CSPI05G30220 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G30220
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Peptide chain release factor 1
Location	Chr5: 28246505 .. 28249377 (+)
RNA-Seq Expression	CSPI05G30220
Synteny	CSPI05G30220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCAAATTGGTTTTGTGAACTGAGAAAATGGCAAACGCAAATATGCTATGGAGTTCCGCTAAGATTCTTGGATTTCCTTCTCCGATAGCAAGCAACGTCCGATGGGCAAGGTTCGATTATACATTTCGGCTTCGGCATCGTCCATTCGAGGGCTCTTTCTTCTTCTTCAACTCCTTGCGTTTCAATTGCAGCTCCAGTAATGCAAATTCAGTATGTAATGGCAAACAGTACTTAGTTTTGTCCGACGAAGAGCTGATGAAACAGTGCGAAATGGGCACGTTCAAGGCGTCGGGTCCCGGCGGTCAGCACCGCAACAAGCGCGAGTCTGCTGTTCGTTTGAAGCACATTCCCACTGGTATCATTGCTCAGGTTCATATTTGTTTCATACATACAATCAATTTCATTGATATACTTCTGTTTCTTTTGCTCTGAAGTTAAATTCTTTGGCCCTTTATTGCCAAGGCTGTTGAGGATCGATCCCAGCATAAGAATCGATCCAGTGCCTTAGCTCGTCTTCGTGCTCTCTTGGCCCTAAAAGGTGCGATGGATTATTTATTAGATGTTTGTTTGTTTGTTTGTTTGTTTTTTATCTCATTTCTCATTTCATGTGTTGATGTATTGAATTCAGTTAGGAACCCTGTGGATCTTGAAGATTATTCGCCTCCCCCAGAGCTTCTTCAGATTCTTCCTCTTAAGTCCACTGTTAGGCCACCGGAATGTGGCCCTCAAATTGGTCCTAACAATTCTAAGTTTCTGCCGGTATCTCTCTTTTCTAGTTCTTTCTATATGTCTATATGTGCATATGAGTGTGCGTTTATGATTTGATTTTCTTTAAGATACATAAAATTCTTTTTTGAAATGAAAACAAGATTTATTGAAATAATGAATGAGTCTAATGCTCAAACTACATGAGATGAAACAAAAATAAAAACTGCAAACTTGCCAAACCAAACCATAATGTAAAACATAGACTAAACTTCTGATGAAGCAGTAAAAACATTCGAGTTTTAAACAAGTAACTTGAGTTGAACAATCAGCAAAGTGTTTAAATAGAATATATATTTACACACACACACACACACACACACGAGAAGATTATGAATGATAGCTGAATTGCTTAATTTCCAGGGCATGCAAGCTCTACTGGATCTAATTTCCACACTTGATGGTTCTGTATCAGACACAGCTAAGCTTTTAGGGTATGTATGCTATTCATGTGTTATCCTTTGTTTCTTTTTACTTTGTTTCCAACCTGAGCTAGCATTCACAGTTAAATCCAGAGAATGGGAGTGTTTCAATTATGTGAGAATATTGGGCTTTTATCAAGAAATTGAATCTGCTGATGTGTGGATGTTATAATGGTTGAGATATGGAGATTAGTGGAAGGGGTATCATTGGTAGGTTTATGGTTTTTGGGGAATGAAGAAGATTGGCAATTTTTTCGAGGGCGTGAGGGAAGTGACTAATGAGTTTGGACCTCTGGAAGATGTTGGAGGATAATTTTCTCGGGAAGTGTCTAGTTGAAGGCAGAAGCCTTGCCTTGTGTGTCTTGAAGAAGGCTTCTTTCATGGGATCACTATGAGTAGGAGGAAGGAGCTTTTACATTTCTTTTATTACTTTGGACGTTGGTATCGCTTTCATTAAGAAGAGTAGACTTAAGGATTTGATCGGTCACCTACTAAGGAAGCAGACCAAATTTGGTTAGAGAGAGCTTCTTAGTTTGATGACTAGCTCTGCAGAAGGACATTCCTTTTGATCTCCATTCCTTCTCCCATCTTGTCTGTTGCGAGGTGGGGGAAGGCATGAGAGAAGCTACAGAAGCTGTCTCTCTCTTTGTCTCTCTTCTCATTAGTTAGGGAGTTCAATTTTTGAAGAAAAGGTGTTCATTAAAATATTAGTCTATTCTTTTCCATTATGTCTCAAAATGAGATAACCTTAAATATTGAGAGGTGGATTAATTGATGCAATCAGAACTCTTGAATTACAATTTAGATCAATCGTCCAAAAACGATTCTTCACCTTTCCCATTTTTAATATGTCAGTGATATATATTAGTTGCAAACTCTAATTGTTATTTTAACGTTTAAAAATATAGGTTAACAACCGGTGCTCTTTCAAGGTTAATCCTATCCGATGATGCTCTTAGAATGGCTGTAAATGATATCAGGATGTCCAAGGTAACACCATACTATTTATGAGATCATGATAGTTACCATTTATTATCTTTTTTCAAAAGATTTTATATGCACCACTCACATACTAATTTTTCAACATAAGTCAATTGACACCCATCGATCGTGTCTTCCAAAATTTGAAATTTACAGGGTCTGAAGCCGCTCAAGTAGATTGTCAAACTGAGCATAACTTCAGCAGCAATTGACATGTATTACTTCTTTCGAGATCCCAAGTTTAAAACTTCATAACCATTAAGTAAAAAGTGGGAAGCTCAATGAACTTGTAACTAGAGAGACACTACGCAAGCAACTATCTTTCTTATTATCATGCTTAGCTCAACCGCTCAGAGCATTACTCACATCTTTCTTGATGATCGGAGGTTTGTATCTCCAATCTCGTTGTTAATGCATGAAGGTTGAAAACACTTTCGCTTGTTACTCTTCCCTATTACTGCAGATTTTAAACTAGAAACGCTTCGATTTGAATCTGCTTTATCGACAAAGAGTTCTACTTTGTTCGCTCAACTGTTGTCAATTCTCTTGTATATGTCTTGTTCTGGAAAGAGGAAAAAGATTTACTTTTACTATAGCCTATATGTAAGAGGTTTATGTTGTTGTATCTTAATCTTGGAAAAATATTGTAATGTTTATCTTAAATATTAAAATTGAATTATGCATTGTAATGTTCAGTTTCTAATA

mRNA sequence

TCCAAATTGGTTTTGTGAACTGAGAAAATGGCAAACGCAAATATGCTATGGAGTTCCGCTAAGATTCTTGGATTTCCTTCTCCGATAGCAAGCAACGTCCGATGGGCAAGGTTCGATTATACATTTCGGCTTCGGCATCGTCCATTCGAGGGCTCTTTCTTCTTCTTCAACTCCTTGCGTTTCAATTGCAGCTCCAGTAATGCAAATTCAGTATGTAATGGCAAACAGTACTTAGTTTTGTCCGACGAAGAGCTGATGAAACAGTGCGAAATGGGCACGTTCAAGGCGTCGGGTCCCGGCGGTCAGCACCGCAACAAGCGCGAGTCTGCTGTTCGTTTGAAGCACATTCCCACTGGTATCATTGCTCAGGCTGTTGAGGATCGATCCCAGCATAAGAATCGATCCAGTGCCTTAGCTCGTCTTCGTGCTCTCTTGGCCCTAAAAGTTAGGAACCCTGTGGATCTTGAAGATTATTCGCCTCCCCCAGAGCTTCTTCAGATTCTTCCTCTTAAGTCCACTGTTAGGCCACCGGAATGTGGCCCTCAAATTGGTCCTAACAATTCTAAGTTTCTGCCGGGCATGCAAGCTCTACTGGATCTAATTTCCACACTTGATGGTTCTGTATCAGACACAGCTAAGCTTTTAGGGTTAACAACCGGTGCTCTTTCAAGGTTAATCCTATCCGATGATGCTCTTAGAATGGCTGTAAATGATATCAGGATGTCCAAGGGTCTGAAGCCGCTCAAGTAGATTGTCAAACTGAGCATAACTTCAGCAGCAATTGACATGTATTACTTCTTTCGAGATCCCAAGTTTAAAACTTCATAACCATTAAGTAAAAAGTGGGAAGCTCAATGAACTTGTAACTAGAGAGACACTACGCAAGCAACTATCTTTCTTATTATCATGCTTAGCTCAACCGCTCAGAGCATTACTCACATCTTTCTTGATGATCGGAGGTTTGTATCTCCAATCTCGTTGTTAATGCATGAAGGTTGAAAACACTTTCGCTTGTTACTCTTCCCTATTACTGCAGATTTTAAACTAGAAACGCTTCGATTTGAATCTGCTTTATCGACAAAGAGTTCTACTTTGTTCGCTCAACTGTTGTCAATTCTCTTGTATATGTCTTGTTCTGGAAAGAGGAAAAAGATTTACTTTTACTATAGCCTATATGTAAGAGGTTTATGTTGTTGTATCTTAATCTTGGAAAAATATTGTAATGTTTATCTTAAATATTAAAATTGAATTATGCATTGTAATGTTCAGTTTCTAATA

Coding sequence (CDS)

ATGGCAAACGCAAATATGCTATGGAGTTCCGCTAAGATTCTTGGATTTCCTTCTCCGATAGCAAGCAACGTCCGATGGGCAAGGTTCGATTATACATTTCGGCTTCGGCATCGTCCATTCGAGGGCTCTTTCTTCTTCTTCAACTCCTTGCGTTTCAATTGCAGCTCCAGTAATGCAAATTCAGTATGTAATGGCAAACAGTACTTAGTTTTGTCCGACGAAGAGCTGATGAAACAGTGCGAAATGGGCACGTTCAAGGCGTCGGGTCCCGGCGGTCAGCACCGCAACAAGCGCGAGTCTGCTGTTCGTTTGAAGCACATTCCCACTGGTATCATTGCTCAGGCTGTTGAGGATCGATCCCAGCATAAGAATCGATCCAGTGCCTTAGCTCGTCTTCGTGCTCTCTTGGCCCTAAAAGTTAGGAACCCTGTGGATCTTGAAGATTATTCGCCTCCCCCAGAGCTTCTTCAGATTCTTCCTCTTAAGTCCACTGTTAGGCCACCGGAATGTGGCCCTCAAATTGGTCCTAACAATTCTAAGTTTCTGCCGGGCATGCAAGCTCTACTGGATCTAATTTCCACACTTGATGGTTCTGTATCAGACACAGCTAAGCTTTTAGGGTTAACAACCGGTGCTCTTTCAAGGTTAATCCTATCCGATGATGCTCTTAGAATGGCTGTAAATGATATCAGGATGTCCAAGGGTCTGAAGCCGCTCAAGTAG

Protein sequence

MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNANSVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSKFLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK*

Homology

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BLAST of CSPI05G30220 vs. ExPASy Swiss-Prot
Match: A7ZE19 (Peptide chain release factor 2 OS=Campylobacter concisus (strain 13826) OX=360104 GN=prfB PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.5e-12
Identity = 33/59 (55.93%), Postives = 45/59 (76.27%), Query Frame = 0


Query: 78  KQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHKNRSSALARLRALL 137
           K  ++ T++ASG GGQH NK ESA+R+ HIPTGI+ Q   DRSQHKNR++A+  L++ L
Sbjct: 236 KDLKIDTYRASGAGGQHVNKTESAIRITHIPTGIVVQCQNDRSQHKNRATAMKMLKSRL 294

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BLAST of CSPI05G30220 vs. ExPASy Swiss-Prot
Match: B1XYX1 (Peptide chain release factor 1 OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) OX=395495 GN=prfA PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.9e-12
Identity = 35/78 (44.87%), Postives = 53/78 (67.95%), Query Frame = 0


Query: 79  QCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHKNRSSALARLRALLAL 138
           +  + T++ASG GGQH NK +SAVR+ H+PTG++A+  +DRSQH+N++ ALA L A L  
Sbjct: 221 ELRIDTYRASGAGGQHINKTDSAVRVTHLPTGLVAECQDDRSQHRNKARALAVLAARLRD 280

Query: 139 KVRNPVDLEDYSPPPELL 157
           +VR     +D +    L+
Sbjct: 281 RVRQEQAAKDAAARKSLI 298

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BLAST of CSPI05G30220 vs. ExPASy Swiss-Prot
Match: A5WFG1 (Peptide chain release factor 1 OS=Psychrobacter sp. (strain PRwf-1) OX=349106 GN=prfA PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.9e-12
Identity = 36/68 (52.94%), Postives = 48/68 (70.59%), Query Frame = 0


Query: 82  MGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHKNRSSALARLRA-LLALKV 141
           M TF++SG GGQH N  +SAVRL HIPTG++A+  ++RSQHKNR+ A+  L A +   KV
Sbjct: 227 MDTFRSSGAGGQHVNTTDSAVRLTHIPTGVVAECQQERSQHKNRAKAMQMLIARIQQAKV 286

Query: 142 RNPVDLED 149
           +  VD  D
Sbjct: 287 QEQVDTAD 294

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BLAST of CSPI05G30220 vs. ExPASy Swiss-Prot
Match: A8EWG5 (Peptide chain release factor 2 OS=Arcobacter butzleri (strain RM4018) OX=367737 GN=prfB PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.0e-11
Identity = 33/59 (55.93%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 78  KQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHKNRSSALARLRALL 137
           K   + T++ASG GGQH NK ESA+R+ HIPTGI+ Q   DRSQHKN+ SA   L++ L
Sbjct: 236 KDIRIDTYRASGAGGQHVNKTESAIRITHIPTGIVVQCQNDRSQHKNKDSAFKMLKSRL 294

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BLAST of CSPI05G30220 vs. ExPASy Swiss-Prot
Match: A6Q582 (Peptide chain release factor 2 OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=prfB PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.0e-11
Identity = 32/59 (54.24%), Postives = 44/59 (74.58%), Query Frame = 0


Query: 78  KQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHKNRSSALARLRALL 137
           K   + T++ASG GGQH NK +SA+R+ HIPTGI+ Q   DRSQHKNR++A+  L++ L
Sbjct: 236 KDIRVDTYRASGAGGQHVNKTDSAIRITHIPTGIVVQCQNDRSQHKNRATAMKMLKSRL 294

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BLAST of CSPI05G30220 vs. ExPASy TrEMBL
Match: A0A0A0KUM8 (RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G646680 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 8.7e-131
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60
           MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN
Sbjct: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120
           SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS
Sbjct: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180
           QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240
           FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240

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BLAST of CSPI05G30220 vs. ExPASy TrEMBL
Match: A0A5A7T8S5 (Peptide chain release factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001580 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.4e-125
Identity = 230/240 (95.83%), Postives = 235/240 (97.92%), Query Frame = 0


Query: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60
           MANANMLW+SAKILGFP+PIASNVRWARF Y FR RH PFEGSFFFFNSLRFNCSSSNAN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWARFGYKFRFRHHPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120
           SVC+GK+YLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180
           QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILP KSTVRPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240
           FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKG+KPLK
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGMKPLK 240

[Close]

BLAST of CSPI05G30220 vs. ExPASy TrEMBL
Match: A0A1S3CAA2 (uncharacterized protein LOC103498609 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498609 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.4e-125
Identity = 230/240 (95.83%), Postives = 235/240 (97.92%), Query Frame = 0


Query: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60
           MANANMLW+SAKILGFP+PIASNVRWARF Y FR RH PFEGSFFFFNSLRFNCSSSNAN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWARFGYKFRFRHHPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120
           SVC+GK+YLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180
           QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILP KSTVRPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240
           FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKG+KPLK
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGMKPLK 240

[Close]

BLAST of CSPI05G30220 vs. ExPASy TrEMBL
Match: A0A1S3CAT7 (uncharacterized protein LOC103498609 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498609 PE=4 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.5e-106
Identity = 207/240 (86.25%), Postives = 212/240 (88.33%), Query Frame = 0


Query: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60
           MANANMLW+SAKILGFP+PIASNVRWA                           SSSNAN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWA---------------------------SSSNAN 60

Query: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120
           SVC+GK+YLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180
           QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILP KSTVRPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240
           FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKG+KPLK
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGMKPLK 213

[Close]

BLAST of CSPI05G30220 vs. ExPASy TrEMBL
Match: A0A6J1EX22 (uncharacterized protein LOC111438876 OS=Cucurbita moschata OX=3662 GN=LOC111438876 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 8.2e-105
Identity = 201/240 (83.75%), Postives = 214/240 (89.17%), Query Frame = 0


Query: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60
           MA ANMLW SAKIL  P PIASN RW R   TFR+    FEGS FF NSLR  CSSS+AN
Sbjct: 1   MATANMLWKSAKILSIPVPIASNGRWGRIGCTFRIHQ--FEGS-FFSNSLRSTCSSSSAN 60

Query: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120
           SV  GK+YL+LSDEELM+QCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVWKGKEYLILSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120

Query: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180
           QHKNR+SALARLRA LALKVRNPVDLEDYSPPPELLQILPLKST+RPP CGPQIGPNNSK
Sbjct: 121 QHKNRASALARLRAQLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPGCGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240
           FLPGMQALLDLI TLDGS+SDTAK +GLTTGA+SRLILSDD+LRMAVNDIR+SKG+KPLK
Sbjct: 181 FLPGMQALLDLIYTLDGSISDTAKYIGLTTGAVSRLILSDDSLRMAVNDIRISKGMKPLK 237

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BLAST of CSPI05G30220 vs. NCBI nr
Match: XP_004141513.1 (uncharacterized protein LOC101217430 isoform X1 [Cucumis sativus] >KGN52594.1 hypothetical protein Csa_007986 [Cucumis sativus])

HSP 1 Score: 476.1 bits (1224), Expect = 1.8e-130
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60
           MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN
Sbjct: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120
           SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS
Sbjct: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180
           QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240
           FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240

[Close]

BLAST of CSPI05G30220 vs. NCBI nr
Match: XP_008459491.1 (PREDICTED: uncharacterized protein LOC103498609 isoform X1 [Cucumis melo] >KAA0039363.1 peptide chain release factor 1 [Cucumis melo var. makuwa] >TYK00546.1 peptide chain release factor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 458.8 bits (1179), Expect = 3.0e-125
Identity = 230/240 (95.83%), Postives = 235/240 (97.92%), Query Frame = 0


Query: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60
           MANANMLW+SAKILGFP+PIASNVRWARF Y FR RH PFEGSFFFFNSLRFNCSSSNAN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWARFGYKFRFRHHPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120
           SVC+GK+YLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180
           QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILP KSTVRPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240
           FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKG+KPLK
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGMKPLK 240

[Close]

BLAST of CSPI05G30220 vs. NCBI nr
Match: XP_038891024.1 (peptide chain release factor 1 [Benincasa hispida])

HSP 1 Score: 410.2 bits (1053), Expect = 1.2e-110
Identity = 214/240 (89.17%), Postives = 221/240 (92.08%), Query Frame = 0


Query: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60
           MANANML +SAKILGFP PIAS  RWARF  TF   H PFEGS FFFNSL  NCSS+ AN
Sbjct: 1   MANANMLCNSAKILGFPLPIASKGRWARFGCTF--WHHPFEGS-FFFNSLHSNCSSTTAN 60

Query: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120
           SV NGK+YLVLSDEELMKQCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVWNGKEYLVLSDEELMKQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120

Query: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180
           QHKNR+SALARLRA LALKVRNPVDLEDYSPPPELLQILPLKST+RPPECGPQIGP NSK
Sbjct: 121 QHKNRASALARLRAFLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPKNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240
           FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIR+SKG+KPLK
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRISKGMKPLK 237

[Close]

BLAST of CSPI05G30220 vs. NCBI nr
Match: XP_031740950.1 (uncharacterized protein LOC101217430 isoform X2 [Cucumis sativus])

HSP 1 Score: 404.4 bits (1038), Expect = 6.7e-109
Identity = 213/240 (88.75%), Postives = 213/240 (88.75%), Query Frame = 0


Query: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60
           MANANMLWSSAKILGFPSPIASNVRWA                           SSSNAN
Sbjct: 1   MANANMLWSSAKILGFPSPIASNVRWA---------------------------SSSNAN 60

Query: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120
           SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS
Sbjct: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180
           QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240
           FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 213

[Close]

BLAST of CSPI05G30220 vs. NCBI nr
Match: XP_008459492.1 (PREDICTED: uncharacterized protein LOC103498609 isoform X2 [Cucumis melo])

HSP 1 Score: 395.6 bits (1015), Expect = 3.1e-106
Identity = 207/240 (86.25%), Postives = 212/240 (88.33%), Query Frame = 0


Query: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60
           MANANMLW+SAKILGFP+PIASNVRWA                           SSSNAN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWA---------------------------SSSNAN 60

Query: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120
           SVC+GK+YLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180
           QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILP KSTVRPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 240
           FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKG+KPLK
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGMKPLK 213

[Close]

BLAST of CSPI05G30220 vs. TAIR 10
Match: AT1G33330.1 (Class I peptide chain release factor )

HSP 1 Score: 260.0 bits (663), Expect = 1.9e-69
Identity = 130/173 (75.14%), Postives = 150/173 (86.71%), Query Frame = 0


Query: 68  YLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHKNRSS 127
           YL  +DEELMKQC + TF+ SGPGGQHRNKR+SAVRLKH+PTGI+AQAVEDRSQHKNR+S
Sbjct: 86  YLKFTDEELMKQCRLETFRVSGPGGQHRNKRDSAVRLKHLPTGIVAQAVEDRSQHKNRAS 145

Query: 128 ALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSKFLPGMQA 187
           AL RLR LLA+KVRN VD+E Y+PPPELLQILP KST+R    G QIGPNN KF+PGMQA
Sbjct: 146 ALNRLRTLLAIKVRNKVDIEAYAPPPELLQILPPKSTIR-TSSGSQIGPNNPKFVPGMQA 205

Query: 188 LLDLISTLDGSVSDTAKLLGLTTGALSRLILSDDALRMAVNDIRMSKGLKPLK 241
           LLD+IS  DGS++D+AKLLGL+TG LSRLILS D LRMAVN +R +KG+KPLK
Sbjct: 206 LLDVISASDGSIADSAKLLGLSTGGLSRLILSHDGLRMAVNSMRAAKGIKPLK 257

[Close]

BLAST of CSPI05G30220 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 61.6 bits (148), Expect = 9.9e-10
Identity = 31/73 (42.47%), Postives = 47/73 (64.38%), Query Frame = 0


Query: 64  NGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHK 123
           +G   + ++D +L     +  F++ G GGQH N  +SAVR+ HIPTGI A    +RSQH 
Sbjct: 336 DGSTRVEINDSDL----RIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHS 395

Query: 124 NRSSALARLRALL 137
           N++SA+A L++ L
Sbjct: 396 NKASAMAVLQSRL 404

[Close]

BLAST of CSPI05G30220 vs. TAIR 10
Match: AT2G47020.1 (Peptide chain release factor 1 )

HSP 1 Score: 56.6 bits (135), Expect = 3.2e-08
Identity = 27/63 (42.86%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 84  TFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHKNRSSAL----ARLRALLALK 143
           T+++ G GGQH N   SAVR+ H+PTG++    ++RSQH NR+ AL    ARL  +  L+
Sbjct: 278 TYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQHMNRAKALKVLCARLYEIERLR 337

[Close]

BLAST of CSPI05G30220 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 56.2 bits (134), Expect = 4.1e-08
Identity = 27/51 (52.94%), Postives = 37/51 (72.55%), Query Frame = 0


Query: 86  KASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHKNRSSALARLRALL 137
           +A G GGQ+ NK E+AVR+ HIPTG+  +  E+RSQ  N++ AL RL+A L
Sbjct: 325 RAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKL 375

[Close]

BLAST of CSPI05G30220 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 56.2 bits (134), Expect = 4.1e-08
Identity = 27/51 (52.94%), Postives = 37/51 (72.55%), Query Frame = 0


Query: 86  KASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRSQHKNRSSALARLRALL 137
           +A G GGQ+ NK E+AVR+ HIPTG+  +  E+RSQ  N++ AL RL+A L
Sbjct: 324 RAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKL 374

The following BLAST results are available for this feature:

BLAST of CSPI05G30220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7ZE19	3.5e-12	55.93	Peptide chain release factor 2 OS=Campylobacter concisus (strain 13826) OX=36010...	[more]
B1XYX1	7.9e-12	44.87	Peptide chain release factor 1 OS=Leptothrix cholodnii (strain ATCC 51168 / LMG ...	[more]
A5WFG1	7.9e-12	52.94	Peptide chain release factor 1 OS=Psychrobacter sp. (strain PRwf-1) OX=349106 GN...	[more]
A8EWG5	1.0e-11	55.93	Peptide chain release factor 2 OS=Arcobacter butzleri (strain RM4018) OX=367737 ...	[more]
A6Q582	1.0e-11	54.24	Peptide chain release factor 2 OS=Nitratiruptor sp. (strain SB155-2) OX=387092 G...	[more]

BLAST of CSPI05G30220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KUM8	8.7e-131	100.00	RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G646680 P...	[more]
A0A5A7T8S5	1.4e-125	95.83	Peptide chain release factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3CAA2	1.4e-125	95.83	uncharacterized protein LOC103498609 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S3CAT7	1.5e-106	86.25	uncharacterized protein LOC103498609 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1EX22	8.2e-105	83.75	uncharacterized protein LOC111438876 OS=Cucurbita moschata OX=3662 GN=LOC1114388...	[more]

BLAST of CSPI05G30220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141513.1	1.8e-130	100.00	uncharacterized protein LOC101217430 isoform X1 [Cucumis sativus] >KGN52594.1 hy...	[more]
XP_008459491.1	3.0e-125	95.83	PREDICTED: uncharacterized protein LOC103498609 isoform X1 [Cucumis melo] >KAA00...	[more]
XP_038891024.1	1.2e-110	89.17	peptide chain release factor 1 [Benincasa hispida]	[more]
XP_031740950.1	6.7e-109	88.75	uncharacterized protein LOC101217430 isoform X2 [Cucumis sativus]	[more]
XP_008459492.1	3.1e-106	86.25	PREDICTED: uncharacterized protein LOC103498609 isoform X2 [Cucumis melo]	[more]

BLAST of CSPI05G30220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G33330.1	1.9e-69	75.14	Class I peptide chain release factor	[more]
AT1G56350.1	9.9e-10	42.47	Peptide chain release factor 2	[more]
AT2G47020.1	3.2e-08	42.86	Peptide chain release factor 1	[more]
AT5G36170.1	4.1e-08	52.94	high chlorophyll fluorescent 109	[more]
AT5G36170.2	4.1e-08	52.94	high chlorophyll fluorescent 109	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.160.20		coord: 48..143 e-value: 5.9E-25 score: 89.1
None	No IPR available	PANTHER	PTHR43804:SF6	CLASS I PEPTIDE CHAIN RELEASE FACTOR	coord: 46..237
None	No IPR available	PANTHER	PTHR43804	LD18447P	coord: 46..237
None	No IPR available	SUPERFAMILY	75620	Release factor	coord: 78..138
IPR000352	Peptide chain release factor class I	PFAM	PF00472	RF-1	coord: 78..137 e-value: 1.8E-19 score: 69.8

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G30220.1	CSPI05G30220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006415	translational termination
molecular_function	GO:0003747	translation release factor activity