CSPI05G26810 (gene) Cucumber (PI 183967) v1

BLAST of CSPI05G26810 vs. ExPASy Swiss-Prot
Match: G7L0H3 (Blue copper protein 1a OS=Medicago truncatula OX=3880 GN=MTR_7g086140 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 6.5e-32
Identity = 74/152 (48.68%), Postives = 103/152 (67.76%), Query Frame = 0

Query: 1   MAVVMYAPSALATNYTVGDDAGWSINVNYTLWAQGKMFNVGDMLIFNYPPGDHNVFKVNG 60
           +++V+ +  A+AT++ VGDD GW+++ +YT WAQ K+F VGD L+FNY P  HNVFKVNG
Sbjct: 12  ISMVLLSSVAIATDHIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNYDPARHNVFKVNG 71

Query: 61  SDFQNCTLPKDGQNALTSGSDVIVLAKPGKKWYICGKEGHCGQGQ-KLVINVMDMGPANS 120
           + FQ+CT P   + AL++G D+I L   G+KWY+CG   HC   Q KLVI V+  G   +
Sbjct: 72  TLFQSCTFPPKNE-ALSTGKDIIQLKTEGRKWYVCGVADHCSARQMKLVITVLAEG---A 131

Query: 121 PLPGGTAPPPPSAATKAVVSAQFGFV-ALVVA 151
           P P     PPPS+   +VVS+ FG V A++VA
Sbjct: 132 PAPS----PPPSSDAHSVVSSLFGVVMAIMVA 155

BLAST of CSPI05G26810 vs. ExPASy Swiss-Prot
Match: A0A072U307 (Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.5e-31
Identity = 74/150 (49.33%), Postives = 100/150 (66.67%), Query Frame = 0

Query: 3   VVMYAPSALATNYTVGDDAGWSINVNYTLWAQGKMFNVGDMLIFNYPPGDHNVFKVNGSD 62
           V++ + +  AT+Y VGDD GW+++ +YT WAQ K+F VGD L+FNY P  HNVFKVNG+ 
Sbjct: 15  VLLSSVAIAATDYIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNYDPSRHNVFKVNGTL 74

Query: 63  FQNCTLPKDGQNALTSGSDVIVLAKPGKKWYICGKEGHCGQGQ-KLVINVMDMGPANSPL 122
           FQ+CT P   + AL++G D+I L   G+KWY+CG   HC   Q KLVI V+  G   +P 
Sbjct: 75  FQSCTFPPKNE-ALSTGKDIIQLKTEGRKWYVCGVADHCSARQMKLVITVLAEG---APA 134

Query: 123 PGGTAPPPPSAATKAVVSAQFGFV-ALVVA 151
           P     PPPS+   +VVS+ FG V A++VA
Sbjct: 135 PS----PPPSSDAHSVVSSLFGVVMAIMVA 156

BLAST of CSPI05G26810 vs. ExPASy Swiss-Prot
Match: A0A0M4FTF3 (Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 6.8e-29
Identity = 63/123 (51.22%), Postives = 77/123 (62.60%), Query Frame = 0

Query: 7   APSALATNYTVGDDAGWSINVNYTLWAQGKMFNVGDMLIFNYPPGDHNVFKVNGSDFQNC 66
           A  A  T Y VGDD GW+I+V+Y  WA+GK F VGD L+F Y  G HNVFKVN + FQNC
Sbjct: 110 ASPAPQTEYWVGDDKGWTIDVDYQAWAKGKTFKVGDTLVFKYTKGHHNVFKVNQTGFQNC 169

Query: 67  TLPKDGQNALTSGSDVIVLAKPGKKWYICGKEGHCGQGQKLVINVMDMGPANSPLPGGTA 126
             P   +  LTSG DVI LA PGKKWYICG   HC + ++ +   ++  PA +P     A
Sbjct: 170 IAPPPSE-GLTSGHDVITLAAPGKKWYICGFPTHCSEHKQKLAITVEGAPAQTPPVWAPA 229

Query: 127 PPP 130
           P P
Sbjct: 230 PAP 231

BLAST of CSPI05G26810 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 8.3e-19
Identity = 46/127 (36.22%), Postives = 72/127 (56.69%), Query Frame = 0

Query: 11  LATNYTVGDDAGWSINVNYTLWAQGKMFNVGDMLIFNYPPGDHNVFKVNGSDFQNCTLPK 70
           +AT++T+G  +GW++  +   WA G+ F VGD L+F+YP   H+V +V   +F +C   K
Sbjct: 22  VATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYPAAFHDVVEVTKPEFDSCQAVK 81

Query: 71  DGQNALTSGSDVIVLAKPGKKWYICGKEGHCGQGQKLVINVM------DMGPANSPLPGG 130
                  +G+ ++ L  PGK+++ICG  GHC QG KL +NV+         P  + +P  
Sbjct: 82  P-LITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVVPTATVAPTAPLPNTVPSL 141

Query: 131 TAPPPPS 132
            AP P S
Sbjct: 142 NAPSPSS 147

BLAST of CSPI05G26810 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.8e-18
Identity = 58/145 (40.00%), Postives = 78/145 (53.79%), Query Frame = 0

Query: 1   MAVVMYAPSALATNYTVGDDAGWSINVNYTLWAQGKMFNVGDMLIFNYPPGDHNVFKVNG 60
           +A++  A  +LAT YTVGD +GW I  +Y+ WA  K F VGD L+FNY  G H V +V  
Sbjct: 13  LAIINMALPSLATVYTVGDTSGWVIGGDYSTWASDKTFAVGDSLVFNYGAGAHTVDEVKE 72

Query: 61  SDFQNCTLPKDGQNALT--SGSDVIVLAKPGKKWYICGKEGHCGQGQKLVINV-----MD 120
           SD+++CT    G +  T  +G+  I L K GK ++ICG  GH   G KL I V       
Sbjct: 73  SDYKSCT---SGNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSIKVKASSGSS 132

Query: 121 MGPANSPLPGGTAPP----PPSAAT 135
             P+ +P   G   P     P+A T
Sbjct: 133 AAPSATPSSSGKGSPSSDDTPAATT 154

BLAST of CSPI05G26810 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 8.9e-242
Identity = 429/513 (83.63%), Postives = 467/513 (91.03%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGANVG+LSGL+N+VTPPWVVLSIGA++NFFGYFMIWLA+T RISTPKVWQMCLYICIG
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIG 129

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQSF+NTGS+VTCVKNFP+SRG VLGILKGYVGLSGAIITQLFHAFYG D KSLIL I
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLI 189

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
            WLP AIS ASL  IRIMK IRQPNELKVFYNFLYISLALAGFLML+IIVESK +F QN+
Sbjct: 190 GWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKKEFNQNE 249

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEHPKQ-EQK 390
           YGGS AVVLLLL LPL +V++EEYNLWKLKT L+KS NPSVQIVTE+LPKTEHPKQ EQK
Sbjct: 250 YGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKTEHPKQLEQK 309

Query: 391 EPSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQIGMSLGYP 450
           EPSCW TIF+PP+RGEDFTILQ LFS DMLILF +  CG+GGTLTAIDNLGQIGMSLGYP
Sbjct: 310 EPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLGQIGMSLGYP 369

Query: 451 KKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGHLMIAFDVP 510
           K+SIS FVTLVSIW+YLGRV  GF SEIVL KYKFPR LILSL LLLSCVGHL+IAFDVP
Sbjct: 370 KRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLIIAFDVP 429

Query: 511 NGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVKVAGNF 570
           NGLY ASIVIGFCFGAQWPL+FAIISELFGLKYY+TL+ FG+VASP+GLY+LNVKVAGNF
Sbjct: 430 NGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNF 489

Query: 571 YDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYK 630
           YDREA KQL+ KGI+RKAG+ELKC+GGECFKLSFIVITAVTL+GM +SL+LVIRTRSFYK
Sbjct: 490 YDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVSLVLVIRTRSFYK 549

Query: 631 SDIYKKFRDEAET-EVAGNEAVATAGAVEETEK 662
           SDIYKKFRDEAET EVAGN  +  AG  EE  K
Sbjct: 550 SDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

BLAST of CSPI05G26810 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 8.9e-242
Identity = 429/513 (83.63%), Postives = 467/513 (91.03%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGANVG+LSGL+N+VTPPWVVLSIGA++NFFGYFMIWLA+T RISTPKVWQMCLYICIG
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIG 129

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQSF+NTGS+VTCVKNFP+SRG VLGILKGYVGLSGAIITQLFHAFYG D KSLIL I
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLI 189

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
            WLP AIS ASL  IRIMK IRQPNELKVFYNFLYISLALAGFLML+IIVESK +F QN+
Sbjct: 190 GWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKKEFNQNE 249

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEHPKQ-EQK 390
           YGGS AVVLLLL LPL +V++EEYNLWKLKT L+KS NPSVQIVTE+LPKTEHPKQ EQK
Sbjct: 250 YGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKTEHPKQLEQK 309

Query: 391 EPSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQIGMSLGYP 450
           EPSCW TIF+PP+RGEDFTILQ LFS DMLILF +  CG+GGTLTAIDNLGQIGMSLGYP
Sbjct: 310 EPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLGQIGMSLGYP 369

Query: 451 KKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGHLMIAFDVP 510
           K+SIS FVTLVSIW+YLGRV  GF SEIVL KYKFPR LILSL LLLSCVGHL+IAFDVP
Sbjct: 370 KRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLIIAFDVP 429

Query: 511 NGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVKVAGNF 570
           NGLY ASIVIGFCFGAQWPL+FAIISELFGLKYY+TL+ FG+VASP+GLY+LNVKVAGNF
Sbjct: 430 NGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNF 489

Query: 571 YDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYK 630
           YDREA KQL+ KGI+RKAG+ELKC+GGECFKLSFIVITAVTL+GM +SL+LVIRTRSFYK
Sbjct: 490 YDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVSLVLVIRTRSFYK 549

Query: 631 SDIYKKFRDEAET-EVAGNEAVATAGAVEETEK 662
           SDIYKKFRDEAET EVAGN  +  AG  EE  K
Sbjct: 550 SDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

BLAST of CSPI05G26810 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 4.7e-235
Identity = 421/520 (80.96%), Postives = 461/520 (88.65%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGANVG+LSGL+N+VTPPWVVLSIGA+MNFFGYFMIWLA+T +I+ PKVWQMCLYICIG
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVWQMCLYICIG 129

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQSF+NTGS+VTCVKNFP+SRG VLGILKGYVGLSGAIITQLFHAFYG D KSLIL I
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLI 189

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
            WLP AIS A L  IRIMK IRQPNELKVFYNFLYISLALAGFLML+IIVESKT+F+Q+ 
Sbjct: 190 GWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKTEFSQSG 249

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKLK----TTLIKSSNPSVQIVTEQLPKTEHPK- 390
           YGGS AVVLLLL LPL +V++EEYNLW LK    T  I+S N  VQIVTE+LPKTEH K 
Sbjct: 250 YGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEKLPKTEHQKH 309

Query: 391 ----QEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQ 450
               + QKEPSCW TIF+PPERGEDFTILQ LFSVDMLILF +  CG+GGTLTAIDNLGQ
Sbjct: 310 IPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGTLTAIDNLGQ 369

Query: 451 IGMSLGYPKKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGH 510
           IGMSLGYPK+SIS FVTLVSIW+YLGRV  GF SEIVL KYKFPR L+LS+ LL+SCVGH
Sbjct: 370 IGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLVSCVGH 429

Query: 511 LMIAFDVPNGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVL 570
           LMIAFDVPNGLY ASIVIGFCFGAQWPL+FAIISELFGLKYY+TL+ FG+VASP+GLYVL
Sbjct: 430 LMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVL 489

Query: 571 NVKVAGNFYDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILV 630
           NVKVAGNFYDREA KQL  KGI+RK G+ELKC+GGECFKLSF+VIT VTL+GMF+SLILV
Sbjct: 490 NVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLGMFVSLILV 549

Query: 631 IRTRSFYKSDIYKKFRDEAETEVAGNEAVATAGAVEETEK 662
           IRTRSFYKSDIYKKFRDEAETE+AGN AV  AG  EET K
Sbjct: 550 IRTRSFYKSDIYKKFRDEAETEMAGNGAVVAAGPPEETRK 589

BLAST of CSPI05G26810 vs. ExPASy TrEMBL
Match: A0A5D3CCM6 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002390 PE=4 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 2.0e-233
Identity = 417/466 (89.48%), Postives = 442/466 (94.85%), Query Frame = 0

Query: 203 MCLYICIGANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGAD 262
           MCLYICIGANSQSF+NTGS+VTCVKNFPQSRGA+LGILKGY+GLSGAIITQLFHAFYGAD
Sbjct: 1   MCLYICIGANSQSFANTGSMVTCVKNFPQSRGALLGILKGYIGLSGAIITQLFHAFYGAD 60

Query: 263 EKSLILFIAWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVES 322
            KSLILFI WLP A+S ASLPIIRIMK IRQPNELKVFYNFLYISLALAGFLML+IIVES
Sbjct: 61  AKSLILFIGWLPPAVSFASLPIIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVES 120

Query: 323 KTKFTQNQYGGSVAVVLLLLLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTE 382
           KT+FTQNQYGGSVAV+LLLLLLP+V+VV+EEYNLWKLKT+L+KS  PSVQIVTEQLPKTE
Sbjct: 121 KTEFTQNQYGGSVAVLLLLLLLPIVVVVIEEYNLWKLKTSLVKSPTPSVQIVTEQLPKTE 180

Query: 383 HPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQI 442
           HPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLI+FTS ACGLGGTLTAIDN GQI
Sbjct: 181 HPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLIVFTSAACGLGGTLTAIDNFGQI 240

Query: 443 GMSLGYPKKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGHL 502
           GMSLGYPKK IS FVTLVSIW+YLGRV CGFFSEIVLIKYKFPRTLI SLILLLSCVGHL
Sbjct: 241 GMSLGYPKKCISTFVTLVSIWNYLGRVACGFFSEIVLIKYKFPRTLIFSLILLLSCVGHL 300

Query: 503 MIAFDVPNGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLN 562
           MIAFDVPNGLY ASIVIGFCFGAQWPL+FAIIS+LFGLKYY+TL+ FG+VASP+GLYVLN
Sbjct: 301 MIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISDLFGLKYYSTLYNFGSVASPIGLYVLN 360

Query: 563 VKVAGNFYDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILVI 622
           VKVAGNFYDREA KQL  +GI+RKAG+ELKCYGGECFKLSFIVITAV+LMGMFISLILVI
Sbjct: 361 VKVAGNFYDREAEKQLRARGIIRKAGEELKCYGGECFKLSFIVITAVSLMGMFISLILVI 420

Query: 623 RTRSFYKSDIYKKFRDEAETEVAGNEAVATAGAVEETEKTIIQRGS 669
           RTRSFYKSDIYKKFRDEAETEVAGNEA AT GA E TEKTII R S
Sbjct: 421 RTRSFYKSDIYKKFRDEAETEVAGNEAAATTGAAEVTEKTIIHRRS 466

BLAST of CSPI05G26810 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 5.8e-233
Identity = 416/516 (80.62%), Postives = 457/516 (88.57%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGANVG+LSGL+N+VTPPWVVLSIGA+MNFFGYFMIWLA+T +IS PKVWQMCLYICIG
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTGKISAPKVWQMCLYICIG 129

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQSF+NTGS+VTCVKNFP+SRG VLGILKGYVGLSGAIITQLFHAFYG D KSLIL I
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLI 189

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
            WLP AIS A L  IRIMK IRQPNELKVFYNFLYISLALAGFLML+II+ESKT+F+Q+ 
Sbjct: 190 GWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKTEFSQSG 249

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKLKT-----TLIKSSNPSVQIVTEQLPKTEHPK 390
           YGGS AVVLLLL LPL +V++EEYNLW LKT       I+S N  VQIVTE++PKTEH  
Sbjct: 250 YGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAAIRSPNHPVQIVTEKVPKTEH-- 309

Query: 391 QEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQIGMS 450
             QK+PSCW TIF+PPERGEDFTILQ LFSVDM ILF +  CG+GGTLTAIDNLGQIGMS
Sbjct: 310 --QKQPSCWTTIFNPPERGEDFTILQALFSVDMFILFLAAICGVGGTLTAIDNLGQIGMS 369

Query: 451 LGYPKKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGHLMIA 510
           LGYPK+SIS FVTLVSIW+YLGRV  GF SEIVL KYKFPR L+LS+ LL+SCVGHLMIA
Sbjct: 370 LGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLVSCVGHLMIA 429

Query: 511 FDVPNGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVKV 570
           FDVPNGLY ASIVIGFCFGAQWPL+FAIISELFGLKYY+TL+ FG+VASP+GLYVLNVKV
Sbjct: 430 FDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKV 489

Query: 571 AGNFYDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILVIRTR 630
           AGNFYDREA KQL  KGI+RK G+ELKC+GGECFKLSF+VIT VTL+GMF+SLILVIRTR
Sbjct: 490 AGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLGMFVSLILVIRTR 549

Query: 631 SFYKSDIYKKFRDEAETEVAGNEAVATAGAVEETEK 662
           SFYKSDIYKKFRDEAETE+AGN AV  A   EET K
Sbjct: 550 SFYKSDIYKKFRDEAETEIAGNGAVVAAEPPEETRK 581

BLAST of CSPI05G26810 vs. NCBI nr
Match: XP_011655862.2 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KAE8648847.1 hypothetical protein Csa_008229 [Cucumis sativus])

HSP 1 Score: 976.5 bits (2523), Expect = 1.2e-280
Identity = 508/518 (98.07%), Postives = 508/518 (98.07%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG
Sbjct: 70  DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 129

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI
Sbjct: 130 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 189

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
           AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ
Sbjct: 190 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 249

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEHPKQEQKE 390
           YGG VAVV   LLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEHPKQEQKE
Sbjct: 250 YGGIVAVV---LLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEHPKQEQKE 309

Query: 391 PSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQIGMSLGYPK 450
           PSCWRTIFSPPERGEDFTILQGLFSVDMLIL TSVACGLGGTLTAIDNLGQIGMSLGYPK
Sbjct: 310 PSCWRTIFSPPERGEDFTILQGLFSVDMLILLTSVACGLGGTLTAIDNLGQIGMSLGYPK 369

Query: 451 KSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGHLMIAFDVPN 510
           KSISIFVTLVSIWSYLGRVGCGF SEIVLIKYKFPRTLILSLILLLSCVGHLMIAFDVPN
Sbjct: 370 KSISIFVTLVSIWSYLGRVGCGFISEIVLIKYKFPRTLILSLILLLSCVGHLMIAFDVPN 429

Query: 511 GLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVKVAGNFY 570
           GLY ASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVKVAGNFY
Sbjct: 430 GLYVASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVKVAGNFY 489

Query: 571 DREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKS 630
           DREARKQLNTKGILRKAGKEL CYGGECFKLSF VITAVTLMGMFISLILVIRTRSFYKS
Sbjct: 490 DREARKQLNTKGILRKAGKELNCYGGECFKLSFTVITAVTLMGMFISLILVIRTRSFYKS 549

Query: 631 DIYKKFRDEAETEVAGNEAVATAGAVEETEKTIIQRGS 669
           DIYKKFRDEAETEVAGNEAVATAGAVEETEKTIIQR S
Sbjct: 550 DIYKKFRDEAETEVAGNEAVATAGAVEETEKTIIQRRS 584

BLAST of CSPI05G26810 vs. NCBI nr
Match: XP_004142500.2 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8648848.1 hypothetical protein Csa_009434 [Cucumis sativus])

HSP 1 Score: 869.4 bits (2245), Expect = 2.0e-248
Identity = 442/512 (86.33%), Postives = 473/512 (92.38%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGAN+G+LSGL+N+VTPPWVVLSIGA+MNFFGYFMIWLAITNRISTPKVWQMCLYICIG
Sbjct: 70  DLGANIGVLSGLINEVTPPWVVLSIGALMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 129

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQSF+NTGS+VTCV NFP+SRG VLGILKGY GLSGAIITQLFHAFYGAD KSL+LFI
Sbjct: 130 ANSQSFANTGSMVTCVMNFPESRGVVLGILKGYAGLSGAIITQLFHAFYGADTKSLVLFI 189

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
            WLPTA+S ASL IIRI+K IRQPNELKVFYNFLYISLALAGFLML+IIVESKT+FTQNQ
Sbjct: 190 GWLPTAVSFASLRIIRIIKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKTEFTQNQ 249

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEHPKQEQKE 390
           YGGS AVVLLLLLLPL+IVVMEEY LWKLKT LI+S NPSVQIVTEQLPKTEHPKQE KE
Sbjct: 250 YGGSAAVVLLLLLLPLLIVVMEEYKLWKLKTALIQSPNPSVQIVTEQLPKTEHPKQEHKE 309

Query: 391 PSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQIGMSLGYPK 450
           PSCW TIFSPP+RGEDFTILQGLFSVDMLILFTS ACG+GGTLTAIDNLGQIG+SLGYPK
Sbjct: 310 PSCWTTIFSPPKRGEDFTILQGLFSVDMLILFTSAACGMGGTLTAIDNLGQIGVSLGYPK 369

Query: 451 KSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGHLMIAFDVPN 510
           +SIS FVTLVSIW+YLGRV CGF SEIVL KYK PRTLILSLILLLSCVGHLMIAFDVPN
Sbjct: 370 RSISTFVTLVSIWNYLGRVACGFLSEIVLRKYKCPRTLILSLILLLSCVGHLMIAFDVPN 429

Query: 511 GLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVKVAGNFY 570
           GLY ASIVIGFCFGAQWPL+FAIISELFGLKYY+TL+ FG+VASP+GLYVLNVKVAGNFY
Sbjct: 430 GLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFY 489

Query: 571 DREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKS 630
           DREA KQL  KGI+RKAG+ELKC GG CFKLSFIVIT VTL+GM +SLILVIRTRSFY+S
Sbjct: 490 DREAEKQLEAKGIIRKAGEELKCIGGACFKLSFIVITGVTLLGMLVSLILVIRTRSFYRS 549

Query: 631 DIYKKFRDEAE-TEVAGNEAVATAGAVEETEK 662
           DIYKKFR+E E TEVAGN  V  AG  EE  K
Sbjct: 550 DIYKKFREEVETTEVAGNRVVEAAGETEERRK 581

BLAST of CSPI05G26810 vs. NCBI nr
Match: XP_038893353.1 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 850.1 bits (2195), Expect = 1.3e-242
Identity = 429/515 (83.30%), Postives = 468/515 (90.87%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGANVG+LSGL+N+VTPPWVVLSIGAI+NFFGYFMIWLA+T RIS PKVWQMC+YICIG
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVWQMCVYICIG 129

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQSF+NTGS+VTCVKNFP+SRG VLGILKGYVGLSGAIITQLFHAFYG D KSLIL I
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLI 189

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
            WLP AIS A L  IRI+K IR PNELKVFYNFLYISL LAGFLML+II+ESKT+FTQN+
Sbjct: 190 GWLPAAISFAFLRTIRIIKVIRHPNELKVFYNFLYISLGLAGFLMLMIIIESKTEFTQNE 249

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEH----PKQ 390
           YGGS AVVL+LL LPL +V++EEYNLWKLKT  IKSSNP VQIVTEQLPKTEH    PKQ
Sbjct: 250 YGGSAAVVLVLLFLPLAVVIIEEYNLWKLKTAFIKSSNPCVQIVTEQLPKTEHPKQTPKQ 309

Query: 391 EQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQIGMSL 450
           EQKEPSCW T+FSPPERGEDFTILQ LFS+DMLILF S  CG+GGTLTAIDNLGQIGMSL
Sbjct: 310 EQKEPSCWTTVFSPPERGEDFTILQALFSIDMLILFLSATCGVGGTLTAIDNLGQIGMSL 369

Query: 451 GYPKKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGHLMIAF 510
           GYPK+SIS FV+LVSIW+YLGR+  GF SEIV+ KYKFPR LILSL LLLSCVGHLMIAF
Sbjct: 370 GYPKRSISTFVSLVSIWNYLGRIASGFVSEIVVTKYKFPRPLILSLTLLLSCVGHLMIAF 429

Query: 511 DVPNGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVKVA 570
           DVPNGLY AS+VIGFCFGAQWP++FAIISELFGLKYY+TL+ FG+VASP+GLY+LNVKVA
Sbjct: 430 DVPNGLYVASVVIGFCFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVA 489

Query: 571 GNFYDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRS 630
           GNFYDREA KQL  KGI RKAGK+LKC+GGECFKLSFIVITAVTL+G FISLILVIRT+S
Sbjct: 490 GNFYDREAEKQLRAKGITRKAGKKLKCFGGECFKLSFIVITAVTLLGTFISLILVIRTKS 549

Query: 631 FYKSDIYKKFRDEAETEVAGNEAVATAGAVEETEK 662
           FYKSDIYKKFRDEAETEVAGN AVA AGA EET+K
Sbjct: 550 FYKSDIYKKFRDEAETEVAGNGAVAPAGAGEETKK 584

BLAST of CSPI05G26810 vs. NCBI nr
Match: XP_008446819.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0034695.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK09246.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 846.3 bits (2185), Expect = 1.8e-241
Identity = 429/513 (83.63%), Postives = 467/513 (91.03%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGANVG+LSGL+N+VTPPWVVLSIGA++NFFGYFMIWLA+T RISTPKVWQMCLYICIG
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIG 129

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQSF+NTGS+VTCVKNFP+SRG VLGILKGYVGLSGAIITQLFHAFYG D KSLIL I
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLI 189

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
            WLP AIS ASL  IRIMK IRQPNELKVFYNFLYISLALAGFLML+IIVESK +F QN+
Sbjct: 190 GWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKKEFNQNE 249

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEHPKQ-EQK 390
           YGGS AVVLLLL LPL +V++EEYNLWKLKT L+KS NPSVQIVTE+LPKTEHPKQ EQK
Sbjct: 250 YGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKTEHPKQLEQK 309

Query: 391 EPSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQIGMSLGYP 450
           EPSCW TIF+PP+RGEDFTILQ LFS DMLILF +  CG+GGTLTAIDNLGQIGMSLGYP
Sbjct: 310 EPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLGQIGMSLGYP 369

Query: 451 KKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGHLMIAFDVP 510
           K+SIS FVTLVSIW+YLGRV  GF SEIVL KYKFPR LILSL LLLSCVGHL+IAFDVP
Sbjct: 370 KRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLIIAFDVP 429

Query: 511 NGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVKVAGNF 570
           NGLY ASIVIGFCFGAQWPL+FAIISELFGLKYY+TL+ FG+VASP+GLY+LNVKVAGNF
Sbjct: 430 NGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNF 489

Query: 571 YDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYK 630
           YDREA KQL+ KGI+RKAG+ELKC+GGECFKLSFIVITAVTL+GM +SL+LVIRTRSFYK
Sbjct: 490 YDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVSLVLVIRTRSFYK 549

Query: 631 SDIYKKFRDEAET-EVAGNEAVATAGAVEETEK 662
           SDIYKKFRDEAET EVAGN  +  AG  EE  K
Sbjct: 550 SDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

BLAST of CSPI05G26810 vs. NCBI nr
Match: XP_004142499.3 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8648849.1 hypothetical protein Csa_008497 [Cucumis sativus])

HSP 1 Score: 843.2 bits (2177), Expect = 1.6e-240
Identity = 427/512 (83.40%), Postives = 464/512 (90.62%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGANVG+LSGL+N+VTPPWVVLSIGA++NFFGYFMIWLA+T RIS PKVWQMCLYICIG
Sbjct: 70  DLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVWQMCLYICIG 129

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQSF+NTGS+VTCVKNFP+SRG VLGILKGYVGLSGAIITQLFHAFYG D KSLIL I
Sbjct: 130 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGDDTKSLILLI 189

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
            WLP AIS ASL  IRIMK IRQPNELKVFYNFLYISLALAGFLML+IIVESK +F QN+
Sbjct: 190 GWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKKQFNQNE 249

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEHPKQEQKE 390
           YGGS AVVLLLL+LPL +V++EEYNLWKLKT +IKS NPSVQIVTE+LPKTEHPKQE+KE
Sbjct: 250 YGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKTEHPKQERKE 309

Query: 391 PSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQIGMSLGYPK 450
           PSCW TIFSPP+RGEDFTILQ LFSVDMLILF +  CG+GGTLTAIDNLGQIG++LGYPK
Sbjct: 310 PSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLGQIGLALGYPK 369

Query: 451 KSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGHLMIAFDVPN 510
           +SIS FV+LVSIW+YLGRV  GF SEIVL KYKFPR LILSL LLLSCVGHLMIAFDVPN
Sbjct: 370 RSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLMIAFDVPN 429

Query: 511 GLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVKVAGNFY 570
           GLY ASIVIGFCFGAQWPL+FAIISELFGLKYY+TL+ FG+VASP+GLYVLNVKVAGNFY
Sbjct: 430 GLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFY 489

Query: 571 DREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILVIRTRSFYKS 630
           DREA KQL  K I+RKAG+ELKC+GGECFKLSFIVIT VTL+GM +SLILVIRTRSFYKS
Sbjct: 490 DREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLILVIRTRSFYKS 549

Query: 631 DIYKKFRDEAE-TEVAGNEAVATAGAVEETEK 662
           DIYKKFRDE E TEVAGN  V  AG  EE  K
Sbjct: 550 DIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 581

BLAST of CSPI05G26810 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 665.6 bits (1716), Expect = 4.1e-191
Identity = 342/526 (65.02%), Postives = 416/526 (79.09%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGANVG+L+GL+N+VTPPW +L IGAI+NFFGYFMIWLA+T RIS P+VW MCLYIC+G
Sbjct: 67  DLGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVG 126

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQSF+NTGS+VTCVKNFP+SRG VLGILKGYVGLSGAIITQL+ AFYG D K LIL I
Sbjct: 127 ANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMI 186

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
            WLP  +S A L  IRIMK  RQ NELKVFYNFLYISL LA FLM+VII+   + FTQ++
Sbjct: 187 GWLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSE 246

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEHPK----- 390
           +GGS AVV++LLLLP+++V++EE  LWK K   +    P + +VTE+ PK +  +     
Sbjct: 247 FGGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAP-INVVTEK-PKLDSSEFKDDD 306

Query: 391 -QEQKE-------PSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAID 450
            +E KE       PSCW T+F+PPERG+D+TILQ LFSVDMLILF +  CG+GGTLTAID
Sbjct: 307 GEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAID 366

Query: 451 NLGQIGMSLGYPKKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLS 510
           NLGQIG SLGYPK+S+S FV+LVSIW+Y GRV  G  SEI LIKYKFPR L+L+++LLLS
Sbjct: 367 NLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLS 426

Query: 511 CVGHLMIAFDVPNGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVG 570
           C GHL+IAF+VP GLY AS++IGFCFGAQWPL+FAIISE+FGLKYY+TL+ FG+VASP+G
Sbjct: 427 CAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIG 486

Query: 571 LYVLNVKVAGNFYDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFIS 630
            Y+LNV+VAG  YD EA KQ    G  R  G++L C G  CFKLSFI+I AVTL G+ +S
Sbjct: 487 SYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVS 546

Query: 631 LILVIRTRSFYKSDIYKKFRDE---AETEVAGNEAVATAGAVEETE 661
           ++LVIRT+ FYKSDIYKKFR++   AE E+A   A  +  A E+ +
Sbjct: 547 MVLVIRTKKFYKSDIYKKFREKALAAEMEMAAPAAARSTVAKEDKD 590

BLAST of CSPI05G26810 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 571.2 bits (1471), Expect = 1.1e-162
Identity = 288/499 (57.72%), Postives = 368/499 (73.75%), Query Frame = 0

Query: 151 DLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYICIG 210
           DLGANVG+LSGL+ +VTP W VL+IG+ MNF GYFMIWL +T +++ PKVWQMCLYICIG
Sbjct: 63  DLGANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIG 122

Query: 211 ANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLILFI 270
           ANSQ+F+NTG++VTCVKNFP+SRG +LG+LKGYVGLSGAI TQL+ A YG D KSLIL I
Sbjct: 123 ANSQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLI 182

Query: 271 AWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVESKTKFTQNQ 330
           AWLP A+S   + +IR  K +RQ NEL VFY FLYIS+ LA FLM + I E +  F++  
Sbjct: 183 AWLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAA 242

Query: 331 YGGSVAVVLLLLLLPLVIVVMEEYNLWKL------KTTLIKSSNPSVQIVTEQLPKTEHP 390
           Y  S  +   LL +PL + V +E  +W +      + + +K   P  ++  +Q    +  
Sbjct: 243 YAASATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVN 302

Query: 391 KQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLGQIGM 450
            +E++  SC+ T+FSPP RGED+TILQ L S DM+ILF +  CGLG +LTA+DNLGQIG 
Sbjct: 303 GEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGE 362

Query: 451 SLGYPKKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVGHLMI 510
           SLGYP  ++S FV+LVSIW+Y GRV  GF SE +L KYK PR L+++L+LLLSC GHL+I
Sbjct: 363 SLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLI 422

Query: 511 AFDVPNGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYVLNVK 570
           AF VP  +Y ASI++GF FGAQ PL+FAIISELFGLKYY+TL   G +ASP+G Y+LNV+
Sbjct: 423 AFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVR 482

Query: 571 VAGNFYDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLILVIRT 630
           V G  YD+EA KQL  +G+ RK  K+L C G +C+KL F+++ AVT  G  +SL L IRT
Sbjct: 483 VTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRT 542

Query: 631 RSFYKSDIYKKFRDEAETE 644
           R FYK DIYKKFR+  E+E
Sbjct: 543 REFYKGDIYKKFRESPESE 561

BLAST of CSPI05G26810 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 285.4 bits (729), Expect = 1.2e-76
Identity = 173/506 (34.19%), Postives = 278/506 (54.94%), Query Frame = 0

Query: 148 VVADLGANVGILSGL----------------VNQVTPPWVVLSIGAIMNFFGYFMIWLAI 207
           V  D+GAN G+ SGL                +     PWVVL++GAI  F GYF+IW ++
Sbjct: 50  VFKDIGANAGVFSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASV 109

Query: 208 TNRISTPKVWQMCLYICIGANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAII 267
           T  I  P V  MCL++ + A SQ+F NT ++V+ V+NF    G  +GI+KG++GLSGAI+
Sbjct: 110 TGLIRKPPVPLMCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAIL 169

Query: 268 TQLFHAFYGADEKSLILFIAWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALA 327
            QL+      D  S IL +A  PT +S   +P++RI +     ++ K       +SL +A
Sbjct: 170 IQLYETLCAGDPASFILLLAVTPTVLSLLVMPLVRIYE-TSVADDKKHLNGLSAVSLIIA 229

Query: 328 GFLMLVIIVESKTKFTQNQYGGSVAVVLLLLLLPLVIVVMEEYNLWKLKTTLIKSSNPSV 387
            +LM++II+  K  F  + +   V +V LL++L L +++        ++ T+    +P +
Sbjct: 230 AYLMIIIIL--KNTFGLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPHDYSPLI 289

Query: 388 QIVTEQLPKTEHPKQEQKEPSCWRTIFSPPERG--EDFTILQGLFSVDMLILFTSVACGL 447
                  PK      +  E        S  E G  E+  +LQ +  +   +LF ++ CG+
Sbjct: 290 -----SSPKATTSGNQSSEGD------SKVEAGLSENLNLLQAMKKLSFWLLFLAMICGM 349

Query: 448 GGTLTAIDNLGQIGMSLGYPKKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLI 507
           G  L+ I+N+ QIG SL Y    I+  V+L SIW++LGR G G+ S+ +L K  +PR L+
Sbjct: 350 GSGLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLL 409

Query: 508 LSLILLLSCVGHLMIAFDVPNGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVF 567
           ++  L    +GHL+IA      LY  S+++G C+G+QW L+  I SELFG+++  T+   
Sbjct: 410 MAATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNT 469

Query: 568 GAVASPVGLYVLNVKVAGNFYDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAV 627
            +VASP+G Y+ +V++ G  YD+ A  + NT            CYG  CF+LSFI++ +V
Sbjct: 470 ISVASPIGSYIFSVRLIGYIYDKTASGEGNT------------CYGSHCFRLSFIIMASV 529

Query: 628 TLMGMFISLILVIRTRSFYKSDIYKK 636
              G  ++++L  RT++ Y+  + K+
Sbjct: 530 AFFGFLVAIVLFFRTKTLYRQILVKR 529

BLAST of CSPI05G26810 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 3.4e-76
Identity = 172/488 (35.25%), Postives = 285/488 (58.40%), Query Frame = 0

Query: 148 VVADLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYI 207
           V  +LG  +G +SG +++V+P WVVL +GA  N FGY ++WL +T ++    +W + + I
Sbjct: 60  VAKNLGDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAI 119

Query: 208 CIGANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLI 267
            +G N +++ NT S+V+C+ NFP+SRG V+GILKG+ GLSGAI+TQ++  F  + + S+I
Sbjct: 120 FVGTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVI 179

Query: 268 LFIAWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYI---SLALAGFLMLVIIVESKT 327
           L +A  P  +  A L I+R ++   + N       FL I    + LA +L+ +++++S  
Sbjct: 180 LMVALGPPVVVLALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVF 239

Query: 328 KFTQNQYGGSVAVVLLLLLLPLVI----VVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPK 387
             TQ     S A++++ +++P+++    V +   N+  +K     +SN         + +
Sbjct: 240 DMTQTIITTSGAILVIFMVVPVLVPFSSVFISGNNVTSVKPE-EGTSNVDQHEARTLIER 299

Query: 388 TEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLG 447
           ++ P ++++ P            GEDFT+LQ L   D  ++F S+  G+G  +T IDNLG
Sbjct: 300 SDRPPEKKRAPCI----------GEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLG 359

Query: 448 QIGMSLGYPKKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVG 507
           QI  SLGY   +  IFV+L+SI ++LGRV  G+FSE+++ K   PRTL +S++  +  +G
Sbjct: 360 QICYSLGY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLG 419

Query: 508 HLMIAFDVPNGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYV 567
            +  A D P  +Y  +IVIG  +GA W +  A +S++FGLK + +L+ F   A P+G +V
Sbjct: 420 LIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFV 479

Query: 568 LNVKVAGNFYDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLIL 627
            +  +A N YD  ARKQ           + L C G  C+ ++  +++ + LM M +SL +
Sbjct: 480 FSGVIASNIYDYYARKQAGP----TTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSV 530

Query: 628 VIRTRSFY 629
           V RTR FY
Sbjct: 540 VYRTRKFY 530

BLAST of CSPI05G26810 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 3.4e-76
Identity = 172/488 (35.25%), Postives = 285/488 (58.40%), Query Frame = 0

Query: 148 VVADLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVWQMCLYI 207
           V  +LG  +G +SG +++V+P WVVL +GA  N FGY ++WL +T ++    +W + + I
Sbjct: 60  VAKNLGDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAI 119

Query: 208 CIGANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGADEKSLI 267
            +G N +++ NT S+V+C+ NFP+SRG V+GILKG+ GLSGAI+TQ++  F  + + S+I
Sbjct: 120 FVGTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVI 179

Query: 268 LFIAWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYI---SLALAGFLMLVIIVESKT 327
           L +A  P  +  A L I+R ++   + N       FL I    + LA +L+ +++++S  
Sbjct: 180 LMVALGPPVVVLALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVF 239

Query: 328 KFTQNQYGGSVAVVLLLLLLPLVI----VVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPK 387
             TQ     S A++++ +++P+++    V +   N+  +K     +SN         + +
Sbjct: 240 DMTQTIITTSGAILVIFMVVPVLVPFSSVFISGNNVTSVKPE-EGTSNVDQHEARTLIER 299

Query: 388 TEHPKQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLILFTSVACGLGGTLTAIDNLG 447
           ++ P ++++ P            GEDFT+LQ L   D  ++F S+  G+G  +T IDNLG
Sbjct: 300 SDRPPEKKRAPCI----------GEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLG 359

Query: 448 QIGMSLGYPKKSISIFVTLVSIWSYLGRVGCGFFSEIVLIKYKFPRTLILSLILLLSCVG 507
           QI  SLGY   +  IFV+L+SI ++LGRV  G+FSE+++ K   PRTL +S++  +  +G
Sbjct: 360 QICYSLGY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLG 419

Query: 508 HLMIAFDVPNGLYAASIVIGFCFGAQWPLVFAIISELFGLKYYATLHVFGAVASPVGLYV 567
            +  A D P  +Y  +IVIG  +GA W +  A +S++FGLK + +L+ F   A P+G +V
Sbjct: 420 LIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFV 479

Query: 568 LNVKVAGNFYDREARKQLNTKGILRKAGKELKCYGGECFKLSFIVITAVTLMGMFISLIL 627
            +  +A N YD  ARKQ           + L C G  C+ ++  +++ + LM M +SL +
Sbjct: 480 FSGVIASNIYDYYARKQAGP----TTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSV 530

Query: 628 VIRTRSFY 629
           V RTR FY
Sbjct: 540 VYRTRKFY 530