CSPI06G26780 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G26780
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionWAT1-related protein
LocationChr6: 23586789 .. 23589399 (+)
RNA-Seq ExpressionCSPI06G26780
SyntenyCSPI06G26780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCATAAAAATCTCAACAAACAAATGATATTTTGTCTAATGAAATATGCTATGCATTTTAAAATCCATACTCCAATATGAGTGTTTGGAAATGGGAAAACCATATGCTATTTTGAACATACTTGATTATGTACTTCAACTCCTAACACATTTGTGATGATCTTAAAATATTGGTTTTGATGATATGGAACAAAAAATGAATTCAAAGAAGCCATATTTTGCTGCACTCCTTTGTCAAACAATATTTGCAGGGATGAGTTTACTCTCAAAAGCTTCCTATTCTTCGGGTATGAATACTTTTATTTTCTTTTTCTACCGGCAAGCGGTTGGCACTATCTTCTTACTTCCCCTCACCATTTATTTCTCAAGGTAAAATACTCCATATTATTACTAAATTCATTTCCTAACTCCTTCTATTTTTATTTTATTATCCTTTAATTAACTACAATTGTGTACTCACGAGTCTGTAGTCATTTTTTTTTGGAGAAACCAAAACCAATTGAGAAAATAGCTAAAAAAAACAGATCTAACCGATTTGTTTGTTTTTGTTTTCATGGATGATTGCTGTAGAAATGAAATGGCAAGTTTATCAAAAGGAGATCTGTTGAAGATTTTCATGCAGGCCTTCTTAGGGTTTGTACCATTAATTATATATTGCAAAGTTGTTGAATTCTCTTAATTTTATTAAGATTATATTTAATTTGATTGAAAATTTTACTTGCGTAGATGAAATTAAAGTGCAGGTTTACGTTTGGTTTTAATGCTTATGGATTAGGGGTTAAGTATACATCGGCCACCTTAGGAGCTGCTGCATTTAATTGTATTCCTGTCACCACGTTCTTCTTTGCTTTTATTTTAAGGTTCGTTAACATTATATTATTTGAATTTATTTACTTTTCTCCTAAATAATTTAAAGAAACAATTCAAAACTATATCTTAATAAAAGTTATAGGCAAACTAACAAAACTCATCATCATGTAGGTCTCTGCTTTTTTAAAATTAAGGTATCTTTGCCTATATTTTTGTTTTACTTTTGAAAATTTGTTTAATCCACCCTCGTTAAGATACTTCAAAATTCAAAGAATATGTTCTGAGAACTACAAAGAGTAAGTTTGTTATATTTCTTTTTATTTTTTTAATTAAGAAATCAAGTGCTACTTAAGAAAATATATAAAAATCATTATATAAAGAACGGTGAGAAAAATAGGCATAATTTTTAAAATAGAAATGATTACAAGATGAATTTAAATTCCTAGTTTTTTGAAATTGGAATTTAGAATTTGGCAAATCGTATTGGTAAAAAGTAGTAATTCTGATTAACCATCCAAAATGGGTAAGAAGTTTATACCAAATGCACTACTTTAATTAAATCAATAACTTCTAAAAGAAAATAATAGAATATCTAATTGGTTTGGTTATTCAAGCCCTGAATAATGCAACTTGTATGGATAAATTATTCATAAATTACTAGTTGAAGTGAATGAAGATAAAATAACATTTAAGAAGTTTGAACTCAAGATCTCCAGTATGGACTAACCTACTCTAGTATCATTTTAATTGATCCAAAAACATAAACTCTATCGATATATATTTGGTATTGATAGGGTGGAGAAAGTAAATATAAAGAAAGCAAGTGGAATGGCAAAGGTTGGAGGCATAATGTTGTGCATTACTGGTGTTGCGGTTATTACATTTTACAAAGGTCCTTATTTAAAACCACTCAACAACGTTCACCTTTTTCAAACCCAACAAGCTCATGTTTCTTCCAAAAAAGAATGGATTCTTGGCTGTTCCTTGCTCCTCCTTTCTTCCCTTGCAGTGGGTTTGTGGTTTGTTCTCCAGGTTAATTTTCAACATTATCATGAATCATGTGTGGTTTATGTTATTGTTGTATGAGTAATCCTTTGATATAATATTTTATTTTCAGGTTTGGGTTTTGAGAACTTGTCCATCTCCACTTGTAGTAACATTTGGACAGACATTTTCAAGTAGCATTCAGTCCTTTGTTGTGGCTATTGCAATCGAAAGAAACCCATCTCAATGGAAGCTGGCTTGGAACATTTCCCTAGCTGCAATTCTTTATTGTGTATGATTTCTTATTTAACAATATACGGAGAATGCGGATTTGAACCTCTAGCTAACTATTAATTTGAGTCATTCAAATAGATAGTACTTATCGATTACTTCTAAACTATAATCTTCTTTTATTGTGCAGGGAGTTTTTGTAGTTTCCATTGGAAACTATTTGTCAAGCTGGGTGGTTAAGAAAAAGGGTCCAGTTTTTCAAGCTGTTACTACACCCTTCAATCTCATTGTTACACTTATTGGTTCAGAATTTCTTTTAAAGGATGGAATTTGCTTGGGAAGGTAATTATGAAATTAATTGATGGATTATTCATTGTTAATGATTTAAAATTGTTTAAACCAAATATTTGACTTTTTTGTTTTTGGTGGGTGAACAGCGGAATTGGTGCTATATTGCTAGTTTTGAGTCTTTATAGTGTTTTATGGGGAAAAAAGAAGGAAGCATGTTGTCGTGATGCATCAAACAACAACAGCATCACCAATTCTGTTCAAGTAGAAAGGGAAATATTAGATAACATTTAAATT

mRNA sequence

ATGTCATAAAAATCTCAACAAACAAATGATATTTTGTCTAATGAAATATGCTATGCATTTTAAAATCCATACTCCAATATGAGTGTTTGGAAATGGGAAAACCATATGCTATTTTGAACATACTTGATTATGTACTTCAACTCCTAACACATTTGTGATGATCTTAAAATATTGGTTTTGATGATATGGAACAAAAAATGAATTCAAAGAAGCCATATTTTGCTGCACTCCTTTGTCAAACAATATTTGCAGGGATGAGTTTACTCTCAAAAGCTTCCTATTCTTCGGGTATGAATACTTTTATTTTCTTTTTCTACCGGCAAGCGGTTGGCACTATCTTCTTACTTCCCCTCACCATTTATTTCTCAAGAAATGAAATGGCAAGTTTATCAAAAGGAGATCTGTTGAAGATTTTCATGCAGGCCTTCTTAGGGTTTACGTTTGGTTTTAATGCTTATGGATTAGGGGTTAAGTATACATCGGCCACCTTAGGAGCTGCTGCATTTAATTGTATTCCTGTCACCACGTTCTTCTTTGCTTTTATTTTAAGGGTGGAGAAAGTAAATATAAAGAAAGCAAGTGGAATGGCAAAGGTTGGAGGCATAATGTTGTGCATTACTGGTGTTGCGGTTATTACATTTTACAAAGGTCCTTATTTAAAACCACTCAACAACGTTCACCTTTTTCAAACCCAACAAGCTCATGTTTCTTCCAAAAAAGAATGGATTCTTGGCTGTTCCTTGCTCCTCCTTTCTTCCCTTGCAGTGGGTTTGTGGTTTGTTCTCCAGGTTTGGGTTTTGAGAACTTGTCCATCTCCACTTGTAGTAACATTTGGACAGACATTTTCAAGTAGCATTCAGTCCTTTGTTGTGGCTATTGCAATCGAAAGAAACCCATCTCAATGGAAGCTGGCTTGGAACATTTCCCTAGCTGCAATTCTTTATTGTGGAGTTTTTGTAGTTTCCATTGGAAACTATTTGTCAAGCTGGGTGGTTAAGAAAAAGGGTCCAGTTTTTCAAGCTGTTACTACACCCTTCAATCTCATTGTTACACTTATTGGTTCAGAATTTCTTTTAAAGGATGGAATTTGCTTGGGAAGCGGAATTGGTGCTATATTGCTAGTTTTGAGTCTTTATAGTGTTTTATGGGGAAAAAAGAAGGAAGCATGTTGTCGTGATGCATCAAACAACAACAGCATCACCAATTCTGTTCAAGTAGAAAGGGAAATATTAGATAACATTTAAATT

Coding sequence (CDS)

ATGGAACAAAAAATGAATTCAAAGAAGCCATATTTTGCTGCACTCCTTTGTCAAACAATATTTGCAGGGATGAGTTTACTCTCAAAAGCTTCCTATTCTTCGGGTATGAATACTTTTATTTTCTTTTTCTACCGGCAAGCGGTTGGCACTATCTTCTTACTTCCCCTCACCATTTATTTCTCAAGAAATGAAATGGCAAGTTTATCAAAAGGAGATCTGTTGAAGATTTTCATGCAGGCCTTCTTAGGGTTTACGTTTGGTTTTAATGCTTATGGATTAGGGGTTAAGTATACATCGGCCACCTTAGGAGCTGCTGCATTTAATTGTATTCCTGTCACCACGTTCTTCTTTGCTTTTATTTTAAGGGTGGAGAAAGTAAATATAAAGAAAGCAAGTGGAATGGCAAAGGTTGGAGGCATAATGTTGTGCATTACTGGTGTTGCGGTTATTACATTTTACAAAGGTCCTTATTTAAAACCACTCAACAACGTTCACCTTTTTCAAACCCAACAAGCTCATGTTTCTTCCAAAAAAGAATGGATTCTTGGCTGTTCCTTGCTCCTCCTTTCTTCCCTTGCAGTGGGTTTGTGGTTTGTTCTCCAGGTTTGGGTTTTGAGAACTTGTCCATCTCCACTTGTAGTAACATTTGGACAGACATTTTCAAGTAGCATTCAGTCCTTTGTTGTGGCTATTGCAATCGAAAGAAACCCATCTCAATGGAAGCTGGCTTGGAACATTTCCCTAGCTGCAATTCTTTATTGTGGAGTTTTTGTAGTTTCCATTGGAAACTATTTGTCAAGCTGGGTGGTTAAGAAAAAGGGTCCAGTTTTTCAAGCTGTTACTACACCCTTCAATCTCATTGTTACACTTATTGGTTCAGAATTTCTTTTAAAGGATGGAATTTGCTTGGGAAGCGGAATTGGTGCTATATTGCTAGTTTTGAGTCTTTATAGTGTTTTATGGGGAAAAAAGAAGGAAGCATGTTGTCGTGATGCATCAAACAACAACAGCATCACCAATTCTGTTCAAGTAGAAAGGGAAATATTAGATAACATTTAA

Protein sequence

MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEKVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLGSGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDNI*
Homology
BLAST of CSPI06G26780 vs. ExPASy Swiss-Prot
Match: Q9FGG3 (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.1e-74
Identity = 153/351 (43.59%), Postives = 209/351 (59.54%), Query Frame = 0

Query: 5   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 64
           M SKKPY    + Q I+  M L+SKA ++ GMNTF+F FYRQA  TIFL PL  +F R  
Sbjct: 3   MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKS 62

Query: 65  MASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVE 124
              LS    +KIFM +  G T   +  G+ + YTSATL AA    +P  TFF A +  +E
Sbjct: 63  APPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGME 122

Query: 125 KVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLK-PL------NNVHLFQTQQAHVS-S 184
           ++ +K   G AK+ GI +C+ GV ++  YKGP LK PL         H  +    HVS  
Sbjct: 123 RLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNPGHVSGG 182

Query: 185 KKEWILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERN 244
              W+ GC L++ S++  GLW VLQ  VL+  PS L  T      SSIQSFV+AIA+ER+
Sbjct: 183 STSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERD 242

Query: 245 PSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFL 304
            S WKL WN+ L A++YCG  V  +  YL SWV++K+GPVF ++ TP +L+ TL+ S  L
Sbjct: 243 ISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAIL 302

Query: 305 LKDGICLGSGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVERE 348
           L + I LGS +G +LL++ LY VLWGK +E         NS  + + +++E
Sbjct: 303 LCEIISLGSIVGGLLLIIGLYCVLWGKSRE-------EKNSGDDKIDLQKE 346

BLAST of CSPI06G26780 vs. ExPASy Swiss-Prot
Match: Q6NMB7 (WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 5.8e-56
Identity = 129/322 (40.06%), Postives = 180/322 (55.90%), Query Frame = 0

Query: 5   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 64
           M   K   A +  Q ++AGM LLSK + S G N F+F FYRQA   + L P   +   ++
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 65  MASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVE 124
            + LS   LLKIF  +  G T   N Y + ++ T+AT  AA  N IP  TF  A + R+E
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 125 KVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGC 184
            V +KK+ G+AKV G M+ + G  V  F KGP L  +N+ +        V S K  + G 
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSL--INHYNSSTIPNGTVPSTKNSVKGS 181

Query: 185 SLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAW 244
             +L ++    LW ++Q  V++  P+ L +   Q   S IQS V A+A+ RNPS WK+ +
Sbjct: 182 ITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEF 241

Query: 245 NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLG 304
            + L ++ YCG+ V  +  +L  W ++KKGPVF A+ TP  LI+T I S FL K+   LG
Sbjct: 242 GLPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLG 301

Query: 305 SGIGAILLVLSLYSVLWGKKKE 327
           S  GA+LLV  LY  LWGK KE
Sbjct: 302 SVGGAVLLVCGLYLGLWGKTKE 321

BLAST of CSPI06G26780 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 7.1e-54
Identity = 120/356 (33.71%), Postives = 196/356 (55.06%), Query Frame = 0

Query: 2   EQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFS 61
           E  + S KPYFA +  Q  +AGM++++K S ++GM+ ++   YR A+ T  + P   +F 
Sbjct: 10  ESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFE 69

Query: 62  RNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 121
           R     ++    +++F+   LG     N Y +G+KYTS T   A  N +P  TF  A + 
Sbjct: 70  RKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLF 129

Query: 122 RVEKVNIKKASGMAKVGGIMLCITGVAVITFYKGP--------YLKPLNNVHLFQTQQAH 181
           R+E +++KK    AK+ G ++ + G  ++T YKGP        Y+   ++ H   T   +
Sbjct: 130 RMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKN 189

Query: 182 VSSKKEWILGCSLLLLSSLAVGLWFVLQVWVLRT-CPSPLVVTFGQTFSSSIQSFVVAIA 241
            SS KE++ G  LL+ ++LA    FVLQ  +L+T     L +T    F  ++Q+  V   
Sbjct: 190 SSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFV 249

Query: 242 IERNPSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIG 301
           +E NPS W++ W+++L A  Y G+   SI  Y+   V+KK+GPVF    +P  +++  + 
Sbjct: 250 MEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVM 309

Query: 302 SEFLLKDGICLGSGIGAILLVLSLYSVLWGKKKE---ACCRDA--SNNNSITNSVQ 344
             F+L + I LG  IGA+L+V+ LY+VLWGK+KE     C  A   +N+ +T  V+
Sbjct: 310 GSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVE 365

BLAST of CSPI06G26780 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 6.1e-45
Identity = 110/320 (34.38%), Postives = 170/320 (53.12%), Query Frame = 0

Query: 9   KPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMASL 68
           KP+   +  Q  +AG+S+++K + + GM+  +   YR  V TIF+ P   +  R     +
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 69  SKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEKVNI 128
           +     KI +   L  T   N Y  G+KYTSAT  AA  N +P   F  A+I R+EKVN+
Sbjct: 67  TLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNV 126

Query: 129 KKASGMAKVGGIMLCITGVAVITFYKGPYLK-PLNNVHLFQTQQAHVSSKKEWILGCSLL 188
           KK    AK+ G ++ + G  ++T  KGP +  P  N H      ++   K++   G SL+
Sbjct: 127 KKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGASLI 186

Query: 189 LLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIER-NPSQWKLAWNI 248
            +  +    +  LQ   L++ P  L +T    F  SI+S +VA+ IER NPS W +  + 
Sbjct: 187 AIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDS 246

Query: 249 SLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLGSG 308
            L A +Y GV    IG Y+   ++K +GPVF     P ++++  I    +L + + LG  
Sbjct: 247 KLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRI 306

Query: 309 IGAILLVLSLYSVLWGKKKE 327
           +GAI++VL LYSVLWGK K+
Sbjct: 307 LGAIVIVLGLYSVLWGKSKD 326

BLAST of CSPI06G26780 vs. ExPASy Swiss-Prot
Match: Q9M0B8 (WAT1-related protein At4g30420 OS=Arabidopsis thaliana OX=3702 GN=At4g30420 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.7e-44
Identity = 104/344 (30.23%), Postives = 178/344 (51.74%), Query Frame = 0

Query: 13  AALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE----MASL 72
           A  + Q  +AG++L ++A+   G++  +F  YRQA  TIF+ P  +Y SR +    ++SL
Sbjct: 2   AMTMIQLCYAGVTLFARATLVHGLSPRVFILYRQAFATIFIFPF-LYLSRRKSKIAISSL 61

Query: 73  SKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEKVNI 132
                  IF+ + +G T   N Y  G+  TS+++G+A  N IP  TF  +F+   EK+N+
Sbjct: 62  DLKSFSLIFLVSLIGITINQNLYLEGLYLTSSSMGSAVGNIIPAITFLISFLAGYEKLNL 121

Query: 133 KKASGMAKVGGIMLCITGVAVITFYKGP-YLKPLNNVHLFQTQQAHVSSKKEWILGCSLL 192
           +   G+AK+ G +LC+ G   +T  +GP  L   + + + ++   H+  +  W++GC  L
Sbjct: 122 RDIRGLAKIAGTILCVAGAISMTLLRGPKILNSESALPIAKSVLGHLKDQNTWLIGCLFL 181

Query: 193 LLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAWNIS 252
             S+L    W +LQV +    P  L ++       +IQ  VV   +E++P+ W L     
Sbjct: 182 FSSTLCWSFWLILQVPISAYYPDNLSLSAWMCLFGTIQCAVVTFFLEKDPNAWILHSYSE 241

Query: 253 LAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLGSGI 312
            A  LY G+   ++   + +W + K+GPVF A+  P   ++  I +     + I  GS I
Sbjct: 242 FATCLYAGIGASALSFTVQAWAIAKRGPVFSALFNPLCTVIVTILAALFFHEEIYTGSLI 301

Query: 313 GAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDN 352
           G + ++L LY+VLWGK K+        +N   + V++  E   N
Sbjct: 302 GGLGVILGLYTVLWGKAKDVMMNQDQRDNDQKSEVKIHIEDSSN 344

BLAST of CSPI06G26780 vs. ExPASy TrEMBL
Match: A0A0A0KF97 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_6G485120 PE=3 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 5.8e-192
Identity = 348/352 (98.86%), Postives = 348/352 (98.86%), Query Frame = 0

Query: 1   MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYF 60
           MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYF
Sbjct: 1   MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYF 60

Query: 61  SRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120
           SRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI
Sbjct: 61  SRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120

Query: 121 LRVEKVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEW 180
           LRVEKVN KKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEW
Sbjct: 121 LRVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEW 180

Query: 181 ILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQW 240
           ILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQW
Sbjct: 181 ILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQW 240

Query: 241 KLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDG 300
           KLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFL K G
Sbjct: 241 KLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLFKYG 300

Query: 301 ICLGSGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDNI 353
           ICLGSGIGAILLVLSLYSVLWGKKKEACC DASNNNSITNSVQVEREILDNI
Sbjct: 301 ICLGSGIGAILLVLSLYSVLWGKKKEACCHDASNNNSITNSVQVEREILDNI 352

BLAST of CSPI06G26780 vs. ExPASy TrEMBL
Match: A0A1S3B374 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103485250 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.5e-155
Identity = 280/352 (79.55%), Postives = 309/352 (87.78%), Query Frame = 0

Query: 1   MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYF 60
           ME+K  SKKPYF AL+CQ  FAGMSL SKA+++SGMNT+IF FYRQA+GTI LLPLTIYF
Sbjct: 1   MEEKKGSKKPYFGALVCQITFAGMSLFSKAAFASGMNTYIFLFYRQALGTIVLLPLTIYF 60

Query: 61  SRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120
           +R EM SLSKGD++KIF+QA LGFT G N YGLGVKYTSATLGAAAFNC+PVTTFFFA I
Sbjct: 61  ARKEMGSLSKGDVMKIFVQAILGFTVGLNVYGLGVKYTSATLGAAAFNCVPVTTFFFALI 120

Query: 121 LRVEKVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEW 180
            R+EKVNI+KASGMAK+GG++LCI GVAV+ FYKGPYLKPL   HLFQTQQ+HVSSKKEW
Sbjct: 121 SRMEKVNIRKASGMAKIGGLILCIAGVAVLAFYKGPYLKPLFKFHLFQTQQSHVSSKKEW 180

Query: 181 ILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQW 240
           ILGC LL L+ L  GLW+VLQ WVLR+CPSPLV+TFGQTFSS+IQSF VAIAIERNPS+W
Sbjct: 181 ILGCFLLFLTCLTWGLWYVLQAWVLRSCPSPLVLTFGQTFSSAIQSFFVAIAIERNPSEW 240

Query: 241 KLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDG 300
           KLAWNISLAAILYCG FV+ +GNYLSSWVVKKKGPVFQAVTTPFNLI TLIGSEFLL DG
Sbjct: 241 KLAWNISLAAILYCGAFVIPVGNYLSSWVVKKKGPVFQAVTTPFNLIFTLIGSEFLLNDG 300

Query: 301 ICLGSGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDNI 353
           I LGS IGAILLVLSLYSVLWGKKKEA C D  NN   TNSV  ER++LDNI
Sbjct: 301 IGLGSIIGAILLVLSLYSVLWGKKKEASCLDTKNN---TNSVPTERKVLDNI 349

BLAST of CSPI06G26780 vs. ExPASy TrEMBL
Match: A0A5A7SHD2 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G00470 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 9.1e-153
Identity = 285/348 (81.90%), Postives = 304/348 (87.36%), Query Frame = 0

Query: 5   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 64
           MNSK PYFAAL+CQT FAGMSLLSKAS+SSGMNT+IFFFYRQ VGTIFLLPLTIYF+R+ 
Sbjct: 1   MNSKMPYFAALVCQTTFAGMSLLSKASFSSGMNTYIFFFYRQIVGTIFLLPLTIYFTRS- 60

Query: 65  MASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVE 124
                               TFG+NAYG+GVKYTSATLGAAAFNCIPVTTF FA ILR E
Sbjct: 61  --------------------TFGWNAYGVGVKYTSATLGAAAFNCIPVTTFLFALILRAE 120

Query: 125 KVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGC 184
           KVNI+KASG AKVGGIMLCI GVAVITFYKGPYLKP+ N H FQTQQ+HVSSKKEWILGC
Sbjct: 121 KVNIRKASGKAKVGGIMLCIVGVAVITFYKGPYLKPVFNFHFFQTQQSHVSSKKEWILGC 180

Query: 185 SLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAW 244
            LLLLSSLA GLWFVLQ WVLR+CPSPLVVTFGQTFSS+IQSFVVAIAIERNPSQWKLAW
Sbjct: 181 CLLLLSSLAAGLWFVLQAWVLRSCPSPLVVTFGQTFSSAIQSFVVAIAIERNPSQWKLAW 240

Query: 245 NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLG 304
           NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLI+TLIGSEFLLKDGI LG
Sbjct: 241 NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIITLIGSEFLLKDGISLG 300

Query: 305 SGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDNI 353
           S IGA+LLVLSLYS+LWGKKKEA C DASNNN   +SV +ER+ L+NI
Sbjct: 301 STIGALLLVLSLYSILWGKKKEASCHDASNNN--ISSVPMERKTLNNI 325

BLAST of CSPI06G26780 vs. ExPASy TrEMBL
Match: A0A0A0KHU9 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_6G485110 PE=3 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 4.2e-142
Identity = 260/329 (79.03%), Postives = 285/329 (86.63%), Query Frame = 0

Query: 24  MSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMASLSKGDLLKIFMQAFLG 83
           MSL SKA++ SGMNT+IF FYRQA G+I LLPLTIYF+R EM SLSK D++KIF+Q  LG
Sbjct: 1   MSLFSKAAFVSGMNTYIFLFYRQAFGSIVLLPLTIYFARKEMRSLSKADVMKIFLQGLLG 60

Query: 84  FTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEKVNIKKASGMAKVGGIMLC 143
           FT G N YGLGVKYTSATLGAAAFNC+PVTTFFFA + R+EKVNI+KASGMAKVGGI+LC
Sbjct: 61  FTIGLNVYGLGVKYTSATLGAAAFNCVPVTTFFFALLSRMEKVNIRKASGMAKVGGIILC 120

Query: 144 ITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGCSLLLLSSLAVGLWFVLQVW 203
           I GVAV+ FYKGPYLKPL N HLFQTQQ+HVSSKKEWILGC LL L+ L  GLW+VLQ W
Sbjct: 121 IAGVAVLAFYKGPYLKPLFNFHLFQTQQSHVSSKKEWILGCFLLFLTCLTWGLWYVLQAW 180

Query: 204 VLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAWNISLAAILYCGVFVVSIGN 263
           VLR+CPSPLV+TFGQTFSS+IQSFVVAIAIERNPS+WKL WNISLAAILYCG FV+ +GN
Sbjct: 181 VLRSCPSPLVLTFGQTFSSAIQSFVVAIAIERNPSEWKLGWNISLAAILYCGTFVIPVGN 240

Query: 264 YLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLGSGIGAILLVLSLYSVLWGK 323
           YLSSWVVKKKGPVFQAVTTPFNLI TLIGSEFLL DGI LGS IGAILLVLSLYSVLWGK
Sbjct: 241 YLSSWVVKKKGPVFQAVTTPFNLIFTLIGSEFLLNDGISLGSIIGAILLVLSLYSVLWGK 300

Query: 324 KKEACCRDASNNNSITNSVQVEREILDNI 353
           KKEA C D  NN   TN V  E + L+NI
Sbjct: 301 KKEASCFDTENN---TNVVPTETKTLNNI 326

BLAST of CSPI06G26780 vs. ExPASy TrEMBL
Match: A0A6J1IL99 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111478060 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.6e-131
Identity = 237/349 (67.91%), Postives = 284/349 (81.38%), Query Frame = 0

Query: 4   KMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRN 63
           +M+S KPY AA+LCQ  FAGMSLLSKA+++SGMN +IF FYRQAVGT+FLLP+TI+F R 
Sbjct: 2   RMDSNKPYLAAILCQIAFAGMSLLSKAAFASGMNVYIFLFYRQAVGTLFLLPITIFFKRQ 61

Query: 64  EMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRV 123
           E+A LS G + KIFMQA LGFT   N YGLGVKYTSATLGAAAFNC+PVTTFFFA I R+
Sbjct: 62  EIAGLSVGVVFKIFMQASLGFTLALNVYGLGVKYTSATLGAAAFNCLPVTTFFFALIFRM 121

Query: 124 EKVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILG 183
           E+V I+KASGMAKVGGI++CI GVAV+ FY+GPY+KP  + HLF+T ++HVSS K WI G
Sbjct: 122 EEVKIRKASGMAKVGGIIVCIAGVAVLAFYRGPYIKPFFHYHLFETHESHVSSPKTWIFG 181

Query: 184 CSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLA 243
           C LLL + ++ GLWFVLQ  +L+T  SPLV+T GQT SS++QSFVVAIA+ERN S+W+L 
Sbjct: 182 CFLLLFACISWGLWFVLQALILKTGSSPLVLTCGQTLSSALQSFVVAIAVERNTSEWQLG 241

Query: 244 WNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICL 303
           WNI L ++LYCG FV+ I N+LSSWV+KKKGPVFQAVTTP NLI TLIGS+ LL DG+ L
Sbjct: 242 WNIRLVSVLYCGTFVICIANFLSSWVIKKKGPVFQAVTTPLNLIFTLIGSQLLLNDGVSL 301

Query: 304 GSGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDNI 353
           GSGIGA+LLVLSLYSVLWGK+KE  C D    N    SV  E+E LD+I
Sbjct: 302 GSGIGAMLLVLSLYSVLWGKRKEMSCSDEETKNH--PSVPPEKETLDHI 348

BLAST of CSPI06G26780 vs. NCBI nr
Match: XP_031742542.1 (WAT1-related protein At5g64700 isoform X1 [Cucumis sativus] >XP_031742543.1 WAT1-related protein At5g64700 isoform X1 [Cucumis sativus] >XP_031742544.1 WAT1-related protein At5g64700 isoform X1 [Cucumis sativus])

HSP 1 Score: 679.9 bits (1753), Expect = 1.2e-191
Identity = 348/352 (98.86%), Postives = 348/352 (98.86%), Query Frame = 0

Query: 1   MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYF 60
           MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYF
Sbjct: 1   MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYF 60

Query: 61  SRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120
           SRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI
Sbjct: 61  SRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120

Query: 121 LRVEKVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEW 180
           LRVEKVN KKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEW
Sbjct: 121 LRVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEW 180

Query: 181 ILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQW 240
           ILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQW
Sbjct: 181 ILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQW 240

Query: 241 KLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDG 300
           KLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFL K G
Sbjct: 241 KLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLFKYG 300

Query: 301 ICLGSGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDNI 353
           ICLGSGIGAILLVLSLYSVLWGKKKEACC DASNNNSITNSVQVEREILDNI
Sbjct: 301 ICLGSGIGAILLVLSLYSVLWGKKKEACCHDASNNNSITNSVQVEREILDNI 352

BLAST of CSPI06G26780 vs. NCBI nr
Match: XP_008441006.1 (PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo])

HSP 1 Score: 558.9 bits (1439), Expect = 3.1e-155
Identity = 280/352 (79.55%), Postives = 309/352 (87.78%), Query Frame = 0

Query: 1   MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYF 60
           ME+K  SKKPYF AL+CQ  FAGMSL SKA+++SGMNT+IF FYRQA+GTI LLPLTIYF
Sbjct: 1   MEEKKGSKKPYFGALVCQITFAGMSLFSKAAFASGMNTYIFLFYRQALGTIVLLPLTIYF 60

Query: 61  SRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120
           +R EM SLSKGD++KIF+QA LGFT G N YGLGVKYTSATLGAAAFNC+PVTTFFFA I
Sbjct: 61  ARKEMGSLSKGDVMKIFVQAILGFTVGLNVYGLGVKYTSATLGAAAFNCVPVTTFFFALI 120

Query: 121 LRVEKVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEW 180
            R+EKVNI+KASGMAK+GG++LCI GVAV+ FYKGPYLKPL   HLFQTQQ+HVSSKKEW
Sbjct: 121 SRMEKVNIRKASGMAKIGGLILCIAGVAVLAFYKGPYLKPLFKFHLFQTQQSHVSSKKEW 180

Query: 181 ILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQW 240
           ILGC LL L+ L  GLW+VLQ WVLR+CPSPLV+TFGQTFSS+IQSF VAIAIERNPS+W
Sbjct: 181 ILGCFLLFLTCLTWGLWYVLQAWVLRSCPSPLVLTFGQTFSSAIQSFFVAIAIERNPSEW 240

Query: 241 KLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDG 300
           KLAWNISLAAILYCG FV+ +GNYLSSWVVKKKGPVFQAVTTPFNLI TLIGSEFLL DG
Sbjct: 241 KLAWNISLAAILYCGAFVIPVGNYLSSWVVKKKGPVFQAVTTPFNLIFTLIGSEFLLNDG 300

Query: 301 ICLGSGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDNI 353
           I LGS IGAILLVLSLYSVLWGKKKEA C D  NN   TNSV  ER++LDNI
Sbjct: 301 IGLGSIIGAILLVLSLYSVLWGKKKEASCLDTKNN---TNSVPTERKVLDNI 349

BLAST of CSPI06G26780 vs. NCBI nr
Match: XP_031742545.1 (WAT1-related protein At5g64700 isoform X2 [Cucumis sativus] >KGN48679.2 hypothetical protein Csa_003266 [Cucumis sativus])

HSP 1 Score: 557.8 bits (1436), Expect = 6.9e-155
Identity = 284/288 (98.61%), Postives = 284/288 (98.61%), Query Frame = 0

Query: 65  MASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVE 124
           MASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVE
Sbjct: 1   MASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVE 60

Query: 125 KVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGC 184
           KVN KKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGC
Sbjct: 61  KVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGC 120

Query: 185 SLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAW 244
           SLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAW
Sbjct: 121 SLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAW 180

Query: 245 NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLG 304
           NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFL K GICLG
Sbjct: 181 NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLFKYGICLG 240

Query: 305 SGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDNI 353
           SGIGAILLVLSLYSVLWGKKKEACC DASNNNSITNSVQVEREILDNI
Sbjct: 241 SGIGAILLVLSLYSVLWGKKKEACCHDASNNNSITNSVQVEREILDNI 288

BLAST of CSPI06G26780 vs. NCBI nr
Match: KAA0025558.1 (WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 549.7 bits (1415), Expect = 1.9e-152
Identity = 285/348 (81.90%), Postives = 304/348 (87.36%), Query Frame = 0

Query: 5   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 64
           MNSK PYFAAL+CQT FAGMSLLSKAS+SSGMNT+IFFFYRQ VGTIFLLPLTIYF+R+ 
Sbjct: 1   MNSKMPYFAALVCQTTFAGMSLLSKASFSSGMNTYIFFFYRQIVGTIFLLPLTIYFTRS- 60

Query: 65  MASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVE 124
                               TFG+NAYG+GVKYTSATLGAAAFNCIPVTTF FA ILR E
Sbjct: 61  --------------------TFGWNAYGVGVKYTSATLGAAAFNCIPVTTFLFALILRAE 120

Query: 125 KVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGC 184
           KVNI+KASG AKVGGIMLCI GVAVITFYKGPYLKP+ N H FQTQQ+HVSSKKEWILGC
Sbjct: 121 KVNIRKASGKAKVGGIMLCIVGVAVITFYKGPYLKPVFNFHFFQTQQSHVSSKKEWILGC 180

Query: 185 SLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAW 244
            LLLLSSLA GLWFVLQ WVLR+CPSPLVVTFGQTFSS+IQSFVVAIAIERNPSQWKLAW
Sbjct: 181 CLLLLSSLAAGLWFVLQAWVLRSCPSPLVVTFGQTFSSAIQSFVVAIAIERNPSQWKLAW 240

Query: 245 NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLG 304
           NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLI+TLIGSEFLLKDGI LG
Sbjct: 241 NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIITLIGSEFLLKDGISLG 300

Query: 305 SGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDNI 353
           S IGA+LLVLSLYS+LWGKKKEA C DASNNN   +SV +ER+ L+NI
Sbjct: 301 STIGALLLVLSLYSILWGKKKEASCHDASNNN--ISSVPMERKTLNNI 325

BLAST of CSPI06G26780 vs. NCBI nr
Match: XP_004148640.1 (WAT1-related protein At5g64700 [Cucumis sativus])

HSP 1 Score: 549.7 bits (1415), Expect = 1.9e-152
Identity = 277/352 (78.69%), Postives = 305/352 (86.65%), Query Frame = 0

Query: 1   MEQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYF 60
           ME+KM+SKKPYF AL+CQ  FAGMSL SKA++ SGMNT+IF FYRQA G+I LLPLTIYF
Sbjct: 6   MEKKMDSKKPYFGALVCQLTFAGMSLFSKAAFVSGMNTYIFLFYRQAFGSIVLLPLTIYF 65

Query: 61  SRNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120
           +R EM SLSK D++KIF+Q  LGFT G N YGLGVKYTSATLGAAAFNC+PVTTFFFA +
Sbjct: 66  ARKEMRSLSKADVMKIFLQGLLGFTIGLNVYGLGVKYTSATLGAAAFNCVPVTTFFFALL 125

Query: 121 LRVEKVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEW 180
            R+EKVNI+KASGMAKVGGI+LCI GVAV+ FYKGPYLKPL N HLFQTQQ+HVSSKKEW
Sbjct: 126 SRMEKVNIRKASGMAKVGGIILCIAGVAVLAFYKGPYLKPLFNFHLFQTQQSHVSSKKEW 185

Query: 181 ILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQW 240
           ILGC LL L+ L  GLW+VLQ WVLR+CPSPLV+TFGQTFSS+IQSFVVAIAIERNPS+W
Sbjct: 186 ILGCFLLFLTCLTWGLWYVLQAWVLRSCPSPLVLTFGQTFSSAIQSFVVAIAIERNPSEW 245

Query: 241 KLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDG 300
           KL WNISLAAILYCG FV+ +GNYLSSWVVKKKGPVFQAVTTPFNLI TLIGSEFLL DG
Sbjct: 246 KLGWNISLAAILYCGTFVIPVGNYLSSWVVKKKGPVFQAVTTPFNLIFTLIGSEFLLNDG 305

Query: 301 ICLGSGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDNI 353
           I LGS IGAILLVLSLYSVLWGKKKEA C D  NN   TN V  E + L+NI
Sbjct: 306 ISLGSIIGAILLVLSLYSVLWGKKKEASCFDTENN---TNVVPTETKTLNNI 354

BLAST of CSPI06G26780 vs. TAIR 10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-75
Identity = 153/351 (43.59%), Postives = 209/351 (59.54%), Query Frame = 0

Query: 5   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 64
           M SKKPY    + Q I+  M L+SKA ++ GMNTF+F FYRQA  TIFL PL  +F R  
Sbjct: 3   MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKS 62

Query: 65  MASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVE 124
              LS    +KIFM +  G T   +  G+ + YTSATL AA    +P  TFF A +  +E
Sbjct: 63  APPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGME 122

Query: 125 KVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLK-PL------NNVHLFQTQQAHVS-S 184
           ++ +K   G AK+ GI +C+ GV ++  YKGP LK PL         H  +    HVS  
Sbjct: 123 RLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNPGHVSGG 182

Query: 185 KKEWILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERN 244
              W+ GC L++ S++  GLW VLQ  VL+  PS L  T      SSIQSFV+AIA+ER+
Sbjct: 183 STSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERD 242

Query: 245 PSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFL 304
            S WKL WN+ L A++YCG  V  +  YL SWV++K+GPVF ++ TP +L+ TL+ S  L
Sbjct: 243 ISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAIL 302

Query: 305 LKDGICLGSGIGAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVERE 348
           L + I LGS +G +LL++ LY VLWGK +E         NS  + + +++E
Sbjct: 303 LCEIISLGSIVGGLLLIIGLYCVLWGKSRE-------EKNSGDDKIDLQKE 346

BLAST of CSPI06G26780 vs. TAIR 10
Match: AT1G43650.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 219.5 bits (558), Expect = 4.1e-57
Identity = 129/322 (40.06%), Postives = 180/322 (55.90%), Query Frame = 0

Query: 5   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 64
           M   K   A +  Q ++AGM LLSK + S G N F+F FYRQA   + L P   +   ++
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 65  MASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVE 124
            + LS   LLKIF  +  G T   N Y + ++ T+AT  AA  N IP  TF  A + R+E
Sbjct: 62  SSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLE 121

Query: 125 KVNIKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGC 184
            V +KK+ G+AKV G M+ + G  V  F KGP L  +N+ +        V S K  + G 
Sbjct: 122 TVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSL--INHYNSSTIPNGTVPSTKNSVKGS 181

Query: 185 SLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAW 244
             +L ++    LW ++Q  V++  P+ L +   Q   S IQS V A+A+ RNPS WK+ +
Sbjct: 182 ITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEF 241

Query: 245 NISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLG 304
            + L ++ YCG+ V  +  +L  W ++KKGPVF A+ TP  LI+T I S FL K+   LG
Sbjct: 242 GLPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLG 301

Query: 305 SGIGAILLVLSLYSVLWGKKKE 327
           S  GA+LLV  LY  LWGK KE
Sbjct: 302 SVGGAVLLVCGLYLGLWGKTKE 321

BLAST of CSPI06G26780 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 212.6 bits (540), Expect = 5.1e-55
Identity = 120/356 (33.71%), Postives = 196/356 (55.06%), Query Frame = 0

Query: 2   EQKMNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFS 61
           E  + S KPYFA +  Q  +AGM++++K S ++GM+ ++   YR A+ T  + P   +F 
Sbjct: 10  ESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFE 69

Query: 62  RNEMASLSKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 121
           R     ++    +++F+   LG     N Y +G+KYTS T   A  N +P  TF  A + 
Sbjct: 70  RKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLF 129

Query: 122 RVEKVNIKKASGMAKVGGIMLCITGVAVITFYKGP--------YLKPLNNVHLFQTQQAH 181
           R+E +++KK    AK+ G ++ + G  ++T YKGP        Y+   ++ H   T   +
Sbjct: 130 RMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKN 189

Query: 182 VSSKKEWILGCSLLLLSSLAVGLWFVLQVWVLRT-CPSPLVVTFGQTFSSSIQSFVVAIA 241
            SS KE++ G  LL+ ++LA    FVLQ  +L+T     L +T    F  ++Q+  V   
Sbjct: 190 SSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFV 249

Query: 242 IERNPSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIG 301
           +E NPS W++ W+++L A  Y G+   SI  Y+   V+KK+GPVF    +P  +++  + 
Sbjct: 250 MEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVM 309

Query: 302 SEFLLKDGICLGSGIGAILLVLSLYSVLWGKKKE---ACCRDA--SNNNSITNSVQ 344
             F+L + I LG  IGA+L+V+ LY+VLWGK+KE     C  A   +N+ +T  V+
Sbjct: 310 GSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVE 365

BLAST of CSPI06G26780 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 183.0 bits (463), Expect = 4.3e-46
Identity = 110/320 (34.38%), Postives = 170/320 (53.12%), Query Frame = 0

Query: 9   KPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMASL 68
           KP+   +  Q  +AG+S+++K + + GM+  +   YR  V TIF+ P   +  R     +
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 69  SKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEKVNI 128
           +     KI +   L  T   N Y  G+KYTSAT  AA  N +P   F  A+I R+EKVN+
Sbjct: 67  TLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNV 126

Query: 129 KKASGMAKVGGIMLCITGVAVITFYKGPYLK-PLNNVHLFQTQQAHVSSKKEWILGCSLL 188
           KK    AK+ G ++ + G  ++T  KGP +  P  N H      ++   K++   G SL+
Sbjct: 127 KKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGASLI 186

Query: 189 LLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIER-NPSQWKLAWNI 248
            +  +    +  LQ   L++ P  L +T    F  SI+S +VA+ IER NPS W +  + 
Sbjct: 187 AIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDS 246

Query: 249 SLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLGSG 308
            L A +Y GV    IG Y+   ++K +GPVF     P ++++  I    +L + + LG  
Sbjct: 247 KLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRI 306

Query: 309 IGAILLVLSLYSVLWGKKKE 327
           +GAI++VL LYSVLWGK K+
Sbjct: 307 LGAIVIVLGLYSVLWGKSKD 326

BLAST of CSPI06G26780 vs. TAIR 10
Match: AT4G30420.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 179.5 bits (454), Expect = 4.8e-45
Identity = 104/344 (30.23%), Postives = 178/344 (51.74%), Query Frame = 0

Query: 13  AALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE----MASL 72
           A  + Q  +AG++L ++A+   G++  +F  YRQA  TIF+ P  +Y SR +    ++SL
Sbjct: 2   AMTMIQLCYAGVTLFARATLVHGLSPRVFILYRQAFATIFIFPF-LYLSRRKSKIAISSL 61

Query: 73  SKGDLLKIFMQAFLGFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEKVNI 132
                  IF+ + +G T   N Y  G+  TS+++G+A  N IP  TF  +F+   EK+N+
Sbjct: 62  DLKSFSLIFLVSLIGITINQNLYLEGLYLTSSSMGSAVGNIIPAITFLISFLAGYEKLNL 121

Query: 133 KKASGMAKVGGIMLCITGVAVITFYKGP-YLKPLNNVHLFQTQQAHVSSKKEWILGCSLL 192
           +   G+AK+ G +LC+ G   +T  +GP  L   + + + ++   H+  +  W++GC  L
Sbjct: 122 RDIRGLAKIAGTILCVAGAISMTLLRGPKILNSESALPIAKSVLGHLKDQNTWLIGCLFL 181

Query: 193 LLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAWNIS 252
             S+L    W +LQV +    P  L ++       +IQ  VV   +E++P+ W L     
Sbjct: 182 FSSTLCWSFWLILQVPISAYYPDNLSLSAWMCLFGTIQCAVVTFFLEKDPNAWILHSYSE 241

Query: 253 LAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLLKDGICLGSGI 312
            A  LY G+   ++   + +W + K+GPVF A+  P   ++  I +     + I  GS I
Sbjct: 242 FATCLYAGIGASALSFTVQAWAIAKRGPVFSALFNPLCTVIVTILAALFFHEEIYTGSLI 301

Query: 313 GAILLVLSLYSVLWGKKKEACCRDASNNNSITNSVQVEREILDN 352
           G + ++L LY+VLWGK K+        +N   + V++  E   N
Sbjct: 302 GGLGVILGLYTVLWGKAKDVMMNQDQRDNDQKSEVKIHIEDSSN 344

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FGG32.1e-7443.59WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2... [more]
Q6NMB75.8e-5640.06WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2... [more]
Q9FL417.1e-5433.71WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
O806386.1e-4534.38WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2... [more]
Q9M0B86.7e-4430.23WAT1-related protein At4g30420 OS=Arabidopsis thaliana OX=3702 GN=At4g30420 PE=3... [more]
Match NameE-valueIdentityDescription
A0A0A0KF975.8e-19298.86WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_6G485120 PE=3 SV=1[more]
A0A1S3B3741.5e-15579.55WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103485250 PE=3 SV=1[more]
A0A5A7SHD29.1e-15381.90WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253... [more]
A0A0A0KHU94.2e-14279.03WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_6G485110 PE=3 SV=1[more]
A0A6J1IL992.6e-13167.91WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111478060 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_031742542.11.2e-19198.86WAT1-related protein At5g64700 isoform X1 [Cucumis sativus] >XP_031742543.1 WAT1... [more]
XP_008441006.13.1e-15579.55PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo][more]
XP_031742545.16.9e-15598.61WAT1-related protein At5g64700 isoform X2 [Cucumis sativus] >KGN48679.2 hypothet... [more]
KAA0025558.11.9e-15281.90WAT1-related protein [Cucumis melo var. makuwa][more]
XP_004148640.11.9e-15278.69WAT1-related protein At5g64700 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT5G64700.11.5e-7543.59nodulin MtN21 /EamA-like transporter family protein [more]
AT1G43650.14.1e-5740.06nodulin MtN21 /EamA-like transporter family protein [more]
AT5G07050.15.1e-5533.71nodulin MtN21 /EamA-like transporter family protein [more]
AT2G39510.14.3e-4634.38nodulin MtN21 /EamA-like transporter family protein [more]
AT4G30420.14.8e-4530.23nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 182..320
e-value: 5.0E-8
score: 33.2
coord: 9..151
e-value: 1.9E-14
score: 54.0
NoneNo IPR availablePANTHERPTHR31218:SF166WAT1-RELATED PROTEINcoord: 9..335
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 44..154
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 9..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G26780.1CSPI06G26780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity