CSPI05G28140 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G28140
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRNA exonuclease 4
LocationChr5: 26670092 .. 26672586 (+)
RNA-Seq ExpressionCSPI05G28140
SyntenyCSPI05G28140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTCAGGGCCATTGCCAAAGCAAGAATGCAAAAACATATTCAATGCAAGAGGATGCAAAATCTGCTTAGCCATCTTTGAGAGTCCACTCCAGCTCAACCTTCACCAAGAAAGTTGCCAACTTAGGCCTATAAATTTTGTAAGTGTTCGTCCTGTTTTACGCATTTTTTATATCTCCATGCCTTTTACTCGATTTATGCATTTGAAAGTATATGAACTAAATGTGTTTCCAATTAAAGTTTAATGATCAAATTATAACTTTTATGAAAGTTTAAGAATTGTGTAGGTATTTTTTTAACACGAAAAACTTTAACTTTTTAGAAAAATCTAAAAATAGGTTGCAACAAGTAACAAATTATTATCAAATAATTGATTGAGATGCATGAAAAATTTATATTAATCAAAGTAAGCATTATATCTGATACTTGTTATATCAATTTTAAATTTAGACGTTCACAATTTTCTAAACTATATGTATGAAAAGGAGAAATAAAACTTCACATTAATGTTAAAAGAATTTTACCCCTATTAAAAGAAAAAGAGTTTATTATACTCTCAAGGTGCTAGAAATCTTATTTCGACTGATTGCAGAGTCATGAAAATAGGGCACGTTTGGGGAAGACAGAAAGTGGTGGAGCAAAAGGCAATAACGAAGCAGTGGCATTGAGTTGCACCATGGTGGGCGCTGGCATCGACGGCTCCCTAAATATTTGCGTTAGGGTTTGTGTTGTTGACCAGAACGAGTCCCTCATCTTTTCTACTTATGTCAACCCAACCCTTCCCATCACAAACTATAGGTATATATCCAATTTCTCCTTCTTCTTTTTTACAGTAATAACTATTGTTGTAATTTCTTCTGCTTTTTTGTTTTTTTTTTTCCATTTTAATAAAGAAACACAGTAATAAGATTATATTTAGACCCCGTCTGATAAATTTTTAGTTTTTGAAAATCCAAAGTCCTTCGAGAATTGTTTTTGCATCTTGAAGACTACTATCTATTTATCACGAATAGTTAGAGAGTGTGATATTTTATTATATTTGTAAATAATTTGAAAAGATTCCTGATTTAAAACAAAATTAGGTTTTGATATTAATTAGATTGTGTGGGTGTAGGTATGAATTCACAGGTGTTCGTCCAGAACACTTGAGAGATGCAATGCCATTGAAGCAAGTCCAGAAGAAAATTCAAGAGTTTCTATGCAATGGAGAACAAATGTGGAAGATTCGTCCTGGATCTACTGGAAGAGCAAGAATTCTTGTGGGTCATGGCCTTCAAGAATACCTTACATCTTTGCAAATTGACTACCCTCCCATTATGATCAGGTAAAATGTTTATATCACTGCATCTCGAGCTACATGCATATCCATTTTTAGGCCGTTTCTTAACAAGGAAAATATTTTTTAACATATTTTCTCTACATTCTTAACAGGGAAAATAAAAGTCGAATTTCTAATGAATAATAATTTATTTAAACTGCAGGGATACTGCTAAATATCCACCATTGATGAAGACAAGCAAGCTAAGCAATTCTCTCCACTGTTTGACACAAACATATCTTGGGTAAAGTTTTCCACATGCATAACCCCTGCCTCCCCCAATAATATTGTTATTTTTTTCTTCCTTCACTAGCTAGATACCTCAAAAGTAGTAGTAAAAATATATATATTTGGTAACAATTTTGTTTTAGATATGAAAAATAGATATCAATCCATAAAAGAAATATATATTATAGATGAAAGTAGGGTTTACAAGAGTAAGTTTTTAAAATGAAAAAATAAATAGGACAGCTTAGATGATTTATTGTTAAATCAATTTAATTAAAGCTTTAAGCTGGTGGTCAACTGGGATAAGCACTTTAACGGTCCTCTCACTTTTTTTTTTTTTTTTTTTTATTTCATCGTCATGATACAGAGCAGAAAATCAAATTGCTAATCTTCCACTATGGTAGATTTAGATAGAAAATTATATTAAATAAAAATTTAATTTTTAATATATCATGTCAAATCATCAATCAACTCGAATAATTAAATTAGTTAATGGATTTTGAGTAATTTAATTATATATACAATACTCTAACATTTATAAACAAATTAAAGGTAACTAAACAAAATATTTTAGAATGTTAATCAATAAATGTATTAGAATAGATTTGTTGGAATGAATTAAAAAATTTAATAAGTTTATAAACTTTAAACATTTTACGTCTAATTATATGGTATTGTATATTTTTTGGAATTTTGTTATTCAGATACAGTATGGAAAATGGGAAGAGATTAGAAGGTTATGAGGAGTGTGTTGCAACAATGAGATTGTACATGAGAATGAAATCTCAAGTTCACAAAAGAGAGGAATATCCATTGGCTTCTGATCCACAAAATAAGTTTAATTTTGCAAATTGGAAGCAACTTGAGCTTGAGAAGATGACACCCCAACAGTTGTTGCAAATTTCAAGATCTGATTACTATTGTTGGTGTTTGGATTCCACCCTTTAA

mRNA sequence

ATGTTTTCAGGGCCATTGCCAAAGCAAGAATGCAAAAACATATTCAATGCAAGAGGATGCAAAATCTGCTTAGCCATCTTTGAGAGTCCACTCCAGCTCAACCTTCACCAAGAAAGTTGCCAACTTAGGCCTATAAATTTTAGTCATGAAAATAGGGCACGTTTGGGGAAGACAGAAAGTGGTGGAGCAAAAGGCAATAACGAAGCAGTGGCATTGAGTTGCACCATGGTGGGCGCTGGCATCGACGGCTCCCTAAATATTTGCGTTAGGGTTTGTGTTGTTGACCAGAACGAGTCCCTCATCTTTTCTACTTATGTCAACCCAACCCTTCCCATCACAAACTATAGGTATGAATTCACAGGTGTTCGTCCAGAACACTTGAGAGATGCAATGCCATTGAAGCAAGTCCAGAAGAAAATTCAAGAGTTTCTATGCAATGGAGAACAAATGTGGAAGATTCGTCCTGGATCTACTGGAAGAGCAAGAATTCTTGTGGGTCATGGCCTTCAAGAATACCTTACATCTTTGCAAATTGACTACCCTCCCATTATGATCAGGGATACTGCTAAATATCCACCATTGATGAAGACAAGCAAGCTAAGCAATTCTCTCCACTGTTTGACACAAACATATCTTGGATACAGTATGGAAAATGGGAAGAGATTAGAAGGTTATGAGGAGTGTGTTGCAACAATGAGATTGTACATGAGAATGAAATCTCAAGTTCACAAAAGAGAGGAATATCCATTGGCTTCTGATCCACAAAATAAGTTTAATTTTGCAAATTGGAAGCAACTTGAGCTTGAGAAGATGACACCCCAACAGTTGTTGCAAATTTCAAGATCTGATTACTATTGTTGGTGTTTGGATTCCACCCTTTAA

Coding sequence (CDS)

ATGTTTTCAGGGCCATTGCCAAAGCAAGAATGCAAAAACATATTCAATGCAAGAGGATGCAAAATCTGCTTAGCCATCTTTGAGAGTCCACTCCAGCTCAACCTTCACCAAGAAAGTTGCCAACTTAGGCCTATAAATTTTAGTCATGAAAATAGGGCACGTTTGGGGAAGACAGAAAGTGGTGGAGCAAAAGGCAATAACGAAGCAGTGGCATTGAGTTGCACCATGGTGGGCGCTGGCATCGACGGCTCCCTAAATATTTGCGTTAGGGTTTGTGTTGTTGACCAGAACGAGTCCCTCATCTTTTCTACTTATGTCAACCCAACCCTTCCCATCACAAACTATAGGTATGAATTCACAGGTGTTCGTCCAGAACACTTGAGAGATGCAATGCCATTGAAGCAAGTCCAGAAGAAAATTCAAGAGTTTCTATGCAATGGAGAACAAATGTGGAAGATTCGTCCTGGATCTACTGGAAGAGCAAGAATTCTTGTGGGTCATGGCCTTCAAGAATACCTTACATCTTTGCAAATTGACTACCCTCCCATTATGATCAGGGATACTGCTAAATATCCACCATTGATGAAGACAAGCAAGCTAAGCAATTCTCTCCACTGTTTGACACAAACATATCTTGGATACAGTATGGAAAATGGGAAGAGATTAGAAGGTTATGAGGAGTGTGTTGCAACAATGAGATTGTACATGAGAATGAAATCTCAAGTTCACAAAAGAGAGGAATATCCATTGGCTTCTGATCCACAAAATAAGTTTAATTTTGCAAATTGGAAGCAACTTGAGCTTGAGAAGATGACACCCCAACAGTTGTTGCAAATTTCAAGATCTGATTACTATTGTTGGTGTTTGGATTCCACCCTTTAA

Protein sequence

MFSGPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSHENRARLGKTESGGAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDSTL*
Homology
BLAST of CSPI05G28140 vs. ExPASy Swiss-Prot
Match: Q9GZR2 (RNA exonuclease 4 OS=Homo sapiens OX=9606 GN=REXO4 PE=1 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 2.7e-22
Identity = 67/195 (34.36%), Postives = 99/195 (50.77%), Query Frame = 0

Query: 65  GNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVRP 124
           G   A+AL C MVG G  G  ++  RV +V+Q    ++  YV PT P+T+YR   +G+RP
Sbjct: 239 GLTRALALDCEMVGVGPKGEESMAARVSIVNQYGKCVYDKYVKPTEPVTDYRTAVSGIRP 298

Query: 125 EHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPIM 184
           E+L+    L+ VQK++ E L               + RILVGH L   L  L +D+P   
Sbjct: 299 ENLKQGEELEVVQKEVAEML---------------KGRILVGHALHNDLKVLFLDHPKKK 358

Query: 185 IRDTAKYPPLMKTSKLSN-SLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVH 244
           IRDT KY P     K    SL  L++  LG  ++  +     ++  A MRLY+ +K +  
Sbjct: 359 IRDTQKYKPFKSQVKSGRPSLRLLSEKILGLQVQQAEHC-SIQDAQAAMRLYVMVKKEWE 417

Query: 245 K--REEYPLASDPQN 257
              R+  PL + P +
Sbjct: 419 SMARDRRPLLTAPDH 417

BLAST of CSPI05G28140 vs. ExPASy Swiss-Prot
Match: Q6PAQ4 (RNA exonuclease 4 OS=Mus musculus OX=10090 GN=Rexo4 PE=2 SV=2)

HSP 1 Score: 100.9 bits (250), Expect = 2.5e-20
Identity = 61/178 (34.27%), Postives = 92/178 (51.69%), Query Frame = 0

Query: 65  GNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVRP 124
           G  +A+AL C MVG G  G  +I  RV +V+Q    ++  YV PT P+T+YR   +G+RP
Sbjct: 249 GLTKALALDCEMVGVGPKGEESIAARVSIVNQYGKCVYDKYVKPTEPVTDYRTAVSGIRP 308

Query: 125 EHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPIM 184
           E+L+     + V+K++ E L               + RILVGH L   L  L +D+P   
Sbjct: 309 ENLKQGEEFEVVKKEVAEML---------------KGRILVGHALHNDLKVLFLDHPKKK 368

Query: 185 IRDTAKYPPLMKTSKLSN-SLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMKSQ 242
           IRDT K+ P     K    SL  L++  LG  ++  +     ++  A MRLY+ +K +
Sbjct: 369 IRDTQKFKPFRSRVKSGRPSLKRLSEKILGIRVQQAEHC-SIQDAQAAMRLYVMVKRE 410

BLAST of CSPI05G28140 vs. ExPASy Swiss-Prot
Match: Q91560 (RNA exonuclease 4 OS=Xenopus laevis OX=8355 GN=rexo4 PE=2 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 7.3e-20
Identity = 61/177 (34.46%), Postives = 87/177 (49.15%), Query Frame = 0

Query: 64  KGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVR 123
           +G    VA+ C MVG G+DG  +I  RV +V+     ++  YV PT  +T+YR   +G+R
Sbjct: 229 EGLTRTVAMDCEMVGVGMDGEESILARVSIVNLFGKCVYDKYVKPTERVTDYRTAVSGIR 288

Query: 124 PEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPI 183
           PE ++   P K VQK++ E L               R R LVGH +   L  L +D+P  
Sbjct: 289 PEDVKKGEPFKVVQKEVSEIL---------------RGRTLVGHAVHNDLKILFLDHPKK 348

Query: 184 MIRDTAKYPPLMKTSKLSN-SLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMK 240
            IRDT KY P  +  K    SL  L +  L   ++ G+     ++  A MRLY   K
Sbjct: 349 AIRDTQKYKPFKQKVKSGRPSLKLLCEKILNVKVQTGEHC-SVQDAQAAMRLYTMEK 389

BLAST of CSPI05G28140 vs. ExPASy Swiss-Prot
Match: Q6DEW6 (RNA exonuclease 4 OS=Xenopus tropicalis OX=8364 GN=rexo4 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.0e-18
Identity = 57/177 (32.20%), Postives = 86/177 (48.59%), Query Frame = 0

Query: 64  KGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVR 123
           +G    VA+ C MVG G+DG  ++  RV +V+     ++  YV PT  +T+YR   +G+R
Sbjct: 223 EGLTRTVAMDCEMVGVGLDGEESMLARVSIVNLFGKCVYDKYVRPTERVTDYRTAVSGIR 282

Query: 124 PEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPI 183
           P+ +++    K VQ ++ E L               R R LVGH +   L  L +D+P  
Sbjct: 283 PDDIKNGEAFKDVQAEVAEIL---------------RGRTLVGHAVHNDLKILFLDHPKK 342

Query: 184 MIRDTAKYPPLMKTSKLSN-SLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMK 240
            IRDT KY P  +  K    SL  L +  L   ++ G+     ++  A MRLY   K
Sbjct: 343 AIRDTQKYKPFKEKVKSGRPSLKLLCEKILNVKVQTGEHC-SVQDAQAAMRLYTMEK 383

BLAST of CSPI05G28140 vs. ExPASy Swiss-Prot
Match: Q4WHF8 (RNA exonuclease 4 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=rex4 PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.4e-17
Identity = 64/205 (31.22%), Postives = 103/205 (50.24%), Query Frame = 0

Query: 70  VALSCTMVGAGID-GSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVRPEHLR 129
           VA+ C MVG G +  + +   RV +V+ N   ++ +YV P   IT++R   +G+ P+H+ 
Sbjct: 117 VAMDCEMVGVGPNPDNDSALARVSIVNFNGEQVYDSYVRPKEMITDWRTHVSGISPKHMA 176

Query: 130 DAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPIMIRDT 189
           +A  L+QVQK + E L                 RILVGH +   L +L + +P   IRDT
Sbjct: 177 EARSLEQVQKDVAEIL---------------DGRILVGHAVSNDLDALLLGHPKRDIRDT 236

Query: 190 AKYPPLMK-TSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVHKR-- 249
           +K+PP  K     S  L  L   +LG ++++G      E+  ATM LY R K    +   
Sbjct: 237 SKHPPYRKIAGGGSPRLKILASEFLGLNIQDGAH-SSVEDAKATMLLYRRDKEAFEREHL 296

Query: 250 EEYPLASDPQNKFNFANWKQLELEK 271
           +++P+      K N  + K+ + +K
Sbjct: 297 KKWPIRVVVDKKENGEDQKKKKKKK 305

BLAST of CSPI05G28140 vs. ExPASy TrEMBL
Match: A0A1S3C947 (RNA exonuclease 4 OS=Cucumis melo OX=3656 GN=LOC103498183 PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.9e-128
Identity = 228/297 (76.77%), Postives = 251/297 (84.51%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSHENR-----ARLGKT 63
           GPLPKQEC+NIF  RGCK CLAI +S     LHQE CQ  P+N     R      R G T
Sbjct: 60  GPLPKQECRNIFATRGCKFCLAILDSSYSQRLHQERCQFSPVNSGLLARFANLGIRDGST 119

Query: 64  --ESGGAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYR 123
             +SG  +G+  A+AL+C  VG G DGSL+IC +VC+VD+ E++IF +YV P +P+TNYR
Sbjct: 120 IIDSGRTRGSG-AIALACKYVGGGSDGSLDICAKVCLVDEGENVIFYSYVKPIIPVTNYR 179

Query: 124 YEFTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSL 183
           YEFTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGST +ARILVGHGLQEYLTSL
Sbjct: 180 YEFTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTRKARILVGHGLQEYLTSL 239

Query: 184 QIDYPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYM 243
           QIDYP IMIRDTAKYPPLMKTSKLSNSLH LTQTYLGY+MENGK+ EGYEECVATMRLY+
Sbjct: 240 QIDYPSIMIRDTAKYPPLMKTSKLSNSLHYLTQTYLGYNMENGKKSEGYEECVATMRLYL 299

Query: 244 RMKSQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDSTL 294
           +MKSQVHKREEYPLASDP NKFNFA WKQLELEKMTPQQLLQISRSDYYCWCLD+TL
Sbjct: 300 KMKSQVHKREEYPLASDPPNKFNFAIWKQLELEKMTPQQLLQISRSDYYCWCLDATL 355

BLAST of CSPI05G28140 vs. ExPASy TrEMBL
Match: A0A5A7T8A0 (RNA exonuclease 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G001310 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.5e-124
Identity = 227/308 (73.70%), Postives = 251/308 (81.49%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRP---INFSHENRARLGKT-- 63
           GPLPKQEC+NIF  RGCK CLAI +S     LHQE CQ  P     F++    R G T  
Sbjct: 32  GPLPKQECRNIFATRGCKFCLAILDSSYSQRLHQERCQFSPGLLARFANLG-IRDGSTII 91

Query: 64  ESGGAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYE 123
           +SG  +G+  A+AL+C  VG G DGSL+IC +VC+VD+ E++IF +YV P +P+TNYRYE
Sbjct: 92  DSGRTRGSG-AIALACKYVGGGSDGSLDICAKVCLVDEGENVIFYSYVKPIIPVTNYRYE 151

Query: 124 FTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQI 183
           FTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGST +ARILVGHGLQEYLTSLQI
Sbjct: 152 FTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTRKARILVGHGLQEYLTSLQI 211

Query: 184 DYPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLG-------------YSMENGKRLEGY 243
           DYP IMIRDTAKYPPLMKTSKLSNSLH LTQTYLG             Y+MENGK+ EGY
Sbjct: 212 DYPSIMIRDTAKYPPLMKTSKLSNSLHYLTQTYLGQVFHITLNSLIILYNMENGKKSEGY 271

Query: 244 EECVATMRLYMRMKSQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYY 294
           EECVATMRLY++MKSQVHKREEYPLASDP NKFNFA WKQLELEKMTPQQLLQISRSDYY
Sbjct: 272 EECVATMRLYLKMKSQVHKREEYPLASDPPNKFNFAIWKQLELEKMTPQQLLQISRSDYY 331

BLAST of CSPI05G28140 vs. ExPASy TrEMBL
Match: A0A6J1JNC8 (RNA exonuclease 4 OS=Cucurbita maxima OX=3661 GN=LOC111487420 PE=3 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 5.1e-117
Identity = 212/297 (71.38%), Postives = 240/297 (80.81%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSH-----ENRARLGK- 63
           GPLPKQEC NIF  RGCKICLAIFESP  L LHQE CQLR  NFS       N     K 
Sbjct: 60  GPLPKQECNNIFGTRGCKICLAIFESPHTLRLHQERCQLRSPNFSQIMARFRNMGICDKL 119

Query: 64  TESGGAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRY 123
           T   G    +EAVAL+C MVG G DGSL+IC RVC++D+ E++IFSTYVNPTLPITNYR 
Sbjct: 120 TSDNGRMRRHEAVALACRMVGGGSDGSLDICARVCLIDEKETIIFSTYVNPTLPITNYRL 179

Query: 124 EFTGVRPEHLRD-AMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSL 183
           E TGV PE+LRD AMPLKQVQKKIQ+ LCNGE MWKIRPG  G+ARILVGHGL ++L +L
Sbjct: 180 ESTGVGPEYLRDHAMPLKQVQKKIQDILCNGEPMWKIRPGVGGKARILVGHGLGDHLKTL 239

Query: 184 QIDYPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYM 243
           QI+YPP MIRDTA YPPLMKTSKL+NSLH LTQ YLGY ++NG + + YE+CVATMRLYM
Sbjct: 240 QIEYPPFMIRDTANYPPLMKTSKLTNSLHYLTQAYLGYDIQNGNK-DPYEDCVATMRLYM 299

Query: 244 RMKSQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDSTL 294
           RM++QVHK EEYPLASDPQNK NFA WKQ ELE+M+P+Q+L+ISRSDYYCWCLDS L
Sbjct: 300 RMRAQVHKIEEYPLASDPQNKNNFAIWKQFELERMSPEQMLEISRSDYYCWCLDSKL 355

BLAST of CSPI05G28140 vs. ExPASy TrEMBL
Match: A0A6J1HBP2 (RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111462538 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 7.4e-116
Identity = 210/297 (70.71%), Postives = 239/297 (80.47%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSH-----ENRARLGK- 63
           GP PKQEC N+F  RGCKICLAIFESP  L LHQE CQLR  N S       N     K 
Sbjct: 60  GPRPKQECNNVFVIRGCKICLAIFESPHTLRLHQERCQLRSPNSSQITTRFRNMGICDKL 119

Query: 64  TESGGAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRY 123
           T   G    +EAVAL+C MVG G DGSL+IC RVC++D+NE++IFSTYVNPTLPITNYR 
Sbjct: 120 TSDNGRMRRHEAVALACRMVGGGSDGSLDICARVCLIDENETIIFSTYVNPTLPITNYRL 179

Query: 124 EFTGVRPEHLRD-AMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSL 183
           E TGV PE++RD AMPLKQVQKKIQ+ LCNGE MWKIRPG  G+ARILVGHGL ++L SL
Sbjct: 180 ESTGVGPEYMRDRAMPLKQVQKKIQDILCNGEPMWKIRPGVGGKARILVGHGLGDHLKSL 239

Query: 184 QIDYPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYM 243
           QI+YPP MIRDTA YPPLMKTSKL+NSLH LTQ YLGY ++NG + + YE+CVATMRLYM
Sbjct: 240 QIEYPPFMIRDTANYPPLMKTSKLTNSLHYLTQAYLGYDIQNGSK-DPYEDCVATMRLYM 299

Query: 244 RMKSQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDSTL 294
           RM+SQVHK EEYPLASDPQNK NFA WKQ ELE+M+P+Q+L++SRSDYYCWCLDS L
Sbjct: 300 RMRSQVHKIEEYPLASDPQNKNNFAIWKQFELERMSPEQMLEVSRSDYYCWCLDSKL 355

BLAST of CSPI05G28140 vs. ExPASy TrEMBL
Match: A0A5E4EQL4 (RNA exonuclease 4 OS=Prunus dulcis OX=3755 GN=ALMOND_2B000672 PE=3 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 6.7e-109
Identity = 193/292 (66.10%), Postives = 236/292 (80.82%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSHENR-ARLG---KTE 63
           GPLPKQEC+NIF+ RGCK CLAI +SP  L +HQ+ CQL  +N     R A LG    TE
Sbjct: 63  GPLPKQECRNIFSTRGCKFCLAILDSPYALRVHQDRCQLSGVNAGLLGRFANLGIRDNTE 122

Query: 64  SGGAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEF 123
           +G  +G  + VAL+C MVG G DGSL++C +VC++D+ E++IF +YV P LP+TNYRYE 
Sbjct: 123 NGSTRG-TQVVALACKMVGGGSDGSLDLCAKVCLIDEYENIIFQSYVKPLLPVTNYRYET 182

Query: 124 TGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQID 183
           TG+RPE+LRDAMPL+QVQKKIQ+FLCNGE MWKIRP   G+ARILVGHGL   L SLQ++
Sbjct: 183 TGIRPEYLRDAMPLRQVQKKIQDFLCNGEPMWKIRPRG-GKARILVGHGLDHDLDSLQVE 242

Query: 184 YPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMK 243
           YP +MIRDTAKYPPLMKTSKLSNSL  LTQ YLGY ++ G + + YE+CVATM+LYMRM+
Sbjct: 243 YPQVMIRDTAKYPPLMKTSKLSNSLKYLTQAYLGYDIQTGIQ-DPYEDCVATMKLYMRMR 302

Query: 244 SQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDS 292
           SQVHK E YPLASDPQN+ NFA+W+Q ELE+M+P+Q+L+ SRSDYYCWCLDS
Sbjct: 303 SQVHKTEAYPLASDPQNRNNFASWRQNELERMSPEQMLEASRSDYYCWCLDS 351

BLAST of CSPI05G28140 vs. NCBI nr
Match: XP_011656271.2 (RNA exonuclease 4 [Cucumis sativus])

HSP 1 Score: 601.3 bits (1549), Expect = 4.5e-168
Identity = 288/290 (99.31%), Postives = 289/290 (99.66%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSHENRARLGKTESGGA 63
           GPLPKQECKNIFNARGCKICLA+FESPLQLNLHQESCQLRPINFSHENRARLGKTESGG 
Sbjct: 60  GPLPKQECKNIFNARGCKICLAMFESPLQLNLHQESCQLRPINFSHENRARLGKTESGGT 119

Query: 64  KGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVR 123
           KGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVR
Sbjct: 120 KGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVR 179

Query: 124 PEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPI 183
           PEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPI
Sbjct: 180 PEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPI 239

Query: 184 MIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVH 243
           MIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVH
Sbjct: 240 MIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVH 299

Query: 244 KREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDSTL 294
           KREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDSTL
Sbjct: 300 KREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDSTL 349

BLAST of CSPI05G28140 vs. NCBI nr
Match: KAE8648891.1 (hypothetical protein Csa_008012 [Cucumis sativus])

HSP 1 Score: 509.6 bits (1311), Expect = 1.8e-140
Identity = 249/265 (93.96%), Postives = 250/265 (94.34%), Query Frame = 0

Query: 29  SPLQLNLHQESCQLRPINFSHENRARLGKTESGGAKGNNEAVALSCTMVGAGIDGSLNIC 88
           +P   N       L  I  SHENRARLGKTESGG KGNNEAVALSCTMVGAGIDGSLNIC
Sbjct: 5   APFATNTSDTLNLLENILLSHENRARLGKTESGGTKGNNEAVALSCTMVGAGIDGSLNIC 64

Query: 89  VRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVRPEHLRDAMPLKQVQKKIQEFLCNGE 148
           VRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVRPEHLRDAMPLKQVQKKIQEFLCNGE
Sbjct: 65  VRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVRPEHLRDAMPLKQVQKKIQEFLCNGE 124

Query: 149 QMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPIMIRDTAKYPPLMKTSKLSNSLHCLT 208
           QMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPIMIRDTAKYPPLMKTSKLSNSLHCLT
Sbjct: 125 QMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPIMIRDTAKYPPLMKTSKLSNSLHCLT 184

Query: 209 QTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVHKREEYPLASDPQNKFNFANWKQLEL 268
           QTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVHKREEYPLASDPQNKFNFANWKQLEL
Sbjct: 185 QTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVHKREEYPLASDPQNKFNFANWKQLEL 244

Query: 269 EKMTPQQLLQISRSDYYCWCLDSTL 294
           EKMTPQQLLQISRSDYYCWCLDSTL
Sbjct: 245 EKMTPQQLLQISRSDYYCWCLDSTL 269

BLAST of CSPI05G28140 vs. NCBI nr
Match: XP_008458921.1 (PREDICTED: RNA exonuclease 4 [Cucumis melo])

HSP 1 Score: 468.0 bits (1203), Expect = 6.0e-128
Identity = 228/297 (76.77%), Postives = 251/297 (84.51%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSHENR-----ARLGKT 63
           GPLPKQEC+NIF  RGCK CLAI +S     LHQE CQ  P+N     R      R G T
Sbjct: 60  GPLPKQECRNIFATRGCKFCLAILDSSYSQRLHQERCQFSPVNSGLLARFANLGIRDGST 119

Query: 64  --ESGGAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYR 123
             +SG  +G+  A+AL+C  VG G DGSL+IC +VC+VD+ E++IF +YV P +P+TNYR
Sbjct: 120 IIDSGRTRGSG-AIALACKYVGGGSDGSLDICAKVCLVDEGENVIFYSYVKPIIPVTNYR 179

Query: 124 YEFTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSL 183
           YEFTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGST +ARILVGHGLQEYLTSL
Sbjct: 180 YEFTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTRKARILVGHGLQEYLTSL 239

Query: 184 QIDYPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYM 243
           QIDYP IMIRDTAKYPPLMKTSKLSNSLH LTQTYLGY+MENGK+ EGYEECVATMRLY+
Sbjct: 240 QIDYPSIMIRDTAKYPPLMKTSKLSNSLHYLTQTYLGYNMENGKKSEGYEECVATMRLYL 299

Query: 244 RMKSQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDSTL 294
           +MKSQVHKREEYPLASDP NKFNFA WKQLELEKMTPQQLLQISRSDYYCWCLD+TL
Sbjct: 300 KMKSQVHKREEYPLASDPPNKFNFAIWKQLELEKMTPQQLLQISRSDYYCWCLDATL 355

BLAST of CSPI05G28140 vs. NCBI nr
Match: KAA0037519.1 (RNA exonuclease 4 [Cucumis melo var. makuwa])

HSP 1 Score: 454.9 bits (1169), Expect = 5.2e-124
Identity = 227/308 (73.70%), Postives = 251/308 (81.49%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRP---INFSHENRARLGKT-- 63
           GPLPKQEC+NIF  RGCK CLAI +S     LHQE CQ  P     F++    R G T  
Sbjct: 32  GPLPKQECRNIFATRGCKFCLAILDSSYSQRLHQERCQFSPGLLARFANLG-IRDGSTII 91

Query: 64  ESGGAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYE 123
           +SG  +G+  A+AL+C  VG G DGSL+IC +VC+VD+ E++IF +YV P +P+TNYRYE
Sbjct: 92  DSGRTRGSG-AIALACKYVGGGSDGSLDICAKVCLVDEGENVIFYSYVKPIIPVTNYRYE 151

Query: 124 FTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQI 183
           FTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGST +ARILVGHGLQEYLTSLQI
Sbjct: 152 FTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTRKARILVGHGLQEYLTSLQI 211

Query: 184 DYPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLG-------------YSMENGKRLEGY 243
           DYP IMIRDTAKYPPLMKTSKLSNSLH LTQTYLG             Y+MENGK+ EGY
Sbjct: 212 DYPSIMIRDTAKYPPLMKTSKLSNSLHYLTQTYLGQVFHITLNSLIILYNMENGKKSEGY 271

Query: 244 EECVATMRLYMRMKSQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYY 294
           EECVATMRLY++MKSQVHKREEYPLASDP NKFNFA WKQLELEKMTPQQLLQISRSDYY
Sbjct: 272 EECVATMRLYLKMKSQVHKREEYPLASDPPNKFNFAIWKQLELEKMTPQQLLQISRSDYY 331

BLAST of CSPI05G28140 vs. NCBI nr
Match: XP_022990586.1 (RNA exonuclease 4-like [Cucurbita maxima])

HSP 1 Score: 430.6 bits (1106), Expect = 1.1e-116
Identity = 212/297 (71.38%), Postives = 240/297 (80.81%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSH-----ENRARLGK- 63
           GPLPKQEC NIF  RGCKICLAIFESP  L LHQE CQLR  NFS       N     K 
Sbjct: 60  GPLPKQECNNIFGTRGCKICLAIFESPHTLRLHQERCQLRSPNFSQIMARFRNMGICDKL 119

Query: 64  TESGGAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRY 123
           T   G    +EAVAL+C MVG G DGSL+IC RVC++D+ E++IFSTYVNPTLPITNYR 
Sbjct: 120 TSDNGRMRRHEAVALACRMVGGGSDGSLDICARVCLIDEKETIIFSTYVNPTLPITNYRL 179

Query: 124 EFTGVRPEHLRD-AMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSL 183
           E TGV PE+LRD AMPLKQVQKKIQ+ LCNGE MWKIRPG  G+ARILVGHGL ++L +L
Sbjct: 180 ESTGVGPEYLRDHAMPLKQVQKKIQDILCNGEPMWKIRPGVGGKARILVGHGLGDHLKTL 239

Query: 184 QIDYPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYM 243
           QI+YPP MIRDTA YPPLMKTSKL+NSLH LTQ YLGY ++NG + + YE+CVATMRLYM
Sbjct: 240 QIEYPPFMIRDTANYPPLMKTSKLTNSLHYLTQAYLGYDIQNGNK-DPYEDCVATMRLYM 299

Query: 244 RMKSQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDSTL 294
           RM++QVHK EEYPLASDPQNK NFA WKQ ELE+M+P+Q+L+ISRSDYYCWCLDS L
Sbjct: 300 RMRAQVHKIEEYPLASDPQNKNNFAIWKQFELERMSPEQMLEISRSDYYCWCLDSKL 355

BLAST of CSPI05G28140 vs. TAIR 10
Match: AT5G40310.1 (Exonuclease family protein )

HSP 1 Score: 368.6 bits (945), Expect = 4.6e-102
Identity = 181/293 (61.77%), Postives = 219/293 (74.74%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSHENR-ARLG----KT 63
           GPLPKQECKNIF+ RGC+ CL I ESP    +HQE CQL  +      R A LG     T
Sbjct: 56  GPLPKQECKNIFSIRGCRFCLTILESPNARRIHQERCQLSNVTSGLMIRMAALGLRNNST 115

Query: 64  ESGGAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYE 123
               +  +   VALSC MVG G DGSL++C RVC+ D++E+++F TYV PT+P+TNYRYE
Sbjct: 116 IDYTSSRSPRVVALSCKMVGGGSDGSLDLCARVCITDESENVVFHTYVKPTIPVTNYRYE 175

Query: 124 FTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQI 183
            TG+RPE+LRDAM LK  Q+K+QEFLCNGE MWKIRP   G+ARILVGHGL  +L SLQ+
Sbjct: 176 MTGIRPENLRDAMRLKHAQRKVQEFLCNGEPMWKIRP-RNGKARILVGHGLDNHLDSLQL 235

Query: 184 DYPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRM 243
           +Y   MIRDTA+YPPLMK+SKLSNSL  LTQ YLGY +  G + + YE+CVATMRLY RM
Sbjct: 236 EYSSSMIRDTAEYPPLMKSSKLSNSLKYLTQAYLGYDIHVGIQ-DPYEDCVATMRLYTRM 295

Query: 244 KSQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDS 292
           + Q H+ E YPLASD QN  NFA W+Q ELE+M+P++LL +SRSDYYCWCLDS
Sbjct: 296 RYQKHRAEAYPLASDTQNHNNFAAWRQNELERMSPEELLDLSRSDYYCWCLDS 346

BLAST of CSPI05G28140 vs. TAIR 10
Match: AT3G27970.1 (Exonuclease family protein )

HSP 1 Score: 365.5 bits (937), Expect = 3.9e-101
Identity = 179/293 (61.09%), Postives = 217/293 (74.06%), Query Frame = 0

Query: 4   GPLPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSHENR-ARLGKTESG- 63
           GPLPKQECKNIF+ RGC+ C+ I ESP    +HQE CQ   +N     R A LG  +   
Sbjct: 65  GPLPKQECKNIFSLRGCRFCMTILESPNSRRIHQERCQFSSVNSGLTTRMAALGLRDKAM 124

Query: 64  ---GAKGNNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYE 123
               +  +   VALSC MVG G DGSL++C RVC+ D+++++IF TYV P++ +T+YRYE
Sbjct: 125 IDYTSSRSPRVVALSCKMVGGGSDGSLDLCARVCITDESDNVIFHTYVKPSMAVTSYRYE 184

Query: 124 FTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQI 183
            TG+RPE+LRDAMPLKQVQ+KIQEFLCNGE MWKIRP   G+ARILVGHGL   L  LQ+
Sbjct: 185 TTGIRPENLRDAMPLKQVQRKIQEFLCNGEPMWKIRPRG-GKARILVGHGLDHDLDRLQL 244

Query: 184 DYPPIMIRDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRM 243
           +YP  MIRDTAKYPPLMKTSKLSNSL  LTQ YLGY +  G + + YE+CVATMRLY RM
Sbjct: 245 EYPSSMIRDTAKYPPLMKTSKLSNSLKYLTQAYLGYDVHFGIQ-DPYEDCVATMRLYTRM 304

Query: 244 KSQVHKREEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLDS 292
           + Q HK E YPLA+D QN+ N   W+Q E E+M+P ++L ISRSDYYCWCLDS
Sbjct: 305 RYQKHKIEAYPLAADAQNRSNQVAWRQSEAERMSPDEMLSISRSDYYCWCLDS 355

BLAST of CSPI05G28140 vs. TAIR 10
Match: AT2G48100.1 (Exonuclease family protein )

HSP 1 Score: 233.8 bits (595), Expect = 1.8e-61
Identity = 124/285 (43.51%), Postives = 175/285 (61.40%), Query Frame = 0

Query: 6   LPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSHENRARLGKTESGGAKG 65
           L K  CK IF  RGC +CL IFE    L  H+  C L P      +  R   +   G++ 
Sbjct: 71  LSKGNCKAIFTKRGCTLCLQIFEEAFALAEHKNKCHLSPPRPLGTSTQRNPSSSLAGSR- 130

Query: 66  NNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVRPE 125
             +A+AL C MVG G DG+++ C  VC+VD +E++IFST+V P LP+T+YR+E TG+  E
Sbjct: 131 -LKAMALDCEMVGGGADGTIDQCASVCLVDDDENVIFSTHVQPLLPVTDYRHEITGLTKE 190

Query: 126 HLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPIMI 185
            L+D MPL+ V++++  FLC G+          GR  +LVGH L+  ++ L+++YP  ++
Sbjct: 191 DLKDGMPLEHVRERVFSFLCGGQN------DGAGRL-LLVGHDLRHDMSCLKLEYPSHLL 250

Query: 186 RDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVHKR 245
           RDTAKY PLMKT+ +S SL  LT++YLGY ++ GK  E YE+CV+ MRLY RM+ Q H  
Sbjct: 251 RDTAKYVPLMKTNLVSQSLKYLTKSYLGYKIQCGKH-EVYEDCVSAMRLYKRMRDQEH-- 310

Query: 246 EEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLD 291
               + S         + KQ +LEKM  ++L Q S S+Y CWCLD
Sbjct: 311 ----VCSGKAEGNGLNSRKQSDLEKMNAEELYQKSTSEYRCWCLD 339

BLAST of CSPI05G28140 vs. TAIR 10
Match: AT2G48100.2 (Exonuclease family protein )

HSP 1 Score: 233.8 bits (595), Expect = 1.8e-61
Identity = 124/285 (43.51%), Postives = 175/285 (61.40%), Query Frame = 0

Query: 6   LPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSHENRARLGKTESGGAKG 65
           L K  CK IF  RGC +CL IFE    L  H+  C L P      +  R   +   G++ 
Sbjct: 71  LSKGNCKAIFTKRGCTLCLQIFEEAFALAEHKNKCHLSPPRPLGTSTQRNPSSSLAGSR- 130

Query: 66  NNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVRPE 125
             +A+AL C MVG G DG+++ C  VC+VD +E++IFST+V P LP+T+YR+E TG+  E
Sbjct: 131 -LKAMALDCEMVGGGADGTIDQCASVCLVDDDENVIFSTHVQPLLPVTDYRHEITGLTKE 190

Query: 126 HLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPIMI 185
            L+D MPL+ V++++  FLC G+          GR  +LVGH L+  ++ L+++YP  ++
Sbjct: 191 DLKDGMPLEHVRERVFSFLCGGQN------DGAGRL-LLVGHDLRHDMSCLKLEYPSHLL 250

Query: 186 RDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVHKR 245
           RDTAKY PLMKT+ +S SL  LT++YLGY ++ GK  E YE+CV+ MRLY RM+ Q H  
Sbjct: 251 RDTAKYVPLMKTNLVSQSLKYLTKSYLGYKIQCGKH-EVYEDCVSAMRLYKRMRDQEH-- 310

Query: 246 EEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLD 291
               + S         + KQ +LEKM  ++L Q S S+Y CWCLD
Sbjct: 311 ----VCSGKAEGNGLNSRKQSDLEKMNAEELYQKSTSEYRCWCLD 339

BLAST of CSPI05G28140 vs. TAIR 10
Match: AT2G48100.3 (Exonuclease family protein )

HSP 1 Score: 233.8 bits (595), Expect = 1.8e-61
Identity = 124/285 (43.51%), Postives = 175/285 (61.40%), Query Frame = 0

Query: 6   LPKQECKNIFNARGCKICLAIFESPLQLNLHQESCQLRPINFSHENRARLGKTESGGAKG 65
           L K  CK IF  RGC +CL IFE    L  H+  C L P      +  R   +   G++ 
Sbjct: 71  LSKGNCKAIFTKRGCTLCLQIFEEAFALAEHKNKCHLSPPRPLGTSTQRNPSSSLAGSR- 130

Query: 66  NNEAVALSCTMVGAGIDGSLNICVRVCVVDQNESLIFSTYVNPTLPITNYRYEFTGVRPE 125
             +A+AL C MVG G DG+++ C  VC+VD +E++IFST+V P LP+T+YR+E TG+  E
Sbjct: 131 -LKAMALDCEMVGGGADGTIDQCASVCLVDDDENVIFSTHVQPLLPVTDYRHEITGLTKE 190

Query: 126 HLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTGRARILVGHGLQEYLTSLQIDYPPIMI 185
            L+D MPL+ V++++  FLC G+          GR  +LVGH L+  ++ L+++YP  ++
Sbjct: 191 DLKDGMPLEHVRERVFSFLCGGQN------DGAGRL-LLVGHDLRHDMSCLKLEYPSHLL 250

Query: 186 RDTAKYPPLMKTSKLSNSLHCLTQTYLGYSMENGKRLEGYEECVATMRLYMRMKSQVHKR 245
           RDTAKY PLMKT+ +S SL  LT++YLGY ++ GK  E YE+CV+ MRLY RM+ Q H  
Sbjct: 251 RDTAKYVPLMKTNLVSQSLKYLTKSYLGYKIQCGKH-EVYEDCVSAMRLYKRMRDQEH-- 310

Query: 246 EEYPLASDPQNKFNFANWKQLELEKMTPQQLLQISRSDYYCWCLD 291
               + S         + KQ +LEKM  ++L Q S S+Y CWCLD
Sbjct: 311 ----VCSGKAEGNGLNSRKQSDLEKMNAEELYQKSTSEYRCWCLD 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9GZR22.7e-2234.36RNA exonuclease 4 OS=Homo sapiens OX=9606 GN=REXO4 PE=1 SV=2[more]
Q6PAQ42.5e-2034.27RNA exonuclease 4 OS=Mus musculus OX=10090 GN=Rexo4 PE=2 SV=2[more]
Q915607.3e-2034.46RNA exonuclease 4 OS=Xenopus laevis OX=8355 GN=rexo4 PE=2 SV=2[more]
Q6DEW64.0e-1832.20RNA exonuclease 4 OS=Xenopus tropicalis OX=8364 GN=rexo4 PE=2 SV=1[more]
Q4WHF84.4e-1731.22RNA exonuclease 4 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 10... [more]
Match NameE-valueIdentityDescription
A0A1S3C9472.9e-12876.77RNA exonuclease 4 OS=Cucumis melo OX=3656 GN=LOC103498183 PE=3 SV=1[more]
A0A5A7T8A02.5e-12473.70RNA exonuclease 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G00... [more]
A0A6J1JNC85.1e-11771.38RNA exonuclease 4 OS=Cucurbita maxima OX=3661 GN=LOC111487420 PE=3 SV=1[more]
A0A6J1HBP27.4e-11670.71RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111462538 PE=3 SV=1[more]
A0A5E4EQL46.7e-10966.10RNA exonuclease 4 OS=Prunus dulcis OX=3755 GN=ALMOND_2B000672 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011656271.24.5e-16899.31RNA exonuclease 4 [Cucumis sativus][more]
KAE8648891.11.8e-14093.96hypothetical protein Csa_008012 [Cucumis sativus][more]
XP_008458921.16.0e-12876.77PREDICTED: RNA exonuclease 4 [Cucumis melo][more]
KAA0037519.15.2e-12473.70RNA exonuclease 4 [Cucumis melo var. makuwa][more]
XP_022990586.11.1e-11671.38RNA exonuclease 4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G40310.14.6e-10261.77Exonuclease family protein [more]
AT3G27970.13.9e-10161.09Exonuclease family protein [more]
AT2G48100.11.8e-6143.51Exonuclease family protein [more]
AT2G48100.21.8e-6143.51Exonuclease family protein [more]
AT2G48100.31.8e-6143.51Exonuclease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 68..244
e-value: 2.9E-14
score: 63.4
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 85..190
e-value: 1.8E-7
score: 31.8
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 64..255
e-value: 5.4E-36
score: 125.8
NoneNo IPR availablePANTHERPTHR12801RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATEDcoord: 4..291
NoneNo IPR availablePANTHERPTHR12801:SF122OS02G0709000 PROTEINcoord: 4..291
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 71..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G28140.1CSPI05G28140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006364 rRNA processing
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding