACAAACTTGTGGTTGTGGAAAATCATTTAATGCAGAAATGTAGGACCATATTTTGTTTATATGAATGATTGAATAGAAACTTGATAGTTTCAGGTTAAAGAAAACTTAGAATGTATAAAAACTCTTACATTGCTTGACAATTGATCCTAATAGTATACCTTCTATTACTAGTCGTTGGTGATATGTCTGCTTTTGCCCTTCTGGAAAAGGGAATTGGCCTTTTAGCTCTTCCTGCTCACTTTCTTACAGATTCAATGCACGTCTTTATCAAAAACATAGGCTAAACTATATTATCTAAAAGTTGATGTTTAAGAGGTATCCGTC

mRNA sequence

TAAAAATACAAAAGAAGAGAAGATGAAGAAAAATATCTGAAACGCTCGATCTTAACGAAAACAATATATCTTGACCGCACGATCCCAATACCCAAACATTTTCGACTAGTCAAACACGGCGGTACGCATATTCACTGGTCGTCGTTCACCCTTCGCCGGAAATATCGCGGCAGCACAACCGGGTTTCTCCGATGGCTTTCTCTTCGATTTCCATGAAATGCCCTTGCCCTCCCGTCCTTCACTTGCCAGCTCTTCCTAAAAGTTATGCACCTCAAACTTCTATTTCCTTTCGTTTTCCTTCTACGAAATTCAGCCGGAGAAAATCCTTATCGATTCGATCGGTTTCAGTCCCAGCTGCGCCAGCATCTGAGGGTATAGCACCTGCTATCTCATTAACCGATACTGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTAAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCTTACACCATGGAGTTTGAGAGCAGGGCAAATGCTAGACCTGATGACTCGATTATAGAATATAATGGCTTTGTGATAGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGAGGATTTTCTTTCAAGAACCCAAATGCTACACAAACTTGTGGTTGTGGAAAATCATTTAATGCAGAAATGTAGGACCATATTTTGTTTATATGAATGATTGAATAGAAACTTGATAGTTTCAGGTTAAAGAAAACTTAGAATGTATAAAAACTCTTACATTGCTTGACAATTGATCCTAATAGTATACCTTCTATTACTAGTCGTTGGTGATATGTCTGCTTTTGCCCTTCTGGAAAAGGGAATTGGCCTTTTAGCTCTTCCTGCTCACTTTCTTACAGATTCAATGCACGTCTTTATCAAAAACATAGGCTAAACTATATTATCTAAAAGTTGATGTTTAAGAGGTATCCGTC

Coding sequence (CDS)

ATGGCTTTCTCTTCGATTTCCATGAAATGCCCTTGCCCTCCCGTCCTTCACTTGCCAGCTCTTCCTAAAAGTTATGCACCTCAAACTTCTATTTCCTTTCGTTTTCCTTCTACGAAATTCAGCCGGAGAAAATCCTTATCGATTCGATCGGTTTCAGTCCCAGCTGCGCCAGCATCTGAGGGTATAGCACCTGCTATCTCATTAACCGATACTGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTAAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCTTACACCATGGAGTTTGAGAGCAGGGCAAATGCTAGACCTGATGACTCGATTATAGAATATAATGGCTTTGTGATAGTATGTGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGAGGATTTTCTTTCAAGAACCCAAATGCTACACAAACTTGTGGTTGTGGAAAATCATTTAATGCAGAAATGTAG

Protein sequence

MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASEGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM*

Homology

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BLAST of CSPI06G27140 vs. ExPASy Swiss-Prot
Match: Q9XIK3 (Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISCA PE=2 SV=2)

HSP 1 Score: 238.0 bits (606), Expect = 7.9e-62
Identity = 116/148 (78.38%), Postives = 127/148 (85.81%), Query Frame = 0


Query: 32  SFRFPSTKF-----SRRKSLSIRSVSVPAAPASEGIAPAISLTDTALKHLNKMRSERNED 91
           S  FPS  +     +RR  LS+RS SVPAAPA EG+ PAISL++ ALKHL+KMRSER ED
Sbjct: 33  SISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGED 92

Query: 92  LCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSD 151
           LCLRIGV+QGGCSGMSYTM+FE+RANARPDDS IEY GF IVCDPKS+LFLFGMQLDYSD
Sbjct: 93  LCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSD 152

Query: 152 ALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           ALIGGGFSF NPNATQTCGCGKSF AEM
Sbjct: 153 ALIGGGFSFSNPNATQTCGCGKSFAAEM 180

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BLAST of CSPI06G27140 vs. ExPASy Swiss-Prot
Match: P72731 (Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1417 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.5e-36
Identity = 68/108 (62.96%), Postives = 83/108 (76.85%), Query Frame = 0


Query: 63  APAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEY 122
           A  I L+D ALKHL  ++ ++ +DLCLR+GVRQGGCSGMSY M+FE    A   D + +Y
Sbjct: 9   AKGIQLSDAALKHLLALKEQQGKDLCLRVGVRQGGCSGMSYMMDFEEPNRATEHDEVFDY 68

Query: 123 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 171
            GF I+CD KSLL+L+G+ LDYS+ALIGGGF F NPNA QTCGCGKSF
Sbjct: 69  EGFQIICDRKSLLYLYGLMLDYSNALIGGGFQFTNPNANQTCGCGKSF 116

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BLAST of CSPI06G27140 vs. ExPASy Swiss-Prot
Match: P51217 (Uncharacterized protein ycf83 OS=Porphyra purpurea OX=2787 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.0e-32
Identity = 60/106 (56.60%), Postives = 83/106 (78.30%), Query Frame = 0


Query: 66  ISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNGF 125
           I+LT+ A + +  ++S  + ++CLRIGV+QGGCSGMSY+M FES ++ R  D ++  + F
Sbjct: 8   ITLTEPAARQIKILKSNFDNEVCLRIGVKQGGCSGMSYSMNFESVSSLRETDEVLMLDSF 67

Query: 126 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFN 172
           ++ CDPKSLL+L+G+ LDYS  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 68  LVACDPKSLLYLYGLSLDYSSELIGGGFQFSNPNASQTCGCGKSFS 113

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BLAST of CSPI06G27140 vs. ExPASy Swiss-Prot
Match: Q1XDR9 (Uncharacterized protein ycf83 OS=Pyropia yezoensis OX=2788 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.6e-30
Identity = 61/112 (54.46%), Postives = 80/112 (71.43%), Query Frame = 0


Query: 60  EGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSI 119
           EG    I++T  ALK +  ++++   D+ LRIGVRQGGCSGMSY+M FE     +  D  
Sbjct: 2   EGNMGFITITKPALKQIAILKNDHENDVHLRIGVRQGGCSGMSYSMNFEHVDKLKDTDER 61

Query: 120 IEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFN 172
           +  + F +VCDPKSLL+L+G+ LD+S  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 62  LRLDNFSVVCDPKSLLYLYGLSLDFSSELIGGGFQFSNPNASQTCGCGKSFS 113

[Close]

BLAST of CSPI06G27140 vs. ExPASy Swiss-Prot
Match: Q9MSA1 (Uncharacterized protein ycf83 OS=Galdieria sulphuraria OX=130081 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.1e-30
Identity = 58/107 (54.21%), Postives = 81/107 (75.70%), Query Frame = 0


Query: 66  ISLTDTALKHLNKMRSERNED-LCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNG 125
           I++T +AL HLN +    +ED L +RIG++QGGCSG+SY M +E ++N   +D + +Y+ 
Sbjct: 6   INITKSALDHLNSISKLNSEDKLYIRIGIKQGGCSGLSYFMTYEKKSNISDNDLVYDYDN 65

Query: 126 FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFN 172
           F +VCD KS+L+L+G+ LDYS +LI GGF F NPNA QTCGCGKSF+
Sbjct: 66  FTLVCDNKSILYLYGISLDYSSSLIDGGFKFLNPNAKQTCGCGKSFS 112

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BLAST of CSPI06G27140 vs. ExPASy TrEMBL
Match: A0A0A0KJ66 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486950 PE=3 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.1e-93
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 0


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE
Sbjct: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII
Sbjct: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 174

[Close]

BLAST of CSPI06G27140 vs. ExPASy TrEMBL
Match: A0A6J1GEF0 (iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111453419 PE=3 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.3e-83
Identity = 161/174 (92.53%), Postives = 166/174 (95.40%), Query Frame = 0


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSI+MK  CPPVLHL ALPKS APQ+SISFR+  TKFSRRK+LSIRSVSVPAAPASE
Sbjct: 1   MAFSSITMK--CPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           GIAPAISLTD ALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  GIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of CSPI06G27140 vs. ExPASy TrEMBL
Match: A0A6J1ISS5 (iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478089 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.2e-82
Identity = 159/174 (91.38%), Postives = 164/174 (94.25%), Query Frame = 0


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSI+MK  CPPVLHL ALPKS APQ+SISFR+  TKFSRRK+LSIRSVSVPAAPASE
Sbjct: 1   MAFSSITMK--CPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           GIAPAISLTD ALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFE+R NARPDDSII
Sbjct: 61  GIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYN FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 EYNEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of CSPI06G27140 vs. ExPASy TrEMBL
Match: A0A1S3B105 (iron-sulfur assembly protein IscA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484688 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 5.4e-82
Identity = 160/174 (91.95%), Postives = 164/174 (94.25%), Query Frame = 0


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSI+MK   PPVLHL ALPKS APQ+SISFRF  TKFSR KSLSIRSVSVPAAPASE
Sbjct: 1   MAFSSITMK--FPPVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
            IAPAISLTD ALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  SIAPAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

[Close]

BLAST of CSPI06G27140 vs. ExPASy TrEMBL
Match: A0A6J1BTR2 (iron-sulfur assembly protein IscA, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005271 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 8.1e-78
Identity = 152/174 (87.36%), Postives = 160/174 (91.95%), Query Frame = 0


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSS +MK   P VLHL ALP+S A Q+SISFR+  TKFSRRKSLS+RSVSVPAAP SE
Sbjct: 1   MAFSSATMK--FPSVLHLRALPESSATQSSISFRYSLTKFSRRKSLSVRSVSVPAAPTSE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           GIAPAISLTD AL HLNKMRS+RNEDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  GIAPAISLTDNALNHLNKMRSDRNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF A+M
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAADM 172

[Close]

BLAST of CSPI06G27140 vs. NCBI nr
Match: XP_004141965.1 (iron-sulfur assembly protein IscA, chloroplastic [Cucumis sativus] >KGN48422.1 hypothetical protein Csa_003861 [Cucumis sativus])

HSP 1 Score: 352.4 bits (903), Expect = 2.2e-93
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 0


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE
Sbjct: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII
Sbjct: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 174

[Close]

BLAST of CSPI06G27140 vs. NCBI nr
Match: XP_022950286.1 (iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata] >XP_023544833.1 iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6604246.1 Iron-sulfur assembly protein IscA, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 318.9 bits (816), Expect = 2.7e-83
Identity = 161/174 (92.53%), Postives = 166/174 (95.40%), Query Frame = 0


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSI+MK  CPPVLHL ALPKS APQ+SISFR+  TKFSRRK+LSIRSVSVPAAPASE
Sbjct: 1   MAFSSITMK--CPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           GIAPAISLTD ALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  GIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of CSPI06G27140 vs. NCBI nr
Match: KAG7034403.1 (Iron-sulfur assembly protein IscA, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 317.4 bits (812), Expect = 7.8e-83
Identity = 160/174 (91.95%), Postives = 166/174 (95.40%), Query Frame = 0


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSI++K  CPPVLHL ALPKS APQ+SISFR+  TKFSRRK+LSIRSVSVPAAPASE
Sbjct: 1   MAFSSITVK--CPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           GIAPAISLTD ALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  GIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of CSPI06G27140 vs. NCBI nr
Match: XP_022977954.1 (iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 313.9 bits (803), Expect = 8.6e-82
Identity = 159/174 (91.38%), Postives = 164/174 (94.25%), Query Frame = 0


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSI+MK  CPPVLHL ALPKS APQ+SISFR+  TKFSRRK+LSIRSVSVPAAPASE
Sbjct: 1   MAFSSITMK--CPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
           GIAPAISLTD ALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFE+R NARPDDSII
Sbjct: 61  GIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYN FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 EYNEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of CSPI06G27140 vs. NCBI nr
Match: XP_008440129.1 (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo])

HSP 1 Score: 313.5 bits (802), Expect = 1.1e-81
Identity = 160/174 (91.95%), Postives = 164/174 (94.25%), Query Frame = 0


Query: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60
           MAFSSI+MK   PPVLHL ALPKS APQ+SISFRF  TKFSR KSLSIRSVSVPAAPASE
Sbjct: 1   MAFSSITMK--FPPVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120
            IAPAISLTD ALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  SIAPAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

[Close]

BLAST of CSPI06G27140 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 238.0 bits (606), Expect = 5.6e-63
Identity = 116/148 (78.38%), Postives = 127/148 (85.81%), Query Frame = 0


Query: 32  SFRFPSTKF-----SRRKSLSIRSVSVPAAPASEGIAPAISLTDTALKHLNKMRSERNED 91
           S  FPS  +     +RR  LS+RS SVPAAPA EG+ PAISL++ ALKHL+KMRSER ED
Sbjct: 33  SISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGED 92

Query: 92  LCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSD 151
           LCLRIGV+QGGCSGMSYTM+FE+RANARPDDS IEY GF IVCDPKS+LFLFGMQLDYSD
Sbjct: 93  LCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSD 152

Query: 152 ALIGGGFSFKNPNATQTCGCGKSFNAEM 175
           ALIGGGFSF NPNATQTCGCGKSF AEM
Sbjct: 153 ALIGGGFSFSNPNATQTCGCGKSFAAEM 180

[Close]

BLAST of CSPI06G27140 vs. TAIR 10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.3e-14
Identity = 45/136 (33.09%), Postives = 67/136 (49.26%), Query Frame = 0


Query: 40  FSRRKSLSIRSVSVPAAPASEGIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCS 99
           FS   ++   S S  + P S      + L+D  ++ + +++S   E   LR+GV  GGCS
Sbjct: 26  FSSASAIKEASSSSSSQPESSS-NDVVHLSDNCIRRMKELQSSEPEKKMLRLGVETGGCS 85

Query: 100 GMSYTMEFESRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFK-NP 159
           G  Y  E ++R N  PDD + E NG  +V D  S   + G  +DY + LI   F    NP
Sbjct: 86  GFQYKFELDNRTN--PDDRVFEKNGVKLVVDNVSYDLVKGATIDYEEELIRAAFVVAVNP 145

Query: 160 NATQTCGCGKSFNAEM 175
           +A   C C  SF  ++
Sbjct: 146 SAVGGCSCKSSFMVKL 158

[Close]

BLAST of CSPI06G27140 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 72.8 bits (177), Expect = 3.1e-13
Identity = 35/105 (33.33%), Postives = 61/105 (58.10%), Query Frame = 0


Query: 66  ISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSIIEYNGF 125
           ++L+DTA   + ++   + +   LR+ V   GC+G+SY + +         D ++E  G 
Sbjct: 6   LALSDTAAARIRQLLQHQQKPF-LRLAVEAKGCNGLSYVLNYAQEKG--KFDEVVEEKGV 65

Query: 126 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 171
            I+ DPK+++ + G ++D+ D  +   F F NPNAT+ CGCG+SF
Sbjct: 66  KILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNATK-CGCGESF 106

The following BLAST results are available for this feature:

BLAST of CSPI06G27140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XIK3	7.9e-62	78.38	Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
P72731	1.5e-36	62.96	Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ...	[more]
P51217	1.0e-32	56.60	Uncharacterized protein ycf83 OS=Porphyra purpurea OX=2787 GN=ycf83 PE=3 SV=1	[more]
Q1XDR9	1.6e-30	54.46	Uncharacterized protein ycf83 OS=Pyropia yezoensis OX=2788 GN=ycf83 PE=3 SV=1	[more]
Q9MSA1	6.1e-30	54.21	Uncharacterized protein ycf83 OS=Galdieria sulphuraria OX=130081 GN=ycf83 PE=3 S...	[more]

BLAST of CSPI06G27140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KJ66	1.1e-93	100.00	Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486950...	[more]
A0A6J1GEF0	1.3e-83	92.53	iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita moschata OX=3...	[more]
A0A6J1ISS5	4.2e-82	91.38	iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita maxima OX=366...	[more]
A0A1S3B105	5.4e-82	91.95	iron-sulfur assembly protein IscA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1BTR2	8.1e-78	87.36	iron-sulfur assembly protein IscA, chloroplastic isoform X1 OS=Momordica charant...	[more]

BLAST of CSPI06G27140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141965.1	2.2e-93	100.00	iron-sulfur assembly protein IscA, chloroplastic [Cucumis sativus] >KGN48422.1 h...	[more]
XP_022950286.1	2.7e-83	92.53	iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata] >XP_0...	[more]
KAG7034403.1	7.8e-83	91.95	Iron-sulfur assembly protein IscA, chloroplastic [Cucurbita argyrosperma subsp. ...	[more]
XP_022977954.1	8.6e-82	91.38	iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita maxima]	[more]
XP_008440129.1	1.1e-81	91.95	PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo]	[more]

BLAST of CSPI06G27140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G10500.1	5.6e-63	78.38	chloroplast-localized ISCA-like protein	[more]
AT5G03905.1	1.3e-14	33.09	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	3.1e-13	33.33	Iron-sulphur cluster biosynthesis family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035903	HesB-like domain superfamily	GENE3D	2.60.300.12		coord: 65..173 e-value: 1.3E-35 score: 123.8
IPR035903	HesB-like domain superfamily	SUPERFAMILY	89360	HesB-like domain	coord: 65..161
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 66..171 e-value: 4.9E-37 score: 124.3
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 66..168 e-value: 4.1E-22 score: 78.5
None	No IPR available	PANTHER	PTHR47265:SF1	IRON-SULFUR ASSEMBLY PROTEIN ISCA, CHLOROPLASTIC	coord: 1..174
IPR031108	FeS assembly protein IscA, plant/cyanobacteria type	PANTHER	PTHR47265	IRON-SULFUR ASSEMBLY PROTEIN ISCA, CHLOROPLASTIC	coord: 1..174
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 153..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G27140.1	CSPI06G27140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0030674	protein-macromolecule adaptor activity