CSPI06G09570 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G09570
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	N-acyl-L-amino-acid amidohydrolase
Location	Chr6: 8184493 .. 8187566 (-)
RNA-Seq Expression	CSPI06G09570
Synteny	CSPI06G09570

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTGAATCGATGGCCCAGCTTTTGGTTACTGTTGAAGTGGGGGTTTTAGCTCAAATTTCATCATTATCCCATCTCCCCAATATCAATGGCAGAATGGACCTCTGTCGTCTTCTCTTAACCCCACCCTTTCTCTTCTCCATTCCAATTTCTCAATAGTTATTGAACTTAATTGTACCAGGGTCCTTCTGAATCTGAGCCATGAATTTCAAATCTCTCCATTTTCTCCTTTCCATTATAACTCTCTTCTTCTTTCTATCAATTTCTACTTCTGAACAGGACCCATCGTCTCCCTCTGCAATCATATCCAGGTTTCAACAATACCTTCAGATTAATACCGTCCAACCCAGCCCCCAATACTATGAGGCTGCTGATTTCATCATTTCTCAGGCCAAATCGCTCTCCCTTGAATCTCACACAATTGAATTCGTCGAGGGCAAGCCTCTGGTCATTCTCAAATGGCCTGGTTCCAACCCTGAACTCCCTTCCATTCTCCTCAATTCGCATACCGATGTTGTTCCAGCTGAGCATAAAAAATGGATGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCAAGAGGGTCTCAGGATATGAAGTGCGTTGGGATGCAGTACCTTGAGGCTATTCGGCGTTTGAAGGCATCTGGGTTTCAGCCTCTGCGCTCTGTTTATTTATCTTTTGTCCCGGATGAAGAAATTGGTGGCCATGGTGGTGCTGAGAAATTTGCTGAATCCGATGAGTTCAAGAAATTGAATGTCGCCATTGTGCTTGATGAAGGTTCCGAGTGCACCCCTTTTTGCTGATTTTTCTGATGGGTGAGTTTCTTTCATCCTTGTGTGCTTCGCATGTTTAGACTAATGTTGTTCGAAAATCATCAGGGTTGCCTTCTCCGGGTGAGAATTATAGGGTGTTTTATGGGGAAAAGAGCCCCTGGTGGTTGGTGATCAAGGCAGTGGGAGCCCCAGGTCATGGAGCTAGGCTTTATGATAACACTGCCTTGGAGAATCTTTTCAAAAGCATTGAGACTGTCAGAAGGTTTAGAGCTTCACAGTTTGATCTGATCAAAGGTGGTTTGAAGACTGAAGGAGATGTGGTCTCTGTCAACATGGTCTTCTTAAAATCTGGCATTCCATCTCCTACTGTGAGCTCATCTTTGAAACTTGTTTCTCCATGAATGCTTTTAGCTGTAATCCTTCTAGCACTAATAGATGAAACTATCATTCTTCATTTGAGTATTAGTTCAATCATTGTATCAGGCAATTCGGCTAGAATTTATTAGTATTGTCCTATTTTTCGTTTGAGTTCCAAGTTGAACTTCAAAAGCAATGGTTTTGACGGTTGGAATAATAGAATATTTGGGTTATTATATCAATCAAGAATGTCTTGGTTGAATTTTTTATGTTGGTTTTATCATGTTGGATGTCATGACAGACTATTTTGTTGATATTCTGTGTCATACAAAAGAATCTTTATGCTTATGAAATCCTGTTGCCATTATAACCTGATGGCTTACTCTACTTTTCTACCAGGGCTTTGTCATGAATCTGCAGCCATCTGAAGCTGAGGCTGGTTTTGACGTTCGCCTCCCACCGACTACAAATCCAGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCAGCCTCTCGTAATATGACGTTTGAGGTAACAATTTAGGGATCTTTTGATTCTGGCCTAAGAAGCGTGGAATACAATCACAGCATGTATTCTAAGGAGCAAAGACACAAATAATGGACATATATACTCGAATATATGTTATTATATCTAATTTTCAATGACTTTGCATTATAAATTCGTTTTTCTATGTTTAAAATGATAAAATGTGTTACAAATGCAGTGTAGAACATCTATCCAACAAGTGGTGGAGTGATGGATGTCCAATACACAGCGGACTCAAAACTCAAATATTTGCTTAAAAAATTCTTACCACTATGACTTGTACAGACTATGCTAATTCCACTATCACTTTTACAATGTAAATTCTTCCTCTCATATCTTTCCTTGTGCTTGATTGTTGCAGTTTAAGCAGAAGGAGTCTATTTACAACAAGTTTGGGAAACCAGCTCTTACTGCTATTGACAAATCCAATCCATGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCGAATGGAAAACTTGCAAACCCAGAAATCTTTCCTGCTTCAACGGATGCCCGATACTTTCGAAATCTTGGCCTCCCTGCAATCGGCTTCTCTCCAATGGTCAACACTCCTATTCTTCTTCATGACCATAATGAGGTGAGAATCTTATTCCTCCGTTCTTCAGTTCCAGCAGAAACTTTTAAGTTGGGTGGTAGCATAAATTTTTGTCATAGTTTGCCTTCGCTCAACTTTTTTACCGAAGGTTGAGATGTTGATTGATCATAGTAAATTAATTGATAAACTGAGCAGCTAAACCACTGTATTCCTTTACCTTCATTTTAATTTTTGGAGTTTTCGGATATGGATCTGGTTGGTCGTTTTTGTGGGCTGTCATTGAACTGCATTGTTTTCTAAGTCATTAATATGTTCATTTGTTCCAGTTTCTAAACCAAGCCGAGTATTTGAAAGGCATTGAGGTGTATGAGTCAATAATCAAGGCTTATGCTTCTTACGACGGTGACAAACCGATGGAGAGCTTCAAAGATGAGTTATAAGAAAGCAGCAAGGCAGTGATATCCTTTGAGCATTCAGCTACGAGGTTCGAATTTGCTCTGTAGGGATCAGGCAACTTCAGAGCATGGGGTTGGAAGGGATATGATTCTGTTCCCTAATTAATCTTCTGGAACATTACTTCATTGAATTTTGGTTGTGCTTTATTTGCAAGTTCCATATAATAATTCAGGCAGAAGAATTTTCTATCAAATAAAGTGACAACAGAATTGGCTTTGGAATAAACTCTTGTGTGTCATGGGGATTGGAATTTGTTAACCTGGGGATTTTGGAGGGATTAATTCTTAGAAGTTAATAAAATAAAATAGTTTCAAGTGAGAAAGAATTTGCTATTTTACTCTTTTGTCCTTATTTATTGCAGAAC

mRNA sequence

TGTGAATCGATGGCCCAGCTTTTGGTTACTGTTGAAGTGGGGGTTTTAGCTCAAATTTCATCATTATCCCATCTCCCCAATATCAATGGCAGAATGGACCTCTGTCGTCTTCTCTTAACCCCACCCTTTCTCTTCTCCATTCCAATTTCTCAATAGTTATTGAACTTAATTGTACCAGGGTCCTTCTGAATCTGAGCCATGAATTTCAAATCTCTCCATTTTCTCCTTTCCATTATAACTCTCTTCTTCTTTCTATCAATTTCTACTTCTGAACAGGACCCATCGTCTCCCTCTGCAATCATATCCAGGTTTCAACAATACCTTCAGATTAATACCGTCCAACCCAGCCCCCAATACTATGAGGCTGCTGATTTCATCATTTCTCAGGCCAAATCGCTCTCCCTTGAATCTCACACAATTGAATTCGTCGAGGGCAAGCCTCTGGTCATTCTCAAATGGCCTGGTTCCAACCCTGAACTCCCTTCCATTCTCCTCAATTCGCATACCGATGTTGTTCCAGCTGAGCATAAAAAATGGATGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCAAGAGGGTCTCAGGATATGAAGTGCGTTGGGATGCAGTACCTTGAGGCTATTCGGCGTTTGAAGGCATCTGGGTTTCAGCCTCTGCGCTCTGTTTATTTATCTTTTGTCCCGGATGAAGAAATTGGTGGCCATGGTGGTGCTGAGAAATTTGCTGAATCCGATGAGTTCAAGAAATTGAATGTCGCCATTGTGCTTGATGAAGGGTTGCCTTCTCCGGGTGAGAATTATAGGGTGTTTTATGGGGAAAAGAGCCCCTGGTGGTTGGTGATCAAGGCAGTGGGAGCCCCAGGTCATGGAGCTAGGCTTTATGATAACACTGCCTTGGAGAATCTTTTCAAAAGCATTGAGACTGTCAGAAGGTTTAGAGCTTCACAGTTTGATCTGATCAAAGGTGGTTTGAAGACTGAAGGAGATGTGGTCTCTGTCAACATGGTCTTCTTAAAATCTGGCATTCCATCTCCTACTGGCTTTGTCATGAATCTGCAGCCATCTGAAGCTGAGGCTGGTTTTGACGTTCGCCTCCCACCGACTACAAATCCAGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCAGCCTCTCGTAATATGACGTTTGAGTTTAAGCAGAAGGAGTCTATTTACAACAAGTTTGGGAAACCAGCTCTTACTGCTATTGACAAATCCAATCCATGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCGAATGGAAAACTTGCAAACCCAGAAATCTTTCCTGCTTCAACGGATGCCCGATACTTTCGAAATCTTGGCCTCCCTGCAATCGGCTTCTCTCCAATGGTCAACACTCCTATTCTTCTTCATGACCATAATGAGTTTCTAAACCAAGCCGAGTATTTGAAAGGCATTGAGGTGTATGAGTCAATAATCAAGGCTTATGCTTCTTACGACGGTGACAAACCGATGGAGAGCTTCAAAGATGAGTTATAAGAAAGCAGCAAGGCAGTGATATCCTTTGAGCATTCAGCTACGAGGTTCGAATTTGCTCTGTAGGGATCAGGCAACTTCAGAGCATGGGGTTGGAAGGGATATGATTCTGTTCCCTAATTAATCTTCTGGAACATTACTTCATTGAATTTTGGTTGTGCTTTATTTGCAAGTTCCATATAATAATTCAGGCAGAAGAATTTTCTATCAAATAAAGTGACAACAGAATTGGCTTTGGAATAAACTCTTGTGTGTCATGGGGATTGGAATTTGTTAACCTGGGGATTTTGGAGGGATTAATTCTTAGAAGTTAATAAAATAAAATAGTTTCAAGTGAGAAAGAATTTGCTATTTTACTCTTTTGTCCTTATTTATTGCAGAAC

Coding sequence (CDS)

ATGAATTTCAAATCTCTCCATTTTCTCCTTTCCATTATAACTCTCTTCTTCTTTCTATCAATTTCTACTTCTGAACAGGACCCATCGTCTCCCTCTGCAATCATATCCAGGTTTCAACAATACCTTCAGATTAATACCGTCCAACCCAGCCCCCAATACTATGAGGCTGCTGATTTCATCATTTCTCAGGCCAAATCGCTCTCCCTTGAATCTCACACAATTGAATTCGTCGAGGGCAAGCCTCTGGTCATTCTCAAATGGCCTGGTTCCAACCCTGAACTCCCTTCCATTCTCCTCAATTCGCATACCGATGTTGTTCCAGCTGAGCATAAAAAATGGATGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCAAGAGGGTCTCAGGATATGAAGTGCGTTGGGATGCAGTACCTTGAGGCTATTCGGCGTTTGAAGGCATCTGGGTTTCAGCCTCTGCGCTCTGTTTATTTATCTTTTGTCCCGGATGAAGAAATTGGTGGCCATGGTGGTGCTGAGAAATTTGCTGAATCCGATGAGTTCAAGAAATTGAATGTCGCCATTGTGCTTGATGAAGGGTTGCCTTCTCCGGGTGAGAATTATAGGGTGTTTTATGGGGAAAAGAGCCCCTGGTGGTTGGTGATCAAGGCAGTGGGAGCCCCAGGTCATGGAGCTAGGCTTTATGATAACACTGCCTTGGAGAATCTTTTCAAAAGCATTGAGACTGTCAGAAGGTTTAGAGCTTCACAGTTTGATCTGATCAAAGGTGGTTTGAAGACTGAAGGAGATGTGGTCTCTGTCAACATGGTCTTCTTAAAATCTGGCATTCCATCTCCTACTGGCTTTGTCATGAATCTGCAGCCATCTGAAGCTGAGGCTGGTTTTGACGTTCGCCTCCCACCGACTACAAATCCAGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCAGCCTCTCGTAATATGACGTTTGAGTTTAAGCAGAAGGAGTCTATTTACAACAAGTTTGGGAAACCAGCTCTTACTGCTATTGACAAATCCAATCCATGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCGAATGGAAAACTTGCAAACCCAGAAATCTTTCCTGCTTCAACGGATGCCCGATACTTTCGAAATCTTGGCCTCCCTGCAATCGGCTTCTCTCCAATGGTCAACACTCCTATTCTTCTTCATGACCATAATGAGTTTCTAAACCAAGCCGAGTATTTGAAAGGCATTGAGGTGTATGAGTCAATAATCAAGGCTTATGCTTCTTACGACGGTGACAAACCGATGGAGAGCTTCAAAGATGAGTTATAA

Protein sequence

MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESIIKAYASYDGDKPMESFKDEL*

Homology

[Close]

BLAST of CSPI06G09570 vs. ExPASy Swiss-Prot
Match: Q99JW2 (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 8.9e-102
Identity = 185/411 (45.01%), Postives = 257/411 (62.53%), Query Frame = 0


Query: 26  QDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLVIL 85
           +DP S    ++ F+QYL+I TVQP+P Y  A  F+  +A+ L L    IE V G  + +L
Sbjct: 4   KDPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVL 63

Query: 86  KWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGAHIDSHGNIYARGSQDMKCVGMQYLE 145
            WPG+NP LPSILLNSHTDVVP   + W H P  A  DS G IYARGSQDMK V +QYLE
Sbjct: 64  TWPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLE 123

Query: 146 AIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPGEN 205
           A+RRLK+ G +  R+++++FVPDEE+GGH G E F +  EF+ L     LDEGL +P + 
Sbjct: 124 AVRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDA 183

Query: 206 YRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKGG--L 265
           + VFY E+SPWW+ + + G PGH +R  ++TA E L K I ++  FR  +   ++    L
Sbjct: 184 FTVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHL 243

Query: 266 KTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEWAP 325
           K EG V SVN+  L+ G+        N+ P+   A FD R+ P  + ++ E+++      
Sbjct: 244 K-EGAVTSVNLTKLEGGV------AYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQE 303

Query: 326 ASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDA 385
           A   +TFEF Q      KF +P +T  D S+PWW     A +  N  L  PEIFPA+TD+
Sbjct: 304 AGEGVTFEFAQ------KFTEPRMTPTDDSDPWWAAFSGACKAMNLTL-EPEIFPAATDS 363

Query: 386 RYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 435
           RY R +G+PA+GFSPM  TP+LLHDHNE L++  +L+G+++Y  ++ A AS
Sbjct: 364 RYIRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

[Close]

BLAST of CSPI06G09570 vs. ExPASy Swiss-Prot
Match: Q6AYS7 (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.4e-99
Identity = 180/409 (44.01%), Postives = 254/409 (62.10%), Query Frame = 0


Query: 28  PSSPSAIISRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLVILKW 87
           P S    ++ F+QYL+I TVQP+P Y  A  F+  +A+ L L    IE   G  + +L W
Sbjct: 6   PESEHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTW 65

Query: 88  PGSNPELPSILLNSHTDVVPAEHKKWMHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAI 147
           PG+NP L SILLNSHTDVVP   + W H P  A  DS G IYARG+QDMK V +QYLEA+
Sbjct: 66  PGTNPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAV 125

Query: 148 RRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPGENYR 207
           RRLK+ G +  R+++++FVPDEE+GGH G E F +  EF+ L     LDEGL +P + + 
Sbjct: 126 RRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFT 185

Query: 208 VFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKGG--LKT 267
           VFY E+SPWW+ + + G PGH +R  ++TA E L K + ++  FR  +   ++    LK 
Sbjct: 186 VFYSERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLK- 245

Query: 268 EGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEWAPAS 327
           EG V SVN+  L+ G+        N+ P+   A FD R+ P  + ++ E+++      A 
Sbjct: 246 EGAVTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAG 305

Query: 328 RNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDARY 387
             +TFEF Q      KF +P +T  D ++PWW     A +  N  L  PEIFPA+TD+RY
Sbjct: 306 EGVTFEFAQ------KFTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDSRY 365

Query: 388 FRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 435
            R +G+PA+GFSPM  TP+LLHDHNE L++A +L+G+++Y  ++ A AS
Sbjct: 366 IRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

[Close]

BLAST of CSPI06G09570 vs. ExPASy Swiss-Prot
Match: Q55DP8 (Aminoacylase-1 OS=Dictyostelium discoideum OX=44689 GN=acy1 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 7.1e-99
Identity = 181/402 (45.02%), Postives = 260/402 (64.68%), Query Frame = 0


Query: 35  ISRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLVILKWPGSNPEL 94
           ++ F+++L+I T  P+P Y  +  F++ +AK  ++  + +    G P+V++K  G  P L
Sbjct: 10  VTVFREFLKIRTDHPTPDYESSTKFLVEKAKEYNI-PYEVYRETGTPIVLMKIEGLEPNL 69

Query: 95  PSILLNSHTDVVPAEHKKWMHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASG 154
            ++LLNSH DVVPA H  W   P  A  D  GNI+ RG+QDMKCV MQ+LE  RR+  SG
Sbjct: 70  KTVLLNSHVDVVPAVHDSWKVDPFSAWKDESGNIFGRGTQDMKCVCMQFLEVARRIVQSG 129

Query: 155 FQPLRSVYLSFVPDEEIGGHG-GAEKFAESDEFKKLNVAIVLDEGLPSPGENYRVFYGEK 214
            +  R+++LSFVPDEEIGG G G EKF  +++F++LN+ + LDEGL SP  ++ VFYGE+
Sbjct: 130 QKLKRTLHLSFVPDEEIGGSGKGMEKFVYTEKFRQLNIGLCLDEGLASPTNDFTVFYGER 189

Query: 215 SPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKGGL----KTEGDV 274
           +PWW+ I AVG  GHG+R  + TA+E L ++I  +  FR  QF+ +  G     K  GDV
Sbjct: 190 APWWVHITAVGNAGHGSRFIEGTAIEKLMRTINKMLAFRQEQFESLHHGQHECGKKLGDV 249

Query: 275 VSVNMVFLKSGIP--SPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEWAPASRN 334
            S+N+  LK+GIP      F  N+ P++AEAGFD+R+PPT N +    +I +EW  A   
Sbjct: 250 TSLNLTVLKAGIPIDHSNNFSYNVIPTQAEAGFDIRIPPTVNLDQFLDQI-KEWT-AEEG 309

Query: 335 MTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPASTDARYFR 394
           ++F+F       +   K  +T +D  N WW   +E+ +  +  L   EIFPA+TD+R+ R
Sbjct: 310 LSFKFA------SYIPKNEMTKLDSDNKWWENFKESCKKMDINLVT-EIFPAATDSRFIR 369

Query: 395 NLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESII 430
           NLG+PA GFSP+ NTPILLHDHNEFLN+  YL+GI+++  II
Sbjct: 370 NLGIPAFGFSPINNTPILLHDHNEFLNEKVYLRGIDIFMGII 401

[Close]

BLAST of CSPI06G09570 vs. ExPASy Swiss-Prot
Match: Q6PTT0 (Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 2.1e-98
Identity = 176/413 (42.62%), Postives = 249/413 (60.29%), Query Frame = 0


Query: 24  SEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLV 83
           + + P      ++ F+QYL+I TVQP P Y  A  F    A+ L L    +E   G  + 
Sbjct: 2   TSKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVT 61

Query: 84  ILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGAHIDSHGNIYARGSQDMKCVGMQY 143
           +L WPG+NP L SILLNSHTDVVP   + W H P  A  DS G IY RG+QDMKCV +QY
Sbjct: 62  VLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYTRGAQDMKCVSIQY 121

Query: 144 LEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPG 203
           LEA++RLK  G +  R+++++FVPDEE+GGH G E F +  EF  L     LDEGL +P 
Sbjct: 122 LEAVKRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRHEFHALRAGFALDEGLANPT 181

Query: 204 ENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKGG- 263
           + + VFY E+SPWW+ + + G PGH +R  ++TA E L K + ++  FR  ++  ++   
Sbjct: 182 DAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNP 241

Query: 264 -LKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEW 323
            LK EG V SVN+  L+ G+        N+ P+   A FD R+ P  + ++ E ++    
Sbjct: 242 HLK-EGSVTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEEQLQSWC 301

Query: 324 APASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPAST 383
             A   +TFEF Q      KF +P +T  D ++PWW     A +     L  PEIFPA+T
Sbjct: 302 QEAGEGVTFEFAQ------KFTEPRMTPTDDTDPWWAAFSGACKEMTLTL-EPEIFPAAT 361

Query: 384 DARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 435
           D+RY R +G+PA+GFSPM  TP+LLHDHNE L++A +L+G+++Y  ++ A AS
Sbjct: 362 DSRYIRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

[Close]

BLAST of CSPI06G09570 vs. ExPASy Swiss-Prot
Match: Q03154 (Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 6.6e-97
Identity = 175/413 (42.37%), Postives = 247/413 (59.81%), Query Frame = 0


Query: 24  SEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFIISQAKSLSLESHTIEFVEGKPLV 83
           + + P      ++ F+QYL+I TVQP P Y  A  F    A+ L L    +E   G  + 
Sbjct: 2   TSKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVT 61

Query: 84  ILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGAHIDSHGNIYARGSQDMKCVGMQY 143
           +L WPG+NP L SILLNSHTDVVP   + W H P  A  DS G IYARG+QDMKCV +QY
Sbjct: 62  VLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQY 121

Query: 144 LEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPG 203
           LEA+RRLK  G +  R+++++FVPDEE+GGH G E F +  EF  L     LDEG+ +P 
Sbjct: 122 LEAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPT 181

Query: 204 ENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETVRRFRASQFDLIKGG- 263
           + + VFY E+SPWW+ + + G PGH +R  ++TA E L K + ++  FR  ++  ++   
Sbjct: 182 DAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNP 241

Query: 264 -LKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTTNPESLERRIAEEW 323
            LK EG V SVN+  L+ G+        N+ P+   A FD R+ P  + ++ E ++    
Sbjct: 242 HLK-EGSVTSVNLTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWC 301

Query: 324 APASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNANGKLANPEIFPAST 383
             A   +T EF Q      K+  P +T  D SNPWW       ++ N  L  PEI PA+T
Sbjct: 302 QAAGEGVTLEFAQ------KWMHPQVTPTDDSNPWWAAFSRVCKDMNLTL-EPEIMPAAT 361

Query: 384 DARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 435
           D RY R +G+PA+GFSPM  TP+LLHDH+E L++A +L+G+++Y  ++ A AS
Sbjct: 362 DNRYIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALAS 400

[Close]

BLAST of CSPI06G09570 vs. ExPASy TrEMBL
Match: A0A0A0KAV8 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G117740 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 1.8e-259
Identity = 444/448 (99.11%), Postives = 445/448 (99.33%), Query Frame = 0


Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKW HPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIK GLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 360
           DVR+PPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNP WNLL
Sbjct: 301 DVRVPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPRWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 449
           GIEVYESIIKAYASYDGDKPMESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 448

[Close]

BLAST of CSPI06G09570 vs. ExPASy TrEMBL
Match: A0A5A7TXA6 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G00610 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.3e-257
Identity = 439/448 (97.99%), Postives = 443/448 (98.88%), Query Frame = 0


Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAI+SRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPL+ILKWPGSN +LPSILLNSHTDVVPAEH KW HPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIK GLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 360
           DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 449
           GIEVYESIIKAYASYDGDK MESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

[Close]

BLAST of CSPI06G09570 vs. ExPASy TrEMBL
Match: A0A5D3BSI0 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G00590 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 6.6e-257
Identity = 439/448 (97.99%), Postives = 443/448 (98.88%), Query Frame = 0


Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQ+PSSPSAIISRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPL+ILKWPGSN +LPSILLNSHTDVVPAEH KW HPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIK GLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 360
           DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 449
           GIEVYESIIKAYASYDGDK MESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

[Close]

BLAST of CSPI06G09570 vs. ExPASy TrEMBL
Match: A0A1S3BJ13 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo OX=3656 GN=LOC103490443 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 6.6e-257
Identity = 439/448 (97.99%), Postives = 443/448 (98.88%), Query Frame = 0


Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQ+PSSPSAIISRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPL+ILKWPGSN +LPSILLNSHTDVVPAEH KW HPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIK GLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 360
           DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 449
           GIEVYESIIKAYASYDGDK MESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

[Close]

BLAST of CSPI06G09570 vs. ExPASy TrEMBL
Match: A0A6J1CII7 (N-acyl-L-amino-acid amidohydrolase OS=Momordica charantia OX=3673 GN=LOC111011275 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 4.7e-231
Identity = 391/448 (87.28%), Postives = 416/448 (92.86%), Query Frame = 0


Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60
           MN K ++ LLS+IT+F FLS S ++++ SS SAII+RFQQYLQINTVQP+P YYEAADFI
Sbjct: 1   MNLKYVNLLLSVITIFLFLSSSIADEESSSSSAIIARFQQYLQINTVQPNPHYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGA 120
           ISQAKSL+LES TIEFVEGKPL++LKWPGSNP+L SILLNSHTDVVPAEH KW HPPLGA
Sbjct: 61  ISQAKSLALESQTIEFVEGKPLILLKWPGSNPDLSSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDS GNIYARGSQDMKCVGMQYLEAIRRLKA GF+P RSVYLSFVPDEEIGG  GAEKF
Sbjct: 121 HIDSLGNIYARGSQDMKCVGMQYLEAIRRLKALGFEPARSVYLSFVPDEEIGGRAGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEF+KLNV IVLDEGLPSPGENYRVFYGE+SPWW+VIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFQKLNVGIVLDEGLPSPGENYRVFYGERSPWWMVIKAVGAPGHGAKLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIE+VRRFRASQFDL+K GLK EG+VVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIESVRRFRASQFDLVKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 360
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKESI++KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESIFDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKL  PEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 449
           GIE YESIIKAYAS+   KP ESFKDEL
Sbjct: 421 GIEAYESIIKAYASFIAHKPTESFKDEL 448

[Close]

BLAST of CSPI06G09570 vs. NCBI nr
Match: XP_004139978.1 (aminoacylase-1 [Cucumis sativus] >KGN46643.1 hypothetical protein Csa_005514 [Cucumis sativus])

HSP 1 Score: 904.4 bits (2336), Expect = 3.8e-259
Identity = 444/448 (99.11%), Postives = 445/448 (99.33%), Query Frame = 0


Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKW HPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIK GLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 360
           DVR+PPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNP WNLL
Sbjct: 301 DVRVPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPRWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 449
           GIEVYESIIKAYASYDGDKPMESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 448

[Close]

BLAST of CSPI06G09570 vs. NCBI nr
Match: KAA0047810.1 (aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 897.5 bits (2318), Expect = 4.7e-257
Identity = 439/448 (97.99%), Postives = 443/448 (98.88%), Query Frame = 0


Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAI+SRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPL+ILKWPGSN +LPSILLNSHTDVVPAEH KW HPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIK GLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 360
           DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 449
           GIEVYESIIKAYASYDGDK MESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

[Close]

BLAST of CSPI06G09570 vs. NCBI nr
Match: XP_008448158.1 (PREDICTED: aminoacylase-1 [Cucumis melo] >TYK01049.1 aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 896.0 bits (2314), Expect = 1.4e-256
Identity = 439/448 (97.99%), Postives = 443/448 (98.88%), Query Frame = 0


Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60
           MNFKSLHFLLSIITLFFFLSISTSEQ+PSSPSAIISRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGA 120
           ISQAKSLSLESHTIEFVEGKPL+ILKWPGSN +LPSILLNSHTDVVPAEH KW HPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIETVRRFRASQFDLIK GLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 360
           DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIY+KFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 449
           GIEVYESIIKAYASYDGDK MESFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

[Close]

BLAST of CSPI06G09570 vs. NCBI nr
Match: XP_038876663.1 (aminoacylase-1 [Benincasa hispida])

HSP 1 Score: 866.7 bits (2238), Expect = 8.8e-248
Identity = 420/448 (93.75%), Postives = 432/448 (96.43%), Query Frame = 0


Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60
           M FKSLHFLLS ITLF  LS+STSE+DPSS SAIISRFQQYLQINTVQPSPQYYEAADFI
Sbjct: 1   MIFKSLHFLLSFITLFLLLSVSTSEEDPSSTSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGA 120
           ISQAK+LSLES TIEFVEGKPL++LKWPG NP+LPSILLNSHTDVVPAEH KW HPPLGA
Sbjct: 61  ISQAKALSLESQTIEFVEGKPLILLKWPGLNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGF+PLRSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFEPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           AESD F KLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGA+LYDN ALEN
Sbjct: 181 AESDVFNKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNAALEN 240

Query: 241 LFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           LFKSIE++RRFRASQFDLIK GLKTEGDVVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIESIRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 360
           DVRLPPT NPESLERRIAEEWAPASRNMTFEFKQKES+YNKFGKPALTAIDKSNPWWNLL
Sbjct: 301 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESVYNKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 449
           GIEVY+SIIKAYASYDGDKP ESFKDEL
Sbjct: 421 GIEVYKSIIKAYASYDGDKPTESFKDEL 448

[Close]

BLAST of CSPI06G09570 vs. NCBI nr
Match: XP_023532856.1 (aminoacylase-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 814.7 bits (2103), Expect = 4.0e-232
Identity = 397/449 (88.42%), Postives = 418/449 (93.10%), Query Frame = 0


Query: 1   MNFKSL-HFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADF 60
           MNFKSL H LLSI TLF FLSIS +E+DP S S+I+SRFQ+YLQINTVQP+PQYYEAADF
Sbjct: 1   MNFKSLNHLLLSITTLFLFLSISAAEEDPLSSSSIVSRFQRYLQINTVQPNPQYYEAADF 60

Query: 61  IISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLG 120
           IISQAKSLSLE HT+EFV GKPLV+LKWPGSNP LPSILLNSHTDVVP EH KW HPPLG
Sbjct: 61  IISQAKSLSLEYHTVEFVVGKPLVLLKWPGSNPNLPSILLNSHTDVVPFEHHKWTHPPLG 120

Query: 121 AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEK 180
           AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGF+P+RSVYLSFVPDEEIGGHGGAEK
Sbjct: 121 AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFEPVRSVYLSFVPDEEIGGHGGAEK 180

Query: 181 FAESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALE 240
           FAESDEFKKLNVAIVLDEGLPSPGENYR FYGE+SPWWLVIKAVGAPGHGA+LYDN ALE
Sbjct: 181 FAESDEFKKLNVAIVLDEGLPSPGENYRAFYGERSPWWLVIKAVGAPGHGAKLYDNAALE 240

Query: 241 NLFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAG 300
           NLFKSIE+VRRFRASQFDL+K GLK EG+VVSVNMV LK+G PSPTGFVMNLQPSEAEAG
Sbjct: 241 NLFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVSLKAGTPSPTGFVMNLQPSEAEAG 300

Query: 301 FDVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNL 360
           FDVRLPPT +PESLERRIAEEWAP SRNMTFEFKQKESIY+KFGKPALTAID SNPWWNL
Sbjct: 301 FDVRLPPTADPESLERRIAEEWAPVSRNMTFEFKQKESIYDKFGKPALTAIDSSNPWWNL 360

Query: 361 LEEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYL 420
           LEEAVRNANGKL NPEIFPASTDARYFRN GLPAIGFSPM NTP LLHDHNEFLNQAEYL
Sbjct: 361 LEEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPSLLHDHNEFLNQAEYL 420

Query: 421 KGIEVYESIIKAYASYDGDKPMESFKDEL 449
           KGIEVYESIIK+YASY G    ++FKDEL
Sbjct: 421 KGIEVYESIIKSYASYVG---RDNFKDEL 446

[Close]

BLAST of CSPI06G09570 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 648.3 bits (1671), Expect = 4.6e-186
Identity = 313/428 (73.13%), Postives = 360/428 (84.11%), Query Frame = 0


Query: 9   LLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFIISQAKSLS 68
           LL ++ +   L +S    D     AI+SRFQ+YL+INTVQP+P+YY+A DFIISQAK LS
Sbjct: 3   LLRLLLVVVVLHLSAVAGD----DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLS 62

Query: 69  LESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGAHIDSHGNI 128
           LES TIEFV+GKPL++LKW GS+P LP+ LLNSHTDVVP E  KW H PL AH+D HG+I
Sbjct: 63  LESQTIEFVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDI 122

Query: 129 YARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKK 188
           YARGSQDMKCVGMQYLEAIR+L+ASGF+PLRSVYLSFVPDEEIGGH GAEKFAES  FK 
Sbjct: 123 YARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKS 182

Query: 189 LNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETV 248
           LN+AIVLDEGLPSP E+YRVFYGE+SPWWLVIKA G PGHGA+LYDN+A+ENL KSIE++
Sbjct: 183 LNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESI 242

Query: 249 RRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTT 308
           RRFRASQFDL+K G   EGDVVSVNM FLK+G PSPTGFVMNLQPSEAEAGFD+R+PP+ 
Sbjct: 243 RRFRASQFDLLKAGGIAEGDVVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSV 302

Query: 309 NPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVRNAN 368
           + E+LERR+ EEWAPA+RNM+FEFKQK +     GK  LTA D SNPWW LLE AV+ A 
Sbjct: 303 DAEALERRLVEEWAPAARNMSFEFKQKLT-----GKQFLTAADDSNPWWGLLENAVKEAG 362

Query: 369 GKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYESI 428
           G+ + PEIFPASTDARYFR  G+PA GFSP+ NTP LLHDHNE+L +AEYLKGIEVY SI
Sbjct: 363 GRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSI 421

Query: 429 IKAYASYD 437
           IKAYASY+
Sbjct: 423 IKAYASYE 421

[Close]

BLAST of CSPI06G09570 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 642.9 bits (1657), Expect = 1.9e-184
Identity = 313/431 (72.62%), Postives = 360/431 (83.53%), Query Frame = 0


Query: 9   LLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFIISQAKSLS 68
           LL ++ +   L +S    D     AI+SRFQ+YL+INTVQP+P+YY+A DFIISQAK LS
Sbjct: 3   LLRLLLVVVVLHLSAVAGD----DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLS 62

Query: 69  LESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGAHIDSHGNI 128
           LES TIEFV+GKPL++LKW GS+P LP+ LLNSHTDVVP E  KW H PL AH+D HG+I
Sbjct: 63  LESQTIEFVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDI 122

Query: 129 YARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDEFKK 188
           YARGSQDMKCVGMQYLEAIR+L+ASGF+PLRSVYLSFVPDEEIGGH GAEKFAES  FK 
Sbjct: 123 YARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKS 182

Query: 189 LNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSIETV 248
           LN+AIVLDEGLPSP E+YRVFYGE+SPWWLVIKA G PGHGA+LYDN+A+ENL KSIE++
Sbjct: 183 LNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESI 242

Query: 249 RRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLPPTT 308
           RRFRASQFDL+K G   EGDVVSVNM FLK+G PSPTGFVMNLQPSEAEAGFD+R+PP+ 
Sbjct: 243 RRFRASQFDLLKAGGIAEGDVVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSV 302

Query: 309 NPESLERRIAEEWAPASRNMTFE---FKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVR 368
           + E+LERR+ EEWAPA+RNM+FE   FKQK +     GK  LTA D SNPWW LLE AV+
Sbjct: 303 DAEALERRLVEEWAPAARNMSFELGQFKQKLT-----GKQFLTAADDSNPWWGLLENAVK 362

Query: 369 NANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVY 428
            A G+ + PEIFPASTDARYFR  G+PA GFSP+ NTP LLHDHNE+L +AEYLKGIEVY
Sbjct: 363 EAGGRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVY 422

Query: 429 ESIIKAYASYD 437
            SIIKAYASY+
Sbjct: 423 VSIIKAYASYE 424

[Close]

BLAST of CSPI06G09570 vs. TAIR 10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 527.3 bits (1357), Expect = 1.2e-149
Identity = 238/438 (54.34%), Postives = 322/438 (73.52%), Query Frame = 0


Query: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60
           M    L + L I ++ F L  S+SE+D       I+RFQQYL+ NT  P+P Y     F+
Sbjct: 1   MAISPLLWTLIIFSIIFSLQSSSSEEDTP-----ITRFQQYLRFNTAHPNPNYTAPISFL 60

Query: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGA 120
           I+QA+S+ L + TIEF+ GKP++++ W GSNP LPSIL NSH D VPAE +KW +PP  A
Sbjct: 61  INQAQSIGLTTKTIEFISGKPILLITWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180
           H    G+IYARG+QD KC+G+QYLE+IR LK+ GF PLR++++S+VP+EEIGG  G  KF
Sbjct: 121 HKTIDGHIYARGAQDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240
           A S EFK LN+   +DEG  +PG+ +RVFY ++ PW  VIKA G PGHGA+LYDN+A+EN
Sbjct: 181 AASSEFKDLNLGFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMEN 240

Query: 241 LFKSIETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300
           L KS+E + RFR SQFD +K G     +V+SVN V+LK+G P+ TGFVMN+QPSEAEAG+
Sbjct: 241 LMKSVELISRFRESQFDFVKAGKAAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGY 300

Query: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLL 360
           D+RLPP  +P+ +++RIAEEWAP+ RNMT+  ++K  + +  G+P +T ++ SNPWW++ 
Sbjct: 301 DLRLPPMADPDVMKKRIAEEWAPSIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIF 360

Query: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420
           ++AV    GKLA PEI  ++TD+R+ R LG+P  GFSPM NTPILLHDHNEFL    ++K
Sbjct: 361 KQAVEAMGGKLAKPEILASTTDSRFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMK 420

Query: 421 GIEVYESIIKAYASYDGD 439
           GIEVYES+I A +S++G+
Sbjct: 421 GIEVYESVISALSSFEGE 433

[Close]

BLAST of CSPI06G09570 vs. TAIR 10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 520.0 bits (1338), Expect = 1.9e-147
Identity = 236/431 (54.76%), Postives = 317/431 (73.55%), Query Frame = 0


Query: 6   LHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFIISQAK 65
           L + L + +  F L   + E++  +P   I+RFQQYL+ NT  P+P Y     F++ QA+
Sbjct: 6   LLWTLFLFSFIFSLQSHSKEEEEDTP---ITRFQQYLRFNTAHPNPNYTAPISFLLDQAR 65

Query: 66  SLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGAHIDSH 125
           S+ L S TIEFV GKP+++L W GSN  L SIL NSH D VPAE +KW+HPP  AH    
Sbjct: 66  SIGLTSRTIEFVPGKPVLLLTWLGSNLNLSSILFNSHLDSVPAESEKWIHPPFSAHRTID 125

Query: 126 GNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKFAESDE 185
           G+IYARG+QD KC+G+QYLEAIR LK+  F PLR++++S+VP+EEIGG GG  KFA S E
Sbjct: 126 GHIYARGAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAASSE 185

Query: 186 FKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALENLFKSI 245
           FK LN+  V+DEG  SPG+ +RVFY E++PW L I+A G PGHGA+LYDN+A+ENL KS+
Sbjct: 186 FKDLNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAMENLMKSV 245

Query: 246 ETVRRFRASQFDLIKGGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGFDVRLP 305
           E + RFR SQFD +K G     +V+SVN V+LK+G PS TGFVMN+QPSEAE G+D+RLP
Sbjct: 246 ELISRFRESQFDFVKAGKAANSEVISVNPVYLKAGTPSTTGFVMNMQPSEAEVGYDLRLP 305

Query: 306 PTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPWWNLLEEAVR 365
           P  +P +L++RIAEEWAP+ RNMT+  KQ+  + +  G+P +T  + +NPWW++ ++AV 
Sbjct: 306 PMADPVALKKRIAEEWAPSIRNMTYTLKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQAVE 365

Query: 366 NANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVY 425
              GKLA PEI  ++TD+R+ R LG+P +GFSPM+NTPIL+HDHNEFL    ++KGI VY
Sbjct: 366 ATGGKLAKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVHDHNEFLKDTVFMKGIGVY 425

Query: 426 ESIIKAYASYD 437
           ES+I A +S++
Sbjct: 426 ESVISALSSFE 433

[Close]

BLAST of CSPI06G09570 vs. TAIR 10
Match: AT5G43600.1 (ureidoglycolate amidohydrolase )

HSP 1 Score: 42.7 bits (99), Expect = 8.8e-04
Identity = 32/115 (27.83%), Postives = 43/115 (37.39%), Query Frame = 0


Query: 86  KWPGSNPELPSILLNSHTDVVPAEHKKWMHPPLGAHIDSHGNIYARGSQDMKCVGMQYLE 145
           KW G  P LP++   SH D +P                        G  D     +  +E
Sbjct: 122 KWDGLEPNLPAVATGSHIDAIPYS----------------------GKYDGVVGVLGAIE 181

Query: 146 AIRRLKASGFQPLRSVYLSFVPDEEIGGHG----GAEKFAESDEFKKLNVAIVLD 197
           AI  LK SGF+P RS+ +     EE    G    G+   A S E  +     V+D
Sbjct: 182 AINVLKRSGFKPKRSLEIILFTSEEPTRFGISCLGSRLLAGSKELAEALKTTVVD 214

The following BLAST results are available for this feature:

BLAST of CSPI06G09570 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99JW2	8.9e-102	45.01	Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1	[more]
Q6AYS7	2.4e-99	44.01	Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1	[more]
Q55DP8	7.1e-99	45.02	Aminoacylase-1 OS=Dictyostelium discoideum OX=44689 GN=acy1 PE=2 SV=1	[more]
Q6PTT0	2.1e-98	42.62	Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1	[more]
Q03154	6.6e-97	42.37	Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1	[more]

BLAST of CSPI06G09570 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KAV8	1.8e-259	99.11	N-acyl-L-amino-acid amidohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G117740 PE...	[more]
A0A5A7TXA6	2.3e-257	97.99	N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A5D3BSI0	6.6e-257	97.99	N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BJ13	6.6e-257	97.99	N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo OX=3656 GN=LOC103490443 PE=3 ...	[more]
A0A6J1CII7	4.7e-231	87.28	N-acyl-L-amino-acid amidohydrolase OS=Momordica charantia OX=3673 GN=LOC11101127...	[more]

BLAST of CSPI06G09570 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139978.1	3.8e-259	99.11	aminoacylase-1 [Cucumis sativus] >KGN46643.1 hypothetical protein Csa_005514 [Cu...	[more]
KAA0047810.1	4.7e-257	97.99	aminoacylase-1 [Cucumis melo var. makuwa]	[more]
XP_008448158.1	1.4e-256	97.99	PREDICTED: aminoacylase-1 [Cucumis melo] >TYK01049.1 aminoacylase-1 [Cucumis mel...	[more]
XP_038876663.1	8.8e-248	93.75	aminoacylase-1 [Benincasa hispida]	[more]
XP_023532856.1	4.0e-232	88.42	aminoacylase-1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI06G09570 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38220.1	4.6e-186	73.13	Peptidase M20/M25/M40 family protein	[more]
AT4G38220.2	1.9e-184	72.62	Peptidase M20/M25/M40 family protein	[more]
AT1G44820.1	1.2e-149	54.34	Peptidase M20/M25/M40 family protein	[more]
AT1G44180.1	1.9e-147	54.76	Peptidase M20/M25/M40 family protein	[more]
AT5G43600.1	8.8e-04	27.83	ureidoglycolate amidohydrolase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.630.10	Zn peptidases	coord: 27..216 e-value: 2.5E-59 score: 202.1
None	No IPR available	GENE3D	1.10.150.900		coord: 354..434 e-value: 1.7E-30 score: 106.6
None	No IPR available	PIRSF	PIRSF036696	ACY-1	coord: 23..437 e-value: 3.4E-176 score: 583.6
None	No IPR available	GENE3D	3.30.70.360		coord: 217..327 e-value: 6.0E-8 score: 34.5
None	No IPR available	PANTHER	PTHR45892:SF4	BNAA06G40880D PROTEIN	coord: 19..438
None	No IPR available	PANTHER	PTHR45892	AMINOACYLASE-1	coord: 19..438
None	No IPR available	CDD	cd05646	M20_AcylaseI_like	coord: 35..433 e-value: 0.0 score: 662.431
None	No IPR available	SUPERFAMILY	53187	Zn-dependent exopeptidases	coord: 32..433
IPR010159	N-acyl-L-amino-acid amidohydrolase	TIGRFAM	TIGR01880	TIGR01880	coord: 32..434 e-value: 3.0E-187 score: 620.2
IPR002933	Peptidase M20	PFAM	PF01546	Peptidase_M20	coord: 98..430 e-value: 1.0E-36 score: 126.6
IPR001261	ArgE/DapE/ACY1/CPG2/YscS, conserved site	PROSITE	PS00758	ARGE_DAPE_CPG2_1	coord: 97..106
IPR001261	ArgE/DapE/ACY1/CPG2/YscS, conserved site	PROSITE	PS00759	ARGE_DAPE_CPG2_2	coord: 133..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G09570.1	CSPI06G09570.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006520	cellular amino acid metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004046	aminoacylase activity
molecular_function	GO:0016787	hydrolase activity