CSPI06G24770 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G24770
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Histidine--tRNA ligase
Location	Chr6: 22084657 .. 22090523 (-)
RNA-Seq Expression	CSPI06G24770
Synteny	CSPI06G24770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAGTATAACACAACTAGGCGCCACTTTGCCGCTTCGTTCCATCGTCGACCACCATGGCTGCGAAGCCTTCTTCACTCCTTATCTTCCAACCTCGTCTCAATCTTCGTTTGAAACCCTTCATTTTCTCTTCTTCATCACTCGCTTTCAGTATCATTCTCAGATTCCCCTTTCCTAAACCCTCATTCTTCCTCAACCGCAACTTTCACTCTTCTCTCACCGCCGTCTCTTCCGCTCAGTCTGTTAGCGACGGCGGAACCACTGGCGGCAGATCGGGGGCGCTTTCTCCGGCGCCGACCACTGAAGAATTGCAGAGAATCGATGTTAACCCTCCCAAGGGAACCAGGGATTTTCCACCTGAAGATATGCGACTCCGTAACTGGCTGTTCAATAATTTCAGAGAGGTTGCATATACTGTTGTTTCGCATTGTAGTTTGGTTAGTGATTTTTGCGTCTTTTTTTCATTCGTTTTTAGTTTTTGTCGTTGATTAGGTTTCGCGAGTGTATGGATTTGAGGAGGTTGATTATCCGGTGCTCGAGTCGGAGGCGCTGTTTATAAGGAAAGCTGGTGAAGAAATAAGAGACCAGGTTTCAATCCCTATACTAATTTCTTAAACTAATTTATTGAATTTAGCCCAAAATCTTCATGCTGCAGAAAAAATAAAAGAAAAAGAGAATGAGTAGAACGGCGCCTGTAATTTATTCTTGAACTTATTGAGTATGAGTAATCGGCTGTAATTTCCTGCTTGAATTCAAAAAGAATATTCCTTACGAAAACTATACAAAGAAGAGGGCACTTAATTTATAAAGAACCAAATGAAATCCCAATATCTGATTCTTACATTATTAGATCAAAACAACTAAATGAAAGAAATAATATTCTTTCCCATTTCTGTTTAACGATTTGTGAAGCAAGTCAGTGCAGCATTAAACTAAACTATTGAAGTTATTTATGTTTGGTGCGAGTTTGATAGGGTAGCCGGTAGTATGCCTTGAGAATTCCACATTGGAAAAACAAGGGGACTCATACTCTTTATATGATAGATGGACTGCTCTCATTGCCATTTGGTTTTGAAATGGAACCCATTCTCTAAAATAGTATGAGTTATACTAACAGTGTTTTCCAGCAACGAAATTTTTTGGAATTGAGTTATTAACAAACATTACTAACATGTTATCTTACACTGTCCATCTTTGACTTAAAGCTGACAATTACATGAAATCTAGATTATACAGCATTTTGGAGTTTGTTTTATACATGTTGCAAACTATTTGGGCTTGTATCCTGTGCACATTCATCTTCTTCCTACCTCCACCCTATTCATATTTTGCCTCTTCATAAGGGACATTTTTGGCATTACACTTTATACATGTTTTTCAAATTGTCTTTAATTTGTGCTCTTTCTTTTTGTAGCTCTATTGTTTTGAAGATCGGGGGAATCGTCGAGTTGCTTTGAGGCCTGAGCTTACCCCTTCCTTGGCGAGATTGGTGATCCAGAAAGGGTATTTTTCTTTAAAAGCAACATGTTTCCATTTATCTTGGAACGTGTTTGCTATATTCTACCACTTTTTTTTTGTCTATTTTAGAAGGGAAAAAAATTATAATCACGTGTGTGTAAATTTAATATTTGTCTATTTATTTGTTTAAATTATCTTTGCATCCAGGTACAGATGCCAGATTTTGATCTCCATCTTTTTTTCTGTCCAGAAAATCTTTGTCCCTTCCAATAAAATGGTTTGCCATAGGGCAATGCTGGCGGTATGAGAGAATGACTAGAGGTCGGCGTCGTGAGCATTACCAATGGAATATGGATATCCTTGGTGTACCTGAGGTTACGGTATGAGAATTTTAGTATATGGCATTAGTTTTCTTTTAATTTTTTAAAAATATATGTGTGTGTATTTTATAGCTGAAAGAAGTTTCTGCCTTCTTAGGCAGCAGCTTTGGGCTCTAGATTTTAGTGAAATGAATATAGCTTATGAAGGTTTCTCATAGAAAACAGTTTCTCATAAAAATATCGTGTTTGATGAGTTAGTTTTTTTTTTTTCGAGCACCAATGCAGATGATAGCAAGTACTTGTCAAATGGGATTTTTAGTCATTTTTTCTGTGGATATTTTTGTTAGTCAGACTATCACTCTTCTCAAGTGTTTGTGGGTTCAGAGGATGCAATTTAGTTTAACAAAACAAATATATAACTTGTTTGAAGTCATCTAGCCATCTAGTCATCTTGACATGGTCTGGTACTGGTTGTTATAAAGTTTACTCTTTGGTACCTGGCTTGAATTGAATGTTAAAACTTTTTTGAACAGGGTAGGGTACCTTTTTCTTTGCATACAAATACATATGTGTGCTCATTATCTTCGTTCATTTACTTTTGATCTAATCTTTTTATAGGCTGAAGCAGAGCTGCTATCTTCAATTGTTACGTTTTTCAAGAAAGTTGGAATTACAGCATCGGATGTTGGATTTAAGGTTTCCAGTCGAAAGGTATTTTCTCCGTGCATCTTGTGTGATCATTCCAATAAATGTACTAATGTAGTTATTGAATGTTCAATAGTGAACAAAGATTTTTGTAGTTTCAGTCGCTGTAAATGCCTAATGTTTTTAGTTTGATGTTAGGTTTTGCAAGATGTCCTTAAATGCTATGGAATACCTCAAAATATGTTTGGCAAGGTTTGCATCATCATAGACAAGGTTGGTATCGATTACTCAAGTTCCATTAACTTTTATTTGCTTTTGCAGTGCGTCAGGATGTAATTATTTCTTCTGTCTCTGTTTCCCTCTGGCTCTTAATCTTATTAACAGATAGAGAAAATTCCATTGGATGAGATGAAGAAGGAACTGAAGGTTGTGGGCGTATCAGAGGATGCTATTGAGGAGCTCTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGTTAGAAGGTTATTTTCTGCAAATGGTTAAGTCTTTGACCAATTAGTTGAAAGGGATTCAAATTGGAAGTGTGATTGTTATTCTTGCATAGAGGTACTTGGAGGTGGTGGTGAAGCGTTGTCTGATCTGAAGCAGCTTTTTTCCCTTGCTGAAAAGTTTGGTTATTCTGACTGGATCCAATTTGACGCTTCCATTGTTCGTGGGCTTGCCTATTACACTGGTATTGTTTTTGAGGTTAGTTTTGTTGTTAGTATTGATGTTCACTTTTCCCATGAACTTGTTTGTCTTGTTTATTTGACTTGTCTTCATTGGCATAGGTTTAACATATGTTATATGTCTAACTGAAATAATGCCACGTCCCACACTTTGAACTACAGTGAGCTATATATTCTCATTATAATAAATTAAAGAAATAAAGGGAAAAAAACTACAGCTATTATATCTGTAACTGTTTCCTGAGTTAATTTTATACCAGCATGTAAACGGAATAAATAAAGGTAAATCATCTCATTAAATAGCCACAATCCCCAATTGGAAGTCTAATGCTATAAAGTATATGAAAATTTGTACATCAAATATTTTGTAATATGTTTATGTAATTGCTAGTAAAATCTGATATGTGGCTAAGGAATGGATTTGTAACAGTATATGTTTTCATGATTTTTGTAGTATATTATCAAGCAGAAATGCTGCATTCGCACATAAATAGAGAATAAAATTATATCTTTCATCAAATTTCTTTTTTCCAGAGCATCCATTATTGTTCACTTCTATTTCTTTTCCTTGTTTCTTTGGTGAATATTTCTTCTTAAACTCTCTTCATTTAGTTAAAAGTTTAGTTACATTTATTTCTTGCTTATTGTTTAAGGGAAATAATTGGTAAAGTGATAAAAAGCTCTCTCTCTGTCTTCCCTCTTTTGTCCTTGTATCAGTTAAATTGAAGAAAAGCTAAATGTCATGAAAATTGCTCTTTTGGTTAATTTTAATAATAAAGACGCTGTTTCAGGGCTTTGACAGAGGGGGTAAGCTGCGAGCCATATGCGGTGGAGGTAGGTATGATCGTTTGCTTTCTACATTTGGTGGCGATGATATTCCTGCTTGTGGCTTCGGGTTTGGTGATGCTGTTATTGTAGAGGTAAGTTTTAAGCTGATCTTTGCATCTTTACACAATCATAAAATTCTACGGCACATTATTCCTGTGCTGCAGTTTTCTTACTTCAATTATTAATGTTATGCTTTATGATCAGCTCTGTTTGACTATTTATTTAATAACCAGACCACCTTGAAGTGGCCCATAGTTATGAATCTTATGGTTCAACATGTAGGCCGTTTATCTTATGTATTATATGTAGCACACTAACTTCTTGATATCAAATCTTTTAGGAGTCATTGGTTTTTTGGTAGATTTAGTTGCGTGCATACATTAATGATTAACTCACATAACATGCTTTCGAAAAGTAGGAAAATTTTATTCTCTTTTACTTGGGGAGGTTAATTTTATTCAGAAGTAAACTTCCAATTTTGTCTCATTGAGTTTCAAACAAGGGGTGAAACCAATACATTCTCGAAATTTAGAACATTGAACAGGTTGATGATCACATGGATTGAAATTTCTTGGGCAAAATTCTTGAGGACTTAGGTCAGGAGTTGTGCCTGGATGGTTAAATTTTTTTTACCTAATTATGGAAGTCCTAAAAATAAGATTCTTGCTCCAAGAGGTCTTAAGCTAGGGGAACCTCTCCTTTCTTTTTCTTGGTCGTAGTGGATGTTTTAAGCAAAGTGGTTTTCAAACGTGTTGAAGGGATTTGAAGTGGGTAAGGATAAATTGACTCTCTCCCATCTCCAATGTACCAATTCGCTGATAATATTATATTCTTTTGTTTGGGAAATGACAAACAACCATTTTTGCCGAAACTGCTATGTCCGACCACAAGAACACTCAGAATTAACTTCTAATTAGGTTGAAACCAAGGAAAGCAATGAAACTGAGTTGGTTCCACCCACTGCCAACTGTATCTGTTTGTTGGTTCAAGTTATTTAAATCGTCCGAGGAAAGAACCAAATCATTTAAACCTCTACGCGGAAACCATTAGGGGTTGACCTAGTGGTAAAATCGAAAACATTAATCTAAATACAAGGCTAAGCAGTCATGAGTTCAATCCATGATGACGACCTACCAAAGGATTAACATCTTATGAGTTTTCTTGACACTCAAATATTGTAGTGTTAGACGGGTTGTCTTGTGAGATTAGTGTGCGTAAACTGATTTGGACAAAAGGATTTATTTTAATCACATTCTTCAACAAATGCATACACCTTTTGTCCATACTTCTCTATAGGTAGGATTTGATAAATTTGGTTTTGCTGTTGGGCGTGGGATTTGGTTGTAGTTGCTCAAGGAAAAGAAGTTGCTGCCGGAACTGAATGCTGAAGTAGAGAACATAGTTTGTGCTTTGGATCCTGATCTTCAAGGTGCGGCTTCGAAAGTTGCTACCATTCTCAGGGAGAAAGGCCAAACTGTTGATTTGGTCTTGGAGAACAAACCTCTCAAATGGTATCATTCACAAAACTCAATCGACTCCGTCAAAATTATCACTTTTCTTATCCACACTGCTTTGCTTAATTATTTGTAGGGTGTTCAAACGAGCAACTCGGATAAACGCTCGCCGGTTGATACTAGTTGGAAGTTCAGAATGGGAACGAGGAATGGTCGGAGTTAAAGATCTTTCCTCAGGCGAACAACGTGATGTCAAACTTGATGAACTCGACTAATTTAACCTTCATTCTTTTCTTCTTCTTCTTCTGTAGATTTATTTATTTAGGGAAATAGTTTCAAACTTCAATTTTACATTTTCCATTCTTAGATTATTGTGTATTGAAACGGTTACTGATGGGGATACATCAATGCTATGTTCAATTACAATTTTAGTTTTTGAATTTTGATATTTGTGT

mRNA sequence

TAAGTATAACACAACTAGGCGCCACTTTGCCGCTTCGTTCCATCGTCGACCACCATGGCTGCGAAGCCTTCTTCACTCCTTATCTTCCAACCTCGTCTCAATCTTCGTTTGAAACCCTTCATTTTCTCTTCTTCATCACTCGCTTTCAGTATCATTCTCAGATTCCCCTTTCCTAAACCCTCATTCTTCCTCAACCGCAACTTTCACTCTTCTCTCACCGCCGTCTCTTCCGCTCAGTCTGTTAGCGACGGCGGAACCACTGGCGGCAGATCGGGGGCGCTTTCTCCGGCGCCGACCACTGAAGAATTGCAGAGAATCGATGTTAACCCTCCCAAGGGAACCAGGGATTTTCCACCTGAAGATATGCGACTCCGTAACTGGCTGTTCAATAATTTCAGAGAGGTTTCGCGAGTGTATGGATTTGAGGAGGTTGATTATCCGGTGCTCGAGTCGGAGGCGCTGTTTATAAGGAAAGCTGGTGAAGAAATAAGAGACCAGCTCTATTGTTTTGAAGATCGGGGGAATCGTCGAGTTGCTTTGAGGCCTGAGCTTACCCCTTCCTTGGCGAGATTGGTGATCCAGAAAGGAAAATCTTTGTCCCTTCCAATAAAATGGTTTGCCATAGGGCAATGCTGGCGGTATGAGAGAATGACTAGAGGTCGGCGTCGTGAGCATTACCAATGGAATATGGATATCCTTGGTGTACCTGAGGTTACGGCTGAAGCAGAGCTGCTATCTTCAATTGTTACGTTTTTCAAGAAAGTTGGAATTACAGCATCGGATGTTGGATTTAAGGTTTCCAGTCGAAAGGTTTTGCAAGATGTCCTTAAATGCTATGGAATACCTCAAAATATGTTTGGCAAGGTTTGCATCATCATAGACAAGATAGAGAAAATTCCATTGGATGAGATGAAGAAGGAACTGAAGGTTGTGGGCGTATCAGAGGATGCTATTGAGGAGCTCTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGTTAGAAGAGGTACTTGGAGGTGGTGGTGAAGCGTTGTCTGATCTGAAGCAGCTTTTTTCCCTTGCTGAAAAGTTTGGTTATTCTGACTGGATCCAATTTGACGCTTCCATTGTTCGTGGGCTTGCCTATTACACTGGTATTGTTTTTGAGGGCTTTGACAGAGGGGGTAAGCTGCGAGCCATATGCGGTGGAGGTAGGTATGATCGTTTGCTTTCTACATTTGGTGGCGATGATATTCCTGCTTGTGGCTTCGGGTTTGGTGATGCTGTTATTGTAGAGTTGCTCAAGGAAAAGAAGTTGCTGCCGGAACTGAATGCTGAAGTAGAGAACATAGTTTGTGCTTTGGATCCTGATCTTCAAGGTGCGGCTTCGAAAGTTGCTACCATTCTCAGGGAGAAAGGCCAAACTGTTGATTTGGTCTTGGAGAACAAACCTCTCAAATGGGTGTTCAAACGAGCAACTCGGATAAACGCTCGCCGGTTGATACTAGTTGGAAGTTCAGAATGGGAACGAGGAATGGTCGGAGTTAAAGATCTTTCCTCAGGCGAACAACGTGATGTCAAACTTGATGAACTCGACTAATTTAACCTTCATTCTTTTCTTCTTCTTCTTCTGTAGATTTATTTATTTAGGGAAATAGTTTCAAACTTCAATTTTACATTTTCCATTCTTAGATTATTGTGTATTGAAACGGTTACTGATGGGGATACATCAATGCTATGTTCAATTACAATTTTAGTTTTTGAATTTTGATATTTGTGT

Coding sequence (CDS)

ATGGCTGCGAAGCCTTCTTCACTCCTTATCTTCCAACCTCGTCTCAATCTTCGTTTGAAACCCTTCATTTTCTCTTCTTCATCACTCGCTTTCAGTATCATTCTCAGATTCCCCTTTCCTAAACCCTCATTCTTCCTCAACCGCAACTTTCACTCTTCTCTCACCGCCGTCTCTTCCGCTCAGTCTGTTAGCGACGGCGGAACCACTGGCGGCAGATCGGGGGCGCTTTCTCCGGCGCCGACCACTGAAGAATTGCAGAGAATCGATGTTAACCCTCCCAAGGGAACCAGGGATTTTCCACCTGAAGATATGCGACTCCGTAACTGGCTGTTCAATAATTTCAGAGAGGTTTCGCGAGTGTATGGATTTGAGGAGGTTGATTATCCGGTGCTCGAGTCGGAGGCGCTGTTTATAAGGAAAGCTGGTGAAGAAATAAGAGACCAGCTCTATTGTTTTGAAGATCGGGGGAATCGTCGAGTTGCTTTGAGGCCTGAGCTTACCCCTTCCTTGGCGAGATTGGTGATCCAGAAAGGAAAATCTTTGTCCCTTCCAATAAAATGGTTTGCCATAGGGCAATGCTGGCGGTATGAGAGAATGACTAGAGGTCGGCGTCGTGAGCATTACCAATGGAATATGGATATCCTTGGTGTACCTGAGGTTACGGCTGAAGCAGAGCTGCTATCTTCAATTGTTACGTTTTTCAAGAAAGTTGGAATTACAGCATCGGATGTTGGATTTAAGGTTTCCAGTCGAAAGGTTTTGCAAGATGTCCTTAAATGCTATGGAATACCTCAAAATATGTTTGGCAAGGTTTGCATCATCATAGACAAGATAGAGAAAATTCCATTGGATGAGATGAAGAAGGAACTGAAGGTTGTGGGCGTATCAGAGGATGCTATTGAGGAGCTCTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGTTAGAAGAGGTACTTGGAGGTGGTGGTGAAGCGTTGTCTGATCTGAAGCAGCTTTTTTCCCTTGCTGAAAAGTTTGGTTATTCTGACTGGATCCAATTTGACGCTTCCATTGTTCGTGGGCTTGCCTATTACACTGGTATTGTTTTTGAGGGCTTTGACAGAGGGGGTAAGCTGCGAGCCATATGCGGTGGAGGTAGGTATGATCGTTTGCTTTCTACATTTGGTGGCGATGATATTCCTGCTTGTGGCTTCGGGTTTGGTGATGCTGTTATTGTAGAGTTGCTCAAGGAAAAGAAGTTGCTGCCGGAACTGAATGCTGAAGTAGAGAACATAGTTTGTGCTTTGGATCCTGATCTTCAAGGTGCGGCTTCGAAAGTTGCTACCATTCTCAGGGAGAAAGGCCAAACTGTTGATTTGGTCTTGGAGAACAAACCTCTCAAATGGGTGTTCAAACGAGCAACTCGGATAAACGCTCGCCGGTTGATACTAGTTGGAAGTTCAGAATGGGAACGAGGAATGGTCGGAGTTAAAGATCTTTCCTCAGGCGAACAACGTGATGTCAAACTTGATGAACTCGACTAA

Protein sequence

MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSAQSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNAEVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGSSEWERGMVGVKDLSSGEQRDVKLDELD*

Homology

[Close]

BLAST of CSPI06G24770 vs. ExPASy Swiss-Prot
Match: O82413 (Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g46100 PE=2 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 8.9e-215
Identity = 365/440 (82.95%), Postives = 408/440 (92.73%), Query Frame = 0


Query: 70  GGRSGALSPAPTTEE--LQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVD 129
           GGRSG++     TEE   ++IDVNPPKGTRDFPPEDMRLRNWLFN+F+EVSR+YG+EEVD
Sbjct: 47  GGRSGSIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVD 106

Query: 130 YPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPIKW 189
           YPVLE+EALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS+SLP+KW
Sbjct: 107 YPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKW 166

Query: 190 FAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGITASDVGFK 249
           FAIGQCWRYERMTRGRRREHYQWNMDI+GVP+VTAEAEL+SSIVTFFK++GITASDVGFK
Sbjct: 167 FAIGQCWRYERMTRGRRREHYQWNMDIIGVPQVTAEAELISSIVTFFKRIGITASDVGFK 226

Query: 250 VSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEELLQVL 309
           VSSRKVLQ++LK YG+P+++FG+VCIIIDKIEKIP+DE+KKEL   G+SEDAIE+LLQVL
Sbjct: 227 VSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKELGFTGISEDAIEQLLQVL 286

Query: 310 SVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFD 369
           SVKSL  LE+++GG GEA++DLKQLFSLAEKFGYS+WIQFDAS+VRGLAYYTGIVFEGFD
Sbjct: 287 SVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEGFD 346

Query: 370 RGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNAEVENIVC 429
           R GKLRAICGGGRYDRLLST+GGDD PACGFGFGDAVIVELLKEK LLPEL  EVENIVC
Sbjct: 347 RKGKLRAICGGGRYDRLLSTYGGDDFPACGFGFGDAVIVELLKEKDLLPELGQEVENIVC 406

Query: 430 ALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGSSEWERGM 489
           ALD DLQGAA+ VAT LR+KGQTVDLVLE+KPLKWVFKRA R+NARRL+LVG +EWE G 
Sbjct: 407 ALDKDLQGAAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEWEDGS 466

Query: 490 VGVKDLSSGEQRDVKLDELD 508
           V VK LSSGEQ  VKL +L+
Sbjct: 467 VSVKVLSSGEQFQVKLSDLE 486

[Close]

BLAST of CSPI06G24770 vs. ExPASy Swiss-Prot
Match: Q8U431 (Histidine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=hisS PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-70
Identity = 151/423 (35.70%), Postives = 243/423 (57.45%), Query Frame = 0


Query: 94  KGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDYPVLESEALFIRKAGEEIRDQLYCFE 153
           KGTRDF PE+M  R W+F   RE    YGF+EV  P +E   LF  ++GEE+  QLY F+
Sbjct: 6   KGTRDFLPEEMVKRRWVFERIRERFETYGFKEVLTPTMEYTKLFELRSGEEVVKQLYAFK 65

Query: 154 DRGNRRVALRPELTPSLARLVIQKGKSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNMD 213
           D+G R V+LRP++T S+ARL +   ++   PIKW+ I   +RYE    GR RE +Q  ++
Sbjct: 66  DKGGRDVSLRPDMTSSVARLYVNMFQTAPKPIKWYYIANMFRYEEPQSGRYREFWQAGVE 125

Query: 214 ILGVPEVTAEAELLSSIVTFFKKVGITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCI 273
           ++G  +V A+AE+++  V  +   G+    V   +  R +L +  K  G+  ++   +  
Sbjct: 126 LIGSDKVEADAEVIALFVESYLATGLREFTV--NIGDRILLDEFAKMLGVKDDI--GLMR 185

Query: 274 IIDKIEKIPLDEMKKELKVVGVSEDAIEELLQVLSVK----SLTKLEEVLGGGGEALSDL 333
           IIDK +K+P ++    LK  G+ ++ IE++L+++++K     +  L E L     A +++
Sbjct: 186 IIDKKDKLPQEDFINALKEFGLDKNGIEKVLELINIKGKPDEVLPLAEELFTSEVAKNEI 245

Query: 334 KQLFS---LAEKFGYSDWIQFDASIVRGLAYYTGIVFEGF---DRGGKLRAICGGGRYDR 393
            +L++   L E +   DWI+ D  I RG  YYT IVFE     D G  + +I GGGRYD 
Sbjct: 246 NRLYALIDLLEAYEVKDWIRIDLGIARGFDYYTSIVFEAIAPNDLG--IGSIGGGGRYDN 305

Query: 394 LLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNAEVENIVCALDPDLQGAASKVATI 453
           L+  FGG   PA GF  G   ++ +L+ K L+PE+    +  V  +   ++  A ++AT 
Sbjct: 306 LIEVFGGKPTPATGFAIGIERLIPILEAKGLIPEVQIAPDVYVVPIGERMKKVAIRIATS 365

Query: 454 LREKGQTVDLVLENKPLKWVFKRATRINARRLILVGSSEWERGMVGVKDLSSGEQRDVKL 507
           LR+ G   ++ L  + LK     A ++  ++ I+VG  + ERG+V V+D+ SGEQ +VK+
Sbjct: 366 LRKSGIKTEIELMGRKLKKAMDYANKVGVKKTIIVGEKDLERGVVTVRDMESGEQNEVKI 422

[Close]

BLAST of CSPI06G24770 vs. ExPASy Swiss-Prot
Match: Q9HLX5 (Histidine--tRNA ligase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=hisS PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-70
Identity = 146/425 (34.35%), Postives = 244/425 (57.41%), Query Frame = 0


Query: 85  LQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDYPVLESEALFIRKAGEE 144
           + R+ +   +G RDF PEDM +  ++F    E +  +GF  +D+P LE   L+  K+GEE
Sbjct: 1   MYRLQIEKIRGFRDFYPEDMDVEKFIFKTAEEAAEAFGFRRIDFPSLEYLDLYRIKSGEE 60

Query: 145 IRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPIKWFAIGQCWRYERMTRGRR 204
           +  Q Y F D+G R V L PE TPS  R+V  + K L  P++W++  + WRYE    GR 
Sbjct: 61  LLQQTYSFVDKGGREVTLIPEATPSTVRMVTSR-KDLQRPLRWYSFPKVWRYEEPQAGRY 120

Query: 205 REHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGITASDV-GFKVSSRKVLQDVLKCYGI 264
           REHYQ+N DI G     A+AE+++   +   ++G+   D+   +++SRK++++++   G+
Sbjct: 121 REHYQFNADIFGSDSPEADAEVIALASSILDRLGL--QDIYEIRINSRKIMEEIIG--GM 180

Query: 265 PQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEELLQVLS-VKSLTKLEEVLGGG 324
             +    V  IID+  KI  +E   +L+  G+ ED +  +  + S  + + ++  + G  
Sbjct: 181 TSSDPFSVFSIIDRYHKISREEFVDQLRSAGIGEDGVSMIADLCSGTRGIDEMARITGKS 240

Query: 325 GEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYD 384
            E ++ +  +  L   +G  + +++D SIVRGL+YYTGIVFE +DR G+ RAI GGGRYD
Sbjct: 241 SEEIARMAAVEDLLASYGVKN-VRYDFSIVRGLSYYTGIVFEAYDRSGQFRAILGGGRYD 300

Query: 385 RLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNAEVENI-VCALDPDLQGAASKVA 444
            L S   G+ +PA GFG GDAVI  LLK + +  ++  E +++ +C +        ++ +
Sbjct: 301 NLASLMSGESVPAVGFGMGDAVISLLLKRENV--QIPREKKSVYICRVGKINSSIMNEYS 360

Query: 445 TILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGSSEWERGMVGVKDLSSGEQRDV 504
             LRE+G  V + +  + L    K A+ I A   ++ G  + ERG+V ++++ +G Q +V
Sbjct: 361 RKLRERGMNVTVEIMERGLSAQLKYASAIGADFAVIFGERDLERGVVTIRNMYTGSQENV 417

Query: 505 KLDEL 507
            LD +
Sbjct: 421 GLDSV 417

[Close]

BLAST of CSPI06G24770 vs. ExPASy Swiss-Prot
Match: B1ZUW6 (Histidine--tRNA ligase OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1) OX=452637 GN=hisS PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.1e-70
Identity = 156/423 (36.88%), Postives = 236/423 (55.79%), Query Frame = 0


Query: 95  GTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDYPVLESEALFIRKAGEEIRDQLYCFED 154
           G R+F P+ +  RN +F  +R+ +  +GF E D PVLE   L+  K+G+EI  QL+ F D
Sbjct: 9   GFREFYPDALARRNHIFRLWRQTAVAFGFAEYDAPVLEPLELYKTKSGDEIEAQLFSFTD 68

Query: 155 RGNRRVALRPELTPSLARLVIQKGKSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDI 214
           +G R VALRPE+TP++ R+V  K  +L  PIKWF+I + +RYER  +GR R  +Q+N D+
Sbjct: 69  KGGREVALRPEMTPTVCRMVGAKANALKRPIKWFSIAEYYRYERAQKGRERAFFQFNADL 128

Query: 215 LGVPEVTAEAELLSSIVTFFKKVGITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCII 274
              P   AE EL++ +    K  G+T  D   ++S R +    L+  G+ +     V   
Sbjct: 129 FAEPGPEAEIELIALLTQCLKAFGLTEQDFYIRLSDRNLWFFYLEALGLDEPRIRAVLSA 188

Query: 275 IDKIEKIPLDEMKKELKVVGVSEDAIEE-LLQVLSVKSLTKLEEVLGG-GGEALS----D 334
           +DK EK+  D  K   +  G  + A+++ +L+ L +K+L  LE+ L   GGE L+    D
Sbjct: 189 VDKFEKVGDDAFKPYAEQFGPLDLALKQRVLEFLQIKTLASLEQTLAPLGGEKLAARLGD 248

Query: 335 LKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTF 394
            ++L       G S +I+ D  +VRGLAYYTG VFE FDR G+LRAI GGGRY+ L+   
Sbjct: 249 WRKLLDGLAAMGLSPFIEVDLGVVRGLAYYTGFVFEAFDRKGELRAIAGGGRYNDLVKKL 308

Query: 395 GGDDIPACGFGFGDAVIVELLKEKKLLPELNAEVENIVCALDPDLQGAASKVATI----- 454
           GG D+PA GF  GD  +  LL  + L+P    +  ++ C +     GAA + A       
Sbjct: 309 GGPDLPAVGFAIGDVTLGLLLDARGLMPAF-VQASDVYCVIG----GAAERQAAFADVNA 368

Query: 455 LREKGQTVDLVLENKPLKWVFKRATRINARRLILVGSSEWERGMVGVKDLSSGEQRDVKL 507
           LR  G  VD  L++ P    FK A    A+  ++ G  E  + +V ++DL++  + DV  
Sbjct: 369 LRAAGFKVDYPLKDVPFGKQFKLAADSGAKLALIYGPDELAKNVVKLRDLTTRTETDVPR 426

[Close]

BLAST of CSPI06G24770 vs. ExPASy Swiss-Prot
Match: Q9UY31 (Histidine--tRNA ligase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=hisS PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 6.0e-70
Identity = 157/423 (37.12%), Postives = 237/423 (56.03%), Query Frame = 0


Query: 94  KGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDYPVLESEALFIRKAGEEIRDQLYCFE 153
           KGTRDF PE+M  R W+F   REV   YGF+EV  PV+E   LF  ++GEE+  QLY F+
Sbjct: 6   KGTRDFLPEEMVKRRWVFEKIREVFETYGFKEVLTPVMEYTKLFQLRSGEEVVKQLYAFK 65

Query: 154 DRGNRRVALRPELTPSLARLVIQKGKSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNMD 213
           D+G R VALRP++T S+ARL +   ++   PIKW+ I   +RYE    GR RE +Q  ++
Sbjct: 66  DKGGRDVALRPDMTSSVARLYVNSFQTAPKPIKWYYIANMFRYEEPQSGRYREFWQAGVE 125

Query: 214 ILGVPEVTAEAELLSSIVTFFKKVGITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCI 273
           ++G  ++ A+AE+++  V  +   G+   D    +  R +L +  K  G+  ++   +  
Sbjct: 126 LIGSDKIEADAEVIALFVDSYLSTGL--KDFTVNIGDRVLLDEFAKMLGVKDDI--GLMR 185

Query: 274 IIDKIEKIPLDEMKKELKVVGVSEDAIEELLQVLSVK----SLTKLEEVLGGGGEALSDL 333
           IIDK +K+  +E  K L   G+ E+ IE++L ++ +K     +  L E L     A  ++
Sbjct: 186 IIDKKDKLSQEEFLKALGEFGLDENGIEKVLNLIEIKGKPDDVLPLAEELFTSERAKEEI 245

Query: 334 KQLFSLAEKFGY---SDWIQFDASIVRGLAYYTGIVFEGF---DRGGKLRAICGGGRYDR 393
            +L++L +   +    +WIQ D  I RG  YYT IVFE     D G  + +I GGGRYD 
Sbjct: 246 SRLYNLVDILSWYEVDEWIQIDLGIARGFDYYTSIVFEAIVPNDLG--IGSIGGGGRYDN 305

Query: 394 LLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNAEVENIVCALDPDLQGAASKVATI 453
           L+  FGG   PA GF  G   ++ +L+ K LLPEL A  +  V  +  D +  A+ + T 
Sbjct: 306 LIEVFGGKPTPATGFAIGIERLIPILEWKGLLPELKAGPDVFVIPVG-DSRDVATAIVTR 365

Query: 454 LREKGQTVDLVLENKPLKWVFKRATRINARRLILVGSSEWERGMVGVKDLSSGEQRDVKL 507
           LR+ G   D+ L  + L+     A RI  R  I+VG  + ERG+V ++DL SG Q +V +
Sbjct: 366 LRKAGIRSDIELSGRKLRKALDYANRIGVRLSIIVGKRDLERGVVTIRDLESGNQVEVPV 421

[Close]

BLAST of CSPI06G24770 vs. ExPASy TrEMBL
Match: A0A0A0KER6 (Histidine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_6G446550 PE=3 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.5e-286
Identity = 505/507 (99.61%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60
           MAAKPSSLLIFQP LNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA
Sbjct: 1   MAAKPSSLLIFQPSLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60

Query: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGIT 240
           LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVP VTAEAELLSSIVTFFKKVGIT
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPGVTAEAELLSSIVTFFKKVGIT 240

Query: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

Query: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480
           EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480

Query: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 508
           SEWERGMVGVKDLSSGEQRDVKLDELD
Sbjct: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 507

[Close]

BLAST of CSPI06G24770 vs. ExPASy TrEMBL
Match: A0A5A7VDI2 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold827G00160 PE=3 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 1.0e-282
Identity = 497/507 (98.03%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFF+NR+F+SSLTAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVSSA 60

Query: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS+SDGGTT GRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSR+
Sbjct: 61  QSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRL 120

Query: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGIT 240
           LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFK+VGIT
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVGIT 240

Query: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

Query: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480
           EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480

Query: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 508
           SEWERGMVGVKDLSSGEQ +VKLDEL+
Sbjct: 481 SEWERGMVGVKDLSSGEQCEVKLDELN 507

[Close]

BLAST of CSPI06G24770 vs. ExPASy TrEMBL
Match: A0A1S3CHK2 (Histidine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103500830 PE=3 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 1.0e-282
Identity = 497/507 (98.03%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFF+NR+F+SSLTAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVSSA 60

Query: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS+SDGGTT GRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSR+
Sbjct: 61  QSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRL 120

Query: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGIT 240
           LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFK+VGIT
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVGIT 240

Query: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

Query: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480
           EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480

Query: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 508
           SEWERGMVGVKDLSSGEQ +VKLDEL+
Sbjct: 481 SEWERGMVGVKDLSSGEQCEVKLDELN 507

[Close]

BLAST of CSPI06G24770 vs. ExPASy TrEMBL
Match: A0A5D3C5Z0 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00380 PE=3 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.3e-282
Identity = 496/507 (97.83%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFF+NR+F+SSLTAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVSSA 60

Query: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS+SDGGTT GRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSR+
Sbjct: 61  QSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRL 120

Query: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGIT 240
           LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFK+VGIT
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVGIT 240

Query: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

Query: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480
           EVENIVCALDPDLQGAASKVAT+LREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASKVATVLREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480

Query: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 508
           SEWERGMVGVKDLSSGEQ +VKLDEL+
Sbjct: 481 SEWERGMVGVKDLSSGEQCEVKLDELN 507

[Close]

BLAST of CSPI06G24770 vs. ExPASy TrEMBL
Match: A0A6J1IL10 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.7e-272
Identity = 472/507 (93.10%), Postives = 496/507 (97.83%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSS+SLAFSIILR PFPKPSFF NR FHSSL+AVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSTSLAFSIILRIPFPKPSFFGNRKFHSSLSAVSSA 60

Query: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS+ DGG  GGRSGALSP   TEELQ+IDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSIGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVD+PVLESEALFIRKAGEEIR+QLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIREQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGIT 240
           LSLP+KWFAIGQCWRYERMTRGRRREHYQWNMDI+GVPEVTAEAELLSSIV FFK++G+T
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           A+DVGFKVSSRKVLQ+VLKCYGIP+NMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EE+LQVLSVKSLTKLEE+LGGGGEA+SDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDD+PACGFGFGDAVIVELLKEKKLLPEL+ 
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELSV 420

Query: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480
           EVENIVCALDPDLQGAAS+VATILREKGQTVDLVLENKPLKWVFKRATRINA+RLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480

Query: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 508
           SEW+RGMVGVKDLSSGEQR+VKLDEL+
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of CSPI06G24770 vs. NCBI nr
Match: XP_004143277.1 (histidine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN48195.1 hypothetical protein Csa_002968 [Cucumis sativus])

HSP 1 Score: 994.6 bits (2570), Expect = 3.2e-286
Identity = 505/507 (99.61%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60
           MAAKPSSLLIFQP LNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA
Sbjct: 1   MAAKPSSLLIFQPSLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60

Query: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGIT 240
           LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVP VTAEAELLSSIVTFFKKVGIT
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPGVTAEAELLSSIVTFFKKVGIT 240

Query: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

Query: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480
           EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480

Query: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 508
           SEWERGMVGVKDLSSGEQRDVKLDELD
Sbjct: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 507

[Close]

BLAST of CSPI06G24770 vs. NCBI nr
Match: XP_008462490.1 (PREDICTED: histidine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo] >KAA0063801.1 histidine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 981.9 bits (2537), Expect = 2.1e-282
Identity = 497/507 (98.03%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFF+NR+F+SSLTAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVSSA 60

Query: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS+SDGGTT GRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSR+
Sbjct: 61  QSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRL 120

Query: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGIT 240
           LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFK+VGIT
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVGIT 240

Query: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

Query: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480
           EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480

Query: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 508
           SEWERGMVGVKDLSSGEQ +VKLDEL+
Sbjct: 481 SEWERGMVGVKDLSSGEQCEVKLDELN 507

[Close]

BLAST of CSPI06G24770 vs. NCBI nr
Match: TYK07323.1 (histidine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 981.5 bits (2536), Expect = 2.8e-282
Identity = 496/507 (97.83%), Postives = 505/507 (99.61%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFF+NR+F+SSLTAVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVSSA 60

Query: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS+SDGGTT GRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSR+
Sbjct: 61  QSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRL 120

Query: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGIT 240
           LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFK+VGIT
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVGIT 240

Query: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

Query: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480
           EVENIVCALDPDLQGAASKVAT+LREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASKVATVLREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480

Query: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 508
           SEWERGMVGVKDLSSGEQ +VKLDEL+
Sbjct: 481 SEWERGMVGVKDLSSGEQCEVKLDELN 507

[Close]

BLAST of CSPI06G24770 vs. NCBI nr
Match: XP_038883346.1 (histidine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 973.4 bits (2515), Expect = 7.6e-280
Identity = 486/507 (95.86%), Postives = 503/507 (99.21%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60
           MA KPSSLLIFQPRLNLRLKPFIFSSSSLAFSIIL+FPFPKPSFF+NRNFHSSLTAVSSA
Sbjct: 1   MATKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILKFPFPKPSFFVNRNFHSSLTAVSSA 60

Query: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS++DGGT+GGRSGALSPAP TEELQ+IDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSITDGGTSGGRSGALSPAPVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVDYPVLESEALF+RKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDYPVLESEALFVRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGIT 240
           LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDI+GVPEVTAEAELLSSIVTFFK+VGIT
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVTFFKRVGIT 240

Query: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           ASDVGFKVSSRKVLQDVLKCYGIP+NMFGKVCIIIDKIEKIPLDE+KKELKVVG+SEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPENMFGKVCIIIDKIEKIPLDEIKKELKVVGISEDAI 300

Query: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420
           IVFEGFDRGGKLRAICGGGRYD LLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDHLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

Query: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480
           EVENI+CALDPDLQGAAS VATILREKGQ+VDLVLENKPLKWVFKRATRINARRLILVGS
Sbjct: 421 EVENIICALDPDLQGAASTVATILREKGQSVDLVLENKPLKWVFKRATRINARRLILVGS 480

Query: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 508
           SEW+RGMVGVKDLSSGEQR+VKLDEL+
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of CSPI06G24770 vs. NCBI nr
Match: XP_022978407.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 948.0 bits (2449), Expect = 3.4e-272
Identity = 472/507 (93.10%), Postives = 496/507 (97.83%), Query Frame = 0


Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60
           MAAKPSSLLIFQPRLNLRLKPFIFSS+SLAFSIILR PFPKPSFF NR FHSSL+AVSSA
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSTSLAFSIILRIPFPKPSFFGNRKFHSSLSAVSSA 60

Query: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120
           QS+ DGG  GGRSGALSP   TEELQ+IDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV
Sbjct: 61  QSIGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180
           YGFEEVD+PVLESEALFIRKAGEEIR+QLYCFEDRGNRRVALRPELTPSLARLVIQKGKS
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIREQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGIT 240
           LSLP+KWFAIGQCWRYERMTRGRRREHYQWNMDI+GVPEVTAEAELLSSIV FFK++G+T
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           A+DVGFKVSSRKVLQ+VLKCYGIP+NMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EE+LQVLSVKSLTKLEE+LGGGGEA+SDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDD+PACGFGFGDAVIVELLKEKKLLPEL+ 
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELSV 420

Query: 421 EVENIVCALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGS 480
           EVENIVCALDPDLQGAAS+VATILREKGQTVDLVLENKPLKWVFKRATRINA+RLILVGS
Sbjct: 421 EVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRATRINAQRLILVGS 480

Query: 481 SEWERGMVGVKDLSSGEQRDVKLDELD 508
           SEW+RGMVGVKDLSSGEQR+VKLDEL+
Sbjct: 481 SEWQRGMVGVKDLSSGEQREVKLDELN 507

[Close]

BLAST of CSPI06G24770 vs. TAIR 10
Match: AT3G46100.1 (Histidyl-tRNA synthetase 1 )

HSP 1 Score: 747.7 bits (1929), Expect = 6.3e-216
Identity = 365/440 (82.95%), Postives = 408/440 (92.73%), Query Frame = 0


Query: 70  GGRSGALSPAPTTEE--LQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVD 129
           GGRSG++     TEE   ++IDVNPPKGTRDFPPEDMRLRNWLFN+F+EVSR+YG+EEVD
Sbjct: 47  GGRSGSIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVD 106

Query: 130 YPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPIKW 189
           YPVLE+EALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS+SLP+KW
Sbjct: 107 YPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKW 166

Query: 190 FAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGITASDVGFK 249
           FAIGQCWRYERMTRGRRREHYQWNMDI+GVP+VTAEAEL+SSIVTFFK++GITASDVGFK
Sbjct: 167 FAIGQCWRYERMTRGRRREHYQWNMDIIGVPQVTAEAELISSIVTFFKRIGITASDVGFK 226

Query: 250 VSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEELLQVL 309
           VSSRKVLQ++LK YG+P+++FG+VCIIIDKIEKIP+DE+KKEL   G+SEDAIE+LLQVL
Sbjct: 227 VSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKELGFTGISEDAIEQLLQVL 286

Query: 310 SVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFD 369
           SVKSL  LE+++GG GEA++DLKQLFSLAEKFGYS+WIQFDAS+VRGLAYYTGIVFEGFD
Sbjct: 287 SVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEGFD 346

Query: 370 RGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNAEVENIVC 429
           R GKLRAICGGGRYDRLLST+GGDD PACGFGFGDAVIVELLKEK LLPEL  EVENIVC
Sbjct: 347 RKGKLRAICGGGRYDRLLSTYGGDDFPACGFGFGDAVIVELLKEKDLLPELGQEVENIVC 406

Query: 430 ALDPDLQGAASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGSSEWERGM 489
           ALD DLQGAA+ VAT LR+KGQTVDLVLE+KPLKWVFKRA R+NARRL+LVG +EWE G 
Sbjct: 407 ALDKDLQGAAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEWEDGS 466

Query: 490 VGVKDLSSGEQRDVKLDELD 508
           V VK LSSGEQ  VKL +L+
Sbjct: 467 VSVKVLSSGEQFQVKLSDLE 486

[Close]

BLAST of CSPI06G24770 vs. TAIR 10
Match: AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 376.7 bits (966), Expect = 2.9e-104
Identity = 183/259 (70.66%), Postives = 217/259 (83.78%), Query Frame = 0


Query: 26  SSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSAQSVSDGGTTGGRSGALSPAPTTEEL 85
           SSS    ++L F    P     R F +    + +A  V+ GG +G     ++P    EE 
Sbjct: 12  SSSFIRPVLLDFVSCSPL----RQFSTPRRFICAASKVNGGGRSG---SIVAPLVVNEEF 71

Query: 86  QRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDYPVLESEALFIRKAGEEI 145
           Q IDVNPPKGTRDFPPEDMRLRNWLFN+F+EVSR++G+EEVD+PVLE+EALFIRKAGEEI
Sbjct: 72  QNIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEVDFPVLETEALFIRKAGEEI 131

Query: 146 RDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSLSLPIKWFAIGQCWRYERMTRGRRR 205
           RDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS+SLP+KWFA+GQCWRYERMTRGRRR
Sbjct: 132 RDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAVGQCWRYERMTRGRRR 191

Query: 206 EHYQWNMDILGVPEVTAEAELLSSIVTFFKKVGITASDVGFKVSSRKVLQDVLKCYGIPQ 265
           EHYQWNMDI+GVPEVTAEAEL+SSI+TFFK++GITASDVGFKVSSRKVLQ+ LK YG+P+
Sbjct: 192 EHYQWNMDIIGVPEVTAEAELISSIMTFFKRIGITASDVGFKVSSRKVLQEFLKKYGVPE 251

Query: 266 NMFGKVCIIIDKIEKIPLD 285
           ++FG+VC IIDK+  + ++
Sbjct: 252 DLFGRVCFIIDKVSNLNIN 263

[Close]

BLAST of CSPI06G24770 vs. TAIR 10
Match: AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 165.6 bits (418), Expect = 1.0e-40
Identity = 131/423 (30.97%), Postives = 207/423 (48.94%), Query Frame = 0


Query: 93  PKGTRDFPPEDMRLRNWLFNNFREVSRVYGFEEVDYPVLESEALFIRKAGEEIRDQLYCF 152
           PKGTRDF  E M +R   F+  + V + +G   +D PV E     + K GE+ +  +Y  
Sbjct: 449 PKGTRDFAKEQMAVREKAFSIIQNVFKRHGATALDTPVFELRETLMGKYGEDSK-LVYDI 508

Query: 153 EDRGNRRVALRPELTPSLARLVIQKGKSLSLPIKWFAIGQCWRYERMTRGRRREHYQWNM 212
            D+G    +LR +LT   AR V   G +     K + I + +R +  ++GR RE YQ + 
Sbjct: 509 ADQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRYREFYQCDF 568

Query: 213 DILGVPE-VTAEAELLSSIVTFFKKVGITASDVGFKVSSRKVLQDVLKCYGIPQNMFGKV 272
           DI G+ E +  + E++  +     ++ I   D   K++ RK+L  +L+  G+P   F  +
Sbjct: 569 DIAGLFEPMGPDFEIVKILTELLDELEI--GDYEVKLNHRKLLDGMLEICGVPPEKFRTI 628

Query: 273 CIIIDKIEKIPLDEMKKEL-KVVGVSEDAIEELLQVLSVKS-----LTKL----EEVLG- 332
           C  IDK++K   +++KKE+ +  G+S +  + +   +  K      L+KL     E L  
Sbjct: 629 CSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIGNFVKEKGAPLELLSKLRQEGSEFLDN 688

Query: 333 -GGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGG 392
               EAL +L  +F   ++   S+ I FD S+ RGL YYTG++FE    G ++ +I  GG
Sbjct: 689 QSSREALDELSIMFEALKRSKCSERIVFDLSLARGLDYYTGVIFEAVCIGAEVGSIGAGG 748

Query: 393 RYDRLLSTFGGDDIPACGFGFG-DAV--IVELLKEKKLLPELNAEVENIVCAL-DPDLQG 452
           RYD L+  FG   +PA G   G + V  I+E L EK+       E + +V  + D  L  
Sbjct: 749 RYDNLIGMFGTKQVPAVGMSLGIERVFNIMEELNEKQKQVIRPTETQVLVSIMVDNKLAE 808

Query: 453 AASKVATILREKGQTVDLVLENKPLKWVFKRATRINARRLILVGSSEWERGMVGVKDLSS 499
           AA  V+ +   K     LV + K     F RA       +++VG  E     V +K L  
Sbjct: 809 AAELVSQLWGAKINAEYLVSKRKEKH--FNRAKESGIPWMVMVGEKELSGSFVTLKKLEK 863

The following BLAST results are available for this feature:

BLAST of CSPI06G24770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82413	8.9e-215	82.95	Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3...	[more]
Q8U431	1.2e-70	35.70	Histidine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JC...	[more]
Q9HLX5	1.2e-70	34.35	Histidine--tRNA ligase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728...	[more]
B1ZUW6	2.1e-70	36.88	Histidine--tRNA ligase OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1...	[more]
Q9UY31	6.0e-70	37.12	Histidine--tRNA ligase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=hi...	[more]

BLAST of CSPI06G24770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KER6	1.5e-286	99.61	Histidine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_6G446550 PE=3 SV=1	[more]
A0A5A7VDI2	1.0e-282	98.03	Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8...	[more]
A0A1S3CHK2	1.0e-282	98.03	Histidine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103500830 PE=3 SV=1	[more]
A0A5D3C5Z0	1.3e-282	97.83	Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2...	[more]
A0A6J1IL10	1.7e-272	93.10	Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111478405 PE=3 SV=1	[more]

BLAST of CSPI06G24770 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143277.1	3.2e-286	99.61	histidine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN48195....	[more]
XP_008462490.1	2.1e-282	98.03	PREDICTED: histidine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo] >K...	[more]
TYK07323.1	2.8e-282	97.83	histidine--tRNA ligase [Cucumis melo var. makuwa]	[more]
XP_038883346.1	7.6e-280	95.86	histidine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida]	[more]
XP_022978407.1	3.4e-272	93.10	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima...	[more]

BLAST of CSPI06G24770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46100.1	6.3e-216	82.95	Histidyl-tRNA synthetase 1	[more]
AT5G03406.1	2.9e-104	70.66	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G02760.1	1.0e-40	30.97	Class II aaRS and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain	PFAM	PF13393	tRNA-synt_His	coord: 95..399 e-value: 1.3E-46 score: 159.3
IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain	CDD	cd00773	HisRS-like_core	coord: 103..410 e-value: 1.20162E-97 score: 293.74
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 87..414 e-value: 2.3E-115 score: 387.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 61..99
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 61..80
None	No IPR available	PANTHER	PTHR43707:SF1	HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED	coord: 86..507
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 92..411
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 420..506
IPR004516	Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit	PIRSF	PIRSF001549	His-tRNA_synth	coord: 86..507 e-value: 1.3E-146 score: 486.6
IPR004516	Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit	PANTHER	PTHR43707	HISTIDYL-TRNA SYNTHETASE	coord: 86..507
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 428..506 e-value: 7.4E-10 score: 38.9
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 415..507 e-value: 5.6E-20 score: 72.8
IPR015807	Histidine-tRNA ligase	TIGRFAM	TIGR00442	TIGR00442	coord: 92..502 e-value: 8.5E-105 score: 349.0
IPR015807	Histidine-tRNA ligase	HAMAP	MF_00127	His_tRNA_synth	coord: 88..507 score: 25.357656
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 95..416 score: 12.789536

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G24770.1	CSPI06G24770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006427	histidyl-tRNA aminoacylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004821	histidine-tRNA ligase activity