CSPI06G26360 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G26360
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial
LocationChr6: 23291156 .. 23295429 (+)
RNA-Seq ExpressionCSPI06G26360
SyntenyCSPI06G26360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTCTCTCTCTCTCTTTTTCTTCAACGGATAGTGCTCATACGATGGCTTCTTATCCGATTGGCTGTGGAGTCTGAAATTAGATAATCATTTCTTTAATGGAATTTAAGGGACTACGTCAGATTGCAACAAACTTACCAAAATCCCTCCTTTCCTTCATTTATCTTACGAACCCCAATAAATTTATGCTATATTCTCCCAAGTAATTCATCAATCTAGAGCACTCTCCGTTCAGAAATGGCTTTTCAGGTGAGAAAAGCAACAACGAAGATCATTCCCCATTTTAGAAACGGGATGGGTTTCTTCTTGGGTCCTCTCGAAATGGGTTTCTTCAACTCTTATTCAACCCATCTTCGTAATACTTCATTTTCTGCTCATAAGATCGGAACCAGAATTTTGGGTTCTGAAAATATGCCTACCCATTTGTTTTCTCGTCGTTTTGAATCGATTAAAGTGGGAAAGCAGTTGAATTGTGTCAACGATGATCGTGACGATCAGGTAGAATACACTATGATATGGTCAACTTTGATTAGTTTCTTCCAATTTCATATTTCATATTCTTTGTTTTTATAACCCCATATCGTTTTAGATAAGATACCTCTTAGTTTTTTAATCTTTATGTTGACGAGTGAGATTGTGTTCTTGTGATGACTGCATCTCTATAATTATTTTTCTCAAAATGGGATCTGATTGGTTGATAATGTAGCTTTAGACTTTGAACTGTATTTTTTTTTTCTTTTCATTCTGCTTGTCAACTCCCTCCTCTTTAGTGTATGTTAGACTTTGAGACATTTCTATTTGAACTGAGAGAGATATTAATAAGTAAAACTTTGAGGGAAATTTCTGTTTTTAGAGTGCGCCACCCCCCGAGTTACATGTCAATATGGTTGTTCTTCCACTTGTGTAGTGTGGAAGGTGGAAAATGTATGCCAAAAAAGAGCAGAAAAAGAGATTACTTTGTTCATTGAGATAATGTATTTTGGAAAATATGCATTTCAAAAAGAGGAGCCAAGCAACCAATAGCAAAAAGGTGCTCCAATCCAGTAAAATAAGGTCTAGAAGGTTAATCGTGAAAAGACCTCTTCACCGATGCGCAAAGAGAAAGATAGAATCTATCAAGGTCAAACATCGCTCTGCTATTTCTCCTGCCATGCTCAAATAAAGACATCCTCTCAAAGTCTTATCTTAAATTTCTCTGTTCTTACCAATTGTGAACAAGTTCATGATATTCAACAATATTCCATTATGTGCAAGGTTTTGGAGTTCCCAGGAGGAAAGGTAAAATTTACTTCTCAACTGAGTTTCATCCCTGATCGCGAGGAGGAGCGGATACCTTGCTTTCGGGTTCTTGATGACAACGGCCGTCCTTGTATGTACAGTAATTTTACACAGGTAATTGTATGCTGAAAATGCCCCAACTCCAAGTGCAACGATAGTTATTTATGTAGTTCCTTTCATTTAGGTAAGCAAGGAGGTCGCTATGAAGATATACCATGACATGGTTACCCTCCAAACAATGGACACGATCTTCTACGAAGCGCAAAGGCAAGGAAGAATTTCCTTTTATGTGACCTCCCTTGGAGAAGAAGCAATCAACGTTGCTTCTGCCGCAGCACTGTCATTGGATGACCTCATTGTTCCACAGGTTACTTCATATCTCCACCTCGTCATCCTCCTTAGTAATAGGAGTACATTCTCATCCTCACATGGAAAGAACACTCTTTGCTTACACTAACATCTTATCTAATTAGTTCACATTTGTGAGATTGGTAATTCTTAGTGTAATTCTCCCATGTCATACAGTACAGGGAGGCAGGAGTGCTTATATGGCGTGGTTTCACTCTGAAAGAATTTGCAAGCCAGTGCTTTGGAAACAAATATGATTATTGTAAAGGGAGGCAGATGCCTGTCCATTATGGGTCTAAGAGACACAACTACTTCACTGTAGCATCAACAATTGCGTAAGATTTGAGCTCCATATGTCAAAACTTTTAACCAGATATTTTAACACTTCGAATCTTATTACTCGTATTTTACTTCAATTTCTAGTTCTCAAATTCCTCATGCTGTTGGTGCTGCATATTCAATGAAGATGGATAAGAAAGATGCTTGTGCAGTCACTTATTTTGGCGATGGAGGAACAAGTGAGGTACTGAATACTATGAAAAAATCGTAGTTAGAATGTGTTGCATCTAACATTACCTTTGTACAGAACACTATGAAAAACTCTTATTTGGGTTGCTTGTTGATATTTACTCTATCGGTGTTCGTAGTTTTTATATTTGATGCTTTGCGCTTCTTCTCCTTTTTCATTTTCTTGTCATTCTTTGGGACTAATGTAACTTGCTCGTTAGTCTCGTTTCATTTCATCAATGAAAAAACTTTGTTAACCTTACCTCTGTAGAATTTTTAGGAGATAAGCAAATAGAAAGTCATTCTACTAAATCTGGTTGAGTCTATGGAGTAATATTGTGTACTCTGGCTTGTTACAAGGTTGATTTTTCTGTTGTTTCTTTTATCATGTTACATTAGGGAGATTTCCACGCAGCTTTAAATTTTGCCGCAGTTTTGGAGGCACCTGTTATCTTTATTTGCCGGAACAATGGATGGGCGATCAGTACTCCTATTTCAGACCAGTTTCGAAGTATCTCATTCTTCTCAGTATTTCCAGTTGTGAAAAATGTATTATGAAATCTAGATTTTGAAGTGTTCAATTATTGTGCAGGTGACGGGGTGGTTGTAAAAGGCCAGGCTTATGGAGTTCGCAGTATCCGTGTAGATGGCAATGATGCACTTGCTGTATATAATGCAGTTCGAGCTGCTCGAGAAATGGCAATTCAAGAACAGAGGCCAATTTTAATTGAGGTAACAGGTGTACACAAACTCTCTCTCTTAATATGGATACCTTTGATGATCTGGTGTCTTTTGTTTTCATTAACAGGCTCTCACTTATAGAGCTGGACACCACACGACATCAGATGATTCGACCAGGTATCGTCCAGCTGAGGAAATGGAATGGTGGAGAGTTGCAATGGACCCTGTCTCTAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTAATGAGGCTGAAGCAGAGCTTAGAAGTCGCTTAAGAAAACAGGTATATTTTGTGTTTAGAATAAAGTCTTTTCAACGCCGCACACACACACCTACGTGCACATGCGCGCACACACGAGAGACCACTTCAATCTATGAACAAAAAATTGAAAAACAAGAACAAAAAGGACTAACAAGCGAAGGGAGTTGACCAGAAAGGTTACAAAAACACCATCCAGTGTGAATGGTGGTCAGCGAGGTTAAACACCATACACGAGAGACCACTTCAATCTATGAAAAAAGAATTGAAAAACAAGAACAAAAAGGACAATAAACAAGCAAAGGGAGTTGATCAGAAAAGGTTACAAAAACACCATCCAGTGTCAATGGTGGTCAGCGAGGTTAAACACCATACACCATCCAATGTCAATGGTGAAATTTGAGTTGAGTTGGCCAGAAACATAGTAAATGAGATAAACAAGAATAGGATATATTCAACTCAAAATTATCATTTGCATTTAGGCTTCTTGAAGAAGAAAGACCTTTTTTTTTTCTCTTTAAAAAACCGTTTTTTCAAGACAACAAAAACATATTTTTGAGAGTTAAGTCTTTTTAAGATTTTACTGGAGAATGATCACAAATAACACAAAAAAAAATGAAACTATTTATAAAATATGGTAAAATTTTCAAATGGATTAGAGAGGGTTAGATGAATTTTAATATATTTTGTAAATAGTTTCAATATTGTGCTATTCTTTGAAAATGTCCCACTCTAATACTCAATTAAAATTTTGCAAATACTTTAAAAAGTATGTTTAAAAAAAAGAAACGGTGAGTGAACAAAGTGATATATTTTGAAAGTACTTTCATGTCGACCAAATTCTTGTCTATCATGTACGTTTTTAATTCCATGTGTTTTTTGGTTCCAACAGCTATTAGAGGAAATCCAGTTGGCAGAGAGATCTGAGAAGCCCCCTGTGGCAGATGCTTTCACAGATGTGTATGATGTTCCTCCTTCCAACCTCCAAGAGCAAGAGAAGTGGTTAAGAAAAACAATCAAAACACACCCACAAGATTATCCATCTAAATTTCCTCTGTAATTTGTCATTGGGTTTTGAAACACTCTGCCTTAGAAATATGAGAATGGCGGTGCCATTGTTTATGAATAAAGGCCATAAGGCATATTGATCTAATAATTAAGCAAGTGTTTCAC

mRNA sequence

GCTCTCTCTCTCTCTTTTTCTTCAACGGATAGTGCTCATACGATGGCTTCTTATCCGATTGGCTGTGGAGTCTGAAATTAGATAATCATTTCTTTAATGGAATTTAAGGGACTACGTCAGATTGCAACAAACTTACCAAAATCCCTCCTTTCCTTCATTTATCTTACGAACCCCAATAAATTTATGCTATATTCTCCCAAGTAATTCATCAATCTAGAGCACTCTCCGTTCAGAAATGGCTTTTCAGGTGAGAAAAGCAACAACGAAGATCATTCCCCATTTTAGAAACGGGATGGGTTTCTTCTTGGGTCCTCTCGAAATGGGTTTCTTCAACTCTTATTCAACCCATCTTCGTAATACTTCATTTTCTGCTCATAAGATCGGAACCAGAATTTTGGGTTCTGAAAATATGCCTACCCATTTGTTTTCTCGTCGTTTTGAATCGATTAAAGTGGGAAAGCAGTTGAATTGTGTCAACGATGATCGTGACGATCAGGTTTTGGAGTTCCCAGGAGGAAAGGTAAAATTTACTTCTCAACTGAGTTTCATCCCTGATCGCGAGGAGGAGCGGATACCTTGCTTTCGGGTTCTTGATGACAACGGCCGTCCTTGTATGTACAGTAATTTTACACAGGTAAGCAAGGAGGTCGCTATGAAGATATACCATGACATGGTTACCCTCCAAACAATGGACACGATCTTCTACGAAGCGCAAAGGCAAGGAAGAATTTCCTTTTATGTGACCTCCCTTGGAGAAGAAGCAATCAACGTTGCTTCTGCCGCAGCACTGTCATTGGATGACCTCATTGTTCCACAGTACAGGGAGGCAGGAGTGCTTATATGGCGTGGTTTCACTCTGAAAGAATTTGCAAGCCAGTGCTTTGGAAACAAATATGATTATTGTAAAGGGAGGCAGATGCCTGTCCATTATGGGTCTAAGAGACACAACTACTTCACTGTAGCATCAACAATTGCTTCTCAAATTCCTCATGCTGTTGGTGCTGCATATTCAATGAAGATGGATAAGAAAGATGCTTGTGCAGTCACTTATTTTGGCGATGGAGGAACAAGTGAGGGAGATTTCCACGCAGCTTTAAATTTTGCCGCAGTTTTGGAGGCACCTGTTATCTTTATTTGCCGGAACAATGGATGGGCGATCAGTACTCCTATTTCAGACCAGTTTCGAAGTGACGGGGTGGTTGTAAAAGGCCAGGCTTATGGAGTTCGCAGTATCCGTGTAGATGGCAATGATGCACTTGCTGTATATAATGCAGTTCGAGCTGCTCGAGAAATGGCAATTCAAGAACAGAGGCCAATTTTAATTGAGGCTCTCACTTATAGAGCTGGACACCACACGACATCAGATGATTCGACCAGGTATCGTCCAGCTGAGGAAATGGAATGGTGGAGAGTTGCAATGGACCCTGTCTCTAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTAATGAGGCTGAAGCAGAGCTTAGAAGTCGCTTAAGAAAACAGCTATTAGAGGAAATCCAGTTGGCAGAGAGATCTGAGAAGCCCCCTGTGGCAGATGCTTTCACAGATGTGTATGATGTTCCTCCTTCCAACCTCCAAGAGCAAGAGAAGTGGTTAAGAAAAACAATCAAAACACACCCACAAGATTATCCATCTAAATTTCCTCTGTAATTTGTCATTGGGTTTTGAAACACTCTGCCTTAGAAATATGAGAATGGCGGTGCCATTGTTTATGAATAAAGGCCATAAGGCATATTGATCTAATAATTAAGCAAGTGTTTCAC

Coding sequence (CDS)

ATGGCTTTTCAGGTGAGAAAAGCAACAACGAAGATCATTCCCCATTTTAGAAACGGGATGGGTTTCTTCTTGGGTCCTCTCGAAATGGGTTTCTTCAACTCTTATTCAACCCATCTTCGTAATACTTCATTTTCTGCTCATAAGATCGGAACCAGAATTTTGGGTTCTGAAAATATGCCTACCCATTTGTTTTCTCGTCGTTTTGAATCGATTAAAGTGGGAAAGCAGTTGAATTGTGTCAACGATGATCGTGACGATCAGGTTTTGGAGTTCCCAGGAGGAAAGGTAAAATTTACTTCTCAACTGAGTTTCATCCCTGATCGCGAGGAGGAGCGGATACCTTGCTTTCGGGTTCTTGATGACAACGGCCGTCCTTGTATGTACAGTAATTTTACACAGGTAAGCAAGGAGGTCGCTATGAAGATATACCATGACATGGTTACCCTCCAAACAATGGACACGATCTTCTACGAAGCGCAAAGGCAAGGAAGAATTTCCTTTTATGTGACCTCCCTTGGAGAAGAAGCAATCAACGTTGCTTCTGCCGCAGCACTGTCATTGGATGACCTCATTGTTCCACAGTACAGGGAGGCAGGAGTGCTTATATGGCGTGGTTTCACTCTGAAAGAATTTGCAAGCCAGTGCTTTGGAAACAAATATGATTATTGTAAAGGGAGGCAGATGCCTGTCCATTATGGGTCTAAGAGACACAACTACTTCACTGTAGCATCAACAATTGCTTCTCAAATTCCTCATGCTGTTGGTGCTGCATATTCAATGAAGATGGATAAGAAAGATGCTTGTGCAGTCACTTATTTTGGCGATGGAGGAACAAGTGAGGGAGATTTCCACGCAGCTTTAAATTTTGCCGCAGTTTTGGAGGCACCTGTTATCTTTATTTGCCGGAACAATGGATGGGCGATCAGTACTCCTATTTCAGACCAGTTTCGAAGTGACGGGGTGGTTGTAAAAGGCCAGGCTTATGGAGTTCGCAGTATCCGTGTAGATGGCAATGATGCACTTGCTGTATATAATGCAGTTCGAGCTGCTCGAGAAATGGCAATTCAAGAACAGAGGCCAATTTTAATTGAGGCTCTCACTTATAGAGCTGGACACCACACGACATCAGATGATTCGACCAGGTATCGTCCAGCTGAGGAAATGGAATGGTGGAGAGTTGCAATGGACCCTGTCTCTAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTAATGAGGCTGAAGCAGAGCTTAGAAGTCGCTTAAGAAAACAGCTATTAGAGGAAATCCAGTTGGCAGAGAGATCTGAGAAGCCCCCTGTGGCAGATGCTTTCACAGATGTGTATGATGTTCCTCCTTCCAACCTCCAAGAGCAAGAGAAGTGGTTAAGAAAAACAATCAAAACACACCCACAAGATTATCCATCTAAATTTCCTCTGTAA

Protein sequence

MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMPTHLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFPL*
Homology
BLAST of CSPI06G26360 vs. ExPASy Swiss-Prot
Match: Q84JL2 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g09300 PE=1 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 3.7e-178
Identity = 292/395 (73.92%), Postives = 351/395 (88.86%), Query Frame = 0

Query: 87  QVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGRPCMYSNFTQVSKEVAMKIYHDM 146
           QV++FPGGKV FT ++ FI + ++ER+PC+RVLDDNG+    S F QVS+EVA+KIY DM
Sbjct: 78  QVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDM 137

Query: 147 VTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRGF 206
           VTLQ MD IFYEAQRQGR+SFY T++GEEAIN+ASAAAL+  D+I PQYRE GVL+WRGF
Sbjct: 138 VTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGF 197

Query: 207 TLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKD 266
           TL+EFA+QCFGNK DY KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KMDKKD
Sbjct: 198 TLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDKKD 257

Query: 267 ACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVVVKGQ 326
           ACAVTYFGDGGTSEGDFHAALN AAV+EAPV+FICRNNGWAISTP SDQFRSDGVVVKG+
Sbjct: 258 ACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVKGR 317

Query: 327 AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPAE 386
           AYG+RSIRVDGNDALA+Y+AV  AREMAI+EQRPILIEALTYR GHH+TSDDSTRYR A 
Sbjct: 318 AYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAG 377

Query: 387 EMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLRKQLLEEIQLAERSEKPPVADAF 446
           E+EWW  A +P+SRFR WIESNGWWS++ E++LRSR++K++LE +++AE++EKP + + F
Sbjct: 378 EIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQNMF 437

Query: 447 TDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFPL 482
           +DVYDVPPSNL+EQE  +R+TI +HPQDYPS  PL
Sbjct: 438 SDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

BLAST of CSPI06G26360 vs. ExPASy Swiss-Prot
Match: Q9LPL5 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g21400 PE=2 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 3.8e-167
Identity = 272/398 (68.34%), Postives = 337/398 (84.67%), Query Frame = 0

Query: 82  DDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGRPCMYSNFTQVSKEVAMK 141
           D+ D Q L+FPGGKV +TS++ FIP+    RIPC+RVLD++GR    S+F  VS+++A++
Sbjct: 73  DEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVR 132

Query: 142 IYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVL 201
           +Y  M TLQ MD IFYEAQRQGRISFY+TS+GEEAIN+ASAAALS DD+++PQYRE GVL
Sbjct: 133 MYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVL 192

Query: 202 IWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMK 261
           +WRGFTL+EFA+QCFGNK DY KGRQMP+HYGS R NYFT++S IA+Q+P A G  YS+K
Sbjct: 193 LWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTISSPIATQLPQAAGVGYSLK 252

Query: 262 MDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGV 321
           MDKK+AC VT+ GDGGTSEGDFHA LNFAAV+EAPV+FICRNNGWAIST IS+QFRSDG+
Sbjct: 253 MDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHISEQFRSDGI 312

Query: 322 VVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTR 381
           VVKGQAYG+RSIRVDGNDALAVY+AVR+AREMA+ EQRP+LIE +TYR GHH+TSDDST+
Sbjct: 313 VVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPVLIEMMTYRVGHHSTSDDSTK 372

Query: 382 YRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLRKQLLEEIQLAERSEKPP 441
           YR A+E+++W+++ +PV+RFRKW+E NGWWS E E++LRS  RKQLL+ IQ AE+ EK P
Sbjct: 373 YRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSNARKQLLQAIQAAEKWEKQP 432

Query: 442 VADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKF 480
           + + F DVYDV P NL+EQE  L++ +K  PQDYP  F
Sbjct: 433 LTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYPPGF 470

BLAST of CSPI06G26360 vs. ExPASy Swiss-Prot
Match: A5A6H9 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes OX=9598 GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 7.3e-110
Identity = 193/391 (49.36%), Postives = 257/391 (65.73%), Query Frame = 0

Query: 86  DQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGRPCMYSNFTQVSKEVAMKIYHD 145
           D   +FPG   +F  +L FI       IP +RV+D  G+    S    + KE  +K+Y  
Sbjct: 49  DDKPQFPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKS 108

Query: 146 MVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRG 205
           M  L TMD I YE+QRQGRISFY+T+ GEE  +V SAAAL   DL+  QYREAGVL++R 
Sbjct: 109 MTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRD 168

Query: 206 FTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKK 265
           + L+ F +QC+GN  D  KGRQMPVHYG K  ++ T++S +A+QIP AVGAAY+ K    
Sbjct: 169 YPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANA 228

Query: 266 DACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVVVKG 325
           +   + YFG+G  SEGD HA  NFAA LE P+IF CRNNG+AISTP S+Q+R DG+  +G
Sbjct: 229 NRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARG 288

Query: 326 QAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPA 385
             YG+ SIRVDGND  AVYNA + AR  A+ E +P LIEA+TYR GHH+TSDDS+ YR  
Sbjct: 289 PGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSV 348

Query: 386 EEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLRKQLLEEIQLAERSEKPPVADA 445
           +E+ +W     PVSR R ++ S GWW  E E   R + RK+++E  + AER  KP     
Sbjct: 349 DEVNYWDKQDHPVSRLRHYLLSQGWWDEEQEKAWRKQSRKKVMEAFEQAERKPKPNPNLL 408

Query: 446 FTDVYDVPPSNLQEQEKWLRKTIKTHPQDYP 477
           F+DVY   P+ L++Q++ L + ++T+ + YP
Sbjct: 409 FSDVYQEMPAQLRKQQESLARHLQTYGEHYP 439

BLAST of CSPI06G26360 vs. ExPASy Swiss-Prot
Match: P50136 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus OX=10090 GN=Bckdha PE=1 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.6e-109
Identity = 190/391 (48.59%), Postives = 258/391 (65.98%), Query Frame = 0

Query: 86  DQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGRPCMYSNFTQVSKEVAMKIYHD 145
           D   +FPG   +F  +L FI       IP +RV+D  G+    S    + +E  +K Y  
Sbjct: 46  DDKPQFPGASAEFVDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEEVLKFYRS 105

Query: 146 MVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRG 205
           M  L TMD I YE+QR+GRISFY+T+ GEE  +V SAAAL   DL+  QYREAGVL++R 
Sbjct: 106 MTLLNTMDRILYESQREGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYRD 165

Query: 206 FTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKK 265
           + L+ F SQC+GN  D  KGRQMPVHYG K  ++ T++S +A+QIP AVGAAY+ K    
Sbjct: 166 YPLELFMSQCYGNVNDPGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANA 225

Query: 266 DACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVVVKG 325
           +   + YFG+G  SEGD HA  NFAA LE P+IF CRNNG+AISTP S+Q+R DG+  +G
Sbjct: 226 NRIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARG 285

Query: 326 QAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPA 385
             YG++SIRVDGND  AVYNA + AR  A+ E +P LIEA+TYR GHH+TSDDS+ YR  
Sbjct: 286 PGYGIKSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSV 345

Query: 386 EEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLRKQLLEEIQLAERSEKPPVADA 445
           +E+ +W     P+SR R+++ + GWW  E E   R + RK+++E  + AER  KP  +  
Sbjct: 346 DEVNYWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQAERKLKPNPSLL 405

Query: 446 FTDVYDVPPSNLQEQEKWLRKTIKTHPQDYP 477
           F+DVY   P+ L+ Q++ L + ++T+ + YP
Sbjct: 406 FSDVYQEMPAQLRRQQESLARHLQTYGEHYP 436

BLAST of CSPI06G26360 vs. ExPASy Swiss-Prot
Match: P12694 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Homo sapiens OX=9606 GN=BCKDHA PE=1 SV=2)

HSP 1 Score: 397.5 bits (1020), Expect = 2.1e-109
Identity = 191/391 (48.85%), Postives = 257/391 (65.73%), Query Frame = 0

Query: 86  DQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGRPCMYSNFTQVSKEVAMKIYHD 145
           D   +FPG   +F  +L FI       IP +RV+D  G+    S    + KE  +K+Y  
Sbjct: 49  DDKPQFPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKS 108

Query: 146 MVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRG 205
           M  L TMD I YE+QRQGRISFY+T+ GEE  +V SAAAL   DL+  QYREAGVL++R 
Sbjct: 109 MTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRD 168

Query: 206 FTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKK 265
           + L+ F +QC+GN  D  KGRQMPVHYG K  ++ T++S +A+QIP AVGAAY+ K    
Sbjct: 169 YPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANA 228

Query: 266 DACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVVVKG 325
           +   + YFG+G  SEGD HA  NFAA LE P+IF CRNNG+AISTP S+Q+R DG+  +G
Sbjct: 229 NRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARG 288

Query: 326 QAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPA 385
             YG+ SIRVDGND  AVYNA + AR  A+ E +P LIEA+TYR GHH+TSDDS+ YR  
Sbjct: 289 PGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSV 348

Query: 386 EEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLRKQLLEEIQLAERSEKPPVADA 445
           +E+ +W     P+SR R ++ S GWW  E E   R + R++++E  + AER  KP     
Sbjct: 349 DEVNYWDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERKPKPNPNLL 408

Query: 446 FTDVYDVPPSNLQEQEKWLRKTIKTHPQDYP 477
           F+DVY   P+ L++Q++ L + ++T+ + YP
Sbjct: 409 FSDVYQEMPAQLRKQQESLARHLQTYGEHYP 439

BLAST of CSPI06G26360 vs. ExPASy TrEMBL
Match: A0A0A0KF52 (E1_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483270 PE=4 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 4.4e-283
Identity = 479/481 (99.58%), Postives = 480/481 (99.79%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP
Sbjct: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA
Sbjct: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420
           ILIEALTYRAGHHTTSDDSTRYRPA+EMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAQEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420

Query: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFP 480
           SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPS FP
Sbjct: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of CSPI06G26360 vs. ExPASy TrEMBL
Match: A0A5A7SMQ8 (2-oxoisovalerate dehydrogenase subunit alpha 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1230G00070 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.1e-277
Identity = 470/481 (97.71%), Postives = 474/481 (98.54%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAFQVRKATTKIIPHFRNGM FFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THLFSRRFESIKVGKQL+CVNDDRD+QVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNG+P MYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA
Sbjct: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDKKDACAV YFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTP+SDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420
           ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAE ELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETELR 420

Query: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFP 480
           S LRKQLLEEIQLAER EKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPS FP
Sbjct: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of CSPI06G26360 vs. ExPASy TrEMBL
Match: A0A1S3B380 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103485287 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.1e-277
Identity = 470/481 (97.71%), Postives = 474/481 (98.54%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAFQVRKATTKIIPHFRNGM FFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THLFSRRFESIKVGKQL+CVNDDRD+QVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNG+P MYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA
Sbjct: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDKKDACAV YFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTP+SDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420
           ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAE ELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETELR 420

Query: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFP 480
           S LRKQLLEEIQLAER EKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPS FP
Sbjct: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of CSPI06G26360 vs. ExPASy TrEMBL
Match: A0A6J1BTD3 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111005384 PE=4 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 9.9e-243
Identity = 416/482 (86.31%), Postives = 449/482 (93.15%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNS-YSTHLRNTSFSAHKIGTRILGSENM 60
           MAFQ RKA TKIIPHF N M  FLG +EMGF +S Y+T L  T FS  KIG  ++G E+M
Sbjct: 1   MAFQARKA-TKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDM 60

Query: 61  PTHLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVL 120
           PTHL +RRF+SIKV K+L+ VNDDRD+QVLEFPGGKV+FT  +SFIPDR++ERIPCFRVL
Sbjct: 61  PTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVL 120

Query: 121 DDNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINV 180
           DDNG+  ++SNFTQVSKEVA+K+Y+DMVTLQTMDTI YEAQRQGRISFY+TS+GEEAIN+
Sbjct: 121 DDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINI 180

Query: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNY 240
           ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NY
Sbjct: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNY 240

Query: 241 FTVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 300
           FTVASTIASQIPHAVGAAYSMKM+KKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF
Sbjct: 241 FTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 300

Query: 301 ICRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360
           ICRNNGWAISTPISDQFRSDGV+VKG+AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR
Sbjct: 301 ICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360

Query: 361 PILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAEL 420
           PILIEALTYR GHHTTSDDSTRYRPAEE+EWWRVA DPVSRFRKWIESNGWWSNEAE+EL
Sbjct: 361 PILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESEL 420

Query: 421 RSRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKF 480
           RS LRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPS F
Sbjct: 421 RSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 480

Query: 481 PL 482
           PL
Sbjct: 481 PL 481

BLAST of CSPI06G26360 vs. ExPASy TrEMBL
Match: A0A6J1GE31 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111453112 PE=4 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.9e-242
Identity = 416/481 (86.49%), Postives = 441/481 (91.68%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAF VRKA TKIIPHFRNGM  FLG             LR T F+ HKIG R+LG ENM 
Sbjct: 1   MAFHVRKA-TKIIPHFRNGMR-FLG------------SLRETPFAVHKIGNRVLGFENML 60

Query: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
            HL + RF+S KV KQ++ VNDDRD+QVL FPGGKVKFT QLSF+P+REEER+PCFRVLD
Sbjct: 61  NHLSTHRFKSTKVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERVPCFRVLD 120

Query: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNG+P MYSNFTQVSKE+AMK+Y+DMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAIN+A
Sbjct: 121 DNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINIA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVL+WRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKM+K+DACAVTYFGDGGTSEGDFHAALNFAAVLE PVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTPISDQFRSDGVVVKG+AYGVRSIRVDGNDALAVYN VRAAR+MAIQE+RP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVRAARKMAIQERRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420
           ILIEALTYR GHHTTSDDSTRYRP EEMEWWRVA DPVSRFRKWIESNGWWS+EAEAELR
Sbjct: 361 ILIEALTYRVGHHTTSDDSTRYRPVEEMEWWRVAKDPVSRFRKWIESNGWWSDEAEAELR 420

Query: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFP 480
           S LRKQLLEEIQLAER+EKPPVADAFTDVYDVPPSNL+EQE+WLRKTIKTHPQDYPS F 
Sbjct: 421 SSLRKQLLEEIQLAERTEKPPVADAFTDVYDVPPSNLEEQERWLRKTIKTHPQDYPSDFQ 467

Query: 481 L 482
           L
Sbjct: 481 L 467

BLAST of CSPI06G26360 vs. NCBI nr
Match: XP_004148662.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial isoform X1 [Cucumis sativus] >KGN48345.1 hypothetical protein Csa_003053 [Cucumis sativus])

HSP 1 Score: 983.0 bits (2540), Expect = 9.1e-283
Identity = 479/481 (99.58%), Postives = 480/481 (99.79%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP
Sbjct: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA
Sbjct: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420
           ILIEALTYRAGHHTTSDDSTRYRPA+EMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAQEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420

Query: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFP 480
           SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPS FP
Sbjct: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of CSPI06G26360 vs. NCBI nr
Match: XP_008441060.1 (PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucumis melo] >KAA0025521.1 2-oxoisovalerate dehydrogenase subunit alpha 2 [Cucumis melo var. makuwa] >TYK25677.1 2-oxoisovalerate dehydrogenase subunit alpha 2 [Cucumis melo var. makuwa])

HSP 1 Score: 964.1 bits (2491), Expect = 4.4e-277
Identity = 470/481 (97.71%), Postives = 474/481 (98.54%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAFQVRKATTKIIPHFRNGM FFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THLFSRRFESIKVGKQL+CVNDDRD+QVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNG+P MYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA
Sbjct: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDKKDACAV YFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTP+SDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420
           ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAE ELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETELR 420

Query: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFP 480
           S LRKQLLEEIQLAER EKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPS FP
Sbjct: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of CSPI06G26360 vs. NCBI nr
Match: XP_038883947.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 905.6 bits (2339), Expect = 1.8e-259
Identity = 443/481 (92.10%), Postives = 459/481 (95.43%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           M F VRKATTKIIPHFRNGM  FLG LEMGFFNSYSTHLRNTSF+A+KIGT  L SE+MP
Sbjct: 1   MVFHVRKATTKIIPHFRNGMS-FLGSLEMGFFNSYSTHLRNTSFAAYKIGTGALDSEDMP 60

Query: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THL +RRFESIK GKQL+ VNDD D+QVLEFPGGKVKFTSQLSFIP REEERIPCFRVLD
Sbjct: 61  THLSARRFESIKAGKQLDSVNDDCDNQVLEFPGGKVKFTSQLSFIPYREEERIPCFRVLD 120

Query: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNG+P MYSNFTQVSKEVAMK+Y DMVTLQTMDTIFYEAQRQGRISFY+TSLGEEAIN+A
Sbjct: 121 DNGQPSMYSNFTQVSKEVAMKMYSDMVTLQTMDTIFYEAQRQGRISFYLTSLGEEAINIA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALS DDLIVPQYREAGVLIWRGFTLKEFASQCF NKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSFDDLIVPQYREAGVLIWRGFTLKEFASQCFSNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDK+DACAVTYFGDGGTSEGDFH ALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKRDACAVTYFGDGGTSEGDFHGALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTPISDQFRSDGVVVKG+AYGVRSIR+DGNDALAVYNAVRAAR+MAIQE +P
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRIDGNDALAVYNAVRAARKMAIQEHKP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420
           ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVA DPVSRFRKWIESNGWW+NEAEAELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAKDPVSRFRKWIESNGWWNNEAEAELR 420

Query: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFP 480
           S LRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPS FP
Sbjct: 421 SSLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 480

BLAST of CSPI06G26360 vs. NCBI nr
Match: XP_023543630.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 849.7 bits (2194), Expect = 1.2e-242
Identity = 417/481 (86.69%), Postives = 442/481 (91.89%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAF VRKA TKIIPHFRNGM  FLG             LR T F+ HKIG ++LG ENM 
Sbjct: 1   MAFHVRKA-TKIIPHFRNGMR-FLG------------SLRETPFAVHKIGNKVLGFENML 60

Query: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
            HL + RF+S KV KQ++ VNDDRD+QVL FPGGKVKFT QLSF+P+REEER+PCFRVLD
Sbjct: 61  NHLSAHRFKSTKVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERVPCFRVLD 120

Query: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNG+P M+SNFTQVSKE+AMK+Y+DMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAIN+A
Sbjct: 121 DNGQPSMFSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINIA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVL+WRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKM+K+DACAVTYFGDGGTSEGDFHAALNFAAVLE PVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFI 300

Query: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTPISDQFRSDGVVVKG+AYGVRSIRVDGNDALAVYN VRAAR+MAIQEQRP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVRAARKMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420
           ILIEALTYR GHHTTSDDSTRYRP EEMEWWRVA DPVSRFRKWIESNGWWS+EAEAELR
Sbjct: 361 ILIEALTYRVGHHTTSDDSTRYRPVEEMEWWRVAKDPVSRFRKWIESNGWWSDEAEAELR 420

Query: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFP 480
           S LRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNL+EQE+WLRKTIKTHPQDYPS FP
Sbjct: 421 SSLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLEEQERWLRKTIKTHPQDYPSDFP 467

Query: 481 L 482
           L
Sbjct: 481 L 467

BLAST of CSPI06G26360 vs. NCBI nr
Match: XP_022132554.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Momordica charantia])

HSP 1 Score: 849.0 bits (2192), Expect = 2.0e-242
Identity = 416/482 (86.31%), Postives = 449/482 (93.15%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNS-YSTHLRNTSFSAHKIGTRILGSENM 60
           MAFQ RKA TKIIPHF N M  FLG +EMGF +S Y+T L  T FS  KIG  ++G E+M
Sbjct: 1   MAFQARKA-TKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDM 60

Query: 61  PTHLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVL 120
           PTHL +RRF+SIKV K+L+ VNDDRD+QVLEFPGGKV+FT  +SFIPDR++ERIPCFRVL
Sbjct: 61  PTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVL 120

Query: 121 DDNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINV 180
           DDNG+  ++SNFTQVSKEVA+K+Y+DMVTLQTMDTI YEAQRQGRISFY+TS+GEEAIN+
Sbjct: 121 DDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINI 180

Query: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNY 240
           ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NY
Sbjct: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNY 240

Query: 241 FTVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 300
           FTVASTIASQIPHAVGAAYSMKM+KKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF
Sbjct: 241 FTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 300

Query: 301 ICRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360
           ICRNNGWAISTPISDQFRSDGV+VKG+AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR
Sbjct: 301 ICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360

Query: 361 PILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAEL 420
           PILIEALTYR GHHTTSDDSTRYRPAEE+EWWRVA DPVSRFRKWIESNGWWSNEAE+EL
Sbjct: 361 PILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESEL 420

Query: 421 RSRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKF 480
           RS LRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPS F
Sbjct: 421 RSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 480

Query: 481 PL 482
           PL
Sbjct: 481 PL 481

BLAST of CSPI06G26360 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 625.9 bits (1613), Expect = 2.6e-179
Identity = 292/395 (73.92%), Postives = 351/395 (88.86%), Query Frame = 0

Query: 87  QVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGRPCMYSNFTQVSKEVAMKIYHDM 146
           QV++FPGGKV FT ++ FI + ++ER+PC+RVLDDNG+    S F QVS+EVA+KIY DM
Sbjct: 78  QVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDM 137

Query: 147 VTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRGF 206
           VTLQ MD IFYEAQRQGR+SFY T++GEEAIN+ASAAAL+  D+I PQYRE GVL+WRGF
Sbjct: 138 VTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGF 197

Query: 207 TLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKD 266
           TL+EFA+QCFGNK DY KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KMDKKD
Sbjct: 198 TLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDKKD 257

Query: 267 ACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVVVKGQ 326
           ACAVTYFGDGGTSEGDFHAALN AAV+EAPV+FICRNNGWAISTP SDQFRSDGVVVKG+
Sbjct: 258 ACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVKGR 317

Query: 327 AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPAE 386
           AYG+RSIRVDGNDALA+Y+AV  AREMAI+EQRPILIEALTYR GHH+TSDDSTRYR A 
Sbjct: 318 AYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAG 377

Query: 387 EMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLRKQLLEEIQLAERSEKPPVADAF 446
           E+EWW  A +P+SRFR WIESNGWWS++ E++LRSR++K++LE +++AE++EKP + + F
Sbjct: 378 EIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQNMF 437

Query: 447 TDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFPL 482
           +DVYDVPPSNL+EQE  +R+TI +HPQDYPS  PL
Sbjct: 438 SDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

BLAST of CSPI06G26360 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 625.9 bits (1613), Expect = 2.6e-179
Identity = 292/395 (73.92%), Postives = 351/395 (88.86%), Query Frame = 0

Query: 87  QVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGRPCMYSNFTQVSKEVAMKIYHDM 146
           QV++FPGGKV FT ++ FI + ++ER+PC+RVLDDNG+    S F QVS+EVA+KIY DM
Sbjct: 7   QVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDM 66

Query: 147 VTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRGF 206
           VTLQ MD IFYEAQRQGR+SFY T++GEEAIN+ASAAAL+  D+I PQYRE GVL+WRGF
Sbjct: 67  VTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGF 126

Query: 207 TLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKD 266
           TL+EFA+QCFGNK DY KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KMDKKD
Sbjct: 127 TLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDKKD 186

Query: 267 ACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVVVKGQ 326
           ACAVTYFGDGGTSEGDFHAALN AAV+EAPV+FICRNNGWAISTP SDQFRSDGVVVKG+
Sbjct: 187 ACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVKGR 246

Query: 327 AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPAE 386
           AYG+RSIRVDGNDALA+Y+AV  AREMAI+EQRPILIEALTYR GHH+TSDDSTRYR A 
Sbjct: 247 AYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAG 306

Query: 387 EMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLRKQLLEEIQLAERSEKPPVADAF 446
           E+EWW  A +P+SRFR WIESNGWWS++ E++LRSR++K++LE +++AE++EKP + + F
Sbjct: 307 EIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQNMF 366

Query: 447 TDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKFPL 482
           +DVYDVPPSNL+EQE  +R+TI +HPQDYPS  PL
Sbjct: 367 SDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 401

BLAST of CSPI06G26360 vs. TAIR 10
Match: AT1G21400.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 589.3 bits (1518), Expect = 2.7e-168
Identity = 272/398 (68.34%), Postives = 337/398 (84.67%), Query Frame = 0

Query: 82  DDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGRPCMYSNFTQVSKEVAMK 141
           D+ D Q L+FPGGKV +TS++ FIP+    RIPC+RVLD++GR    S+F  VS+++A++
Sbjct: 73  DEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVR 132

Query: 142 IYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVL 201
           +Y  M TLQ MD IFYEAQRQGRISFY+TS+GEEAIN+ASAAALS DD+++PQYRE GVL
Sbjct: 133 MYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVL 192

Query: 202 IWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMK 261
           +WRGFTL+EFA+QCFGNK DY KGRQMP+HYGS R NYFT++S IA+Q+P A G  YS+K
Sbjct: 193 LWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTISSPIATQLPQAAGVGYSLK 252

Query: 262 MDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGV 321
           MDKK+AC VT+ GDGGTSEGDFHA LNFAAV+EAPV+FICRNNGWAIST IS+QFRSDG+
Sbjct: 253 MDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHISEQFRSDGI 312

Query: 322 VVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTR 381
           VVKGQAYG+RSIRVDGNDALAVY+AVR+AREMA+ EQRP+LIE +TYR GHH+TSDDST+
Sbjct: 313 VVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPVLIEMMTYRVGHHSTSDDSTK 372

Query: 382 YRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLRKQLLEEIQLAERSEKPP 441
           YR A+E+++W+++ +PV+RFRKW+E NGWWS E E++LRS  RKQLL+ IQ AE+ EK P
Sbjct: 373 YRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSNARKQLLQAIQAAEKWEKQP 432

Query: 442 VADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSKF 480
           + + F DVYDV P NL+EQE  L++ +K  PQDYP  F
Sbjct: 433 LTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYPPGF 470

BLAST of CSPI06G26360 vs. TAIR 10
Match: AT5G34780.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 313.5 bits (802), Expect = 2.9e-85
Identity = 146/222 (65.77%), Postives = 185/222 (83.33%), Query Frame = 0

Query: 254 VGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPIS 313
           +G + +    +K+ACAVT+ GDGGTSEGDFHA LNFAAV+EAPV+FICRNNGWAIST IS
Sbjct: 15  IGLSKAKDCWEKNACAVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHIS 74

Query: 314 DQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHH 373
           +QFRSDG+VVKGQAYG+RSIRVDGNDALAVY+AV +AREMA+ EQRP+LIE + YR GHH
Sbjct: 75  EQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVCSAREMAVTEQRPVLIEMMIYRVGHH 134

Query: 374 TTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLRKQLLEEIQL 433
           +TSDDST+YR A+E+++W+++ + V+RFRK +E NGWWS E E++LRS  RKQLL+ IQ 
Sbjct: 135 STSDDSTKYRAADEIQYWKMSRNSVNRFRKSVEDNGWWSEEDESKLRSNARKQLLQAIQA 194

Query: 434 AERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDY 476
           AE+ EK P+ + F DVYDV P NL+E+E  L++ I+  PQDY
Sbjct: 195 AEKWEKQPLTELFNDVYDVKPKNLEEEELGLKELIEKQPQDY 236

BLAST of CSPI06G26360 vs. TAIR 10
Match: AT1G24180.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 147.5 bits (371), Expect = 2.7e-35
Identity = 92/326 (28.22%), Postives = 162/326 (49.69%), Query Frame = 0

Query: 129 SNFTQVSKEVAMKIYHDMVTLQTM----DTIFYEAQRQGRISFYVTSLGEEAINVASAAA 188
           S   + S E  +  + DM  ++ M    D+++     +G    Y    G+EA+ V   AA
Sbjct: 53  SRSVETSSEEILAFFRDMARMRRMEIAADSLYKAKLIRGFCHLYD---GQEALAVGMEAA 112

Query: 189 LSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVAS 248
           ++  D I+  YR+    I RG  L +  S+  G K     G+   +H+  K  +++    
Sbjct: 113 ITKKDAIITSYRDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDASFYGGHG 172

Query: 249 TIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNN 308
            + +QIP   G A++ K +K +A     +GDG  ++G    ALN +A+ + P I +C NN
Sbjct: 173 IVGAQIPLGCGLAFAQKYNKDEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENN 232

Query: 309 GWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIE 368
            + + T      +S     +G    V  ++VDG DALAV  A + A+E A+ +  PI++E
Sbjct: 233 HYGMGTATWRSAKSPAYFKRGDY--VPGLKVDGMDALAVKQACKFAKEHAL-KNGPIILE 292

Query: 369 ALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELRSRLR 428
             TYR   H+ SD  + YR  +E+   R   DP+ R RK + ++   + +   ++   +R
Sbjct: 293 MDTYRYHGHSMSDPGSTYRTRDEISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIR 352

Query: 429 KQLLEEIQLAERSEKPPVADAFTDVY 451
           K++ + +  A+ S  P  ++ FT++Y
Sbjct: 353 KEVDDAVAQAKESPIPDASELFTNMY 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84JL23.7e-17873.922-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis tha... [more]
Q9LPL53.8e-16768.342-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis tha... [more]
A5A6H97.3e-11049.362-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes O... [more]
P501361.6e-10948.592-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus OX=1... [more]
P126942.1e-10948.852-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Homo sapiens OX=9... [more]
Match NameE-valueIdentityDescription
A0A0A0KF524.4e-28399.58E1_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483270 PE=4 ... [more]
A0A5A7SMQ82.1e-27797.712-oxoisovalerate dehydrogenase subunit alpha 2 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3B3802.1e-27797.712-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucumis melo OX... [more]
A0A6J1BTD39.9e-24386.312-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Momordica chara... [more]
A0A6J1GE314.9e-24286.492-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucurbita mosch... [more]
Match NameE-valueIdentityDescription
XP_004148662.19.1e-28399.582-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial isoform X1 [Cucumi... [more]
XP_008441060.14.4e-27797.71PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucumi... [more]
XP_038883947.11.8e-25992.102-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Benincasa hispida... [more]
XP_023543630.11.2e-24286.692-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita pepo su... [more]
XP_022132554.12.0e-24286.312-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Momordica charant... [more]
Match NameE-valueIdentityDescription
AT5G09300.12.6e-17973.92Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G09300.22.6e-17973.92Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT1G21400.12.7e-16868.34Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G34780.12.9e-8565.77Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT1G24180.12.7e-3528.22Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.970coord: 52..447
e-value: 1.3E-145
score: 487.3
NoneNo IPR availablePANTHERPTHR433802-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIALcoord: 43..449
NoneNo IPR availablePANTHERPTHR43380:SF112-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIALcoord: 43..449
NoneNo IPR availableCDDcd02000TPP_E1_PDC_ADC_BCADCcoord: 110..402
e-value: 3.78623E-148
score: 421.519
IPR001017Dehydrogenase, E1 componentPFAMPF00676E1_dhcoord: 114..409
e-value: 4.9E-94
score: 314.6
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 81..444

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G26360.1CSPI06G26360.1mRNA
CSPI06G26360.2CSPI06G26360.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009083 branched-chain amino acid catabolic process
cellular_component GO:0005947 mitochondrial alpha-ketoglutarate dehydrogenase complex
molecular_function GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor