CSPI05G31020 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G31020
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionNB-ARC domain-containing protein
LocationChr5: 28736852 .. 28737285 (-)
RNA-Seq ExpressionCSPI05G31020
SyntenyCSPI05G31020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTTGAGATTGTTAAACCAAGTGAATACCTGGGACGAAGCTAGAAACAGGGTAATTAAATTGGTTGATGATCTTAAAGCTTCTTCTTTACTTCTAGAATCCAATTCAAGGGATAATCATGTTAAAATGCATGATATCGTTCGTGATGTGGCAATATACATTGCATCAAAGGAAGCTAATATGTCTACATTGAGCTATGGTTTCGGGCTGAGTGAATGGCAAGAAAAGGATAGACATGGATCCTATAGAGCAATCTTTGGAAACTGTCACAACTTCTACAACTTTCCTCAAAACTTAGAGTTTCCAAAACTTGAATTGTTGATATTAGATGGACATGATTGGAGGGGAGAAAAACTTCAAATTTGTTACTCTTTTTTTTGAAGGAATGAAAGAACTTAAAGTTTTGAACTTGTCAAGGATGTGTTTTCAAC

mRNA sequence

ATGAGTTTGAGATTGTTAAACCAAGTGAATACCTGGGACGAAGCTAGAAACAGGGTAATTAAATTGGTTGATGATCTTAAAGCTTCTTCTTTACTTCTAGAATCCAATTCAAGGGATAATCATGTTAAAATGCATGATATCGTTCGTGATGTGGCAATATACATTGCATCAAAGGAAGCTAATATGTCTACATTGAGCTATGGTTTCGGGCTGAGTGAATGGCAAGAAAAGGATAGACATGGATCCTATAGAGCAATCTTTGGAAACTGTCACAACTTCTACAACTTTCCTCAAAACTTAGAGTTTCCAAAACTTGAATTGTTGATATTAGATGGACATGATTGGAGGGGAGAAAAACTTCAAATTTGTTACTCTTTTTTTTGAAGGAATGAAAGAACTTAAAGTTTTGAACTTGTCAAGGATGTGTTTTCAAC

Coding sequence (CDS)

ATGAGTTTGAGATTGTTAAACCAAGTGAATACCTGGGACGAAGCTAGAAACAGGGTAATTAAATTGGTTGATGATCTTAAAGCTTCTTCTTTACTTCTAGAATCCAATTCAAGGGATAATCATGTTAAAATGCATGATATCGTTCGTGATGTGGCAATATACATTGCATCAAAGGAAGCTAATATGTCTACATTGAGCTATGGTTTCGGGCTGAGTGAATGGCAAGAAAAGGATAGACATGGATCCTATAGAGCAATCTTTGGAAACTGTCACAACTTCTACAACTTTCCTCAAAACTTAGAGTTTCCAAAACTTGAATTGTTGATATTAGATGGACATGATTGGAGGGGAGAAAAACTTCAAATTTGTTACTCTTTTTTTTGA

Protein sequence

MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASKEANMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDWRGEKLQICYSFF*
Homology
BLAST of CSPI05G31020 vs. ExPASy Swiss-Prot
Match: O81825 (Probable disease resistance protein At4g27220 OS=Arabidopsis thaliana OX=3702 GN=At4g27220 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 3.2e-04
Identity = 36/112 (32.14%), Postives = 58/112 (51.79%), Query Frame = 0

Query: 5   LLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASK--EANM 64
           LL+  + +++  N  + LV+ LK S LL + +S D  VKMHD+VRD AI+  S   E   
Sbjct: 393 LLDGQHHYEDMMNEGVTLVERLKDSCLLEDGDSCDT-VKMHDVVRDFAIWFMSSQGEGFH 452

Query: 65  STLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQN-LEFPKLELLILDGH 114
           S +  G GL E+ +     S + +    +     P N +E  +  +L+L G+
Sbjct: 453 SLVMAGRGLIEFPQDKFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGN 503

BLAST of CSPI05G31020 vs. ExPASy Swiss-Prot
Match: Q9T048 (Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g27190 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.2e-04
Identity = 34/117 (29.06%), Postives = 62/117 (52.99%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYI--ASK 60
           M+   + ++ + +++ N  I  V+ LK   LL + + RD  VKMHD+VRD AI+I  +S+
Sbjct: 420 MAEGFMEELGSQEDSMNEGITTVESLKDYCLLEDGDRRDT-VKMHDVVRDFAIWIMSSSQ 479

Query: 61  EANMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQNLE--FPKLELLILDGH 114
           + + S +  G GL + ++     S R +    +   + P  +E    K  +L+L G+
Sbjct: 480 DDSHSLVMSGTGLQDIRQDKLAPSLRRVSLMNNKLESLPDLVEEFCVKTSVLLLQGN 535

BLAST of CSPI05G31020 vs. ExPASy TrEMBL
Match: A0A6J1DQJ3 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111022191 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.8e-35
Identity = 79/129 (61.24%), Postives = 100/129 (77.52%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASKEA 60
           M + LLN+V+ W+  RN VI+LVD LK+SSLLLESNS DN+V+MHD+VRDVA YIAS E 
Sbjct: 337 MGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIASNEN 396

Query: 61  NMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDW--RGE 120
           NMS+LSYG G SEW+EKDR+GSY AIF +C +F N P+NLEFP L++LIL    +   G+
Sbjct: 397 NMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRIQTFFGEGQ 456

Query: 121 KLQICYSFF 128
            +QI  +FF
Sbjct: 457 AIQIPDTFF 465

BLAST of CSPI05G31020 vs. ExPASy TrEMBL
Match: A0A6J1CPP4 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111013433 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 3.4e-33
Identity = 76/129 (58.91%), Postives = 94/129 (72.87%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASKEA 60
           M + LLN +N W+  +NRVI LVDDLK+S LLLESNS DN+V+MHD+VRDVA YIAS + 
Sbjct: 432 MGMGLLNDINNWEATKNRVIDLVDDLKSSYLLLESNSGDNYVQMHDVVRDVAKYIASNDD 491

Query: 61  NMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDW--RGE 120
            MS+LSYG G SEW E+DR GSY AIF +C +F + P N EFP L+LLIL    +   GE
Sbjct: 492 KMSSLSYGCGQSEWLEEDRSGSYNAIFVDCMDFSSLPSNFEFPNLQLLILGMPSFFGEGE 551

Query: 121 KLQICYSFF 128
            +QI  +FF
Sbjct: 552 AIQIPGAFF 560

BLAST of CSPI05G31020 vs. ExPASy TrEMBL
Match: A0A5D3BQI3 (Disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38G00050 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.5e-33
Identity = 76/131 (58.02%), Postives = 103/131 (78.63%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLL--ESNSRDNHVKMHDIVRDVAIYIASK 60
           M +RLLN+V TW++++NRV+KLV+DL +SSLLL  ES+S+D +VKMH++VRDVAI+IASK
Sbjct: 415 MGMRLLNKVKTWEDSKNRVMKLVNDLISSSLLLEAESDSKDKYVKMHNVVRDVAIHIASK 474

Query: 61  EANMSTLSYGFG-LSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDWRG 120
           E NMST + G+  ++EW+++ R GS+RAIF NC NF N P  + FP+LELLIL   +W  
Sbjct: 475 EGNMSTFNIGYNKVNEWEDEYRSGSHRAIFANCDNFNNLPLKMNFPQLELLILRVSNWLV 534

Query: 121 E-KLQICYSFF 128
           E  LQI Y+FF
Sbjct: 535 ENNLQIPYAFF 545

BLAST of CSPI05G31020 vs. ExPASy TrEMBL
Match: A0A1S3CHN4 (disease resistance protein At4g27190-like OS=Cucumis melo OX=3656 GN=LOC103501064 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.5e-33
Identity = 76/131 (58.02%), Postives = 103/131 (78.63%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLL--ESNSRDNHVKMHDIVRDVAIYIASK 60
           M +RLLN+V TW++++NRV+KLV+DL +SSLLL  ES+S+D +VKMH++VRDVAI+IASK
Sbjct: 415 MGMRLLNKVKTWEDSKNRVMKLVNDLISSSLLLEAESDSKDKYVKMHNVVRDVAIHIASK 474

Query: 61  EANMSTLSYGFG-LSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDWRG 120
           E NMST + G+  ++EW+++ R GS+RAIF NC NF N P  + FP+LELLIL   +W  
Sbjct: 475 EGNMSTFNIGYNKVNEWEDEYRSGSHRAIFANCDNFNNLPLKMNFPQLELLILRVSNWLV 534

Query: 121 E-KLQICYSFF 128
           E  LQI Y+FF
Sbjct: 535 ENNLQIPYAFF 545

BLAST of CSPI05G31020 vs. ExPASy TrEMBL
Match: A0A0A0LTN4 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433370 PE=4 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 7.6e-33
Identity = 77/131 (58.78%), Postives = 102/131 (77.86%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLL--ESNSRDNHVKMHDIVRDVAIYIASK 60
           M +RLLN+V TW++++NRV+KLV+DL +SSLLL  ES+S+D +VKMHD+VRDVAI+IASK
Sbjct: 415 MCMRLLNKVKTWEDSKNRVMKLVNDLISSSLLLEAESDSKDKYVKMHDVVRDVAIHIASK 474

Query: 61  EANMSTLSYGFG-LSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDWRG 120
           E NMSTL+ G+  ++EW+++ R GS+RAIF NC N  N P  + FP+LELLIL    W  
Sbjct: 475 EGNMSTLNIGYNKVNEWEDECRSGSHRAIFANCDNLNNLPLKMNFPQLELLILRVSYWLV 534

Query: 121 E-KLQICYSFF 128
           E  LQI Y+FF
Sbjct: 535 EDNLQIPYAFF 545

BLAST of CSPI05G31020 vs. NCBI nr
Match: KAE8649002.1 (hypothetical protein Csa_008059 [Cucumis sativus])

HSP 1 Score: 265.4 bits (677), Expect = 2.6e-67
Identity = 126/127 (99.21%), Postives = 126/127 (99.21%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASKEA 60
           MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASKEA
Sbjct: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASKEA 60

Query: 61  NMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDWRGEKL 120
           NMSTLSYGFGLSEWQEKDRHG YRAIFGNCHNFYNFPQNLEFPKLELLILDGHDWRGEKL
Sbjct: 61  NMSTLSYGFGLSEWQEKDRHGFYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDWRGEKL 120

Query: 121 QICYSFF 128
           QICYSFF
Sbjct: 121 QICYSFF 127

BLAST of CSPI05G31020 vs. NCBI nr
Match: XP_038890209.1 (probable disease resistance protein At4g27220 [Benincasa hispida])

HSP 1 Score: 256.1 bits (653), Expect = 1.6e-64
Identity = 122/127 (96.06%), Postives = 122/127 (96.06%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASKEA 60
           M LRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHD VRDVAIYIASKEA
Sbjct: 385 MGLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDTVRDVAIYIASKEA 444

Query: 61  NMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDWRGEKL 120
           NMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYN PQNLEFPKLELLILDGHDWRGEKL
Sbjct: 445 NMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNLPQNLEFPKLELLILDGHDWRGEKL 504

Query: 121 QICYSFF 128
           QIC  FF
Sbjct: 505 QICDPFF 511

BLAST of CSPI05G31020 vs. NCBI nr
Match: XP_022155051.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 157.9 bits (398), Expect = 5.8e-35
Identity = 79/129 (61.24%), Postives = 100/129 (77.52%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASKEA 60
           M + LLN+V+ W+  RN VI+LVD LK+SSLLLESNS DN+V+MHD+VRDVA YIAS E 
Sbjct: 337 MGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIASNEN 396

Query: 61  NMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDW--RGE 120
           NMS+LSYG G SEW+EKDR+GSY AIF +C +F N P+NLEFP L++LIL    +   G+
Sbjct: 397 NMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRIQTFFGEGQ 456

Query: 121 KLQICYSFF 128
            +QI  +FF
Sbjct: 457 AIQIPDTFF 465

BLAST of CSPI05G31020 vs. NCBI nr
Match: XP_038902150.1 (disease resistance protein At4g27190-like [Benincasa hispida])

HSP 1 Score: 154.5 bits (389), Expect = 6.4e-34
Identity = 77/130 (59.23%), Postives = 101/130 (77.69%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLE--SNSRDNHVKMHDIVRDVAIYIASK 60
           M +RLLN+V TW++A+NRV+KLV DL +SSL LE  S+S+D +VKMHD+VRDVAI+IAS 
Sbjct: 415 MGMRLLNKVKTWEDAKNRVMKLVHDLISSSLFLEADSDSKDKYVKMHDVVRDVAIHIASN 474

Query: 61  EANMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDWRGE 120
           E NMSTL+ G+ L+EW+++ R GS+RAIF NC N  N P  ++FP+LELLIL   +W  E
Sbjct: 475 EGNMSTLNIGYKLNEWEDEYRSGSHRAIFANCDNLNNLPPKMKFPQLELLILRVSNWLVE 534

Query: 121 -KLQICYSFF 128
             LQI Y+FF
Sbjct: 535 NNLQIPYAFF 544

BLAST of CSPI05G31020 vs. NCBI nr
Match: XP_022143564.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 151.0 bits (380), Expect = 7.0e-33
Identity = 76/129 (58.91%), Postives = 94/129 (72.87%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASKEA 60
           M + LLN +N W+  +NRVI LVDDLK+S LLLESNS DN+V+MHD+VRDVA YIAS + 
Sbjct: 432 MGMGLLNDINNWEATKNRVIDLVDDLKSSYLLLESNSGDNYVQMHDVVRDVAKYIASNDD 491

Query: 61  NMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQNLEFPKLELLILDGHDW--RGE 120
            MS+LSYG G SEW E+DR GSY AIF +C +F + P N EFP L+LLIL    +   GE
Sbjct: 492 KMSSLSYGCGQSEWLEEDRSGSYNAIFVDCMDFSSLPSNFEFPNLQLLILGMPSFFGEGE 551

Query: 121 KLQICYSFF 128
            +QI  +FF
Sbjct: 552 AIQIPGAFF 560

BLAST of CSPI05G31020 vs. TAIR 10
Match: AT4G27220.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 46.2 bits (108), Expect = 2.3e-05
Identity = 36/112 (32.14%), Postives = 58/112 (51.79%), Query Frame = 0

Query: 5   LLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYIASK--EANM 64
           LL+  + +++  N  + LV+ LK S LL + +S D  VKMHD+VRD AI+  S   E   
Sbjct: 393 LLDGQHHYEDMMNEGVTLVERLKDSCLLEDGDSCDT-VKMHDVVRDFAIWFMSSQGEGFH 452

Query: 65  STLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQN-LEFPKLELLILDGH 114
           S +  G GL E+ +     S + +    +     P N +E  +  +L+L G+
Sbjct: 453 SLVMAGRGLIEFPQDKFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGN 503

BLAST of CSPI05G31020 vs. TAIR 10
Match: AT4G27190.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 45.8 bits (107), Expect = 3.0e-05
Identity = 34/117 (29.06%), Postives = 62/117 (52.99%), Query Frame = 0

Query: 1   MSLRLLNQVNTWDEARNRVIKLVDDLKASSLLLESNSRDNHVKMHDIVRDVAIYI--ASK 60
           M+   + ++ + +++ N  I  V+ LK   LL + + RD  VKMHD+VRD AI+I  +S+
Sbjct: 420 MAEGFMEELGSQEDSMNEGITTVESLKDYCLLEDGDRRDT-VKMHDVVRDFAIWIMSSSQ 479

Query: 61  EANMSTLSYGFGLSEWQEKDRHGSYRAIFGNCHNFYNFPQNLE--FPKLELLILDGH 114
           + + S +  G GL + ++     S R +    +   + P  +E    K  +L+L G+
Sbjct: 480 DDSHSLVMSGTGLQDIRQDKLAPSLRRVSLMNNKLESLPDLVEEFCVKTSVLLLQGN 535

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818253.2e-0432.14Probable disease resistance protein At4g27220 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9T0484.2e-0429.06Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g2719... [more]
Match NameE-valueIdentityDescription
A0A6J1DQJ32.8e-3561.24probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1CPP43.4e-3358.91probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=... [more]
A0A5D3BQI34.5e-3358.02Disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3CHN44.5e-3358.02disease resistance protein At4g27190-like OS=Cucumis melo OX=3656 GN=LOC10350106... [more]
A0A0A0LTN47.6e-3358.78NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433370 PE=4... [more]
Match NameE-valueIdentityDescription
KAE8649002.12.6e-6799.21hypothetical protein Csa_008059 [Cucumis sativus][more]
XP_038890209.11.6e-6496.06probable disease resistance protein At4g27220 [Benincasa hispida][more]
XP_022155051.15.8e-3561.24probable disease resistance protein At4g27220 [Momordica charantia][more]
XP_038902150.16.4e-3459.23disease resistance protein At4g27190-like [Benincasa hispida][more]
XP_022143564.17.0e-3358.91probable disease resistance protein At4g27220 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G27220.12.3e-0532.14NB-ARC domain-containing disease resistance protein [more]
AT4G27190.13.0e-0529.06NB-ARC domain-containing disease resistance protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33463FAMILY NOT NAMEDcoord: 4..113
NoneNo IPR availablePANTHERPTHR33463:SF50DISEASE RESISTANCE RPS5-LIKE PROTEINcoord: 4..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G31020.1CSPI05G31020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding