CSPI06G00780 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G00780
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Diacylglycerol kinase family protein
Location	Chr6: 556550 .. 561662 (-)
RNA-Seq Expression	CSPI06G00780
Synteny	CSPI06G00780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAACACAAAAGTTAGTACCAAACAGATTATCGAAATCTACAGTTTTCTCCTTCAAAATAATCGGAATCAAACGGCACCCAGCAAAGAGATTCTGATTTACGGAGATCAAATGGACATGAATTGCCGCAAATCTTGAAAAAGACCGTGAAATTTTGAATCTTTTCTTCAAGCAACTGCGTTAATGTCAATGTGAAGTTTTAATAATCATCACCTTAATGTCATTATCCATTTCCTTCTTTTACGATTATTATTATTATTTCCCCAAATCGTACTCTATAATTTCCTAACCTATTGGACAAGGAAATTTCAGCGATTAAGAAGATGGTTGTTGTTTCCCTTTGCTATCACTTAGCATTGGGACCTTCCATGGCGACAAAACCCTTTATCACCATGGCCGAACAGCCCATTGCGCCCGATCTCTATGTCGATCGGTCAACTTTTCATCGTGGGTCGTCTTCTTTCTCTTCATCTTCTGGCCGTCGCGATCTTGTTTTTGTTGTTAATCCCCGCGGTTCGTTTCTCGTCACTTAATTTTCTGTTTTTTTTATTGGAAGAGGAATTTAGTTGAAAAATGGGAAATTTAGAATGTGGGTTTTTTGTATTTGTGTCGTAATTGAAGATTTCAATGCTTTGAGTTTATTGGAACTTCATTGGAGATTGTAATTTTTCTGGGGTTTTCCAGTTCTTATGGTATTGGCCATTGGGTGATTTCTAGTCATGGAGTTTGATCATGTTTTAGCCCTTATGCAGGTGCAAATGGTAAGACGGCGAAGCAATGGAAGAAGCTCGTTCCATATCTACGCACTCGGCTTGGTGAACATTATAATGTGAGAATTTTTTCTCTTGTTATCTGTGGGGAATTGTGCGTATGAGTCTAATTGTGTTCCTAGGCTCAACTTCGCTATCTCCATTGAAGTAAATCTAGCATATCGAGTTATATTTATGGAGCTTATTGGAGTAACTGATTTTGCAAAAGAAATACTAGATTCAAAGACTTTGGGTGGAGCTATTTTCTGACATAGTTTACATGTTTGGAATTTGAATATCTGTTTTTTAGGAGTTGTTTTCCATATTTTTCTTTCTATTTGTTCGCCTGTTTATTAAAATAATTGAACTTGCTTGTTAAACCTCCAATTGTGAATTTTTGTGCCAGAAGGGACAAAAGAAGTATTTTTGTCACTAGCAGCTAGGACAGTTTTTATTATTAGGATTGTTGGTAGTTATTAGAATTTGGTGAGTTAGGAAGTTATAACTTCCTTTGTTGGTTATGATTGTGCCCTTCTTGAATTGATCATACCTGATAGGTTATATGATCTCTCTAGAGAATTTTAAGTAAATAAGGATACCTATAATTATTCTCCCTTCAAACATCATGACAACTAGCCTTGGCATTGACGAAAGGAGATTTTTAAAAAGGATCACAAATATGTGCTTGCTTGAGCTTAGAGTGTAAAATCTAGTTTTATTTTTGATCAAGTATTCCGTTCTTTACGTACAACTCCCACAAAGATTGTCCAACGACCCACCATTTTAATTGAATAACCATGCGGTTCTAATTAAACCCGCCAGTGTACTATTGCATTAATCTAAGTGGGTTGCATCTTCAATGACAGAGGAAAACCTTTTTGACAAATCTGTGGATAATATATCCTTTTAGGTTCTCTCTTGGTGTACAATTTTTTCTTATTTGCATGACTACACTGAGCACTCTTTTAGCCAATTGGTCATTTTGTAGCATCCTAAGGCTACGGTTTGCTACTTCCTTGTATATTCTATCTCATCGATGAAATGTTAGAATATTTCTTATTTTGAGAAAATAATAAATATATAATAAGAAGAAAGTTCCTTATCTTATCGTCAGCATATTTGTATCTCATTTGTACATTTTTCATAGATCTGACCTTGTTCCTTTAAAGGATCTATTATTTGCTTTTATTAAAATTTCAAATTGGCTAATGATCCATATCTTCATTCATTCTTTATGTTATTTTGATCTTTTTTTCCAAATATCGTTGAGCCCAGAAAATCGTAATAGTTCCTTATCATATTATGATGGAGTATATAAGTGAATAGTTCCGTGTGCACTAGTTTGTGTAGCTTGAAATTTATCTATGAACTAACTAAACAGTTCACTTTTGTGGACAGATATGTGAATCTCTGACGTCAGGTCCTTGCCATGCCATTGATATAACGAGGGAGGTCTGTCTGAATTAGTTTTTTGTGGAAGTTCAGATAGTTACTATCGATCTTTTTGTTGACTTAATGACTTATAGGCAATTCGGGAAGGTGCTGATGCTGTGATTGCTGTAGGAGGAGATGGAACTTTTCATGAGGTATGATACGACTCATTTCTATTAGTTCAGTTTTCAATGCTTAGCGCAGACTTGGTTTTACCTTTTCTTTTTAGTCATCTTTGGTTTAAGCCAGTAAACCAAGCCAAACTGCTTGCACGCCCAAGTTGAATGATGTGATTGCTCGATTGGTATATGAACATCATTTCTTTTTGTTTCTCTTGAACACCTCCAAACTTTTTGAAGCTATTTTTTGTATCTATCCATAGTTTTTGAGCCCGGAGATTTCAGTGGTAACATCTAACCATTAGTATAGCTTGAGTATGTCTTCTTCTTCTCCTACTAACTTAGTACTTGATAATGATTAATTACCAAGGTCTGTTTTCTATAAAAGTAACTTTTAATGGCTTTGATTCAAGCAACCCTCTTGTATTGCCCATTAGATATAAGCAAGTATTTTACAACTTTTAATGAAATTTTCTAGGATTGATGTTTGGTTGTATACGTTTGACTTGATGCATGAGGGGATTAATCTGTTCTGATTGACAGGTGGTTAATGGCTTCTTTTGGGCTGGAAAGCCTATTGTTAATAATGATGGAGAAGCACGTAAATCAACAGCACTTGGTGTAAGAGATTCCTTCATTGTTACTATCTTAAGTTGCTTATTTCCATTATCTACTAGCTGCTGGTTTATCAGTCTGTAACTTGTTCAGATTATTTTCATGAATTTATGCTATTGAATTGTTGGATTACATATATTATTGTGCCTACTTGCAGATTATTCCCTTGGGGACAGGTTCGGATTTTGCAAGAACATTTGGTTGGTAAGTGCACATTACTGCATTGTGTTTTTGTACGATGTGAGACTTTCAATTATCACTTCCTCCATGGGGTTAAGCAAACTTGGTTAACATTCTTTTAATTAGGAATAACAATCCTTTTGAAGCTGTGGACCATATAGCCAAAGGTATCCTTGTTTCACTCTTTTGTCTTCCCCTCTAATTTTTCTGTCGATGAAGTACTGTAAATGGCTCTGTTTGTTCACACAAACATTGCATTACCATTGGTAGTTCATGCAAGATATTGTGCAGGGTTGAGGACCCGAATTGATGTTGGAGTTGTCAATGAAGATAATGGGGAGAATCATTACTTTGCAAATGTTGCAGACATTCATTTGTAAGGTTAATCTAAACAGTATCATTGATGCTAACCGACAAACTATGCTGATTAAGGTCATCTAATTATCTTATTGCATGGATCTTTTTGCAGGAGTGCAAAAGCTGGTTAAGAGTTTAGCTACCCCATACTTCACAATCTAAAACACAAAACTCAATATAATACCCATAACAAAAATTCAAGGAGAAGAAAAAGAAAAAGAAGAAGAAAAGCTAGTGATTCGTATTATTATCAGACTGTGTATAACAGTATGTGTTTTTTAATATTCTTTGTTTCCAGCTCCAGTGTACCAAAATATTATCACAAACTCCACAATCCTTCTCTTTCCAATTCCTAAACTAAAAGATAATAGATTTAAACAATAAATCAAGTTATTCGAGGTACTTGGATCCTCCCTTCTTGAGATCACAATCTCAAGCCCTAAAACCCAAATATTGCTTCTCTTCTCCATTCCCCTATCCCTCTATTTATAACCAAATGTAACCCACTCCCTAACTATTTATGCCCTCAATACCCAAATAACCCCTAATACCGATCCTATTCGTCTCAAGGTGGTTTCAAAATGTAGACCATTTTGGAGGGGGCGGTGACTGCATTTTTACCATCTTTATTTAATTTCATCTATGTAGAATGCCATAACTTACAGGTTGATGATGGTGAATGGGAGCTGTATCCTCAAGTAACTGCTCTATGCATTGGAAATGCTAAATACTTTGGCGGTGGTATGAAAATTGTGCCAAATGCCGATCCTTCCAACGGGAGTCTAGAGGTATAATTTGTTTCTGTGGAATTTTATTTGATCACTTTTTCCTTCCTTATCTCCTTAACTATGTGATGCTTTCCCTCTGCTTTCTTTGCAGGTAGTAATTCTTCAGGATTTCAAGTGGTATGACTTTATTCTGAATTTGCATAAAATATACAATGGGACACATTTGACGGTGAAAAACGTAACATCAAGAAGGTTAGTCATCTCTTTAAAAATTATAATGTGCCTAGATTTTCCATTTTGTATTTCAATTTTCTCTCTCCAACGTCTGTTTTATGCTGGAGACTTGTGGAAGTAGAGGTGAAGAAACTATAATTGAACTGGATTTGCATGGAGAATTGTGATTTTCATTTCTGTCAAGTTTTTGCTTGAGAAAAACTTACACTCAAACTTATATGGAGATGCCTTATTTACCTAGCAGAGAAGCCATAAGAAAAGTAATATTGTTAAACCTTCTGACTTCTGCCATTCTGATGTATCCTCAACCAAAATTAGAAAGCTCATATGATTATCCTTTTTTCCTTGTTAAGTCTTTGTTAACGTTGATGAACTCAATTCAAAATTTTTGATCGATATCAATGTAACTCTTCCATGGTTGAACATATGATTGAGCATGATTTCACATTTCAGCTCCATCATTGTTTGGCAATGATGTCCTTTTCTCAATCAAAACTTCCTATTACTGCTTCTGGGTTTGTTCGTTTGCAATGCAACTAATCATCAGTTTATCGTTTTGTTGTTCAAGCAGCGTTCGTTCAATCGAAGTGGAAGAAGTTTCTTGCAGCGGCAGCATATATGTTCAATCTGATGGGGAACACTTGGGGTTTCTTCCAAGGAAATTCCATATTCTACCAGCTGCCATTGAAATGATATGCTGA

mRNA sequence

GAAACACAAAAGTTAGTACCAAACAGATTATCGAAATCTACAGTTTTCTCCTTCAAAATAATCGGAATCAAACGGCACCCAGCAAAGAGATTCTGATTTACGGAGATCAAATGGACATGAATTGCCGCAAATCTTGAAAAAGACCGTGAAATTTTGAATCTTTTCTTCAAGCAACTGCGTTAATGTCAATGTGAAGTTTTAATAATCATCACCTTAATGTCATTATCCATTTCCTTCTTTTACGATTATTATTATTATTTCCCCAAATCGTACTCTATAATTTCCTAACCTATTGGACAAGGAAATTTCAGCGATTAAGAAGATGGTTGTTGTTTCCCTTTGCTATCACTTAGCATTGGGACCTTCCATGGCGACAAAACCCTTTATCACCATGGCCGAACAGCCCATTGCGCCCGATCTCTATGTCGATCGGTCAACTTTTCATCGTGGGTCGTCTTCTTTCTCTTCATCTTCTGGCCGTCGCGATCTTGTTTTTGTTGTTAATCCCCGCGGTGCAAATGGTAAGACGGCGAAGCAATGGAAGAAGCTCGTTCCATATCTACGCACTCGGCTTGGTGAACATTATAATATATGTGAATCTCTGACGTCAGGTCCTTGCCATGCCATTGATATAACGAGGGAGGCAATTCGGGAAGGTGCTGATGCTGTGATTGCTGTAGGAGGAGATGGAACTTTTCATGAGGTGGTTAATGGCTTCTTTTGGGCTGGAAAGCCTATTGTTAATAATGATGGAGAAGCACGTAAATCAACAGCACTTGGTATTATTCCCTTGGGGACAGGTTCGGATTTTGCAAGAACATTTGGTTGGAATAACAATCCTTTTGAAGCTGTGGACCATATAGCCAAAGGGTTGAGGACCCGAATTGATGTTGGAGTTGTCAATGAAGATAATGGGGAGAATCATTACTTTGCAAATGTTGCAGACATTCATTTGAGTGCAAAAGCTGAATGCCATAACTTACAGGTTGATGATGGTGAATGGGAGCTGTATCCTCAAGTAACTGCTCTATGCATTGGAAATGCTAAATACTTTGGCGGTGGTATGAAAATTGTGCCAAATGCCGATCCTTCCAACGGGAGTCTAGAGGTAGTAATTCTTCAGGATTTCAAGTGGTATGACTTTATTCTGAATTTGCATAAAATATACAATGGGACACATTTGACGGTGAAAAACGTAACATCAAGAAGCGTTCGTTCAATCGAAGTGGAAGAAGTTTCTTGCAGCGGCAGCATATATGTTCAATCTGATGGGGAACACTTGGGGTTTCTTCCAAGGAAATTCCATATTCTACCAGCTGCCATTGAAATGATATGCTGA

Coding sequence (CDS)

ATGGTTGTTGTTTCCCTTTGCTATCACTTAGCATTGGGACCTTCCATGGCGACAAAACCCTTTATCACCATGGCCGAACAGCCCATTGCGCCCGATCTCTATGTCGATCGGTCAACTTTTCATCGTGGGTCGTCTTCTTTCTCTTCATCTTCTGGCCGTCGCGATCTTGTTTTTGTTGTTAATCCCCGCGGTGCAAATGGTAAGACGGCGAAGCAATGGAAGAAGCTCGTTCCATATCTACGCACTCGGCTTGGTGAACATTATAATATATGTGAATCTCTGACGTCAGGTCCTTGCCATGCCATTGATATAACGAGGGAGGCAATTCGGGAAGGTGCTGATGCTGTGATTGCTGTAGGAGGAGATGGAACTTTTCATGAGGTGGTTAATGGCTTCTTTTGGGCTGGAAAGCCTATTGTTAATAATGATGGAGAAGCACGTAAATCAACAGCACTTGGTATTATTCCCTTGGGGACAGGTTCGGATTTTGCAAGAACATTTGGTTGGAATAACAATCCTTTTGAAGCTGTGGACCATATAGCCAAAGGGTTGAGGACCCGAATTGATGTTGGAGTTGTCAATGAAGATAATGGGGAGAATCATTACTTTGCAAATGTTGCAGACATTCATTTGAGTGCAAAAGCTGAATGCCATAACTTACAGGTTGATGATGGTGAATGGGAGCTGTATCCTCAAGTAACTGCTCTATGCATTGGAAATGCTAAATACTTTGGCGGTGGTATGAAAATTGTGCCAAATGCCGATCCTTCCAACGGGAGTCTAGAGGTAGTAATTCTTCAGGATTTCAAGTGGTATGACTTTATTCTGAATTTGCATAAAATATACAATGGGACACATTTGACGGTGAAAAACGTAACATCAAGAAGCGTTCGTTCAATCGAAGTGGAAGAAGTTTCTTGCAGCGGCAGCATATATGTTCAATCTGATGGGGAACACTTGGGGTTTCTTCCAAGGAAATTCCATATTCTACCAGCTGCCATTGAAATGATATGCTGA

Protein sequence

MVVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVNPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHIAKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAECHNLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMIC*

Homology

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BLAST of CSPI06G00780 vs. ExPASy Swiss-Prot
Match: O82359 (Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LCKB2 PE=2 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 5.9e-130
Identity = 228/354 (64.41%), Postives = 274/354 (77.40%), Query Frame = 0


Query: 12  LGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVNPRGANGKTAK 71
           L P  A +P     +Q I  D +  R      +++  SSSG RDLVFVVNP+GANG+TAK
Sbjct: 10  LCPFTAKQPSFLRKQQRITSDRFTFRGGGGGDAAAVVSSSGLRDLVFVVNPQGANGRTAK 69

Query: 72  QWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNG 131
           +WKKL+P+LR+RLG+  N+ E LTSGP HAIDITREAIR+GADAVIAVGGDGT HEVVNG
Sbjct: 70  EWKKLLPHLRSRLGKDCNVSELLTSGPSHAIDITREAIRDGADAVIAVGGDGTLHEVVNG 129

Query: 132 FFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHIAKGLRTRIDVG 191
           FFW GKP+    GEA +STALG+IPLGTGSDFARTFGWNN+P EAV+ IA+G+R+RIDVG
Sbjct: 130 FFWEGKPVGYLSGEASRSTALGLIPLGTGSDFARTFGWNNDPCEAVERIARGMRSRIDVG 189

Query: 192 VVNEDNGENHYFANVADIHLSAKAE------------C------------HN----LQVD 251
           V++++  + HYF NVAD+HLSAKA             C            HN    ++V+
Sbjct: 190 VIDKEGKDLHYFINVADVHLSAKAGFYASKYKKFGNLCYVIGALQAFMGHHNQDMRIRVN 249

Query: 252 DGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYDFILNLHKIYN 311
            GEWE+YPQVTALC+GNAKYFGGGMKI PNA P NG+LEVV+LQ+FKWYDF+L LHK+YN
Sbjct: 250 GGEWEIYPQVTALCVGNAKYFGGGMKITPNAIPGNGNLEVVVLQNFKWYDFVLKLHKLYN 309

Query: 312 GTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 338
           GTHL+V NVTSRSV+SIEVEE++ SGSIYVQSDGEHLGFLPRKF +LP AI++I
Sbjct: 310 GTHLSVNNVTSRSVQSIEVEEITDSGSIYVQSDGEHLGFLPRKFQVLPGAIDII 363

[Close]

BLAST of CSPI06G00780 vs. ExPASy Swiss-Prot
Match: P9WP28 (Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=dagK PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.5e-24
Identity = 86/310 (27.74%), Postives = 136/310 (43.87%), Query Frame = 0


Query: 59  VVNPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIA 118
           + NP   +G   K     +  L+ R     ++ E +      A  +   A+ +G DAV+ 
Sbjct: 17  LTNPLSGHGAAVKAAHGAIARLKHR---GVDVVEIVGGDAHDARHLLAAAVAKGTDAVMV 76

Query: 119 VGGDGTFHEVVNGFFWAGKPIVNNDGEARKST--ALGIIPLGTGSDFARTFGW-NNNPFE 178
            GGDG               +V+N  +    T   LGIIP GTG+D AR FG    NP  
Sbjct: 77  TGGDG---------------VVSNALQVLAGTDIPLGIIPAGTGNDHAREFGLPTKNPKA 136

Query: 179 AVDHIAKGLRTRIDVGVVNEDNGENHYFANVA----------------------DIHLSA 238
           A D +  G    ID+G + +DNG   +F  VA                        +++ 
Sbjct: 137 AADIVVDGWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDRANRMRWPHGRMRYYIAM 196

Query: 239 KAECHNLQ------VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQ 298
            AE   L+      V DG  E+   +T    GN + +GGG+ I PNAD S+G L++ + Q
Sbjct: 197 LAELSRLRPLPFRLVLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMAQ 256

Query: 299 DFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKF 338
                  +     I+ G H+ +  V++   +++ VE   C G I V +DG+    LP + 
Sbjct: 257 SDSRTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVE---CPG-INVYADGDFACPLPAEI 304

[Close]

BLAST of CSPI06G00780 vs. ExPASy Swiss-Prot
Match: P9WP29 (Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=dagK PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.5e-24
Identity = 86/310 (27.74%), Postives = 136/310 (43.87%), Query Frame = 0


Query: 59  VVNPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIA 118
           + NP   +G   K     +  L+ R     ++ E +      A  +   A+ +G DAV+ 
Sbjct: 17  LTNPLSGHGAAVKAAHGAIARLKHR---GVDVVEIVGGDAHDARHLLAAAVAKGTDAVMV 76

Query: 119 VGGDGTFHEVVNGFFWAGKPIVNNDGEARKST--ALGIIPLGTGSDFARTFGW-NNNPFE 178
            GGDG               +V+N  +    T   LGIIP GTG+D AR FG    NP  
Sbjct: 77  TGGDG---------------VVSNALQVLAGTDIPLGIIPAGTGNDHAREFGLPTKNPKA 136

Query: 179 AVDHIAKGLRTRIDVGVVNEDNGENHYFANVA----------------------DIHLSA 238
           A D +  G    ID+G + +DNG   +F  VA                        +++ 
Sbjct: 137 AADIVVDGWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDRANRMRWPHGRMRYYIAM 196

Query: 239 KAECHNLQ------VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQ 298
            AE   L+      V DG  E+   +T    GN + +GGG+ I PNAD S+G L++ + Q
Sbjct: 197 LAELSRLRPLPFRLVLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMAQ 256

Query: 299 DFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKF 338
                  +     I+ G H+ +  V++   +++ VE   C G I V +DG+    LP + 
Sbjct: 257 SDSRTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVE---CPG-INVYADGDFACPLPAEI 304

[Close]

BLAST of CSPI06G00780 vs. ExPASy Swiss-Prot
Match: Q49YU2 (Diacylglycerol kinase OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) OX=342451 GN=dagK PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 3.1e-14
Identity = 75/265 (28.30%), Postives = 112/265 (42.26%), Query Frame = 0


Query: 103 DITREAIR---EGADAVIAVGGDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGT 162
           D T EA R   +  D +IA GGDGT +EV+NG   A KP            +LGIIP+GT
Sbjct: 46  DATTEAARSLEQNYDVLIAAGGDGTLNEVINGI--AEKP---------NRPSLGIIPMGT 105

Query: 163 GSDFARTFGWNNNPFEAVDHIAKGLRTRIDVGVVNEDNGENHYFANVA----------DI 222
            +DF R      +   A+D I +G  TR+D+G +N     + YF N+A          + 
Sbjct: 106 VNDFGRALHLPTDIMSAIDVIIEGHMTRVDIGKMN-----SRYFINLAAGGQLTQVSYET 165

Query: 223 HLSAKAECHNLQVDDGEWELYPQVTALCI----------GNAKYF--------GGGMKIV 282
               K+           +E+ PQ+ A+ I          G A  F         G  K+V
Sbjct: 166 PSKLKSIVGPFAYYIKGFEMLPQMKAVDIRIEYDDEVFQGEALLFLLGLTNSMAGFEKLV 225

Query: 283 PNADPSNGSLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSI 337
           P+A   +G   ++I++     +    +     G H     V  +  +SI V   +    +
Sbjct: 226 PDAKLDDGYFTLIIVEKANLAELGHIMTLASRGEHTKHPKVHYKKAKSISVSSFT---DM 285

[Close]

BLAST of CSPI06G00780 vs. ExPASy Swiss-Prot
Match: Q2YU29 (Diacylglycerol kinase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) OX=273036 GN=dagK PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.5e-13
Identity = 67/257 (26.07%), Postives = 107/257 (41.63%), Query Frame = 0


Query: 108 AIREGADAVIAVGGDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTF 167
           A+ E  D +IA GGDGT +EVVNG   A KP             LG+IP+GT +DF R  
Sbjct: 54  AMHENYDVLIAAGGDGTLNEVVNGI--AEKP---------NRPKLGVIPMGTVNDFGRAL 113

Query: 168 GWNNNPFEAVDHIAKGLRTRIDVGVVNEDNGENHYFANVA----------DIHLSAKAEC 227
              N+   A+D I +G  T++D+G +N     N YF N+A          +     K+  
Sbjct: 114 HIPNDIMGALDVIIEGHSTKVDIGKMN-----NRYFINLAAGGQLTQVSYETPSKLKSIV 173

Query: 228 HNLQVDDGEWELYPQVTAL------------------CIGNAKYFGGGMKIVPNADPSNG 287
                    +E+ PQ+ A+                   +G      G  K+VP+A   +G
Sbjct: 174 GPFAYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDG 233

Query: 288 SLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEH 337
              ++I++     +    +     G H     V     ++I +  ++    + +  DGE+
Sbjct: 234 YFTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSLT---DLQLNVDGEY 291

[Close]

BLAST of CSPI06G00780 vs. ExPASy TrEMBL
Match: A0A5D3BVA2 (Sphingoid long-chain bases kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001350 PE=4 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.1e-187
Identity = 327/366 (89.34%), Postives = 333/366 (90.98%), Query Frame = 0


Query: 1   MVVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVV 60
           MVVVSLCYHLALG SMATKPFITMAEQP APDLYVDRSTFHRGSSSFSSSSGRRDLVFVV
Sbjct: 1   MVVVSLCYHLALGSSMATKPFITMAEQPTAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVV 60

Query: 61  NPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG 120
           NPRGANGKTAK+WKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG
Sbjct: 61  NPRGANGKTAKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG 120

Query: 121 GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHI 180
           GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVD I
Sbjct: 121 GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRI 180

Query: 181 AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAE------------CH---------- 240
           AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKA             C+          
Sbjct: 181 AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMG 240

Query: 241 ------NLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY 300
                  ++VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY
Sbjct: 241 HQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY 300

Query: 301 DFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA 339
           DFILNLHKIYNGTHLTVKNVTS+S+RSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA
Sbjct: 301 DFILNLHKIYNGTHLTVKNVTSKSIRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA 360

[Close]

BLAST of CSPI06G00780 vs. ExPASy TrEMBL
Match: A0A1S3C7U6 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103497689 PE=4 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.1e-187
Identity = 327/366 (89.34%), Postives = 333/366 (90.98%), Query Frame = 0


Query: 1   MVVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVV 60
           MVVVSLCYHLALG SMATKPFITMAEQP APDLYVDRSTFHRGSSSFSSSSGRRDLVFVV
Sbjct: 1   MVVVSLCYHLALGSSMATKPFITMAEQPTAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVV 60

Query: 61  NPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG 120
           NPRGANGKTAK+WKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG
Sbjct: 61  NPRGANGKTAKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG 120

Query: 121 GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHI 180
           GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVD I
Sbjct: 121 GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRI 180

Query: 181 AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAE------------CH---------- 240
           AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKA             C+          
Sbjct: 181 AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMG 240

Query: 241 ------NLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY 300
                  ++VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY
Sbjct: 241 HQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY 300

Query: 301 DFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA 339
           DFILNLHKIYNGTHLTVKNVTS+S+RSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA
Sbjct: 301 DFILNLHKIYNGTHLTVKNVTSKSIRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA 360

[Close]

BLAST of CSPI06G00780 vs. ExPASy TrEMBL
Match: A0A6J1KFE4 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111494546 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.4e-171
Identity = 298/365 (81.64%), Postives = 316/365 (86.58%), Query Frame = 0


Query: 2   VVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61
           +V   CYHLA  PSMATKPF+T AEQPIAPDL  DRS FHRGSSSF SSSGRRDLVFVVN
Sbjct: 1   MVECFCYHLAFRPSMATKPFVTRAEQPIAPDLNADRSIFHRGSSSF-SSSGRRDLVFVVN 60

Query: 62  PRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121
           PRGANGKT K+WKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVI VGG
Sbjct: 61  PRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIPVGG 120

Query: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHIA 181
           DGTFHEVVNGFFW GKP+VNNDGE  KSTALG+IPLGTGSDFARTFGWNNNP EAVD IA
Sbjct: 121 DGTFHEVVNGFFWDGKPVVNNDGEVHKSTALGLIPLGTGSDFARTFGWNNNPIEAVDRIA 180

Query: 182 KGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAE------------CH----------- 241
           KGLR+RID+GVVN++NGE HYFANVADIHLSAKA             C+           
Sbjct: 181 KGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGFIGH 240

Query: 242 -----NLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYD 301
                 ++VD+GEWELYPQVT+LCIGNAKYFGGGMKIVPNADPSNG+LE+VILQDFKWYD
Sbjct: 241 KNKDLKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLELVILQDFKWYD 300

Query: 302 FILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAA 339
           FILNLHKIYNGTHLTVKNV+SRSVRSIEVEEVS SGSIYVQ+DGEHLGFLPRKFHILPAA
Sbjct: 301 FILNLHKIYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQADGEHLGFLPRKFHILPAA 360

[Close]

BLAST of CSPI06G00780 vs. ExPASy TrEMBL
Match: A0A6J1HNB5 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464490 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.5e-170
Identity = 297/365 (81.37%), Postives = 315/365 (86.30%), Query Frame = 0


Query: 2   VVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61
           +V   CYHLA  P MA+KPF+T AEQPIAPDL  DRS FHRGSSSF SSSGRRDLVFVVN
Sbjct: 1   MVECFCYHLAFRPFMASKPFVTRAEQPIAPDLNADRSVFHRGSSSF-SSSGRRDLVFVVN 60

Query: 62  PRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121
           PRGANGKT K+WKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVI VGG
Sbjct: 61  PRGANGKTLKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIPVGG 120

Query: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHIA 181
           DGTFHEVVNGFFW GKP+VNNDGE  KSTALG+IPLGTGSDFARTFGWNNNP EAVD IA
Sbjct: 121 DGTFHEVVNGFFWDGKPVVNNDGEVHKSTALGLIPLGTGSDFARTFGWNNNPIEAVDRIA 180

Query: 182 KGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAE------------CH----------- 241
           KGLR+RID+GVVN++NGE HYFANVADIHLSAKA             C+           
Sbjct: 181 KGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGFIGH 240

Query: 242 -----NLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYD 301
                 ++VD+GEWELYPQVT+LCIGNAKYFGGGMKIVPNADPSNG+LEVVILQDFKWYD
Sbjct: 241 KNKDFKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFKWYD 300

Query: 302 FILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAA 339
           FILNLHKIYNGTHLTVKNV+SRSVRSIEVEEVS SGSIYVQ+DGEHLGFLPRKFHILPAA
Sbjct: 301 FILNLHKIYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQADGEHLGFLPRKFHILPAA 360

[Close]

BLAST of CSPI06G00780 vs. ExPASy TrEMBL
Match: A0A6J1CZJ4 (sphingoid long-chain bases kinase 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016221 PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.3e-169
Identity = 291/365 (79.73%), Postives = 312/365 (85.48%), Query Frame = 0


Query: 2   VVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61
           +VV  CYH+ALG SMATKPF+T AEQPIAPDLY DRS FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 1   MVVCFCYHIALGASMATKPFVTRAEQPIAPDLYADRSVFHRGSSSFSSSSGRRDLVFVVN 60

Query: 62  PRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121
           PRGANGKT K+W KLVPYLRTRL EHYNICESLTSGPCHAIDITREAIREGA+AVIAVGG
Sbjct: 61  PRGANGKTVKEWNKLVPYLRTRLSEHYNICESLTSGPCHAIDITREAIREGAEAVIAVGG 120

Query: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHIA 181
           DGTFHEVVNGFFWAGKP++NNDG ARK TALG+IPLGTGSDFAR FGW NNPFEAVD IA
Sbjct: 121 DGTFHEVVNGFFWAGKPVINNDGAARKPTALGLIPLGTGSDFARAFGWKNNPFEAVDRIA 180

Query: 182 KGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAE------------CH----------- 241
           KGLR++IDVGV +E+NGE HYF NVADIHLSAKA             C+           
Sbjct: 181 KGLRSQIDVGVTHEENGEYHYFVNVADIHLSAKAGFYAARYKRFGNLCYVIGALQAFMGH 240

Query: 242 -----NLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYD 301
                 ++V+DGEWE+YPQVTALCIGNAKYFGGGMKI PNADP NG+LEVV+LQDFKWYD
Sbjct: 241 QNKDLKIKVNDGEWEVYPQVTALCIGNAKYFGGGMKITPNADPFNGNLEVVVLQDFKWYD 300

Query: 302 FILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAA 339
           FIL LHK+YNGTHLTVKNV+SR V SIEVEEVS SGSIYVQSDGEHLGFLPRKFHILPAA
Sbjct: 301 FILKLHKLYNGTHLTVKNVSSRRVHSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAA 360

[Close]

BLAST of CSPI06G00780 vs. NCBI nr
Match: XP_031743330.1 (sphingoid long-chain bases kinase 2, mitochondrial [Cucumis sativus] >KAE8646552.1 hypothetical protein Csa_005226 [Cucumis sativus])

HSP 1 Score: 673.3 bits (1736), Expect = 1.1e-189
Identity = 332/366 (90.71%), Postives = 335/366 (91.53%), Query Frame = 0


Query: 1   MVVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVV 60
           MVVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVV
Sbjct: 1   MVVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVV 60

Query: 61  NPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG 120
           NPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG
Sbjct: 61  NPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG 120

Query: 121 GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHI 180
           GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVD I
Sbjct: 121 GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRI 180

Query: 181 AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAE------------CH---------- 240
           AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKA             C+          
Sbjct: 181 AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMG 240

Query: 241 ------NLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY 300
                  ++VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY
Sbjct: 241 HQNRDFRIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY 300

Query: 301 DFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA 339
           DFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA
Sbjct: 301 DFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA 360

[Close]

BLAST of CSPI06G00780 vs. NCBI nr
Match: XP_008458171.1 (PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Cucumis melo] >TYK03040.1 sphingoid long-chain bases kinase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 665.6 bits (1716), Expect = 2.3e-187
Identity = 327/366 (89.34%), Postives = 333/366 (90.98%), Query Frame = 0


Query: 1   MVVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVV 60
           MVVVSLCYHLALG SMATKPFITMAEQP APDLYVDRSTFHRGSSSFSSSSGRRDLVFVV
Sbjct: 1   MVVVSLCYHLALGSSMATKPFITMAEQPTAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVV 60

Query: 61  NPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG 120
           NPRGANGKTAK+WKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG
Sbjct: 61  NPRGANGKTAKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVG 120

Query: 121 GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHI 180
           GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVD I
Sbjct: 121 GDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRI 180

Query: 181 AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAE------------CH---------- 240
           AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKA             C+          
Sbjct: 181 AKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMG 240

Query: 241 ------NLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY 300
                  ++VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY
Sbjct: 241 HQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWY 300

Query: 301 DFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA 339
           DFILNLHKIYNGTHLTVKNVTS+S+RSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA
Sbjct: 301 DFILNLHKIYNGTHLTVKNVTSKSIRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPA 360

[Close]

BLAST of CSPI06G00780 vs. NCBI nr
Match: XP_038876180.1 (sphingoid long-chain bases kinase 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 638.3 bits (1645), Expect = 3.9e-179
Identity = 314/365 (86.03%), Postives = 323/365 (88.49%), Query Frame = 0


Query: 2   VVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61
           +VVS CYHLALGPSMATKPFIT AEQP+APD Y DRS FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 1   MVVSFCYHLALGPSMATKPFITRAEQPMAPDFYADRSIFHRGSSSFSSSSGRRDLVFVVN 60

Query: 62  PRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121
           PRGANGKT K+WKKLVPYLRTRLGE YNICESLTSGPCHAIDITREAIREGADAVIAVGG
Sbjct: 61  PRGANGKTVKEWKKLVPYLRTRLGEQYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120

Query: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHIA 181
           DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVD IA
Sbjct: 121 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIA 180

Query: 182 KGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAE------------CH----------- 241
           KGLR+RIDVGVVNE+NG  HYFANVADIHLSAKA             C+           
Sbjct: 181 KGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYASRYKKFGNLCYVIGALQGFMGH 240

Query: 242 -----NLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYD 301
                 ++VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADP NG+LEVVILQDFKWYD
Sbjct: 241 QNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPCNGNLEVVILQDFKWYD 300

Query: 302 FILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAA 339
           FILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVS SGSIYVQSDGEHLGFLPRKFHILPAA
Sbjct: 301 FILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAA 360

[Close]

BLAST of CSPI06G00780 vs. NCBI nr
Match: XP_023514489.1 (sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 611.3 bits (1575), Expect = 5.0e-171
Identity = 299/365 (81.92%), Postives = 316/365 (86.58%), Query Frame = 0


Query: 2   VVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61
           +V   CYHLA  PSMATKPF+T AEQPIAPDL  DRS FHRGSSSF SSSGRRDLVFVVN
Sbjct: 1   MVECFCYHLAFRPSMATKPFVTRAEQPIAPDLNADRSIFHRGSSSF-SSSGRRDLVFVVN 60

Query: 62  PRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121
           PRGANGKT K+WKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVI VGG
Sbjct: 61  PRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIPVGG 120

Query: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHIA 181
           DGTFHEVVNGFFW GKP+VNNDGE  KSTALG+IPLGTGSDFARTFGWNNNP EAVD IA
Sbjct: 121 DGTFHEVVNGFFWDGKPVVNNDGEVHKSTALGLIPLGTGSDFARTFGWNNNPIEAVDRIA 180

Query: 182 KGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAE------------CH----------- 241
           KGLR+RID+GVVN++NGE HYFANVADIHLSAKA             C+           
Sbjct: 181 KGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGFIGH 240

Query: 242 -----NLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYD 301
                 ++VD+GEWELYPQVT+LCIGNAKYFGGGMKIVPNADPSNG+LEVVILQDFKWYD
Sbjct: 241 KNKDFKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFKWYD 300

Query: 302 FILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAA 339
           FILNLHKIYNGTHLTVKNV+SRSVRSIEVEEVS SGSIYVQ+DGEHLGFLPRKFHILPAA
Sbjct: 301 FILNLHKIYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQADGEHLGFLPRKFHILPAA 360

[Close]

BLAST of CSPI06G00780 vs. NCBI nr
Match: XP_023000266.1 (sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 298/365 (81.64%), Postives = 316/365 (86.58%), Query Frame = 0


Query: 2   VVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61
           +V   CYHLA  PSMATKPF+T AEQPIAPDL  DRS FHRGSSSF SSSGRRDLVFVVN
Sbjct: 1   MVECFCYHLAFRPSMATKPFVTRAEQPIAPDLNADRSIFHRGSSSF-SSSGRRDLVFVVN 60

Query: 62  PRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121
           PRGANGKT K+WKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVI VGG
Sbjct: 61  PRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIPVGG 120

Query: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHIA 181
           DGTFHEVVNGFFW GKP+VNNDGE  KSTALG+IPLGTGSDFARTFGWNNNP EAVD IA
Sbjct: 121 DGTFHEVVNGFFWDGKPVVNNDGEVHKSTALGLIPLGTGSDFARTFGWNNNPIEAVDRIA 180

Query: 182 KGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAE------------CH----------- 241
           KGLR+RID+GVVN++NGE HYFANVADIHLSAKA             C+           
Sbjct: 181 KGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGFIGH 240

Query: 242 -----NLQVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYD 301
                 ++VD+GEWELYPQVT+LCIGNAKYFGGGMKIVPNADPSNG+LE+VILQDFKWYD
Sbjct: 241 KNKDLKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLELVILQDFKWYD 300

Query: 302 FILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAA 339
           FILNLHKIYNGTHLTVKNV+SRSVRSIEVEEVS SGSIYVQ+DGEHLGFLPRKFHILPAA
Sbjct: 301 FILNLHKIYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQADGEHLGFLPRKFHILPAA 360

[Close]

BLAST of CSPI06G00780 vs. TAIR 10
Match: AT2G46090.1 (Diacylglycerol kinase family protein )

HSP 1 Score: 465.3 bits (1196), Expect = 4.2e-131
Identity = 228/354 (64.41%), Postives = 274/354 (77.40%), Query Frame = 0


Query: 12  LGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVNPRGANGKTAK 71
           L P  A +P     +Q I  D +  R      +++  SSSG RDLVFVVNP+GANG+TAK
Sbjct: 10  LCPFTAKQPSFLRKQQRITSDRFTFRGGGGGDAAAVVSSSGLRDLVFVVNPQGANGRTAK 69

Query: 72  QWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNG 131
           +WKKL+P+LR+RLG+  N+ E LTSGP HAIDITREAIR+GADAVIAVGGDGT HEVVNG
Sbjct: 70  EWKKLLPHLRSRLGKDCNVSELLTSGPSHAIDITREAIRDGADAVIAVGGDGTLHEVVNG 129

Query: 132 FFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDHIAKGLRTRIDVG 191
           FFW GKP+    GEA +STALG+IPLGTGSDFARTFGWNN+P EAV+ IA+G+R+RIDVG
Sbjct: 130 FFWEGKPVGYLSGEASRSTALGLIPLGTGSDFARTFGWNNDPCEAVERIARGMRSRIDVG 189

Query: 192 VVNEDNGENHYFANVADIHLSAKAE------------C------------HN----LQVD 251
           V++++  + HYF NVAD+HLSAKA             C            HN    ++V+
Sbjct: 190 VIDKEGKDLHYFINVADVHLSAKAGFYASKYKKFGNLCYVIGALQAFMGHHNQDMRIRVN 249

Query: 252 DGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYDFILNLHKIYN 311
            GEWE+YPQVTALC+GNAKYFGGGMKI PNA P NG+LEVV+LQ+FKWYDF+L LHK+YN
Sbjct: 250 GGEWEIYPQVTALCVGNAKYFGGGMKITPNAIPGNGNLEVVVLQNFKWYDFVLKLHKLYN 309

Query: 312 GTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 338
           GTHL+V NVTSRSV+SIEVEE++ SGSIYVQSDGEHLGFLPRKF +LP AI++I
Sbjct: 310 GTHLSVNNVTSRSVQSIEVEEITDSGSIYVQSDGEHLGFLPRKFQVLPGAIDII 363

[Close]

BLAST of CSPI06G00780 vs. TAIR 10
Match: AT5G23450.1 (long-chain base (LCB) kinase 1 )

HSP 1 Score: 58.5 bits (140), Expect = 1.2e-08
Identity = 37/138 (26.81%), Postives = 63/138 (45.65%), Query Frame = 0


Query: 56  LVFVVNPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADA 115
           ++ ++NPR  +G++ K +  +V  +    G    + ++  +G    +  T + I   +D 
Sbjct: 250 MLVILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVD-INLCSDG 309

Query: 116 VIAVGGDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFE 175
           +I VGGDG  +EV+NG      P      +   S  +GI+P G+ +    T     +P  
Sbjct: 310 IICVGGDGIINEVLNGLLTRSNP------KEGVSIPIGIVPAGSDNSLVWTVLGVRDPIS 369

Query: 176 AVDHIAKGLRTRIDVGVV 194
           A   I KG  T  DV  V
Sbjct: 370 AALSIVKGGLTATDVFAV 380

[Close]

BLAST of CSPI06G00780 vs. TAIR 10
Match: AT5G23450.2 (long-chain base (LCB) kinase 1 )

HSP 1 Score: 58.5 bits (140), Expect = 1.2e-08
Identity = 37/138 (26.81%), Postives = 63/138 (45.65%), Query Frame = 0


Query: 56  LVFVVNPRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADA 115
           ++ ++NPR  +G++ K +  +V  +    G    + ++  +G    +  T + I   +D 
Sbjct: 250 MLVILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVD-INLCSDG 309

Query: 116 VIAVGGDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFE 175
           +I VGGDG  +EV+NG      P      +   S  +GI+P G+ +    T     +P  
Sbjct: 310 IICVGGDGIINEVLNGLLTRSNP------KEGVSIPIGIVPAGSDNSLVWTVLGVRDPIS 369

Query: 176 AVDHIAKGLRTRIDVGVV 194
           A   I KG  T  DV  V
Sbjct: 370 AALSIVKGGLTATDVFAV 380

[Close]

BLAST of CSPI06G00780 vs. TAIR 10
Match: AT5G51290.1 (Diacylglycerol kinase family protein )

HSP 1 Score: 43.5 bits (101), Expect = 3.9e-04
Identity = 25/83 (30.12%), Postives = 44/83 (53.01%), Query Frame = 0


Query: 54  RDLVFVVNPRGANGKTAKQWKKLVP-YLRTRLGEHYNICESLTSGPCHAIDITREAIREG 113
           R+L+  V+P+   G  +K W+ +   ++R ++    N    +T    HA D+      + 
Sbjct: 163 RNLLVFVHPKSGKGNGSKVWETVSKIFIRAKV----NTKVIVTERAGHAFDVMASIQNKE 222

Query: 114 A---DAVIAVGGDGTFHEVVNGF 133
               D +IAVGGDG F+E++NG+
Sbjct: 223 LHTYDGIIAVGGDGFFNEILNGY 241

[Close]

BLAST of CSPI06G00780 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 42.7 bits (99), Expect = 6.7e-04
Identity = 23/60 (38.33%), Postives = 36/60 (60.00%), Query Frame = 0


Query: 116 VIAVGGDGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNN-PF 175
           ++  GGDGT      G+       +N DG++ +   +G+IPLGTG+D +R+FGW  + PF
Sbjct: 160 IMVAGGDGTV-----GWVLGCLGELNKDGKS-QIPPVGVIPLGTGNDLSRSFGWGGSFPF 213

The following BLAST results are available for this feature:

BLAST of CSPI06G00780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82359	5.9e-130	64.41	Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana OX=37...	[more]
P9WP28	2.5e-24	27.74	Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) ...	[more]
P9WP29	2.5e-24	27.74	Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) ...	[more]
Q49YU2	3.1e-14	28.30	Diacylglycerol kinase OS=Staphylococcus saprophyticus subsp. saprophyticus (stra...	[more]
Q2YU29	1.5e-13	26.07	Diacylglycerol kinase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) OX=...	[more]

BLAST of CSPI06G00780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BVA2	1.1e-187	89.34	Sphingoid long-chain bases kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3C7U6	1.1e-187	89.34	sphingoid long-chain bases kinase 2, mitochondrial OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1KFE4	5.4e-171	81.64	sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1HNB5	3.5e-170	81.37	sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita moschata OX=3662...	[more]
A0A6J1CZJ4	2.3e-169	79.73	sphingoid long-chain bases kinase 2, mitochondrial OS=Momordica charantia OX=367...	[more]

BLAST of CSPI06G00780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031743330.1	1.1e-189	90.71	sphingoid long-chain bases kinase 2, mitochondrial [Cucumis sativus] >KAE8646552...	[more]
XP_008458171.1	2.3e-187	89.34	PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Cucumis melo] >TY...	[more]
XP_038876180.1	3.9e-179	86.03	sphingoid long-chain bases kinase 2, mitochondrial [Benincasa hispida]	[more]
XP_023514489.1	5.0e-171	81.92	sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita pepo subsp. pepo]	[more]
XP_023000266.1	1.1e-170	81.64	sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita maxima]	[more]

BLAST of CSPI06G00780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46090.1	4.2e-131	64.41	Diacylglycerol kinase family protein	[more]
AT5G23450.1	1.2e-08	26.81	long-chain base (LCB) kinase 1	[more]
AT5G23450.2	1.2e-08	26.81	long-chain base (LCB) kinase 1	[more]
AT5G51290.1	3.9e-04	30.12	Diacylglycerol kinase family protein	[more]
AT2G18730.1	6.7e-04	38.33	diacylglycerol kinase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001206	Diacylglycerol kinase, catalytic domain	SMART	SM00046	dagk_c4a_7	coord: 55..197 e-value: 5.1E-9 score: 46.0
IPR001206	Diacylglycerol kinase, catalytic domain	PFAM	PF00781	DAGK_cat	coord: 56..192 e-value: 5.8E-30 score: 103.5
IPR001206	Diacylglycerol kinase, catalytic domain	PROSITE	PS50146	DAGK	coord: 51..197 score: 34.495937
IPR017438	Inorganic polyphosphate/ATP-NAD kinase, N-terminal	GENE3D	3.40.50.10330		coord: 49..198 e-value: 8.0E-38 score: 131.0
None	No IPR available	GENE3D	2.60.200.40		coord: 199..331 e-value: 3.8E-21 score: 77.6
None	No IPR available	PANTHER	PTHR12358	SPHINGOSINE KINASE	coord: 43..215
None	No IPR available	PANTHER	PTHR12358:SF94	BNAA04G26670D PROTEIN	coord: 220..330 coord: 43..215
None	No IPR available	PANTHER	PTHR12358	SPHINGOSINE KINASE	coord: 220..330
IPR016064	NAD kinase/diacylglycerol kinase-like domain superfamily	SUPERFAMILY	111331	NAD kinase/diacylglycerol kinase-like	coord: 54..338

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G00780.1	CSPI06G00780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0003951	NAD+ kinase activity
molecular_function	GO:0016301	kinase activity