CSPI05G24760 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G24760
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Calmodulin, putative
Location	Chr5: 24265368 .. 24266361 (+)
RNA-Seq Expression	CSPI05G24760
Synteny	CSPI05G24760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGAAGTCAAGAAAAGAAAAGAGGGTAATGAGCTGTGTGTTGTGTTGATCCATTTTGGATCGAAATTCCCAAAGAACGCGGTTTGTTAATTGAAGTTTCAAACAGTTTCCCTTTCAAACCTCACATCCCTAACTGACCAATTTCCTTCCTTTTCCCCATTTTTTCCAAAACCCCTTCTAACCTCCTCCATCGGATCTTGTCGAATCGTCTGAAACCATTTCCATGGCTTCCATTCAATCCGACCACCAGCTCAAGCAGCTGCACGACATTTTCAGGCGTTTCGACATGAATTCCGATGGCAGTCTCACCCAACTCGAACTCGGCGCCCTCCTCCGCTCCCTCGGCATCAAACCCTCCGGCGACCAACTCCATTCTCTCCTCTCCAACATGGATTCCAACGGCAACGGCTCCATCGAATTCGATGAACTCGTCAATGCCATTCTCCCCGATATGAACGATGATATTCTCGTCAACCAAGAGCAGTTGATGGAGGTTTTCAGATCTTTCGATCGCGATGGTAATGGCTACATCACCGCCGCCGAGCTCGCTGGTTCCATGGCCAAAATGGGCCATCCTCTCACCTACCGGGAACTCTCCGATATGATGCGCCAGGCCGATACCGACGGCGACGGCGTTATTAGCTTCAATGAATTCACCACCGTCATGGCTAAATCCGCCGCGGATTTTCTCGGGCTTACATTTGCGTAGATCAAGTTCTTCATTCCCTCCCCCTCTTTTTCTTTAGATGTACATAGCAAGATATTATAACCATTCTGATCGTGATCCTGCTCCTGTTCCTGATTCTGATTCTAATCGGTGTTCATCTTCGGTGATTAATTTCTCCATAAGTTTTTCTGGGTAAATCACCAAATTCCAAATAGAGAAAGAGAGAATCAATCAAATGTTCAAAACTATGGTTTGGTCCAATGATGTGTTTCAACTCTGTATTCTAAATTCCAACTTGCTTCATCAACTTGTATTATTTCTTCCCCTA

mRNA sequence

GAGAAGTCAAGAAAAGAAAAGAGGGTAATGAGCTGTGTGTTGTGTTGATCCATTTTGGATCGAAATTCCCAAAGAACGCGGTTTGTTAATTGAAGTTTCAAACAGTTTCCCTTTCAAACCTCACATCCCTAACTGACCAATTTCCTTCCTTTTCCCCATTTTTTCCAAAACCCCTTCTAACCTCCTCCATCGGATCTTGTCGAATCGTCTGAAACCATTTCCATGGCTTCCATTCAATCCGACCACCAGCTCAAGCAGCTGCACGACATTTTCAGGCGTTTCGACATGAATTCCGATGGCAGTCTCACCCAACTCGAACTCGGCGCCCTCCTCCGCTCCCTCGGCATCAAACCCTCCGGCGACCAACTCCATTCTCTCCTCTCCAACATGGATTCCAACGGCAACGGCTCCATCGAATTCGATGAACTCGTCAATGCCATTCTCCCCGATATGAACGATGATATTCTCGTCAACCAAGAGCAGTTGATGGAGGTTTTCAGATCTTTCGATCGCGATGGTAATGGCTACATCACCGCCGCCGAGCTCGCTGGTTCCATGGCCAAAATGGGCCATCCTCTCACCTACCGGGAACTCTCCGATATGATGCGCCAGGCCGATACCGACGGCGACGGCGTTATTAGCTTCAATGAATTCACCACCGTCATGGCTAAATCCGCCGCGGATTTTCTCGGGCTTACATTTGCGTAGATCAAGTTCTTCATTCCCTCCCCCTCTTTTTCTTTAGATGTACATAGCAAGATATTATAACCATTCTGATCGTGATCCTGCTCCTGTTCCTGATTCTGATTCTAATCGGTGTTCATCTTCGGTGATTAATTTCTCCATAAGTTTTTCTGGGTAAATCACCAAATTCCAAATAGAGAAAGAGAGAATCAATCAAATGTTCAAAACTATGGTTTGGTCCAATGATGTGTTTCAACTCTGTATTCTAAATTCCAACTTGCTTCATCAACTTGTATTATTTCTTCCCCTA

Coding sequence (CDS)

ATGGCTTCCATTCAATCCGACCACCAGCTCAAGCAGCTGCACGACATTTTCAGGCGTTTCGACATGAATTCCGATGGCAGTCTCACCCAACTCGAACTCGGCGCCCTCCTCCGCTCCCTCGGCATCAAACCCTCCGGCGACCAACTCCATTCTCTCCTCTCCAACATGGATTCCAACGGCAACGGCTCCATCGAATTCGATGAACTCGTCAATGCCATTCTCCCCGATATGAACGATGATATTCTCGTCAACCAAGAGCAGTTGATGGAGGTTTTCAGATCTTTCGATCGCGATGGTAATGGCTACATCACCGCCGCCGAGCTCGCTGGTTCCATGGCCAAAATGGGCCATCCTCTCACCTACCGGGAACTCTCCGATATGATGCGCCAGGCCGATACCGACGGCGACGGCGTTATTAGCTTCAATGAATTCACCACCGTCATGGCTAAATCCGCCGCGGATTTTCTCGGGCTTACATTTGCGTAG

Protein sequence

MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA*

Homology

[Close]

BLAST of CSPI05G24760 vs. ExPASy Swiss-Prot
Match: Q9LI84 (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 PE=2 SV=2)

HSP 1 Score: 246.9 bits (629), Expect = 1.6e-64
Identity = 123/159 (77.36%), Postives = 142/159 (89.31%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS +   Q+KQL DIF RFDM+ DGSLTQLEL ALLRSLGIKP GDQ+  LL+ +D NG
Sbjct: 1   MASTKPTDQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGS+EFDELV AILPD+N+++L+NQEQLMEVFRSFDRDGNG ITAAELAGSMAKMGHPLT
Sbjct: 61  NGSVEFDELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLT 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLT 160
           YREL++MM +AD++GDGVISFNEF+ +MAKSAADFLGLT
Sbjct: 121 YRELTEMMTEADSNGDGVISFNEFSHIMAKSAADFLGLT 159

[Close]

BLAST of CSPI05G24760 vs. ExPASy Swiss-Prot
Match: Q9FZ75 (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 4.4e-59
Identity = 106/152 (69.74%), Postives = 137/152 (90.13%), Query Frame = 0


Query: 7   DHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 66
           + Q++QL DIF RFDM++DGSLT LEL ALLRSLG+KPSGDQ+H LL++MDSNGNG +EF
Sbjct: 2   EDQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEF 61

Query: 67  DELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELSD 126
           DELV  ILPD+N+++L+N EQL+E+F+SFDRDGNG+I+AAELAG+MAKMG PLTY+EL++
Sbjct: 62  DELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTE 121

Query: 127 MMRQADTDGDGVISFNEFTTVMAKSAADFLGL 159
           M+++ADT+GDGVISF EF ++MAKSA D+ GL
Sbjct: 122 MIKEADTNGDGVISFGEFASIMAKSAVDYFGL 153

[Close]

BLAST of CSPI05G24760 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.5e-54
Identity = 106/151 (70.20%), Postives = 128/151 (84.77%), Query Frame = 0


Query: 9   QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEFDE 68
           QLKQL ++FRRFDMN DGSLTQLEL ALLRSLG++P+GD++H+LL+ MD+NGNGS+EFDE
Sbjct: 22  QLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGSVEFDE 81

Query: 69  LVNAILPDMNDDI-LVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELSDM 128
           L  AI P +     LV+Q QL+EVFR+FDRDGNG+I+AAELA SMA++G PLT+ EL+ M
Sbjct: 82  LAAAIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFEELTRM 141

Query: 129 MRQADTDGDGVISFNEFTTVMAKSAADFLGL 159
           MR ADTDGDGVISF EF  VMAKSA DFLG+
Sbjct: 142 MRDADTDGDGVISFKEFAAVMAKSALDFLGV 172

[Close]

BLAST of CSPI05G24760 vs. ExPASy Swiss-Prot
Match: Q5ZD81 (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 GN=CML12 PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 6.4e-50
Identity = 97/155 (62.58%), Postives = 129/155 (83.23%), Query Frame = 0


Query: 9   QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKP-SGDQLHSLLSNMDSNGNGSIEFD 68
           QL+QLH+IF RFD++ DGSLT+LEL ALLRSLG++P +GD++H+L++ +D++GNG++EFD
Sbjct: 92  QLRQLHEIFLRFDLDGDGSLTKLELAALLRSLGLRPAAGDEIHALIAAIDADGNGTVEFD 151

Query: 69  ELVNA----ILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRE 128
           EL ++    IL      + V+Q +L E FR+FDRDGNG+I+AAELA SMA+MGHP+ Y E
Sbjct: 152 ELASSLADLILGPCRPSVAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGHPICYAE 211

Query: 129 LSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGL 159
           L+DMMR+ADTDGDG+ISF EFT +MAKSA DFLGL
Sbjct: 212 LTDMMREADTDGDGLISFEEFTAIMAKSALDFLGL 246

[Close]

BLAST of CSPI05G24760 vs. ExPASy Swiss-Prot
Match: Q9AWK2 (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 GN=CML11 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.6e-40
Identity = 85/149 (57.05%), Postives = 110/149 (73.83%), Query Frame = 0


Query: 7   DHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 66
           D QL +L +IFR FD N DGSLTQLELG+LLRSLG+KPS D+L SL+   D+N NG IEF
Sbjct: 59  DDQLGELREIFRSFDRNGDGSLTQLELGSLLRSLGLKPSTDELDSLIQRADTNSNGLIEF 118

Query: 67  DELVNAILPD-MNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELS 126
            E V  + P+ + D    +++Q+  +F  FDRDGNG+ITAAELA SMAK+GH LT +EL+
Sbjct: 119 SEFVALVAPELLYDRAPYSEDQIRRLFNIFDRDGNGFITAAELAHSMAKLGHALTVKELT 178

Query: 127 DMMRQADTDGDGVISFNEFTTVMAKSAAD 155
            M+++ADTDGDG ISF EF+  +  +A D
Sbjct: 179 GMIKEADTDGDGRISFQEFSRAITAAAFD 207

[Close]

BLAST of CSPI05G24760 vs. ExPASy TrEMBL
Match: A0A0A0KUE0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608150 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 5.9e-83
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT
Sbjct: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 161

[Close]

BLAST of CSPI05G24760 vs. ExPASy TrEMBL
Match: A0A1S3BF88 (probable calcium-binding protein CML16 OS=Cucumis melo OX=3656 GN=LOC103489229 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.7e-82
Identity = 160/161 (99.38%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT
Sbjct: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELS+MMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 161

[Close]

BLAST of CSPI05G24760 vs. ExPASy TrEMBL
Match: A0A6J1JA36 (probable calcium-binding protein CML16 OS=Cucurbita maxima OX=3661 GN=LOC111484890 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.9e-77
Identity = 151/161 (93.79%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS+QSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASMQSD-QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPD+ND+ILVNQEQLMEVF+SFDRDGNG+ITA+ELAGSMAKMGHPL+
Sbjct: 61  NGSIEFDELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLS 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELS+MMRQADTDGDGVISFNEFTTVMA+SAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMARSAADFLGLTFA 160

[Close]

BLAST of CSPI05G24760 vs. ExPASy TrEMBL
Match: A0A6J1GX12 (probable calcium-binding protein CML16 OS=Cucurbita moschata OX=3662 GN=LOC111458327 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.1e-76
Identity = 150/161 (93.17%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS+QSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASLQSD-QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEF+ELVNAILPD+ND+ILVNQEQLMEVF+SFDRDGNG+ITA+ELAGSMAKMGHPL+
Sbjct: 61  NGSIEFEELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLS 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELS+MMRQADTDGDGVISFNEFTTVMA+SAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMARSAADFLGLTFA 160

[Close]

BLAST of CSPI05G24760 vs. ExPASy TrEMBL
Match: A0A6J1D725 (probable calcium-binding protein CML16 OS=Momordica charantia OX=3673 GN=LOC111017528 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.7e-75
Identity = 151/161 (93.79%), Postives = 156/161 (96.89%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MASIQSD QLKQL DIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASIQSD-QLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEF+ELVNAILPDMNDDILVNQEQL+EVFRSFDRDGNGYITA+ELAGSMAKMG PL+
Sbjct: 61  NGSIEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLS 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELS+MMR AD DGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YRELSEMMRLADIDGDGVISFNEFTTVMAKSAADFLGLTFA 160

[Close]

BLAST of CSPI05G24760 vs. NCBI nr
Match: XP_004135127.1 (probable calcium-binding protein CML16 [Cucumis sativus] >KGN52027.1 hypothetical protein Csa_008611 [Cucumis sativus])

HSP 1 Score: 316.6 bits (810), Expect = 1.2e-82
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT
Sbjct: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 161

[Close]

BLAST of CSPI05G24760 vs. NCBI nr
Match: XP_008446524.1 (PREDICTED: probable calcium-binding protein CML16 [Cucumis melo])

HSP 1 Score: 315.1 bits (806), Expect = 3.6e-82
Identity = 160/161 (99.38%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT
Sbjct: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELS+MMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 161

[Close]

BLAST of CSPI05G24760 vs. NCBI nr
Match: XP_038891550.1 (probable calcium-binding protein CML16 [Benincasa hispida])

HSP 1 Score: 305.8 bits (782), Expect = 2.2e-79
Identity = 154/161 (95.65%), Postives = 159/161 (98.76%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS+QSD Q+KQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASLQSDQQVKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPDMNDDILVNQEQL++VFRSFDRDGNGYITAAELAGSMAKMGHPLT
Sbjct: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLDVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           Y ELS+MMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YHELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 161

[Close]

BLAST of CSPI05G24760 vs. NCBI nr
Match: KAG6601337.1 (putative calcium-binding protein CML16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 297.4 bits (760), Expect = 7.7e-77
Identity = 151/161 (93.79%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS+QSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASLQSD-QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPD+ND+ILVNQEQLMEVF+SFDRDGNG+ITA+ELAGSMAKMGHPL+
Sbjct: 61  NGSIEFDELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLS 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELS+MMRQADTDGDGVISFNEFTTVMA+SAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMARSAADFLGLTFA 160

[Close]

BLAST of CSPI05G24760 vs. NCBI nr
Match: XP_022987337.1 (probable calcium-binding protein CML16 [Cucurbita maxima])

HSP 1 Score: 297.0 bits (759), Expect = 1.0e-76
Identity = 151/161 (93.79%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS+QSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASMQSD-QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPD+ND+ILVNQEQLMEVF+SFDRDGNG+ITA+ELAGSMAKMGHPL+
Sbjct: 61  NGSIEFDELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLS 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELS+MMRQADTDGDGVISFNEFTTVMA+SAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMARSAADFLGLTFA 160

[Close]

BLAST of CSPI05G24760 vs. TAIR 10
Match: AT3G25600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 246.9 bits (629), Expect = 1.1e-65
Identity = 123/159 (77.36%), Postives = 142/159 (89.31%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS +   Q+KQL DIF RFDM+ DGSLTQLEL ALLRSLGIKP GDQ+  LL+ +D NG
Sbjct: 1   MASTKPTDQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGS+EFDELV AILPD+N+++L+NQEQLMEVFRSFDRDGNG ITAAELAGSMAKMGHPLT
Sbjct: 61  NGSVEFDELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLT 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLT 160
           YREL++MM +AD++GDGVISFNEF+ +MAKSAADFLGLT
Sbjct: 121 YRELTEMMTEADSNGDGVISFNEFSHIMAKSAADFLGLT 159

[Close]

BLAST of CSPI05G24760 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 228.8 bits (582), Expect = 3.1e-60
Identity = 106/152 (69.74%), Postives = 137/152 (90.13%), Query Frame = 0


Query: 7   DHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 66
           + Q++QL DIF RFDM++DGSLT LEL ALLRSLG+KPSGDQ+H LL++MDSNGNG +EF
Sbjct: 2   EDQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEF 61

Query: 67  DELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELSD 126
           DELV  ILPD+N+++L+N EQL+E+F+SFDRDGNG+I+AAELAG+MAKMG PLTY+EL++
Sbjct: 62  DELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTE 121

Query: 127 MMRQADTDGDGVISFNEFTTVMAKSAADFLGL 159
           M+++ADT+GDGVISF EF ++MAKSA D+ GL
Sbjct: 122 MIKEADTNGDGVISFGEFASIMAKSAVDYFGL 153

[Close]

BLAST of CSPI05G24760 vs. TAIR 10
Match: AT3G03000.1 (EF hand calcium-binding protein family )

HSP 1 Score: 164.1 bits (414), Expect = 9.5e-41
Identity = 84/148 (56.76%), Postives = 103/148 (69.59%), Query Frame = 0


Query: 7   DHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 66
           D QL +L +IFR FD N DGSLT+LELG+LLRSLG+KPS DQL +L+   D N NG +EF
Sbjct: 15  DEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEF 74

Query: 67  DELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELSD 126
            E V  + PD+        +QL  +FR FDRDGNGYITAAELA SMAK+GH LT  EL+ 
Sbjct: 75  SEFVALVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTG 134

Query: 127 MMRQADTDGDGVISFNEFTTVMAKSAAD 155
           M+++AD DGDG I F EF   +  +A D
Sbjct: 135 MIKEADRDGDGCIDFQEFVQAITSAAFD 161

[Close]

BLAST of CSPI05G24760 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 155.2 bits (391), Expect = 4.4e-38
Identity = 78/152 (51.32%), Postives = 105/152 (69.08%), Query Frame = 0


Query: 7   DHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 66
           + Q+ +L +IFR FD N DGSLTQLELG+LLR+LG+KPS DQ  +L+   D+  NG +EF
Sbjct: 11  EEQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADTKSNGLVEF 70

Query: 67  DELVNAILPDM----NDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYR 126
            E V  + P++           +EQL+ +FR FD DGNG+ITAAELA SMAK+GH LT  
Sbjct: 71  PEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAKLGHALTVA 130

Query: 127 ELSDMMRQADTDGDGVISFNEFTTVMAKSAAD 155
           EL+ M+++AD+DGDG I+F EF   +  +A D
Sbjct: 131 ELTGMIKEADSDGDGRINFQEFAKAINSAAFD 162

[Close]

BLAST of CSPI05G24760 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 124.4 bits (311), Expect = 8.3e-29
Identity = 61/148 (41.22%), Postives = 95/148 (64.19%), Query Frame = 0


Query: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MA   +D Q+ +  + F  FD + DG +T  ELG ++RSLG  P+  +L  +++ +D++G
Sbjct: 1   MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NG+I+F E +N +   M D    ++E+L E FR FD+D NG+I+AAEL   M  +G  LT
Sbjct: 61  NGTIDFPEFLNLMAKKMKD--TDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLT 120

Query: 121 YRELSDMMRQADTDGDGVISFNEFTTVM 149
             E+ +M+R+AD DGDG I++ EF  +M
Sbjct: 121 DEEVEEMIREADVDGDGQINYEEFVKIM 146

The following BLAST results are available for this feature:

BLAST of CSPI05G24760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LI84	1.6e-64	77.36	Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 ...	[more]
Q9FZ75	4.4e-59	69.74	Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 ...	[more]
Q6L5F4	2.5e-54	70.20	Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q5ZD81	6.4e-50	62.58	Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9AWK2	1.6e-40	57.05	Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CSPI05G24760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KUE0	5.9e-83	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608150 PE=4 SV=1	[more]
A0A1S3BF88	1.7e-82	99.38	probable calcium-binding protein CML16 OS=Cucumis melo OX=3656 GN=LOC103489229 P...	[more]
A0A6J1JA36	4.9e-77	93.79	probable calcium-binding protein CML16 OS=Cucurbita maxima OX=3661 GN=LOC1114848...	[more]
A0A6J1GX12	1.1e-76	93.17	probable calcium-binding protein CML16 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1D725	2.7e-75	93.79	probable calcium-binding protein CML16 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of CSPI05G24760 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135127.1	1.2e-82	100.00	probable calcium-binding protein CML16 [Cucumis sativus] >KGN52027.1 hypothetica...	[more]
XP_008446524.1	3.6e-82	99.38	PREDICTED: probable calcium-binding protein CML16 [Cucumis melo]	[more]
XP_038891550.1	2.2e-79	95.65	probable calcium-binding protein CML16 [Benincasa hispida]	[more]
KAG6601337.1	7.7e-77	93.79	putative calcium-binding protein CML16, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022987337.1	1.0e-76	93.79	probable calcium-binding protein CML16 [Cucurbita maxima]	[more]

BLAST of CSPI05G24760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25600.1	1.1e-65	77.36	Calcium-binding EF-hand family protein	[more]
AT1G18530.1	3.1e-60	69.74	EF hand calcium-binding protein family	[more]
AT3G03000.1	9.5e-41	56.76	EF hand calcium-binding protein family	[more]
AT1G32250.1	4.4e-38	51.32	Calcium-binding EF-hand family protein	[more]
AT1G66410.1	8.3e-29	41.22	calmodulin 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 87..115 e-value: 2.7E-4 score: 30.3 coord: 123..151 e-value: 3.1E-6 score: 36.7 coord: 12..40 e-value: 0.051 score: 22.7 coord: 48..76 e-value: 1.8 score: 15.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 11..72 e-value: 2.9E-12 score: 46.9 coord: 86..148 e-value: 7.5E-15 score: 55.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 44..79 score: 10.356991
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 8..43 score: 12.811786
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 119..154 score: 13.286007
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 83..118 score: 14.987627
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 87..149 e-value: 1.20863E-17 score: 70.6545
None	No IPR available	GENE3D	1.10.238.10		coord: 82..156 e-value: 2.3E-23 score: 84.2
None	No IPR available	GENE3D	1.10.238.10		coord: 1..81 e-value: 9.5E-19 score: 69.5
None	No IPR available	PANTHER	PTHR23050:SF351	CALCIUM-BINDING PROTEIN CML15-RELATED	coord: 7..159
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 7..159
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 21..33
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 57..69
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 132..144
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 96..108
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G24760.1	CSPI05G24760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity