CSPI05G24610 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G24610
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionchaperonin 60 beta
LocationChr5: 24170194 .. 24174840 (-)
RNA-Seq ExpressionCSPI05G24610
SyntenyCSPI05G24610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACTCCCATTCGCCGGCATATCTTCCTCTTATCTTCATCCTTCACTGCGAGTACCAGAACTATGGCGTCTTCTCCCACTCCCATTTCTCCACTATCTTTTACCAATCCAATATCAACATCAAGAACGAAACCAAAACCATTACCATTTCCAAACAAACCGCCGTCTTCATTGCCTCCTGCGTTGAACTCCACCCCCAAACCTCCGCCCAAGGACCTTTACTTCAACCACGATGGCTCTACTTTTAAAAAACTTCAGGTTACGTACTCGGACTAAGCACTTGTTGTTTCTTTGCCCCAAGGGTTTTGCTTTTGTTCGATGATTGAACAGGTTGATTTTGGTGAACAGGTCGGAGTTAATTTGGTAGCGGAGCTGGTGGGTATTACCTTGGGACCGAAGGGGAGGAATGTGGTGCTTCAGAATAAATATGGACCGCCTAAGATCGTCAACGATGGTGAAACCGTCCTGAAAGAGGTATTCTATTTTTCCCCCCTCTTTTTTATGAATCTTTTGGTTAATGTCTTTCTCTTATGCGGTGAAGAATCACAATGAGATTTTTTTTCTTGATATCTATGACTGTATGGGCCATTGGCCAGCTTGGGGATTTCTTTTAAACAGTTCAGTAAAGGTGTATAGTACCTTGACAACTTAATGTAAATTGAAATTGTTTTTGATGATAAGTTTATGTATAATATAACTTTGGGATCAGTGATAAAAGGGAAAGAAATTCGTTTGAAAATCCCCTTGCAAAATTCAGCATGTCTAGGCTTATGAATGATGACACAAGTACTTATTCAAGGTCATCTGTAAATTCTCTTGTGTATCCTCTAAACTAAGATTATTCTTTTGGAATTTGATTATGATCTGGTTGGAAAGCTAGATTGAGCTGGAAGACCCTTTAGAGAACGTTGGGGTGAAATTGGTGAGACAAGCTGGTGCTAAGACGAATGACCTTGCTGGTGATGGTTCCACTACATCTGTCGTTCTTGCCCAAGGATTGATTGCTGAGGGTATGAAGGTAGTTAATGGAATTCCATGCATGAATATCAGTCGTCTAAAACAACTAGTACTGCTATTTGGACAATGGAATACCGTACCCGATTTTAACATTTAACGAAGTTTTCAATAACTTGTAATTCATTATTAAAAAGACGAATGAACGTACCATGTAAAAAACAAAACAAATTTTGTATATTATAAATAATTAAATTTTAAATTTCATATTGATTATTATTTTTTCTATATTTAATTTATTGGTACACGATTTATGATTTAAATATCTAAAATATTCAAGTTACACATACACACCCCCACAATGTAAATTTTCTATAGTGGTGCTGTTATTGGACTCAGTCTAATGACTTGGGCAAATAGCCTAATAACTGGGCTGATTAATGGCCAAACTAGTTAATCTTTGACTCTTGGTTTGGATTAACTTTGAGATTTCCTAGGACTATTGGTGTTTAGATATTGGGTTGTTTGATGAAACTAGCTTTCAGTCTCTATCATATGCAGCGATGAGGTTTTCTGTACTATGAAAGAACAATAAATTATTTTAACTATTTTTCTCACCACAATTAATAGTCTGGCCAGTTTTTAAGCACTAATAATAAAATGCACTTTGTAGGTTATTGCATCTGGCATGAATCCTGTTCAAATTGCACGTGGGATTGAGAAGACTGCAAAAGCACTAGTTTCTGAGCTGAAATTGATGTCAAGAGAGGTAAGTAAGGTTATGCTTCTTTCAGTGTGGAAGGTTCAAAACCTTGAGACGTGCTTCACAGTGAATGAAAAGACAAAGTATCAATTTTAGCCGCAAGCGGTAGTCTACTTACAGTTTGTCAGTGATCTATTCAACTGCTACATATTTCAAACTTGTAGGTTGAAGACCACGAGATAGCACACGTGGCCGCAGTTAGCGCAGGGAATGATTATGCTGTGGGAAGTTTGGTTTCAGATGCCTTACGTCAAGTTGGATGGAAGGGAGTCGTCCAAATTGAAAAAGGGAAGAGTGTGGATAATAGTTTACAAATTGTTGAAGGGATGCAATTTGATCGTGGATATTTATCCCCATACTTCGTGACTGATAGAAGGAAGATGGTAGTAGAATTCCATGAGTGCAAGGTAGCAACCGGACTTTAAATAATATGTTTTTCTTTTATATAAAAACGTAATCTTTAGAAATGGGGATTTTTGGTTGCAGTTACTGTTGGTCGACAAAAAAATTTCCGATCCAAAGGAGATGTTTAAAATATTGGACAATGCAGTGAAAGAGAAATACCCAATTGTGATATTGGCAGAGGGCATTGAGCCGGAAGCTCTGGCTCCAGTAATTAGGAATAAACTTAGAGGTGTGCTGAAGGCAGCCGCTATCAAGGCTCCTGCCTTTGGCGAGCGCAAGAGTCACTATTTAGATGACATAGCCACTTTAACTGGAGGTAGCAGATTATTAGAAGAGAGTTTTGAGTCCTTGTGCTTTCCCCATTTTTCTTAACTCTAAAACCATCTGCAAGTCAAAAAAGAAAGAAATCCAAACCCCCCCCAAAAAAGATGAAAAATAAGGAAAAGGGCACTGTTTAAATGCAACTAATTTATAGATACATGGAGTGGTGCAATACTACATTCAATGAGATTTCAACATAAACGTATATCAGAGATAAAGGGAATCAGTCAAAGACATTTCATGATGATAATAGGATGAAATACCATGGATTTTCAAGGATGATGCATTTAATTAAGTATAACATCATAGTCATCATTTAACTAAATACTATCATCTTTCATTAAAAAAAAAAAAACTATCATCTTGATGCAATGGAGCAAAGCTTATCTTTGAGCTTATTTACATGATGTTAATACAAAGAAAATGCATTTTACCTGTAGTTTTCTGTTTGTTCTTGGAAGATTTTGATCTTAAATTTGATCCGAAGTGCAACTCTTTGATATGTTTGCAGCTACCGTGGTCAGAGAAGATAATGGATTGACGTTAGAAAAGACAGGCAAGGAGGTATTGGGCTCCGCTTGTAAGGTTGTTATATCAAAAGATTCCACACTGATAGTTACAGATGGGAATACTCGGGAAGCTGTTCAGAAAAGGGTCTTACAAATTCAAAAGCTTATGGAGGTGCTGGAATCATCCCTTATCTAATGCTGAGATATCCTACATTTCGAGGTTTTCTGCATTGCAGGATCCATTTCTGAGTAATATAAAAGAACATAAATTTCCGTGATGAAGTATATTGCTTTGACGTTTAGTTGAATACACCAAGTGACATACTATTATCTATTCTGCAGAACACTGAGGAAAAATTTCCGAAGAAGATATTGAATGAGAGAATTGCAAGATTATCTGGGCGAATTGCAATAATTCAGGTCAAATGATTTACAATTATCATCATCCGGAATTAGCAATTTACACCAGACTCATTCACTTTGTCCATATTCTAAATTGTAGGTAGGAGCACAAACTGAAGTTGAGTTGAAGGATAGACAGTTAAGGATCGAGGATGCTTTGAATGCATCAAAGGTGTACAGTTGGTCTGTTGTCCTTGAGAACATGAGTTCCAAATATCAGTTATAACCTTATGTTTGTACTTCGTTGTAGGCTGCTATTGAAGAAGGTGTTGTTGTTGGTGGAGGTTGTTGTCTTTTAAGGCTGTCTACAAAGGTGGATGCTATAAAAAATGTCCTGGAAAATGACGAGCAGATGGTATTACCCCTCTCGGTTTACCCCAAAATTTTCTGTTATAATTTTCTAATATTAAATATCAAGTTTGCTAGATTATGATGATTACAGGGAAATTTAGTTTTACTTAGCATATCAACTAGAATATCACGTTAATACGTATGATGGATTCTCTAATTTGTTTATATTAATTTCACTGGTCAATTGCAGGTTGGAGCCGAAATTTTCAAAAGGGCTTTGAGTTATCCTACAAAACTAATAGCGAGAAATGCCGGTGTCAATGGAAGTGTTGTTATAGATAAGGTCGCTGTGCACAAAGGAATTTGCTTCTAATTTACGGTGTACAGTTTTTCTCTCTGTCGAGGCTAATCAATCATGGTAAAATTAACTTTCTATCTCACAGATTCTAAGTAACAATGATCTGGATTATGGATATAATGCCGCCACAGACCGTTACGAGGATCTAATGAAAGCTGGTATCATGGATCCATCAAAGGTTTATTAACTTTGTTCAGAGCTCTTTGACAGTCTATGCTTTACCATTCCAAATAGTTTTTAAGGATTTCCGAATTACAGAAAAGCATCCATCTTTTGGTGAAACCTGAAATTTTTAGCAGCTAAAGGAACTTGGAAAAATGTGGATTGATAGAGGTTGATTGAAAATATATTCCATCAGCCAACAATTCTTGTCCTAACTCTTTAGTTCTCTACTGAAATCTGGGACAGGTTGTTAGATGTTGCCTGGAGCATGCAGCTTCTGTTTCGAAAACTTTCCTGACATCCGATGCTGTTGTAGTTGATATGAAGGAACCACAGCTCATCCCAAGAAGAACACCGATGCCAATCTCAGGTAAATGGGTTAAACAATATACATGCCTTTATTCTCTGATATCAATTCGTTTTGATTAACAAGCCTGCCTTTTGATGTTATAGGAGGCATCGGACAATTGGGTCTCTAG

mRNA sequence

ATGGCTACTCCCATTCGCCGGCATATCTTCCTCTTATCTTCATCCTTCACTGCGAGTACCAGAACTATGGCGTCTTCTCCCACTCCCATTTCTCCACTATCTTTTACCAATCCAATATCAACATCAAGAACGAAACCAAAACCATTACCATTTCCAAACAAACCGCCGTCTTCATTGCCTCCTGCGTTGAACTCCACCCCCAAACCTCCGCCCAAGGACCTTTACTTCAACCACGATGGCTCTACTTTTAAAAAACTTCAGGTCGGAGTTAATTTGGTAGCGGAGCTGGTGGGTATTACCTTGGGACCGAAGGGGAGGAATGTGGTGCTTCAGAATAAATATGGACCGCCTAAGATCGTCAACGATGGTGAAACCGTCCTGAAAGAGATTGAGCTGGAAGACCCTTTAGAGAACGTTGGGGTGAAATTGGTGAGACAAGCTGGTGCTAAGACGAATGACCTTGCTGGTGATGGTTCCACTACATCTGTCGTTCTTGCCCAAGGATTGATTGCTGAGGGTATGAAGGTTATTGCATCTGGCATGAATCCTGTTCAAATTGCACGTGGGATTGAGAAGACTGCAAAAGCACTAGTTTCTGAGCTGAAATTGATGTCAAGAGAGGTTGAAGACCACGAGATAGCACACGTGGCCGCAGTTAGCGCAGGGAATGATTATGCTGTGGGAAGTTTGGTTTCAGATGCCTTACGTCAAGTTGGATGGAAGGGAGTCGTCCAAATTGAAAAAGGGAAGAGTGTGGATAATAGTTTACAAATTGTTGAAGGGATGCAATTTGATCGTGGATATTTATCCCCATACTTCGTGACTGATAGAAGGAAGATGGTAGTAGAATTCCATGAGTGCAAGTTACTGTTGGTCGACAAAAAAATTTCCGATCCAAAGGAGATGTTTAAAATATTGGACAATGCAGTGAAAGAGAAATACCCAATTGTGATATTGGCAGAGGGCATTGAGCCGGAAGCTCTGGCTCCAGTAATTAGGAATAAACTTAGAGGTGTGCTGAAGGCAGCCGCTATCAAGGCTCCTGCCTTTGGCGAGCGCAAGAGTCACTATTTAGATGACATAGCCACTTTAACTGGAGCTACCGTGGTCAGAGAAGATAATGGATTGACGTTAGAAAAGACAGGCAAGGAGGTATTGGGCTCCGCTTGTAAGGTTGTTATATCAAAAGATTCCACACTGATAGTTACAGATGGGAATACTCGGGAAGCTGTTCAGAAAAGGGTCTTACAAATTCAAAAGCTTATGGAGAACACTGAGGAAAAATTTCCGAAGAAGATATTGAATGAGAGAATTGCAAGATTATCTGGGCGAATTGCAATAATTCAGGTAGGAGCACAAACTGAAGTTGAGTTGAAGGATAGACAGTTAAGGATCGAGGATGCTTTGAATGCATCAAAGGCTGCTATTGAAGAAGGTGTTGTTGTTGGTGGAGGTTGTTGTCTTTTAAGGCTGTCTACAAAGGTGGATGCTATAAAAAATGTCCTGGAAAATGACGAGCAGATGGTTGGAGCCGAAATTTTCAAAAGGGCTTTGAGTTATCCTACAAAACTAATAGCGAGAAATGCCGGTGTCAATGGAAGTGTTGTTATAGATAAGATTCTAAGTAACAATGATCTGGATTATGGATATAATGCCGCCACAGACCGTTACGAGGATCTAATGAAAGCTGGTATCATGGATCCATCAAAGGTTGTTAGATGTTGCCTGGAGCATGCAGCTTCTGTTTCGAAAACTTTCCTGACATCCGATGCTGTTGTAGTTGATATGAAGGAACCACAGCTCATCCCAAGAAGAACACCGATGCCAATCTCAGGAGGCATCGGACAATTGGGTCTCTAG

Coding sequence (CDS)

ATGGCTACTCCCATTCGCCGGCATATCTTCCTCTTATCTTCATCCTTCACTGCGAGTACCAGAACTATGGCGTCTTCTCCCACTCCCATTTCTCCACTATCTTTTACCAATCCAATATCAACATCAAGAACGAAACCAAAACCATTACCATTTCCAAACAAACCGCCGTCTTCATTGCCTCCTGCGTTGAACTCCACCCCCAAACCTCCGCCCAAGGACCTTTACTTCAACCACGATGGCTCTACTTTTAAAAAACTTCAGGTCGGAGTTAATTTGGTAGCGGAGCTGGTGGGTATTACCTTGGGACCGAAGGGGAGGAATGTGGTGCTTCAGAATAAATATGGACCGCCTAAGATCGTCAACGATGGTGAAACCGTCCTGAAAGAGATTGAGCTGGAAGACCCTTTAGAGAACGTTGGGGTGAAATTGGTGAGACAAGCTGGTGCTAAGACGAATGACCTTGCTGGTGATGGTTCCACTACATCTGTCGTTCTTGCCCAAGGATTGATTGCTGAGGGTATGAAGGTTATTGCATCTGGCATGAATCCTGTTCAAATTGCACGTGGGATTGAGAAGACTGCAAAAGCACTAGTTTCTGAGCTGAAATTGATGTCAAGAGAGGTTGAAGACCACGAGATAGCACACGTGGCCGCAGTTAGCGCAGGGAATGATTATGCTGTGGGAAGTTTGGTTTCAGATGCCTTACGTCAAGTTGGATGGAAGGGAGTCGTCCAAATTGAAAAAGGGAAGAGTGTGGATAATAGTTTACAAATTGTTGAAGGGATGCAATTTGATCGTGGATATTTATCCCCATACTTCGTGACTGATAGAAGGAAGATGGTAGTAGAATTCCATGAGTGCAAGTTACTGTTGGTCGACAAAAAAATTTCCGATCCAAAGGAGATGTTTAAAATATTGGACAATGCAGTGAAAGAGAAATACCCAATTGTGATATTGGCAGAGGGCATTGAGCCGGAAGCTCTGGCTCCAGTAATTAGGAATAAACTTAGAGGTGTGCTGAAGGCAGCCGCTATCAAGGCTCCTGCCTTTGGCGAGCGCAAGAGTCACTATTTAGATGACATAGCCACTTTAACTGGAGCTACCGTGGTCAGAGAAGATAATGGATTGACGTTAGAAAAGACAGGCAAGGAGGTATTGGGCTCCGCTTGTAAGGTTGTTATATCAAAAGATTCCACACTGATAGTTACAGATGGGAATACTCGGGAAGCTGTTCAGAAAAGGGTCTTACAAATTCAAAAGCTTATGGAGAACACTGAGGAAAAATTTCCGAAGAAGATATTGAATGAGAGAATTGCAAGATTATCTGGGCGAATTGCAATAATTCAGGTAGGAGCACAAACTGAAGTTGAGTTGAAGGATAGACAGTTAAGGATCGAGGATGCTTTGAATGCATCAAAGGCTGCTATTGAAGAAGGTGTTGTTGTTGGTGGAGGTTGTTGTCTTTTAAGGCTGTCTACAAAGGTGGATGCTATAAAAAATGTCCTGGAAAATGACGAGCAGATGGTTGGAGCCGAAATTTTCAAAAGGGCTTTGAGTTATCCTACAAAACTAATAGCGAGAAATGCCGGTGTCAATGGAAGTGTTGTTATAGATAAGATTCTAAGTAACAATGATCTGGATTATGGATATAATGCCGCCACAGACCGTTACGAGGATCTAATGAAAGCTGGTATCATGGATCCATCAAAGGTTGTTAGATGTTGCCTGGAGCATGCAGCTTCTGTTTCGAAAACTTTCCTGACATCCGATGCTGTTGTAGTTGATATGAAGGAACCACAGCTCATCCCAAGAAGAACACCGATGCCAATCTCAGGAGGCATCGGACAATTGGGTCTCTAG

Protein sequence

MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLPPALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPISGGIGQLGL*
Homology
BLAST of CSPI05G24610 vs. ExPASy Swiss-Prot
Match: Q9C667 (Chaperonin 60 subunit beta 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60B4 PE=1 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 1.3e-223
Identity = 406/590 (68.81%), Postives = 495/590 (83.90%), Query Frame = 0

Query: 23  MASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLPPALNSTPKPPPKDLYFNHDGST 82
           MA S   +S L    P+S    + KP        SS  P      +   K+++FN DGS 
Sbjct: 1   MAFSQAALSAL----PLSDRTFRKKP-----SSSSSSSPNFVLRVRAAAKEVHFNRDGSV 60

Query: 83  FKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVK 142
            KKLQ G ++VA+L+G+TLGPKGRNVVLQNKYGPP+IVNDGETVLKEIELEDPLENVGVK
Sbjct: 61  TKKLQAGADMVAKLLGVTLGPKGRNVVLQNKYGPPRIVNDGETVLKEIELEDPLENVGVK 120

Query: 143 LVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSELK 202
           LVRQAGAKTNDLAGDGSTTS++LA GLI EG+KVI++G NP+Q+ARGIEKT KALV ELK
Sbjct: 121 LVRQAGAKTNDLAGDGSTTSIILAHGLITEGIKVISAGTNPIQVARGIEKTTKALVLELK 180

Query: 203 LMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKSVDNSLQIVEGM 262
            MSRE+EDHE+AHVAAVSAGNDY VG+++S+A +QVG  GVV IEKGK + N+L+IVEGM
Sbjct: 181 SMSREIEDHELAHVAAVSAGNDYEVGNMISNAFQQVGRTGVVTIEKGKYLVNNLEIVEGM 240

Query: 263 QFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVKEKYPIVILAEG 322
           QF+RGYLSPYFVTDRRK   EFH+CKLLLVDKKI++PK+MFKILD+AVKE++P++I+AE 
Sbjct: 241 QFNRGYLSPYFVTDRRKREAEFHDCKLLLVDKKITNPKDMFKILDSAVKEEFPVLIVAED 300

Query: 323 IEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEKTG 382
           IE +ALAPVIRNKL+G LK AAIKAPAFGERKSH LDD+A  TGATV+R++ GL+LEK G
Sbjct: 301 IEQDALAPVIRNKLKGNLKVAAIKAPAFGERKSHCLDDLAIFTGATVIRDEMGLSLEKAG 360

Query: 383 KEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLS 442
           KEVLG+A +V+++KDSTLIVT+G T++AV +RV QI+ L+ENTEE F KKILNER+ARLS
Sbjct: 361 KEVLGTAKRVLVTKDSTLIVTNGFTQKAVDERVSQIKNLIENTEENFQKKILNERVARLS 420

Query: 443 GRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL 502
           G IAIIQVGA T+VELKD+QL++EDALNA+K+AIEEG+VVGGGC LLRL+TKVD IK  L
Sbjct: 421 GGIAIIQVGALTQVELKDKQLKVEDALNATKSAIEEGIVVGGGCALLRLATKVDRIKETL 480

Query: 503 ENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYEDLMK 562
           +N EQ +GAEIFK+ALSYP +LIA+NA  NG++VI+K+LSN +  YGYNAA ++YEDLM 
Sbjct: 481 DNTEQKIGAEIFKKALSYPIRLIAKNADTNGNIVIEKVLSNKNTMYGYNAAKNQYEDLML 540

Query: 563 AGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIP-RRTPMPIS 612
           AGI+DP+KVVRCCLEHA+SV++TFLTSD VVV++KE +  P    P+P S
Sbjct: 541 AGIIDPTKVVRCCLEHASSVAQTFLTSDCVVVEIKEIKPRPIINPPLPTS 581

BLAST of CSPI05G24610 vs. ExPASy Swiss-Prot
Match: Q9LJE4 (Chaperonin 60 subunit beta 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60B2 PE=1 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 3.1e-214
Identity = 370/541 (68.39%), Postives = 467/541 (86.32%), Query Frame = 0

Query: 72  KDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIE 131
           K+L+FN DG+T +KLQ GVN +A+LVG+TLGPKGRNVVL++KYG P+IVNDG TV +E+E
Sbjct: 53  KELHFNKDGTTIRKLQTGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVE 112

Query: 132 LEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIE 191
           LEDP+EN+G KLVRQA AKTNDLAGDG+TTSVVLAQG IAEG+KV+A+G NPV I RGIE
Sbjct: 113 LEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIE 172

Query: 192 KTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKS 251
           KTAKALV+ELKLMS+EVED E+A VAAVSAGN++ VGS++++A+ +VG KGVV +E+GKS
Sbjct: 173 KTAKALVNELKLMSKEVEDSELADVAAVSAGNNHEVGSMIAEAMSKVGRKGVVTLEEGKS 232

Query: 252 VDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVK 311
            +N+L +VEGMQFDRGY+SPYFVTD  KM VE+  CKLLLVDKK+++ +++  +L++A++
Sbjct: 233 AENNLYVVEGMQFDRGYISPYFVTDSEKMSVEYDNCKLLLVDKKVTNARDLVGVLEDAIR 292

Query: 312 EKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVR 371
             YPI+I+AE IE EALA ++ NKLRG LK AA+KAP FGERKS YLDDIA LTGATV+R
Sbjct: 293 GGYPILIIAEDIEQEALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATVIR 352

Query: 372 EDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPK 431
           E+ GL+L+K GKEVLG+A KVV++K+ T IV DG T+EAV KRV+QI+ L+E  E+ + K
Sbjct: 353 EEVGLSLDKAGKEVLGNASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEK 412

Query: 432 KILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRL 491
           + LNERIA+LSG +A+IQVGAQTE ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLRL
Sbjct: 413 EKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRL 472

Query: 492 STKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYN 551
           ++KVDAIK+ LENDE+ VGAEI KRALSYP KLIA+NAGVNGSVV +K+L+N+++ +GYN
Sbjct: 473 ASKVDAIKDTLENDEEKVGAEIVKRALSYPLKLIAKNAGVNGSVVSEKVLANDNVKFGYN 532

Query: 552 AATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPIS 611
           AAT +YEDLM AGI+DP+KVVRCCLEHAASV+KTFL SD VVV++ EP+ +P   PM  S
Sbjct: 533 AATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKTFLMSDCVVVEIPEPEPVPAGNPMDNS 592

Query: 612 G 613
           G
Sbjct: 593 G 593

BLAST of CSPI05G24610 vs. ExPASy Swiss-Prot
Match: P21240 (Chaperonin 60 subunit beta 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60B1 PE=1 SV=3)

HSP 1 Score: 736.9 bits (1901), Expect = 1.9e-211
Identity = 365/541 (67.47%), Postives = 467/541 (86.32%), Query Frame = 0

Query: 72  KDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIE 131
           K+L+FN DG+T ++LQ GVN +A+LVG+TLGPKGRNVVL++KYG P+IVNDG TV +E+E
Sbjct: 57  KELHFNKDGTTIRRLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVE 116

Query: 132 LEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIE 191
           LEDP+EN+G KLVRQA AKTNDLAGDG+TTSVVLAQG IAEG+KV+A+G NPV I RGIE
Sbjct: 117 LEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIE 176

Query: 192 KTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKS 251
           KTAKALV+ELK MS+EVED E+A VAAVSAGN+  +G+++++A+ +VG KGVV +E+GKS
Sbjct: 177 KTAKALVTELKKMSKEVEDSELADVAAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKS 236

Query: 252 VDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVK 311
            +N+L +VEGMQFDRGY+SPYFVTD  KM VEF  CKLLLVDKKI++ +++  +L++A++
Sbjct: 237 AENNLYVVEGMQFDRGYISPYFVTDSEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIR 296

Query: 312 EKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVR 371
             YPI+I+AE IE EALA ++ NKLRG LK AA++AP FGERKS YLDDIA LTGATV+R
Sbjct: 297 GGYPILIIAEDIEQEALATLVVNKLRGTLKIAALRAPGFGERKSQYLDDIAILTGATVIR 356

Query: 372 EDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPK 431
           E+ GL+L+K GKEVLG+A KVV++K+++ IV DG+T++AV+KRV QI+ L+E  E+ + K
Sbjct: 357 EEVGLSLDKAGKEVLGNASKVVLTKETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEK 416

Query: 432 KILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRL 491
           + LNERIA+LSG +A+IQVGAQTE ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLRL
Sbjct: 417 EKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRL 476

Query: 492 STKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYN 551
           ++KVDAIK  L+NDE+ VGA+I KRALSYP KLIA+NAGVNGSVV +K+LSN+++ +GYN
Sbjct: 477 ASKVDAIKATLDNDEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYN 536

Query: 552 AATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPIS 611
           AAT +YEDLM AGI+DP+KVVRCCLEHAASV+KTFL SD VVV++KEP+ +P   PM  S
Sbjct: 537 AATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKTFLMSDCVVVEIKEPEPVPVGNPMDNS 596

Query: 612 G 613
           G
Sbjct: 597 G 597

BLAST of CSPI05G24610 vs. ExPASy Swiss-Prot
Match: P08927 (RuBisCO large subunit-binding protein subunit beta, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=2)

HSP 1 Score: 731.5 bits (1887), Expect = 8.0e-210
Identity = 387/602 (64.29%), Postives = 489/602 (81.23%), Query Frame = 0

Query: 12  LSSSFTASTRTM-ASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLPPALNSTPKPP 71
           ++S+F+A+T +   SS   IS  SF  P++  +     +  P K  +    A+       
Sbjct: 1   MASTFSATTSSCNLSSSAAIS--SF--PLAAGKRNANKVVLPRKNRNVKVSAM------- 60

Query: 72  PKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEI 131
            K+L+FN DGS  KKLQ GVN +A+LVG+TLGPKGRNVVL++KYG PKIVNDG TV KE+
Sbjct: 61  AKELHFNKDGSAIKKLQNGVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAKEV 120

Query: 132 ELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGI 191
           ELEDP+EN+G KLVRQA AKTNDLAGDG+TTSVVLAQGLIAEG+KV+A+G NPV I RGI
Sbjct: 121 ELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGI 180

Query: 192 EKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGK 251
           EKT+KALV+ELK MS+EVED E+A VAAVSAGN++ VG+++++AL +VG KGVV +E+GK
Sbjct: 181 EKTSKALVAELKKMSKEVEDSELADVAAVSAGNNHEVGNMIAEALSKVGRKGVVTLEEGK 240

Query: 252 SVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAV 311
           S +NSL +VEGMQFDRGY+SPYFVTD  KM VEF  CKLLLVDKKI++ +++  IL++A+
Sbjct: 241 SAENSLYVVEGMQFDRGYISPYFVTDSEKMTVEFENCKLLLVDKKITNARDLINILEDAI 300

Query: 312 KEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVV 371
           +  +PIVI+AE IE EALA ++ NKLRG LK AA+KAP FGERKS YLDDIA LTG TV+
Sbjct: 301 RSGFPIVIIAEDIEQEALATLVVNKLRGSLKIAALKAPGFGERKSQYLDDIAILTGGTVI 360

Query: 372 REDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFP 431
           RE+ GLTL+K  KEVLG+A KVV++KD+T IV DG+T+EAV KRV QI+  +E  E+++ 
Sbjct: 361 REEVGLTLDKADKEVLGNAAKVVLTKDTTTIVGDGSTQEAVNKRVSQIKNQIEAAEQEYE 420

Query: 432 KKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLR 491
           K+ L+ERIA+LSG +A+IQVGAQTE ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLR
Sbjct: 421 KEKLSERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLR 480

Query: 492 LSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGY 551
           L++KVDAIK+ L NDE+ VGA+I KRALSYP KLIA+NAGVNGSVV +K+LS+++  YGY
Sbjct: 481 LASKVDAIKDTLANDEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSSDNPKYGY 540

Query: 552 NAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPI 611
           NAAT +YEDLM AGI+DP+KVVRCCLEHA+SV+KTFL SD VVV++KEP+  P   PM  
Sbjct: 541 NAATGKYEDLMAAGIIDPTKVVRCCLEHASSVAKTFLMSDCVVVEIKEPESAPVGNPMDN 591

Query: 612 SG 613
           SG
Sbjct: 601 SG 591

BLAST of CSPI05G24610 vs. ExPASy Swiss-Prot
Match: P21241 (RuBisCO large subunit-binding protein subunit beta, chloroplastic OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 1.5e-208
Identity = 380/594 (63.97%), Postives = 487/594 (81.99%), Query Frame = 0

Query: 12  LSSSFTA--STRTMASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLPPALNSTPKP 71
           ++S+FTA  S  +M +     +     N +S+S    +   FP    SS  PA+      
Sbjct: 1   MASTFTATSSIGSMVAPNAHKTDKKLMNKLSSSSFGRRQNVFPKLRRSS--PAVVCA--- 60

Query: 72  PPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKE 131
             K+L+FN DG+T +KLQ GVN +A+LVG+TLGPKGRNVVL++KYG P+IVNDG TV +E
Sbjct: 61  -AKELHFNKDGTTIRKLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVARE 120

Query: 132 IELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARG 191
           +ELEDP+EN+G KLVRQA AKTNDLAGDG+TTSVVLAQG IAEG+KV+A+G NPV I RG
Sbjct: 121 VELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRG 180

Query: 192 IEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKG 251
           IEKTAKALV+ELK MS+EVED E+A VAAVSAGN+  +GS++++A+ +VG KGVV +E+G
Sbjct: 181 IEKTAKALVAELKKMSKEVEDSELADVAAVSAGNNAEIGSMIAEAMSRVGRKGVVTLEEG 240

Query: 252 KSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNA 311
           KS +N+L +VEGMQFDRGY+SPYFVTD  KM VEF  CKLLLVDKKI++ +++  +L++A
Sbjct: 241 KSAENALYVVEGMQFDRGYVSPYFVTDSEKMSVEFDNCKLLLVDKKITNARDLVGVLEDA 300

Query: 312 VKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATV 371
           ++  YPI+I+AE IE EALA ++ NKLRG LK AA+KAP FGERKS YLDDIA LTGATV
Sbjct: 301 IRGGYPILIIAEDIEQEALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATV 360

Query: 372 VREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKF 431
           +RE+ GL+L+K GKEVLG A KVV++K+++ IV DG+T++AVQKRV QI+ L+E  E+ +
Sbjct: 361 IREEVGLSLDKAGKEVLGHAAKVVLTKETSTIVGDGSTQDAVQKRVTQIKNLIEQAEQDY 420

Query: 432 PKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLL 491
            K+ LNERIA+LSG +A+IQVGAQTE ELK+++LR+EDALNA+KAA+EEG+VVGGGC LL
Sbjct: 421 EKEKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLL 480

Query: 492 RLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYG 551
           RL++KVDAIK  L+NDE+ VGA+I KRALSYP KLIA+NAGVNGSVV +K+LSN+++ +G
Sbjct: 481 RLASKVDAIKAALDNDEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFG 540

Query: 552 YNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIP 604
           YNAAT +YEDLM AGI+DP+KVVRCCLEHAASV+KTFL SD VVV++KEP+ +P
Sbjct: 541 YNAATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKTFLMSDCVVVEIKEPEPVP 588

BLAST of CSPI05G24610 vs. ExPASy TrEMBL
Match: A0A0A0KWB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G607990 PE=3 SV=1)

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 615/619 (99.35%), Postives = 616/619 (99.52%), Query Frame = 0

Query: 1   MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLP 60
           MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPK LPFPNKPPSSLP
Sbjct: 1   MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPKSLPFPNKPPSSLP 60

Query: 61  PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV 120
           PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV
Sbjct: 61  PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV 120

Query: 121 NDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASG 180
           NDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASG
Sbjct: 121 NDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASG 180

Query: 181 MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGW 240
           MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDA RQVGW
Sbjct: 181 MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDAFRQVGW 240

Query: 241 KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK 300
           KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK
Sbjct: 241 KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK 300

Query: 301 EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD 360
           EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD
Sbjct: 301 EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD 360

Query: 361 IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK 420
           IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK
Sbjct: 361 IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK 420

Query: 421 LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV 480
           LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV
Sbjct: 421 LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV 480

Query: 481 VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKI 540
           VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKI
Sbjct: 481 VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKI 540

Query: 541 LSNNDLDYGYNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQ 600
           LSNNDLDYGYNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKE Q
Sbjct: 541 LSNNDLDYGYNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKESQ 600

Query: 601 LIPRRTPMPISGGIGQLGL 620
           LIPRRTPMPISGG+GQLGL
Sbjct: 601 LIPRRTPMPISGGVGQLGL 619

BLAST of CSPI05G24610 vs. ExPASy TrEMBL
Match: A0A5D3CCJ6 (RuBisCO large subunit-binding protein subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00380 PE=3 SV=1)

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 583/599 (97.33%), Postives = 592/599 (98.83%), Query Frame = 0

Query: 23  MASSPTPISPLSFTNPISTSRTKPK--PLPFPNKPPSSLPPALNSTPKPPPKDLYFNHDG 82
           MASSP+PISPLSFTNPISTSRTKPK  PLPFPNKPPSS PPA N TPKPPPK+LYFNHDG
Sbjct: 1   MASSPSPISPLSFTNPISTSRTKPKPLPLPFPNKPPSSFPPASNPTPKPPPKELYFNHDG 60

Query: 83  STFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG 142
           STFKKLQ+GVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG
Sbjct: 61  STFKKLQIGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG 120

Query: 143 VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE 202
           VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE
Sbjct: 121 VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE 180

Query: 203 LKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKSVDNSLQIVE 262
           LKLMSREVEDHEIAHVAAVSAGNDYAVG+LVSDALRQVGWKGVVQIEKGKSVDNSLQIVE
Sbjct: 181 LKLMSREVEDHEIAHVAAVSAGNDYAVGNLVSDALRQVGWKGVVQIEKGKSVDNSLQIVE 240

Query: 263 GMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVKEKYPIVILA 322
           GMQFDRGYLSPYFVTDRRKMVVEFH+CKLLLVDKKISDPKEMFKILD+AVKEKYPIVILA
Sbjct: 241 GMQFDRGYLSPYFVTDRRKMVVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILA 300

Query: 323 EGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK 382
           EGIE EALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK
Sbjct: 301 EGIEQEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK 360

Query: 383 TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPKKILNERIAR 442
           TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRV+QIQKLMENTEEKFPKKILNERIAR
Sbjct: 361 TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVIQIQKLMENTEEKFPKKILNERIAR 420

Query: 443 LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN 502
           LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN
Sbjct: 421 LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN 480

Query: 503 VLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYEDL 562
           VLENDEQM+GAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYE+L
Sbjct: 481 VLENDEQMIGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYENL 540

Query: 563 MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPISGGIGQLGL 620
           MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKE QLIPRRTPMPISGGIGQLGL
Sbjct: 541 MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKESQLIPRRTPMPISGGIGQLGL 599

BLAST of CSPI05G24610 vs. ExPASy TrEMBL
Match: A0A1S3BEP6 (ruBisCO large subunit-binding protein subunit beta, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489212 PE=3 SV=1)

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 583/599 (97.33%), Postives = 592/599 (98.83%), Query Frame = 0

Query: 23  MASSPTPISPLSFTNPISTSRTKPK--PLPFPNKPPSSLPPALNSTPKPPPKDLYFNHDG 82
           MASSP+PISPLSFTNPISTSRTKPK  PLPFPNKPPSS PPA N TPKPPPK+LYFNHDG
Sbjct: 1   MASSPSPISPLSFTNPISTSRTKPKPLPLPFPNKPPSSFPPASNPTPKPPPKELYFNHDG 60

Query: 83  STFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG 142
           STFKKLQ+GVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG
Sbjct: 61  STFKKLQIGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG 120

Query: 143 VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE 202
           VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE
Sbjct: 121 VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE 180

Query: 203 LKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKSVDNSLQIVE 262
           LKLMSREVEDHEIAHVAAVSAGNDYAVG+LVSDALRQVGWKGVVQIEKGKSVDNSLQIVE
Sbjct: 181 LKLMSREVEDHEIAHVAAVSAGNDYAVGNLVSDALRQVGWKGVVQIEKGKSVDNSLQIVE 240

Query: 263 GMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVKEKYPIVILA 322
           GMQFDRGYLSPYFVTDRRKMVVEFH+CKLLLVDKKISDPKEMFKILD+AVKEKYPIVILA
Sbjct: 241 GMQFDRGYLSPYFVTDRRKMVVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILA 300

Query: 323 EGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK 382
           EGIE EALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK
Sbjct: 301 EGIEQEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK 360

Query: 383 TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPKKILNERIAR 442
           TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRV+QIQKLMENTEEKFPKKILNERIAR
Sbjct: 361 TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVIQIQKLMENTEEKFPKKILNERIAR 420

Query: 443 LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN 502
           LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN
Sbjct: 421 LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN 480

Query: 503 VLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYEDL 562
           VLENDEQM+GAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYE+L
Sbjct: 481 VLENDEQMIGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYENL 540

Query: 563 MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPISGGIGQLGL 620
           MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKE QLIPRRTPMPISGGIGQLGL
Sbjct: 541 MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKESQLIPRRTPMPISGGIGQLGL 599

BLAST of CSPI05G24610 vs. ExPASy TrEMBL
Match: A0A6J1G0R7 (chaperonin 60 subunit beta 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111449606 PE=3 SV=1)

HSP 1 Score: 1002.3 bits (2590), Expect = 9.0e-289
Identity = 525/597 (87.94%), Postives = 562/597 (94.14%), Query Frame = 0

Query: 23  MASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLPPALNSTPKPPPKDLYFNHDGST 82
           MASSPTPISPLSF N     R+KPK    PNKPP SLPP    +PK  PK++YFN DGS 
Sbjct: 1   MASSPTPISPLSFPN----RRSKPK----PNKPPPSLPPPWKPSPKSMPKEIYFNRDGSA 60

Query: 83  FKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVK 142
            KKLQVGV+LVAELVG+TLGPKGRNVVLQ KYGPPKIVNDGETVLKEIELED LENVGVK
Sbjct: 61  LKKLQVGVDLVAELVGVTLGPKGRNVVLQRKYGPPKIVNDGETVLKEIELEDRLENVGVK 120

Query: 143 LVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSELK 202
           LVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVI+SGM+PVQIARGIEKTA ALVSELK
Sbjct: 121 LVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELK 180

Query: 203 LMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKSVDNSLQIVEGM 262
           LMSREVEDHEIAHVAAVSAGNDYAVG+++SDALRQVG KGVVQIEKGKS+DNSLQIVEGM
Sbjct: 181 LMSREVEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGM 240

Query: 263 QFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVKEKYPIVILAEG 322
           QFD GYLSPYFVTDR+KM+VEFH+CKLLLVDKKISDPKEMFKILD+AVKEKYPIVILAEG
Sbjct: 241 QFDSGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEG 300

Query: 323 IEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEKTG 382
           IE  ALAPVIRNKLRGVLKAAA+KAPAFGERKSHYLDDIATLTGATV+RED+GLTLEKTG
Sbjct: 301 IEQAALAPVIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTG 360

Query: 383 KEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLS 442
           KEVLGSA KVVI+KDSTLIVTDG TREAVQKRVLQIQKL+ENTEEKFPKKILNERIARLS
Sbjct: 361 KEVLGSATKVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLS 420

Query: 443 GRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL 502
           GRIAI+QVGAQT+VELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL
Sbjct: 421 GRIAILQVGAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL 480

Query: 503 ENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYEDLMK 562
           EN+EQM+GAEIFKRALSYPT+LIARNAGVNGSVVIDK+L NND+++GYNAATD YEDLMK
Sbjct: 481 ENEEQMIGAEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNFGYNAATDHYEDLMK 540

Query: 563 AGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPISGGIGQLGL 620
           AGIMDPSKVVRCCLEHAASV+KTFLTSDAVVVD+KEP+  PRRTP+PIS GI ++GL
Sbjct: 541 AGIMDPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPESFPRRTPLPIS-GIKRMGL 588

BLAST of CSPI05G24610 vs. ExPASy TrEMBL
Match: A0A6J1HZU9 (chaperonin 60 subunit beta 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111467722 PE=3 SV=1)

HSP 1 Score: 998.4 bits (2580), Expect = 1.3e-287
Identity = 523/590 (88.64%), Postives = 556/590 (94.24%), Query Frame = 0

Query: 23  MASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLPPALNSTPKPPPKDLYFNHDGST 82
           MASSPTPISPLSF N     R+KPK    PNKPP SLPPA   +PK  PK++YFN DGS 
Sbjct: 1   MASSPTPISPLSFPN----RRSKPK----PNKPPPSLPPAWKPSPKSMPKEIYFNRDGSA 60

Query: 83  FKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVK 142
            KKLQVGV+LVAELVG+TLGPKGRNVVLQ+KYGPPKIVNDGETVLKEIELED LENVGVK
Sbjct: 61  LKKLQVGVDLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVK 120

Query: 143 LVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSELK 202
           LVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVI+SGM+PVQIARGIEKTA ALVSEL+
Sbjct: 121 LVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELE 180

Query: 203 LMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKSVDNSLQIVEGM 262
           LMSREVEDHEIAHVAAVSAGNDYAVG+++SDALRQVG KGVVQIEKGKS+DNSLQIVEGM
Sbjct: 181 LMSREVEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGM 240

Query: 263 QFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVKEKYPIVILAEG 322
           QFD GYLSPYFVTDR+KM+VEFH+CKLLLVDKKISDPKEMFKILD+AVKEKYPIVILAEG
Sbjct: 241 QFDSGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEG 300

Query: 323 IEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEKTG 382
            E  ALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATV+RED+GLTLEKTG
Sbjct: 301 FEQAALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTG 360

Query: 383 KEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLS 442
           KEVLGSA KVVI+KDSTLIVTDG TREAVQKRVLQIQKL+ENTEEKFPKKILNERIARLS
Sbjct: 361 KEVLGSATKVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLS 420

Query: 443 GRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL 502
           GRIAI+QVGAQT+VELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL
Sbjct: 421 GRIAILQVGAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL 480

Query: 503 ENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYEDLMK 562
           EN+EQM+GAEIFKRALSYPTKLIARNAGVNGSVVIDK+L NND++YGYNAATD YEDLMK
Sbjct: 481 ENEEQMIGAEIFKRALSYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMK 540

Query: 563 AGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPISG 613
           AGIMDPSKVVRCCLEHAASV+KTFLTSDAVVVDMKEP+  PRRT +P SG
Sbjct: 541 AGIMDPSKVVRCCLEHAASVAKTFLTSDAVVVDMKEPEPFPRRTTLPNSG 582

BLAST of CSPI05G24610 vs. NCBI nr
Match: XP_004135135.2 (ruBisCO large subunit-binding protein subunit beta, chloroplastic isoform X1 [Cucumis sativus] >KGN52011.1 hypothetical protein Csa_009336 [Cucumis sativus])

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 615/619 (99.35%), Postives = 616/619 (99.52%), Query Frame = 0

Query: 1   MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLP 60
           MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPK LPFPNKPPSSLP
Sbjct: 1   MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPKSLPFPNKPPSSLP 60

Query: 61  PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV 120
           PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV
Sbjct: 61  PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV 120

Query: 121 NDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASG 180
           NDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASG
Sbjct: 121 NDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASG 180

Query: 181 MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGW 240
           MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDA RQVGW
Sbjct: 181 MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDAFRQVGW 240

Query: 241 KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK 300
           KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK
Sbjct: 241 KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK 300

Query: 301 EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD 360
           EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD
Sbjct: 301 EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD 360

Query: 361 IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK 420
           IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK
Sbjct: 361 IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK 420

Query: 421 LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV 480
           LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV
Sbjct: 421 LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV 480

Query: 481 VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKI 540
           VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKI
Sbjct: 481 VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKI 540

Query: 541 LSNNDLDYGYNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQ 600
           LSNNDLDYGYNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKE Q
Sbjct: 541 LSNNDLDYGYNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKESQ 600

Query: 601 LIPRRTPMPISGGIGQLGL 620
           LIPRRTPMPISGG+GQLGL
Sbjct: 601 LIPRRTPMPISGGVGQLGL 619

BLAST of CSPI05G24610 vs. NCBI nr
Match: XP_008446493.1 (PREDICTED: ruBisCO large subunit-binding protein subunit beta, chloroplastic isoform X1 [Cucumis melo] >KAA0034496.1 ruBisCO large subunit-binding protein subunit beta [Cucumis melo var. makuwa] >TYK09050.1 ruBisCO large subunit-binding protein subunit beta [Cucumis melo var. makuwa])

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 583/599 (97.33%), Postives = 592/599 (98.83%), Query Frame = 0

Query: 23  MASSPTPISPLSFTNPISTSRTKPK--PLPFPNKPPSSLPPALNSTPKPPPKDLYFNHDG 82
           MASSP+PISPLSFTNPISTSRTKPK  PLPFPNKPPSS PPA N TPKPPPK+LYFNHDG
Sbjct: 1   MASSPSPISPLSFTNPISTSRTKPKPLPLPFPNKPPSSFPPASNPTPKPPPKELYFNHDG 60

Query: 83  STFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG 142
           STFKKLQ+GVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG
Sbjct: 61  STFKKLQIGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG 120

Query: 143 VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE 202
           VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE
Sbjct: 121 VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE 180

Query: 203 LKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKSVDNSLQIVE 262
           LKLMSREVEDHEIAHVAAVSAGNDYAVG+LVSDALRQVGWKGVVQIEKGKSVDNSLQIVE
Sbjct: 181 LKLMSREVEDHEIAHVAAVSAGNDYAVGNLVSDALRQVGWKGVVQIEKGKSVDNSLQIVE 240

Query: 263 GMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVKEKYPIVILA 322
           GMQFDRGYLSPYFVTDRRKMVVEFH+CKLLLVDKKISDPKEMFKILD+AVKEKYPIVILA
Sbjct: 241 GMQFDRGYLSPYFVTDRRKMVVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILA 300

Query: 323 EGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK 382
           EGIE EALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK
Sbjct: 301 EGIEQEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK 360

Query: 383 TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPKKILNERIAR 442
           TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRV+QIQKLMENTEEKFPKKILNERIAR
Sbjct: 361 TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVIQIQKLMENTEEKFPKKILNERIAR 420

Query: 443 LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN 502
           LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN
Sbjct: 421 LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN 480

Query: 503 VLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYEDL 562
           VLENDEQM+GAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYE+L
Sbjct: 481 VLENDEQMIGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYENL 540

Query: 563 MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPISGGIGQLGL 620
           MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKE QLIPRRTPMPISGGIGQLGL
Sbjct: 541 MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKESQLIPRRTPMPISGGIGQLGL 599

BLAST of CSPI05G24610 vs. NCBI nr
Match: XP_011655733.2 (chaperonin 60 subunit beta 4, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 1068.5 bits (2762), Expect = 2.1e-308
Identity = 569/619 (91.92%), Postives = 570/619 (92.08%), Query Frame = 0

Query: 1   MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLP 60
           MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPK LPFPNKPPSSLP
Sbjct: 1   MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPKSLPFPNKPPSSLP 60

Query: 61  PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV 120
           PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV
Sbjct: 61  PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV 120

Query: 121 NDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASG 180
           NDGETVLKE                                              VIASG
Sbjct: 121 NDGETVLKE----------------------------------------------VIASG 180

Query: 181 MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGW 240
           MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDA RQVGW
Sbjct: 181 MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDAFRQVGW 240

Query: 241 KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK 300
           KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK
Sbjct: 241 KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK 300

Query: 301 EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD 360
           EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD
Sbjct: 301 EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD 360

Query: 361 IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK 420
           IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK
Sbjct: 361 IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK 420

Query: 421 LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV 480
           LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV
Sbjct: 421 LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV 480

Query: 481 VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKI 540
           VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKI
Sbjct: 481 VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKI 540

Query: 541 LSNNDLDYGYNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQ 600
           LSNNDLDYGYNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKE Q
Sbjct: 541 LSNNDLDYGYNAATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKESQ 573

Query: 601 LIPRRTPMPISGGIGQLGL 620
           LIPRRTPMPISGG+GQLGL
Sbjct: 601 LIPRRTPMPISGGVGQLGL 573

BLAST of CSPI05G24610 vs. NCBI nr
Match: XP_038892006.1 (ruBisCO large subunit-binding protein subunit beta, chloroplastic [Benincasa hispida])

HSP 1 Score: 1056.6 bits (2731), Expect = 8.3e-305
Identity = 552/590 (93.56%), Postives = 573/590 (97.12%), Query Frame = 0

Query: 23  MASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLPPALNSTPKPPPKDLYFNHDGST 82
           MASSPTPI PLSF NP S SRTKP+P+  PN+PPSSLPPALN TPK PPK+LYFN DGST
Sbjct: 1   MASSPTPIPPLSFPNPTSRSRTKPRPI--PNRPPSSLPPALNPTPKSPPKELYFNRDGST 60

Query: 83  FKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVK 142
           FKKLQVGVNLVAELVG+TLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVK
Sbjct: 61  FKKLQVGVNLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVK 120

Query: 143 LVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSELK 202
           LVRQAGAKTNDLAGDGSTTSV+LAQGLIAEGMKVI+SGMNPVQIARGIEKTAKALVSELK
Sbjct: 121 LVRQAGAKTNDLAGDGSTTSVILAQGLIAEGMKVISSGMNPVQIARGIEKTAKALVSELK 180

Query: 203 LMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKSVDNSLQIVEGM 262
           L+SREVEDHEIAHVAAVSAGNDYAVG+LVS ALRQVG KGVVQIEKGKS+DNSLQIVEGM
Sbjct: 181 LLSREVEDHEIAHVAAVSAGNDYAVGNLVSHALRQVGRKGVVQIEKGKSIDNSLQIVEGM 240

Query: 263 QFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVKEKYPIVILAEG 322
           QFDRGYLSPYFVTDR+KMVVEFH+CKLLLVDKKISDPKEMFKILD+AVKEKYPIVILAEG
Sbjct: 241 QFDRGYLSPYFVTDRKKMVVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEG 300

Query: 323 IEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEKTG 382
           IE EALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGA VVRED+GLTLEKTG
Sbjct: 301 IEQEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGAIVVREDSGLTLEKTG 360

Query: 383 KEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLS 442
           KEVLGSA KVVISKDSTLIVTDGNTREAV+KRVLQIQKLMENTEEKFPKKILNERIARLS
Sbjct: 361 KEVLGSASKVVISKDSTLIVTDGNTREAVRKRVLQIQKLMENTEEKFPKKILNERIARLS 420

Query: 443 GRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL 502
           GRIAI+QVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL
Sbjct: 421 GRIAILQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL 480

Query: 503 ENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYEDLMK 562
           ENDEQM+GAEIFKRALSYPTKLIARNAGVNGSVVIDK+L NNDLDYGYNAATDRYEDLMK
Sbjct: 481 ENDEQMIGAEIFKRALSYPTKLIARNAGVNGSVVIDKVLINNDLDYGYNAATDRYEDLMK 540

Query: 563 AGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPISG 613
           AGIMDPSKVVRCCLEHAASV+KTFLTSDAVVVDMKEP+ I +RTPMPISG
Sbjct: 541 AGIMDPSKVVRCCLEHAASVAKTFLTSDAVVVDMKEPEPISKRTPMPISG 588

BLAST of CSPI05G24610 vs. NCBI nr
Match: XP_011655734.2 (chaperonin 60 subunit beta 4, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.9e-293
Identity = 537/540 (99.44%), Postives = 538/540 (99.63%), Query Frame = 0

Query: 1   MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLP 60
           MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPK LPFPNKPPSSLP
Sbjct: 1   MATPIRRHIFLLSSSFTASTRTMASSPTPISPLSFTNPISTSRTKPKSLPFPNKPPSSLP 60

Query: 61  PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV 120
           PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV
Sbjct: 61  PALNSTPKPPPKDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIV 120

Query: 121 NDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASG 180
           NDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASG
Sbjct: 121 NDGETVLKEIELEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASG 180

Query: 181 MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGW 240
           MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDA RQVGW
Sbjct: 181 MNPVQIARGIEKTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDAFRQVGW 240

Query: 241 KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK 300
           KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK
Sbjct: 241 KGVVQIEKGKSVDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPK 300

Query: 301 EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD 360
           EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD
Sbjct: 301 EMFKILDNAVKEKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDD 360

Query: 361 IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK 420
           IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK
Sbjct: 361 IATLTGATVVREDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQK 420

Query: 421 LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV 480
           LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV
Sbjct: 421 LMENTEEKFPKKILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGV 480

Query: 481 VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKI 540
           VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDK+
Sbjct: 481 VVGGGCCLLRLSTKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKV 540

BLAST of CSPI05G24610 vs. TAIR 10
Match: AT1G26230.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 777.3 bits (2006), Expect = 9.1e-225
Identity = 406/590 (68.81%), Postives = 495/590 (83.90%), Query Frame = 0

Query: 23  MASSPTPISPLSFTNPISTSRTKPKPLPFPNKPPSSLPPALNSTPKPPPKDLYFNHDGST 82
           MA S   +S L    P+S    + KP        SS  P      +   K+++FN DGS 
Sbjct: 1   MAFSQAALSAL----PLSDRTFRKKP-----SSSSSSSPNFVLRVRAAAKEVHFNRDGSV 60

Query: 83  FKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVK 142
            KKLQ G ++VA+L+G+TLGPKGRNVVLQNKYGPP+IVNDGETVLKEIELEDPLENVGVK
Sbjct: 61  TKKLQAGADMVAKLLGVTLGPKGRNVVLQNKYGPPRIVNDGETVLKEIELEDPLENVGVK 120

Query: 143 LVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSELK 202
           LVRQAGAKTNDLAGDGSTTS++LA GLI EG+KVI++G NP+Q+ARGIEKT KALV ELK
Sbjct: 121 LVRQAGAKTNDLAGDGSTTSIILAHGLITEGIKVISAGTNPIQVARGIEKTTKALVLELK 180

Query: 203 LMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKSVDNSLQIVEGM 262
            MSRE+EDHE+AHVAAVSAGNDY VG+++S+A +QVG  GVV IEKGK + N+L+IVEGM
Sbjct: 181 SMSREIEDHELAHVAAVSAGNDYEVGNMISNAFQQVGRTGVVTIEKGKYLVNNLEIVEGM 240

Query: 263 QFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVKEKYPIVILAEG 322
           QF+RGYLSPYFVTDRRK   EFH+CKLLLVDKKI++PK+MFKILD+AVKE++P++I+AE 
Sbjct: 241 QFNRGYLSPYFVTDRRKREAEFHDCKLLLVDKKITNPKDMFKILDSAVKEEFPVLIVAED 300

Query: 323 IEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEKTG 382
           IE +ALAPVIRNKL+G LK AAIKAPAFGERKSH LDD+A  TGATV+R++ GL+LEK G
Sbjct: 301 IEQDALAPVIRNKLKGNLKVAAIKAPAFGERKSHCLDDLAIFTGATVIRDEMGLSLEKAG 360

Query: 383 KEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLS 442
           KEVLG+A +V+++KDSTLIVT+G T++AV +RV QI+ L+ENTEE F KKILNER+ARLS
Sbjct: 361 KEVLGTAKRVLVTKDSTLIVTNGFTQKAVDERVSQIKNLIENTEENFQKKILNERVARLS 420

Query: 443 GRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVL 502
           G IAIIQVGA T+VELKD+QL++EDALNA+K+AIEEG+VVGGGC LLRL+TKVD IK  L
Sbjct: 421 GGIAIIQVGALTQVELKDKQLKVEDALNATKSAIEEGIVVGGGCALLRLATKVDRIKETL 480

Query: 503 ENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYEDLMK 562
           +N EQ +GAEIFK+ALSYP +LIA+NA  NG++VI+K+LSN +  YGYNAA ++YEDLM 
Sbjct: 481 DNTEQKIGAEIFKKALSYPIRLIAKNADTNGNIVIEKVLSNKNTMYGYNAAKNQYEDLML 540

Query: 563 AGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIP-RRTPMPIS 612
           AGI+DP+KVVRCCLEHA+SV++TFLTSD VVV++KE +  P    P+P S
Sbjct: 541 AGIIDPTKVVRCCLEHASSVAQTFLTSDCVVVEIKEIKPRPIINPPLPTS 581

BLAST of CSPI05G24610 vs. TAIR 10
Match: AT1G26230.2 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 763.1 bits (1969), Expect = 1.8e-220
Identity = 384/527 (72.87%), Postives = 468/527 (88.80%), Query Frame = 0

Query: 86  LQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVR 145
           ++ G ++VA+L+G+TLGPKGRNVVLQNKYGPP+IVNDGETVLKEIELEDPLENVGVKLVR
Sbjct: 3   VKAGADMVAKLLGVTLGPKGRNVVLQNKYGPPRIVNDGETVLKEIELEDPLENVGVKLVR 62

Query: 146 QAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSELKLMS 205
           QAGAKTNDLAGDGSTTS++LA GLI EG+KVI++G NP+Q+ARGIEKT KALV ELK MS
Sbjct: 63  QAGAKTNDLAGDGSTTSIILAHGLITEGIKVISAGTNPIQVARGIEKTTKALVLELKSMS 122

Query: 206 REVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKSVDNSLQIVEGMQFD 265
           RE+EDHE+AHVAAVSAGNDY VG+++S+A +QVG  GVV IEKGK + N+L+IVEGMQF+
Sbjct: 123 REIEDHELAHVAAVSAGNDYEVGNMISNAFQQVGRTGVVTIEKGKYLVNNLEIVEGMQFN 182

Query: 266 RGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVKEKYPIVILAEGIEP 325
           RGYLSPYFVTDRRK   EFH+CKLLLVDKKI++PK+MFKILD+AVKE++P++I+AE IE 
Sbjct: 183 RGYLSPYFVTDRRKREAEFHDCKLLLVDKKITNPKDMFKILDSAVKEEFPVLIVAEDIEQ 242

Query: 326 EALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEKTGKEV 385
           +ALAPVIRNKL+G LK AAIKAPAFGERKSH LDD+A  TGATV+R++ GL+LEK GKEV
Sbjct: 243 DALAPVIRNKLKGNLKVAAIKAPAFGERKSHCLDDLAIFTGATVIRDEMGLSLEKAGKEV 302

Query: 386 LGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPKKILNERIARLSGRI 445
           LG+A +V+++KDSTLIVT+G T++AV +RV QI+ L+ENTEE F KKILNER+ARLSG I
Sbjct: 303 LGTAKRVLVTKDSTLIVTNGFTQKAVDERVSQIKNLIENTEENFQKKILNERVARLSGGI 362

Query: 446 AIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLEND 505
           AIIQVGA T+VELKD+QL++EDALNA+K+AIEEG+VVGGGC LLRL+TKVD IK  L+N 
Sbjct: 363 AIIQVGALTQVELKDKQLKVEDALNATKSAIEEGIVVGGGCALLRLATKVDRIKETLDNT 422

Query: 506 EQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYEDLMKAGI 565
           EQ +GAEIFK+ALSYP +LIA+NA  NG++VI+K+LSN +  YGYNAA ++YEDLM AGI
Sbjct: 423 EQKIGAEIFKKALSYPIRLIAKNADTNGNIVIEKVLSNKNTMYGYNAAKNQYEDLMLAGI 482

Query: 566 MDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIP-RRTPMPIS 612
           +DP+KVVRCCLEHA+SV++TFLTSD VVV++KE +  P    P+P S
Sbjct: 483 IDPTKVVRCCLEHASSVAQTFLTSDCVVVEIKEIKPRPIINPPLPTS 529

BLAST of CSPI05G24610 vs. TAIR 10
Match: AT3G13470.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 746.1 bits (1925), Expect = 2.2e-215
Identity = 370/541 (68.39%), Postives = 467/541 (86.32%), Query Frame = 0

Query: 72  KDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIE 131
           K+L+FN DG+T +KLQ GVN +A+LVG+TLGPKGRNVVL++KYG P+IVNDG TV +E+E
Sbjct: 53  KELHFNKDGTTIRKLQTGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVE 112

Query: 132 LEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIE 191
           LEDP+EN+G KLVRQA AKTNDLAGDG+TTSVVLAQG IAEG+KV+A+G NPV I RGIE
Sbjct: 113 LEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIE 172

Query: 192 KTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKS 251
           KTAKALV+ELKLMS+EVED E+A VAAVSAGN++ VGS++++A+ +VG KGVV +E+GKS
Sbjct: 173 KTAKALVNELKLMSKEVEDSELADVAAVSAGNNHEVGSMIAEAMSKVGRKGVVTLEEGKS 232

Query: 252 VDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVK 311
            +N+L +VEGMQFDRGY+SPYFVTD  KM VE+  CKLLLVDKK+++ +++  +L++A++
Sbjct: 233 AENNLYVVEGMQFDRGYISPYFVTDSEKMSVEYDNCKLLLVDKKVTNARDLVGVLEDAIR 292

Query: 312 EKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVR 371
             YPI+I+AE IE EALA ++ NKLRG LK AA+KAP FGERKS YLDDIA LTGATV+R
Sbjct: 293 GGYPILIIAEDIEQEALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATVIR 352

Query: 372 EDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPK 431
           E+ GL+L+K GKEVLG+A KVV++K+ T IV DG T+EAV KRV+QI+ L+E  E+ + K
Sbjct: 353 EEVGLSLDKAGKEVLGNASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEK 412

Query: 432 KILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRL 491
           + LNERIA+LSG +A+IQVGAQTE ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLRL
Sbjct: 413 EKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRL 472

Query: 492 STKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYN 551
           ++KVDAIK+ LENDE+ VGAEI KRALSYP KLIA+NAGVNGSVV +K+L+N+++ +GYN
Sbjct: 473 ASKVDAIKDTLENDEEKVGAEIVKRALSYPLKLIAKNAGVNGSVVSEKVLANDNVKFGYN 532

Query: 552 AATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPIS 611
           AAT +YEDLM AGI+DP+KVVRCCLEHAASV+KTFL SD VVV++ EP+ +P   PM  S
Sbjct: 533 AATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKTFLMSDCVVVEIPEPEPVPAGNPMDNS 592

Query: 612 G 613
           G
Sbjct: 593 G 593

BLAST of CSPI05G24610 vs. TAIR 10
Match: AT1G55490.1 (chaperonin 60 beta )

HSP 1 Score: 736.9 bits (1901), Expect = 1.4e-212
Identity = 365/541 (67.47%), Postives = 467/541 (86.32%), Query Frame = 0

Query: 72  KDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIE 131
           K+L+FN DG+T ++LQ GVN +A+LVG+TLGPKGRNVVL++KYG P+IVNDG TV +E+E
Sbjct: 57  KELHFNKDGTTIRRLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVE 116

Query: 132 LEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIE 191
           LEDP+EN+G KLVRQA AKTNDLAGDG+TTSVVLAQG IAEG+KV+A+G NPV I RGIE
Sbjct: 117 LEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIE 176

Query: 192 KTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKS 251
           KTAKALV+ELK MS+EVED E+A VAAVSAGN+  +G+++++A+ +VG KGVV +E+GKS
Sbjct: 177 KTAKALVTELKKMSKEVEDSELADVAAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKS 236

Query: 252 VDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVK 311
            +N+L +VEGMQFDRGY+SPYFVTD  KM VEF  CKLLLVDKKI++ +++  +L++A++
Sbjct: 237 AENNLYVVEGMQFDRGYISPYFVTDSEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIR 296

Query: 312 EKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVR 371
             YPI+I+AE IE EALA ++ NKLRG LK AA++AP FGERKS YLDDIA LTGATV+R
Sbjct: 297 GGYPILIIAEDIEQEALATLVVNKLRGTLKIAALRAPGFGERKSQYLDDIAILTGATVIR 356

Query: 372 EDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPK 431
           E+ GL+L+K GKEVLG+A KVV++K+++ IV DG+T++AV+KRV QI+ L+E  E+ + K
Sbjct: 357 EEVGLSLDKAGKEVLGNASKVVLTKETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEK 416

Query: 432 KILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRL 491
           + LNERIA+LSG +A+IQVGAQTE ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLRL
Sbjct: 417 EKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRL 476

Query: 492 STKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYN 551
           ++KVDAIK  L+NDE+ VGA+I KRALSYP KLIA+NAGVNGSVV +K+LSN+++ +GYN
Sbjct: 477 ASKVDAIKATLDNDEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYN 536

Query: 552 AATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPIS 611
           AAT +YEDLM AGI+DP+KVVRCCLEHAASV+KTFL SD VVV++KEP+ +P   PM  S
Sbjct: 537 AATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKTFLMSDCVVVEIKEPEPVPVGNPMDNS 596

Query: 612 G 613
           G
Sbjct: 597 G 597

BLAST of CSPI05G24610 vs. TAIR 10
Match: AT1G55490.2 (chaperonin 60 beta )

HSP 1 Score: 736.9 bits (1901), Expect = 1.4e-212
Identity = 365/541 (67.47%), Postives = 467/541 (86.32%), Query Frame = 0

Query: 72  KDLYFNHDGSTFKKLQVGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIE 131
           K+L+FN DG+T ++LQ GVN +A+LVG+TLGPKGRNVVL++KYG P+IVNDG TV +E+E
Sbjct: 57  KELHFNKDGTTIRRLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVE 116

Query: 132 LEDPLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIE 191
           LEDP+EN+G KLVRQA AKTNDLAGDG+TTSVVLAQG IAEG+KV+A+G NPV I RGIE
Sbjct: 117 LEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIE 176

Query: 192 KTAKALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGSLVSDALRQVGWKGVVQIEKGKS 251
           KTAKALV+ELK MS+EVED E+A VAAVSAGN+  +G+++++A+ +VG KGVV +E+GKS
Sbjct: 177 KTAKALVTELKKMSKEVEDSELADVAAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKS 236

Query: 252 VDNSLQIVEGMQFDRGYLSPYFVTDRRKMVVEFHECKLLLVDKKISDPKEMFKILDNAVK 311
            +N+L +VEGMQFDRGY+SPYFVTD  KM VEF  CKLLLVDKKI++ +++  +L++A++
Sbjct: 237 AENNLYVVEGMQFDRGYISPYFVTDSEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIR 296

Query: 312 EKYPIVILAEGIEPEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVR 371
             YPI+I+AE IE EALA ++ NKLRG LK AA++AP FGERKS YLDDIA LTGATV+R
Sbjct: 297 GGYPILIIAEDIEQEALATLVVNKLRGTLKIAALRAPGFGERKSQYLDDIAILTGATVIR 356

Query: 372 EDNGLTLEKTGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVLQIQKLMENTEEKFPK 431
           E+ GL+L+K GKEVLG+A KVV++K+++ IV DG+T++AV+KRV QI+ L+E  E+ + K
Sbjct: 357 EEVGLSLDKAGKEVLGNASKVVLTKETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEK 416

Query: 432 KILNERIARLSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRL 491
           + LNERIA+LSG +A+IQVGAQTE ELK+++LR+EDALNA+KAA+EEG+VVGGGC LLRL
Sbjct: 417 EKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRL 476

Query: 492 STKVDAIKNVLENDEQMVGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYN 551
           ++KVDAIK  L+NDE+ VGA+I KRALSYP KLIA+NAGVNGSVV +K+LSN+++ +GYN
Sbjct: 477 ASKVDAIKATLDNDEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYN 536

Query: 552 AATDRYEDLMKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKEPQLIPRRTPMPIS 611
           AAT +YEDLM AGI+DP+KVVRCCLEHAASV+KTFL SD VVV++KEP+ +P   PM  S
Sbjct: 537 AATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKTFLMSDCVVVEIKEPEPVPVGNPMDNS 596

Query: 612 G 613
           G
Sbjct: 597 G 597

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6671.3e-22368.81Chaperonin 60 subunit beta 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LJE43.1e-21468.39Chaperonin 60 subunit beta 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=C... [more]
P212401.9e-21167.47Chaperonin 60 subunit beta 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=C... [more]
P089278.0e-21064.29RuBisCO large subunit-binding protein subunit beta, chloroplastic OS=Pisum sativ... [more]
P212411.5e-20863.97RuBisCO large subunit-binding protein subunit beta, chloroplastic OS=Brassica na... [more]
Match NameE-valueIdentityDescription
A0A0A0KWB10.0e+0099.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G607990 PE=3 SV=1[more]
A0A5D3CCJ60.0e+0097.33RuBisCO large subunit-binding protein subunit beta OS=Cucumis melo var. makuwa O... [more]
A0A1S3BEP60.0e+0097.33ruBisCO large subunit-binding protein subunit beta, chloroplastic isoform X1 OS=... [more]
A0A6J1G0R79.0e-28987.94chaperonin 60 subunit beta 4, chloroplastic-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1HZU91.3e-28788.64chaperonin 60 subunit beta 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
XP_004135135.20.0e+0099.35ruBisCO large subunit-binding protein subunit beta, chloroplastic isoform X1 [Cu... [more]
XP_008446493.10.0e+0097.33PREDICTED: ruBisCO large subunit-binding protein subunit beta, chloroplastic iso... [more]
XP_011655733.22.1e-30891.92chaperonin 60 subunit beta 4, chloroplastic isoform X2 [Cucumis sativus][more]
XP_038892006.18.3e-30593.56ruBisCO large subunit-binding protein subunit beta, chloroplastic [Benincasa his... [more]
XP_011655734.21.9e-29399.44chaperonin 60 subunit beta 4, chloroplastic isoform X3 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G26230.19.1e-22568.81TCP-1/cpn60 chaperonin family protein [more]
AT1G26230.21.8e-22072.87TCP-1/cpn60 chaperonin family protein [more]
AT3G13470.12.2e-21568.39TCP-1/cpn60 chaperonin family protein [more]
AT1G55490.11.4e-21267.47chaperonin 60 beta [more]
AT1G55490.21.4e-21267.47chaperonin 60 beta [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 408..428
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 48..68
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..78
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..47
NoneNo IPR availablePANTHERPTHR4563360 KDA HEAT SHOCK PROTEIN, MITOCHONDRIALcoord: 27..600
NoneNo IPR availablePANTHERPTHR45633:SF7CHAPERONIN 60 SUBUNIT BETA 4, CHLOROPLASTICcoord: 27..600
IPR001844Chaperonin Cpn60PRINTSPR00298CHAPERONIN60coord: 337..360
score: 51.82
coord: 97..123
score: 60.56
coord: 419..444
score: 33.5
coord: 467..488
score: 64.66
coord: 153..180
score: 53.22
IPR001844Chaperonin Cpn60TIGRFAMTIGR02348TIGR02348coord: 72..598
e-value: 1.8E-195
score: 648.3
IPR001844Chaperonin Cpn60CDDcd03344GroELcoord: 72..595
e-value: 0.0
score: 701.135
IPR027410TCP-1-like chaperonin intermediate domain superfamilyGENE3D3.30.260.10coord: 207..479
e-value: 1.7E-191
score: 639.1
IPR027410TCP-1-like chaperonin intermediate domain superfamilySUPERFAMILY54849GroEL-intermediate domain likecoord: 210..265
IPR027413GroEL-like equatorial domain superfamilyGENE3D1.10.560.10coord: 84..594
e-value: 1.7E-191
score: 639.1
IPR027413GroEL-like equatorial domain superfamilySUPERFAMILY48592GroEL equatorial domain-likecoord: 80..593
IPR027409GroEL-like apical domain superfamilyGENE3D3.50.7.10GroELcoord: 261..442
e-value: 1.7E-191
score: 639.1
IPR027409GroEL-like apical domain superfamilySUPERFAMILY52029GroEL apical domain-likecoord: 254..445
IPR002423Chaperonin Cpn60/TCP-1 familyPFAMPF00118Cpn60_TCP1coord: 93..596
e-value: 1.6E-103
score: 347.0
IPR018370Chaperonin Cpn60, conserved sitePROSITEPS00296CHAPERONINS_CPN60coord: 474..485

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G24610.1CSPI05G24610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042026 protein refolding
biological_process GO:0006457 protein folding
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity