CSPI06G21300 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G21300
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionLINE-1 retrotransposable element ORF2 protein
LocationChr6: 19288370 .. 19292500 (+)
RNA-Seq ExpressionCSPI06G21300
SyntenyCSPI06G21300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTTGTTTAGGTTGTTGATTCTGCACCTACTGGGTCGGTTGAAAATGCATTTGTGTTTCAACAATGAAGTAAATACACAACAACCTACACACAATGTATCACAGATTATTGATGTTGATTCCTTTGATTCACCAAATGATGCAGTACCTATTGATGTCGAAGTAAAGTTCAGAACTAAGTCAATACTGAAGAAAGCGATTTATATGTTGGCTGTGAACAATAGTTTTGAATTTGTTACAGTTAGGTCCAACCGCACATCATTTGACATTCGATGCAAAGATATGTTGTGTTCAAGGTATTTACGTGCTTCTGTGTTAAAAAAAGTGATATTTGGATTATTCGTAAGTTTATGAATACACACCAGTGTGCCGTTGACATTGTTAAAAATGATCATAGGCAAGCAACATCCTGGAATATTGTTTTTGAGTGTACGAAGTCATTTTTTAAAATGAATGACAAGGCTCCATGCCGCCCTTCTGATGTTATTAATTACATGAAAATTAATCACGGGGTAAATTTAAGTTATGATAAGGCCTGGAGAGAACGTGAGATTGCATTGAATTCCATTAGGGGTGCCCCGGAGGAATTGTATGCAGTGTTGGGGGCATTCACAGATGCATTGATTAGGAATAATCCATGTGTAATTTATACATCAAATATTGTGTATTTGCTATTAGATATTTTACTCATTTTGGAAATACAGTGTAATTTTAATATCACATTTATTGCATAATTAATAGGAATGTATACGGCTGAAGAATCAGACGATGAATGTCGATTCAAGTTCTATTTCATGGCCCTTGCAGCTTTAATTGATGCATGAAATTATTGTGTGCTACTTATTTCAGTTGATGGTGCAGCGTTGAAGACAAAATATCTTGATACGCTCATTTCTGTTTGTACTATTAATGGAAATTCTCAAATTGTGCCACTAGCTATTCAGAGAATAACTTGTCATGGCACTACTACAAAAAGAGGATTACTTGACGCCTGCAATTTAGAATTACTTGACAGTTTTAATAAAAACTGTCAAGTAAAATGAAAAAATGTCAAGAATAACAAAAAACTAGAAAAATACATCATTTTTCAATTTTTTTCTCTAATACTTGACAGGTTAGAAGCATCGAGAACAATTTTACTTACTTGACAATTTTTAAATGCAAAAAATTACCTAATTTTAAATAATTTTTTCCCTTGTTTAAAAAAAATATATTCCCAAAATTTTCTTCTTCTTTATTTTCTCCATATTCCCAAATTTCCTTTTTCCCCCAAATTTCTTAACAAATTCATTTTTCCACTATTCCTCCTCTTCTTCACATGCATTATACTTAACCCTTTTGCCGTTGCACTATTTTTCTTCTACACAAAAAATTTCCACCAATCCAAGCAACAATGTCCAACAGAGGGAAAGGAGGAAAAGGTCTTGAAAAGGGAAAGAAACTCGAATGTTCTCAAGATCTTCCTAAAAAATTTCATTCGCAACATCGTCACTTACACTGAGCACACTCTCCAGAAGACTGACACCACCATGGATGTTGCGTACGCGCTCAATAGGCAAGGACGTACTCTATAAAACTTCGGAAGTTTGGGTTAATGAAACCTATGGCTTGGATTTAGGGGTTTTGTGTTACAATTATTTTTTTTTAATGATATTTGTAATCTACAATCGTTTTTCTTGTTTCCATGTCTTTGTTATAAACAGGTCAACTTCCAAAAATGTAATTGATGAAGAAACTGGAAAACTTTGGTTGTGGGATTAATGAAGAAATTGAAAACTTGCAGAAATGGTGAAATGATTCTCCATGCCTACTTCTACGTTGTGGTAAATACCTAAAAATTTGGTTTTCTGTGCTTTTAGTCTAATGAATTTCTTAAGCAATTTGTATTTGTTCTTTTCTTTCTCTTTTTTCCCTTTTTCAATTTCTTGTTTGAAGTGCAAAACAGTATTCCCTAAAATCTTCTCTTCTTTGGATTGAGAACTTAGAGTTTTTAGATGAACATTGTGAGGTTTGAAAATTTGGGAGTTTTGCTTAAGGGGATGTTTTAGCTGATTGAATTTTGTATTTAACTCTTTGGTGAATTCAGAGTAGTTGGATTAGGATTTTCTCACTCACTCCCAATTCTAATTCCATTTCCAAAATATCATTCTCTTTGCAATCTCCGCCGTCTGCAACCATCCCCCATCTCTCTCGTCGTCGCCCTCCTTCTCTTCCTTCCTTCATTCAGGTCAATTTCCCTTATCTTTTCTTTTTTTTACATTTGTCATAATCCTTCGTTATGATATAGGTGAGCTGACTAATTCAATAAAAAGAAATTGGTTTTCTCAGCTGGATTATTCACACCCTCCATTCTAATTTCCCATTCCCCTACATCTCTTCCTTTAACTTCTGCTTCCTCCGGCTTCTCCTCTTCCCTCGACGCTGGTTTTTTCTCTCTTTACCCTTTTCCGTTATTTTTAGCTCAACAACGGACTGCTTTTTTAGGTATTTTTAGCTCAACAAGAAGCGGCGGGGAGTTCTTCGTAAGGCAGAGGCTACGGCGGAGAAGATGGGAGTGCCAAGATCAGGAGGGGAGCGTTACTGGCAATGAAATATGTGTTTAGAAGAAAGTAAAAACCTGCTGAGACGAGTGCTTATACTAACAAGACGAATTCCAAGGGAGTAACCTTTGCGTTGTGAATTAAGAAGGAAGCCACCTGCCGTATTTCAAGGAAGAAAATGATTATTTGCAAAAGTTTAAATTCTGAAGGCATGAGGTGGAGTTTCACAAGAATGAGATGTTTTTTTATTCTAGGTATTTGGAGAGATTTTTTTTTTTGTAGCATTTGTTTTTCAATTAGCGTACACAGGAGTTTATGTTAGTGGGGTAAACAATTTCCATTTTTTAGTTTGAATAGGGGCATTTGAAGGATTTATTTATTTTATATTTTTTATATTTTATTATTCTAACAATTTAAAATTGTTTTTGCTATTTTTCCAAAGTAAAACATATAATTTGTCATTCTTCTTGACAGCCAAGGTAAAACAAAATAATGAAGGACAGTTTTGGGTCGAAGCTCAAAGGTTTGAGGACTTTTTGGTTTGAAAAATTTAGATAAAAAACCTAAATTTAATACGAAATTTCACAAATTCTCTAAACTAAAATTTATTTTTTTAATTAGGATTTCGTATATTTTTACGATTTTATTATGGAAGTAGTCCATTGAAAAAAATTTCATAGATCACTTCAAGAAGATTTATACTATGAATAGACCTATTAAATGGGTGATTGAAAATCTCATTTGAGCACCCATTACAGATAGTCTCAAGGATCAACTTTGCCTCCCTTTTCGAGAGTCAGAAATTCATTCAACTCTTAGCTCTTTTGCAAACAACGAAACCCCGGGTCCAGATAGGTTTACTATTGAATTCCTAAAAAAACATTGGAAAATTCTGAAACAAGACATCAAGATTGTTTTTGTTGATTTCTTTCAAAAGGGGATCATAAATAATATTGTAAATGAGACTTACATTGCCCTTATTGCTAAGAAAGAAAAATGCTCCAAAGCTGATGACTACAGACCTATTAGCCTTAGAACAGCCTTATACAAACTAATTGCAAAAACCATGACAGAAAGACTCAAAGTCACCCTCCCTCACACCATATCAGACCATCAGATGGCCTTTGTTAAAGAGAGGCAGATTACTGATGCCATTCTCATTGTCAATGAAGCCATTGATTACTGGAAAGTCAAGAAAACAAGGGGATTTGTGATCAAGCTGGATATCGCAAAGGCTTTCGACAAAATCAATTGGAGCTTTATAGATTATATGTTAATGAAAAAAAACTACTCTGGACAGTGGAGGAGGTGGATTCATTCGTGTATTAGCAGTGTACATTACTCAATCCTCATCAATGGAAAACCCAGCGGTAAAATCAAACCTACTAGAGGCATACGACGAGGTGATCCACTTTCTCCTTTCATATTCGTACTTGCCATGGATTATTTCAGCAGGTTGATTCGACATGTTCAACAACAAGGTAAAATTAAAGGAGTCTGCTTCAATAATGAATTCAACCTCACACATCTGCTTCAATCTATCAAAGTCCACCATATCTCCCATAAATGTCGACAACTGCAGAACTGA

mRNA sequence

ATGATTTTGTTTAGGTTGTTGATTCTGCACCTACTGGGTCGGTTGAAAATGCATTTGTGTTTCAACAATGAAGTAAATACACAACAACCTACACACAATGTATCACAGATTATTGATGTTGATTCCTTTGATTCACCAAATGATGCAGTACCTATTGATGTCGAAGTAAAGTTCAGAACTAAGTCAATACTGAAGAAAGCGATTTATATGTTGGCTGTGAACAATAGTTTTGAATTTGTTACAGTTAGGTCCAACCGCACATCATTTGACATTCGATGCAAAGATATGTTGTGTTCAAGGCAAGCAACATCCTGGAATATTGTTTTTGAGTGTACGAAGTCATTTTTTAAAATGAATGACAAGGCTCCATGCCGCCCTTCTGATGTTATTAATTACATGAAAATTAATCACGGGGTAAATTTAAGTTATGATAAGGCCTGGAGAGAACGTGAGATTGCATTGAATTCCATTAGGGGTGCCCCGGAGGAATTGTATGCAGTGTTGGGGGCATTCACAGATGCATTGATTAGGAATAATCCATGTGAATGTATACGGCTGAAGAATCAGACGATGAATGTCGATTCAATTGATGGTGCAGCGTTGAAGACAAAATATCTTGATACGCTCATTTCTGTTTGTACTATTAATGGAAATTCTCAAATTGTGCCACTAGCTATTCAGAGAATAACTTGTCATGGCACTACTACAAAAAGAGGATTACTTGACGCCTGCAATTTAGAATTACTTGACAGTTTTAATAAAAACTGTCAACAACAATGTCCAACAGAGGGAAAGGAGGAAAAGGTCTTGAAAAGGGAAAGAAACTCGAATGTTCTCAAGATCTTCCTAAAAAATTTCATTCGCAACATCGTCACTTACACTGAGCACACTCTCCAGAAGACTGACACCACCATGGATGTTGCTGCAAAACAGTATTCCCTAAAATCTTCTCTTCTTTGGATTGAGAACTTAGAGTTTTTAGATGAACATTGTGAGAGTAGTTGGATTAGGATTTTCTCACTCACTCCCAATTCTAATTCCATTTCCAAAATATCATTCTCTTTGCAATCTCCGCCGTCTGCAACCATCCCCCATCTCTCTCGTCGTCGCCCTCCTTCTCTTCCTTCCTTCATTCAGCTCAACAAGAAGCGGCGGGGAGTTCTTCGTAAGGCAGAGGCTACGGCGGAGAAGATGGGAGTGCCAAGATCAGGAGGGGAGCCACCCATTACAGATAGTCTCAAGGATCAACTTTGCCTCCCTTTTCGAGAGTCAGAAATTCATTCAACTCTTAGCTCTTTTGCAAACAACGAAACCCCGGGTCCAGATAGGTTTACTATTGAATTCCTAAAAAAACATTGGAAAATTCTGAAACAAGACATCAAGATTGTTTTTGTTGATTTCTTTCAAAAGGGGATCATAAATAATATTGTAAATGAGACTTACATTGCCCTTATTGCTAAGAAAGAAAAATGCTCCAAAGCTGATGACTACAGACCTATTAGCCTTAGAACAGCCTTATACAAACTAATTGCAAAAACCATGACAGAAAGACTCAAAGTCACCCTCCCTCACACCATATCAGACCATCAGATGGCCTTTGTTAAAGAGAGGCAGATTACTGATGCCATTCTCATTGTCAATGAAGCCATTGATTACTGGAAAGTCAAGAAAACAAGGGGATTTGTGATCAAGCTGGATATCGCAAAGGCTTTCGACAAAATCAATTGGAGCTTTATAGATTATATGTTAATGAAAAAAAACTACTCTGGACAGTGGAGGAGGTGGATTCATTCGTGTATTAGCAGTGTACATTACTCAATCCTCATCAATGGAAAACCCAGCGGTAAAATCAAACCTACTAGAGGCATACGACGAGGTGATCCACTTTCTCCTTTCATATTCGTACTTGCCATGGATTATTTCAGCAGGTTGATTCGACATGTTCAACAACAAGGTAAAATTAAAGGAGTCTGCTTCAATAATGAATTCAACCTCACACATCTGCTTCAATCTATCAAAGTCCACCATATCTCCCATAAATGTCGACAACTGCAGAACTGA

Coding sequence (CDS)

ATGATTTTGTTTAGGTTGTTGATTCTGCACCTACTGGGTCGGTTGAAAATGCATTTGTGTTTCAACAATGAAGTAAATACACAACAACCTACACACAATGTATCACAGATTATTGATGTTGATTCCTTTGATTCACCAAATGATGCAGTACCTATTGATGTCGAAGTAAAGTTCAGAACTAAGTCAATACTGAAGAAAGCGATTTATATGTTGGCTGTGAACAATAGTTTTGAATTTGTTACAGTTAGGTCCAACCGCACATCATTTGACATTCGATGCAAAGATATGTTGTGTTCAAGGCAAGCAACATCCTGGAATATTGTTTTTGAGTGTACGAAGTCATTTTTTAAAATGAATGACAAGGCTCCATGCCGCCCTTCTGATGTTATTAATTACATGAAAATTAATCACGGGGTAAATTTAAGTTATGATAAGGCCTGGAGAGAACGTGAGATTGCATTGAATTCCATTAGGGGTGCCCCGGAGGAATTGTATGCAGTGTTGGGGGCATTCACAGATGCATTGATTAGGAATAATCCATGTGAATGTATACGGCTGAAGAATCAGACGATGAATGTCGATTCAATTGATGGTGCAGCGTTGAAGACAAAATATCTTGATACGCTCATTTCTGTTTGTACTATTAATGGAAATTCTCAAATTGTGCCACTAGCTATTCAGAGAATAACTTGTCATGGCACTACTACAAAAAGAGGATTACTTGACGCCTGCAATTTAGAATTACTTGACAGTTTTAATAAAAACTGTCAACAACAATGTCCAACAGAGGGAAAGGAGGAAAAGGTCTTGAAAAGGGAAAGAAACTCGAATGTTCTCAAGATCTTCCTAAAAAATTTCATTCGCAACATCGTCACTTACACTGAGCACACTCTCCAGAAGACTGACACCACCATGGATGTTGCTGCAAAACAGTATTCCCTAAAATCTTCTCTTCTTTGGATTGAGAACTTAGAGTTTTTAGATGAACATTGTGAGAGTAGTTGGATTAGGATTTTCTCACTCACTCCCAATTCTAATTCCATTTCCAAAATATCATTCTCTTTGCAATCTCCGCCGTCTGCAACCATCCCCCATCTCTCTCGTCGTCGCCCTCCTTCTCTTCCTTCCTTCATTCAGCTCAACAAGAAGCGGCGGGGAGTTCTTCGTAAGGCAGAGGCTACGGCGGAGAAGATGGGAGTGCCAAGATCAGGAGGGGAGCCACCCATTACAGATAGTCTCAAGGATCAACTTTGCCTCCCTTTTCGAGAGTCAGAAATTCATTCAACTCTTAGCTCTTTTGCAAACAACGAAACCCCGGGTCCAGATAGGTTTACTATTGAATTCCTAAAAAAACATTGGAAAATTCTGAAACAAGACATCAAGATTGTTTTTGTTGATTTCTTTCAAAAGGGGATCATAAATAATATTGTAAATGAGACTTACATTGCCCTTATTGCTAAGAAAGAAAAATGCTCCAAAGCTGATGACTACAGACCTATTAGCCTTAGAACAGCCTTATACAAACTAATTGCAAAAACCATGACAGAAAGACTCAAAGTCACCCTCCCTCACACCATATCAGACCATCAGATGGCCTTTGTTAAAGAGAGGCAGATTACTGATGCCATTCTCATTGTCAATGAAGCCATTGATTACTGGAAAGTCAAGAAAACAAGGGGATTTGTGATCAAGCTGGATATCGCAAAGGCTTTCGACAAAATCAATTGGAGCTTTATAGATTATATGTTAATGAAAAAAAACTACTCTGGACAGTGGAGGAGGTGGATTCATTCGTGTATTAGCAGTGTACATTACTCAATCCTCATCAATGGAAAACCCAGCGGTAAAATCAAACCTACTAGAGGCATACGACGAGGTGATCCACTTTCTCCTTTCATATTCGTACTTGCCATGGATTATTTCAGCAGGTTGATTCGACATGTTCAACAACAAGGTAAAATTAAAGGAGTCTGCTTCAATAATGAATTCAACCTCACACATCTGCTTCAATCTATCAAAGTCCACCATATCTCCCATAAATGTCGACAACTGCAGAACTGA

Protein sequence

MILFRLLILHLLGRLKMHLCFNNEVNTQQPTHNVSQIIDVDSFDSPNDAVPIDVEVKFRTKSILKKAIYMLAVNNSFEFVTVRSNRTSFDIRCKDMLCSRQATSWNIVFECTKSFFKMNDKAPCRPSDVINYMKINHGVNLSYDKAWREREIALNSIRGAPEELYAVLGAFTDALIRNNPCECIRLKNQTMNVDSIDGAALKTKYLDTLISVCTINGNSQIVPLAIQRITCHGTTTKRGLLDACNLELLDSFNKNCQQQCPTEGKEEKVLKRERNSNVLKIFLKNFIRNIVTYTEHTLQKTDTTMDVAAKQYSLKSSLLWIENLEFLDEHCESSWIRIFSLTPNSNSISKISFSLQSPPSATIPHLSRRRPPSLPSFIQLNKKRRGVLRKAEATAEKMGVPRSGGEPPITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDFFQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTISDHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKNYSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRHVQQQGKIKGVCFNNEFNLTHLLQSIKVHHISHKCRQLQN*
Homology
BLAST of CSPI06G21300 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 9.7e-23
Identity = 86/289 (29.76%), Postives = 135/289 (46.71%), Query Frame = 0

Query: 407 PPITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVD 466
           P +     D L  P    EI + ++S    ++PGPD F+ EF    ++  K+D+  +   
Sbjct: 446 PKLNQDQVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEF----YQTFKEDLIPILHK 505

Query: 467 FFQK----GIINNIVNETYIALIAKKEK-CSKADDYRPISLRTALYKLIAKTMTERLKVT 526
            F K    G + N   E  I LI K +K  +K +++RPISL     K++ K +  R++  
Sbjct: 506 LFHKIEVEGTLPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEH 565

Query: 527 LPHTISDHQMAFVKERQITDAILIVNEAIDYW-KVKKTRGFVIKLDIAKAFDKINWSFID 586
           +   I   Q+ F+   Q    I      I Y  K+K     +I LD  KAFDKI   F+ 
Sbjct: 566 IKAIIHPDQVGFIPGMQGWFNIRKSINVIHYINKLKDKNHMIISLDAEKAFDKIQHPFMI 625

Query: 587 YMLMKKNYSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDY 646
            +L +    G +   I +  S    +I +NG+    I    G R+G PLSP++F + ++ 
Sbjct: 626 KVLERSGIQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEV 685

Query: 647 FSRLIRHVQQQGKIKGVCFNNEFNLTHLLQSIKVHHIS---HKCRQLQN 687
            +R IR   QQ +IKG+    E     LL    + +IS   +  R+L N
Sbjct: 686 LARAIR---QQKEIKGIQIGKEEVKISLLADDMIVYISDPKNSTRELLN 727

BLAST of CSPI06G21300 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 3.7e-22
Identity = 66/242 (27.27%), Postives = 120/242 (49.59%), Query Frame = 0

Query: 405 GEPPITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVF 464
           G P +++  K++L  P    E+   L    +N++PG D  TIEF +  W  L  D   V 
Sbjct: 433 GLPVVSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVL 492

Query: 465 VDFFQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPH 524
            + F+KG +        ++L+ KK       ++RP+SL +  YK++AK ++ RLK  L  
Sbjct: 493 TEAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAE 552

Query: 525 TISDHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLM 584
            I   Q   V  R I D + ++ + + + +        + LD  KAFD+++  ++   L 
Sbjct: 553 VIHPDQSYTVPGRTIFDNVFLIRDLLHFARRTGLSLAFLSLDQEKAFDRVDHQYLIGTLQ 612

Query: 585 KKNYSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRL 644
             ++  Q+  ++ +  +S    + IN   +  +   RG+R+G PLS  ++ LA++ F  L
Sbjct: 613 AYSFGPQFVGYLKTMYASAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIEPFLCL 672

Query: 645 IR 647
           +R
Sbjct: 673 LR 674

BLAST of CSPI06G21300 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.1e-21
Identity = 76/265 (28.68%), Postives = 128/265 (48.30%), Query Frame = 0

Query: 407 PPITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVD 466
           P ++    + L  P   SEI ST+ +    ++PGPD FT EF    ++  K+++  + ++
Sbjct: 438 PRLSQKEVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEF----YQTFKEELVPILLN 497

Query: 467 FFQ----KGIINNIVNETYIALIAKKEK-CSKADDYRPISLRTALYKLIAKTMTERLKVT 526
            FQ    +GI+ N   E  I LI K  K  ++ ++YRPISL     K++ K +T R++  
Sbjct: 498 LFQNIEKEGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQH 557

Query: 527 LPHTISDHQMAFVKERQ----ITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWS 586
           +   I   Q+ F+   Q    I  +I ++       K+K     ++ +D  KAFD I   
Sbjct: 558 IKKIIHHDQVGFIPGSQGWFNIRKSINVIQHIN---KLKNKDHMILSIDAEKAFDNIQHP 617

Query: 587 FIDYMLMKKNYSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLA 646
           F+   L K    G + + I +  S    +I++NG          G R+G PLSP +F + 
Sbjct: 618 FMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIV 677

Query: 647 MDYFSRLIRHVQQQGKIKGVCFNNE 663
           M+  +  IR   ++  IKG+   +E
Sbjct: 678 MEVLAIAIR---EEKAIKGIHIGSE 692

BLAST of CSPI06G21300 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.2e-20
Identity = 73/261 (27.97%), Postives = 125/261 (47.89%), Query Frame = 0

Query: 407 PPITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVD 466
           P +     + L  P   SEI + ++S    ++PGPD FT EF +++ + L   +  +F  
Sbjct: 439 PRLNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQS 498

Query: 467 FFQKGIINNIVNETYIALIAKKEK-CSKADDYRPISLRTALYKLIAKTMTERLKVTLPHT 526
             ++GI+ N   E  I LI K  +  +K +++RPISL     K++ K +  R++  +   
Sbjct: 499 IEKEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKL 558

Query: 527 ISDHQMAFVKERQ----ITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDY 586
           I   Q+ F+   Q    I  +I ++       + K     +I +D  KAFDKI   F+  
Sbjct: 559 IHHDQVGFIPGMQGWFNIRKSINVIQHIN---RAKDKNHVIISIDAEKAFDKIQQPFMLK 618

Query: 587 MLMKKNYSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYF 646
            L K    G + + I +       +I++NG+         G R+G PLSP +F + ++  
Sbjct: 619 TLNKLGIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVL 678

Query: 647 SRLIRHVQQQGKIKGVCFNNE 663
           +R IR   Q+ +IKG+    E
Sbjct: 679 ARAIR---QEKEIKGIQLGKE 693

BLAST of CSPI06G21300 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.9e-10
Identity = 29/63 (46.03%), Postives = 43/63 (68.25%), Query Frame = 0

Query: 608 LINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRHVQQQGKIKGV-CFNNEFNLT 667
           +ING P G + P+RG+R+GDPLSP++F+L  +  S L R  Q+QG++ G+   NN   + 
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 668 HLL 670
           HLL
Sbjct: 73  HLL 75

BLAST of CSPI06G21300 vs. ExPASy TrEMBL
Match: A0A5A7US62 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold280G003960 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 3.2e-93
Identity = 167/262 (63.74%), Postives = 207/262 (79.01%), Query Frame = 0

Query: 408  PITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDF 467
            PI+   + +LC PF ESEI ST+ SF+N + PGPD +T+ F KKHW  LK D+  VF DF
Sbjct: 1031 PISRLCQSELCKPFDESEIKSTIMSFSNEKAPGPDGYTMLFYKKHWPDLKDDLLNVFKDF 1090

Query: 468  FQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTIS 527
             + GI+NN VN T+IALI+KKEKCSK  DYRPISL T+LYK++AK +  RLK  LP TI+
Sbjct: 1091 HKAGIVNNNVNNTFIALISKKEKCSKPSDYRPISLTTSLYKIMAKALANRLKSALPDTIA 1150

Query: 528  DHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKN 587
            ++QMAF+K RQI DAILI NEAID WK +K +GFV+KLDI KAFDKI+WSFIDYML KK+
Sbjct: 1151 ENQMAFIKGRQINDAILIANEAIDTWKQRKIKGFVLKLDIEKAFDKISWSFIDYMLAKKH 1210

Query: 588  YSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRH 647
            +  +WR+WI +CIS+V YSIL+NG P G+IK  RGIR+GDPLSPFIFVLAMDY SRL+ H
Sbjct: 1211 FPHKWRKWIKACISNVQYSILLNGAPKGRIKAERGIRQGDPLSPFIFVLAMDYLSRLLSH 1270

Query: 648  VQQQGKIKGVCFNNEFNLTHLL 670
            ++ +G IKGV FNN  N++HLL
Sbjct: 1271 LESKGAIKGVSFNNCCNISHLL 1292

BLAST of CSPI06G21300 vs. ExPASy TrEMBL
Match: A0A5D3CA17 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00050 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 5.4e-93
Identity = 166/262 (63.36%), Postives = 206/262 (78.63%), Query Frame = 0

Query: 408  PITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDF 467
            PI+   + +LC PF ESEI ST+ SF+N + PGPD +T+ F KKHW  LK D+  VF DF
Sbjct: 1031 PISRLCQSELCKPFDESEIKSTIMSFSNEKAPGPDGYTMLFYKKHWPDLKDDLLNVFKDF 1090

Query: 468  FQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTIS 527
             + GI+NN VN T+IALI+KKEKCSK  DYRPISL T+LYK++AK +  RLK  LP TI+
Sbjct: 1091 HKAGIVNNNVNNTFIALISKKEKCSKPSDYRPISLTTSLYKIMAKALANRLKSALPDTIA 1150

Query: 528  DHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKN 587
            ++QMAF+K RQI DAILI NE ID WK +K +GFV+KLDI KAFDKI+WSFIDYML KK+
Sbjct: 1151 ENQMAFIKGRQINDAILIANEVIDTWKQRKIKGFVLKLDIEKAFDKISWSFIDYMLAKKH 1210

Query: 588  YSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRH 647
            +  +WR+WI +CIS+V YSIL+NG P G+IK  RGIR+GDPLSPFIFVLAMDY SRL+ H
Sbjct: 1211 FPHKWRKWIKACISNVQYSILLNGAPKGRIKAERGIRQGDPLSPFIFVLAMDYLSRLLSH 1270

Query: 648  VQQQGKIKGVCFNNEFNLTHLL 670
            ++ +G IKGV FNN  N++HLL
Sbjct: 1271 LESKGAIKGVSFNNYCNISHLL 1292

BLAST of CSPI06G21300 vs. ExPASy TrEMBL
Match: A0A5D3DM72 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002870 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 5.1e-91
Identity = 171/283 (60.42%), Postives = 212/283 (74.91%), Query Frame = 0

Query: 408 PITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDF 467
           PI+ +    LC  F E EIH  L++F+NN++PGPD FT+EF K  W +LK++I  +F DF
Sbjct: 265 PISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDGFTMEFYKSTWSVLKEEILNIFRDF 324

Query: 468 FQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTIS 527
               IIN  VN T IALIAKKEKC++  DYRPISL T++YKLIAK + ERLK TLP+T++
Sbjct: 325 HSNCIINKAVNITNIALIAKKEKCAEPADYRPISLTTSIYKLIAKVIAERLKDTLPYTVA 384

Query: 528 DHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKN 587
           ++QMAFVK RQI DAIL+ NEAIDYW+VKK +GFVIKLDI KAFDK+NW FID+MLMKK 
Sbjct: 385 ENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVIKLDIEKAFDKLNWRFIDFMLMKKG 444

Query: 588 YSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRH 647
           Y  +WR WI +CISSV YSI+ING+P GKI+P+RGIR+GDP+SPFIFVLAMDY SRL+  
Sbjct: 445 YPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMDYMSRLLNS 504

Query: 648 VQQQGKIKGVCFNNEFNLTHLLQS----IKVHHISHKCRQLQN 687
           V +  KIKGV      NLTHLL +    + V    H  + L+N
Sbjct: 505 VGE--KIKGVKLEGNINLTHLLFADDILLFVEDDEHSIQNLKN 545

BLAST of CSPI06G21300 vs. ExPASy TrEMBL
Match: A0A1S4E2K5 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC107991687 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 5.1e-91
Identity = 171/283 (60.42%), Postives = 212/283 (74.91%), Query Frame = 0

Query: 408 PITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDF 467
           PI+ +    LC  F E EIH  L++F+NN++PGPD FT+EF K  W +LK++I  +F DF
Sbjct: 265 PISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDGFTMEFYKSTWSVLKEEILNIFRDF 324

Query: 468 FQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTIS 527
               IIN  VN T IALIAKKEKC++  DYRPISL T++YKLIAK + ERLK TLP+T++
Sbjct: 325 HSNCIINKAVNITNIALIAKKEKCAEPADYRPISLTTSIYKLIAKVIAERLKDTLPYTVA 384

Query: 528 DHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKN 587
           ++QMAFVK RQI DAIL+ NEAIDYW+VKK +GFVIKLDI KAFDK+NW FID+MLMKK 
Sbjct: 385 ENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVIKLDIEKAFDKLNWRFIDFMLMKKG 444

Query: 588 YSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRH 647
           Y  +WR WI +CISSV YSI+ING+P GKI+P+RGIR+GDP+SPFIFVLAMDY SRL+  
Sbjct: 445 YPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMDYMSRLLNS 504

Query: 648 VQQQGKIKGVCFNNEFNLTHLLQS----IKVHHISHKCRQLQN 687
           V +  KIKGV      NLTHLL +    + V    H  + L+N
Sbjct: 505 VGE--KIKGVKLEGNINLTHLLFADDILLFVEDDEHSIQNLKN 545

BLAST of CSPI06G21300 vs. ExPASy TrEMBL
Match: A0A5A7T9I7 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00980 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.1e-90
Identity = 168/262 (64.12%), Postives = 205/262 (78.24%), Query Frame = 0

Query: 408 PITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDF 467
           PI++   + L  PF E+EI  TL SFA N+ PGPD + ++FL+K W  +KQ+I  +F DF
Sbjct: 351 PISNINSELLDKPFNEAEIWLTLKSFAKNKAPGPDGYAMDFLQKSWSFMKQNICDIFKDF 410

Query: 468 FQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTIS 527
               IIN +VNET I LIAKKE C  A D+RPISL TA+YKLIAKT+ +RLK TLP TIS
Sbjct: 411 HSTHIINKVVNETLITLIAKKEHCETAADFRPISLTTAIYKLIAKTLADRLKQTLPDTIS 470

Query: 528 DHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKN 587
           + QMAFVK RQIT+AILI NEA+D+W+ KK RGFVIKLDI KAFDK+NW FID++LMKKN
Sbjct: 471 ESQMAFVKGRQITEAILIANEALDFWRSKKERGFVIKLDIEKAFDKLNWRFIDFVLMKKN 530

Query: 588 YSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRH 647
           YS +WR+ I SCISSV YSILING+P G+IKP+RGIR+GDPLSPFIFVLAMDY SRL+ +
Sbjct: 531 YSQKWRKMIASCISSVQYSILINGRPRGRIKPSRGIRQGDPLSPFIFVLAMDYLSRLLNN 590

Query: 648 VQQQGKIKGVCFNNEFNLTHLL 670
           +  + KI GV F+   NLTH+L
Sbjct: 591 LADKRKINGVKFSPNLNLTHIL 612

BLAST of CSPI06G21300 vs. NCBI nr
Match: KAA0057507.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 352.8 bits (904), Expect = 6.6e-93
Identity = 167/262 (63.74%), Postives = 207/262 (79.01%), Query Frame = 0

Query: 408  PITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDF 467
            PI+   + +LC PF ESEI ST+ SF+N + PGPD +T+ F KKHW  LK D+  VF DF
Sbjct: 1031 PISRLCQSELCKPFDESEIKSTIMSFSNEKAPGPDGYTMLFYKKHWPDLKDDLLNVFKDF 1090

Query: 468  FQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTIS 527
             + GI+NN VN T+IALI+KKEKCSK  DYRPISL T+LYK++AK +  RLK  LP TI+
Sbjct: 1091 HKAGIVNNNVNNTFIALISKKEKCSKPSDYRPISLTTSLYKIMAKALANRLKSALPDTIA 1150

Query: 528  DHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKN 587
            ++QMAF+K RQI DAILI NEAID WK +K +GFV+KLDI KAFDKI+WSFIDYML KK+
Sbjct: 1151 ENQMAFIKGRQINDAILIANEAIDTWKQRKIKGFVLKLDIEKAFDKISWSFIDYMLAKKH 1210

Query: 588  YSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRH 647
            +  +WR+WI +CIS+V YSIL+NG P G+IK  RGIR+GDPLSPFIFVLAMDY SRL+ H
Sbjct: 1211 FPHKWRKWIKACISNVQYSILLNGAPKGRIKAERGIRQGDPLSPFIFVLAMDYLSRLLSH 1270

Query: 648  VQQQGKIKGVCFNNEFNLTHLL 670
            ++ +G IKGV FNN  N++HLL
Sbjct: 1271 LESKGAIKGVSFNNCCNISHLL 1292

BLAST of CSPI06G21300 vs. NCBI nr
Match: TYK08190.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 352.1 bits (902), Expect = 1.1e-92
Identity = 166/262 (63.36%), Postives = 206/262 (78.63%), Query Frame = 0

Query: 408  PITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDF 467
            PI+   + +LC PF ESEI ST+ SF+N + PGPD +T+ F KKHW  LK D+  VF DF
Sbjct: 1031 PISRLCQSELCKPFDESEIKSTIMSFSNEKAPGPDGYTMLFYKKHWPDLKDDLLNVFKDF 1090

Query: 468  FQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTIS 527
             + GI+NN VN T+IALI+KKEKCSK  DYRPISL T+LYK++AK +  RLK  LP TI+
Sbjct: 1091 HKAGIVNNNVNNTFIALISKKEKCSKPSDYRPISLTTSLYKIMAKALANRLKSALPDTIA 1150

Query: 528  DHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKN 587
            ++QMAF+K RQI DAILI NE ID WK +K +GFV+KLDI KAFDKI+WSFIDYML KK+
Sbjct: 1151 ENQMAFIKGRQINDAILIANEVIDTWKQRKIKGFVLKLDIEKAFDKISWSFIDYMLAKKH 1210

Query: 588  YSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRH 647
            +  +WR+WI +CIS+V YSIL+NG P G+IK  RGIR+GDPLSPFIFVLAMDY SRL+ H
Sbjct: 1211 FPHKWRKWIKACISNVQYSILLNGAPKGRIKAERGIRQGDPLSPFIFVLAMDYLSRLLSH 1270

Query: 648  VQQQGKIKGVCFNNEFNLTHLL 670
            ++ +G IKGV FNN  N++HLL
Sbjct: 1271 LESKGAIKGVSFNNYCNISHLL 1292

BLAST of CSPI06G21300 vs. NCBI nr
Match: XP_016902461.1 (PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo])

HSP 1 Score: 345.5 bits (885), Expect = 1.0e-90
Identity = 171/283 (60.42%), Postives = 212/283 (74.91%), Query Frame = 0

Query: 408 PITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDF 467
           PI+ +    LC  F E EIH  L++F+NN++PGPD FT+EF K  W +LK++I  +F DF
Sbjct: 265 PISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDGFTMEFYKSTWSVLKEEILNIFRDF 324

Query: 468 FQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTIS 527
               IIN  VN T IALIAKKEKC++  DYRPISL T++YKLIAK + ERLK TLP+T++
Sbjct: 325 HSNCIINKAVNITNIALIAKKEKCAEPADYRPISLTTSIYKLIAKVIAERLKDTLPYTVA 384

Query: 528 DHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKN 587
           ++QMAFVK RQI DAIL+ NEAIDYW+VKK +GFVIKLDI KAFDK+NW FID+MLMKK 
Sbjct: 385 ENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVIKLDIEKAFDKLNWRFIDFMLMKKG 444

Query: 588 YSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRH 647
           Y  +WR WI +CISSV YSI+ING+P GKI+P+RGIR+GDP+SPFIFVLAMDY SRL+  
Sbjct: 445 YPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMDYMSRLLNS 504

Query: 648 VQQQGKIKGVCFNNEFNLTHLLQS----IKVHHISHKCRQLQN 687
           V +  KIKGV      NLTHLL +    + V    H  + L+N
Sbjct: 505 VGE--KIKGVKLEGNINLTHLLFADDILLFVEDDEHSIQNLKN 545

BLAST of CSPI06G21300 vs. NCBI nr
Match: KAA0039770.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24727.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 345.5 bits (885), Expect = 1.0e-90
Identity = 171/283 (60.42%), Postives = 212/283 (74.91%), Query Frame = 0

Query: 408 PITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDF 467
           PI+ +    LC  F E EIH  L++F+NN++PGPD FT+EF K  W +LK++I  +F DF
Sbjct: 265 PISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDGFTMEFYKSTWSVLKEEILNIFRDF 324

Query: 468 FQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTIS 527
               IIN  VN T IALIAKKEKC++  DYRPISL T++YKLIAK + ERLK TLP+T++
Sbjct: 325 HSNCIINKAVNITNIALIAKKEKCAEPADYRPISLTTSIYKLIAKVIAERLKDTLPYTVA 384

Query: 528 DHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKN 587
           ++QMAFVK RQI DAIL+ NEAIDYW+VKK +GFVIKLDI KAFDK+NW FID+MLMKK 
Sbjct: 385 ENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVIKLDIEKAFDKLNWRFIDFMLMKKG 444

Query: 588 YSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRH 647
           Y  +WR WI +CISSV YSI+ING+P GKI+P+RGIR+GDP+SPFIFVLAMDY SRL+  
Sbjct: 445 YPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMDYMSRLLNS 504

Query: 648 VQQQGKIKGVCFNNEFNLTHLLQS----IKVHHISHKCRQLQN 687
           V +  KIKGV      NLTHLL +    + V    H  + L+N
Sbjct: 505 VGE--KIKGVKLEGNINLTHLLFADDILLFVEDDEHSIQNLKN 545

BLAST of CSPI06G21300 vs. NCBI nr
Match: TYK21642.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 344.4 bits (882), Expect = 2.3e-90
Identity = 170/283 (60.07%), Postives = 213/283 (75.27%), Query Frame = 0

Query: 408 PITDSLKDQLCLPFRESEIHSTLSSFANNETPGPDRFTIEFLKKHWKILKQDIKIVFVDF 467
           PI+ +    LC  F E EIH+ L++F+NN++PGPD FT+EF K  W +LK++I  +F DF
Sbjct: 89  PISTTQAQNLCSLFTEEEIHAALTAFSNNKSPGPDGFTMEFYKSTWSVLKEEIFNIFRDF 148

Query: 468 FQKGIINNIVNETYIALIAKKEKCSKADDYRPISLRTALYKLIAKTMTERLKVTLPHTIS 527
               IIN  VN T IALIAKKEKC++  DYRPISL T++YKLIAK + ERLK TLP T++
Sbjct: 149 HSNCIINKAVNMTNIALIAKKEKCAEPADYRPISLTTSIYKLIAKVIAERLKETLPSTVA 208

Query: 528 DHQMAFVKERQITDAILIVNEAIDYWKVKKTRGFVIKLDIAKAFDKINWSFIDYMLMKKN 587
           ++QMAFVK RQI DAIL+ NEAIDYW+VKK +GFVIKLDI KAFDK+NW FID+MLMKK 
Sbjct: 209 ENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVIKLDIEKAFDKLNWRFIDFMLMKKG 268

Query: 588 YSGQWRRWIHSCISSVHYSILINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRH 647
           Y  +WR+WI +CISSV YSI+ING+P GKI+P+RGIR+GDP+SPFIFVLAMDY SRL+  
Sbjct: 269 YPFRWRKWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMDYISRLLNS 328

Query: 648 VQQQGKIKGVCFNNEFNLTHLLQS----IKVHHISHKCRQLQN 687
           V +  KIKG+      NLTHLL +    + V    H  + L+N
Sbjct: 329 VGE--KIKGMKMEGNINLTHLLFADDILLFVEDDEHSIQNLKN 369

BLAST of CSPI06G21300 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 69.3 bits (168), Expect = 1.3e-11
Identity = 29/63 (46.03%), Postives = 43/63 (68.25%), Query Frame = 0

Query: 608 LINGKPSGKIKPTRGIRRGDPLSPFIFVLAMDYFSRLIRHVQQQGKIKGV-CFNNEFNLT 667
           +ING P G + P+RG+R+GDPLSP++F+L  +  S L R  Q+QG++ G+   NN   + 
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 668 HLL 670
           HLL
Sbjct: 73  HLL 75

BLAST of CSPI06G21300 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 52.4 bits (124), Expect = 1.7e-06
Identity = 28/81 (34.57%), Postives = 47/81 (58.02%), Query Frame = 0

Query: 514 MTERLKVTLPHTISDHQMAFVKERQITDAILIVNEAIDYWKVKK-TRGF-VIKLDIAKAF 573
           M ERLK  + + I   Q +F+  R  TD I+ V EA+   + KK  +G+ ++KLD+ KA+
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAY 60

Query: 574 DKINWSFIDYMLMKKNYSGQW 593
           D+I W +++  L+   +   W
Sbjct: 61  DRIRWDYLEDTLISAGFPEVW 81

BLAST of CSPI06G21300 vs. TAIR 10
Match: AT1G07660.1 (Histone superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 3.6e-04
Identity = 25/43 (58.14%), Postives = 29/43 (67.44%), Query Frame = 0

Query: 273 ERNSNVLKIFLKNFIRNIVTYTEHTLQKTDTTMDVAAKQYSLK 316
           E    VLKIFL+N IR+ VTYTEH  +KT T MDV    Y+LK
Sbjct: 53  EETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVV---YALK 92

BLAST of CSPI06G21300 vs. TAIR 10
Match: AT1G07820.1 (Histone superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 3.6e-04
Identity = 25/43 (58.14%), Postives = 29/43 (67.44%), Query Frame = 0

Query: 273 ERNSNVLKIFLKNFIRNIVTYTEHTLQKTDTTMDVAAKQYSLK 316
           E    VLKIFL+N IR+ VTYTEH  +KT T MDV    Y+LK
Sbjct: 53  EETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVV---YALK 92

BLAST of CSPI06G21300 vs. TAIR 10
Match: AT1G07820.2 (Histone superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 3.6e-04
Identity = 25/43 (58.14%), Postives = 29/43 (67.44%), Query Frame = 0

Query: 273 ERNSNVLKIFLKNFIRNIVTYTEHTLQKTDTTMDVAAKQYSLK 316
           E    VLKIFL+N IR+ VTYTEH  +KT T MDV    Y+LK
Sbjct: 53  EETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVV---YALK 92

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P113699.7e-2329.76LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
P143813.7e-2227.27Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
P085484.1e-2128.68LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
O003701.2e-2027.97LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P925551.9e-1046.03Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A5A7US623.2e-9363.74LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3CA175.4e-9363.36LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3DM725.1e-9160.42LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S4E2K55.1e-9160.42LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC1079... [more]
A0A5A7T9I71.1e-9064.12LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
KAA0057507.16.6e-9363.74LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
TYK08190.11.1e-9263.36LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
XP_016902461.11.0e-9060.42PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo][more]
KAA0039770.11.0e-9060.42LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK247... [more]
TYK21642.12.3e-9060.07LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
ATMG01250.11.3e-1146.03RNA-directed DNA polymerase (reverse transcriptase) [more]
AT4G20520.11.7e-0634.57RNA binding;RNA-directed DNA polymerases [more]
AT1G07660.13.6e-0458.14Histone superfamily protein [more]
AT1G07820.13.6e-0458.14Histone superfamily protein [more]
AT1G07820.23.6e-0458.14Histone superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001951Histone H4SMARTSM00417h44coord: 249..310
e-value: 0.0031
score: 26.7
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 487..648
e-value: 9.0E-31
score: 107.1
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 466..686
score: 8.916615
IPR009072Histone-foldGENE3D1.10.20.10Histone, subunit Acoord: 270..315
e-value: 1.1E-6
score: 30.8
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 417..655
NoneNo IPR availablePANTHERPTHR33116:SF38OS01G0158850 PROTEINcoord: 417..655
NoneNo IPR availableCDDcd01650RT_nLTR_likecoord: 479..669
e-value: 2.48241E-34
score: 128.563
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 430..646

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G21300.1CSPI06G21300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003677 DNA binding
molecular_function GO:0046982 protein heterodimerization activity