CSPI05G30790 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G30790
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationChr5: 28569818 .. 28572627 (+)
RNA-Seq ExpressionCSPI05G30790
SyntenyCSPI05G30790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAGGTGGTTGATATGGGCACTTTTCTTCCTCATAAGTACCCTTTCCGCCTCTTCATCTTCTCCGGCACCGGCCGCTTTCGTAGGAGTTCAAGACGGACATTTTCAGCTAAATGGTTCTCCATTTTTGTTCAATGGCTTTAACTCTTACTGGATGATGTCCGTCGCTACTGACCCGAACCAACGCAACAAAGTAACCCAAGTCTTTCGTGATGCCGCCGCTGCCGGCCTCACCGTCTGCCGAACCTGGGCCTTCAACGACGGTGGCTTCCATGCCCTCCAAATCTCTCCCGGCGTTTATGATGAATCAGTTTTTCAGGTTAACTTTTTACTAATTTTCTTTTCTTCTTTTACTCTCTCAAATATGTAATTCCATTACTTAAAAAAAAAGTAATACTTCGATCCATCTTCAATTACAAACGAAAAATTATATTTTCTTAATAACAAAAAAAAAAAAAATAAATAAATATTAATTAAATACAGAAAATAAACAAAGAAATAATTGTTACCAAGCGATTCTAAATTCTATAATTTAAAATGTTTATAAACATATTATTAAATGAACTCTTTGTTGAAATATAAGAATTAAAAGTTCATCAAATCATATAGATTATTTACGAGGTTTACAAGAATCAAAATCTACAATCTTTTGATGACTTTTAATTTAGAATATTGTTTGACTTCAGGGTCTTGATTTCGTGATATTTGAAGCAAGAAAGTATGGAATTAGAATGATATTGAGTTTGGTAAATAATTTCAAAGACTACGGCGGAAGAGCAGCGTATGTCCGGTGGGCCGAAGCCGCCGGCGTTCAAGTTCATGACGAAGATGACTTCTACACTAATCAACTTATCAAAACATACTACAAAAACCATGTTCAGGTGATCAAATCTATACCTAAAACTAGCTACTATATTATTATTGGACTTGAAAACTAAACTAAAAATCAAACAGAAATCAATAATATTACTTCTATTTTGTTTTTGTAGAAAGTATTGAGCAGAAAGAACACAATGAATGGTTTGATATATATGGAAGATGCAACCATTATGGGATGGGAACTTATGAACGAACCACGTTGTCAAGTTGATTCCTCCGGCAACACTGTCAATGTAACCTTTCATTATTTAACTTCTCTCTTTTTTCTCTTTTCCTTAATTAATCACTTTCATTTTTAGTGCCTAATCCTATTTTTATAACCTAAATTTTCATTACAAAGAAATCGAGGAAAATCTATGGAGATTCAAGTCCAAATAAAATCAATCGAACACGCTTAGAATGACATATTGATTGTTGACTTAATTATTACTGTACATAGAATTTGTTAGTACATGTTGCATTATATAGCATTATATTTAAGTCCAACTATCATTTCTAAAGATTTTTTTATGGTTTATCAAAAATTAAAGGTGTCATTTCTAGTATTAGTTCTCGTTACATTGTTGATAGCTTTTAATTTAAGAAATGAGCTATTAGTTTATATTATTGATAACGACCATTGGTGATTGTTTCTATCGGTCATAGACATGTGCCTTTATTTTTAAAAAAAATTATACACTTATATGTAATGGTCGTTTCCTAATGAAAATAGAGATGGGTAGAGGAGATGGGATCATATGTGAAATCAATTGACAAACAACATTTGGTGGGGATTGGAATGGAAGGATTCTATGGAGATTCAAGTCCAAATAAAATCAAAGCCAACCCAGGTAGTTTCAAATTTGGCACTGATTTTATCACCAATAACCTCAACAAAGCCATCGACTTTGCAACAATTCATGCATATCCTGATGCTTGGTTAGTTTCTTCTTCCTCTTATCTCCTATCCTCTATTCTTATTACTTTATACGTATTTTTCTATACACAGAAATTTGAAATTATGACCTCAAAAAATATAGTATGATAGCTAAAGTTGAATCATGTACTCACTTTGATAAATTTTGTTAAAGTTAGATGGTATGTTACTAAGAAGAGTGTTTATTAACATCTTCAGCATGTTGGAGTACATAACACTTGAAAAAAAGAACAAAAAAACAAATAGATTTTGACAACTGACTAGTGCAATTAATTTGTTAATTGGCAAGTTAAATTTCATTCGTAGATCTAGAAAGTTTTCAATCGTTGTTTTCGTAAAGATATAATTGAGAAATATTAATAGTAACCCTAAAATCTAGAAATGAAAGGTTTGATTCCGTTGAGTTTTAAAGGTCCATGAACTCCTTAAGTTTACTACTTCTTCACTCCCCCACCTTCCACAAGTCGAACATTAATTAGATGAATGTTTTTAGCCTAAAAATGCATTGTAAGTAAAAAGCCAAAACATAGTGAGGGAAGAAAATGGTAAAAACATAAATAAAAAATTAAAACATGCAAGAAAGAAAATCAAGAGAGCCTTTAAATTTGTTTTCCATCGTTAATTATAGGCTACCAGGGAAGAGTGAAGGCACCAAAATGGCATTCCTTGAAGAATGGATAGCACTTCACTGGACGGACTCAAAGACTATCTTAAAGAAGCCATTGATTTTTGAAGAATTTGGAAAGTCGATCAGAGACCAAAACCAAACATCTAGTAGTGCAAGAGACAGAGATGCCTTCTTGAGTAAGGTTTTTTCAATTATCTACAACCTTGCAAGAAATGGAGCAACCATGGCTGGAGGTCTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCGTATTACGATGGATATGAGATCGTTCTTTCCCAGAGTCCTTCAACAACCGCCATTATAACACATCAGTCAAACAATATGGCTGCACTAAATACTAGAACTCAACACCACCTCTGA

mRNA sequence

ATGATGAGGTGGTTGATATGGGCACTTTTCTTCCTCATAAGTACCCTTTCCGCCTCTTCATCTTCTCCGGCACCGGCCGCTTTCGTAGGAGTTCAAGACGGACATTTTCAGCTAAATGGTTCTCCATTTTTGTTCAATGGCTTTAACTCTTACTGGATGATGTCCGTCGCTACTGACCCGAACCAACGCAACAAAGTAACCCAAGTCTTTCGTGATGCCGCCGCTGCCGGCCTCACCGTCTGCCGAACCTGGGCCTTCAACGACGGTGGCTTCCATGCCCTCCAAATCTCTCCCGGCGTTTATGATGAATCAGTTTTTCAGGGTCTTGATTTCGTGATATTTGAAGCAAGAAAGTATGGAATTAGAATGATATTGAGTTTGGTAAATAATTTCAAAGACTACGGCGGAAGAGCAGCGTATGTCCGGTGGGCCGAAGCCGCCGGCGTTCAAGTTCATGACGAAGATGACTTCTACACTAATCAACTTATCAAAACATACTACAAAAACCATGTTCAGAAAGTATTGAGCAGAAAGAACACAATGAATGGTTTGATATATATGGAAGATGCAACCATTATGGGATGGGAACTTATGAACGAACCACGTTGTCAAGTTGATTCCTCCGGCAACACTGTCAATAGATGGGTAGAGGAGATGGGATCATATGTGAAATCAATTGACAAACAACATTTGGTGGGGATTGGAATGGAAGGATTCTATGGAGATTCAAGTCCAAATAAAATCAAAGCCAACCCAGGTAGTTTCAAATTTGGCACTGATTTTATCACCAATAACCTCAACAAAGCCATCGACTTTGCAACAATTCATGCATATCCTGATGCTTGGCTACCAGGGAAGAGTGAAGGCACCAAAATGGCATTCCTTGAAGAATGGATAGCACTTCACTGGACGGACTCAAAGACTATCTTAAAGAAGCCATTGATTTTTGAAGAATTTGGAAAGTCGATCAGAGACCAAAACCAAACATCTAGTAGTGCAAGAGACAGAGATGCCTTCTTGAGTAAGGTTTTTTCAATTATCTACAACCTTGCAAGAAATGGAGCAACCATGGCTGGAGGTCTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCGTATTACGATGGATATGAGATCGTTCTTTCCCAGAGTCCTTCAACAACCGCCATTATAACACATCAGTCAAACAATATGGCTGCACTAAATACTAGAACTCAACACCACCTCTGA

Coding sequence (CDS)

ATGATGAGGTGGTTGATATGGGCACTTTTCTTCCTCATAAGTACCCTTTCCGCCTCTTCATCTTCTCCGGCACCGGCCGCTTTCGTAGGAGTTCAAGACGGACATTTTCAGCTAAATGGTTCTCCATTTTTGTTCAATGGCTTTAACTCTTACTGGATGATGTCCGTCGCTACTGACCCGAACCAACGCAACAAAGTAACCCAAGTCTTTCGTGATGCCGCCGCTGCCGGCCTCACCGTCTGCCGAACCTGGGCCTTCAACGACGGTGGCTTCCATGCCCTCCAAATCTCTCCCGGCGTTTATGATGAATCAGTTTTTCAGGGTCTTGATTTCGTGATATTTGAAGCAAGAAAGTATGGAATTAGAATGATATTGAGTTTGGTAAATAATTTCAAAGACTACGGCGGAAGAGCAGCGTATGTCCGGTGGGCCGAAGCCGCCGGCGTTCAAGTTCATGACGAAGATGACTTCTACACTAATCAACTTATCAAAACATACTACAAAAACCATGTTCAGAAAGTATTGAGCAGAAAGAACACAATGAATGGTTTGATATATATGGAAGATGCAACCATTATGGGATGGGAACTTATGAACGAACCACGTTGTCAAGTTGATTCCTCCGGCAACACTGTCAATAGATGGGTAGAGGAGATGGGATCATATGTGAAATCAATTGACAAACAACATTTGGTGGGGATTGGAATGGAAGGATTCTATGGAGATTCAAGTCCAAATAAAATCAAAGCCAACCCAGGTAGTTTCAAATTTGGCACTGATTTTATCACCAATAACCTCAACAAAGCCATCGACTTTGCAACAATTCATGCATATCCTGATGCTTGGCTACCAGGGAAGAGTGAAGGCACCAAAATGGCATTCCTTGAAGAATGGATAGCACTTCACTGGACGGACTCAAAGACTATCTTAAAGAAGCCATTGATTTTTGAAGAATTTGGAAAGTCGATCAGAGACCAAAACCAAACATCTAGTAGTGCAAGAGACAGAGATGCCTTCTTGAGTAAGGTTTTTTCAATTATCTACAACCTTGCAAGAAATGGAGCAACCATGGCTGGAGGTCTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCGTATTACGATGGATATGAGATCGTTCTTTCCCAGAGTCCTTCAACAACCGCCATTATAACACATCAGTCAAACAATATGGCTGCACTAAATACTAGAACTCAACACCACCTCTGA

Protein sequence

MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNTMNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIALHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQHHL*
Homology
BLAST of CSPI05G30790 vs. ExPASy Swiss-Prot
Match: Q9FZ03 (Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 493.4 bits (1269), Expect = 2.4e-138
Identity = 228/374 (60.96%), Postives = 293/374 (78.34%), Query Frame = 0

Query: 28  FVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAAAAGLTVCRTWAFN 87
           FV V   HF+LNGSPFLFNGFNSYW+M VA +P++R KV++V R+A++AGL+VCRTWAF+
Sbjct: 39  FVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFS 98

Query: 88  DGGFHALQISPGVYDESVFQGLDFVIFEARKYGIRMILSLVNNFKDYGGRAAYVRWAEAA 147
           DGG  ALQISPGVYDE VFQGLDFVI EA+KYGIR+ILS VNN+ D+GG+A YV+WA  A
Sbjct: 99  DGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNA 158

Query: 148 GVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNTMNGLIYMEDATIMGWELMNEPRCQVDS 207
           G Q++ +DDFYTN + K YYKNH++KV++R NT+ G+ Y +D+TIM WELMNEPR Q D 
Sbjct: 159 GAQINGDDDFYTNYITKNYYKNHIKKVVTRFNTITGMTYKDDSTIMAWELMNEPRNQADY 218

Query: 208 SGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLN 267
           SGNT+N WV+EM S+VKS+D +HL+ IGMEGFYGDS P +   NPG ++ GTDFI+N+L 
Sbjct: 219 SGNTLNAWVQEMASFVKSLDNKHLLEIGMEGFYGDSVPERKSINPG-YQVGTDFISNHLI 278

Query: 268 KAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIALHWTDSKTILKKPLIFEEFGKSIRDQN 327
           K IDFATIHAY D WL G+S+  +M F+++W+  HW D+K ILKKPL+  EFGKS RD  
Sbjct: 279 KEIDFATIHAYTDQWLSGQSDDAQMIFMQKWMTSHWQDAKNILKKPLVLAEFGKSSRDPG 338

Query: 328 QTSSSARDRDAFLSKVFSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQSPST 387
              +    RD F+S ++  IY+LA++G TM G L+WQ++A+GME+Y DGY I L ++PST
Sbjct: 339 YNQNI---RDTFMSTIYRNIYSLAKDGGTMGGSLIWQLVAQGMENYEDGYCIELGKNPST 398

Query: 388 TAIITHQSNNMAAL 402
             IIT QS+ M AL
Sbjct: 399 AGIITSQSHAMTAL 408

BLAST of CSPI05G30790 vs. ExPASy Swiss-Prot
Match: Q6YM50 (Mannan endo-1,4-beta-mannosidase 5 OS=Solanum lycopersicum OX=4081 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 2.4e-138
Identity = 228/374 (60.96%), Postives = 293/374 (78.34%), Query Frame = 0

Query: 28  FVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAAAAGLTVCRTWAFN 87
           FV V   HF+LNGSPFLFNGFNSYW+M VA +P++R KV++V R+A++AGL+VCRTWAF+
Sbjct: 39  FVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFS 98

Query: 88  DGGFHALQISPGVYDESVFQGLDFVIFEARKYGIRMILSLVNNFKDYGGRAAYVRWAEAA 147
           DGG  ALQISPGVYDE VFQGLDFVI EA+KYGIR+ILS VNN+ D+GG+A YV+WA  A
Sbjct: 99  DGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNA 158

Query: 148 GVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNTMNGLIYMEDATIMGWELMNEPRCQVDS 207
           G Q++ +DDFYTN + K YYKNH++KV++R NT+ G+ Y +D+TIM WELMNEPR Q D 
Sbjct: 159 GAQINGDDDFYTNYITKNYYKNHIKKVVTRFNTITGMTYKDDSTIMAWELMNEPRNQADY 218

Query: 208 SGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFYGDSSPNKIKANPGSFKFGTDFITNNLN 267
           SGNT+N WV+EM S+VKS+D +HL+ IGMEGFYGDS P +   NPG ++ GTDFI+N+L 
Sbjct: 219 SGNTLNAWVQEMASFVKSLDNKHLLEIGMEGFYGDSVPERKSINPG-YQVGTDFISNHLI 278

Query: 268 KAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIALHWTDSKTILKKPLIFEEFGKSIRDQN 327
           K IDFATIHAY D WL G+S+  +M F+++W+  HW D+K ILKKPL+  EFGKS RD  
Sbjct: 279 KEIDFATIHAYTDQWLSGQSDDAQMIFMQKWMTSHWQDAKNILKKPLVLAEFGKSSRDPG 338

Query: 328 QTSSSARDRDAFLSKVFSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQSPST 387
              +    RD F+S ++  IY+LA++G TM G L+WQ++A+GME+Y DGY I L ++PST
Sbjct: 339 YNQNI---RDTFMSTIYRNIYSLAKDGGTMGGSLIWQLVAQGMENYEDGYCIELGKNPST 398

Query: 388 TAIITHQSNNMAAL 402
             IIT QS+ M AL
Sbjct: 399 AGIITSQSHAMTAL 408

BLAST of CSPI05G30790 vs. ExPASy Swiss-Prot
Match: O48540 (Mannan endo-1,4-beta-mannosidase 1 OS=Solanum lycopersicum OX=4081 GN=MAN1 PE=1 SV=2)

HSP 1 Score: 486.1 bits (1250), Expect = 3.9e-136
Identity = 232/399 (58.15%), Postives = 297/399 (74.44%), Query Frame = 0

Query: 3   RWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDPNQ 62
           R  I  LF L   L   ++S     F+GV+D HF+LNGSPFLFNGFNSYW+M VA DP +
Sbjct: 6   RSCICGLFLLFLALVCEANS----GFIGVKDSHFELNGSPFLFNGFNSYWLMHVAADPTE 65

Query: 63  RNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYGIR 122
           R KVT+V +DA+ AGL+VCRTWAF+DGG  ALQISPG+YDE VFQGLDFVI EA+KYGIR
Sbjct: 66  RYKVTEVLKDASVAGLSVCRTWAFSDGGDRALQISPGIYDERVFQGLDFVIAEAKKYGIR 125

Query: 123 MILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNTMN 182
           +ILS VN + D+GG+A YV WA  AG Q+ ++D+FYT+ ++K Y KNH++KV++R N++ 
Sbjct: 126 LILSFVNQWNDFGGKAQYVWWARNAGAQISNDDEFYTHPMLKKYLKNHIEKVVTRLNSIT 185

Query: 183 GLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFYGD 242
            + Y +D TIM WELMNEPR Q D SG TVN WV+EM S+VKS+D +HL+ +GMEGFYGD
Sbjct: 186 KVAYKDDPTIMAWELMNEPRDQADYSGKTVNGWVQEMASFVKSLDNKHLLEVGMEGFYGD 245

Query: 243 SSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIALH 302
           S P +   NPG ++ GTDFI+N+L   IDFATIHAY D W+ G+S+  ++ ++E+WI  H
Sbjct: 246 SIPERKSVNPG-YQVGTDFISNHLINEIDFATIHAYTDQWVSGQSDDAQLVWMEKWITSH 305

Query: 303 WTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGGLV 362
           W D++ ILKKPL+  EFGKS R Q         RD F+S V+  +YNLA+ G TMAG LV
Sbjct: 306 WEDARNILKKPLVLAEFGKSSRGQG-------SRDIFMSSVYRNVYNLAKEGGTMAGSLV 365

Query: 363 WQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAAL 402
           WQ+MA GME+Y DGY IVL Q+PSTT II+ Q++ M AL
Sbjct: 366 WQLMAHGMENYDDGYCIVLGQTPSTTQIISDQAHVMTAL 392

BLAST of CSPI05G30790 vs. ExPASy Swiss-Prot
Match: Q9FUQ6 (Mannan endo-1,4-beta-mannosidase 3 OS=Solanum lycopersicum OX=4081 GN=MAN3 PE=3 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 6.2e-134
Identity = 235/406 (57.88%), Postives = 301/406 (74.14%), Query Frame = 0

Query: 3   RWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDPNQ 62
           R  I  LF L+  LS  ++S     F+GV+D HF+LNGSPFLFNGFNSYW+M VA DP +
Sbjct: 7   RSCISGLFLLLLALSCEANS----GFIGVKDSHFELNGSPFLFNGFNSYWLMHVAADPAE 66

Query: 63  RNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYGIR 122
           R KVT+V +DA+ AGL+VCRTWAF+DGG  ALQISPGVYDE VFQGLDFVI EA+KYG+R
Sbjct: 67  RYKVTEVLKDASTAGLSVCRTWAFSDGGDRALQISPGVYDERVFQGLDFVIAEAKKYGVR 126

Query: 123 MILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNTMN 182
           +ILS VN + D+GG+A YV WA  AGVQ+ ++D+FYT+ ++K Y KNH++ V++R N++ 
Sbjct: 127 LILSFVNQWNDFGGKAEYVWWARNAGVQISNDDEFYTHPILKKYLKNHIE-VVTRLNSIT 186

Query: 183 GLIYMEDATIMGWELMNEPRCQVDSSGNTVN-RWVEEMGSYVKSIDKQHLVGIGMEGFYG 242
            + Y +DATIM WELMNEPR Q D SG TVN  WV+EM S+VKS+D +HL+ +GMEGFYG
Sbjct: 187 KVAYKDDATIMAWELMNEPRDQADYSGKTVNVGWVQEMASFVKSLDNKHLLEVGMEGFYG 246

Query: 243 DSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIAL 302
           DS P +   NPG ++ GTDFI+N+L   IDFATIHAY D WL G+S+  ++A++E+WI  
Sbjct: 247 DSIPERKLVNPG-YQVGTDFISNHLINEIDFATIHAYTDQWLSGQSDEAQLAWMEKWIRS 306

Query: 303 HWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGGL 362
           HW D++ ILKKPL+  EFGKS R      S    RD F+S V+  +YNLA+ G TM G L
Sbjct: 307 HWEDARNILKKPLVLAEFGKSSR------SGEGSRDIFMSSVYRNVYNLAKEGGTMGGSL 366

Query: 363 VWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQH 408
           VWQ+MA GME+Y DGY IVL  +PSTT II++Q++ M AL     H
Sbjct: 367 VWQLMAHGMENYDDGYSIVLGLNPSTTQIISNQAHIMTALAHSLNH 400

BLAST of CSPI05G30790 vs. ExPASy Swiss-Prot
Match: Q0JKM9 (Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN1 PE=2 SV=2)

HSP 1 Score: 421.8 bits (1083), Expect = 9.0e-117
Identity = 197/393 (50.13%), Postives = 271/393 (68.96%), Query Frame = 0

Query: 12  LISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFR 71
           +I  L  + +      FV  Q   F L+G+P+  NGFN+YW+M +A DP+QR KV+    
Sbjct: 21  VIHGLGEAEALGGGGGFVRAQGTRFVLDGNPYYANGFNAYWLMLLAADPSQRGKVSAALG 80

Query: 72  DAAAAGLTVCRTWAFND-GGFHALQISPGVYDESVFQGLDFVIFEARKYGIRMILSLVNN 131
           +AA  GLTV RTWAF+D GG +ALQ+SPG Y+E+ F+GLDFV+ EARKYGI++ILSLV+N
Sbjct: 81  EAAGHGLTVARTWAFSDGGGGNALQLSPGNYNENTFKGLDFVLSEARKYGIKVILSLVDN 140

Query: 132 FKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNTMNGLIYMEDA 191
           +  +GGR  YV WA A G  +  +D+F+TN ++K +YKNHV+ VL+RKNT+ G+ Y +D 
Sbjct: 141 YDSFGGRKQYVNWARAQGQGIGSDDEFFTNPVVKGFYKNHVKTVLTRKNTITGVAYRDDP 200

Query: 192 TIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFYGDSSPNKIKA 251
           TI+ WELMNEPRCQ D SG TV  W+ EM ++VKSID+ H++ +G+EGFYG SSP++I A
Sbjct: 201 TILAWELMNEPRCQSDLSGRTVQSWITEMAAHVKSIDRNHMLEVGLEGFYGASSPSRIAA 260

Query: 252 -NPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIALHWTDSKTI 311
            NP  ++ GTDFI NN    IDFAT+H+YPD WL GK +  ++ F+  W+  H  D++ +
Sbjct: 261 VNPSGYQLGTDFIANNQVPGIDFATVHSYPDQWLSGKDDQAQLGFMGRWLDAHIADAQAV 320

Query: 312 LKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGGLVWQVMAEG 371
           L+KPL+  EFGKS +D   +S     RDA    V++ IY  AR G    GGL WQ++  G
Sbjct: 321 LRKPLLIAEFGKSWKDPGYSSG---QRDALYGTVYAKIYESARRGGATVGGLFWQLLVPG 380

Query: 372 MESYYDGYEIVLSQSPSTTAIITHQSNNMAALN 403
           M+SY DGYE+V  ++PSTT +IT  S  +  L+
Sbjct: 381 MDSYRDGYEVVFGETPSTTGVITTNSRRLRFLS 410

BLAST of CSPI05G30790 vs. ExPASy TrEMBL
Match: A0A0A0KSS2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G648710 PE=3 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 9.6e-239
Identity = 407/409 (99.51%), Postives = 408/409 (99.76%), Query Frame = 0

Query: 1   MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP 60
           MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP
Sbjct: 8   MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP 67

Query: 61  NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 120
           NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG
Sbjct: 68  NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 127

Query: 121 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT 180
           IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT
Sbjct: 128 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT 187

Query: 181 MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY 240
           MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY
Sbjct: 188 MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY 247

Query: 241 GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA 300
           GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA
Sbjct: 248 GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA 307

Query: 301 LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGG 360
           LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSS RDRDAFLSKVFSIIYNLARNGATMAGG
Sbjct: 308 LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSVRDRDAFLSKVFSIIYNLARNGATMAGG 367

Query: 361 LVWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQHHL 410
           LVWQVMAEGMESYYDGYEIVLSQ+PSTTAIITHQSNNMAALNTRTQHHL
Sbjct: 368 LVWQVMAEGMESYYDGYEIVLSQTPSTTAIITHQSNNMAALNTRTQHHL 416

BLAST of CSPI05G30790 vs. ExPASy TrEMBL
Match: A0A5D3BKZ3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00890 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.1e-213
Identity = 367/409 (89.73%), Postives = 383/409 (93.64%), Query Frame = 0

Query: 1   MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP 60
           M+RWLIW L  LISTLS SSSSP  A FVGV+DGHFQLNGSPFLFNGFNSYWMMSVA DP
Sbjct: 7   MIRWLIWGLLLLISTLS-SSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAADP 66

Query: 61  NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 120
           NQR+KV+QVFRDAA AGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG
Sbjct: 67  NQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 126

Query: 121 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT 180
           IRMILSLVNNFKDYGGRAAYVRWAEAAGVQV +EDDFYTNQLI+TYYKNHVQKVL RKNT
Sbjct: 127 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKNT 186

Query: 181 MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY 240
           M+ L YM+D TIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSID+QHLVGIGMEGFY
Sbjct: 187 MSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGFY 246

Query: 241 GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA 300
           GDS P KIKANP S+K GTDFITNNLNKAID+ATIHAYPDAWLPGKSE TKMAFLEEW+A
Sbjct: 247 GDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWVA 306

Query: 301 LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGG 360
           LHWTDSKTILKKPLIFEEFG+SIRDQNQT  S RDRDAFLSKV+SIIYNLA+NGATMAGG
Sbjct: 307 LHWTDSKTILKKPLIFEEFGRSIRDQNQT-YSVRDRDAFLSKVYSIIYNLAKNGATMAGG 366

Query: 361 LVWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQHHL 410
           LVWQVMAEGMESYYDGYEIVLSQ+PST AIIT QSN MAAL TRTQHHL
Sbjct: 367 LVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALKTRTQHHL 413

BLAST of CSPI05G30790 vs. ExPASy TrEMBL
Match: A0A1S3CB04 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103498662 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.1e-213
Identity = 367/409 (89.73%), Postives = 383/409 (93.64%), Query Frame = 0

Query: 1   MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP 60
           M+RWLIW L  LISTLS SSSSP  A FVGV+DGHFQLNGSPFLFNGFNSYWMMSVA DP
Sbjct: 7   MIRWLIWGLLLLISTLS-SSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAADP 66

Query: 61  NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 120
           NQR+KV+QVFRDAA AGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG
Sbjct: 67  NQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 126

Query: 121 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT 180
           IRMILSLVNNFKDYGGRAAYVRWAEAAGVQV +EDDFYTNQLI+TYYKNHVQKVL RKNT
Sbjct: 127 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKNT 186

Query: 181 MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY 240
           M+ L YM+D TIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSID+QHLVGIGMEGFY
Sbjct: 187 MSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGFY 246

Query: 241 GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA 300
           GDS P KIKANP S+K GTDFITNNLNKAID+ATIHAYPDAWLPGKSE TKMAFLEEW+A
Sbjct: 247 GDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWVA 306

Query: 301 LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGG 360
           LHWTDSKTILKKPLIFEEFG+SIRDQNQT  S RDRDAFLSKV+SIIYNLA+NGATMAGG
Sbjct: 307 LHWTDSKTILKKPLIFEEFGRSIRDQNQT-YSVRDRDAFLSKVYSIIYNLAKNGATMAGG 366

Query: 361 LVWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQHHL 410
           LVWQVMAEGMESYYDGYEIVLSQ+PST AIIT QSN MAAL TRTQHHL
Sbjct: 367 LVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALKTRTQHHL 413

BLAST of CSPI05G30790 vs. ExPASy TrEMBL
Match: A0A5A7TCI2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G00910 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.8e-190
Identity = 339/409 (82.89%), Postives = 355/409 (86.80%), Query Frame = 0

Query: 1   MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP 60
           M+RWLIW L  LISTLS SSSSP  A FVGV+DGHFQLNGSPFLFNGFNSYWMMSVA DP
Sbjct: 7   MIRWLIWGLLLLISTLS-SSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAADP 66

Query: 61  NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 120
           NQR+KV+QVFRDAA AGLT                            GLDFVIFEARKYG
Sbjct: 67  NQRHKVSQVFRDAATAGLT----------------------------GLDFVIFEARKYG 126

Query: 121 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT 180
           IRMILSLVNNFKDYGGRAAYVRWAEAAGVQV +EDDFYTNQLI+TYYKNHVQKVL RKNT
Sbjct: 127 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKNT 186

Query: 181 MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY 240
           M+ L YM+D TIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSID+QHLVGIGMEGFY
Sbjct: 187 MSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGFY 246

Query: 241 GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA 300
           GDS P KIKANP S+K GTDFITNNLNKAID+ATIHAYPDAWLPGKSE TKMAFLEEW+A
Sbjct: 247 GDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWVA 306

Query: 301 LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGG 360
           LHWTDSKTILKKPLIFEEFG+SIRDQNQT  S RDRDAFLSKV+SIIYNLA+NGATMAGG
Sbjct: 307 LHWTDSKTILKKPLIFEEFGRSIRDQNQT-YSVRDRDAFLSKVYSIIYNLAKNGATMAGG 366

Query: 361 LVWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQHHL 410
           LVWQVMAEGMESYYDGYEIVLSQ+PST AIIT QSN MAAL TRTQHHL
Sbjct: 367 LVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALKTRTQHHL 385

BLAST of CSPI05G30790 vs. ExPASy TrEMBL
Match: A0A6J1CK63 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111012035 PE=3 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.6e-185
Identity = 313/403 (77.67%), Postives = 354/403 (87.84%), Query Frame = 0

Query: 2   MRWLIWALFFLI--STLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATD 61
           MRWL+W +  ++     S+SS +PAPAAFVG+ DGHFQLNGSPF+FNGFNSYWMMSV  D
Sbjct: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60

Query: 62  PNQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 121
           PNQR KV+  FRDAAAAGLTVCRTWAF DGG+HALQISPGVYDESVFQ LDFVI+EARKY
Sbjct: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120

Query: 122 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKN 181
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+H++DDFYTNQLIK +Y  HV+KVL+RKN
Sbjct: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180

Query: 182 TMNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGF 241
           TM+GL YM+D TIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSIDK+HLVGIGMEGF
Sbjct: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240

Query: 242 YGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWI 301
           YGDS+P++IK NP  +K GTDF+TNNL K IDFATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300

Query: 302 ALHWTDSKTILKKPLIFEEFGKSIRDQNQTSS-SARDRDAFLSKVFSIIYNLARNGATMA 361
            +HW+DSKTILKKPLIF EFGKS ++QNQ  + S RDRD+FL+KV+S IYNLARNG TMA
Sbjct: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360

Query: 362 GGLVWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAAL 402
           GGLVWQVMAEGMESYYDGYEIVLSQ+P TTAII  QS NMAAL
Sbjct: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAAL 403

BLAST of CSPI05G30790 vs. NCBI nr
Match: XP_004141536.2 (mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KGN52650.1 hypothetical protein Csa_008142 [Cucumis sativus])

HSP 1 Score: 835.5 bits (2157), Expect = 2.0e-238
Identity = 407/409 (99.51%), Postives = 408/409 (99.76%), Query Frame = 0

Query: 1   MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP 60
           MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP
Sbjct: 8   MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP 67

Query: 61  NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 120
           NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG
Sbjct: 68  NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 127

Query: 121 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT 180
           IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT
Sbjct: 128 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT 187

Query: 181 MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY 240
           MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY
Sbjct: 188 MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY 247

Query: 241 GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA 300
           GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA
Sbjct: 248 GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA 307

Query: 301 LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGG 360
           LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSS RDRDAFLSKVFSIIYNLARNGATMAGG
Sbjct: 308 LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSVRDRDAFLSKVFSIIYNLARNGATMAGG 367

Query: 361 LVWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQHHL 410
           LVWQVMAEGMESYYDGYEIVLSQ+PSTTAIITHQSNNMAALNTRTQHHL
Sbjct: 368 LVWQVMAEGMESYYDGYEIVLSQTPSTTAIITHQSNNMAALNTRTQHHL 416

BLAST of CSPI05G30790 vs. NCBI nr
Match: XP_008459570.1 (PREDICTED: mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo] >TYK00481.1 mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 750.4 bits (1936), Expect = 8.4e-213
Identity = 367/409 (89.73%), Postives = 383/409 (93.64%), Query Frame = 0

Query: 1   MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP 60
           M+RWLIW L  LISTLS SSSSP  A FVGV+DGHFQLNGSPFLFNGFNSYWMMSVA DP
Sbjct: 7   MIRWLIWGLLLLISTLS-SSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAADP 66

Query: 61  NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 120
           NQR+KV+QVFRDAA AGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG
Sbjct: 67  NQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 126

Query: 121 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT 180
           IRMILSLVNNFKDYGGRAAYVRWAEAAGVQV +EDDFYTNQLI+TYYKNHVQKVL RKNT
Sbjct: 127 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKNT 186

Query: 181 MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY 240
           M+ L YM+D TIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSID+QHLVGIGMEGFY
Sbjct: 187 MSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGFY 246

Query: 241 GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA 300
           GDS P KIKANP S+K GTDFITNNLNKAID+ATIHAYPDAWLPGKSE TKMAFLEEW+A
Sbjct: 247 GDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWVA 306

Query: 301 LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGG 360
           LHWTDSKTILKKPLIFEEFG+SIRDQNQT  S RDRDAFLSKV+SIIYNLA+NGATMAGG
Sbjct: 307 LHWTDSKTILKKPLIFEEFGRSIRDQNQT-YSVRDRDAFLSKVYSIIYNLAKNGATMAGG 366

Query: 361 LVWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQHHL 410
           LVWQVMAEGMESYYDGYEIVLSQ+PST AIIT QSN MAAL TRTQHHL
Sbjct: 367 LVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALKTRTQHHL 413

BLAST of CSPI05G30790 vs. NCBI nr
Match: XP_004141631.3 (mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >XP_031740837.1 mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KAE8648995.1 hypothetical protein Csa_008431 [Cucumis sativus])

HSP 1 Score: 750.4 bits (1936), Expect = 8.4e-213
Identity = 366/409 (89.49%), Postives = 381/409 (93.15%), Query Frame = 0

Query: 1   MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP 60
           MMRW IW +FFLISTLS SSSSPAPAAFVGVQD HFQLNGSPFLFNGFNSYWMMSVA DP
Sbjct: 7   MMRWSIWGIFFLISTLS-SSSSPAPAAFVGVQDDHFQLNGSPFLFNGFNSYWMMSVAADP 66

Query: 61  NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 120
           NQR+KVTQVFRDAA AGLTVCRTWAFNDG  HALQISPGVYDESVFQ LDFVIFEARKYG
Sbjct: 67  NQRHKVTQVFRDAATAGLTVCRTWAFNDGALHALQISPGVYDESVFQALDFVIFEARKYG 126

Query: 121 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT 180
           IRM+LSLVNNF++YGGRAAYVRWAEAAGVQVH +DDFYTNQLI+TYYKN+V+KVL RKNT
Sbjct: 127 IRMLLSLVNNFEEYGGRAAYVRWAEAAGVQVHSDDDFYTNQLIRTYYKNYVKKVLRRKNT 186

Query: 181 MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY 240
           MNGL YMEDATIMGWELMNE RCQVDSSGNT+NRWVEEMGSYVKSIDKQHLVGIGMEGFY
Sbjct: 187 MNGLRYMEDATIMGWELMNEARCQVDSSGNTINRWVEEMGSYVKSIDKQHLVGIGMEGFY 246

Query: 241 GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA 300
           GDSSPNKIKANPGSFKFGTDF+TNNLNKAIDFATIH YPDAWLPGKSE T+MAFLEEW+A
Sbjct: 247 GDSSPNKIKANPGSFKFGTDFVTNNLNKAIDFATIHVYPDAWLPGKSEATRMAFLEEWMA 306

Query: 301 LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGG 360
           LHW DSK ILKKPLI EEFGKSIR QNQT  S RD DAFLSKV+SIIYNLAR GATMAGG
Sbjct: 307 LHWMDSKNILKKPLILEEFGKSIRGQNQT-FSVRDSDAFLSKVYSIIYNLARKGATMAGG 366

Query: 361 LVWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQHHL 410
           LVWQVMAEGMESYYDGYEIVLSQ+PST  IIT QSN MAALNTRTQHHL
Sbjct: 367 LVWQVMAEGMESYYDGYEIVLSQNPSTNTIITKQSNKMAALNTRTQHHL 413

BLAST of CSPI05G30790 vs. NCBI nr
Match: XP_038890634.1 (mannan endo-1,4-beta-mannosidase 1-like [Benincasa hispida])

HSP 1 Score: 707.2 bits (1824), Expect = 8.2e-200
Identity = 340/408 (83.33%), Postives = 373/408 (91.42%), Query Frame = 0

Query: 2   MRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDPN 61
           MRWLIW +FF++S LS SS SPAP AFVGVQDGHFQLNGSPF+FNGFNSYWMMSVA DPN
Sbjct: 7   MRWLIWEVFFILSGLS-SSPSPAPEAFVGVQDGHFQLNGSPFVFNGFNSYWMMSVAADPN 66

Query: 62  QRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYGI 121
           QR+KV+ VF DAA+A LTVCRTWAF DGG  ALQISPGVYDE VFQGLDFVI EA+KYGI
Sbjct: 67  QRHKVSNVFHDAASAALTVCRTWAFADGGSPALQISPGVYDEIVFQGLDFVICEAKKYGI 126

Query: 122 RMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNTM 181
           RMILSLVNN+KDYGGR  YVRWA+AAGV ++++DDFYTNQLIK+YYKNHVQ+VL+RKNT+
Sbjct: 127 RMILSLVNNYKDYGGREQYVRWAKAAGVDIYNDDDFYTNQLIKSYYKNHVQRVLTRKNTI 186

Query: 182 NGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFYG 241
           +GL YM+D TIMGWELMNEPRCQ+DSSGNT+NRWVEEMGSYVKSIDKQHLVGIGMEGF G
Sbjct: 187 SGLRYMDDPTIMGWELMNEPRCQLDSSGNTINRWVEEMGSYVKSIDKQHLVGIGMEGFCG 246

Query: 242 DSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIAL 301
           DSSP KIK+NP S++ GTDF+TNNLNK IDFATIHAYPDAWLPG+SE TKMAFLEEWI +
Sbjct: 247 DSSPTKIKSNPNSYRLGTDFLTNNLNKDIDFATIHAYPDAWLPGQSEATKMAFLEEWITI 306

Query: 302 HWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGGL 361
           HWTDSKTILKKPLIFEEFGKS R QNQT S+ RDRD+FLSKV+SIIYNLARNGATMAGGL
Sbjct: 307 HWTDSKTILKKPLIFEEFGKSTRGQNQT-SNVRDRDSFLSKVYSIIYNLARNGATMAGGL 366

Query: 362 VWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQHHL 410
           VWQVMAEGMESYYDGYEIVL Q+PST AIIT QSN MAALN +TQHHL
Sbjct: 367 VWQVMAEGMESYYDGYEIVLPQNPSTNAIITQQSNKMAALNIKTQHHL 412

BLAST of CSPI05G30790 vs. NCBI nr
Match: KAA0039297.1 (mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 673.7 bits (1737), Expect = 1.0e-189
Identity = 339/409 (82.89%), Postives = 355/409 (86.80%), Query Frame = 0

Query: 1   MMRWLIWALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDP 60
           M+RWLIW L  LISTLS SSSSP  A FVGV+DGHFQLNGSPFLFNGFNSYWMMSVA DP
Sbjct: 7   MIRWLIWGLLLLISTLS-SSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAADP 66

Query: 61  NQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYG 120
           NQR+KV+QVFRDAA AGLT                            GLDFVIFEARKYG
Sbjct: 67  NQRHKVSQVFRDAATAGLT----------------------------GLDFVIFEARKYG 126

Query: 121 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNT 180
           IRMILSLVNNFKDYGGRAAYVRWAEAAGVQV +EDDFYTNQLI+TYYKNHVQKVL RKNT
Sbjct: 127 IRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKNT 186

Query: 181 MNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFY 240
           M+ L YM+D TIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSID+QHLVGIGMEGFY
Sbjct: 187 MSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGFY 246

Query: 241 GDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIA 300
           GDS P KIKANP S+K GTDFITNNLNKAID+ATIHAYPDAWLPGKSE TKMAFLEEW+A
Sbjct: 247 GDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWVA 306

Query: 301 LHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGG 360
           LHWTDSKTILKKPLIFEEFG+SIRDQNQT  S RDRDAFLSKV+SIIYNLA+NGATMAGG
Sbjct: 307 LHWTDSKTILKKPLIFEEFGRSIRDQNQT-YSVRDRDAFLSKVYSIIYNLAKNGATMAGG 366

Query: 361 LVWQVMAEGMESYYDGYEIVLSQSPSTTAIITHQSNNMAALNTRTQHHL 410
           LVWQVMAEGMESYYDGYEIVLSQ+PST AIIT QSN MAAL TRTQHHL
Sbjct: 367 LVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALKTRTQHHL 385

BLAST of CSPI05G30790 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 405.2 bits (1040), Expect = 6.2e-113
Identity = 188/394 (47.72%), Postives = 261/394 (66.24%), Query Frame = 0

Query: 8   ALFFLISTLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDPNQRNKVT 67
           A+   +S     + +     FV  +   F LNG P+  NGFN+YW+M VA+DP+QR+K++
Sbjct: 11  AIVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKIS 70

Query: 68  QVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYGIRMILSL 127
             F+DA+  GLTV RTWAF+DGG+ ALQ SPG Y+E +FQGLDF + EAR++GI++ILS 
Sbjct: 71  TAFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSF 130

Query: 128 VNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNTMNGLIYM 187
            NN++ +GGR  YV WA + G  V  EDDF+T+ L+K +YKNH++ VL+R NT   + Y 
Sbjct: 131 ANNYESFGGRKQYVDWARSRGRPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYK 190

Query: 188 EDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFYGDSSPNK 247
           +D TIM WELMNEPRC  D SG  +  W+ EM ++VKS+D+ HL+  G+EGFYG SSP  
Sbjct: 191 DDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQS 250

Query: 248 IKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIALHWTDSK 307
              NP   +FGTDFI NN    IDF T+H+YPD W P  SE ++M FL +W+  H  D++
Sbjct: 251 KTLNPPG-QFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQ 310

Query: 308 TILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGGLVWQVMA 367
            +L KP+I  EFGKS++    T +    RD   + V+S IY  A+ G   AGGL WQ++ 
Sbjct: 311 NVLHKPIILAEFGKSMKKPGYTPA---QRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLV 370

Query: 368 EGMESYYDGYEIVLSQSPSTTAIITHQSNNMAAL 402
            G++++ DGY I+LSQS ST  +I+ QS  +  +
Sbjct: 371 NGIDNFQDGYGIILSQSSSTVNVISQQSRKLTLI 400

BLAST of CSPI05G30790 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 386.3 bits (991), Expect = 3.0e-107
Identity = 186/382 (48.69%), Postives = 245/382 (64.14%), Query Frame = 0

Query: 22  SPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAAAAGLTVC 81
           S +   FV  +   F LNG PF  NGFN+YW+   ATD   R K+T VF++A    LT+ 
Sbjct: 25  SASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEATDSTTRFKITYVFQNATIHDLTIV 84

Query: 82  RTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKYGIRMILSLVNNFKDYGGRAAYV 141
           RTW F DGG+ ALQI+PGVYDE  FQGLDF I EA++ GI+MI++ VNN+ D+GGR  YV
Sbjct: 85  RTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAKRLGIKMIITFVNNYSDFGGRKQYV 144

Query: 142 RWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNTMNGLIYMEDATIMGWELMNEP 201
            WA+  G  V  +DDFYTN L+K YYKNHV+ +++R NT   + Y ++ TIMGWELMNEP
Sbjct: 145 DWAKNTGQNVSSDDDFYTNPLVKQYYKNHVKTMVNRVNTFTKVEYKDEPTIMGWELMNEP 204

Query: 202 RCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFYGDSSP-NKIKANP-GSFKFGT 261
           +C+ D SG T+  W+ EM  YVKS+D +HL+  G+EGFYGDSSP  K   NP  +   GT
Sbjct: 205 QCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLEGFYGDSSPQRKTSLNPVAANVLGT 264

Query: 262 DFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIALHWTDSKTILKKPLIFEEF 321
           DFI N+   AIDFA+IH+YPD W P   E +++  L +W+  H  D++ ILKKPLI  EF
Sbjct: 265 DFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLLRKWLECHLEDAQNILKKPLILGEF 324

Query: 322 GKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEI 381
           GK       T +    RDA  +  F  IY  A  G   AG L W V+++GM ++ D   I
Sbjct: 325 GKPTNTPGYTQA---QRDAVFNATFDTIYESAEKGGPAAGALFWHVISDGMNNFKDPLSI 384

Query: 382 VLSQSPSTTAIITHQSNNMAAL 402
           VLS++ +T  IIT +S  +  +
Sbjct: 385 VLSENSTTVNIITEESRKLGLI 403

BLAST of CSPI05G30790 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 374.4 bits (960), Expect = 1.2e-103
Identity = 178/370 (48.11%), Postives = 244/370 (65.95%), Query Frame = 0

Query: 36  FQLNGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQ 95
           F LNG PF  NGFN+YWMM++A D + R KVT+VF+ A+A G+TV RTWAFNDG + ALQ
Sbjct: 55  FTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAFNDGQWRALQ 114

Query: 96  ISPGVYDESVFQGLDFVIFEARKYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDED 155
            SP VYDE VF+ LDFV+ EARKY IR+ILSLVNN+  YGG+A YV+W  A+G+ +  +D
Sbjct: 115 KSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNASGLNLTSDD 174

Query: 156 DFYTNQLIKTYYKNHVQKVLSRKNTMNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRW 215
           DF+TN  ++ +Y++HV+ VL+R NT   + Y  D TI  WELMNEPRC  D SG+ +  W
Sbjct: 175 DFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPSDPSGDKLQSW 234

Query: 216 VEEMGSYVKSIDKQHLVGIGMEGFYGDSSPNKIKANPGSF--KFGTDFITNNLNKAIDFA 275
           ++EM  +VKS+D +HLV IG+EGFYG S+P + + NP  +  + GTDFI NN    IDFA
Sbjct: 235 IQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIRNNQVLGIDFA 294

Query: 276 TIHAYPDAWLPGKSEGTKMAFLEEWIALHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSA 335
           ++H YPD+W+      + + F   W+  H  D++  L  P++F EFG S  D    +S  
Sbjct: 295 SVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSAHDPGFNTSF- 354

Query: 336 RDRDAFLSKVFSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQSPSTTAIITH 395
             RD  L+ V+ +  N  R G   AG LVWQV  +G E   DGY + L+++ + + II+ 
Sbjct: 355 --RDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHTASKIISL 414

Query: 396 QSNNMAALNT 404
           QS  +A  N+
Sbjct: 415 QSKRLAIFNS 421

BLAST of CSPI05G30790 vs. TAIR 10
Match: AT1G02310.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 362.1 bits (928), Expect = 6.0e-100
Identity = 189/408 (46.32%), Postives = 250/408 (61.27%), Query Frame = 0

Query: 1   MMRWLIWALFFLISTLSASSSSPA-PAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATD 60
           M+  L + LFFL   +  +    A    FVG     F LNG     NGFN+YWMM+ A D
Sbjct: 1   MLNILPFFLFFLPFLIGNNRICVAVKTGFVGRNGTQFVLNGEQVYLNGFNAYWMMTTAAD 60

Query: 61  PNQRNK--VTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEAR 120
              + +  VT   R A+A G+ V R W FN+G +  LQISPG Y E VF+GLDFV++EA 
Sbjct: 61  TASKGRATVTTALRQASAVGMNVARIWGFNEGDYIPLQISPGSYSEDVFKGLDFVVYEAG 120

Query: 121 KYGIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSR 180
           ++ I++I+SLVNNF+DYGGR  YV WA      + + D+FYTN  +K +YKNHV+ VL+R
Sbjct: 121 RFNIKLIISLVNNFEDYGGRKKYVEWA-----GLDEPDEFYTNSAVKQFYKNHVKTVLTR 180

Query: 181 KNTMNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGME 240
           KNT+ G +Y +D TI  WEL+NEPRC   ++ N +  WV+EM SYVKSID  HL+ IG+E
Sbjct: 181 KNTITGRMYKDDPTIFSWELINEPRCNDSTASNILQDWVKEMASYVKSIDSNHLLEIGLE 240

Query: 241 GFYGDSSPNKIKANPGS-FKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSE--GTKMAF 300
           GFYG+S P +   NPG     GTDFITNN    IDFATIH YPD+WLP +S   G +  F
Sbjct: 241 GFYGESIPERTVYNPGGRVLTGTDFITNNQIPDIDFATIHIYPDSWLPLQSSRTGEQDTF 300

Query: 301 LEEWIALHWTDSKTILKKPLIFEEFGKSIRDQNQTSSSARDRDAFLSKVFSIIYNLARNG 360
           ++ WI  H  D   I+KKPL+  EFGKS         S   R+ F  +V+ +IY+ AR G
Sbjct: 301 VDRWIGAHIEDCDNIIKKPLLITEFGKS---SKYPGFSLEKRNKFFQRVYDVIYDSARAG 360

Query: 361 ATMAGGLVWQVMAEGMESYYDGYEIVLSQSPSTTA-IITHQSNNMAAL 402
            +  GG+ WQ+         DGYE+ +   P+TTA +I  QS+ +  L
Sbjct: 361 GSCTGGVFWQLTTNRTGLLGDGYEVFMQAGPNTTAQLIADQSSKLKNL 400

BLAST of CSPI05G30790 vs. TAIR 10
Match: AT3G10900.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 352.8 bits (904), Expect = 3.6e-97
Identity = 177/382 (46.34%), Postives = 235/382 (61.52%), Query Frame = 0

Query: 28  FVGVQDGHFQLNGSPFLFNGFNSYWMMSVATDPNQRNKVTQVFRDAAAAGLTVCRTWAFN 87
           FV      F LNG PF  NGFN+YW+   ATDP  R K+T VF++A + GLT+ RTW F 
Sbjct: 29  FVSRNGVQFILNGKPFYANGFNAYWLAYEATDPTTRFKITNVFQNATSLGLTIARTWGFR 88

Query: 88  DGG-FHALQISPGVYDESVFQGLDFVIFEARKYGIRMILSLVNNFKDYGGRAAYVRWAEA 147
           DG  + ALQ +PG YDE  FQGLDFVI EA++ GI++I+ LVNN+ DYGG+  YV WA +
Sbjct: 89  DGAIYRALQTAPGSYDEQTFQGLDFVIAEAKRIGIKLIILLVNNWDDYGGKKQYVDWARS 148

Query: 148 AGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKNTMNGLIYMEDATIMGWELMNEPRCQVD 207
            G  V   DDFY N +IK +YKNHV+ VL+R NT   + Y ++  IM W+LMNEPRC VD
Sbjct: 149 KGEVVSSNDDFYRNPVIKDFYKNHVKTVLNRVNTFTKVAYKDEPAIMAWQLMNEPRCGVD 208

Query: 208 SSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGFYGDSSP-NKIKANPGSFK-FGTDFITN 267
            SG T+  W+ EM  +VKS+D  HL+  G EGFYGDSSP  K   NP S    G DFI N
Sbjct: 209 KSGKTLMDWINEMAPFVKSVDPNHLLSTGHEGFYGDSSPERKNSLNPVSANTVGADFIAN 268

Query: 268 NLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWIALHWTDSKTILKKPLIFEEFGKSIR 327
           +   AIDFA++H   D W     + +++AF++ W+  H  D++ ILKKP+I  EFG    
Sbjct: 269 HNIDAIDFASMHCGSDLWFQRLDQNSRLAFIKRWLEGHIEDAQNILKKPVILAEFGLGSD 328

Query: 328 DQNQTSSSARDRDAFLSKVFSIIYNLARNGATMAGGLVWQVMAEGMESYYDGYEIVLSQS 387
               T +   +RD   +  + IIY  A+ G + AG L W+V++EGM ++     I+LS  
Sbjct: 329 TPRYTLA---NRDGVFTTTYDIIYASAQKGGSAAGALFWEVISEGMSNFAGPSSIILSDK 388

Query: 388 PSTTAIITHQSNNMAALNTRTQ 407
            ST  II+  +  M  L   T+
Sbjct: 389 SSTVNIISEHARKMGLLGETTR 407

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZ032.4e-13860.96Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 ... [more]
Q6YM502.4e-13860.96Mannan endo-1,4-beta-mannosidase 5 OS=Solanum lycopersicum OX=4081 GN=MAN5 PE=2 ... [more]
O485403.9e-13658.15Mannan endo-1,4-beta-mannosidase 1 OS=Solanum lycopersicum OX=4081 GN=MAN1 PE=1 ... [more]
Q9FUQ66.2e-13457.88Mannan endo-1,4-beta-mannosidase 3 OS=Solanum lycopersicum OX=4081 GN=MAN3 PE=3 ... [more]
Q0JKM99.0e-11750.13Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Match NameE-valueIdentityDescription
A0A0A0KSS29.6e-23999.51Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G648710 PE=3... [more]
A0A5D3BKZ34.1e-21389.73Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CB044.1e-21389.73Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103498662 PE=3 SV... [more]
A0A5A7TCI24.8e-19082.89Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1CK631.6e-18577.67Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111012035 ... [more]
Match NameE-valueIdentityDescription
XP_004141536.22.0e-23899.51mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KGN52650.1 hypothetical pr... [more]
XP_008459570.18.4e-21389.73PREDICTED: mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo] >TYK00481.1 ma... [more]
XP_004141631.38.4e-21389.49mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >XP_031740837.1 mannan endo... [more]
XP_038890634.18.2e-20083.33mannan endo-1,4-beta-mannosidase 1-like [Benincasa hispida][more]
KAA0039297.11.0e-18982.89mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT5G66460.16.2e-11347.72Glycosyl hydrolase superfamily protein [more]
AT3G10890.13.0e-10748.69Glycosyl hydrolase superfamily protein [more]
AT5G01930.11.2e-10348.11Glycosyl hydrolase superfamily protein [more]
AT1G02310.16.0e-10046.32Glycosyl hydrolase superfamily protein [more]
AT3G10900.13.6e-9746.34Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 38..331
e-value: 4.3E-15
score: 55.9
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 25..402
e-value: 2.7E-125
score: 420.2
NoneNo IPR availablePANTHERPTHR31451:SF53MANNAN ENDO-1,4-BETA-MANNOSIDASE-LIKE PROTEINcoord: 2..407
IPR045053Mannan endo-1,4-beta-mannosidase-likePANTHERPTHR31451FAMILY NOT NAMEDcoord: 2..407
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 28..393

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G30790.1CSPI05G30790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds