CSPI06G21370 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G21370
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionmRNA-decapping enzyme subunit 2-like
LocationChr6: 19331062 .. 19335485 (-)
RNA-Seq ExpressionCSPI06G21370
SyntenyCSPI06G21370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATATTCATAGAATTGAGTCAAATGAGCATATCGGGTTTTATCGTTTCGCGGCTGCTACACTTGTTGGTGTGTGTTCGGGTGAGGGTCGAAGAAAAAGAGCTGAAAATCTTCATCCTCCTTTCTTCCCGATTTCCAATTCTCCGAACTCCATGGCCGCTCTTCACCACTCCTCTTCCACTACGCCCGTCAAAAACGGCCTTCCTCCTCAAGAACTCATCGACGATCTTTGCAGGTCTCTTCTTCAACTCAACTCAACTCTCACTTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTCTTCTTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAACTCCTCCATAATGTACTTCCATCATCTCATTCCTTTCATCTTCACTGTCTTCTTTTTTCTTTTTTCTTTTTTCTTCAATGTCTTTTCACATTCTGTTGATTACTGTTTTGCAACGCCATCGATCGTAATTGAGAGAACTTGTTAAAGAAGAGTAGTCAGTTTACTTGCTTAAAGTTTCCCTTAAAAACGGCGATGAAACTTAGGGAGTTGAAACTTGGAATTTGAATGCGGTTTATGAGAAAGGAACATAGTGGATCGGCTACACGGATGTTCCTTCTATGTATGTGTATGTGTATGTGTATTTGTTATTGTTATTGTTTTGAGTGATATCTCTACTTACTTAACTTGAACATGATTGATGTCGAACAATTTCTGTTTATGAATTATGGTAGGGAGTATTGTTTGTTCATACTTTTGTTAAGAGGAGTTTGTTGGCACGTTACTGGGTGTGCTGTTGGATTGTTTTTCCTTTCTTTCTTTCTGGAAGCAACGTTATGTTCTTCAATGTAGCAACAGGCTAAGAAATTTTGGCCTTGAACATTGATGACTGATAATGTTATTGGATGTCAAAGGACGTTTTATGCAAAATAAAAGAAGCTCTGCTTGATTGATTTTTTGGAGGATCTTTTAGAATACAGTTTCCGATACTGATTTAGTAAGAAATTTCACAGGTATTTATCAACAGATGGACTAGAATTTTTATTGGAAACTTAGTTGTTACTCGAATAAAAGGTTGTTTTTTCTTGGACCTAGATCCATTTCATCCATAGTCTTTGTGTGAGATTTGACCTTGATGGATCTTAGATTCATTCTTCGTTATTTTCCTTATTTTCTAACCTGCTTGATGCAGCATTGAATCTCTTTTCCTCTTTCTTTATTTTGTTAAATATTTGTCTTGCTTTTTTTCTGCAGTCGTTTTGTTTTGAATGTGCCGAAAGAAGATCTACAGTCATTTGAGAGGATTCTATTCCTCATAGAGTATGCACATTGGTTTTATGAAGACAATTCTGTGGAAAGAAATCCATCTTTAAAGTCATTGAATCTGAAGGAATTCACTTCATTAAGTATCCTGCACTGTTTTGCAACCATTTGATTATGGAAAAATAATTCGGAGTTCTCTTTCTATATATCTCTCTCAAATGATGACTAATAATTGTTAGTGGTATACTTTATATCAGTGTTTGCACATTCCTGTTTTCCCTTACTATATATCAGTGTTTAAAAGCTGTGATGTTTTAAGACCATATGTTGCCCATATTGATGATATCTTTAAAGACTTCACTTCATACAAGGTTCGAGTTCCTGTAACTGGGGCAATCATACTGGATGAGACATTTGAGCGGGTGAGGTCTTGTTAATGAAACTTGGCTCACTTAGCCCCCTCCCCACCCTCGCATTATTAATGCAAAGGATGTTGACAATGCTCCCTTTTGCTTATTATTGGCAAAATACAGTGCTTGTTAGTGAAGGGATGGAAAGGATCTAGTTGGAGTTTTCCACGCGGTAAAAAGAGCAAAGATGAGGAAGACCATGCTTGTGCCATTAGAGAAGTAAGCTTGATTCTTTCCGAATTTTCTCATTGCATAGTCAGCATTCTTGTGTGTCATGTAAATACCACTCTGATTGTAACTGCAAATTTCACTTGAAGTTTAATGAATTAGAAGGTGTCAGATGTTATTCCATCTTCTTCTATAGGATCTGGCCAAGGAATGAATTTTGGTTCAATAATGTTCCATATATGGGGGGGAAGTTTTTCAGTTACCGGGAAAATTTTACAAACATTGATTTCCTGTTGACAGACAGTACCGGGTCTTCTATAAATTTGAGATGTACCATTTGATTGCAGGTCCTGGAAGAAACAGGATTTGACATTAGTAATCTTCTCAAAAAATCTGAATACATAGAAGTTATATTTGGACCGCAGAGGGTGCGGCTTTACATCATTGCTGGCGTGAAAGATGATAATCTCTTTGCCCCGCAAACTAAGAAAGAGATCAGTGTATGTGTGTTTCCTTACCTCAAAACATTTTTCTAGAGTTTTATTTTTGATTTGCATTGAAATCACTACCTCAAAATATGTTTTTTAAAGTTTAGTTCTTCAGGTTTTTTGCCTGCCATTCTTTTAATATTTTAAGCATTTATTATCCTACTCGAACTCCTAAAATTGTCTTGGAGCCAACCTGAGCTTTGTTTAACCAGGTTAAGTGTTTTATGCCACCCTTGAAGGATCACAAGTTAAAATCTTTTCCTGTATTTGTAACATAATTCTTCTTCTTAAAAAAAAAAACAAAAAAAGTCTCATTCCTTTGAAGGTTTTGGTAATTATGAACTATCATCTGATTATGGGCCATGCCTATCAAATGTTGAAAAGGTAGGTGTGTAGCTTTTAATTTTGAGCCCAGAGAAAATGGGTTCAACAAATTTCTGGGTAGAACGTAAAAGAACAGGATTATTTATTAATAACAGGTCATCATTGGAACTTTCTATAGTTGAAAGCACGTACTTAACTATTTGTAAACAGGAAATTGCATGGCATCGACTCGATGATCTTGAACCAGCTAGTGATAATGTAATATCTCGTGGGATGAGCGGTCTCAAGCTTTATATGATTGCTCCTTTTTTGGCGTGAGTACACCTGTATATTCTTAATGATTTCTTCTTCATGCTTTAACAAATTAGATATCTTAAGGGGTCGGCCTTTTCGTGCAGATCTCTAAGGTCATGGATTTTGACACATCATCCCCCTGTTCGTCCAAACCCCGATATCCCAATAAGAGGTTTTCTTTTCTCAGCTTACTTAGAGATAATTAGATTTTGCTGTTATTTTCAAAGATAAGACTCTGTATTTCAGTTCAAGTTAATAGATTGTCCAAATTCCAATGCTTAATCTCACAAACTTACATTCATTAATTTACATATTTAGGGTTTCTGAAATATGCTAATGTAGTGTCTGTTCATGTTTTCAAGGACTAACGGTATGGAAGGCTAAGAACAGCTCGATAGGTAGCAGCAGTACAATCATAATGGAGAGCCAAATAAGTAAAGCAGAAGTGGAAGTTGACACTGGTCCAGGCAAGAGCTTCAGAAATTTCAAGTTTGATACTGCCTCAATCTTACAATCAATGGAAAGAGCCTTTTCTTAAGTCTTCTATAAGTCCTAAATTCCTTTTTTTTCTTAATTAGCTCATGCTTAGACATTATAGATATGTCAGATTACTAACTCATAGTAACAGCAGAAGAAAAAAAAAAGTAGAAATAGGAGTGATAGCATAGATAGAGAGGGAGGGAAAGTGATTTCATCTACCCAACCTTCCTCCTCCTCGCTATTTTGGAAATAAAAAGTGTTGGTTTAGAGTTTTGATTTTGTGTGAAATTTGCATATAGTCTGTAGTCTATACAAAAGTATCGGTGTGAAAATGTTTGTAAAGAAAAGAAAAAGGCTCCAAGAATTGGCATGAATTTGGAGTGTTCTTGTGGTGAAAGAGAGAGATGGAGAAATCACTTCTTGATGTACTTTGCCAGCTTGATTGCAACGACAGGTATCAGTTCGTAGGAGTTTATGTTTATGGCAAATGGGGTTTTCTGTACAGAAACATTGATGTGAGAAATGTGTTGAACTTTGATGTTTACTTTAGTTTTTATCGGTTTGGTTTGGTATTCTTGTGGGGGTGAGCACTTTTAGAAATGCTTCTCCTCTTCCCTCCTCTTTCCCAGTGTGGCTCGGCTTCTACCCATTTCTTTGTTGTTTCACTGAAACTTTGCTAGTTATGTGTTTTTTCCCCTTTATATCTATGCGATGTGTGTGTGCG

mRNA sequence

ATGGATATTCATAGAATTGAGTCAAATGAGCATATCGGGTTTTATCGTTTCGCGGCTGCTACACTTGTTGGTGTGTGTTCGGGTGAGGGTCGAAGAAAAAGAGCTGAAAATCTTCATCCTCCTTTCTTCCCGATTTCCAATTCTCCGAACTCCATGGCCGCTCTTCACCACTCCTCTTCCACTACGCCCGTCAAAAACGGCCTTCCTCCTCAAGAACTCATCGACGATCTTTGCAGTCGTTTTGTTTTGAATGTGCCGAAAGAAGATCTACAGTCATTTGAGAGGATTCTATTCCTCATAGAGTATGCACATTGGTTTTATGAAGACAATTCTGTGGAAAGAAATCCATCTTTAAAGTCATTGAATCTGAAGGAATTCACTTCATTAATGTTTAAAAGCTGTGATGTTTTAAGACCATATGTTGCCCATATTGATGATATCTTTAAAGACTTCACTTCATACAAGGTTCGAGTTCCTGTAACTGGGGCAATCATACTGGATGAGACATTTGAGCGGTGCTTGTTAGTGAAGGGATGGAAAGGATCTAGTTGGAGTTTTCCACGCGGTAAAAAGAGCAAAGATGAGGAAGACCATGCTTGTGCCATTAGAGAAGTCCTGGAAGAAACAGGATTTGACATTAGTAATCTTCTCAAAAAATCTGAATACATAGAAGTTATATTTGGACCGCAGAGGGTGCGGCTTTACATCATTGCTGGCGTGAAAGATGATAATCTCTTTGCCCCGCAAACTAAGAAAGAGATCAGTGAAATTGCATGGCATCGACTCGATGATCTTGAACCAGCTAGTGATAATGTAATATCTCGTGGGATGAGCGGTCTCAAGCTTTATATGATTGCTCCTTTTTTGGCATCTCTAAGGTCATGGATTTTGACACATCATCCCCCTGTTCGTCCAAACCCCGATATCCCAATAAGAGGACTAACGGTATGGAAGGCTAAGAACAGCTCGATAGGTAGCAGCAGTACAATCATAATGGAGAGCCAAATAAGTAAAGCAGAAGTGGAAGTTGACACTGGTCCAGGCAAGAGCTTCAGAAATTTCAAGTTTGATACTGCCTCAATCTTACAATCAATGGAAAGAGCCTTTTCTTAAGTCTTCTATAAGTCCTAAATTCCTTTTTTTTCTTAATTAGCTCATGCTTAGACATTATAGATATGTCAGATTACTAACTCATAGTAACAGCAGAAGAAAAAAAAAAGTAGAAATAGGAGTGATAGCATAGATAGAGAGGGAGGGAAAGTGATTTCATCTACCCAACCTTCCTCCTCCTCGCTATTTTGGAAATAAAAAGTGTTGGTTTAGAGTTTTGATTTTGTGTGAAATTTGCATATAGTCTGTAGTCTATACAAAAGTATCGGTGTGAAAATGTTTGTAAAGAAAAGAAAAAGGCTCCAAGAATTGGCATGAATTTGGAGTGTTCTTGTGGTGAAAGAGAGAGATGGAGAAATCACTTCTTGATGTACTTTGCCAGCTTGATTGCAACGACAGGTATCAGTTCGTAGGAGTTTATGTTTATGGCAAATGGGGTTTTCTGTACAGAAACATTGATGTGAGAAATGTGTTGAACTTTGATGTTTACTTTAGTTTTTATCGGTTTGGTTTGGTATTCTTGTGGGGGTGAGCACTTTTAGAAATGCTTCTCCTCTTCCCTCCTCTTTCCCAGTGTGGCTCGGCTTCTACCCATTTCTTTGTTGTTTCACTGAAACTTTGCTAGTTATGTGTTTTTTCCCCTTTATATCTATGCGATGTGTGTGTGCG

Coding sequence (CDS)

ATGGATATTCATAGAATTGAGTCAAATGAGCATATCGGGTTTTATCGTTTCGCGGCTGCTACACTTGTTGGTGTGTGTTCGGGTGAGGGTCGAAGAAAAAGAGCTGAAAATCTTCATCCTCCTTTCTTCCCGATTTCCAATTCTCCGAACTCCATGGCCGCTCTTCACCACTCCTCTTCCACTACGCCCGTCAAAAACGGCCTTCCTCCTCAAGAACTCATCGACGATCTTTGCAGTCGTTTTGTTTTGAATGTGCCGAAAGAAGATCTACAGTCATTTGAGAGGATTCTATTCCTCATAGAGTATGCACATTGGTTTTATGAAGACAATTCTGTGGAAAGAAATCCATCTTTAAAGTCATTGAATCTGAAGGAATTCACTTCATTAATGTTTAAAAGCTGTGATGTTTTAAGACCATATGTTGCCCATATTGATGATATCTTTAAAGACTTCACTTCATACAAGGTTCGAGTTCCTGTAACTGGGGCAATCATACTGGATGAGACATTTGAGCGGTGCTTGTTAGTGAAGGGATGGAAAGGATCTAGTTGGAGTTTTCCACGCGGTAAAAAGAGCAAAGATGAGGAAGACCATGCTTGTGCCATTAGAGAAGTCCTGGAAGAAACAGGATTTGACATTAGTAATCTTCTCAAAAAATCTGAATACATAGAAGTTATATTTGGACCGCAGAGGGTGCGGCTTTACATCATTGCTGGCGTGAAAGATGATAATCTCTTTGCCCCGCAAACTAAGAAAGAGATCAGTGAAATTGCATGGCATCGACTCGATGATCTTGAACCAGCTAGTGATAATGTAATATCTCGTGGGATGAGCGGTCTCAAGCTTTATATGATTGCTCCTTTTTTGGCATCTCTAAGGTCATGGATTTTGACACATCATCCCCCTGTTCGTCCAAACCCCGATATCCCAATAAGAGGACTAACGGTATGGAAGGCTAAGAACAGCTCGATAGGTAGCAGCAGTACAATCATAATGGAGAGCCAAATAAGTAAAGCAGAAGTGGAAGTTGACACTGGTCCAGGCAAGAGCTTCAGAAATTTCAAGTTTGATACTGCCTCAATCTTACAATCAATGGAAAGAGCCTTTTCTTAA

Protein sequence

MDIHRIESNEHIGFYRFAAATLVGVCSGEGRRKRAENLHPPFFPISNSPNSMAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLASLRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDTGPGKSFRNFKFDTASILQSMERAFS*
Homology
BLAST of CSPI06G21370 vs. ExPASy Swiss-Prot
Match: Q8GW31 (mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 3.4e-123
Identity = 212/295 (71.86%), Postives = 250/295 (84.75%), Query Frame = 0

Query: 52  MAALHHSSSTTP-VKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDN 111
           M+ LH SSS++  + N LP +EL+DDLCSRFVLNVP+ED QSFERILFL+EYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 112 SVERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETF 171
           +VE +P LKSL+LKEFTSL+F SCDVLRPYV HIDDIFKDFTSYK RVPVTGAIILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 172 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQ 231
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFD+S LLK+ EYIE +F  Q
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQ 180

Query: 232 RVRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLA 291
           RVRLYI+AGV +D +FAP TKKEISEI WHRLD L+PAS+ VI+ G+SGLKLYM+APFL+
Sbjct: 181 RVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLS 240

Query: 292 SLRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDT 346
           SL+SWIL H  PV   P+ P++ L VW A+ +S+G + T  +ESQ  K+E+   T
Sbjct: 241 SLKSWILKHPSPVARRPNKPLKALCVWNAR-TSVGGNGTATVESQNRKSELRTTT 294

BLAST of CSPI06G21370 vs. ExPASy Swiss-Prot
Match: O13828 (mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dcp2 PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 6.1e-56
Identity = 109/226 (48.23%), Postives = 146/226 (64.60%), Query Frame = 0

Query: 72  ELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEFTSLMF 131
           +++DDL +RF+LN+P E+  S ER+ F IE AHWFYED    +N  L SL L+ F++ +F
Sbjct: 10  QVLDDLSARFILNLPAEEQSSVERLCFQIEQAHWFYEDFIRAQNDQLPSLGLRVFSAKLF 69

Query: 132 KSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWKGSS-WSFPRGK 191
             C +L  +    ++ F DF  YK R+PV GAI+LD + ++C+LVKGWK SS W FP+GK
Sbjct: 70  AHCPLLWKWSKVHEEAFDDFLRYKTRIPVRGAIMLDMSMQQCVLVKGWKASSGWGFPKGK 129

Query: 192 KSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGVKDDNLFAPQT 251
             KDE D  CAIREV EETGFD S+ +  +E+I++    Q VRLYII G+  D  F  +T
Sbjct: 130 IDKDESDVDCAIREVYEETGFDCSSRINPNEFIDMTIRGQNVRLYIIPGISLDTRFESRT 189

Query: 252 KKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLASLRSWI 297
           +KEIS+I WH L DL     N      +  K YM+ PFLA L+ WI
Sbjct: 190 RKEISKIEWHNLMDLPTFKKNKPQTMKN--KFYMVIPFLAPLKKWI 233

BLAST of CSPI06G21370 vs. ExPASy Swiss-Prot
Match: Q9CYC6 (m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2)

HSP 1 Score: 201.1 bits (510), Expect = 2.3e-50
Identity = 97/231 (41.99%), Postives = 137/231 (59.31%), Query Frame = 0

Query: 70  PQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEFTSL 129
           P  ++DDLCSRF+L++P E+  +  R+ F IE AHWFY D  ++  P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKA 68

Query: 130 MFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWKG-SSWSFPR 189
           +F  C  L P    ++ I  ++  YK+ VP  GAIILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPQGEDVEKILDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 190 GKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGVKDDNLFAP 249
           GK +K+E  H CA REV EETGFDI + + K +YIE+    Q  RLYII GV  D  F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGVPKDTKFNP 188

Query: 250 QTKKEISEIAWHRLDDLEPASDNVISRGMSGL---KLYMIAPFLASLRSWI 297
           +T++EI  I W  ++ L    +++  +   GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of CSPI06G21370 vs. ExPASy Swiss-Prot
Match: Q8IU60 (m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3)

HSP 1 Score: 200.3 bits (508), Expect = 3.9e-50
Identity = 95/231 (41.13%), Postives = 137/231 (59.31%), Query Frame = 0

Query: 70  PQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEFTSL 129
           P  ++DDLCSRF+L++P E+  +  R+ F IE AHWFY D  ++  P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKA 68

Query: 130 MFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWKG-SSWSFPR 189
           +F  C  L P    ++ +  ++  YK+ VP  GAIILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 190 GKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGVKDDNLFAP 249
           GK +K+E  H CA REV EETGFDI + + K +YIE+    Q  RLYII G+  D  F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNP 188

Query: 250 QTKKEISEIAWHRLDDLEPASDNVISRGMSGL---KLYMIAPFLASLRSWI 297
           +T++EI  I W  ++ L    +++  +   GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of CSPI06G21370 vs. ExPASy Swiss-Prot
Match: Q5REQ8 (m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.9e-50
Identity = 95/231 (41.13%), Postives = 137/231 (59.31%), Query Frame = 0

Query: 70  PQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEFTSL 129
           P  ++DDLCSRF+L++P E+  +  R+ F IE AHWFY D  ++  P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLSQCGIRDFAKA 68

Query: 130 MFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWKG-SSWSFPR 189
           +F  C  L P    ++ +  ++  YK+ VP  GAIILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPEGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 190 GKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGVKDDNLFAP 249
           GK +K+E  H CA REV EETGFDI + + K +YIE+    Q  RLYII G+  D  F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNP 188

Query: 250 QTKKEISEIAWHRLDDLEPASDNVISRGMSGL---KLYMIAPFLASLRSWI 297
           +T++EI  I W  ++ L    +++  +   GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of CSPI06G21370 vs. ExPASy TrEMBL
Match: A0A0A0KHG2 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G405350 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 8.8e-215
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MDIHRIESNEHIGFYRFAAATLVGVCSGEGRRKRAENLHPPFFPISNSPNSMAALHHSSS 60
           MDIHRIESNEHIGFYRFAAATLVGVCSGEGRRKRAENLHPPFFPISNSPNSMAALHHSSS
Sbjct: 1   MDIHRIESNEHIGFYRFAAATLVGVCSGEGRRKRAENLHPPFFPISNSPNSMAALHHSSS 60

Query: 61  TTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKS 120
           TTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKS
Sbjct: 61  TTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKS 120

Query: 121 LNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWK 180
           LNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWK
Sbjct: 121 LNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWK 180

Query: 181 GSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGV 240
           GSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGV
Sbjct: 181 GSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGV 240

Query: 241 KDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLASLRSWILTHH 300
           KDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLASLRSWILTHH
Sbjct: 241 KDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLASLRSWILTHH 300

Query: 301 PPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDTGPGKSFRNFKFDTAS 360
           PPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDTGPGKSFRNFKFDTAS
Sbjct: 301 PPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDTGPGKSFRNFKFDTAS 360

Query: 361 ILQSMERAFS 371
           ILQSMERAFS
Sbjct: 361 ILQSMERAFS 370

BLAST of CSPI06G21370 vs. ExPASy TrEMBL
Match: A0A5D3BM04 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00650 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.7e-176
Identity = 306/318 (96.23%), Postives = 312/318 (98.11%), Query Frame = 0

Query: 52  MAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 111
           MAAL HSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALQHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 112 VERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFE 171
           VERNPSLKSLNLKEFTSL+FKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAI+LDETFE
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIVLDETFE 120

Query: 172 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQR 231
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLL KSEYIEVIFG QR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLNKSEYIEVIFGQQR 180

Query: 232 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLAS 291
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRLDDLEPASDNVISRGM+GLKLYM+APFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240

Query: 292 LRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDTGPGKSF 351
           LRSWILTHHPPV  NPDIPIRGLTVWKAKNSSI SSSTIIMESQISKAEV+VDTGPGKSF
Sbjct: 241 LRSWILTHHPPVHSNPDIPIRGLTVWKAKNSSISSSSTIIMESQISKAEVDVDTGPGKSF 300

Query: 352 RNFKFDTASILQSMERAF 370
           RNFKFDTASILQSMERAF
Sbjct: 301 RNFKFDTASILQSMERAF 318

BLAST of CSPI06G21370 vs. ExPASy TrEMBL
Match: A0A1S3C3F4 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103496421 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.7e-176
Identity = 306/318 (96.23%), Postives = 312/318 (98.11%), Query Frame = 0

Query: 52  MAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 111
           MAAL HSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALQHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 112 VERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFE 171
           VERNPSLKSLNLKEFTSL+FKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAI+LDETFE
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIVLDETFE 120

Query: 172 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQR 231
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLL KSEYIEVIFG QR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLNKSEYIEVIFGQQR 180

Query: 232 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLAS 291
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRLDDLEPASDNVISRGM+GLKLYM+APFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240

Query: 292 LRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDTGPGKSF 351
           LRSWILTHHPPV  NPDIPIRGLTVWKAKNSSI SSSTIIMESQISKAEV+VDTGPGKSF
Sbjct: 241 LRSWILTHHPPVHSNPDIPIRGLTVWKAKNSSISSSSTIIMESQISKAEVDVDTGPGKSF 300

Query: 352 RNFKFDTASILQSMERAF 370
           RNFKFDTASILQSMERAF
Sbjct: 301 RNFKFDTASILQSMERAF 318

BLAST of CSPI06G21370 vs. ExPASy TrEMBL
Match: A0A6J1JIL9 (mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487274 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.5e-166
Identity = 290/324 (89.51%), Postives = 306/324 (94.44%), Query Frame = 0

Query: 52  MAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 111
           MAALH SSST PVKNGLPPQEL+DDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALHSSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 112 VERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFE 171
           VERNPSLKSLNLKEFTSL+FKSCDVL+PYVAHIDDIFKDFTSYKVRVPVTGAIILDET E
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSE 120

Query: 172 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQR 231
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDI+NL+ KSEYIEVIFGPQR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQR 180

Query: 232 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLAS 291
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRL DLEPA DNVISRGM+GLKLYM+APFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLAS 240

Query: 292 LRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEV-----DTG 351
           LRSWI TH PPV   PD+ IRGLTVWKAKNSSIGSSSTI++ESQ+SK +++V     DTG
Sbjct: 241 LRSWISTHQPPVASKPDVSIRGLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTG 300

Query: 352 PGKSFRNFKFDTASILQSMERAFS 371
           PGKSFRNFKFDTASI+QSMERAFS
Sbjct: 301 PGKSFRNFKFDTASIIQSMERAFS 324

BLAST of CSPI06G21370 vs. ExPASy TrEMBL
Match: A0A6J1HJG1 (mRNA-decapping enzyme subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464573 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.8e-166
Identity = 289/324 (89.20%), Postives = 305/324 (94.14%), Query Frame = 0

Query: 52  MAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 111
           MAALH SS T PVKNGLPPQEL+DDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALHSSSCTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 112 VERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFE 171
           VERNPSLKSLNLKEFTSL+FKSCDVL+PYVAHIDDIFKDFTSYKVRVPVTGAIILDET E
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSE 120

Query: 172 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQR 231
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDI+NL+ KSEYIEVIFGPQR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQR 180

Query: 232 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLAS 291
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRL DLEPA DNVISRGM+GLKLYM+APFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLAS 240

Query: 292 LRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEV-----DTG 351
           LRSWI TH PPV   PD+ IRGLTVWKAKNSSIGSSSTI++ESQ+SK +++V     DTG
Sbjct: 241 LRSWISTHQPPVASKPDVSIRGLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTG 300

Query: 352 PGKSFRNFKFDTASILQSMERAFS 371
           PGKSFRNFKFDTASI+QSMERAFS
Sbjct: 301 PGKSFRNFKFDTASIIQSMERAFS 324

BLAST of CSPI06G21370 vs. NCBI nr
Match: XP_004149909.2 (mRNA-decapping enzyme subunit 2 [Cucumis sativus] >KGN47822.1 hypothetical protein Csa_003258 [Cucumis sativus])

HSP 1 Score: 755.7 bits (1950), Expect = 1.8e-214
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MDIHRIESNEHIGFYRFAAATLVGVCSGEGRRKRAENLHPPFFPISNSPNSMAALHHSSS 60
           MDIHRIESNEHIGFYRFAAATLVGVCSGEGRRKRAENLHPPFFPISNSPNSMAALHHSSS
Sbjct: 1   MDIHRIESNEHIGFYRFAAATLVGVCSGEGRRKRAENLHPPFFPISNSPNSMAALHHSSS 60

Query: 61  TTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKS 120
           TTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKS
Sbjct: 61  TTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKS 120

Query: 121 LNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWK 180
           LNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWK
Sbjct: 121 LNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFERCLLVKGWK 180

Query: 181 GSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGV 240
           GSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGV
Sbjct: 181 GSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQRVRLYIIAGV 240

Query: 241 KDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLASLRSWILTHH 300
           KDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLASLRSWILTHH
Sbjct: 241 KDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLASLRSWILTHH 300

Query: 301 PPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDTGPGKSFRNFKFDTAS 360
           PPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDTGPGKSFRNFKFDTAS
Sbjct: 301 PPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDTGPGKSFRNFKFDTAS 360

Query: 361 ILQSMERAFS 371
           ILQSMERAFS
Sbjct: 361 ILQSMERAFS 370

BLAST of CSPI06G21370 vs. NCBI nr
Match: XP_008456478.1 (PREDICTED: mRNA-decapping enzyme subunit 2-like [Cucumis melo] >KAA0036467.1 mRNA-decapping enzyme subunit 2-like [Cucumis melo var. makuwa] >TYK00020.1 mRNA-decapping enzyme subunit 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 627.1 bits (1616), Expect = 9.7e-176
Identity = 306/318 (96.23%), Postives = 312/318 (98.11%), Query Frame = 0

Query: 52  MAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 111
           MAAL HSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALQHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 112 VERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFE 171
           VERNPSLKSLNLKEFTSL+FKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAI+LDETFE
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIVLDETFE 120

Query: 172 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQR 231
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLL KSEYIEVIFG QR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLNKSEYIEVIFGQQR 180

Query: 232 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLAS 291
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRLDDLEPASDNVISRGM+GLKLYM+APFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240

Query: 292 LRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDTGPGKSF 351
           LRSWILTHHPPV  NPDIPIRGLTVWKAKNSSI SSSTIIMESQISKAEV+VDTGPGKSF
Sbjct: 241 LRSWILTHHPPVHSNPDIPIRGLTVWKAKNSSISSSSTIIMESQISKAEVDVDTGPGKSF 300

Query: 352 RNFKFDTASILQSMERAF 370
           RNFKFDTASILQSMERAF
Sbjct: 301 RNFKFDTASILQSMERAF 318

BLAST of CSPI06G21370 vs. NCBI nr
Match: XP_038883995.1 (mRNA-decapping enzyme subunit 2-like [Benincasa hispida])

HSP 1 Score: 605.5 bits (1560), Expect = 3.0e-169
Identity = 296/322 (91.93%), Postives = 309/322 (95.96%), Query Frame = 0

Query: 52  MAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 111
           MAALHHSSST PVKN LP QEL+DDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALHHSSSTAPVKNVLPSQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 112 VERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFE 171
           VERNPSLKSLNLKEFTSL+FKSCDVL+PYVAHIDDIFKDFTSYKVRVPVTGAII+DETFE
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120

Query: 172 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQR 231
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDI+NLL KSEYIEVIFG QR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180

Query: 232 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLAS 291
           VRLYIIAGVK DN+FAPQTKKEISEIAWHRLDDLEPASDNVISRGM+GLKLYM+APFLAS
Sbjct: 181 VRLYIIAGVKVDNIFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240

Query: 292 LRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEV---DTGPG 351
           LRSWI THHPPV PNPD+ IRGLTVWKAKNSSIGSSSTIIMESQ+SK EV+V   DTGPG
Sbjct: 241 LRSWISTHHPPVHPNPDVSIRGLTVWKAKNSSIGSSSTIIMESQLSKQEVDVSIPDTGPG 300

Query: 352 KSFRNFKFDTASILQSMERAFS 371
           +SFRNFKFDTASILQSM+RAFS
Sbjct: 301 RSFRNFKFDTASILQSMDRAFS 322

BLAST of CSPI06G21370 vs. NCBI nr
Match: XP_022990407.1 (mRNA-decapping enzyme subunit 2-like [Cucurbita maxima])

HSP 1 Score: 595.5 bits (1534), Expect = 3.1e-166
Identity = 290/324 (89.51%), Postives = 306/324 (94.44%), Query Frame = 0

Query: 52  MAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 111
           MAALH SSST PVKNGLPPQEL+DDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALHSSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 112 VERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFE 171
           VERNPSLKSLNLKEFTSL+FKSCDVL+PYVAHIDDIFKDFTSYKVRVPVTGAIILDET E
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSE 120

Query: 172 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQR 231
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDI+NL+ KSEYIEVIFGPQR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQR 180

Query: 232 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLAS 291
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRL DLEPA DNVISRGM+GLKLYM+APFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLAS 240

Query: 292 LRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEV-----DTG 351
           LRSWI TH PPV   PD+ IRGLTVWKAKNSSIGSSSTI++ESQ+SK +++V     DTG
Sbjct: 241 LRSWISTHQPPVASKPDVSIRGLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTG 300

Query: 352 PGKSFRNFKFDTASILQSMERAFS 371
           PGKSFRNFKFDTASI+QSMERAFS
Sbjct: 301 PGKSFRNFKFDTASIIQSMERAFS 324

BLAST of CSPI06G21370 vs. NCBI nr
Match: XP_022964616.1 (mRNA-decapping enzyme subunit 2-like [Cucurbita moschata] >XP_023540752.1 mRNA-decapping enzyme subunit 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 593.6 bits (1529), Expect = 1.2e-165
Identity = 289/324 (89.20%), Postives = 305/324 (94.14%), Query Frame = 0

Query: 52  MAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 111
           MAALH SS T PVKNGLPPQEL+DDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALHSSSCTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 112 VERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFE 171
           VERNPSLKSLNLKEFTSL+FKSCDVL+PYVAHIDDIFKDFTSYKVRVPVTGAIILDET E
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSE 120

Query: 172 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQR 231
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDI+NL+ KSEYIEVIFGPQR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQR 180

Query: 232 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLAS 291
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRL DLEPA DNVISRGM+GLKLYM+APFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLAS 240

Query: 292 LRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEV-----DTG 351
           LRSWI TH PPV   PD+ IRGLTVWKAKNSSIGSSSTI++ESQ+SK +++V     DTG
Sbjct: 241 LRSWISTHQPPVASKPDVSIRGLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTG 300

Query: 352 PGKSFRNFKFDTASILQSMERAFS 371
           PGKSFRNFKFDTASI+QSMERAFS
Sbjct: 301 PGKSFRNFKFDTASIIQSMERAFS 324

BLAST of CSPI06G21370 vs. TAIR 10
Match: AT5G13570.1 (decapping 2 )

HSP 1 Score: 443.0 bits (1138), Expect = 2.4e-124
Identity = 212/295 (71.86%), Postives = 250/295 (84.75%), Query Frame = 0

Query: 52  MAALHHSSSTTP-VKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDN 111
           M+ LH SSS++  + N LP +EL+DDLCSRFVLNVP+ED QSFERILFL+EYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 112 SVERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETF 171
           +VE +P LKSL+LKEFTSL+F SCDVLRPYV HIDDIFKDFTSYK RVPVTGAIILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 172 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQ 231
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFD+S LLK+ EYIE +F  Q
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQ 180

Query: 232 RVRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLA 291
           RVRLYI+AGV +D +FAP TKKEISEI WHRLD L+PAS+ VI+ G+SGLKLYM+APFL+
Sbjct: 181 RVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLS 240

Query: 292 SLRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQISKAEVEVDT 346
           SL+SWIL H  PV   P+ P++ L VW A+ +S+G + T  +ESQ  K+E+   T
Sbjct: 241 SLKSWILKHPSPVARRPNKPLKALCVWNAR-TSVGGNGTATVESQNRKSELRTTT 294

BLAST of CSPI06G21370 vs. TAIR 10
Match: AT5G13570.2 (decapping 2 )

HSP 1 Score: 433.7 bits (1114), Expect = 1.5e-121
Identity = 212/308 (68.83%), Postives = 250/308 (81.17%), Query Frame = 0

Query: 52  MAALHHSSSTTP-VKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDN 111
           M+ LH SSS++  + N LP +EL+DDLCSRFVLNVP+ED QSFERILFL+EYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 112 SVERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETF 171
           +VE +P LKSL+LKEFTSL+F SCDVLRPYV HIDDIFKDFTSYK RVPVTGAIILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 172 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRE-------------VLEETGFDISNLL 231
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRE             VLEETGFD+S LL
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRELSSAILLVNVAFQVLEETGFDVSKLL 180

Query: 232 KKSEYIEVIFGPQRVRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGM 291
           K+ EYIE +F  QRVRLYI+AGV +D +FAP TKKEISEI WHRLD L+PAS+ VI+ G+
Sbjct: 181 KREEYIEFVFRQQRVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGV 240

Query: 292 SGLKLYMIAPFLASLRSWILTHHPPVRPNPDIPIRGLTVWKAKNSSIGSSSTIIMESQIS 346
           SGLKLYM+APFL+SL+SWIL H  PV   P+ P++ L VW A+ +S+G + T  +ESQ  
Sbjct: 241 SGLKLYMVAPFLSSLKSWILKHPSPVARRPNKPLKALCVWNAR-TSVGGNGTATVESQNR 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GW313.4e-12371.86mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=... [more]
O138286.1e-5648.23mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q9CYC62.3e-5041.99m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2[more]
Q8IU603.9e-5041.13m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3[more]
Q5REQ83.9e-5041.13m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KHG28.8e-215100.00Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G40... [more]
A0A5D3BM044.7e-17696.23mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3C3F44.7e-17696.23mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103496421 PE=... [more]
A0A6J1JIL91.5e-16689.51mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487274... [more]
A0A6J1HJG15.8e-16689.20mRNA-decapping enzyme subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114645... [more]
Match NameE-valueIdentityDescription
XP_004149909.21.8e-214100.00mRNA-decapping enzyme subunit 2 [Cucumis sativus] >KGN47822.1 hypothetical prote... [more]
XP_008456478.19.7e-17696.23PREDICTED: mRNA-decapping enzyme subunit 2-like [Cucumis melo] >KAA0036467.1 mRN... [more]
XP_038883995.13.0e-16991.93mRNA-decapping enzyme subunit 2-like [Benincasa hispida][more]
XP_022990407.13.1e-16689.51mRNA-decapping enzyme subunit 2-like [Cucurbita maxima][more]
XP_022964616.11.2e-16589.20mRNA-decapping enzyme subunit 2-like [Cucurbita moschata] >XP_023540752.1 mRNA-d... [more]
Match NameE-valueIdentityDescription
AT5G13570.12.4e-12471.86decapping 2 [more]
AT5G13570.21.5e-12168.83decapping 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007722mRNA decapping protein 2, Box A domainSMARTSM01125DCP2_2coord: 71..155
e-value: 6.3E-43
score: 158.6
IPR007722mRNA decapping protein 2, Box A domainPFAMPF05026DCP2coord: 73..154
e-value: 2.0E-28
score: 98.5
IPR036189mRNA decapping protein 2, Box A domain superfamilyGENE3D1.10.10.1050Dcp2, box A domaincoord: 73..156
e-value: 7.3E-87
score: 292.3
IPR036189mRNA decapping protein 2, Box A domain superfamilySUPERFAMILY140586Dcp2 domain-likecoord: 66..155
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 157..297
e-value: 7.3E-87
score: 292.3
NoneNo IPR availablePANTHERPTHR23114M7GPPPN-MRNA HYDROLASEcoord: 60..316
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 158..270
e-value: 1.7E-14
score: 54.0
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 156..282
score: 12.96505
IPR020084NUDIX hydrolase, conserved sitePROSITEPS00893NUDIX_BOXcoord: 189..210
IPR044099mRNA decapping enzyme 2 , NUDIX hydrolase domainCDDcd03672Dcp2pcoord: 158..299
e-value: 4.69183E-72
score: 219.417
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 158..299

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G21370.1CSPI06G21370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA
biological_process GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050072 m7G(5')pppN diphosphatase activity
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0016787 hydrolase activity