CSPI05G27590 (gene) Cucumber (PI 183967) v1

BLAST of CSPI05G27590 vs. ExPASy Swiss-Prot
Match: Q9M1I2 (Protein NRT1/ PTR FAMILY 5.4 OS=Arabidopsis thaliana OX=3702 GN=NPF5.4 PE=2 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 7.7e-178
Identity = 297/551 (53.90%), Postives = 413/551 (74.95%), Query Frame = 0

Query: 32  RPGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFHEPLGVAAKQVNNWVGVSAVFP 91
           R  GGW AA+FII VE+AE+FA  GL+SNLI + T    +    AAK +N W+GVS +FP
Sbjct: 11  RTKGGWNAALFIIVVEIAERFAFYGLASNLITFLTNELGQSTATAAKNINTWIGVSCMFP 70

Query: 92  LLGAFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVGDNQRKAVFFLGLYILSVGQGG 151
           +LGAF+ADS+LGRFKT+++ S I+ +G+++L +S TVV    R+ VFF+ LY+++VG+GG
Sbjct: 71  ILGAFLADSILGRFKTVLLTSFIYLLGIVMLPLSVTVVARRMREKVFFMALYVMAVGEGG 130

Query: 152 HRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGSTFAVFVVIYVQDNIGWGLSFG 211
           H+PCV TFAADQF E + EE+  K+SFFN+WY+ +V  S+ AV  +I++Q+ + W L F 
Sbjct: 131 HKPCVMTFAADQFGEANAEEKAAKTSFFNYWYMAIVLASSIAVLALIFIQERVSWSLGFS 190

Query: 212 ILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVAAARKWRVDETRNGWRVCYEED 271
           I+AG +  AI++FL G+ KYR+QVPVGSP TR+AQV+VAA +KWR+  TR+ + +CYEE+
Sbjct: 191 IIAGSVVIAIVIFLIGIPKYRKQVPVGSPFTRVAQVMVAALKKWRLSSTRHHYGLCYEEE 250

Query: 272 NRAKNDAEGEHNLMTLARTNQFRILDKATLIDKEDEARKKRDPWRLSTVEEVEEVKLVVR 331
           +  K ++   + +  LARTNQFR LDKAT+ID+ D   K R+PWRL TV +VEEVKL++R
Sbjct: 251 DEHKLESTNSNQVYLLARTNQFRFLDKATIIDEIDH-NKNRNPWRLCTVNQVEEVKLILR 310

Query: 332 LIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQLPPASLQGVVGLTILLTVLFYD 391
           LIP+W+S +MF     Q++TFF KQGS M R++G HF +PPA+ Q +VG+TIL+ +  YD
Sbjct: 311 LIPIWISLIMFCATLTQLNTFFLKQGSMMDRTIGNHFTIPPAAFQSIVGVTILILIPLYD 370

Query: 392 RVFVPAARNFTGHHSGITVLQRIGMGLFISIFTMGVSALVEAKRVTIAAEHGLSDTPKAT 451
           RVFVP  R  T HHSGIT LQRIG+GLF++ F M +  LVEAKR+ +A +HGL D+PK  
Sbjct: 371 RVFVPMVRKITNHHSGITSLQRIGVGLFVATFNMVICGLVEAKRLKVARDHGLIDSPKEV 430

Query: 452 VPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQMPEFMRSLGAAAYISIIGVGNFLSSAI 511
           VPM+  WL+PQY+L G+ D F I+G+QELFYDQMPE MRS+GAA +IS++GVG+F+S+ I
Sbjct: 431 VPMSSLWLLPQYILVGIGDVFTIVGMQELFYDQMPETMRSIGAAIFISVVGVGSFVSTGI 490

Query: 512 ISVVQAGS---GGRWLEDNLNRSNLHYFYWVLAALSALNLCGYVWIANGFVYKRAGGNRR 571
           IS VQ  S   G  WL +NLNR++L Y+YW++A+L+A++LC Y++IAN F+YK+      
Sbjct: 491 ISTVQTISKSHGEEWLVNNLNRAHLDYYYWIIASLNAVSLCFYLFIANHFLYKK------ 550

Query: 572 SISGDDDDVKN 580
            +   DDDV++
Sbjct: 551 -LQDKDDDVES 553

BLAST of CSPI05G27590 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.5e-133
Identity = 255/555 (45.95%), Postives = 358/555 (64.50%), Query Frame = 0

Query: 27  NSPTVR-PGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFHEPLGVAAKQVNNWVG 86
           N P V+   GGW++A FII VEVAE+FA  G+SSNLI Y T    +    AA  VN W G
Sbjct: 23  NKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQSTAAAAANVNAWSG 82

Query: 87  VSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVGDNQ------------ 146
            +++ PLLGAFVADS LGRF+TI+ AS ++ +G+ VLT+SA +  D +            
Sbjct: 83  TASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSDCKVSNLLSSCSPRF 142

Query: 147 RKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGSTFA 206
           +   FF  LY++++ QGGH+PCVQ F ADQFDE+ PEE K KSSFFNWWY G+  G+   
Sbjct: 143 QVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSFFNWWYFGMCFGTLTT 202

Query: 207 VFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQV--PVGSPLTRIAQVVVAA 266
           ++V+ Y+QDN+ W L FGI    +  A+++ L G   YR  +     SP  RI  V VAA
Sbjct: 203 LWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQSPFVRIGNVYVAA 262

Query: 267 ARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKATLIDKEDEARKK 326
            + W V             D  A   AE    L++ + + QF  L+KA L+ K       
Sbjct: 263 VKNWSVSAL----------DVAA---AEERLGLVSCSSSQQFSFLNKA-LVAKNGSC--- 322

Query: 327 RDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQLP 386
                  +++E+EE K V+RL P+W++CL++AVV AQ  TFFTKQG+TM RS+ P +++ 
Sbjct: 323 -------SIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKIS 382

Query: 387 PASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFISIFTMGVSALV 446
           PA+LQ  + L+I++ +  YDRV +P AR+FT    GIT+LQRIG G+F+S   M V+ALV
Sbjct: 383 PATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAALV 442

Query: 447 EAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQMPEFMRS 506
           E KR+  AA++GL D+P ATVPM++WWL+PQY+L G++D FA++GLQE FYDQ+P  +RS
Sbjct: 443 EMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELRS 502

Query: 507 LGAAAYISIIGVGNFLSSAIISVVQAGSG----GRWLEDNLNRSNLHYFYWVLAALSALN 563
           +G A Y+SI G+GNFLSS +IS+++  +       W  +NLN+++L YFYW+LA LS + 
Sbjct: 503 VGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFIG 553

BLAST of CSPI05G27590 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 452.6 bits (1163), Expect = 6.9e-126
Identity = 244/549 (44.44%), Postives = 349/549 (63.57%), Query Frame = 0

Query: 35  GGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLG 94
           G W+AA FII VEVAE+FA+ G+ SNLI Y T    +   VAA  VN W G+S + PLLG
Sbjct: 31  GRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQSTAVAAANVNAWSGISTILPLLG 90

Query: 95  AFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVGDNQR---------KAVFFLGLYIL 154
           AFVAD+ LGR+ TIIIAS I+ +G+  LT+SA ++ +N            A+FF  LY++
Sbjct: 91  AFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNNTEVTSSPSSFLNALFFFSLYLV 150

Query: 155 SVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGSTFAVFVVIYVQDNIG 214
           ++GQ GH+PCVQ F ADQFDE++P+E   +SSFFNWWY+ +  G   A+ VV+Y+Q+N+ 
Sbjct: 151 AIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVS 210

Query: 215 WGLSFGILAGVLAAAIILFLAGVKKYR----RQVPVGSPLTRIAQVVVAAARKWRVDETR 274
           W L FGI    +  +++LF+ G K YR    RQ    +P TRI +V   A +  R++ + 
Sbjct: 211 WALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSS- 270

Query: 275 NGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKATLI----DKEDEARKKRDPWRL 334
           +  +V   E NR++   E            +   L+KA L+    D+ + A K RD    
Sbjct: 271 DLCKVELIEANRSQESPE------------ELSFLNKALLVPNDSDEGEVACKSRD---- 330

Query: 335 STVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQLPPASLQG 394
                VE+   +VRLIPVW++ L +A+  AQ  TFFTKQG TM R++ P  ++PPASLQ 
Sbjct: 331 -----VEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPASLQV 390

Query: 395 VVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFISIFTMGVSALVEAKRVT 454
           ++ ++I+L V  YDRV VP  R+ T    GIT L+RIG G+ ++  TM V+ALVE+KR+ 
Sbjct: 391 LISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESKRLE 450

Query: 455 IAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQMPEFMRSLGAAAY 514
            A E+GL D PK T+PM+IWWL PQYML G++D   ++G+QE FY Q+P  +RSLG A Y
Sbjct: 451 TAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGLAIY 510

Query: 515 ISIIGVGNFLSSAIISVVQAGSGG----RWLEDNLNRSNLHYFYWVLAALSALNLCGYVW 563
           +S +GVG+ LSS +I ++   +GG     W   NLNR++L YFYW+LA +SA+    +++
Sbjct: 511 LSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFTFLF 557

BLAST of CSPI05G27590 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 442.6 bits (1137), Expect = 7.1e-123
Identity = 234/545 (42.94%), Postives = 339/545 (62.20%), Query Frame = 0

Query: 35  GGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLG 94
           G W+AA+FII VEVAE+FA  G+ SNLI Y T    E   VAA  VN W G++ + P+LG
Sbjct: 31  GRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAVAAANVNAWSGIATLLPVLG 90

Query: 95  AFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVGDNQR---------KAVFFLGLYIL 154
           AFVAD+ LGR++TIII+SLI+ +G+  LT+SA ++ +              +FF  LY++
Sbjct: 91  AFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEVTSSTSSFLNVLFFFSLYLV 150

Query: 155 SVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGSTFAVFVVIYVQDNIG 214
           ++GQ GH+PCVQ F ADQFDE+  +E+  +SSFFNWWY+ L  G  FA+ VV+Y+Q+   
Sbjct: 151 AIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWYLSLSAGICFAILVVVYIQEEFS 210

Query: 215 WGLSFGILAGVLAAAIILFLAGVKKY----RRQVPVGSPLTRIAQVVVAAARKWRVDETR 274
           W   FGI    +  +++LF++G + Y    RR     +P TRI +V   A +  R+  + 
Sbjct: 211 WAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRIGRVFFVALKNQRLSSS- 270

Query: 275 NGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKATLIDKEDEARKKRDPWRLSTVE 334
               +C  E             L       +    +KA L+  +    +       S   
Sbjct: 271 ---DLCKVE-------------LEANTSPEKQSFFNKALLVPNDSSQGE-----NASKSS 330

Query: 335 EVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQLPPASLQGVVGL 394
           +VE+   ++RLIPVW + L +A+  AQ  TFFTKQG TM R++ P  ++PPASLQ  +G+
Sbjct: 331 DVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASLQVFIGI 390

Query: 395 TILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFISIFTMGVSALVEAKRVTIAAE 454
           +I+L V  YDRVFVP AR  T    GIT L+RIG G+ +S  TM ++ALVE KR+  A E
Sbjct: 391 SIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRLETAKE 450

Query: 455 HGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQMPEFMRSLGAAAYISII 514
           HGL D P+AT+PM+IWWLIPQY+L G++D + ++G+QE FY Q+P  +RS+G A Y+S +
Sbjct: 451 HGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLALYLSAL 510

Query: 515 GVGNFLSSAIISVVQAGSGG----RWLEDNLNRSNLHYFYWVLAALSALNLCGYVWIANG 563
           GVG+ LSS +IS++   +GG     W   NLNR++L YFYW+LA +SA+    +++I+  
Sbjct: 511 GVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGFFTFLFISKS 553

BLAST of CSPI05G27590 vs. ExPASy Swiss-Prot
Match: Q9M331 (Protein NRT1/ PTR FAMILY 5.7 OS=Arabidopsis thaliana OX=3702 GN=NPF5.7 PE=2 SV=2)

HSP 1 Score: 428.7 bits (1101), Expect = 1.1e-118
Identity = 231/570 (40.53%), Postives = 354/570 (62.11%), Query Frame = 0

Query: 35  GGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLG 94
           G W+AA+FII +E +E+ +  G+S+NL++Y TT+ H+ L +A K  N W GV+ + PLLG
Sbjct: 39  GAWRAALFIIGIEFSERLSYFGISTNLVVYLTTILHQDLKMAVKNTNYWSGVTTLMPLLG 98

Query: 95  AFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVG---------DNQRKA---VFFLGL 154
            FVAD+ LGR+ T+++A+ I+ +G+++LT+S  + G            RKA    FF+ +
Sbjct: 99  GFVADAYLGRYGTVLLATTIYLMGLILLTLSWFIPGLKACHEDMCVEPRKAHEIAFFIAI 158

Query: 155 YILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGSTFAVFVVIYVQD 214
           Y++S+G GGH+P +++F ADQF++  PEERK K S+FNWW  GL  G   AV V++Y++D
Sbjct: 159 YLISIGTGGHKPSLESFGADQFEDGHPEERKMKMSYFNWWNAGLCAGILTAVTVIVYIED 218

Query: 215 NIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVAAARKWRVDETRN 274
            IGWG++  IL  V+A +  +F  G   YR + P GSPLT + QV VAA  K  +     
Sbjct: 219 RIGWGVASIILTIVMATSFFIFRIGKPFYRYRAPSGSPLTPMLQVFVAAIAKRNLP---- 278

Query: 275 GWRVCYEEDNRAKNDAEGEHNL-MTLARTNQFRILDKATLIDKEDEARK--KRDPWRLST 334
               C  + +        E+     L+ +   + LDKA +I+  +E  K  K+ PWRL+T
Sbjct: 279 ----CPSDSSLLHELTNEEYTKGRLLSSSKNLKFLDKAAVIEDRNENTKAEKQSPWRLAT 338

Query: 335 VEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSV-GPHFQLPPASLQGV 394
           V +VEEVKL++ +IP+W   L F V   Q  T F KQ   M R + G  F +PPASL  +
Sbjct: 339 VTKVEEVKLLINMIPIWFFTLAFGVCATQSSTLFIKQAIIMDRHITGTSFIVPPASLFSL 398

Query: 395 VGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFISIFTMGVSALVEAKRVTI 454
           + L+I++TV  Y+++ VP  R  TG+  GI++LQRIG+G+  S+F M ++AL+E KR+  
Sbjct: 399 IALSIIITVTIYEKLLVPLLRRATGNERGISILQRIGVGMVFSLFAMIIAALIEKKRLDY 458

Query: 455 AAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQMPEFMRSLGAAAYI 514
           A EH ++     T+ ++  WL PQ+++ GV+DAF ++GLQE FYDQ+P+ MRSLG A Y+
Sbjct: 459 AKEHHMN----KTMTLSAIWLAPQFLVLGVADAFTLVGLQEYFYDQVPDSMRSLGIAFYL 518

Query: 515 SIIGVGNFLSSAIISV----VQAGSGGRWLEDNLNRSNLHYFYWVLAALSALNLCGYVWI 574
           S++G  +F+++ +I+V     +  SG  W   +LN S L  FYW+LAAL+A N+C +V +
Sbjct: 519 SVLGAASFVNNLLITVSDHLAEEISGKGWFGKDLNSSRLDRFYWMLAALTAANICCFVIV 578

Query: 575 ANGFVYKRAGGNRRSISGDDDDVKNSNNIN 585
           A  + YK    +   ++   DDV+ +   N
Sbjct: 579 AMRYTYKTVQPSLAVVADGGDDVETATGTN 596

BLAST of CSPI05G27590 vs. ExPASy TrEMBL
Match: A0A5D3CDG7 (Protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003090 PE=3 SV=1)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 567/592 (95.78%), Postives = 580/592 (97.97%), Query Frame = 0

Query: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60
           MEKQKSPNNVPIINLPNKFPDHPTASN+PTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN
Sbjct: 1   MEKQKSPNNVPIINLPNKFPDHPTASNTPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60

Query: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120
           LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASL+FF GM+
Sbjct: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMV 120

Query: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180
           VLTVSATVVGD+ RKAVFFLGLYILSVGQGGHRPCVQTFAADQF+E +PEERKKKSSFFN
Sbjct: 121 VLTVSATVVGDDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFN 180

Query: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240
           WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP
Sbjct: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKAT 300
           LTRIAQVVVAAARKW VDETR+ WRVCYEEDN AKN+ EG+HNLMTLARTNQFRILDKAT
Sbjct: 241 LTRIAQVVVAAARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFRILDKAT 300

Query: 301 LIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 360
           LIDKEDEARKKRDPWRLSTV EVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM
Sbjct: 301 LIDKEDEARKKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 360

Query: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 420
           LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI
Sbjct: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 420

Query: 421 SIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQEL 480
           SI TMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAI+GLQEL
Sbjct: 421 SILTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQEL 480

Query: 481 FYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWVL 540
           FYDQMP+FMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWL+DNLNRSNLHYFYWVL
Sbjct: 481 FYDQMPQFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLDDNLNRSNLHYFYWVL 540

Query: 541 AALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593
           AALSALNLCGYVWIANGFVYKR GGN RSI+GDD DVKNSNNINGCYGDDMI
Sbjct: 541 AALSALNLCGYVWIANGFVYKRVGGN-RSINGDDGDVKNSNNINGCYGDDMI 591

BLAST of CSPI05G27590 vs. ExPASy TrEMBL
Match: A0A1S3BFS1 (protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo OX=3656 GN=LOC103489513 PE=3 SV=1)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 567/592 (95.78%), Postives = 580/592 (97.97%), Query Frame = 0

Query: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60
           MEKQKSPNNVPIINLPNKFPDHPTASN+PTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN
Sbjct: 1   MEKQKSPNNVPIINLPNKFPDHPTASNTPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60

Query: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120
           LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASL+FF GM+
Sbjct: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMV 120

Query: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180
           VLTVSATVVGD+ RKAVFFLGLYILSVGQGGHRPCVQTFAADQF+E +PEERKKKSSFFN
Sbjct: 121 VLTVSATVVGDDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFN 180

Query: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240
           WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP
Sbjct: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKAT 300
           LTRIAQVVVAAARKW VDETR+ WRVCYEEDN AKN+ EG+HNLMTLARTNQFRILDKAT
Sbjct: 241 LTRIAQVVVAAARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFRILDKAT 300

Query: 301 LIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 360
           LIDKEDEARKKRDPWRLSTV EVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM
Sbjct: 301 LIDKEDEARKKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 360

Query: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 420
           LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI
Sbjct: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 420

Query: 421 SIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQEL 480
           SI TMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAI+GLQEL
Sbjct: 421 SILTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQEL 480

Query: 481 FYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWVL 540
           FYDQMP+FMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWL+DNLNRSNLHYFYWVL
Sbjct: 481 FYDQMPQFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLDDNLNRSNLHYFYWVL 540

Query: 541 AALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593
           AALSALNLCGYVWIANGFVYKR GGN RSI+GDD DVKNSNNINGCYGDDMI
Sbjct: 541 AALSALNLCGYVWIANGFVYKRVGGN-RSINGDDGDVKNSNNINGCYGDDMI 591

BLAST of CSPI05G27590 vs. ExPASy TrEMBL
Match: A0A5A7SVT8 (Protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007820 PE=3 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 567/593 (95.62%), Postives = 580/593 (97.81%), Query Frame = 0

Query: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60
           MEKQKSPNNVPIINLPNKFPDHPTASN+PTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN
Sbjct: 1   MEKQKSPNNVPIINLPNKFPDHPTASNTPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60

Query: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120
           LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASL+FF GM+
Sbjct: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMV 120

Query: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180
           VLTVSATVVGD+ RKAVFFLGLYILSVGQGGHRPCVQTFAADQF+E +PEERKKKSSFFN
Sbjct: 121 VLTVSATVVGDDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFN 180

Query: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240
           WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP
Sbjct: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQF-RILDKA 300
           LTRIAQVVVAAARKW VDETR+ WRVCYEEDN AKN+ EG+HNLMTLARTNQF RILDKA
Sbjct: 241 LTRIAQVVVAAARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFSRILDKA 300

Query: 301 TLIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGST 360
           TLIDKEDEARKKRDPWRLSTV EVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGST
Sbjct: 301 TLIDKEDEARKKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGST 360

Query: 361 MLRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLF 420
           MLRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLF
Sbjct: 361 MLRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLF 420

Query: 421 ISIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQE 480
           ISI TMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAI+GLQE
Sbjct: 421 ISILTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQE 480

Query: 481 LFYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWV 540
           LFYDQMP+FMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWL+DNLNRSNLHYFYWV
Sbjct: 481 LFYDQMPQFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLDDNLNRSNLHYFYWV 540

Query: 541 LAALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593
           LAALSALNLCGYVWIANGFVYKR GGN RSI+GDD DVKNSNNINGCYGDDMI
Sbjct: 541 LAALSALNLCGYVWIANGFVYKRVGGN-RSINGDDGDVKNSNNINGCYGDDMI 592

BLAST of CSPI05G27590 vs. ExPASy TrEMBL
Match: A0A6J1GWY8 (protein NRT1/ PTR FAMILY 5.4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458199 PE=3 SV=1)

HSP 1 Score: 921.4 bits (2380), Expect = 1.9e-264
Identity = 463/592 (78.21%), Postives = 513/592 (86.66%), Query Frame = 0

Query: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60
           ME++KSP   P+++LP K PDH   S+ P+  P GGWKAAIF+IFVEVAEQFA IGLSSN
Sbjct: 1   MEEEKSP--APLLHLPTKLPDHRNLSDRPSQPPAGGWKAAIFVIFVEVAEQFAFIGLSSN 60

Query: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120
           LIMYFTTVFHEP   AAK VNNW GVSAVFP+LGAFVADSLLGRFKTII +SLI+ +GM+
Sbjct: 61  LIMYFTTVFHEPTATAAKMVNNWSGVSAVFPILGAFVADSLLGRFKTIIFSSLIYCLGMV 120

Query: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180
           +LT+SATV+G   RK VFF  LYILSVG+GGHRPCVQTFAADQFDEE+PE+RK+KSSFFN
Sbjct: 121 LLTLSATVIGAPHRKPVFFFALYILSVGEGGHRPCVQTFAADQFDEETPEQRKRKSSFFN 180

Query: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240
           WWYVGLV GST AVF+VIYVQDNIGWGLSFGILAGVLAAA++LFL GVK YRR +PVGSP
Sbjct: 181 WWYVGLVVGSTLAVFLVIYVQDNIGWGLSFGILAGVLAAALLLFLCGVKVYRRHIPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKAT 300
           +TRIAQVVVAAARKWRVD TR  WRVCYEED+ AKN+ EG+H  MTL R +QF ILDKAT
Sbjct: 241 MTRIAQVVVAAARKWRVDNTRQEWRVCYEEDSHAKNEDEGQHKPMTLDRRSQFGILDKAT 300

Query: 301 LIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 360
           LID ED+ARKKRDPWRLSTV EVEEVK++ RLIPVW SCLMFAVVQAQIHTFFTKQGSTM
Sbjct: 301 LIDDEDKARKKRDPWRLSTVAEVEEVKMLGRLIPVWFSCLMFAVVQAQIHTFFTKQGSTM 360

Query: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 420
           LRS+GPHFQ+PPASLQGVVGLTILLTVLFYDRVFVP+AR FTGHHSGITVLQRIG+GLFI
Sbjct: 361 LRSIGPHFQIPPASLQGVVGLTILLTVLFYDRVFVPSARKFTGHHSGITVLQRIGIGLFI 420

Query: 421 SIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQEL 480
           SI  M  SALVEAKRV +AAEHGL DTPK TVPMTIWWLIPQYMLCGVSDAFA++GLQEL
Sbjct: 421 SILNMVASALVEAKRVAVAAEHGLIDTPKVTVPMTIWWLIPQYMLCGVSDAFAVVGLQEL 480

Query: 481 FYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWVL 540
           FYDQMPE MRS+G+AAYIS+IG+GNFLS+AIIS VQA S  +WL DNLNRS L YFYWVL
Sbjct: 481 FYDQMPESMRSIGSAAYISVIGIGNFLSTAIISAVQAASRHKWLVDNLNRSKLQYFYWVL 540

Query: 541 AALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593
           A LS LNLC Y+W+ANG+VYKR GG        +D  KNSN   G YGDD+I
Sbjct: 541 AGLSGLNLCCYIWVANGYVYKRVGG-----KDGEDHGKNSN--KGGYGDDII 583

BLAST of CSPI05G27590 vs. ExPASy TrEMBL
Match: A0A6J1GWF8 (protein NRT1/ PTR FAMILY 5.4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458199 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 7.3e-264
Identity = 464/593 (78.25%), Postives = 514/593 (86.68%), Query Frame = 0

Query: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60
           ME++KSP   P+++LP K PDH   S+ P+  P GGWKAAIF+IFVEVAEQFA IGLSSN
Sbjct: 1   MEEEKSP--APLLHLPTKLPDHRNLSDRPSQPPAGGWKAAIFVIFVEVAEQFAFIGLSSN 60

Query: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120
           LIMYFTTVFHEP   AAK VNNW GVSAVFP+LGAFVADSLLGRFKTII +SLI+ +GM+
Sbjct: 61  LIMYFTTVFHEPTATAAKMVNNWSGVSAVFPILGAFVADSLLGRFKTIIFSSLIYCLGMV 120

Query: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180
           +LT+SATV+G   RK VFF  LYILSVG+GGHRPCVQTFAADQFDEE+PE+RK+KSSFFN
Sbjct: 121 LLTLSATVIGAPHRKPVFFFALYILSVGEGGHRPCVQTFAADQFDEETPEQRKRKSSFFN 180

Query: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240
           WWYVGLV GST AVF+VIYVQDNIGWGLSFGILAGVLAAA++LFL GVK YRR +PVGSP
Sbjct: 181 WWYVGLVVGSTLAVFLVIYVQDNIGWGLSFGILAGVLAAALLLFLCGVKVYRRHIPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQF-RILDKA 300
           +TRIAQVVVAAARKWRVD TR  WRVCYEED+ AKN+ EG+H  MTL R +QF RILDKA
Sbjct: 241 MTRIAQVVVAAARKWRVDNTRQEWRVCYEEDSHAKNEDEGQHKPMTLDRRSQFGRILDKA 300

Query: 301 TLIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGST 360
           TLID ED+ARKKRDPWRLSTV EVEEVK++ RLIPVW SCLMFAVVQAQIHTFFTKQGST
Sbjct: 301 TLIDDEDKARKKRDPWRLSTVAEVEEVKMLGRLIPVWFSCLMFAVVQAQIHTFFTKQGST 360

Query: 361 MLRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLF 420
           MLRS+GPHFQ+PPASLQGVVGLTILLTVLFYDRVFVP+AR FTGHHSGITVLQRIG+GLF
Sbjct: 361 MLRSIGPHFQIPPASLQGVVGLTILLTVLFYDRVFVPSARKFTGHHSGITVLQRIGIGLF 420

Query: 421 ISIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQE 480
           ISI  M  SALVEAKRV +AAEHGL DTPK TVPMTIWWLIPQYMLCGVSDAFA++GLQE
Sbjct: 421 ISILNMVASALVEAKRVAVAAEHGLIDTPKVTVPMTIWWLIPQYMLCGVSDAFAVVGLQE 480

Query: 481 LFYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWV 540
           LFYDQMPE MRS+G+AAYIS+IG+GNFLS+AIIS VQA S  +WL DNLNRS L YFYWV
Sbjct: 481 LFYDQMPESMRSIGSAAYISVIGIGNFLSTAIISAVQAASRHKWLVDNLNRSKLQYFYWV 540

Query: 541 LAALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593
           LA LS LNLC Y+W+ANG+VYKR GG        +D  KNSN   G YGDD+I
Sbjct: 541 LAGLSGLNLCCYIWVANGYVYKRVGG-----KDGEDHGKNSN--KGGYGDDII 584

BLAST of CSPI05G27590 vs. NCBI nr
Match: XP_011655920.1 (protein NRT1/ PTR FAMILY 5.4 isoform X2 [Cucumis sativus] >KAE8648867.1 hypothetical protein Csa_008874 [Cucumis sativus])

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 591/592 (99.83%), Postives = 592/592 (100.00%), Query Frame = 0

Query: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60
           MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN
Sbjct: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60

Query: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120
           LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM
Sbjct: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120

Query: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180
           VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN
Sbjct: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180

Query: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240
           WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP
Sbjct: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKAT 300
           LTRIAQVVVAAARKWRVDETRNGWR+CYEEDNRAKNDAEGEHNLMTLARTNQFRILDKAT
Sbjct: 241 LTRIAQVVVAAARKWRVDETRNGWRICYEEDNRAKNDAEGEHNLMTLARTNQFRILDKAT 300

Query: 301 LIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 360
           LIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM
Sbjct: 301 LIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 360

Query: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 420
           LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI
Sbjct: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 420

Query: 421 SIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQEL 480
           SIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQEL
Sbjct: 421 SIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQEL 480

Query: 481 FYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWVL 540
           FYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWVL
Sbjct: 481 FYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWVL 540

Query: 541 AALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593
           AALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI
Sbjct: 541 AALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 592

BLAST of CSPI05G27590 vs. NCBI nr
Match: XP_031740965.1 (protein NRT1/ PTR FAMILY 5.4 isoform X1 [Cucumis sativus])

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 591/593 (99.66%), Postives = 592/593 (99.83%), Query Frame = 0

Query: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60
           MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN
Sbjct: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60

Query: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120
           LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM
Sbjct: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120

Query: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180
           VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN
Sbjct: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180

Query: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240
           WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP
Sbjct: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQF-RILDKA 300
           LTRIAQVVVAAARKWRVDETRNGWR+CYEEDNRAKNDAEGEHNLMTLARTNQF RILDKA
Sbjct: 241 LTRIAQVVVAAARKWRVDETRNGWRICYEEDNRAKNDAEGEHNLMTLARTNQFSRILDKA 300

Query: 301 TLIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGST 360
           TLIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGST
Sbjct: 301 TLIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGST 360

Query: 361 MLRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLF 420
           MLRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLF
Sbjct: 361 MLRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLF 420

Query: 421 ISIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQE 480
           ISIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQE
Sbjct: 421 ISIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQE 480

Query: 481 LFYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWV 540
           LFYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWV
Sbjct: 481 LFYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWV 540

Query: 541 LAALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593
           LAALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI
Sbjct: 541 LAALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593

BLAST of CSPI05G27590 vs. NCBI nr
Match: XP_008446958.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.4 [Cucumis melo] >TYK09312.1 protein NRT1/ PTR FAMILY 5.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 567/592 (95.78%), Postives = 580/592 (97.97%), Query Frame = 0

Query: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60
           MEKQKSPNNVPIINLPNKFPDHPTASN+PTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN
Sbjct: 1   MEKQKSPNNVPIINLPNKFPDHPTASNTPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60

Query: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120
           LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASL+FF GM+
Sbjct: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMV 120

Query: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180
           VLTVSATVVGD+ RKAVFFLGLYILSVGQGGHRPCVQTFAADQF+E +PEERKKKSSFFN
Sbjct: 121 VLTVSATVVGDDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFN 180

Query: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240
           WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP
Sbjct: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKAT 300
           LTRIAQVVVAAARKW VDETR+ WRVCYEEDN AKN+ EG+HNLMTLARTNQFRILDKAT
Sbjct: 241 LTRIAQVVVAAARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFRILDKAT 300

Query: 301 LIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 360
           LIDKEDEARKKRDPWRLSTV EVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM
Sbjct: 301 LIDKEDEARKKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 360

Query: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 420
           LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI
Sbjct: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 420

Query: 421 SIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQEL 480
           SI TMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAI+GLQEL
Sbjct: 421 SILTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQEL 480

Query: 481 FYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWVL 540
           FYDQMP+FMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWL+DNLNRSNLHYFYWVL
Sbjct: 481 FYDQMPQFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLDDNLNRSNLHYFYWVL 540

Query: 541 AALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593
           AALSALNLCGYVWIANGFVYKR GGN RSI+GDD DVKNSNNINGCYGDDMI
Sbjct: 541 AALSALNLCGYVWIANGFVYKRVGGN-RSINGDDGDVKNSNNINGCYGDDMI 591

BLAST of CSPI05G27590 vs. NCBI nr
Match: KAA0034758.1 (protein NRT1/ PTR FAMILY 5.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 567/593 (95.62%), Postives = 580/593 (97.81%), Query Frame = 0

Query: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60
           MEKQKSPNNVPIINLPNKFPDHPTASN+PTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN
Sbjct: 1   MEKQKSPNNVPIINLPNKFPDHPTASNTPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60

Query: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120
           LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASL+FF GM+
Sbjct: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMV 120

Query: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180
           VLTVSATVVGD+ RKAVFFLGLYILSVGQGGHRPCVQTFAADQF+E +PEERKKKSSFFN
Sbjct: 121 VLTVSATVVGDDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFN 180

Query: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240
           WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP
Sbjct: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQF-RILDKA 300
           LTRIAQVVVAAARKW VDETR+ WRVCYEEDN AKN+ EG+HNLMTLARTNQF RILDKA
Sbjct: 241 LTRIAQVVVAAARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFSRILDKA 300

Query: 301 TLIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGST 360
           TLIDKEDEARKKRDPWRLSTV EVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGST
Sbjct: 301 TLIDKEDEARKKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGST 360

Query: 361 MLRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLF 420
           MLRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLF
Sbjct: 361 MLRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLF 420

Query: 421 ISIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQE 480
           ISI TMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAI+GLQE
Sbjct: 421 ISILTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQE 480

Query: 481 LFYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWV 540
           LFYDQMP+FMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWL+DNLNRSNLHYFYWV
Sbjct: 481 LFYDQMPQFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLDDNLNRSNLHYFYWV 540

Query: 541 LAALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593
           LAALSALNLCGYVWIANGFVYKR GGN RSI+GDD DVKNSNNINGCYGDDMI
Sbjct: 541 LAALSALNLCGYVWIANGFVYKRVGGN-RSINGDDGDVKNSNNINGCYGDDMI 592

BLAST of CSPI05G27590 vs. NCBI nr
Match: XP_038892997.1 (protein NRT1/ PTR FAMILY 5.4 [Benincasa hispida])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.7e-299
Identity = 527/592 (89.02%), Postives = 553/592 (93.41%), Query Frame = 0

Query: 1   MEKQKSPNNVPIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSN 60
           MEK KS NN P+INL NKF D PTASN PT+ PGGGWKAAIFIIFVEVAEQFA IGLSSN
Sbjct: 1   MEKHKSLNN-PVINLSNKFLDRPTASNRPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSN 60

Query: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMM 120
           LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLI+ +GM+
Sbjct: 61  LIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIYCLGMV 120

Query: 121 VLTVSATVVGDNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180
           +LT+SATV+G + RK VFFL LYILSVG+GGHRPCVQTFAADQFDEESPEERKKKSSFFN
Sbjct: 121 LLTISATVIGADHRKPVFFLALYILSVGEGGHRPCVQTFAADQFDEESPEERKKKSSFFN 180

Query: 181 WWYVGLVGGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240
           WWYVGLV GSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP
Sbjct: 181 WWYVGLVVGSTFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSP 240

Query: 241 LTRIAQVVVAAARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKAT 300
           LTRIAQVVVAA RKWRVDETR+ WRVCY+E+N AKN+ E ++ LMTL RTNQFRILDKA 
Sbjct: 241 LTRIAQVVVAAVRKWRVDETRHEWRVCYDEENHAKNEGESQYKLMTLVRTNQFRILDKAA 300

Query: 301 LIDKEDEARKKRDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTM 360
           LID+EDEARKKR+PWRLSTV EVEEVKLVVRLIPVWVSCLMFAVVQAQ+HTFFTKQGSTM
Sbjct: 301 LIDEEDEARKKRNPWRLSTVREVEEVKLVVRLIPVWVSCLMFAVVQAQMHTFFTKQGSTM 360

Query: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFI 420
           LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFT HHSGITVLQRIGMGLFI
Sbjct: 361 LRSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTAHHSGITVLQRIGMGLFI 420

Query: 421 SIFTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQEL 480
           SI TMGVSALVEAKRVTIAA+HGL+DTPK T+PMTIWWLIPQYMLCGVSDAFAIIGLQEL
Sbjct: 421 SILTMGVSALVEAKRVTIAAQHGLTDTPKVTIPMTIWWLIPQYMLCGVSDAFAIIGLQEL 480

Query: 481 FYDQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWVL 540
           FYDQMP+ MRSLGAAAYISIIGVGNFLSSA+IS+VQAGSGG WLEDNLNRSNLHYFYWVL
Sbjct: 481 FYDQMPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSGGSWLEDNLNRSNLHYFYWVL 540

Query: 541 AALSALNLCGYVWIANGFVYKRAGGNRRSISGDDDDVKNSNNINGCYGDDMI 593
           AALSALNLCGYVWIANGFVYKR G        + DDVKNSNNINGCYGDDMI
Sbjct: 541 AALSALNLCGYVWIANGFVYKRVGD-----KNNGDDVKNSNNINGCYGDDMI 586

BLAST of CSPI05G27590 vs. TAIR 10
Match: AT3G54450.1 (Major facilitator superfamily protein )

HSP 1 Score: 625.2 bits (1611), Expect = 5.5e-179
Identity = 297/551 (53.90%), Postives = 413/551 (74.95%), Query Frame = 0

Query: 32  RPGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFHEPLGVAAKQVNNWVGVSAVFP 91
           R  GGW AA+FII VE+AE+FA  GL+SNLI + T    +    AAK +N W+GVS +FP
Sbjct: 11  RTKGGWNAALFIIVVEIAERFAFYGLASNLITFLTNELGQSTATAAKNINTWIGVSCMFP 70

Query: 92  LLGAFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVGDNQRKAVFFLGLYILSVGQGG 151
           +LGAF+ADS+LGRFKT+++ S I+ +G+++L +S TVV    R+ VFF+ LY+++VG+GG
Sbjct: 71  ILGAFLADSILGRFKTVLLTSFIYLLGIVMLPLSVTVVARRMREKVFFMALYVMAVGEGG 130

Query: 152 HRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGSTFAVFVVIYVQDNIGWGLSFG 211
           H+PCV TFAADQF E + EE+  K+SFFN+WY+ +V  S+ AV  +I++Q+ + W L F 
Sbjct: 131 HKPCVMTFAADQFGEANAEEKAAKTSFFNYWYMAIVLASSIAVLALIFIQERVSWSLGFS 190

Query: 212 ILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVAAARKWRVDETRNGWRVCYEED 271
           I+AG +  AI++FL G+ KYR+QVPVGSP TR+AQV+VAA +KWR+  TR+ + +CYEE+
Sbjct: 191 IIAGSVVIAIVIFLIGIPKYRKQVPVGSPFTRVAQVMVAALKKWRLSSTRHHYGLCYEEE 250

Query: 272 NRAKNDAEGEHNLMTLARTNQFRILDKATLIDKEDEARKKRDPWRLSTVEEVEEVKLVVR 331
           +  K ++   + +  LARTNQFR LDKAT+ID+ D   K R+PWRL TV +VEEVKL++R
Sbjct: 251 DEHKLESTNSNQVYLLARTNQFRFLDKATIIDEIDH-NKNRNPWRLCTVNQVEEVKLILR 310

Query: 332 LIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQLPPASLQGVVGLTILLTVLFYD 391
           LIP+W+S +MF     Q++TFF KQGS M R++G HF +PPA+ Q +VG+TIL+ +  YD
Sbjct: 311 LIPIWISLIMFCATLTQLNTFFLKQGSMMDRTIGNHFTIPPAAFQSIVGVTILILIPLYD 370

Query: 392 RVFVPAARNFTGHHSGITVLQRIGMGLFISIFTMGVSALVEAKRVTIAAEHGLSDTPKAT 451
           RVFVP  R  T HHSGIT LQRIG+GLF++ F M +  LVEAKR+ +A +HGL D+PK  
Sbjct: 371 RVFVPMVRKITNHHSGITSLQRIGVGLFVATFNMVICGLVEAKRLKVARDHGLIDSPKEV 430

Query: 452 VPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQMPEFMRSLGAAAYISIIGVGNFLSSAI 511
           VPM+  WL+PQY+L G+ D F I+G+QELFYDQMPE MRS+GAA +IS++GVG+F+S+ I
Sbjct: 431 VPMSSLWLLPQYILVGIGDVFTIVGMQELFYDQMPETMRSIGAAIFISVVGVGSFVSTGI 490

Query: 512 ISVVQAGS---GGRWLEDNLNRSNLHYFYWVLAALSALNLCGYVWIANGFVYKRAGGNRR 571
           IS VQ  S   G  WL +NLNR++L Y+YW++A+L+A++LC Y++IAN F+YK+      
Sbjct: 491 ISTVQTISKSHGEEWLVNNLNRAHLDYYYWIIASLNAVSLCFYLFIANHFLYKK------ 550

Query: 572 SISGDDDDVKN 580
            +   DDDV++
Sbjct: 551 -LQDKDDDVES 553

BLAST of CSPI05G27590 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 478.0 bits (1229), Expect = 1.1e-134
Identity = 255/555 (45.95%), Postives = 358/555 (64.50%), Query Frame = 0

Query: 27  NSPTVR-PGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFHEPLGVAAKQVNNWVG 86
           N P V+   GGW++A FII VEVAE+FA  G+SSNLI Y T    +    AA  VN W G
Sbjct: 23  NKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQSTAAAAANVNAWSG 82

Query: 87  VSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVGDNQ------------ 146
            +++ PLLGAFVADS LGRF+TI+ AS ++ +G+ VLT+SA +  D +            
Sbjct: 83  TASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSDCKVSNLLSSCSPRF 142

Query: 147 RKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGSTFA 206
           +   FF  LY++++ QGGH+PCVQ F ADQFDE+ PEE K KSSFFNWWY G+  G+   
Sbjct: 143 QVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSFFNWWYFGMCFGTLTT 202

Query: 207 VFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQV--PVGSPLTRIAQVVVAA 266
           ++V+ Y+QDN+ W L FGI    +  A+++ L G   YR  +     SP  RI  V VAA
Sbjct: 203 LWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQSPFVRIGNVYVAA 262

Query: 267 ARKWRVDETRNGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKATLIDKEDEARKK 326
            + W V             D  A   AE    L++ + + QF  L+KA L+ K       
Sbjct: 263 VKNWSVSAL----------DVAA---AEERLGLVSCSSSQQFSFLNKA-LVAKNGSC--- 322

Query: 327 RDPWRLSTVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQLP 386
                  +++E+EE K V+RL P+W++CL++AVV AQ  TFFTKQG+TM RS+ P +++ 
Sbjct: 323 -------SIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKIS 382

Query: 387 PASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFISIFTMGVSALV 446
           PA+LQ  + L+I++ +  YDRV +P AR+FT    GIT+LQRIG G+F+S   M V+ALV
Sbjct: 383 PATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAALV 442

Query: 447 EAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQMPEFMRS 506
           E KR+  AA++GL D+P ATVPM++WWL+PQY+L G++D FA++GLQE FYDQ+P  +RS
Sbjct: 443 EMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELRS 502

Query: 507 LGAAAYISIIGVGNFLSSAIISVVQAGSG----GRWLEDNLNRSNLHYFYWVLAALSALN 563
           +G A Y+SI G+GNFLSS +IS+++  +       W  +NLN+++L YFYW+LA LS + 
Sbjct: 503 VGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFIG 553

BLAST of CSPI05G27590 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 452.6 bits (1163), Expect = 4.9e-127
Identity = 244/549 (44.44%), Postives = 349/549 (63.57%), Query Frame = 0

Query: 35  GGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLG 94
           G W+AA FII VEVAE+FA+ G+ SNLI Y T    +   VAA  VN W G+S + PLLG
Sbjct: 31  GRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQSTAVAAANVNAWSGISTILPLLG 90

Query: 95  AFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVGDNQR---------KAVFFLGLYIL 154
           AFVAD+ LGR+ TIIIAS I+ +G+  LT+SA ++ +N            A+FF  LY++
Sbjct: 91  AFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNNTEVTSSPSSFLNALFFFSLYLV 150

Query: 155 SVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGSTFAVFVVIYVQDNIG 214
           ++GQ GH+PCVQ F ADQFDE++P+E   +SSFFNWWY+ +  G   A+ VV+Y+Q+N+ 
Sbjct: 151 AIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVS 210

Query: 215 WGLSFGILAGVLAAAIILFLAGVKKYR----RQVPVGSPLTRIAQVVVAAARKWRVDETR 274
           W L FGI    +  +++LF+ G K YR    RQ    +P TRI +V   A +  R++ + 
Sbjct: 211 WALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSS- 270

Query: 275 NGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKATLI----DKEDEARKKRDPWRL 334
           +  +V   E NR++   E            +   L+KA L+    D+ + A K RD    
Sbjct: 271 DLCKVELIEANRSQESPE------------ELSFLNKALLVPNDSDEGEVACKSRD---- 330

Query: 335 STVEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQLPPASLQG 394
                VE+   +VRLIPVW++ L +A+  AQ  TFFTKQG TM R++ P  ++PPASLQ 
Sbjct: 331 -----VEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPASLQV 390

Query: 395 VVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFISIFTMGVSALVEAKRVT 454
           ++ ++I+L V  YDRV VP  R+ T    GIT L+RIG G+ ++  TM V+ALVE+KR+ 
Sbjct: 391 LISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESKRLE 450

Query: 455 IAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQMPEFMRSLGAAAY 514
            A E+GL D PK T+PM+IWWL PQYML G++D   ++G+QE FY Q+P  +RSLG A Y
Sbjct: 451 TAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGLAIY 510

Query: 515 ISIIGVGNFLSSAIISVVQAGSGG----RWLEDNLNRSNLHYFYWVLAALSALNLCGYVW 563
           +S +GVG+ LSS +I ++   +GG     W   NLNR++L YFYW+LA +SA+    +++
Sbjct: 511 LSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFTFLF 557

BLAST of CSPI05G27590 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 442.6 bits (1137), Expect = 5.1e-124
Identity = 234/545 (42.94%), Postives = 339/545 (62.20%), Query Frame = 0

Query: 35  GGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLG 94
           G W+AA+FII VEVAE+FA  G+ SNLI Y T    E   VAA  VN W G++ + P+LG
Sbjct: 31  GRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAVAAANVNAWSGIATLLPVLG 90

Query: 95  AFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVGDNQR---------KAVFFLGLYIL 154
           AFVAD+ LGR++TIII+SLI+ +G+  LT+SA ++ +              +FF  LY++
Sbjct: 91  AFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEVTSSTSSFLNVLFFFSLYLV 150

Query: 155 SVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGSTFAVFVVIYVQDNIG 214
           ++GQ GH+PCVQ F ADQFDE+  +E+  +SSFFNWWY+ L  G  FA+ VV+Y+Q+   
Sbjct: 151 AIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWYLSLSAGICFAILVVVYIQEEFS 210

Query: 215 WGLSFGILAGVLAAAIILFLAGVKKY----RRQVPVGSPLTRIAQVVVAAARKWRVDETR 274
           W   FGI    +  +++LF++G + Y    RR     +P TRI +V   A +  R+  + 
Sbjct: 211 WAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRIGRVFFVALKNQRLSSS- 270

Query: 275 NGWRVCYEEDNRAKNDAEGEHNLMTLARTNQFRILDKATLIDKEDEARKKRDPWRLSTVE 334
               +C  E             L       +    +KA L+  +    +       S   
Sbjct: 271 ---DLCKVE-------------LEANTSPEKQSFFNKALLVPNDSSQGE-----NASKSS 330

Query: 335 EVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQLPPASLQGVVGL 394
           +VE+   ++RLIPVW + L +A+  AQ  TFFTKQG TM R++ P  ++PPASLQ  +G+
Sbjct: 331 DVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASLQVFIGI 390

Query: 395 TILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFISIFTMGVSALVEAKRVTIAAE 454
           +I+L V  YDRVFVP AR  T    GIT L+RIG G+ +S  TM ++ALVE KR+  A E
Sbjct: 391 SIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRLETAKE 450

Query: 455 HGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQMPEFMRSLGAAAYISII 514
           HGL D P+AT+PM+IWWLIPQY+L G++D + ++G+QE FY Q+P  +RS+G A Y+S +
Sbjct: 451 HGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLALYLSAL 510

Query: 515 GVGNFLSSAIISVVQAGSGG----RWLEDNLNRSNLHYFYWVLAALSALNLCGYVWIANG 563
           GVG+ LSS +IS++   +GG     W   NLNR++L YFYW+LA +SA+    +++I+  
Sbjct: 511 GVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGFFTFLFISKS 553

BLAST of CSPI05G27590 vs. TAIR 10
Match: AT3G53960.1 (Major facilitator superfamily protein )

HSP 1 Score: 428.7 bits (1101), Expect = 7.6e-120
Identity = 231/570 (40.53%), Postives = 354/570 (62.11%), Query Frame = 0

Query: 35  GGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFHEPLGVAAKQVNNWVGVSAVFPLLG 94
           G W+AA+FII +E +E+ +  G+S+NL++Y TT+ H+ L +A K  N W GV+ + PLLG
Sbjct: 39  GAWRAALFIIGIEFSERLSYFGISTNLVVYLTTILHQDLKMAVKNTNYWSGVTTLMPLLG 98

Query: 95  AFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVG---------DNQRKA---VFFLGL 154
            FVAD+ LGR+ T+++A+ I+ +G+++LT+S  + G            RKA    FF+ +
Sbjct: 99  GFVADAYLGRYGTVLLATTIYLMGLILLTLSWFIPGLKACHEDMCVEPRKAHEIAFFIAI 158

Query: 155 YILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGSTFAVFVVIYVQD 214
           Y++S+G GGH+P +++F ADQF++  PEERK K S+FNWW  GL  G   AV V++Y++D
Sbjct: 159 YLISIGTGGHKPSLESFGADQFEDGHPEERKMKMSYFNWWNAGLCAGILTAVTVIVYIED 218

Query: 215 NIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVAAARKWRVDETRN 274
            IGWG++  IL  V+A +  +F  G   YR + P GSPLT + QV VAA  K  +     
Sbjct: 219 RIGWGVASIILTIVMATSFFIFRIGKPFYRYRAPSGSPLTPMLQVFVAAIAKRNLP---- 278

Query: 275 GWRVCYEEDNRAKNDAEGEHNL-MTLARTNQFRILDKATLIDKEDEARK--KRDPWRLST 334
               C  + +        E+     L+ +   + LDKA +I+  +E  K  K+ PWRL+T
Sbjct: 279 ----CPSDSSLLHELTNEEYTKGRLLSSSKNLKFLDKAAVIEDRNENTKAEKQSPWRLAT 338

Query: 335 VEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSV-GPHFQLPPASLQGV 394
           V +VEEVKL++ +IP+W   L F V   Q  T F KQ   M R + G  F +PPASL  +
Sbjct: 339 VTKVEEVKLLINMIPIWFFTLAFGVCATQSSTLFIKQAIIMDRHITGTSFIVPPASLFSL 398

Query: 395 VGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFISIFTMGVSALVEAKRVTI 454
           + L+I++TV  Y+++ VP  R  TG+  GI++LQRIG+G+  S+F M ++AL+E KR+  
Sbjct: 399 IALSIIITVTIYEKLLVPLLRRATGNERGISILQRIGVGMVFSLFAMIIAALIEKKRLDY 458

Query: 455 AAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQMPEFMRSLGAAAYI 514
           A EH ++     T+ ++  WL PQ+++ GV+DAF ++GLQE FYDQ+P+ MRSLG A Y+
Sbjct: 459 AKEHHMN----KTMTLSAIWLAPQFLVLGVADAFTLVGLQEYFYDQVPDSMRSLGIAFYL 518

Query: 515 SIIGVGNFLSSAIISV----VQAGSGGRWLEDNLNRSNLHYFYWVLAALSALNLCGYVWI 574
           S++G  +F+++ +I+V     +  SG  W   +LN S L  FYW+LAAL+A N+C +V +
Sbjct: 519 SVLGAASFVNNLLITVSDHLAEEISGKGWFGKDLNSSRLDRFYWMLAALTAANICCFVIV 578

Query: 575 ANGFVYKRAGGNRRSISGDDDDVKNSNNIN 585
           A  + YK    +   ++   DDV+ +   N
Sbjct: 579 AMRYTYKTVQPSLAVVADGGDDVETATGTN 596