CSPI06G25200 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G25200
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Splicing factor U2af small subunit B-like
Location	Chr6: 22458589 .. 22461775 (+)
RNA-Seq Expression	CSPI06G25200
Synteny	CSPI06G25200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGAGGAGAAGGAAAGCAAGACTCTGCCGGTCTCGAGGAAAAGAAAATGGCGGAACACTTGGCTTCAATTTTCGGAACGGAGAAGGATAGGGTTAACTGCCCTTTCTACTTCAAGATTGGAGCCTGTAGACATGGTGATCGGTGCTCTAGGCTTCACACTAAGCCCAGCATCAGCCCTACACTCCTCCTCTCTAATATGTATCAGAGACCCGATATGATCACCCCCGGCGTCGACCCTCAAGGCCAAGCTCTTGACCCTCGCAAGGTTCAGGATCACTTTGAGGTATTAAATCTCTGCCGTTTCTATCTTTTTTCGTTGATTGGCATTTGTTTCTTGGACTTTTTAACCTTCAATCTTTTCAACTGAAATGGTTTTCTTCCTTTCCTTCTTTTTTGCAATCTGGAAATGTAGGACTTTTATGAAGATCTATTTGAGGAGTTGAGCAAATATGGGGATCTTGAAAGTTTAAATATTTGTGATAATCTGGCCGACCATATGGTATGTTATCTCTCGCGTTTTAAATTTAAGGGTGTATTTGGATCTGTGTTACTCTGACTAGTTTGGTTTTAAATCCATGGACAGGTGGGCAATGTCTATGTTCAGTTTAGAGAAGAAGAACAAGCTGCAAATGCTCTTCACAATCTTAATGGACGCTTTTATGCAGGTTTATTGTCCTATACTTTTTTTGTTTATTTTCTTTTTCCTTTAGGGTTTTCTTTTTACAATGAAATGAATCTTGTGGGTGCTATAGGTGTACAATAAGATGAAGCTTCGTGTACATCAGTGATTGGTTTATTCAATGCTGATTATGTAGTTATATTATTACGAGTTTGATGTGAAATTTCTAAATCAATAATGACGTTGCTAGTGTTTGGTTTGGAGGTTTAATGGCGCTTTCCCAAAGGGAGTTAACTGTAAACTTGGATTAACTGTATTTTTCAAAATTTATATGTGCTATTCAATTATCAATTATCATATCAACGGATATACTCTTGATCAATGGTTGCTAATAGAAAAAATATGGTTGCTTTACACTCTTTGTTATTTGTTTGTGTGTAAGATGGCCCAGGTATGTAGTTACTAGGTCTTATTTTTCAAATTTTTGATAGGTCGACCCATCATTGTTGACTTCTCTCCTGTGACGGATTTTCGAGAAGCTACTTGCAGGCAGTATGAGGAAAATGTATGTAACCGTGGTGGCTATTGTAACTTTATGCATCTGAAGAAGATAAGCAGGTACATCATCACCACAATTCCTTTGTTTTTCCTGAAAATGTATTGGTTAGCTAATGATCCACATGCTTATTGTTGTAATTTTTCATGTGGTTTTAGAGAGTTGAGAAGGCGCTTATTTGGGAGGAGCAGGCGCAGGCGTAGCCGAAGCCGAAGCCGAAGTCAAAGCCCCCACAAACACCATGGATATGAAGAACGTTCCCATGGTGGTGGTGGTGGTCGTGGACGTGGGCCTAGTAGAAGAGATGGAGAAAAGGACCCTCGCTATCATGATAGAAGCAGGAGGCCTAGAAGCCGTAGCCCTAGGCATAGAGGGGGACGGAGTAGAAGCCCTGGAGGGAGGAGAAACAGAAGTCCAGTTAGGGAAAGTAGTGCTGAAAGAAGGGCTAAGATTGAACAGTGGAACAGGGACAGGGAAAAGGATGATAATGGTAGTAATCATCAGGATGTCAAGGATGTTGGTGGAAGCAATGACCTAGTACAAAATGAAGATGAATTTGATCCTTCTAAGCAGTTCTAGAGAAAATGGTGGATGCGGTGCTTGCCTGAGAAAAATTTTCTGGTTTGCTTCTGTCCTTTTATTGGTTCAATGTAGGGGCCATAGTAGAATGCTTTCCCCTTTATTAATAAAACAGCCCATTCTCTAAAAGAAGTAGCTCACTGCCCATTTCCACTGTGCAGGTGGAGGACAGGTTCCCTGTCACGTGTTATTGAATGTGAATTCCAGTTTTGTTAACCTCATCCCTTTCACCCTAAAAATGAAAATAAATAGGATAGAAATAATCTTTAGTGAGTAACCCTCTTGGAAAACTCTTCAATATTCCATACCATTTTGAATTGGTCACAGCCATCCTCATTCATTGTTCTTTGGTCGTTCACCTTAGTCGGAATGTGTTTCCCCATGTTTATGGGAATTACAGGATGAAATAAAACCTTAGATCACCTAAAAGTAGGATTATTGTATGTAGTGCTTAACCTGCAAAAGTCTAGAGGAAAGGTTTTTTACAAACCATTTTTTGTTTTCTTTACTGTTTTTGTACTTTTCTTAAACTTTCTTTTCATCACTTTCACATGAAGAGCAAGACCAAGATTTTAGGACCGGCCTTCATGTTTGCTGATGGTTACCACTTTCTTAATTATACAGGTTAATCTATGGAAAAAATTGTGCGTTTATCGTATTATTTTCTACGCCTCAAAATTTACTCAACATTTTCTTCAATTGGGTTTCTTGAGTCAACATTTTTAAACTAGTATTTTTCTAAAAACTTCTTGTAGACATCCTTACCTAAAGTAGATGTAGAGCTTGTTGTCAGCCTAATTGAATGTTATTCCCTTTCACTCTCATCTTTACAGGAAGAAATAGAATTTTAGAAGCGGTTGTGGTCATGGCTTTTTATTATTTGTATGCAGATTATGGATATTGAAATTTCCTTTTATGTTTCTTACATATTCTGCGAGTTTTGCAGGTTCAGGTTTTCCTGCTTGCCCGAATACTTCTTAGGCAGCTTACCATCGTATTCATAATACAGGTTTGGCTGTTATCTGGAGGATTTGCAGGATTTGATGTGATGTTTCGTTGGAAACATGAAGGAAGCTTCTCTCCACAACTTCTGCAATGCTGAAAAATGTATGTCCTCGTTCGGTTGATTTTTCCCCATCTCATAATGAAGTAAGTTTGAGGATTTCTGAGACACAAGTAGATTTAATGTTGTTTTCTTGTTTTAAATCGTGTTGTATTTCCAAGATTTTATTGACGTTTTCAGAGAGAGATACTATTGAGAGCTGCCTACGATAATAGTTAAAGTGTCCTTTGGTGACCTTGATATCTTGGACTTCTCATCTTCCTTGTATTGCCTTGTGTGCTAAGGTTTTCTCTTCTTCTTTATTTTGAATTGGACCATCTTAAACTTATACCTCTTTTGAACTGTAAAAGTATCTGTGTCATTTTATAG

mRNA sequence

CAGGAGGAGAAGGAAAGCAAGACTCTGCCGGTCTCGAGGAAAAGAAAATGGCGGAACACTTGGCTTCAATTTTCGGAACGGAGAAGGATAGGGTTAACTGCCCTTTCTACTTCAAGATTGGAGCCTGTAGACATGGTGATCGGTGCTCTAGGCTTCACACTAAGCCCAGCATCAGCCCTACACTCCTCCTCTCTAATATGTATCAGAGACCCGATATGATCACCCCCGGCGTCGACCCTCAAGGCCAAGCTCTTGACCCTCGCAAGGTTCAGGATCACTTTGAGGACTTTTATGAAGATCTATTTGAGGAGTTGAGCAAATATGGGGATCTTGAAAGTTTAAATATTTGTGATAATCTGGCCGACCATATGGTGGGCAATGTCTATGTTCAGTTTAGAGAAGAAGAACAAGCTGCAAATGCTCTTCACAATCTTAATGGACGCTTTTATGCAGGTCGACCCATCATTGTTGACTTCTCTCCTGTGACGGATTTTCGAGAAGCTACTTGCAGGCAGTATGAGGAAAATGTATGTAACCGTGGTGGCTATTGTAACTTTATGCATCTGAAGAAGATAAGCAGAGAGTTGAGAAGGCGCTTATTTGGGAGGAGCAGGCGCAGGCGTAGCCGAAGCCGAAGCCGAAGTCAAAGCCCCCACAAACACCATGGATATGAAGAACGTTCCCATGGTGGTGGTGGTGGTCGTGGACGTGGGCCTAGTAGAAGAGATGGAGAAAAGGACCCTCGCTATCATGATAGAAGCAGGAGGCCTAGAAGCCGTAGCCCTAGGCATAGAGGGGGACGGAGTAGAAGCCCTGGAGGGAGGAGAAACAGAAGTCCAGTTAGGGAAAGTAGTGCTGAAAGAAGGGCTAAGATTGAACAGTGGAACAGGGACAGGGAAAAGGATGATAATGGTAGTAATCATCAGGATGTCAAGGATGTTGGTGGAAGCAATGACCTAGTACAAAATGAAGATGAATTTGATCCTTCTAAGCAGTTCTAGAGAAAATGGTGGATGCGGTGCTTGCCTGAGAAAAATTTTCTGGTTTGGCTGTTATCTGGAGGATTTGCAGGATTTGATGTGATGTTTCGTTGGAAACATGAAGGAAGCTTCTCTCCACAACTTCTGCAATGCTGAAAAATGTATGTCCTCGTTCGGTTGATTTTTCCCCATCTCATAATGAAGTAAGTTTGAGGATTTCTGAGACACAAGTAGATTTAATGTTGTTTTCTTGTTTTAAATCGTGTTGTATTTCCAAGATTTTATTGACGTTTTCAGAGAGAGATACTATTGAGAGCTGCCTACGATAATAGTTAAAGTGTCCTTTGGTGACCTTGATATCTTGGACTTCTCATCTTCCTTGTATTGCCTTGTGTGCTAAGGTTTTCTCTTCTTCTTTATTTTGAATTGGACCATCTTAAACTTATACCTCTTTTGAACTGTAAAAGTATCTGTGTCATTTTATAG

Coding sequence (CDS)

ATGGCGGAACACTTGGCTTCAATTTTCGGAACGGAGAAGGATAGGGTTAACTGCCCTTTCTACTTCAAGATTGGAGCCTGTAGACATGGTGATCGGTGCTCTAGGCTTCACACTAAGCCCAGCATCAGCCCTACACTCCTCCTCTCTAATATGTATCAGAGACCCGATATGATCACCCCCGGCGTCGACCCTCAAGGCCAAGCTCTTGACCCTCGCAAGGTTCAGGATCACTTTGAGGACTTTTATGAAGATCTATTTGAGGAGTTGAGCAAATATGGGGATCTTGAAAGTTTAAATATTTGTGATAATCTGGCCGACCATATGGTGGGCAATGTCTATGTTCAGTTTAGAGAAGAAGAACAAGCTGCAAATGCTCTTCACAATCTTAATGGACGCTTTTATGCAGGTCGACCCATCATTGTTGACTTCTCTCCTGTGACGGATTTTCGAGAAGCTACTTGCAGGCAGTATGAGGAAAATGTATGTAACCGTGGTGGCTATTGTAACTTTATGCATCTGAAGAAGATAAGCAGAGAGTTGAGAAGGCGCTTATTTGGGAGGAGCAGGCGCAGGCGTAGCCGAAGCCGAAGCCGAAGTCAAAGCCCCCACAAACACCATGGATATGAAGAACGTTCCCATGGTGGTGGTGGTGGTCGTGGACGTGGGCCTAGTAGAAGAGATGGAGAAAAGGACCCTCGCTATCATGATAGAAGCAGGAGGCCTAGAAGCCGTAGCCCTAGGCATAGAGGGGGACGGAGTAGAAGCCCTGGAGGGAGGAGAAACAGAAGTCCAGTTAGGGAAAGTAGTGCTGAAAGAAGGGCTAAGATTGAACAGTGGAACAGGGACAGGGAAAAGGATGATAATGGTAGTAATCATCAGGATGTCAAGGATGTTGGTGGAAGCAATGACCTAGTACAAAATGAAGATGAATTTGATCCTTCTAAGCAGTTCTAG

Protein sequence

MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEEQAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISRELRRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRRPRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGGSNDLVQNEDEFDPSKQF*

Homology

[Close]

BLAST of CSPI06G25200 vs. ExPASy Swiss-Prot
Match: Q9FMY5 (Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B PE=1 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 9.4e-106
Identity = 206/293 (70.31%), Postives = 232/293 (79.18%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQ LDP K+QDHFEDFYED+FEEL+K+G++ESLN+CDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
            AA AL  L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNFMH+K+ISREL
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RRRLFGRSR---RRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDR 240
           RR+LFGR R   RR SRSRSRS SP +     +R H     RG         +D   H  
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRR-----KREHSRERERG-------DVRDRDRHGN 240

Query: 241 SRRPRSRSPRH---RGGRSRSPGGRRNRSP--VRESSAERRAKIEQWNRDREK 286
            +R   RS RH    GGR R    +R+RSP  VRE S ERRA+IEQWNR+R++
Sbjct: 241 GKRSSDRSERHDRDGGGRRRHGSPKRSRSPRNVREGSEERRARIEQWNRERDE 281

[Close]

BLAST of CSPI06G25200 vs. ExPASy Swiss-Prot
Match: Q9S709 (Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A PE=1 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 3.0e-104
Identity = 211/304 (69.41%), Postives = 234/304 (76.97%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVD QGQ LDPRK+Q+HFEDF+EDLFEEL K+G++ESLNICDNLADHM+GNVYVQF+EE+
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAA AL  L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNFMH+K +SREL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSREL 180

Query: 181 RRRLFGRSRR-----RRSRSRSRSQSPHKHHGYEER--SHGGGGGRGR-------GPSRR 240
           RR+LFGR RR      RSRSRSRS SP      + R  SH     R R       G  +R
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGKR 240

Query: 241 DGEKDPRY-HDRSRRPRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK 290
             E+  R   D SR  R  SP+ RGG   SPGG       RE S ERRA+IEQWNR+RE+
Sbjct: 241 SSERSERQERDGSRGRRQASPK-RGG---SPGGG------REGSEERRARIEQWNREREE 294

[Close]

BLAST of CSPI06G25200 vs. ExPASy Swiss-Prot
Match: Q6AUG0 (Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35B PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.2e-102
Identity = 200/303 (66.01%), Postives = 232/303 (76.57%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P++SPT++L+NMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTVSPTIVLANMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVD QGQ +DP K+Q+HFEDFYED++EELSK+G++E+LN+CDNLADHM+GNVYVQFREEE
Sbjct: 61  GVDAQGQPIDPEKMQEHFEDFYEDIYEELSKFGEVETLNVCDNLADHMIGNVYVQFREEE 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QA  A + L GRFY+GRPIIV++SPVTDFREATCRQ+EEN CNRGGYCNFMH+K+I REL
Sbjct: 121 QAVAAHNALQGRFYSGRPIIVEYSPVTDFREATCRQFEENSCNRGGYCNFMHVKQIGREL 180

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEER---------------SHGGGGGRGRGPSR 240
           RR+L+G  R RRS  RSRS SP    G  +R                + GGGG G G   
Sbjct: 181 RRKLYG-GRSRRSHGRSRSPSPRHRRGNRDRDDFRRERDGYRGGGDGYRGGGGGGGGDGY 240

Query: 241 RDGEKDPRYHDRSRR-PRSRSPRHRGGRSRSPGG--RRNRSPVRESSAERRAKIEQWNRD 286
           R G+         RR   SR  R+  G  R  G   RR RSPVRESS ERRAKIEQWNR+
Sbjct: 241 RGGDSYRGGGGGGRRGGGSRYDRYDDGGRRRHGSPPRRARSPVRESSEERRAKIEQWNRE 300

[Close]

BLAST of CSPI06G25200 vs. ExPASy Swiss-Prot
Match: Q9ZQW8 (Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35A PE=2 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.4e-101
Identity = 199/299 (66.56%), Postives = 224/299 (74.92%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH KPS+SPTLLLSNMY RPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNKPSVSPTLLLSNMYLRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           G+D QG  +DP K+Q  FEDFYED+FEELSKYG++ESL++CDN ADHM+GNVYVQFREE+
Sbjct: 61  GIDAQGNPIDPEKIQADFEDFYEDIFEELSKYGEIESLHVCDNFADHMIGNVYVQFREED 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAA AL  L GR+Y+GRPIIV+FSPV+DFREATCRQYEEN CNRGGYCNFMH+K+I R+L
Sbjct: 121 QAARALQALTGRYYSGRPIIVEFSPVSDFREATCRQYEENSCNRGGYCNFMHVKEIGRDL 180

Query: 181 RRRLFG---RSRRRRSRSRSRSQSP-HKHHGYEERS-----------HGGGGGRGRGPSR 240
           R+RLFG   RSRR  S  RSRS SP H    Y+ RS            GG     RG SR
Sbjct: 181 RKRLFGHLHRSRRSHSHGRSRSPSPYHYRRDYDRRSSSRSRDHDDYYRGGSHDYYRGGSR 240

Query: 241 RDGEKDPRYHDRSRRPRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDRE 285
           R  E+    +D     R    RHR          R RSPVR+ S ERRA+IEQWNR+RE
Sbjct: 241 RSSERHRSSYDSDGSRR----RHRS---------RTRSPVRDGSEERRAQIEQWNRERE 286

[Close]

BLAST of CSPI06G25200 vs. ExPASy Swiss-Prot
Match: Q9D883 (Splicing factor U2AF 35 kDa subunit OS=Mus musculus OX=10090 GN=U2af1 PE=1 SV=4)

HSP 1 Score: 265.0 bits (676), Expect = 1.1e-69
Identity = 134/241 (55.60%), Postives = 172/241 (71.37%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAE+LASIFGTEKD+VNC FYFKIGACRHGDRCSRLH KP+ S T+ L N+Y+ P   + 
Sbjct: 1   MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQ 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEEL-SKYGDLESLNICDNLADHMVGNVYVQFREE 120
             D    A+   ++Q+H+++F+E++F E+  KYG++E +N+CDNL DH+VGNVYV+FR E
Sbjct: 61  SADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRRE 120

Query: 121 EQAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISRE 180
           E A  A+ +LN R++ G+PI  + SPVTDFREA CRQYE   C RGG+CNFMHLK ISRE
Sbjct: 121 EDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRE 180

Query: 181 LRRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSR 240
           LRR L+G   RRR + RSRS+S  +     +R  GGGGG G G  R    +  R  +RS 
Sbjct: 181 LRRELYG---RRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSG 238

[Close]

BLAST of CSPI06G25200 vs. ExPASy TrEMBL
Match: A0A0A0KF96 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G450380 PE=4 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 5.6e-178
Identity = 316/317 (99.68%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL
Sbjct: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240
           RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR
Sbjct: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240

Query: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGG 300
           PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK+DNGSNHQDVKDVGG
Sbjct: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKEDNGSNHQDVKDVGG 300

Query: 301 SNDLVQNEDEFDPSKQF 318
           SNDLVQNEDEFDPSKQF
Sbjct: 301 SNDLVQNEDEFDPSKQF 317

[Close]

BLAST of CSPI06G25200 vs. ExPASy TrEMBL
Match: A0A1S3CH52 (splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC103500870 PE=4 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 9.3e-173
Identity = 312/317 (98.42%), Postives = 312/317 (98.42%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL
Sbjct: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240
           RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH  GGGRGRG SRRDGEKDPRY DRSRR
Sbjct: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH--GGGRGRGSSRRDGEKDPRYLDRSRR 240

Query: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGG 300
           PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK DNGSNHQDVKDVGG
Sbjct: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKADNGSNHQDVKDVGG 300

Query: 301 SNDLVQNEDEFDPSKQF 318
           SNDLVQNEDEFDPSKQF
Sbjct: 301 SNDLVQNEDEFDPSKQF 315

[Close]

BLAST of CSPI06G25200 vs. ExPASy TrEMBL
Match: A0A5D3C894 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00980 PE=3 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 9.3e-173
Identity = 312/317 (98.42%), Postives = 312/317 (98.42%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 460 MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 519

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE
Sbjct: 520 GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 579

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL
Sbjct: 580 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 639

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240
           RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH  GGGRGRG SRRDGEKDPRY DRSRR
Sbjct: 640 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH--GGGRGRGSSRRDGEKDPRYLDRSRR 699

Query: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGG 300
           PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK DNGSNHQDVKDVGG
Sbjct: 700 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKADNGSNHQDVKDVGG 759

Query: 301 SNDLVQNEDEFDPSKQF 318
           SNDLVQNEDEFDPSKQF
Sbjct: 760 SNDLVQNEDEFDPSKQF 774

[Close]

BLAST of CSPI06G25200 vs. ExPASy TrEMBL
Match: A0A5A7SM18 (Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001450 PE=4 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 9.3e-173
Identity = 312/317 (98.42%), Postives = 312/317 (98.42%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 26  MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 85

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE
Sbjct: 86  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 145

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL
Sbjct: 146 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 205

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240
           RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH  GGGRGRG SRRDGEKDPRY DRSRR
Sbjct: 206 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH--GGGRGRGSSRRDGEKDPRYLDRSRR 265

Query: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGG 300
           PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK DNGSNHQDVKDVGG
Sbjct: 266 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKADNGSNHQDVKDVGG 325

Query: 301 SNDLVQNEDEFDPSKQF 318
           SNDLVQNEDEFDPSKQF
Sbjct: 326 SNDLVQNEDEFDPSKQF 340

[Close]

BLAST of CSPI06G25200 vs. ExPASy TrEMBL
Match: A0A6J1EGF6 (splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111433144 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 4.9e-166
Identity = 298/317 (94.01%), Postives = 307/317 (96.85%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAANAL NLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL
Sbjct: 121 QAANALQNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240
           RRRLFGRSRRRRSRS+S+S+SPH+HHG+++R H   GGRGRGPSRRDG+KDPRYHDRSRR
Sbjct: 181 RRRLFGRSRRRRSRSQSQSRSPHRHHGHDDRPH---GGRGRGPSRRDGDKDPRYHDRSRR 240

Query: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGG 300
           PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDRE  DNGS HQDVKD GG
Sbjct: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDRETADNGSGHQDVKDDGG 300

Query: 301 SNDLVQNEDEFDPSKQF 318
           SN LVQNEDEFDPSKQ+
Sbjct: 301 SNGLVQNEDEFDPSKQY 314

[Close]

BLAST of CSPI06G25200 vs. NCBI nr
Match: XP_004143302.1 (splicing factor U2af small subunit B [Cucumis sativus] >XP_011657685.1 splicing factor U2af small subunit B [Cucumis sativus] >KGN48233.1 hypothetical protein Csa_003140 [Cucumis sativus])

HSP 1 Score: 633.3 bits (1632), Expect = 1.2e-177
Identity = 316/317 (99.68%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL
Sbjct: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240
           RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR
Sbjct: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240

Query: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGG 300
           PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK+DNGSNHQDVKDVGG
Sbjct: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKEDNGSNHQDVKDVGG 300

Query: 301 SNDLVQNEDEFDPSKQF 318
           SNDLVQNEDEFDPSKQF
Sbjct: 301 SNDLVQNEDEFDPSKQF 317

[Close]

BLAST of CSPI06G25200 vs. NCBI nr
Match: TYK07378.1 (enolase [Cucumis melo var. makuwa])

HSP 1 Score: 615.9 bits (1587), Expect = 1.9e-172
Identity = 312/317 (98.42%), Postives = 312/317 (98.42%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 460 MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 519

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE
Sbjct: 520 GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 579

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL
Sbjct: 580 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 639

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240
           RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH  GGGRGRG SRRDGEKDPRY DRSRR
Sbjct: 640 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH--GGGRGRGSSRRDGEKDPRYLDRSRR 699

Query: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGG 300
           PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK DNGSNHQDVKDVGG
Sbjct: 700 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKADNGSNHQDVKDVGG 759

Query: 301 SNDLVQNEDEFDPSKQF 318
           SNDLVQNEDEFDPSKQF
Sbjct: 760 SNDLVQNEDEFDPSKQF 774

[Close]

BLAST of CSPI06G25200 vs. NCBI nr
Match: XP_008462532.1 (PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >XP_008462533.1 PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo])

HSP 1 Score: 615.9 bits (1587), Expect = 1.9e-172
Identity = 312/317 (98.42%), Postives = 312/317 (98.42%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL
Sbjct: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240
           RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH  GGGRGRG SRRDGEKDPRY DRSRR
Sbjct: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH--GGGRGRGSSRRDGEKDPRYLDRSRR 240

Query: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGG 300
           PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK DNGSNHQDVKDVGG
Sbjct: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKADNGSNHQDVKDVGG 300

Query: 301 SNDLVQNEDEFDPSKQF 318
           SNDLVQNEDEFDPSKQF
Sbjct: 301 SNDLVQNEDEFDPSKQF 315

[Close]

BLAST of CSPI06G25200 vs. NCBI nr
Match: KAA0025291.1 (splicing factor U2af small subunit B-like [Cucumis melo var. makuwa])

HSP 1 Score: 615.9 bits (1587), Expect = 1.9e-172
Identity = 312/317 (98.42%), Postives = 312/317 (98.42%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 26  MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 85

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE
Sbjct: 86  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 145

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL
Sbjct: 146 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 205

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240
           RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH  GGGRGRG SRRDGEKDPRY DRSRR
Sbjct: 206 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSH--GGGRGRGSSRRDGEKDPRYLDRSRR 265

Query: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGG 300
           PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK DNGSNHQDVKDVGG
Sbjct: 266 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKADNGSNHQDVKDVGG 325

Query: 301 SNDLVQNEDEFDPSKQF 318
           SNDLVQNEDEFDPSKQF
Sbjct: 326 SNDLVQNEDEFDPSKQF 340

[Close]

BLAST of CSPI06G25200 vs. NCBI nr
Match: XP_038882602.1 (splicing factor U2af small subunit B-like [Benincasa hispida] >XP_038882603.1 splicing factor U2af small subunit B-like [Benincasa hispida])

HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 305/317 (96.21%), Postives = 312/317 (98.42%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL
Sbjct: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180

Query: 181 RRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDRSRR 240
           RRRLFGRSRRRRSRSRS+S+SPHKHHGYE+R H  GGGRGRGPSRR+G+KDPRYH+RSRR
Sbjct: 181 RRRLFGRSRRRRSRSRSQSRSPHKHHGYEDRPH--GGGRGRGPSRREGDKDPRYHERSRR 240

Query: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKDDNGSNHQDVKDVGG 300
           PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK DNGSNHQDVKDVGG
Sbjct: 241 PRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREKADNGSNHQDVKDVGG 300

Query: 301 SNDLVQNEDEFDPSKQF 318
           SNDL QNEDEFDPSKQ+
Sbjct: 301 SNDLAQNEDEFDPSKQY 315

[Close]

BLAST of CSPI06G25200 vs. TAIR 10
Match: AT5G42820.2 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 384.8 bits (987), Expect = 6.7e-107
Identity = 206/293 (70.31%), Postives = 232/293 (79.18%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQ LDP K+QDHFEDFYED+FEEL+K+G++ESLN+CDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
            AA AL  L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNFMH+K+ISREL
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RRRLFGRSR---RRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDR 240
           RR+LFGR R   RR SRSRSRS SP +     +R H     RG         +D   H  
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRR-----KREHSRERERG-------DVRDRDRHGN 240

Query: 241 SRRPRSRSPRH---RGGRSRSPGGRRNRSP--VRESSAERRAKIEQWNRDREK 286
            +R   RS RH    GGR R    +R+RSP  VRE S ERRA+IEQWNR+R++
Sbjct: 241 GKRSSDRSERHDRDGGGRRRHGSPKRSRSPRNVREGSEERRARIEQWNRERDE 281

[Close]

BLAST of CSPI06G25200 vs. TAIR 10
Match: AT5G42820.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 384.8 bits (987), Expect = 6.7e-107
Identity = 206/293 (70.31%), Postives = 232/293 (79.18%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVDPQGQ LDP K+QDHFEDFYED+FEEL+K+G++ESLN+CDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
            AA AL  L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNFMH+K+ISREL
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RRRLFGRSR---RRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDGEKDPRYHDR 240
           RR+LFGR R   RR SRSRSRS SP +     +R H     RG         +D   H  
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRR-----KREHSRERERG-------DVRDRDRHGN 240

Query: 241 SRRPRSRSPRH---RGGRSRSPGGRRNRSP--VRESSAERRAKIEQWNRDREK 286
            +R   RS RH    GGR R    +R+RSP  VRE S ERRA+IEQWNR+R++
Sbjct: 241 GKRSSDRSERHDRDGGGRRRHGSPKRSRSPRNVREGSEERRARIEQWNRERDE 281

[Close]

BLAST of CSPI06G25200 vs. TAIR 10
Match: AT1G27650.1 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 379.8 bits (974), Expect = 2.2e-105
Identity = 211/304 (69.41%), Postives = 234/304 (76.97%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEE 120
           GVD QGQ LDPRK+Q+HFEDF+EDLFEEL K+G++ESLNICDNLADHM+GNVYVQF+EE+
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISREL 180
           QAA AL  L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNFMH+K +SREL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSREL 180

Query: 181 RRRLFGRSRR-----RRSRSRSRSQSPHKHHGYEER--SHGGGGGRGR-------GPSRR 240
           RR+LFGR RR      RSRSRSRS SP      + R  SH     R R       G  +R
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGKR 240

Query: 241 DGEKDPRY-HDRSRRPRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAKIEQWNRDREK 290
             E+  R   D SR  R  SP+ RGG   SPGG       RE S ERRA+IEQWNR+RE+
Sbjct: 241 SSERSERQERDGSRGRRQASPK-RGG---SPGGG------REGSEERRARIEQWNREREE 294

[Close]

BLAST of CSPI06G25200 vs. TAIR 10
Match: AT1G27650.2 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 276.2 bits (705), Expect = 3.4e-74
Identity = 164/254 (64.57%), Postives = 185/254 (72.83%), Query Frame = 0


Query: 51  MYQRPDMITPGVDPQGQALDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVG 110
           MYQRPDMITPGVD QGQ LDPRK+Q+HFEDF+EDLFEEL K+G++ESLNICDNLADHM+G
Sbjct: 1   MYQRPDMITPGVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIG 60

Query: 111 NVYVQFREEEQAANALHNLNGRFYAGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNF 170
           NVYVQF+EE+QAA AL  L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNF
Sbjct: 61  NVYVQFKEEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNF 120

Query: 171 MHLKKISRELRRRLFGRSRR-----RRSRSRSRSQSPHKHHGYEER--SHGGGGGRGR-- 230
           MH+K +SRELRR+LFGR RR      RSRSRSRS SP      + R  SH     R R  
Sbjct: 121 MHVKLVSRELRRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDR 180

Query: 231 -----GPSRRDGEKDPRY-HDRSRRPRSRSPRHRGGRSRSPGGRRNRSPVRESSAERRAK 290
                G  +R  E+  R   D SR  R  SP+ RGG   SPGG       RE S ERRA+
Sbjct: 181 EFYRHGSGKRSSERSERQERDGSRGRRQASPK-RGG---SPGGG------REGSEERRAR 240

[Close]

BLAST of CSPI06G25200 vs. TAIR 10
Match: AT1G10320.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-26
Identity = 99/324 (30.56%), Postives = 148/324 (45.68%), Query Frame = 0


Query: 9   FGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDPQGQA 68
           FGTE+D+ +CPF+ K GACR G RCSR+H  P+ S TLL+ NMY  P +       +G  
Sbjct: 237 FGTEQDKAHCPFHLKTGACRFGQRCSRVHFYPNKSCTLLMKNMYNGPGITWE--QDEGLE 296

Query: 69  LDPRKVQDHFEDFYEDLFEELSKYGDLESLNICDNLADHMVGNVYVQFREEEQAANALHN 128
               + +  +E+FYED+  E  KYG+L +  +C N + H+ GNVYV +R  E A  A  +
Sbjct: 297 YTDEEAELCYEEFYEDVHTEFLKYGELVNFKVCRNGSFHLKGNVYVHYRSLESAILAYQS 356

Query: 129 LNGRFYAGRPIIVDFSPVTDFREATCRQYEEN---VCNRGGYCNFMHL------------ 188
           +NGR++AG+ +  +F  ++ ++ A C +Y ++    C+RG  CNF+H             
Sbjct: 357 INGRYFAGKQVNCEFVNISRWKVAICGEYMKSRLKTCSRGSACNFIHCFRNPGGDYEWAD 416

Query: 189 -----KKISRELRRRLFGRSRRRRSRSRSRSQSPHKHHGYEERSHGGGGGRGRGPSRRDG 248
                 +        LFG S  +     S              SH       R PSRR  
Sbjct: 417 HDRPPPRFWIHKMTSLFGYSDEKHMEHESSGSLNDSISDLSTDSH-------RQPSRRSR 476

Query: 249 EKDPRYHDRSRRPRSRSPRHRGGRSRSPGGRRNR-----------SPVRESSAERRA-KI 300
            +D  + +    P  RS ++ G    S    + R           SP R+ S ER   K 
Sbjct: 477 SRDHDHANVGSTPSYRSRKYHGDTQDSTREDKLRRHAENCHDGDDSPSRDGSLEREMYKE 536

The following BLAST results are available for this feature:

BLAST of CSPI06G25200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FMY5	9.4e-106	70.31	Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B ...	[more]
Q9S709	3.0e-104	69.41	Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A ...	[more]
Q6AUG0	2.2e-102	66.01	Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
Q9ZQW8	1.4e-101	66.56	Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
Q9D883	1.1e-69	55.60	Splicing factor U2AF 35 kDa subunit OS=Mus musculus OX=10090 GN=U2af1 PE=1 SV=4	[more]

BLAST of CSPI06G25200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KF96	5.6e-178	99.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G450380 PE=4 SV=1	[more]
A0A1S3CH52	9.3e-173	98.42	splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC10350087...	[more]
A0A5D3C894	9.3e-173	98.42	Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7SM18	9.3e-173	98.42	Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A6J1EGF6	4.9e-166	94.01	splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of CSPI06G25200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143302.1	1.2e-177	99.68	splicing factor U2af small subunit B [Cucumis sativus] >XP_011657685.1 splicing ...	[more]
TYK07378.1	1.9e-172	98.42	enolase [Cucumis melo var. makuwa]	[more]
XP_008462532.1	1.9e-172	98.42	PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >XP_00846253...	[more]
KAA0025291.1	1.9e-172	98.42	splicing factor U2af small subunit B-like [Cucumis melo var. makuwa]	[more]
XP_038882602.1	1.1e-170	96.21	splicing factor U2af small subunit B-like [Benincasa hispida] >XP_038882603.1 sp...	[more]

BLAST of CSPI06G25200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42820.2	6.7e-107	70.31	Zinc finger C-x8-C-x5-C-x3-H type family protein	[more]
AT5G42820.1	6.7e-107	70.31	Zinc finger C-x8-C-x5-C-x3-H type family protein	[more]
AT1G27650.1	2.2e-105	69.41	U2 snRNP auxiliary factor small subunit, putative	[more]
AT1G27650.2	3.4e-74	64.57	U2 snRNP auxiliary factor small subunit, putative	[more]
AT1G10320.1	1.2e-26	30.56	Zinc finger C-x8-C-x5-C-x3-H type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009145	U2 auxiliary factor small subunit	PRINTS	PR01848	U2AUXFACTOR	coord: 37..57 score: 57.14 coord: 18..37 score: 85.68 coord: 130..154 score: 64.0 coord: 103..125 score: 65.42 coord: 165..177 score: 65.73 coord: 75..90 score: 55.97
IPR009145	U2 auxiliary factor small subunit	PANTHER	PTHR12620	U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT	coord: 1..303
IPR003954	RNA recognition motif domain, eukaryote	SMART	SM00361	rrm2_1	coord: 67..142 e-value: 4.4E-7 score: 39.5
IPR000571	Zinc finger, CCCH-type	SMART	SM00356	c3hfinal6	coord: 148..174 e-value: 0.41 score: 19.4 coord: 13..39 e-value: 0.0065 score: 25.7
IPR000571	Zinc finger, CCCH-type	PFAM	PF00642	zf-CCCH	coord: 150..173 e-value: 3.1E-4 score: 20.6 coord: 14..37 e-value: 5.1E-7 score: 29.5
IPR000571	Zinc finger, CCCH-type	PROSITE	PS50103	ZF_C3H1	coord: 12..40 score: 13.112443
IPR000571	Zinc finger, CCCH-type	PROSITE	PS50103	ZF_C3H1	coord: 148..175 score: 10.464717
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 45..142 e-value: 2.2E-4 score: 30.6
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 83..139 e-value: 5.4E-7 score: 29.4
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 44..146 score: 11.622288
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 43..145 e-value: 1.5E-30 score: 107.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 184..201
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 184..317
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 239..256
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 202..238
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 260..293
None	No IPR available	PANTHER	PTHR12620:SF43	RNA RECOGNITION MOTIF DOMAIN, EUKARYOTE-RELATED	coord: 1..303
None	No IPR available	CDD	cd12539	RRM_U2AF35B	coord: 44..145 e-value: 9.85736E-66 score: 199.898
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 77..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G25200.2	CSPI06G25200.2	mRNA
CSPI06G25200.1	CSPI06G25200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
biological_process	GO:0000398	mRNA splicing, via spliceosome
cellular_component	GO:0000015	phosphopyruvate hydratase complex
cellular_component	GO:0005681	spliceosomal complex
cellular_component	GO:0089701	U2AF complex
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0004634	phosphopyruvate hydratase activity
molecular_function	GO:0030628	pre-mRNA 3'-splice site binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003723	RNA binding