CSPI05G28560 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G28560
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	metalloendoproteinase 2-MMP-like
Location	Chr5: 26913603 .. 26915240 (-)
RNA-Seq Expression	CSPI05G28560
Synteny	CSPI05G28560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATTTCAAGGGCTATCATTATTCTTCACCTTCACTCTTTTTCTTATAATTTCCCTCTTCCCTTTAATCTCATCTTCTCAGCCAAAACCTGTGGATGGATTGGTGTTTCTAAAAAACCTTAATGGATGCAAGAAAGGGGATAAAGTGGAAGGAATTCATCAAATGAAGAAATACCTTCAACATTTTGGGTACTTGAATAATGATGTTCATATTGATTCTGAATCTAGTGATGATGAGTTTGATGAGATCCTGGAGTTCGCTGTCAAAACTTACCAAATCAACTACAATCTTAAGGTCACGGGAACTCTCGACAACTCGACGCTAATGCAAATGTCGAAACCTCGATGTGGGGTTGCGGATATTATCAACGGAAAGACGAGTATGAAATCAGGCAAGAGATTGGTGAACCAACATAGAAAAATAAGTGGTCATTTCCATGAAGTTTCCCACTTTGCTTTCTTTGAAGGAAACCTTAGATGGCCAGATTCCAAATCTCACCTCACTTACGGTAAATTTAATGTTCTATTCATATTCTTTTCATCACCAAATTTTTTTTTCGAATTTTAAGGATTTTCATACTCTATTAGAAAAATATAGGATTTTATCTAAAAAACAATATCAACTTCTAAGAGAAGAAATAATTATGTAGTTCATTAAGATTCATGGAGAAAAAAAATTATTTTTTTTAAAATAAGAAAATATCATGAAAATAAAGAGAATATATTATTTTATTTTTAAATGAAAAAATGAAAAAATGAAAGACATGGTTACTAGAATTAATAAAGATACATCACAATCTTTTTATTTTATTTTCAAATAGAAGTTATGGGTAAATTAGATGGATAAATAAAAGAGTATGAACGGGAGGCAAGAAGAAACATCTTCCAACCGAGAGACTTCCTCATCTTTCTAAAATGATATATTAGATTACTCATCTTATTATCAATTATCTATTTATAATACAAATTATTATAAATATTGTAGATTTTTTCGTCTATTTTTATAATGTGATAGTATCGTTGAATTGAAAATTTTAAACTAATGAGTTGTCGTAAATGTGAATTAGGATTCCTCCCAGGAACTCCGTCAGAGGCGATAAGTCCAGTGAATCGAGCATTTAGGACATGGGCTGCAAACACTCACTTCAGTTTCTCCCAAGAATCAAAGTATGAAAATGCAGACATCAAAATTAGTTTTGAAAAAGGTGACCATGGAGATGGCTTTCCCTTTGACAGTGTTGGAGGGGTGTTAGCCCATGCTTTTGCCCCCACAGATGGGCGATTACATTTCGATGCAGTCGAGCATTGGGCCGATGGTGCTGTTCCTAAGTCTTACGATATGGAGACCGTGGCGCTACATGAGATCGGACACCTTCTCGGCCTTCATCACAGCTCGGTGGAAGGAGCCATTATGTGGCCATCCATCATGGGAGGAGCTACCAAAGGTTTACATGCTGATGATATTGAAGGCATTAAGGTTTTATATACTACTTCTTGAGAAGTAGTATTGATTTTAACTTTGTATTACACTTCTAAACTATATAACCAATAATTATTTTTTCCTCACACTTATAATAATCCACACACACATATATAGGACATTTCATTTGTTGTAAGGAGAGATGTGAAGCTTTCC

mRNA sequence

ATGGGATTTCAAGGGCTATCATTATTCTTCACCTTCACTCTTTTTCTTATAATTTCCCTCTTCCCTTTAATCTCATCTTCTCAGCCAAAACCTGTGGATGGATTGGTGTTTCTAAAAAACCTTAATGGATGCAAGAAAGGGGATAAAGTGGAAGGAATTCATCAAATGAAGAAATACCTTCAACATTTTGGGTACTTGAATAATGATGTTCATATTGATTCTGAATCTAGTGATGATGAGTTTGATGAGATCCTGGAGTTCGCTGTCAAAACTTACCAAATCAACTACAATCTTAAGGTCACGGGAACTCTCGACAACTCGACGCTAATGCAAATGTCGAAACCTCGATGTGGGGTTGCGGATATTATCAACGGAAAGACGAGTATGAAATCAGGCAAGAGATTGGTGAACCAACATAGAAAAATAAGTGGTCATTTCCATGAAGTTTCCCACTTTGCTTTCTTTGAAGGAAACCTTAGATGGCCAGATTCCAAATCTCACCTCACTTACGGATTCCTCCCAGGAACTCCGTCAGAGGCGATAAGTCCAGTGAATCGAGCATTTAGGACATGGGCTGCAAACACTCACTTCAGTTTCTCCCAAGAATCAAAGTATGAAAATGCAGACATCAAAATTAGTTTTGAAAAAGGTGACCATGGAGATGGCTTTCCCTTTGACAGTGTTGGAGGGGTGTTAGCCCATGCTTTTGCCCCCACAGATGGGCGATTACATTTCGATGCAGTCGAGCATTGGGCCGATGGTGCTGTTCCTAAGTCTTACGATATGGAGACCGTGGCGCTACATGAGATCGGACACCTTCTCGGCCTTCATCACAGCTCGGTGGAAGGAGCCATTATGTGGCCATCCATCATGGGAGGAGCTACCAAAGGTTTACATGCTGATGATATTGAAGGCATTAAGGTTTTATATACTACTTCTTGAGAAGTAGTATTGATTTTAACTTTGTATTACACTTCTAAACTATATAACCAATAATTATTTTTTCCTCACACTTATAATAATCCACACACACATATATAGGACATTTCATTTGTTGTAAGGAGAGATGTGAAGCTTTCC

Coding sequence (CDS)

ATGGGATTTCAAGGGCTATCATTATTCTTCACCTTCACTCTTTTTCTTATAATTTCCCTCTTCCCTTTAATCTCATCTTCTCAGCCAAAACCTGTGGATGGATTGGTGTTTCTAAAAAACCTTAATGGATGCAAGAAAGGGGATAAAGTGGAAGGAATTCATCAAATGAAGAAATACCTTCAACATTTTGGGTACTTGAATAATGATGTTCATATTGATTCTGAATCTAGTGATGATGAGTTTGATGAGATCCTGGAGTTCGCTGTCAAAACTTACCAAATCAACTACAATCTTAAGGTCACGGGAACTCTCGACAACTCGACGCTAATGCAAATGTCGAAACCTCGATGTGGGGTTGCGGATATTATCAACGGAAAGACGAGTATGAAATCAGGCAAGAGATTGGTGAACCAACATAGAAAAATAAGTGGTCATTTCCATGAAGTTTCCCACTTTGCTTTCTTTGAAGGAAACCTTAGATGGCCAGATTCCAAATCTCACCTCACTTACGGATTCCTCCCAGGAACTCCGTCAGAGGCGATAAGTCCAGTGAATCGAGCATTTAGGACATGGGCTGCAAACACTCACTTCAGTTTCTCCCAAGAATCAAAGTATGAAAATGCAGACATCAAAATTAGTTTTGAAAAAGGTGACCATGGAGATGGCTTTCCCTTTGACAGTGTTGGAGGGGTGTTAGCCCATGCTTTTGCCCCCACAGATGGGCGATTACATTTCGATGCAGTCGAGCATTGGGCCGATGGTGCTGTTCCTAAGTCTTACGATATGGAGACCGTGGCGCTACATGAGATCGGACACCTTCTCGGCCTTCATCACAGCTCGGTGGAAGGAGCCATTATGTGGCCATCCATCATGGGAGGAGCTACCAAAGGTTTACATGCTGATGATATTGAAGGCATTAAGGTTTTATATACTACTTCTTGA

Protein sequence

MGFQGLSLFFTFTLFLIISLFPLISSSQPKPVDGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVADIINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSEAISPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKGLHADDIEGIKVLYTTS*

Homology

[Close]

BLAST of CSPI05G28560 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.7e-60
Identity = 124/283 (43.82%), Postives = 171/283 (60.42%), Query Frame = 0


Query: 40  NLNGCKKGDKVEGIHQMKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLK 99
           N  GC  G K +G++ +K+Y QHFGY+  + ++    +DD FD+IL+ AV+ YQ N+ L 
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYI-TETNLSGNFTDD-FDDILKNAVEMYQRNFQLN 107

Query: 100 VTGTLDNSTLMQMSKPRCGVADIINGKTSMKSGKRLVNQHRKISG-HFHEVSHFAFFEGN 159
           VTG LD  TL  +  PRCG  D++NG ++M SG++         G  FH V H++FF G 
Sbjct: 108 VTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGE 167

Query: 160 LRWPDSKSHLTYGFLP--GTPSEAISPVNRAFRTWAANTHFSFSQESKYENADIKISFEK 219
            RWP ++  LTY F P      E  S  +RAF  W   T  +F++  ++  +DI I F  
Sbjct: 168 PRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYS 227

Query: 220 GDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHW------ADG--AVPKSYDMETVALH 279
           G+HGDG PFD     LAHAF+P  G  H D  E+W       DG  +V ++ D+E+VA+H
Sbjct: 228 GEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVH 287

Query: 280 EIGHLLGLHHSSVEGAIMWPSIMGGATK-GLHADDIEGIKVLY 311
           EIGHLLGL HSSVEG+IM+P+I  G  K  L  DD+EG++ LY
Sbjct: 288 EIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of CSPI05G28560 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.0e-59
Identity = 135/322 (41.93%), Postives = 182/322 (56.52%), Query Frame = 0


Query: 8   LFFTFTLFLIIS-----LFPLISSSQPKPVDGL-VF---LKNLNGCKKGDKVEGIHQMKK 67
           +F   +LFLI+S      FP  ++  P   +   VF     N  GC  G  V+G++++KK
Sbjct: 5   VFGFLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKK 64

Query: 68  YLQHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCG 127
           Y Q FGY+       S +  D+FD+IL+ AV+ YQ N+NL VTG LD  T+  +  PRCG
Sbjct: 65  YFQRFGYIPETF---SGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCG 124

Query: 128 VADIINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTP- 187
             D++NG + M  G+R   +      H H V  +  F G  RWP ++  LTY F P  P 
Sbjct: 125 NPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPL 184

Query: 188 -SEAISPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAF 247
             E  S  +RAF  W+  T  +F+    +  +DI I F  GDHGDG PFD V G LAHAF
Sbjct: 185 TEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAF 244

Query: 248 APTDGRLHFDAVEHWADG-------AVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPS 307
           +P  G+ H DA E+W          +V  + D+E+VA+HEIGHLLGL HSSVE +IM+P+
Sbjct: 245 SPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPT 304

Query: 308 IMGGATK-GLHADDIEGIKVLY 311
           I  G  K  L  DD+EGI+ LY
Sbjct: 305 ITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of CSPI05G28560 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 220.7 bits (561), Expect = 2.3e-56
Identity = 132/307 (43.00%), Postives = 170/307 (55.37%), Query Frame = 0


Query: 13  TLFLIISLFPLISSSQPKPVDGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYLNNDVHI 72
           TL+ + +  P +S+  P   DG    K       G   +G+  +K Y  H GY+ N  H 
Sbjct: 15  TLYFLATSLPSVSAHGPYAWDGEATYK-FTTYHPGQNYKGLSNVKNYFHHLGYIPNAPHF 74

Query: 73  DSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVADIINGKTSMKSG 132
                DD FD+ L  A+KTYQ NYNL VTG  D +TL Q+  PRCGV DII         
Sbjct: 75  -----DDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVPDII--------- 134

Query: 133 KRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSEAI--SPVNRAFRT 192
              +N ++  S  F  +S + FF+   RW    + LTY F P    +    S + RAF  
Sbjct: 135 ---INTNKTTS--FGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDDTFKSAIARAFSK 194

Query: 193 WAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEH 252
           W    + +F + + YE A+IKI F   +HGD +PFD  GG+L HAFAPTDGR HFDA E+
Sbjct: 195 WTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDADEY 254

Query: 253 W-ADGAVPKS-----YDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATK-GLHADDI 311
           W A G V KS     +D+E+VA+HEIGHLLGL HSS   AIM+PSI     K  L  DDI
Sbjct: 255 WVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDI 301

[Close]

BLAST of CSPI05G28560 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.0e-56
Identity = 136/322 (42.24%), Postives = 179/322 (55.59%), Query Frame = 0


Query: 8   LFFTFTLFLIISLFPLISSSQPKPVDGLVFLKN-------LNGCKKGDKVEGIHQMKKYL 67
           L F FT+  I + F    SS P P+  L   +N       L GC  G+ + G+ ++K+Y 
Sbjct: 9   LIFFFTVNPISAKFYTNVSSIP-PLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYF 68

Query: 68  QHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVA 127
           + FGY+       + +  D+FD++L+ A+ TYQ N+NLKVTG LD+STL Q+ KPRCG  
Sbjct: 69  RRFGYITT-----TGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNP 128

Query: 128 DIINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLP--GTPS 187
           D+I+G + M  GK L                ++FF G  RWP  K  LTY F P      
Sbjct: 129 DLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTD 188

Query: 188 EAISPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAP 247
           E     +RAF  WA  T  +F++      ADI I F  G+HGDG PFD   G LAHA +P
Sbjct: 189 EVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSP 248

Query: 248 TDGRLHFDAVEHW--ADGAVPKS-------YDMETVALHEIGHLLGLHHSSVEGAIMWPS 307
             G LH D  E W  ++G + +         D+E+VA+HEIGHLLGL HSSVE AIM+P+
Sbjct: 249 PTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPA 308

Query: 308 IMGGATK-GLHADDIEGIKVLY 311
           I GG  K  L  DDIEGI+ LY
Sbjct: 309 ISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of CSPI05G28560 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.1e-49
Identity = 117/277 (42.24%), Postives = 150/277 (54.15%), Query Frame = 0


Query: 47  GDKVEGIHQMKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDN 106
           G  V G+ ++K+YL  FGY+N+     SE   D FD  LE A+  YQ N  L +TG LD 
Sbjct: 64  GSHVSGVSELKRYLHRFGYVND----GSEIFSDVFDGPLESAISLYQENLGLPITGRLDT 123

Query: 107 STLMQMSKPRCGVADIINGKTSMKSGKRLVNQHRKISGHF-HEVSHFAFFEGNLRWPDSK 166
           ST+  MS PRCGV+D                 H  I+  F H  +H+ +F G  +W  ++
Sbjct: 124 STVTLMSLPRCGVSD----------------THMTINNDFLHTTAHYTYFNGKPKW--NR 183

Query: 167 SHLTYGF-----LPGTPSEAISPV-NRAFRTWAANTHFSFSQESKYENADIKISFEKGDH 226
             LTY       L    SE +  V  RAF  W++    SF +   +  AD+KI F  GDH
Sbjct: 184 DTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDH 243

Query: 227 GDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHW-----ADGAVPKSYDMETVALHEIGHLL 286
           GDG PFD V G LAHAFAP +GRLH DA E W       G+   + D+E+VA HEIGHLL
Sbjct: 244 GDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLL 303

Query: 287 GLHHSSVEGAIMWPSIMGGATK-GLHADDIEGIKVLY 311
           GL HSS E A+M+PS+     K  L  DD+ G+  LY
Sbjct: 304 GLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of CSPI05G28560 vs. ExPASy TrEMBL
Match: A0A0A0KS47 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.1e-178
Identity = 309/313 (98.72%), Postives = 309/313 (98.72%), Query Frame = 0


Query: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKPVDGLVFLKNLNGCKKGDKVEGIHQMKKYL 60
           MGFQGLSLFFTFTLFLIISLFPLISSSQPKPVDGLVFLKNL GCKKGDKVEGIHQMKKYL
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKPVDGLVFLKNLKGCKKGDKVEGIHQMKKYL 60

Query: 61  QHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVA 120
           QHFGYLNNDVHI SESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVA
Sbjct: 61  QHFGYLNNDVHIRSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVA 120

Query: 121 DIINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSEA 180
           DIINGKTSMKSGKR VNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSEA
Sbjct: 121 DIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSEA 180

Query: 181 ISPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPTD 240
           ISPVNRAF TWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPTD
Sbjct: 181 ISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPTD 240

Query: 241 GRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKGLHA 300
           GRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKGLHA
Sbjct: 241 GRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKGLHA 300

Query: 301 DDIEGIKVLYTTS 314
           DDIEGIKVLYTTS
Sbjct: 301 DDIEGIKVLYTTS 313

[Close]

BLAST of CSPI05G28560 vs. ExPASy TrEMBL
Match: A0A1S3C925 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498039 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.9e-155
Identity = 262/282 (92.91%), Postives = 273/282 (96.81%), Query Frame = 0


Query: 32  VDGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKT 91
           +DGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGY+NNDVHI S+SSDDEFDEILEFAVKT
Sbjct: 1   MDGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYMNNDVHIHSKSSDDEFDEILEFAVKT 60

Query: 92  YQINYNLKVTGTLDNSTLMQMSKPRCGVADIINGKTSMKSGKRLVNQHRKISGHFHEVSH 151
           YQINYNLK TGTLDNST+ QMSKPRCGVADIING+T MKSGKRLVN+HRKI GHFHEVSH
Sbjct: 61  YQINYNLKATGTLDNSTISQMSKPRCGVADIINGRTGMKSGKRLVNRHRKIIGHFHEVSH 120

Query: 152 FAFFEGNLRWPDSKSHLTYGFLPGTPSEAISPVNRAFRTWAANTHFSFSQESKYENADIK 211
           FAFFEGNL+WP+SKSHLTYGFLPGTPSEAISPVNRAF TWAANTHFSFSQES YENADIK
Sbjct: 121 FAFFEGNLKWPNSKSHLTYGFLPGTPSEAISPVNRAFTTWAANTHFSFSQESNYENADIK 180

Query: 212 ISFEKGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIG 271
           ++FE+GDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVP SYDMETVALHEIG
Sbjct: 181 VTFERGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPNSYDMETVALHEIG 240

Query: 272 HLLGLHHSSVEGAIMWPSIMGGATKGLHADDIEGIKVLYTTS 314
           HLLGLHHSSVEGAIMWPSIMGG TKGLHADDIEGIKVLYTTS
Sbjct: 241 HLLGLHHSSVEGAIMWPSIMGGTTKGLHADDIEGIKVLYTTS 282

[Close]

BLAST of CSPI05G28560 vs. ExPASy TrEMBL
Match: A0A5A7T7T1 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00140 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.1e-154
Identity = 262/282 (92.91%), Postives = 272/282 (96.45%), Query Frame = 0


Query: 32  VDGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKT 91
           +DGLVFLKNLNGCKKGDKVEGIH MKKYLQHFGY+NNDVHI S+SSDDEFDEILEFAVKT
Sbjct: 1   MDGLVFLKNLNGCKKGDKVEGIHLMKKYLQHFGYMNNDVHIHSKSSDDEFDEILEFAVKT 60

Query: 92  YQINYNLKVTGTLDNSTLMQMSKPRCGVADIINGKTSMKSGKRLVNQHRKISGHFHEVSH 151
           YQINYNLK TGTLDNST+ QMSKPRCGVADIING+T MKSGKRLVN+HRKI GHFHEVSH
Sbjct: 61  YQINYNLKATGTLDNSTISQMSKPRCGVADIINGRTGMKSGKRLVNRHRKIIGHFHEVSH 120

Query: 152 FAFFEGNLRWPDSKSHLTYGFLPGTPSEAISPVNRAFRTWAANTHFSFSQESKYENADIK 211
           FAFFEGNL+WP+SKSHLTYGFLPGTPSEAISPVNRAF TWAANTHFSFSQES YENADIK
Sbjct: 121 FAFFEGNLKWPNSKSHLTYGFLPGTPSEAISPVNRAFTTWAANTHFSFSQESNYENADIK 180

Query: 212 ISFEKGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIG 271
           +SFE+GDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVP SYDMETVALHEIG
Sbjct: 181 VSFERGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPNSYDMETVALHEIG 240

Query: 272 HLLGLHHSSVEGAIMWPSIMGGATKGLHADDIEGIKVLYTTS 314
           HLLGLHHSSVEGAIMWPSIMGG TKGLHADDIEGIKVLYTTS
Sbjct: 241 HLLGLHHSSVEGAIMWPSIMGGTTKGLHADDIEGIKVLYTTS 282

[Close]

BLAST of CSPI05G28560 vs. ExPASy TrEMBL
Match: A0A6J1FDT9 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 PE=3 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 9.6e-130
Identity = 231/312 (74.04%), Postives = 261/312 (83.65%), Query Frame = 0


Query: 4   QGLSLFFTFTLFLIISLFPLISSSQPKPVD--GLVFLKNLNGCKKGDKVEGIHQMKKYLQ 63
           Q LS F   +LFL+I+LFPLISSS   P    GL+FL NLNGCKKGD VEG+HQMK+YL+
Sbjct: 5   QALS-FLASSLFLLIALFPLISSSHQYPAQSHGLLFLNNLNGCKKGDTVEGLHQMKRYLR 64

Query: 64  HFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVAD 123
            FGYLN  V   S++ DDEFDE+LE A++TYQ+NYNL+ TGTLD STL  MSKPRCGVAD
Sbjct: 65  RFGYLNR-VQNHSKTYDDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVAD 124

Query: 124 IINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSEAI 183
           + +GKT M SGK++VN+HRKISG FH VSHFAFF+GN +WP SKSHLTYGFLPGTP+EAI
Sbjct: 125 VFHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTEAI 184

Query: 184 SPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPTDG 243
           +PV RAF TWAANTHF+FS  S YE ADIKISFE GDHGDG  FD VGGV+AHAF+PTDG
Sbjct: 185 APVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDG 244

Query: 244 RLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKGLHAD 303
           RLHFDAVE WA GA+P S+DMETVALHEIGHLLGL HSSVEGAIMWP+IM GATKGLHAD
Sbjct: 245 RLHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLHAD 304

Query: 304 DIEGIKVLYTTS 314
           DI GI  LYTT+
Sbjct: 305 DIAGINALYTTT 314

[Close]

BLAST of CSPI05G28560 vs. ExPASy TrEMBL
Match: A0A6J1JTF5 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.1e-129
Identity = 231/312 (74.04%), Postives = 261/312 (83.65%), Query Frame = 0


Query: 4   QGLSLFFTFTLFLIISLFPLISSSQPKPV--DGLVFLKNLNGCKKGDKVEGIHQMKKYLQ 63
           Q LS FF  +LFL+I+LFPLISSS   P    GL+FL NLNGCKKGD VEG+HQMK+YL 
Sbjct: 5   QALS-FFASSLFLLITLFPLISSSPQYPAHSHGLLFLNNLNGCKKGDTVEGLHQMKRYLC 64

Query: 64  HFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVAD 123
            FGYL++ V   S++ DDEFDE+LE A++TYQ+NYNL+ TGTLD STL  MSKPRCGVAD
Sbjct: 65  RFGYLSH-VQNHSKTYDDEFDELLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVAD 124

Query: 124 IINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSEAI 183
           + +GKT M SGK++VN+HRKIS HFH VSHFAFF+GN +WP SKSHL YGFLPGTP EAI
Sbjct: 125 VFHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYGFLPGTPIEAI 184

Query: 184 SPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPTDG 243
           +PV RAF TWAANTHF+FSQ S YE ADIKISFE GDHGDG  FD VGGV+AHAF+PTDG
Sbjct: 185 APVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDG 244

Query: 244 RLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKGLHAD 303
           RLHFDA+E WA GAVP S+DMETVALHEIGHLLGLHHSSVEGAIMWP+IM GATKGLHAD
Sbjct: 245 RLHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIMEGATKGLHAD 304

Query: 304 DIEGIKVLYTTS 314
           DI GI  LYT +
Sbjct: 305 DIAGINALYTAT 314

[Close]

BLAST of CSPI05G28560 vs. NCBI nr
Match: XP_004142457.2 (metalloendoproteinase 3-MMP [Cucumis sativus])

HSP 1 Score: 635.6 bits (1638), Expect = 2.3e-178
Identity = 309/313 (98.72%), Postives = 309/313 (98.72%), Query Frame = 0


Query: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKPVDGLVFLKNLNGCKKGDKVEGIHQMKKYL 60
           MGFQGLSLFFTFTLFLIISLFPLISSSQPKPVDGLVFLKNL GCKKGDKVEGIHQMKKYL
Sbjct: 4   MGFQGLSLFFTFTLFLIISLFPLISSSQPKPVDGLVFLKNLKGCKKGDKVEGIHQMKKYL 63

Query: 61  QHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVA 120
           QHFGYLNNDVHI SESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVA
Sbjct: 64  QHFGYLNNDVHIRSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVA 123

Query: 121 DIINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSEA 180
           DIINGKTSMKSGKR VNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSEA
Sbjct: 124 DIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTPSEA 183

Query: 181 ISPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPTD 240
           ISPVNRAF TWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPTD
Sbjct: 184 ISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAPTD 243

Query: 241 GRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKGLHA 300
           GRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKGLHA
Sbjct: 244 GRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKGLHA 303

Query: 301 DDIEGIKVLYTTS 314
           DDIEGIKVLYTTS
Sbjct: 304 DDIEGIKVLYTTS 316

[Close]

BLAST of CSPI05G28560 vs. NCBI nr
Match: XP_008458713.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo])

HSP 1 Score: 557.4 bits (1435), Expect = 8.0e-155
Identity = 262/282 (92.91%), Postives = 273/282 (96.81%), Query Frame = 0


Query: 32  VDGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKT 91
           +DGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGY+NNDVHI S+SSDDEFDEILEFAVKT
Sbjct: 1   MDGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYMNNDVHIHSKSSDDEFDEILEFAVKT 60

Query: 92  YQINYNLKVTGTLDNSTLMQMSKPRCGVADIINGKTSMKSGKRLVNQHRKISGHFHEVSH 151
           YQINYNLK TGTLDNST+ QMSKPRCGVADIING+T MKSGKRLVN+HRKI GHFHEVSH
Sbjct: 61  YQINYNLKATGTLDNSTISQMSKPRCGVADIINGRTGMKSGKRLVNRHRKIIGHFHEVSH 120

Query: 152 FAFFEGNLRWPDSKSHLTYGFLPGTPSEAISPVNRAFRTWAANTHFSFSQESKYENADIK 211
           FAFFEGNL+WP+SKSHLTYGFLPGTPSEAISPVNRAF TWAANTHFSFSQES YENADIK
Sbjct: 121 FAFFEGNLKWPNSKSHLTYGFLPGTPSEAISPVNRAFTTWAANTHFSFSQESNYENADIK 180

Query: 212 ISFEKGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIG 271
           ++FE+GDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVP SYDMETVALHEIG
Sbjct: 181 VTFERGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPNSYDMETVALHEIG 240

Query: 272 HLLGLHHSSVEGAIMWPSIMGGATKGLHADDIEGIKVLYTTS 314
           HLLGLHHSSVEGAIMWPSIMGG TKGLHADDIEGIKVLYTTS
Sbjct: 241 HLLGLHHSSVEGAIMWPSIMGGTTKGLHADDIEGIKVLYTTS 282

[Close]

BLAST of CSPI05G28560 vs. NCBI nr
Match: KAA0037429.1 (metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK00222.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 555.8 bits (1431), Expect = 2.3e-154
Identity = 262/282 (92.91%), Postives = 272/282 (96.45%), Query Frame = 0


Query: 32  VDGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKT 91
           +DGLVFLKNLNGCKKGDKVEGIH MKKYLQHFGY+NNDVHI S+SSDDEFDEILEFAVKT
Sbjct: 1   MDGLVFLKNLNGCKKGDKVEGIHLMKKYLQHFGYMNNDVHIHSKSSDDEFDEILEFAVKT 60

Query: 92  YQINYNLKVTGTLDNSTLMQMSKPRCGVADIINGKTSMKSGKRLVNQHRKISGHFHEVSH 151
           YQINYNLK TGTLDNST+ QMSKPRCGVADIING+T MKSGKRLVN+HRKI GHFHEVSH
Sbjct: 61  YQINYNLKATGTLDNSTISQMSKPRCGVADIINGRTGMKSGKRLVNRHRKIIGHFHEVSH 120

Query: 152 FAFFEGNLRWPDSKSHLTYGFLPGTPSEAISPVNRAFRTWAANTHFSFSQESKYENADIK 211
           FAFFEGNL+WP+SKSHLTYGFLPGTPSEAISPVNRAF TWAANTHFSFSQES YENADIK
Sbjct: 121 FAFFEGNLKWPNSKSHLTYGFLPGTPSEAISPVNRAFTTWAANTHFSFSQESNYENADIK 180

Query: 212 ISFEKGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIG 271
           +SFE+GDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVP SYDMETVALHEIG
Sbjct: 181 VSFERGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPNSYDMETVALHEIG 240

Query: 272 HLLGLHHSSVEGAIMWPSIMGGATKGLHADDIEGIKVLYTTS 314
           HLLGLHHSSVEGAIMWPSIMGG TKGLHADDIEGIKVLYTTS
Sbjct: 241 HLLGLHHSSVEGAIMWPSIMGGTTKGLHADDIEGIKVLYTTS 282

[Close]

BLAST of CSPI05G28560 vs. NCBI nr
Match: XP_038891009.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 547.4 bits (1409), Expect = 8.3e-152
Identity = 269/316 (85.13%), Postives = 291/316 (92.09%), Query Frame = 0


Query: 1   MGFQGLSL-FFTFTLFLIISLFPLISSSQPKPVD--GLVFLKNLNGCKKGDKVEGIHQMK 60
           MGFQ +SL FFTFTLFL+I+LFP ISSSQPKPVD  GLVFLKNL GCKKGDKVEGIHQMK
Sbjct: 4   MGFQEISLSFFTFTLFLLITLFPSISSSQPKPVDSHGLVFLKNLIGCKKGDKVEGIHQMK 63

Query: 61  KYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRC 120
           KYL HFGYL NDVHI S+S++DEFDEILE A+KTYQINYNLK TGTLDN+TL Q+ K RC
Sbjct: 64  KYLHHFGYL-NDVHIHSKSNEDEFDEILESAIKTYQINYNLKATGTLDNTTLAQILKSRC 123

Query: 121 GVADIINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTP 180
           GVADIINGKT MKSG ++VNQHRKISG FHEVSHFAFFEG+L+WPDSKSHLTYGFLP TP
Sbjct: 124 GVADIINGKTRMKSGTKVVNQHRKISGPFHEVSHFAFFEGSLKWPDSKSHLTYGFLPKTP 183

Query: 181 SEAISPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFA 240
           S+A+ PV+RAFRTWAANTHFSFSQES YE+ADIKISFE+GDHGDG PFDSVGGVLAHAFA
Sbjct: 184 SKAVDPVSRAFRTWAANTHFSFSQESDYESADIKISFERGDHGDGSPFDSVGGVLAHAFA 243

Query: 241 PTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKG 300
           PTDGRLHFDAVEHWA GAVP SYD+ETVALHEIGHLLGL+HSSVEGAIMWPSIMGG TKG
Sbjct: 244 PTDGRLHFDAVEHWAIGAVPNSYDVETVALHEIGHLLGLYHSSVEGAIMWPSIMGGTTKG 303

Query: 301 LHADDIEGIKVLYTTS 314
           LHADDIEGIK LYT++
Sbjct: 304 LHADDIEGIKALYTST 318

[Close]

BLAST of CSPI05G28560 vs. NCBI nr
Match: KAE8648906.1 (hypothetical protein Csa_009291 [Cucumis sativus])

HSP 1 Score: 531.2 bits (1367), Expect = 6.1e-147
Identity = 255/258 (98.84%), Postives = 255/258 (98.84%), Query Frame = 0


Query: 56  MKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKP 115
           MKKYLQHFGYLNNDVHI SESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKP
Sbjct: 1   MKKYLQHFGYLNNDVHIRSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKP 60

Query: 116 RCGVADIINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPG 175
           RCGVADIINGKTSMKSGKR VNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPG
Sbjct: 61  RCGVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPG 120

Query: 176 TPSEAISPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHA 235
           TPSEAISPVNRAF TWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHA
Sbjct: 121 TPSEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHA 180

Query: 236 FAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGAT 295
           FAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGAT
Sbjct: 181 FAPTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGAT 240

Query: 296 KGLHADDIEGIKVLYTTS 314
           KGLHADDIEGIKVLYTTS
Sbjct: 241 KGLHADDIEGIKVLYTTS 258

[Close]

BLAST of CSPI05G28560 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 233.8 bits (595), Expect = 1.9e-61
Identity = 124/283 (43.82%), Postives = 171/283 (60.42%), Query Frame = 0


Query: 40  NLNGCKKGDKVEGIHQMKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLK 99
           N  GC  G K +G++ +K+Y QHFGY+  + ++    +DD FD+IL+ AV+ YQ N+ L 
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYI-TETNLSGNFTDD-FDDILKNAVEMYQRNFQLN 107

Query: 100 VTGTLDNSTLMQMSKPRCGVADIINGKTSMKSGKRLVNQHRKISG-HFHEVSHFAFFEGN 159
           VTG LD  TL  +  PRCG  D++NG ++M SG++         G  FH V H++FF G 
Sbjct: 108 VTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGE 167

Query: 160 LRWPDSKSHLTYGFLP--GTPSEAISPVNRAFRTWAANTHFSFSQESKYENADIKISFEK 219
            RWP ++  LTY F P      E  S  +RAF  W   T  +F++  ++  +DI I F  
Sbjct: 168 PRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYS 227

Query: 220 GDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHW------ADG--AVPKSYDMETVALH 279
           G+HGDG PFD     LAHAF+P  G  H D  E+W       DG  +V ++ D+E+VA+H
Sbjct: 228 GEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVH 287

Query: 280 EIGHLLGLHHSSVEGAIMWPSIMGGATK-GLHADDIEGIKVLY 311
           EIGHLLGL HSSVEG+IM+P+I  G  K  L  DD+EG++ LY
Sbjct: 288 EIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of CSPI05G28560 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 231.9 bits (590), Expect = 7.2e-61
Identity = 135/322 (41.93%), Postives = 182/322 (56.52%), Query Frame = 0


Query: 8   LFFTFTLFLIIS-----LFPLISSSQPKPVDGL-VF---LKNLNGCKKGDKVEGIHQMKK 67
           +F   +LFLI+S      FP  ++  P   +   VF     N  GC  G  V+G++++KK
Sbjct: 5   VFGFLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKK 64

Query: 68  YLQHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCG 127
           Y Q FGY+       S +  D+FD+IL+ AV+ YQ N+NL VTG LD  T+  +  PRCG
Sbjct: 65  YFQRFGYIPETF---SGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCG 124

Query: 128 VADIINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTP- 187
             D++NG + M  G+R   +      H H V  +  F G  RWP ++  LTY F P  P 
Sbjct: 125 NPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPL 184

Query: 188 -SEAISPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAF 247
             E  S  +RAF  W+  T  +F+    +  +DI I F  GDHGDG PFD V G LAHAF
Sbjct: 185 TEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAF 244

Query: 248 APTDGRLHFDAVEHWADG-------AVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPS 307
           +P  G+ H DA E+W          +V  + D+E+VA+HEIGHLLGL HSSVE +IM+P+
Sbjct: 245 SPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPT 304

Query: 308 IMGGATK-GLHADDIEGIKVLY 311
           I  G  K  L  DD+EGI+ LY
Sbjct: 305 ITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of CSPI05G28560 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 220.3 bits (560), Expect = 2.2e-57
Identity = 136/322 (42.24%), Postives = 179/322 (55.59%), Query Frame = 0


Query: 8   LFFTFTLFLIISLFPLISSSQPKPVDGLVFLKN-------LNGCKKGDKVEGIHQMKKYL 67
           L F FT+  I + F    SS P P+  L   +N       L GC  G+ + G+ ++K+Y 
Sbjct: 9   LIFFFTVNPISAKFYTNVSSIP-PLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYF 68

Query: 68  QHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRCGVA 127
           + FGY+       + +  D+FD++L+ A+ TYQ N+NLKVTG LD+STL Q+ KPRCG  
Sbjct: 69  RRFGYITT-----TGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNP 128

Query: 128 DIINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLP--GTPS 187
           D+I+G + M  GK L                ++FF G  RWP  K  LTY F P      
Sbjct: 129 DLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTD 188

Query: 188 EAISPVNRAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFAP 247
           E     +RAF  WA  T  +F++      ADI I F  G+HGDG PFD   G LAHA +P
Sbjct: 189 EVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSP 248

Query: 248 TDGRLHFDAVEHW--ADGAVPKS-------YDMETVALHEIGHLLGLHHSSVEGAIMWPS 307
             G LH D  E W  ++G + +         D+E+VA+HEIGHLLGL HSSVE AIM+P+
Sbjct: 249 PTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPA 308

Query: 308 IMGGATK-GLHADDIEGIKVLY 311
           I GG  K  L  DDIEGI+ LY
Sbjct: 309 ISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of CSPI05G28560 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 196.1 bits (497), Expect = 4.4e-50
Identity = 117/277 (42.24%), Postives = 150/277 (54.15%), Query Frame = 0


Query: 47  GDKVEGIHQMKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDN 106
           G  V G+ ++K+YL  FGY+N+     SE   D FD  LE A+  YQ N  L +TG LD 
Sbjct: 64  GSHVSGVSELKRYLHRFGYVND----GSEIFSDVFDGPLESAISLYQENLGLPITGRLDT 123

Query: 107 STLMQMSKPRCGVADIINGKTSMKSGKRLVNQHRKISGHF-HEVSHFAFFEGNLRWPDSK 166
           ST+  MS PRCGV+D                 H  I+  F H  +H+ +F G  +W  ++
Sbjct: 124 STVTLMSLPRCGVSD----------------THMTINNDFLHTTAHYTYFNGKPKW--NR 183

Query: 167 SHLTYGF-----LPGTPSEAISPV-NRAFRTWAANTHFSFSQESKYENADIKISFEKGDH 226
             LTY       L    SE +  V  RAF  W++    SF +   +  AD+KI F  GDH
Sbjct: 184 DTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDH 243

Query: 227 GDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHW-----ADGAVPKSYDMETVALHEIGHLL 286
           GDG PFD V G LAHAFAP +GRLH DA E W       G+   + D+E+VA HEIGHLL
Sbjct: 244 GDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLL 303

Query: 287 GLHHSSVEGAIMWPSIMGGATK-GLHADDIEGIKVLY 311
           GL HSS E A+M+PS+     K  L  DD+ G+  LY
Sbjct: 304 GLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of CSPI05G28560 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 172.9 bits (437), Expect = 4.0e-43
Identity = 110/273 (40.29%), Postives = 148/273 (54.21%), Query Frame = 0


Query: 53  IHQMKKYLQHFGYLNNDVHIDSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQM 112
           I ++K++LQ +GYL      + ES D  F++    A+  YQ N  L +TG  D+ TL Q+
Sbjct: 50  IPEIKRHLQQYGYLPQ----NKESDDVSFEQ----ALVRYQKNLGLPITGKPDSDTLSQI 109

Query: 113 SKPRCGVADIINGKTSMKSGKRLVNQHRKISGHFHEVSHFAFFEGNLRWP-DSKSHLTYG 172
             PRCG  D +  KT+                 FH    + +F G  RW  D    LTY 
Sbjct: 110 LLPRCGFPDDVEPKTA----------------PFHTGKKYVYFPGRPRWTRDVPLKLTYA 169

Query: 173 FLPGTPSEAISPVN------RAFRTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPF 232
           F     +  ++P +      RAF  WA+    SF +   Y  ADIKI F  GDHGDG PF
Sbjct: 170 FSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPF 229

Query: 233 DSVGGVLAHAFAPTDGRLHFDAVEHWA-DGAVPKS---YDMETVALHEIGHLLGLHHSSV 292
           D V GVLAH F+P +GRLH D  E WA D    KS    D+E+VA+HEIGH+LGL HSSV
Sbjct: 230 DGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSV 289

Query: 293 EGAIMWPSIMGGATK-GLHADDIEGIKVLYTTS 314
           + A M+P++   + K  L+ DD+ G++ LY T+
Sbjct: 290 KDAAMYPTLKPRSKKVNLNMDDVVGVQSLYGTN 298

The following BLAST results are available for this feature:

BLAST of CSPI05G28560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XF51	2.7e-60	43.82	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
O04529	1.0e-59	41.93	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
P29136	2.3e-56	43.00	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q9ZUJ5	3.0e-56	42.24	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O23507	6.1e-49	42.24	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of CSPI05G28560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KS47	1.1e-178	98.72	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 S...	[more]
A0A1S3C925	3.9e-155	92.91	metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498039 PE=3 SV...	[more]
A0A5A7T7T1	1.1e-154	92.91	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1FDT9	9.6e-130	74.04	metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 P...	[more]
A0A6J1JTF5	2.1e-129	74.04	metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=...	[more]

BLAST of CSPI05G28560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142457.2	2.3e-178	98.72	metalloendoproteinase 3-MMP [Cucumis sativus]	[more]
XP_008458713.1	8.0e-155	92.91	PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo]	[more]
KAA0037429.1	2.3e-154	92.91	metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK00222.1 metalloe...	[more]
XP_038891009.1	8.3e-152	85.13	metalloendoproteinase 2-MMP-like [Benincasa hispida]	[more]
KAE8648906.1	6.1e-147	98.84	hypothetical protein Csa_009291 [Cucumis sativus]	[more]

BLAST of CSPI05G28560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24140.1	1.9e-61	43.82	Matrixin family protein	[more]
AT1G70170.1	7.2e-61	41.93	matrix metalloproteinase	[more]
AT1G59970.1	2.2e-57	42.24	Matrixin family protein	[more]
AT4G16640.1	4.4e-50	42.24	Matrixin family protein	[more]
AT2G45040.1	4.0e-43	40.29	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 298..311 score: 51.66 coord: 265..290 score: 57.13 coord: 112..125 score: 52.09 coord: 184..199 score: 33.08 coord: 208..236 score: 60.72
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 157..312 e-value: 2.8E-30 score: 116.6
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 40..313 e-value: 5.8E-75 score: 253.9
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 51..112 e-value: 1.0E-11 score: 44.9
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 164..310 e-value: 5.7E-43 score: 146.5
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 9..311
None	No IPR available	PANTHER	PTHR10201:SF269	METALLOENDOPROTEINASE 3-MMP-LIKE	coord: 9..311
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 154..311
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 160..310 e-value: 2.26988E-55 score: 175.085
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 44..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G28560.1	CSPI05G28560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity