CSPI05G24830 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G24830
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionprotein MIZU-KUSSEI 1
LocationChr5: 24319552 .. 24320652 (+)
RNA-Seq ExpressionCSPI05G24830
SyntenyCSPI05G24830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATTTTGGAAAAGAGAGAGAGAGAGGAAAGAACAAAAGTGATGGGTGTGTATTTATTTGTCAGTTTTTATGTTTTTTTTTGGGATATTGTAATTAGGTAGGTCTGCTCACCTTCCTTTTTCCTCAGGATTCTCCCATGGCTACCTCCATCTCCTACAATCTCTGACATTTTTTCTTCAAATTCCCACTTCCCTATTTCTCCCATAACATATCTCTCTTCTCTTTTAATATCACTTACTTCCTTTATACTTTCTCTCTCACTCATTTCTCTTTTCTCTTTCAAATTTACACACCACACTACCATGTCCAAATCCCTCCATGACTCTTCCTTCTCTTTCTCAAGGCGTTACTTCAACTGGAAGAACAAATTTCACGAAGAAGATGAACAGCAACAACAACACCAACAAATCTCATCTTTCAGTTTATCCTCTCGTTTCACTCAAGAAGACAGAGAACGCGACCTCAAAACCAAAACCCACATCAAACCTTCTGCCTCTTCCTCTTCCTCTTCCTCATCCATTCGTCATGTTTCCAAGCTCCGAACTGCTTTAACAGTCTTCTCCCGGATCCGACCAACCCACCACCGCTCCCGCCTCGGCGGCCGGGTCATCGGTACACTCTATGGCTACCGACGAGGCCACGTTTATTTCGCATTACAAGAAGACCCAAAACAGAGTCCAACGTTTTTAATCGAGCTCTCGACACCAACCAGCGTTCTGGTTCGAGAAATGGCATCTGGGTTGGTACGGATCGCGTTGGAATGTGAGAAGAAAACAGAGAGGAAGAAGAATTGCAAGTTAGTGGAAGAAGCACTTTGGAGAACGTATTGTAATGGGAAGAAATGTGGGTATGGGAGTAGAAGAGAATGTGGAACAGAGGAGCAGAAGATTTTGAAGGCGGTGGAGCCGATCACGATGGGGGCTGGGGTCTTGCCCCCAGGGCATGGTTCGGATGAGGAATTAATGTATATGAGAGCTAGATTTGAGAGGGTAATTGGGTCGAAGGATTCTGAAGCTTATTACATGATGAGCCCAGATTGTAATGCAGGGCCTGAACTCAGCATTTACTTGCTTAGGGTTTGAACTTTTGATCCCCCCCCCC

mRNA sequence

GTATTTTGGAAAAGAGAGAGAGAGAGGAAAGAACAAAAGTGATGGGTGTGTATTTATTTGTCAGTTTTTATGTTTTTTTTTGGGATATTGTAATTAGGTAGGTCTGCTCACCTTCCTTTTTCCTCAGGATTCTCCCATGGCTACCTCCATCTCCTACAATCTCTGACATTTTTTCTTCAAATTCCCACTTCCCTATTTCTCCCATAACATATCTCTCTTCTCTTTTAATATCACTTACTTCCTTTATACTTTCTCTCTCACTCATTTCTCTTTTCTCTTTCAAATTTACACACCACACTACCATGTCCAAATCCCTCCATGACTCTTCCTTCTCTTTCTCAAGGCGTTACTTCAACTGGAAGAACAAATTTCACGAAGAAGATGAACAGCAACAACAACACCAACAAATCTCATCTTTCAGTTTATCCTCTCGTTTCACTCAAGAAGACAGAGAACGCGACCTCAAAACCAAAACCCACATCAAACCTTCTGCCTCTTCCTCTTCCTCTTCCTCATCCATTCGTCATGTTTCCAAGCTCCGAACTGCTTTAACAGTCTTCTCCCGGATCCGACCAACCCACCACCGCTCCCGCCTCGGCGGCCGGGTCATCGGTACACTCTATGGCTACCGACGAGGCCACGTTTATTTCGCATTACAAGAAGACCCAAAACAGAGTCCAACGTTTTTAATCGAGCTCTCGACACCAACCAGCGTTCTGGTTCGAGAAATGGCATCTGGGTTGGTACGGATCGCGTTGGAATGTGAGAAGAAAACAGAGAGGAAGAAGAATTGCAAGTTAGTGGAAGAAGCACTTTGGAGAACGTATTGTAATGGGAAGAAATGTGGGTATGGGAGTAGAAGAGAATGTGGAACAGAGGAGCAGAAGATTTTGAAGGCGGTGGAGCCGATCACGATGGGGGCTGGGGTCTTGCCCCCAGGGCATGGTTCGGATGAGGAATTAATGTATATGAGAGCTAGATTTGAGAGGGTAATTGGGTCGAAGGATTCTGAAGCTTATTACATGATGAGCCCAGATTGTAATGCAGGGCCTGAACTCAGCATTTACTTGCTTAGGGTTTGAACTTTTGATCCCCCCCCCC

Coding sequence (CDS)

ATGTCCAAATCCCTCCATGACTCTTCCTTCTCTTTCTCAAGGCGTTACTTCAACTGGAAGAACAAATTTCACGAAGAAGATGAACAGCAACAACAACACCAACAAATCTCATCTTTCAGTTTATCCTCTCGTTTCACTCAAGAAGACAGAGAACGCGACCTCAAAACCAAAACCCACATCAAACCTTCTGCCTCTTCCTCTTCCTCTTCCTCATCCATTCGTCATGTTTCCAAGCTCCGAACTGCTTTAACAGTCTTCTCCCGGATCCGACCAACCCACCACCGCTCCCGCCTCGGCGGCCGGGTCATCGGTACACTCTATGGCTACCGACGAGGCCACGTTTATTTCGCATTACAAGAAGACCCAAAACAGAGTCCAACGTTTTTAATCGAGCTCTCGACACCAACCAGCGTTCTGGTTCGAGAAATGGCATCTGGGTTGGTACGGATCGCGTTGGAATGTGAGAAGAAAACAGAGAGGAAGAAGAATTGCAAGTTAGTGGAAGAAGCACTTTGGAGAACGTATTGTAATGGGAAGAAATGTGGGTATGGGAGTAGAAGAGAATGTGGAACAGAGGAGCAGAAGATTTTGAAGGCGGTGGAGCCGATCACGATGGGGGCTGGGGTCTTGCCCCCAGGGCATGGTTCGGATGAGGAATTAATGTATATGAGAGCTAGATTTGAGAGGGTAATTGGGTCGAAGGATTCTGAAGCTTATTACATGATGAGCCCAGATTGTAATGCAGGGCCTGAACTCAGCATTTACTTGCTTAGGGTTTGA

Protein sequence

MSKSLHDSSFSFSRRYFNWKNKFHEEDEQQQQHQQISSFSLSSRFTQEDRERDLKTKTHIKPSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFALQEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNGKKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDSEAYYMMSPDCNAGPELSIYLLRV*
Homology
BLAST of CSPI05G24830 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 4.9e-44
Identity = 100/211 (47.39%), Postives = 135/211 (63.98%), Query Frame = 0

Query: 62  PSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFALQED 121
           PS  SSSSS S + +S +            T   S LG RV GTLYG++RGHV F++Q +
Sbjct: 101 PSPPSSSSSVSNQLISLV------------TGGSSSLGRRVTGTLYGHKRGHVTFSVQYN 160

Query: 122 PKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNGKKC 181
            +  P  L++L+  T+ LV+EM+SGLVRIALECEK+   +   KL +E  W  YCNG+KC
Sbjct: 161 QRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEKR--HRSGTKLFQEPKWTMYCNGRKC 220

Query: 182 GYGSRR--ECGTEEQKILKAVEPITMGAGVLPP----------GHGSD-EELMYMRARFE 241
           GY   R   C   + ++L  V  +T+GAGV+P           G G++  EL+YMR +FE
Sbjct: 221 GYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFE 280

Query: 242 RVIGSKDSEAYYMMSPDCNAGPELSIYLLRV 260
           RV+GS+DSEA+YMM+PD N GPELSI+LLR+
Sbjct: 281 RVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

BLAST of CSPI05G24830 vs. ExPASy TrEMBL
Match: A0A0A0KT38 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608220 PE=4 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.8e-137
Identity = 256/261 (98.08%), Postives = 256/261 (98.08%), Query Frame = 0

Query: 1   MSKSLHDSSFSFSRRYFNWKNKFHEEDEQQQQHQQISSFSLSSRFTQEDRERDLKTKTHI 60
           MSKSLHDSSFSFSRRYFNWKNKFHEEDE   QHQQISSFSLSSRFTQEDRERDLKTKTHI
Sbjct: 1   MSKSLHDSSFSFSRRYFNWKNKFHEEDE---QHQQISSFSLSSRFTQEDRERDLKTKTHI 60

Query: 61  KP--SASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFAL 120
           KP  SASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFAL
Sbjct: 61  KPSASASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFAL 120

Query: 121 QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG 180
           QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG
Sbjct: 121 QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG 180

Query: 181 KKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDSEA 240
           KKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDSEA
Sbjct: 181 KKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDSEA 240

Query: 241 YYMMSPDCNAGPELSIYLLRV 260
           YYMMSPDCNAGPELSIYLLRV
Sbjct: 241 YYMMSPDCNAGPELSIYLLRV 258

BLAST of CSPI05G24830 vs. ExPASy TrEMBL
Match: A0A5A7SZ10 (Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00610 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.0e-129
Identity = 245/261 (93.87%), Postives = 249/261 (95.40%), Query Frame = 0

Query: 1   MSKSLHDSSFSFSRRYFNWKNKFHEEDEQQQQH--QQISSFSLSSRFTQEDRERDLKTKT 60
           MSKSLHDSSFSFSRR+FNWKNKFHEEDEQQQQ   QQISSFSLSSRFTQEDRE DL TKT
Sbjct: 1   MSKSLHDSSFSFSRRFFNWKNKFHEEDEQQQQQHDQQISSFSLSSRFTQEDREHDLNTKT 60

Query: 61  HIKPSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFAL 120
           HIKP    SSSSSSIR VSKLRTALTVFSRIRPT+HRSRLGGRVIGTL+GYRRGHVYFAL
Sbjct: 61  HIKP----SSSSSSIRPVSKLRTALTVFSRIRPTYHRSRLGGRVIGTLFGYRRGHVYFAL 120

Query: 121 QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG 180
           QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG
Sbjct: 121 QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG 180

Query: 181 KKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDSEA 240
           KKCGYGSRRE GTEEQKILKAVEPITMGAGVLPPG+GSDEELMYMRARFERVIGSKDSEA
Sbjct: 181 KKCGYGSRREYGTEEQKILKAVEPITMGAGVLPPGNGSDEELMYMRARFERVIGSKDSEA 240

Query: 241 YYMMSPDCNAGPELSIYLLRV 260
           YYMMSPDCN GPELSIYLLRV
Sbjct: 241 YYMMSPDCNGGPELSIYLLRV 257

BLAST of CSPI05G24830 vs. ExPASy TrEMBL
Match: A0A1S3BG36 (protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103489237 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.0e-129
Identity = 245/261 (93.87%), Postives = 249/261 (95.40%), Query Frame = 0

Query: 1   MSKSLHDSSFSFSRRYFNWKNKFHEEDEQQQQH--QQISSFSLSSRFTQEDRERDLKTKT 60
           MSKSLHDSSFSFSRR+FNWKNKFHEEDEQQQQ   QQISSFSLSSRFTQEDRE DL TKT
Sbjct: 1   MSKSLHDSSFSFSRRFFNWKNKFHEEDEQQQQQHDQQISSFSLSSRFTQEDREHDLNTKT 60

Query: 61  HIKPSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFAL 120
           HIKP    SSSSSSIR VSKLRTALTVFSRIRPT+HRSRLGGRVIGTL+GYRRGHVYFAL
Sbjct: 61  HIKP----SSSSSSIRPVSKLRTALTVFSRIRPTYHRSRLGGRVIGTLFGYRRGHVYFAL 120

Query: 121 QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG 180
           QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG
Sbjct: 121 QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG 180

Query: 181 KKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDSEA 240
           KKCGYGSRRE GTEEQKILKAVEPITMGAGVLPPG+GSDEELMYMRARFERVIGSKDSEA
Sbjct: 181 KKCGYGSRREYGTEEQKILKAVEPITMGAGVLPPGNGSDEELMYMRARFERVIGSKDSEA 240

Query: 241 YYMMSPDCNAGPELSIYLLRV 260
           YYMMSPDCN GPELSIYLLRV
Sbjct: 241 YYMMSPDCNGGPELSIYLLRV 257

BLAST of CSPI05G24830 vs. ExPASy TrEMBL
Match: A0A6J1G1C0 (protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449765 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 9.8e-104
Identity = 203/263 (77.19%), Postives = 219/263 (83.27%), Query Frame = 0

Query: 1   MSKSLHDSSFSFSRRYFNWKNKFHEEDEQQQQHQQISSFSLSSRFTQEDRERDLKTKTHI 60
           MS SLHDSS SFSRRYF WKNK  EE    Q HQ+  SF+LSSRF++ED++  LK     
Sbjct: 1   MSNSLHDSSSSFSRRYFTWKNKPQEE----QHHQEPLSFTLSSRFSEEDKQDSLKKPEKK 60

Query: 61  KPSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFALQE 120
           KPSA            SKLRTALTV  RIRPT +RSRLGGRV+GTL+GYRRGHVYFALQE
Sbjct: 61  KPSA------------SKLRTALTVIGRIRPTTNRSRLGGRVVGTLFGYRRGHVYFALQE 120

Query: 121 DPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNGKK 180
           DPKQ+PTFLIELSTPTSVLVREMASGLVRIALECE+K ERKKNCKLVEEALWRTYCNGKK
Sbjct: 121 DPKQNPTFLIELSTPTSVLVREMASGLVRIALECERKAERKKNCKLVEEALWRTYCNGKK 180

Query: 181 CGYGSRRECGTEEQKILKAVEPITMGAGVLP----PGHGSDEELMYMRARFERVIGSKDS 240
           CGY SRRECG EEQ+ILKAVEPITMGAGVLP     G+GS+ ELMYMRARFERVIGSKDS
Sbjct: 181 CGYASRRECGAEEQRILKAVEPITMGAGVLPAAEGSGNGSEGELMYMRARFERVIGSKDS 240

Query: 241 EAYYMMSPDCNAGPELSIYLLRV 260
           EA+YMMSPDCN GPELSIYLLR+
Sbjct: 241 EAFYMMSPDCNGGPELSIYLLRI 247

BLAST of CSPI05G24830 vs. ExPASy TrEMBL
Match: A0A6J1HX62 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467677 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.1e-102
Identity = 202/264 (76.52%), Postives = 221/264 (83.71%), Query Frame = 0

Query: 1   MSKSLHD-SSFSFSRRYFNWKNKFHEEDEQQQQHQQISSFSLSSRFTQEDRERDLKTKTH 60
           MS SLHD SS SFSRRYF WKNK      Q++QHQ+  SF+LSSRF++ED++ +LK    
Sbjct: 1   MSNSLHDSSSSSFSRRYFTWKNK-----PQEEQHQEPLSFTLSSRFSEEDKQDNLKKPEK 60

Query: 61  IKPSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFALQ 120
            KPSA            SKLRTALT   RIRPT +RSRLGGRV+GTL+GYRRGHVYFALQ
Sbjct: 61  KKPSA------------SKLRTALTAIGRIRPTTNRSRLGGRVVGTLFGYRRGHVYFALQ 120

Query: 121 EDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNGK 180
           EDPKQ+PTFLIELSTPTSVLVREMASGLVRIALECE+K ERKKNCKLVEEALWRTYCNGK
Sbjct: 121 EDPKQNPTFLIELSTPTSVLVREMASGLVRIALECERKAERKKNCKLVEEALWRTYCNGK 180

Query: 181 KCGYGSRRECGTEEQKILKAVEPITMGAGVLP----PGHGSDEELMYMRARFERVIGSKD 240
           KCGY SRRECGTEEQ+ILKAVEPITMGAGVLP     G+GS+ ELMYMRARFERVIGSKD
Sbjct: 181 KCGYASRRECGTEEQRILKAVEPITMGAGVLPAAEGSGNGSEGELMYMRARFERVIGSKD 240

Query: 241 SEAYYMMSPDCNAGPELSIYLLRV 260
           SEA+YMMSPDCN GPELSIYLLR+
Sbjct: 241 SEAFYMMSPDCNGGPELSIYLLRI 247

BLAST of CSPI05G24830 vs. NCBI nr
Match: XP_004135413.1 (protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN52034.1 hypothetical protein Csa_008149 [Cucumis sativus])

HSP 1 Score: 498.4 bits (1282), Expect = 3.7e-137
Identity = 256/261 (98.08%), Postives = 256/261 (98.08%), Query Frame = 0

Query: 1   MSKSLHDSSFSFSRRYFNWKNKFHEEDEQQQQHQQISSFSLSSRFTQEDRERDLKTKTHI 60
           MSKSLHDSSFSFSRRYFNWKNKFHEEDE   QHQQISSFSLSSRFTQEDRERDLKTKTHI
Sbjct: 1   MSKSLHDSSFSFSRRYFNWKNKFHEEDE---QHQQISSFSLSSRFTQEDRERDLKTKTHI 60

Query: 61  KP--SASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFAL 120
           KP  SASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFAL
Sbjct: 61  KPSASASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFAL 120

Query: 121 QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG 180
           QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG
Sbjct: 121 QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG 180

Query: 181 KKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDSEA 240
           KKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDSEA
Sbjct: 181 KKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDSEA 240

Query: 241 YYMMSPDCNAGPELSIYLLRV 260
           YYMMSPDCNAGPELSIYLLRV
Sbjct: 241 YYMMSPDCNAGPELSIYLLRV 258

BLAST of CSPI05G24830 vs. NCBI nr
Match: XP_008446535.1 (PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0034517.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa] >TYK09071.1 protein MIZU-KUSSEI 1 [Cucumis melo var. makuwa])

HSP 1 Score: 472.6 bits (1215), Expect = 2.1e-129
Identity = 245/261 (93.87%), Postives = 249/261 (95.40%), Query Frame = 0

Query: 1   MSKSLHDSSFSFSRRYFNWKNKFHEEDEQQQQH--QQISSFSLSSRFTQEDRERDLKTKT 60
           MSKSLHDSSFSFSRR+FNWKNKFHEEDEQQQQ   QQISSFSLSSRFTQEDRE DL TKT
Sbjct: 1   MSKSLHDSSFSFSRRFFNWKNKFHEEDEQQQQQHDQQISSFSLSSRFTQEDREHDLNTKT 60

Query: 61  HIKPSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFAL 120
           HIKP    SSSSSSIR VSKLRTALTVFSRIRPT+HRSRLGGRVIGTL+GYRRGHVYFAL
Sbjct: 61  HIKP----SSSSSSIRPVSKLRTALTVFSRIRPTYHRSRLGGRVIGTLFGYRRGHVYFAL 120

Query: 121 QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG 180
           QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG
Sbjct: 121 QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNG 180

Query: 181 KKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDSEA 240
           KKCGYGSRRE GTEEQKILKAVEPITMGAGVLPPG+GSDEELMYMRARFERVIGSKDSEA
Sbjct: 181 KKCGYGSRREYGTEEQKILKAVEPITMGAGVLPPGNGSDEELMYMRARFERVIGSKDSEA 240

Query: 241 YYMMSPDCNAGPELSIYLLRV 260
           YYMMSPDCN GPELSIYLLRV
Sbjct: 241 YYMMSPDCNGGPELSIYLLRV 257

BLAST of CSPI05G24830 vs. NCBI nr
Match: XP_038892949.1 (protein MIZU-KUSSEI 1 [Benincasa hispida])

HSP 1 Score: 445.7 bits (1145), Expect = 2.8e-121
Identity = 230/263 (87.45%), Postives = 242/263 (92.02%), Query Frame = 0

Query: 1   MSKSLHDSSFSFSRRYFNWKNKFH----EEDEQQQQHQQISSFSLSSRFTQEDRERDLKT 60
           MSKSLHDSSFSFSRRYFNWKNKFH    +++E+QQQHQQI SFSLSSRFT+EDRE DLKT
Sbjct: 1   MSKSLHDSSFSFSRRYFNWKNKFHDSKQQDEEEQQQHQQILSFSLSSRFTEEDRELDLKT 60

Query: 61  KTHIKPSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYF 120
           K   KPS+SS+      R VSKLRTALTVF+RIRPT HRSRLGGRVIGTL+GYRRGHVYF
Sbjct: 61  KIPNKPSSSSN------RPVSKLRTALTVFTRIRPTPHRSRLGGRVIGTLFGYRRGHVYF 120

Query: 121 ALQEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYC 180
           A+QEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTER+KNCKLVEEALWRTYC
Sbjct: 121 AIQEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERRKNCKLVEEALWRTYC 180

Query: 181 NGKKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGSDEELMYMRARFERVIGSKDS 240
           NGKKCGYGSRRE G EEQKILKAVEPITMGAGVLPPG+GSD ELMYMRARFERVIGSKDS
Sbjct: 181 NGKKCGYGSRREYGAEEQKILKAVEPITMGAGVLPPGNGSDGELMYMRARFERVIGSKDS 240

Query: 241 EAYYMMSPDCNAGPELSIYLLRV 260
           EAYYMMSPDCN GPELSIYLLRV
Sbjct: 241 EAYYMMSPDCNGGPELSIYLLRV 257

BLAST of CSPI05G24830 vs. NCBI nr
Match: XP_023541812.1 (protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 386.7 bits (992), Expect = 1.6e-103
Identity = 203/263 (77.19%), Postives = 220/263 (83.65%), Query Frame = 0

Query: 1   MSKSLHDSSFSFSRRYFNWKNKFHEEDEQQQQHQQISSFSLSSRFTQEDRERDLKTKTHI 60
           MS SLHDSS SFSRRYF WKNK  EE    QQHQ+  SF+LSSRF++ED++ +LK     
Sbjct: 92  MSNSLHDSSSSFSRRYFTWKNKPQEE----QQHQEPLSFTLSSRFSEEDKQDNLKKPEKK 151

Query: 61  KPSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFALQE 120
           KPS             SKLRTALTV  RIRPT +RSRLGGRV+GTL+GYRRGHVYFALQE
Sbjct: 152 KPSP------------SKLRTALTVIGRIRPTTNRSRLGGRVVGTLFGYRRGHVYFALQE 211

Query: 121 DPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNGKK 180
           DPKQ+PTFLIELSTPTSVLVREMASGLVRIALECE+K ERKKNCKLVEEALWRTYCNGKK
Sbjct: 212 DPKQNPTFLIELSTPTSVLVREMASGLVRIALECERKAERKKNCKLVEEALWRTYCNGKK 271

Query: 181 CGYGSRRECGTEEQKILKAVEPITMGAGVLP----PGHGSDEELMYMRARFERVIGSKDS 240
           CGY SRRECG EEQ+ILKAVEPITMGAGVLP     G+GS+ ELMYMRARFERVIGSKDS
Sbjct: 272 CGYASRRECGAEEQRILKAVEPITMGAGVLPAAEGSGNGSEGELMYMRARFERVIGSKDS 331

Query: 241 EAYYMMSPDCNAGPELSIYLLRV 260
           EA+YMMSPDCN GPELSIYLLR+
Sbjct: 332 EAFYMMSPDCNGGPELSIYLLRI 338

BLAST of CSPI05G24830 vs. NCBI nr
Match: XP_022945569.1 (protein MIZU-KUSSEI 1-like [Cucurbita moschata])

HSP 1 Score: 386.3 bits (991), Expect = 2.0e-103
Identity = 203/263 (77.19%), Postives = 219/263 (83.27%), Query Frame = 0

Query: 1   MSKSLHDSSFSFSRRYFNWKNKFHEEDEQQQQHQQISSFSLSSRFTQEDRERDLKTKTHI 60
           MS SLHDSS SFSRRYF WKNK  EE    Q HQ+  SF+LSSRF++ED++  LK     
Sbjct: 1   MSNSLHDSSSSFSRRYFTWKNKPQEE----QHHQEPLSFTLSSRFSEEDKQDSLKKPEKK 60

Query: 61  KPSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFALQE 120
           KPSA            SKLRTALTV  RIRPT +RSRLGGRV+GTL+GYRRGHVYFALQE
Sbjct: 61  KPSA------------SKLRTALTVIGRIRPTTNRSRLGGRVVGTLFGYRRGHVYFALQE 120

Query: 121 DPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNGKK 180
           DPKQ+PTFLIELSTPTSVLVREMASGLVRIALECE+K ERKKNCKLVEEALWRTYCNGKK
Sbjct: 121 DPKQNPTFLIELSTPTSVLVREMASGLVRIALECERKAERKKNCKLVEEALWRTYCNGKK 180

Query: 181 CGYGSRRECGTEEQKILKAVEPITMGAGVLP----PGHGSDEELMYMRARFERVIGSKDS 240
           CGY SRRECG EEQ+ILKAVEPITMGAGVLP     G+GS+ ELMYMRARFERVIGSKDS
Sbjct: 181 CGYASRRECGAEEQRILKAVEPITMGAGVLPAAEGSGNGSEGELMYMRARFERVIGSKDS 240

Query: 241 EAYYMMSPDCNAGPELSIYLLRV 260
           EA+YMMSPDCN GPELSIYLLR+
Sbjct: 241 EAFYMMSPDCNGGPELSIYLLRI 247

BLAST of CSPI05G24830 vs. TAIR 10
Match: AT5G23100.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 241.5 bits (615), Expect = 7.5e-64
Identity = 129/247 (52.23%), Postives = 168/247 (68.02%), Query Frame = 0

Query: 52  RDLKTKTHIKPSASSSSSSSS-IRH--------VSKLRTALTVFSRIRPTHHRSRLGGRV 111
           + L    + KPS+SSSSSSS+ I H        VS+LR+ +   SR RP +  S LG RV
Sbjct: 31  KPLPHHNNTKPSSSSSSSSSNVITHKKKLQSFAVSRLRSVIATLSRARPGNQNSGLGSRV 90

Query: 112 IGTLYGYRRGHVYFALQEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKK 171
           +GTL+G RRGHV+F++Q+DP   P FLIEL+TP S LV+EMASGLVRIALEC+K  E ++
Sbjct: 91  VGTLFGSRRGHVHFSIQKDPNSPPAFLIELATPISGLVKEMASGLVRIALECDKGKEEEE 150

Query: 172 ------------------------NCKLVEEALWRTYCNGKKCGYGSRRECGTEEQKILK 231
                                   + +LVEE +WRTYCNGKKCG+ +RRECG +E+K+LK
Sbjct: 151 GEEKNGTLRHGGGDKTKTTTTAAVSRRLVEEPMWRTYCNGKKCGFATRRECGEKEKKVLK 210

Query: 232 AVEPITMGAGVLPP------GHGSDEELMYMRARFERVIGSKDSEAYYMMSPDCNAGPEL 260
           A+E ++MGAGVLP       G G   ++MYMRA+FER++GS+DSEA+YMM+PD N  PEL
Sbjct: 211 ALEMVSMGAGVLPETEEIGGGGGGGGDIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPEL 270

BLAST of CSPI05G24830 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 236.9 bits (603), Expect = 1.8e-62
Identity = 140/269 (52.04%), Postives = 180/269 (66.91%), Query Frame = 0

Query: 7   DSSFSFSRRYFNWKNKFHEEDEQQQQHQQISSFSLSSRFTQEDRERDLKTKTHIK--PSA 66
           DSSFS S+RYFNWK K  +ED+ +++ ++   ++          E+ + T+ +    P+ 
Sbjct: 11  DSSFSLSKRYFNWKKKKVQEDDDEEEEEEEDDYN--------HNEKKVLTRFNFSSDPTR 70

Query: 67  SSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFALQEDPKQ 126
               ++  I    K +T   +  R       S LG RV+GTL+G RRGHVYFA+Q+DP +
Sbjct: 71  PDQFNAQQIMKKKKKKTLEKI--RYALGFSNSGLGFRVVGTLFGNRRGHVYFAVQDDPTR 130

Query: 127 SPTFLIELSTPTSVLVREMASGLVRIALE-CEKKTERKKNCKLVEEALWRTYCNGKKCGY 186
            P  LI+L TPTSVLVREMASGLVRIALE    KT+ KK  KL+EE+ WRTYCNGKKCGY
Sbjct: 131 LPAVLIQLPTPTSVLVREMASGLVRIALETAAYKTDSKK--KLLEESTWRTYCNGKKCGY 190

Query: 187 GSRRECGTEEQKILKAVEPITMGAGVLP--------PGHGS----DEELMYMRARFERVI 246
            +R+ECG  E K+LKAV PITMGAGVLP         G+G+      ELMYMRARFERV+
Sbjct: 191 AARKECGEAEWKVLKAVGPITMGAGVLPATTTTVDEEGNGAVGSEKGELMYMRARFERVV 250

Query: 247 GSKDSEAYYMMSPDCNA-GPELSIYLLRV 260
           GS+DSEA+YMM+PD ++ GPELS+Y LRV
Sbjct: 251 GSRDSEAFYMMNPDVSSGGPELSVYFLRV 267

BLAST of CSPI05G24830 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 179.5 bits (454), Expect = 3.5e-45
Identity = 100/211 (47.39%), Postives = 135/211 (63.98%), Query Frame = 0

Query: 62  PSASSSSSSSSIRHVSKLRTALTVFSRIRPTHHRSRLGGRVIGTLYGYRRGHVYFALQED 121
           PS  SSSSS S + +S +            T   S LG RV GTLYG++RGHV F++Q +
Sbjct: 101 PSPPSSSSSVSNQLISLV------------TGGSSSLGRRVTGTLYGHKRGHVTFSVQYN 160

Query: 122 PKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKTERKKNCKLVEEALWRTYCNGKKC 181
            +  P  L++L+  T+ LV+EM+SGLVRIALECEK+   +   KL +E  W  YCNG+KC
Sbjct: 161 QRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEKR--HRSGTKLFQEPKWTMYCNGRKC 220

Query: 182 GYGSRR--ECGTEEQKILKAVEPITMGAGVLPP----------GHGSD-EELMYMRARFE 241
           GY   R   C   + ++L  V  +T+GAGV+P           G G++  EL+YMR +FE
Sbjct: 221 GYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTIDDVSGVGSGTELGELLYMRGKFE 280

Query: 242 RVIGSKDSEAYYMMSPDCNAGPELSIYLLRV 260
           RV+GS+DSEA+YMM+PD N GPELSI+LLR+
Sbjct: 281 RVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

BLAST of CSPI05G24830 vs. TAIR 10
Match: AT2G21990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 177.6 bits (449), Expect = 1.3e-44
Identity = 86/173 (49.71%), Postives = 123/173 (71.10%), Query Frame = 0

Query: 91  PTHHRS-RLGGRVIGTLYGYRRGHVYFALQEDPKQSPTFLIELSTPTSVLVREMASGLVR 150
           P  HRS   G RV GTL+GYR+G V  ++QE P+  P+ ++EL+  T VL +E++ G+VR
Sbjct: 81  PDQHRSGSSGSRVTGTLFGYRKGRVSLSIQESPRCLPSLVVELAMQTMVLQKELSGGMVR 140

Query: 151 IALECEKKTERKKNCKLVEEALWRTYCNGKKCGYGSRRECGTEEQKILKAVEPITMGAGV 210
           IALE EK+ +++K  K+++E LW  + NGKK GYG +R+   E+  +++ + P++MGAGV
Sbjct: 141 IALETEKRGDKEK-IKIMDEPLWTMFSNGKKTGYGVKRDATEEDLNVMELLRPVSMGAGV 200

Query: 211 LPPG---HGSDEELMYMRARFERVIGSKDSEAYYMMSPDCNAGPELSIYLLRV 260
           LP      G D E+ YMRA FERV+GSKDSE +YM+SP+ N GPELSI+ +RV
Sbjct: 201 LPGNTEFEGPDSEMAYMRAYFERVVGSKDSETFYMLSPEGNNGPELSIFFVRV 252

BLAST of CSPI05G24830 vs. TAIR 10
Match: AT5G06990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 175.3 bits (443), Expect = 6.6e-44
Identity = 86/174 (49.43%), Postives = 118/174 (67.82%), Query Frame = 0

Query: 99  GGRVIGTLYGYRRGHVYFALQEDPKQSPTFLIELSTPTSVLVREMASGLVRIALECEKKT 158
           G RV GTL+GYR+  V  A+QE+P+  P  L+EL+ PT  L++++  GLVRIALECEKK 
Sbjct: 90  GTRVTGTLFGYRKTRVNLAVQENPRSLPILLLELAIPTGKLLQDLGVGLVRIALECEKKP 149

Query: 159 ERKKNCKLVEEALWRTYCNGKKCGYGSRRECGTEEQKILKAVEPITMGAGVLPPGHGS-- 218
             K   K+++E +W  YCNGKK GYG +R+   E+  +++ +  ++MGAGVLP   G+  
Sbjct: 150 SEK--TKIIDEPIWALYCNGKKSGYGVKRQPTEEDLVVMQMLHAVSMGAGVLPVSSGAIT 209

Query: 219 -----------DEELMYMRARFERVIGSKDSEAYYMMSPDCNAGPELSIYLLRV 260
                      + +L YMRA FERVIGS+DSE YYMM+PD N+GPELSI+ +RV
Sbjct: 210 EQSGGGGGGQQEGDLTYMRAHFERVIGSRDSETYYMMNPDGNSGPELSIFFVRV 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O222274.9e-4447.39Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KT381.8e-13798.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608220 PE=4 SV=1[more]
A0A5A7SZ101.0e-12993.87Protein MIZU-KUSSEI 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold47... [more]
A0A1S3BG361.0e-12993.87protein MIZU-KUSSEI 1 OS=Cucumis melo OX=3656 GN=LOC103489237 PE=4 SV=1[more]
A0A6J1G1C09.8e-10477.19protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449765 PE=4 SV... [more]
A0A6J1HX621.1e-10276.52protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467677 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004135413.13.7e-13798.08protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN52034.1 hypothetical protein Csa_008... [more]
XP_008446535.12.1e-12993.87PREDICTED: protein MIZU-KUSSEI 1 [Cucumis melo] >KAA0034517.1 protein MIZU-KUSSE... [more]
XP_038892949.12.8e-12187.45protein MIZU-KUSSEI 1 [Benincasa hispida][more]
XP_023541812.11.6e-10377.19protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo][more]
XP_022945569.12.0e-10377.19protein MIZU-KUSSEI 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G23100.17.5e-6452.23Protein of unknown function, DUF617 [more]
AT3G25640.11.8e-6252.04Protein of unknown function, DUF617 [more]
AT2G41660.13.5e-4547.39Protein of unknown function, DUF617 [more]
AT2G21990.11.3e-4449.71Protein of unknown function, DUF617 [more]
AT5G06990.16.6e-4449.43Protein of unknown function, DUF617 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006460Protein MIZU-KUSSEI 1-like, plantTIGRFAMTIGR01570TIGR01570coord: 102..259
e-value: 3.7E-67
score: 223.3
IPR006460Protein MIZU-KUSSEI 1-like, plantPFAMPF04759DUF617coord: 102..258
e-value: 1.6E-67
score: 226.6
IPR006460Protein MIZU-KUSSEI 1-like, plantPANTHERPTHR31696PROTEIN MIZU-KUSSEI 1coord: 1..259
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..75
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 60..75
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..59
NoneNo IPR availablePANTHERPTHR31696:SF14OS01G0642600 PROTEINcoord: 1..259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G24830.1CSPI05G24830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010274 hydrotropism