CSPI05G27150 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G27150
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionmRNA-decapping enzyme subunit 2-like
LocationChr5: 25767931 .. 25771627 (+)
RNA-Seq ExpressionCSPI05G27150
SyntenyCSPI05G27150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAGCAGAGTGTAGACAGTAGATTCTTTTTGGCGATCTTCATCATACCATATAGCCATGGCCGGCCTCCATCTGCCCTCTAATGCTCATGTGAAGAACGGCCTTCCACCCCAAGAACTTCTCGATGATCTTTGCAGGTACGCGCTTTGTTTCTGTTTCTCTCTATTTGATTTACGTTTTGCTGTGAGCTATTTGTTTGTTAGATGTGGCGATCTTACGGTTTTTAGCTGAATTTGATACGAGCTGGTTTATTCTAATTATCTTTCTTTTTATTTATCCACCTGATTGTCGTATATTGTTTTTGCAAAACGAAACTATGTCAGTTAGAACTATTAATTAGGCATCTTGTTGAAGAAAATAACATGATGGGTAAAACATTGGACCATTTCCTGAAGCGAATGATCATAATTCTTCCAAAAGTGCTTGTCCTTGAATGGGTTTTTTCCCTCCCTCTGACCCTACAATAAGCAAAAAAATATACTCTTAAACGGATTTTGGAAGCCTACCTTCATTCAAATGAGTCAATTGACAGTTTAGATCTTATTTGATAATGATTTTGTTATTAGTTTTATGTTTTTGAAATTTATGTTTGTTTCTTGCCACTTTCTTTACTGGCCTTCACCTTTCTTGAAGAAACATTCCATAAATACAAACGTGTTTTAAAAATGTAATTTTTATAGTTTCCGAAACCACCCATGTAGAGGTGTCATTTTGATGCCTTCTTTTTCCCTCATATGCAGCCGATTTGTCTTAAACGTGCCAAAGGAAGATCTACAATCATTTGAGAGGATTTTGTTTCTAGTTGAATATGCCCATTGGTTCTACGAAGACAACTCGGTTGAAAACAATCCTTCACTGAAGTCCTTCAATTTGAAGGAGTTCACTTCATTGTGTATCCTAAGCATCATGCAATCCATTACCTTAACAATGTGCTTGGATGTTGTTTTCAATGTTCTGTTTCTATGGTAAATAAATGCAACTCTATTTATTAACTTTATCATTTATTTATTTAGCATATGATTAATTTGATTATACCACTGCTTTGAGCTAGTGTTATCTCTATCTCAATTTGTGGTAAACTGTTTATGCACCCACACATACATAACATATTCTTGAGTCTGTCAAACCCGTACTTTCACTTATAAGTCTGAATTTATGGCATCTCTGTATGGTTGCATGATGCTTTCTTATTTGCTTATGCTACTTTTCATGGATATCTTTTTCTTCCTTAATCTTCTACAGTATTTAATAGTTGTGATGTTTTGAAACCATATGTGCCCCACATAGACGACATATTTAAGGACTTCACGTCTTATAAGCTTCGAGTTCCTGTCACTGGGGGAATTATTTTGGATGAAACCTTTGAGAGGGTACGTTTTGGTTTGTTATTATTTAAAAAATGGTAGATGTGAGGAGTACTTATACTCTATTTGTGCCTCCTTTTCTCTCTCAACCAAACAGTGCTTACTAGTGAAGGGATGGAAGGGGTCAAGCTGGAGTTTTCCTCGTGGAAAAAAGAGCAAGGATGAAGAAGATCACGCCTGTGCTATTAGAGAAGTAATTTTTCTTTCTTATTTTGGACTTCGATCACAGTAATTAGTATTTCGTGTTTGAAAATGCCAACTTTTATTGAGAAAAAATGAATTGAGTATGAAAATATTATAGAAAATACATAAACCATGAGAGAGAGGAAAAAAACTCTAAAATCTTTTGGTACAAAGATTCGAAGGGAAGCATTGAACCTCTGTAAGGCCCAAAATTCCTCCCAAGAACTCACCACAACTCTTCCCTTATTTCTCTACTGTGAAATATACCATATTATCACAGAGAAAGTGCCCTGCCAAACAGTCACAATCTATCATGAAAAGGAGGGTGAAATAGTAGTTCCTGGACTGTAAATGGTTATCTTATCGTACCTCCTCCTATGGCACAAAGCTTCAGGTTTATGGGCTCAAACTGGTTGTTTTTCATCTGATATTATTTGTATTAAGCAAAAAGAACACCAAGGAGGTTTTGTCGCTTCCGGGCTTCAACTGAATTACTTATGTCTGTATCAAATCAAGTTGGTACTTTGGCACTTATCTCACCATGGAATATATTTTGGATTGAGAATGTAGGTTTTGGAGGAAACTGGTTTTGATGTTACACCGTTCCTTATCAAAGAAGATTTTATTGAAGTGATGTTTGGACAACAGAGAGTGCGGCTCTACATTATAGCTGGGGTGAAAAATGATACTGCCTTTGCACCTCTTACTAAGAAAGAGATCAGTGTATGTCTTCCTTCTTCGTTTTTCTATCAGTTTAAGGCTGATTCTATTCGGTTAAAAACTATATTTGCCCTCTTTCCTACCTTTTTCATTAATATTTGTGATGTTTATATATATACATATATACTTCATTGACAACGTATCACTCTAATGCTGATTTTTTGAGTCTTGTGAAGCACAATGTATAATGGATATGTAATTTGGAAGCAGGAAATTGCGTGGCATCGACTTGACGACCTTCTACCTGTGAGTGATGATGTGATATCTCATGGCATCACCGGCCTTAAGCTTTACATGGTGGCTCCTTTCTTGGAGTGAGCAGTTTTCTTGATATTTTCTGCACGCAGTTAGAAATTGATTTATGTTTAATGAATTCGACAATTTTAAATTGCAGGTCATTAAGATCGTGGATTTTGAAGCATCAGCCACCCGTGGCTCCTAACTTTGACATGCCTGTGAAAGGTAATCTGTTTGCGACTTGCATATTCTTTAAAAGGGAATTGGTAACGTAAGGAAAGTTTCCTAGGCATATGAACTATCAAGAGTGGTTTTATCAAGCAAATGTAACCTTCAAAATCAAAATGGAAAACGAAAACAGGTAAATAGCAACCTCATGAACAATATCTTAGATGAAAAATTGAAAAGAAGAAGTCGTTTTTATTTGTTTGGATTGAGTTTCCTATTATATTAGGTCTCGTAGGAAAAGAATGGAATCATACATCTTCCAAGAGATCTACTAGAACCATGCCTGTTAGCGAGGTTCCAAATTCTCGATTCTATTGTTGCCATAGCCACGATATTAACAAGAAACCAACTCAGTTGGTGTGACTACATAATCCTTAAGTTACCGTCCATCAAAGTTATACGCATTATTTCTTTGTTGTATTAATCCGTGGTCTCAAACACATTTTCTATACTTTGCAAACCACTAAGCCTCTCAACTTTCTTGCATTCTTCGCCAATGAGCTCTATTAACGCAGCTATAAATTGTTGCTTTCCACTCAGGATTCACCATGTGGAAGGTGAAGAATACCTCGATGAGCAATAGCTCAATCATTTTCGACAACCAGCCGATTAAATTGGATTCTGATCCTGGAAGAAGCCTCAGAAACTTCAAATTCGACACAGCCTCTATCTTGCACGCTATGGAAAGTGCTTTTGCTGCATGAATTTCAACAGGGAATGCATATTGTCCAACTGACTGGTAGGCTCGGTTTCATTTTGTTTTCTTTTTTTATAATTGTTGTGTAGTACATTTAGGTAATATTGTGCATATATGCCTAATTTTGTTCTTGATTTTAAATGTAGATTATGTACAGACCCGTTTAGCGATTTTACTTATCTGGTTTTTGGTTGTATAGAATAACAACATAGGAGATATGATTCTAACTCAAAGCACTTTGAATAATTCTAACTCACCACTCATAGCTG

mRNA sequence

TGAGCAGAGTGTAGACAGTAGATTCTTTTTGGCGATCTTCATCATACCATATAGCCATGGCCGGCCTCCATCTGCCCTCTAATGCTCATGTGAAGAACGGCCTTCCACCCCAAGAACTTCTCGATGATCTTTGCAGCCGATTTGTCTTAAACGTGCCAAAGGAAGATCTACAATCATTTGAGAGGATTTTGTTTCTAGTTGAATATGCCCATTGGTTCTACGAAGACAACTCGGTTGAAAACAATCCTTCACTGAAGTCCTTCAATTTGAAGGAGTTCACTTCATTGTTATTTAATAGTTGTGATGTTTTGAAACCATATGTGCCCCACATAGACGACATATTTAAGGACTTCACGTCTTATAAGCTTCGAGTTCCTGTCACTGGGGGAATTATTTTGGATGAAACCTTTGAGAGGTGCTTACTAGTGAAGGGATGGAAGGGGTCAAGCTGGAGTTTTCCTCGTGGAAAAAAGAGCAAGGATGAAGAAGATCACGCCTGTGCTATTAGAGAAGTTTTGGAGGAAACTGGTTTTGATGTTACACCGTTCCTTATCAAAGAAGATTTTATTGAAGTGATGTTTGGACAACAGAGAGTGCGGCTCTACATTATAGCTGGGGTGAAAAATGATACTGCCTTTGCACCTCTTACTAAGAAAGAGATCAGTGAAATTGCGTGGCATCGACTTGACGACCTTCTACCTGTGAGTGATGATGTGATATCTCATGGCATCACCGGCCTTAAGCTTTACATGGTGGCTCCTTTCTTGGAGTCATTAAGATCGTGGATTTTGAAGCATCAGCCACCCGTGGCTCCTAACTTTGACATGCCTGTGAAAGGATTCACCATGTGGAAGGTGAAGAATACCTCGATGAGCAATAGCTCAATCATTTTCGACAACCAGCCGATTAAATTGGATTCTGATCCTGGAAGAAGCCTCAGAAACTTCAAATTCGACACAGCCTCTATCTTGCACGCTATGGAAAGTGCTTTTGCTGCATGAATTTCAACAGGGAATGCATATTGTCCAACTGACTGGTAGGCTCGGTTTCATTTTGTTTTCTTTTTTTATAATTGTTGTGTAGTACATTTAGGTAATATTGTGCATATATGCCTAATTTTGTTCTTGATTTTAAATGTAGATTATGTACAGACCCGTTTAGCGATTTTACTTATCTGGTTTTTGGTTGTATAGAATAACAACATAGGAGATATGATTCTAACTCAAAGCACTTTGAATAATTCTAACTCACCACTCATAGCTG

Coding sequence (CDS)

ATGGCCGGCCTCCATCTGCCCTCTAATGCTCATGTGAAGAACGGCCTTCCACCCCAAGAACTTCTCGATGATCTTTGCAGCCGATTTGTCTTAAACGTGCCAAAGGAAGATCTACAATCATTTGAGAGGATTTTGTTTCTAGTTGAATATGCCCATTGGTTCTACGAAGACAACTCGGTTGAAAACAATCCTTCACTGAAGTCCTTCAATTTGAAGGAGTTCACTTCATTGTTATTTAATAGTTGTGATGTTTTGAAACCATATGTGCCCCACATAGACGACATATTTAAGGACTTCACGTCTTATAAGCTTCGAGTTCCTGTCACTGGGGGAATTATTTTGGATGAAACCTTTGAGAGGTGCTTACTAGTGAAGGGATGGAAGGGGTCAAGCTGGAGTTTTCCTCGTGGAAAAAAGAGCAAGGATGAAGAAGATCACGCCTGTGCTATTAGAGAAGTTTTGGAGGAAACTGGTTTTGATGTTACACCGTTCCTTATCAAAGAAGATTTTATTGAAGTGATGTTTGGACAACAGAGAGTGCGGCTCTACATTATAGCTGGGGTGAAAAATGATACTGCCTTTGCACCTCTTACTAAGAAAGAGATCAGTGAAATTGCGTGGCATCGACTTGACGACCTTCTACCTGTGAGTGATGATGTGATATCTCATGGCATCACCGGCCTTAAGCTTTACATGGTGGCTCCTTTCTTGGAGTCATTAAGATCGTGGATTTTGAAGCATCAGCCACCCGTGGCTCCTAACTTTGACATGCCTGTGAAAGGATTCACCATGTGGAAGGTGAAGAATACCTCGATGAGCAATAGCTCAATCATTTTCGACAACCAGCCGATTAAATTGGATTCTGATCCTGGAAGAAGCCTCAGAAACTTCAAATTCGACACAGCCTCTATCTTGCACGCTATGGAAAGTGCTTTTGCTGCATGA

Protein sequence

MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRVRLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESLRSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFDTASILHAMESAFAA*
Homology
BLAST of CSPI05G27150 vs. ExPASy Swiss-Prot
Match: Q8GW31 (mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.1e-119
Identity = 205/284 (72.18%), Postives = 239/284 (84.15%), Query Frame = 0

Query: 1   MAGLHLPSNA--HVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDN 60
           M+GLH  S++  ++ N LP +ELLDDLCSRFVLNVP+ED QSFERILFLVEYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETF 120
           +VEN+P LKS +LKEFTSLLFNSCDVL+PYV HIDDIFKDFTSYK RVPVTG IILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQ 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+  L +E++IE +F QQ
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQ 180

Query: 181 RVRLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLE 240
           RVRLYI+AGV  DT FAPLTKKEISEI WHRLD L P S++VI+HG++GLKLYMVAPFL 
Sbjct: 181 RVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLS 240

Query: 241 SLRSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQ 283
           SL+SWILKH  PVA   + P+K   +W  + +   N +   ++Q
Sbjct: 241 SLKSWILKHPSPVARRPNKPLKALCVWNARTSVGGNGTATVESQ 284

BLAST of CSPI05G27150 vs. ExPASy Swiss-Prot
Match: O13828 (mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dcp2 PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.2e-53
Identity = 108/228 (47.37%), Postives = 140/228 (61.40%), Query Frame = 0

Query: 20  ELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSLLF 79
           ++LDDL +RF+LN+P E+  S ER+ F +E AHWFYED     N  L S  L+ F++ LF
Sbjct: 10  QVLDDLSARFILNLPAEEQSSVERLCFQIEQAHWFYEDFIRAQNDQLPSLGLRVFSAKLF 69

Query: 80  NSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFERCLLVKGWKGSS-WSFPRGK 139
             C +L  +    ++ F DF  YK R+PV G I+LD + ++C+LVKGWK SS W FP+GK
Sbjct: 70  AHCPLLWKWSKVHEEAFDDFLRYKTRIPVRGAIMLDMSMQQCVLVKGWKASSGWGFPKGK 129

Query: 140 KSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRVRLYIIAGVKNDTAFAPLT 199
             KDE D  CAIREV EETGFD +  +   +FI++    Q VRLYII G+  DT F   T
Sbjct: 130 IDKDESDVDCAIREVYEETGFDCSSRINPNEFIDMTIRGQNVRLYIIPGISLDTRFESRT 189

Query: 200 KKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESLRSWILK 247
           +KEIS+I WH L DL     +         K YMV PFL  L+ WI K
Sbjct: 190 RKEISKIEWHNLMDLPTFKKN--KPQTMKNKFYMVIPFLAPLKKWIKK 235

BLAST of CSPI05G27150 vs. ExPASy Swiss-Prot
Match: Q9CYC6 (m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2)

HSP 1 Score: 206.5 bits (524), Expect = 4.6e-52
Identity = 98/231 (42.42%), Postives = 138/231 (59.74%), Query Frame = 0

Query: 18  PQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSL 77
           P  +LDDLCSRF+L++P E+  +  R+ F +E AHWFY D  ++N P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKA 68

Query: 78  LFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFERCLLVKGWKG-SSWSFPR 137
           +F+ C  L P    ++ I  ++  YK+ VP  G IILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPQGEDVEKILDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 138 GKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRVRLYIIAGVKNDTAFAP 197
           GK +K+E  H CA REV EETGFD+  ++ K+D+IE+    Q  RLYII GV  DT F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGVPKDTKFNP 188

Query: 198 LTKKEISEIAWHRLDDLLPVSDDVISHGITGL---KLYMVAPFLESLRSWI 245
            T++EI  I W  ++ L    +D+      GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of CSPI05G27150 vs. ExPASy Swiss-Prot
Match: Q8IU60 (m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3)

HSP 1 Score: 205.7 bits (522), Expect = 7.8e-52
Identity = 96/231 (41.56%), Postives = 138/231 (59.74%), Query Frame = 0

Query: 18  PQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSL 77
           P  +LDDLCSRF+L++P E+  +  R+ F +E AHWFY D  ++N P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKA 68

Query: 78  LFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFERCLLVKGWKG-SSWSFPR 137
           +F+ C  L P    ++ +  ++  YK+ VP  G IILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 138 GKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRVRLYIIAGVKNDTAFAP 197
           GK +K+E  H CA REV EETGFD+  ++ K+D+IE+    Q  RLYII G+  DT F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNP 188

Query: 198 LTKKEISEIAWHRLDDLLPVSDDVISHGITGL---KLYMVAPFLESLRSWI 245
            T++EI  I W  ++ L    +D+      GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of CSPI05G27150 vs. ExPASy Swiss-Prot
Match: Q5REQ8 (m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 7.8e-52
Identity = 96/231 (41.56%), Postives = 138/231 (59.74%), Query Frame = 0

Query: 18  PQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSVENNPSLKSFNLKEFTSL 77
           P  +LDDLCSRF+L++P E+  +  R+ F +E AHWFY D  ++N P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLSQCGIRDFAKA 68

Query: 78  LFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFERCLLVKGWKG-SSWSFPR 137
           +F+ C  L P    ++ +  ++  YK+ VP  G IILDET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPEGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 138 GKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRVRLYIIAGVKNDTAFAP 197
           GK +K+E  H CA REV EETGFD+  ++ K+D+IE+    Q  RLYII G+  DT F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNP 188

Query: 198 LTKKEISEIAWHRLDDLLPVSDDVISHGITGL---KLYMVAPFLESLRSWI 245
            T++EI  I W  ++ L    +D+      GL   K +M  PF+  LR W+
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWL 239

BLAST of CSPI05G27150 vs. ExPASy TrEMBL
Match: A0A0A0KWT4 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G622860 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 9.8e-183
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240
           RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300
           RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300

Query: 301 TASILHAMESAFAA 315
           TASILHAMESAFAA
Sbjct: 301 TASILHAMESAFAA 314

BLAST of CSPI05G27150 vs. ExPASy TrEMBL
Match: A0A5D3CCQ6 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002700 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.1e-178
Identity = 308/314 (98.09%), Postives = 311/314 (99.04%), Query Frame = 0

Query: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MA LHLPSNA VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MASLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLF+SCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFSSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240
           RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300
           RSWILKHQPPVAPNFDMPVKG+TMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSL+NFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGYTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLKNFKFD 300

Query: 301 TASILHAMESAFAA 315
           TASIL AMESAFAA
Sbjct: 301 TASILQAMESAFAA 314

BLAST of CSPI05G27150 vs. ExPASy TrEMBL
Match: A0A1S3BG59 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103489466 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.1e-178
Identity = 308/314 (98.09%), Postives = 311/314 (99.04%), Query Frame = 0

Query: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MA LHLPSNA VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MASLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLF+SCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFSSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240
           RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300
           RSWILKHQPPVAPNFDMPVKG+TMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSL+NFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGYTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLKNFKFD 300

Query: 301 TASILHAMESAFAA 315
           TASIL AMESAFAA
Sbjct: 301 TASILQAMESAFAA 314

BLAST of CSPI05G27150 vs. ExPASy TrEMBL
Match: A0A6J1CC63 (mRNA-decapping enzyme subunit 2-like OS=Momordica charantia OX=3673 GN=LOC111010222 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.4e-165
Identity = 286/314 (91.08%), Postives = 296/314 (94.27%), Query Frame = 0

Query: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLH PS A VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHQPSIAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTG IILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+ FLIKEDFIE+MFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180

Query: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240
           RLYIIAGVKND++FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240

Query: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300
           RSWIL HQ PV PNFDMP+KG TMWKVKN+SMS+SS++ DNQ  K DSDPG+S RNFKFD
Sbjct: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300

Query: 301 TASILHAMESAFAA 315
           TASIL AME AFAA
Sbjct: 301 TASILQAMEGAFAA 314

BLAST of CSPI05G27150 vs. ExPASy TrEMBL
Match: A0A6J1IK27 (mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477819 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.1e-165
Identity = 286/314 (91.08%), Postives = 294/314 (93.63%), Query Frame = 0

Query: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAG+H P+NA VKNGLPPQELLDDLCSRFVLNVP+EDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGIHQPANASVKNGLPPQELLDDLCSRFVLNVPREDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLI++DF+EVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIEDDFLEVMFGQQRV 180

Query: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240
           RLYIIAGVK D  FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKTDIPFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLNSL 240

Query: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300
           RSWILKHQPPV PNFDM VKG TMWKVK     NSSI+FD Q  K DSDPG+SLRNFKFD
Sbjct: 241 RSWILKHQPPVNPNFDMHVKGVTMWKVK-----NSSIVFDGQQTKFDSDPGKSLRNFKFD 300

Query: 301 TASILHAMESAFAA 315
           TASIL AMESAFAA
Sbjct: 301 TASILQAMESAFAA 309

BLAST of CSPI05G27150 vs. NCBI nr
Match: XP_004142410.1 (mRNA-decapping enzyme subunit 2 [Cucumis sativus] >XP_031742317.1 mRNA-decapping enzyme subunit 2 [Cucumis sativus] >KGN52271.1 hypothetical protein Csa_007962 [Cucumis sativus])

HSP 1 Score: 649.0 bits (1673), Expect = 2.0e-182
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240
           RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300
           RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300

Query: 301 TASILHAMESAFAA 315
           TASILHAMESAFAA
Sbjct: 301 TASILHAMESAFAA 314

BLAST of CSPI05G27150 vs. NCBI nr
Match: XP_008446886.1 (PREDICTED: mRNA-decapping enzyme subunit 2-like [Cucumis melo] >KAA0034722.1 mRNA-decapping enzyme subunit 2-like [Cucumis melo var. makuwa] >TYK09275.1 mRNA-decapping enzyme subunit 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 635.6 bits (1638), Expect = 2.3e-178
Identity = 308/314 (98.09%), Postives = 311/314 (99.04%), Query Frame = 0

Query: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MA LHLPSNA VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MASLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLF+SCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFSSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240
           RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300
           RSWILKHQPPVAPNFDMPVKG+TMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSL+NFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGYTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLKNFKFD 300

Query: 301 TASILHAMESAFAA 315
           TASIL AMESAFAA
Sbjct: 301 TASILQAMESAFAA 314

BLAST of CSPI05G27150 vs. NCBI nr
Match: XP_038891421.1 (mRNA-decapping enzyme subunit 2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 628.6 bits (1620), Expect = 2.8e-176
Identity = 306/314 (97.45%), Postives = 308/314 (98.09%), Query Frame = 0

Query: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLHLPSNA VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREV EETGFDVT FLIKEDFIEVMFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVSEETGFDVTAFLIKEDFIEVMFGQQRV 180

Query: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240
           RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL
Sbjct: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240

Query: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300
           RSWILKHQPPVAPNFDMPVKG T+WKVKN+SMSNSSIIFD Q IKLDSDPGRSLRNFKFD
Sbjct: 241 RSWILKHQPPVAPNFDMPVKGVTIWKVKNSSMSNSSIIFDGQSIKLDSDPGRSLRNFKFD 300

Query: 301 TASILHAMESAFAA 315
           TASILHAMESAFAA
Sbjct: 301 TASILHAMESAFAA 314

BLAST of CSPI05G27150 vs. NCBI nr
Match: XP_038891420.1 (mRNA-decapping enzyme subunit 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 615.9 bits (1587), Expect = 1.9e-172
Identity = 303/316 (95.89%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLHLPSNA VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHLPSNAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNS--CDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETF 120
           ENNPSLKSFNLKEFTSL   S  CDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETF
Sbjct: 61  ENNPSLKSFNLKEFTSLCILSIICDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETF 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQ 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREV EETGFDVT FLIKEDFIEVMFGQQ
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVSEETGFDVTAFLIKEDFIEVMFGQQ 180

Query: 181 RVRLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLE 240
           RVRLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLE
Sbjct: 181 RVRLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLE 240

Query: 241 SLRSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFK 300
           SLRSWILKHQPPVAPNFDMPVKG T+WKVKN+SMSNSSIIFD Q IKLDSDPGRSLRNFK
Sbjct: 241 SLRSWILKHQPPVAPNFDMPVKGVTIWKVKNSSMSNSSIIFDGQSIKLDSDPGRSLRNFK 300

Query: 301 FDTASILHAMESAFAA 315
           FDTASILHAMESAFAA
Sbjct: 301 FDTASILHAMESAFAA 316

BLAST of CSPI05G27150 vs. NCBI nr
Match: XP_022139266.1 (mRNA-decapping enzyme subunit 2-like [Momordica charantia])

HSP 1 Score: 591.3 bits (1523), Expect = 5.0e-165
Identity = 286/314 (91.08%), Postives = 296/314 (94.27%), Query Frame = 0

Query: 1   MAGLHLPSNAHVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60
           MAGLH PS A VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV
Sbjct: 1   MAGLHQPSIAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDNSV 60

Query: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETFER 120
           ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTG IILDETFER
Sbjct: 61  ENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGAIILDETFER 120

Query: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQRV 180
           CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+ FLIKEDFIE+MFGQQRV
Sbjct: 121 CLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSAFLIKEDFIELMFGQQRV 180

Query: 181 RLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLESL 240
           RLYIIAGVKND++FAPLTKKEISEIAWHRLDDLLP SDDVISHGITGLKLYMVAPFL SL
Sbjct: 181 RLYIIAGVKNDSSFAPLTKKEISEIAWHRLDDLLPASDDVISHGITGLKLYMVAPFLTSL 240

Query: 241 RSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQPIKLDSDPGRSLRNFKFD 300
           RSWIL HQ PV PNFDMP+KG TMWKVKN+SMS+SS++ DNQ  K DSDPG+S RNFKFD
Sbjct: 241 RSWILTHQRPVVPNFDMPLKGVTMWKVKNSSMSSSSMVLDNQSSKSDSDPGKSFRNFKFD 300

Query: 301 TASILHAMESAFAA 315
           TASIL AME AFAA
Sbjct: 301 TASILQAMEGAFAA 314

BLAST of CSPI05G27150 vs. TAIR 10
Match: AT5G13570.1 (decapping 2 )

HSP 1 Score: 431.0 bits (1107), Expect = 8.1e-121
Identity = 205/284 (72.18%), Postives = 239/284 (84.15%), Query Frame = 0

Query: 1   MAGLHLPSNA--HVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDN 60
           M+GLH  S++  ++ N LP +ELLDDLCSRFVLNVP+ED QSFERILFLVEYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETF 120
           +VEN+P LKS +LKEFTSLLFNSCDVL+PYV HIDDIFKDFTSYK RVPVTG IILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVTPFLIKEDFIEVMFGQQ 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDV+  L +E++IE +F QQ
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQ 180

Query: 181 RVRLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGITGLKLYMVAPFLE 240
           RVRLYI+AGV  DT FAPLTKKEISEI WHRLD L P S++VI+HG++GLKLYMVAPFL 
Sbjct: 181 RVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLS 240

Query: 241 SLRSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQ 283
           SL+SWILKH  PVA   + P+K   +W  + +   N +   ++Q
Sbjct: 241 SLKSWILKHPSPVARRPNKPLKALCVWNARTSVGGNGTATVESQ 284

BLAST of CSPI05G27150 vs. TAIR 10
Match: AT5G13570.2 (decapping 2 )

HSP 1 Score: 421.8 bits (1083), Expect = 4.9e-118
Identity = 205/297 (69.02%), Postives = 239/297 (80.47%), Query Frame = 0

Query: 1   MAGLHLPSNA--HVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLVEYAHWFYEDN 60
           M+GLH  S++  ++ N LP +ELLDDLCSRFVLNVP+ED QSFERILFLVEYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVENNPSLKSFNLKEFTSLLFNSCDVLKPYVPHIDDIFKDFTSYKLRVPVTGGIILDETF 120
           +VEN+P LKS +LKEFTSLLFNSCDVL+PYV HIDDIFKDFTSYK RVPVTG IILDET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRE-------------VLEETGFDVTPFL 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRE             VLEETGFDV+  L
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRELSSAILLVNVAFQVLEETGFDVSKLL 180

Query: 181 IKEDFIEVMFGQQRVRLYIIAGVKNDTAFAPLTKKEISEIAWHRLDDLLPVSDDVISHGI 240
            +E++IE +F QQRVRLYI+AGV  DT FAPLTKKEISEI WHRLD L P S++VI+HG+
Sbjct: 181 KREEYIEFVFRQQRVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGV 240

Query: 241 TGLKLYMVAPFLESLRSWILKHQPPVAPNFDMPVKGFTMWKVKNTSMSNSSIIFDNQ 283
           +GLKLYMVAPFL SL+SWILKH  PVA   + P+K   +W  + +   N +   ++Q
Sbjct: 241 SGLKLYMVAPFLSSLKSWILKHPSPVARRPNKPLKALCVWNARTSVGGNGTATVESQ 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GW311.1e-11972.18mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=... [more]
O138283.2e-5347.37mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q9CYC64.6e-5242.42m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2[more]
Q8IU607.8e-5241.56m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3[more]
Q5REQ87.8e-5241.56m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KWT49.8e-183100.00Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G62... [more]
A0A5D3CCQ61.1e-17898.09mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BG591.1e-17898.09mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103489466 PE=... [more]
A0A6J1CC632.4e-16591.08mRNA-decapping enzyme subunit 2-like OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1IK277.1e-16591.08mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477819... [more]
Match NameE-valueIdentityDescription
XP_004142410.12.0e-182100.00mRNA-decapping enzyme subunit 2 [Cucumis sativus] >XP_031742317.1 mRNA-decapping... [more]
XP_008446886.12.3e-17898.09PREDICTED: mRNA-decapping enzyme subunit 2-like [Cucumis melo] >KAA0034722.1 mRN... [more]
XP_038891421.12.8e-17697.45mRNA-decapping enzyme subunit 2-like isoform X2 [Benincasa hispida][more]
XP_038891420.11.9e-17295.89mRNA-decapping enzyme subunit 2-like isoform X1 [Benincasa hispida][more]
XP_022139266.15.0e-16591.08mRNA-decapping enzyme subunit 2-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G13570.18.1e-12172.18decapping 2 [more]
AT5G13570.24.9e-11869.02decapping 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007722mRNA decapping protein 2, Box A domainSMARTSM01125DCP2_2coord: 19..103
e-value: 2.9E-43
score: 159.7
IPR007722mRNA decapping protein 2, Box A domainPFAMPF05026DCP2coord: 20..102
e-value: 2.1E-28
score: 98.4
IPR036189mRNA decapping protein 2, Box A domain superfamilyGENE3D1.10.10.1050Dcp2, box A domaincoord: 21..104
e-value: 3.7E-86
score: 290.0
IPR036189mRNA decapping protein 2, Box A domain superfamilySUPERFAMILY140586Dcp2 domain-likecoord: 14..103
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 105..246
e-value: 3.7E-86
score: 290.0
NoneNo IPR availablePANTHERPTHR23114M7GPPPN-MRNA HYDROLASEcoord: 13..266
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 107..214
e-value: 9.5E-13
score: 48.3
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 104..233
score: 13.296632
IPR020084NUDIX hydrolase, conserved sitePROSITEPS00893NUDIX_BOXcoord: 137..158
IPR044099mRNA decapping enzyme 2 , NUDIX hydrolase domainCDDcd03672Dcp2pcoord: 106..248
e-value: 7.10859E-74
score: 222.113
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 106..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G27150.1CSPI05G27150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA
biological_process GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050072 m7G(5')pppN diphosphatase activity
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0016787 hydrolase activity