CSPI05G24420 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G24420
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionprotein BIG GRAIN 1-like E
LocationChr5: 24050702 .. 24051705 (-)
RNA-Seq ExpressionCSPI05G24420
SyntenyCSPI05G24420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAAGTTTCATTTTCTTTCCATATATATTTGCCACTCCCCACTCCCACCTCTCCCACAAAATTCTTTCCTATCAAACCTCTCCGCCCAATTTCCCTCTCCGACTACACCATGTCCGTCACCAGTCACCTCTCCGGCCACCCAGACAAAATCTTCCGCAAATCTCTCCACCGCCGCAAAGATTCCGACGAACTCGATGTCTTCGAAGCCGCACGCTACTTCGCCGGCAGCAACGAACCCAGTTACACCACCGCTGCCACTTACGAAACTTCCTTATTTCACGGCGGCCGTAGAGGCGGAAGAATGAGCCTCGATTTACCACTAAGAACCAATGTCATTCCTCTTCCAACACCACCGTACACGGCGGAGAAACAATCGGTTAAAGACCCAAAAAAACACAGGCAACAACCCAGTTCCCCCGGCGGCCGCCTTGCCAATTTCCTGAATTCTCTGTTCAATCAATCTGCTTCGAAAAAGAAAAAGAAACCAAAAAATTCCATCAAAACCGAAGATCTTCATCACGAAATGGGTGCAAGAAAGAGAAGAAGCAGCTTAAGTACGTTGATTGATACAAAGTCATCGTCTCATAGTTCTTCAAAAATTTCGGGATTTAGGACTCCACCGGCTCCTAATTGCTGCACTGTTCAAACTCCGAATAAAAATTATATGGAAATTAGAAGCTTTTTGGATCAGAAAAGAGAAGGTATTAATTACTACTCGAAGAAAAATGGGATTAATAATCAAAAATCAGAGATGAGAAAAATGAACGATCAAGATGAAGGAGATGAGAGTGATTCAAGTTCTGATCTTTTTGAACTAAGGATTTGTGATCGTTTTGATTGTTATTCATCAAATGAATTGCCTGTGTATCGAACCACCAACTTTCAGACCATCAACAAACTGTAAACTAATTTCCTCTTTTTCATTATCCTTTTTTTTTCTTCTTAATATCAATTATTTGCTTCTTTTTTTCTTTCTTTCTTTTGGCTTAAATTATAAAATAC

mRNA sequence

AGAAAAGTTTCATTTTCTTTCCATATATATTTGCCACTCCCCACTCCCACCTCTCCCACAAAATTCTTTCCTATCAAACCTCTCCGCCCAATTTCCCTCTCCGACTACACCATGTCCGTCACCAGTCACCTCTCCGGCCACCCAGACAAAATCTTCCGCAAATCTCTCCACCGCCGCAAAGATTCCGACGAACTCGATGTCTTCGAAGCCGCACGCTACTTCGCCGGCAGCAACGAACCCAGTTACACCACCGCTGCCACTTACGAAACTTCCTTATTTCACGGCGGCCGTAGAGGCGGAAGAATGAGCCTCGATTTACCACTAAGAACCAATGTCATTCCTCTTCCAACACCACCGTACACGGCGGAGAAACAATCGGTTAAAGACCCAAAAAAACACAGGCAACAACCCAGTTCCCCCGGCGGCCGCCTTGCCAATTTCCTGAATTCTCTGTTCAATCAATCTGCTTCGAAAAAGAAAAAGAAACCAAAAAATTCCATCAAAACCGAAGATCTTCATCACGAAATGGGTGCAAGAAAGAGAAGAAGCAGCTTAAGTACGTTGATTGATACAAAGTCATCGTCTCATAGTTCTTCAAAAATTTCGGGATTTAGGACTCCACCGGCTCCTAATTGCTGCACTGTTCAAACTCCGAATAAAAATTATATGGAAATTAGAAGCTTTTTGGATCAGAAAAGAGAAGGTATTAATTACTACTCGAAGAAAAATGGGATTAATAATCAAAAATCAGAGATGAGAAAAATGAACGATCAAGATGAAGGAGATGAGAGTGATTCAAGTTCTGATCTTTTTGAACTAAGGATTTGTGATCGTTTTGATTGTTATTCATCAAATGAATTGCCTGTGTATCGAACCACCAACTTTCAGACCATCAACAAACTGTAAACTAATTTCCTCTTTTTCATTATCCTTTTTTTTTCTTCTTAATATCAATTATTTGCTTCTTTTTTTCTTTCTTTCTTTTGGCTTAAATTATAAAATAC

Coding sequence (CDS)

ATGTCCGTCACCAGTCACCTCTCCGGCCACCCAGACAAAATCTTCCGCAAATCTCTCCACCGCCGCAAAGATTCCGACGAACTCGATGTCTTCGAAGCCGCACGCTACTTCGCCGGCAGCAACGAACCCAGTTACACCACCGCTGCCACTTACGAAACTTCCTTATTTCACGGCGGCCGTAGAGGCGGAAGAATGAGCCTCGATTTACCACTAAGAACCAATGTCATTCCTCTTCCAACACCACCGTACACGGCGGAGAAACAATCGGTTAAAGACCCAAAAAAACACAGGCAACAACCCAGTTCCCCCGGCGGCCGCCTTGCCAATTTCCTGAATTCTCTGTTCAATCAATCTGCTTCGAAAAAGAAAAAGAAACCAAAAAATTCCATCAAAACCGAAGATCTTCATCACGAAATGGGTGCAAGAAAGAGAAGAAGCAGCTTAAGTACGTTGATTGATACAAAGTCATCGTCTCATAGTTCTTCAAAAATTTCGGGATTTAGGACTCCACCGGCTCCTAATTGCTGCACTGTTCAAACTCCGAATAAAAATTATATGGAAATTAGAAGCTTTTTGGATCAGAAAAGAGAAGGTATTAATTACTACTCGAAGAAAAATGGGATTAATAATCAAAAATCAGAGATGAGAAAAATGAACGATCAAGATGAAGGAGATGAGAGTGATTCAAGTTCTGATCTTTTTGAACTAAGGATTTGTGATCGTTTTGATTGTTATTCATCAAATGAATTGCCTGTGTATCGAACCACCAACTTTCAGACCATCAACAAACTGTAA

Protein sequence

MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEPSYTTAATYETSLFHGGRRGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLNSLFNQSASKKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQTPNKNYMEIRSFLDQKREGINYYSKKNGINNQKSEMRKMNDQDEGDESDSSSDLFELRICDRFDCYSSNELPVYRTTNFQTINKL*
Homology
BLAST of CSPI05G24420 vs. ExPASy Swiss-Prot
Match: Q93Z37 (Protein BIG GRAIN 1-like E OS=Arabidopsis thaliana OX=3702 GN=At1g69160 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.8e-24
Identity = 115/326 (35.28%), Postives = 153/326 (46.93%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRK-SLHRRKDSDELDVFEAARYF----AGSNEPSYTTAATYETSL 60
           MS+    S   DK+ R+ SL  +++S+ELDVFEAA YF    A S +  +T    Y  + 
Sbjct: 1   MSMKGISSAESDKLSRRISLTHKRNSEELDVFEAAVYFGYNEASSGDHGHTQKYGYNAAR 60

Query: 61  FHGGRR------GGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKK-----HRQQPSSPG 120
               RR      G R+SLDLP+R +           EK  V   K+       +QPSSPG
Sbjct: 61  EENPRRWGILGGGRRISLDLPIRCSEQVYHLQQDHHEKHEVTTIKERLGNVRHKQPSSPG 120

Query: 121 GRLANFLNSLFNQSASKK-KKKPKNSIKTEDLHHE-------MGARKRRSSLS------- 180
           G++A+FLNSLF+Q+ SKK K K K+  K  D   E          R+RRSS+S       
Sbjct: 121 GKIASFLNSLFHQAGSKKNKSKSKSKTKPTDPEVEEEIPGGGWMRRRRRSSISHFFSSSR 180

Query: 181 -------TLIDTKSSSHSSSKISGFRTPPAPNCCTVQTPNKNYMEI-------------- 240
                  T   + S S  SS  SGFRTPP      + TP KNY +               
Sbjct: 181 STSTTTTTTASSSSKSLISSSSSGFRTPPP----YLNTPTKNYKQFLNYTSATKQVGEEE 240

Query: 241 ------RSFLDQKREGINYYSKKNGINNQKSEMR-----KMNDQDEGDESDSSSDLFELR 264
                  S+LD+K + +   S+   I +   ++      K   +D+G ESDSSSDLFEL+
Sbjct: 241 TKTNKEYSWLDEKLKVMESLSENQRIWSDDEDIDDDRRIKREGEDDGMESDSSSDLFELQ 300

BLAST of CSPI05G24420 vs. ExPASy Swiss-Prot
Match: Q9LVK2 (Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.2e-06
Identity = 65/193 (33.68%), Postives = 82/193 (42.49%), Query Frame = 0

Query: 95  KHRQQPSSPGGRLANFLNSLFNQSAS--KKKKKPKNSIKTEDLHHEMGARKRRS-SLSTL 154
           K  +QP SPGGRLA FLNSLF  +A+  KK KK       E+ H         S S S L
Sbjct: 163 KKVKQPISPGGRLATFLNSLFTNAATNPKKHKKTTTVAVVEEPHSSSTCSSASSFSRSCL 222

Query: 155 IDTKSSSHSSSKI-------------SGFRTPPAPNCCTVQTPN--KNYMEIRSFLDQKR 214
             T SSS  S +              S F  P A N   +   N  K   E R  +   +
Sbjct: 223 SKTPSSSGKSKRSVRFCPVNVILDEDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAK 282

Query: 215 EGINYYSKKNGINNQKSEMRKMNDQDEGDESD----SSSDLFELRICDRFDCYS-SNELP 265
           + +  Y  KN     K     +N+ +E DE D    +SSDLFEL             ELP
Sbjct: 283 DLLRTYHNKN-----KVTTTNINNVEEDDEDDAASCASSDLFELENLSAIGIERYREELP 342

BLAST of CSPI05G24420 vs. ExPASy Swiss-Prot
Match: B8APC7 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. indica OX=39946 GN=BG1 PE=3 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 6.6e-04
Identity = 63/237 (26.58%), Postives = 97/237 (40.93%), Query Frame = 0

Query: 33  AARYFAGSNEPSYTTAATYETSLFHGGRRGGRMSLD----LPLRTNV---IPLPTPPYTA 92
           A  Y A +  P  TT+++ E S + G       S       P+RT V    P P P   A
Sbjct: 75  AGSYRARAPGPHATTSSSSECSSYGGFSSSEAESSHHRRLRPIRTTVPGGAPGPAPEKKA 134

Query: 93  EK--QSVKDPKKHRQQPSSPGGRLANFLNSLFNQSASKKKKKPKNSIKTEDLHHEMGARK 152
           +K   S++   +  ++P+SPG RLA FLNS+F  +  +    P ++    +      +  
Sbjct: 135 KKPGASIRAKLRDLRKPASPGARLAGFLNSIF--AGKRAPATPPSATAGAESACSTASSY 194

Query: 153 RRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQTPNKNYMEIRSFLDQKREGINYYS 212
            RS LS    T+  +  + +     T    +   V         ++  L Q+        
Sbjct: 195 SRSCLSKTPSTRGQAKRTVRFLDSDTESLASSTVVDRRRVPVEAVQQMLLQR-------- 254

Query: 213 KKNGINNQKSEMRKMNDQDEGDESDSSSDLFELR----ICDRFDCYSSNELPVYRTT 257
                      M   +D+D+ + SD+SSDLFEL     I      Y  +ELPVY TT
Sbjct: 255 -----------MEMESDEDDDESSDASSDLFELENFAAIAPAGAAY-RDELPVYETT 289

BLAST of CSPI05G24420 vs. ExPASy Swiss-Prot
Match: Q10R09 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 6.6e-04
Identity = 63/237 (26.58%), Postives = 97/237 (40.93%), Query Frame = 0

Query: 33  AARYFAGSNEPSYTTAATYETSLFHGGRRGGRMSLD----LPLRTNV---IPLPTPPYTA 92
           A  Y A +  P  TT+++ E S + G       S       P+RT V    P P P   A
Sbjct: 75  AGSYRARAPGPHATTSSSSECSSYGGFSSSEAESSHHRRLRPIRTTVPGGAPGPAPEKKA 134

Query: 93  EK--QSVKDPKKHRQQPSSPGGRLANFLNSLFNQSASKKKKKPKNSIKTEDLHHEMGARK 152
           +K   S++   +  ++P+SPG RLA FLNS+F  +  +    P ++    +      +  
Sbjct: 135 KKPGASIRAKLRDLRKPASPGARLAGFLNSIF--AGKRAPATPPSATAGAESACSTASSY 194

Query: 153 RRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQTPNKNYMEIRSFLDQKREGINYYS 212
            RS LS    T+  +  + +     T    +   V         ++  L Q+        
Sbjct: 195 SRSCLSKTPSTRGQAKRTVRFLDSDTESLASSTVVDRRRVPVEAVQQMLLQR-------- 254

Query: 213 KKNGINNQKSEMRKMNDQDEGDESDSSSDLFELR----ICDRFDCYSSNELPVYRTT 257
                      M   +D+D+ + SD+SSDLFEL     I      Y  +ELPVY TT
Sbjct: 255 -----------MEMESDEDDDESSDASSDLFELENFAAIAPAGAAY-RDELPVYETT 289

BLAST of CSPI05G24420 vs. ExPASy TrEMBL
Match: A0A0A0KSX8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G606790 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 3.4e-144
Identity = 263/264 (99.62%), Postives = 263/264 (99.62%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEPSYTTAATYETSLFHGGR 60
           MSVTSHLSGHPDKIFRKSLHRRKDS ELDVFEAARYFAGSNEPSYTTAATYETSLFHGGR
Sbjct: 1   MSVTSHLSGHPDKIFRKSLHRRKDSGELDVFEAARYFAGSNEPSYTTAATYETSLFHGGR 60

Query: 61  RGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLNSLFNQSAS 120
           RGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLNSLFNQSAS
Sbjct: 61  RGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLNSLFNQSAS 120

Query: 121 KKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQT 180
           KKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQT
Sbjct: 121 KKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQT 180

Query: 181 PNKNYMEIRSFLDQKREGINYYSKKNGINNQKSEMRKMNDQDEGDESDSSSDLFELRICD 240
           PNKNYMEIRSFLDQKREGINYYSKKNGINNQKSEMRKMNDQDEGDESDSSSDLFELRICD
Sbjct: 181 PNKNYMEIRSFLDQKREGINYYSKKNGINNQKSEMRKMNDQDEGDESDSSSDLFELRICD 240

Query: 241 RFDCYSSNELPVYRTTNFQTINKL 265
           RFDCYSSNELPVYRTTNFQTINKL
Sbjct: 241 RFDCYSSNELPVYRTTNFQTINKL 264

BLAST of CSPI05G24420 vs. ExPASy TrEMBL
Match: A0A5D3CB02 (Protein BIG GRAIN 1-like E OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00190 PE=3 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.4e-129
Identity = 244/269 (90.71%), Postives = 256/269 (95.17%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEP-SYTTAATYETSLFHGG 60
           MSVTSHLSGHP+KIFRKSLHRRKDSDELDVFEAA YF+GSNEP +Y TA TYETSLFHGG
Sbjct: 1   MSVTSHLSGHPNKIFRKSLHRRKDSDELDVFEAASYFSGSNEPCNYNTATTYETSLFHGG 60

Query: 61  RRGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLNSLFNQSA 120
           RRGGRMSLDLPLRTNVIPLP PPYTAEKQS+KDPKKHRQQPSSPGGRLANFLNSLFNQS 
Sbjct: 61  RRGGRMSLDLPLRTNVIPLPPPPYTAEKQSIKDPKKHRQQPSSPGGRLANFLNSLFNQSG 120

Query: 121 SKKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQ 180
           SKKKKKPKNSIKTEDLHHE+GARKRRSSLSTLIDTKSSS++SSK+SGFRTPPAPNCC+VQ
Sbjct: 121 SKKKKKPKNSIKTEDLHHEIGARKRRSSLSTLIDTKSSSNNSSKVSGFRTPPAPNCCSVQ 180

Query: 181 TPNKNYMEIRSFLDQKREGINYYSKK----NGINNQKSEMRKMNDQDEGDESDSSSDLFE 240
           TP+K YMEIRSFLDQKREG NYYSKK    NGI N+KSEMRKMND+DEGDESDSSSDLFE
Sbjct: 181 TPSKKYMEIRSFLDQKREG-NYYSKKNNRGNGI-NEKSEMRKMNDEDEGDESDSSSDLFE 240

Query: 241 LRICDRFDCYSSNELPVYRTTNFQTINKL 265
           LRICDRFDCYSSNELPVYRTTNFQTINKL
Sbjct: 241 LRICDRFDCYSSNELPVYRTTNFQTINKL 267

BLAST of CSPI05G24420 vs. ExPASy TrEMBL
Match: A0A1S4DWX1 (protein BIG GRAIN 1-like E OS=Cucumis melo OX=3656 GN=LOC107990779 PE=3 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.4e-129
Identity = 244/269 (90.71%), Postives = 256/269 (95.17%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEP-SYTTAATYETSLFHGG 60
           MSVTSHLSGHP+KIFRKSLHRRKDSDELDVFEAA YF+GSNEP +Y TA TYETSLFHGG
Sbjct: 1   MSVTSHLSGHPNKIFRKSLHRRKDSDELDVFEAASYFSGSNEPCNYNTATTYETSLFHGG 60

Query: 61  RRGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLNSLFNQSA 120
           RRGGRMSLDLPLRTNVIPLP PPYTAEKQS+KDPKKHRQQPSSPGGRLANFLNSLFNQS 
Sbjct: 61  RRGGRMSLDLPLRTNVIPLPPPPYTAEKQSIKDPKKHRQQPSSPGGRLANFLNSLFNQSG 120

Query: 121 SKKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQ 180
           SKKKKKPKNSIKTEDLHHE+GARKRRSSLSTLIDTKSSS++SSK+SGFRTPPAPNCC+VQ
Sbjct: 121 SKKKKKPKNSIKTEDLHHEIGARKRRSSLSTLIDTKSSSNNSSKVSGFRTPPAPNCCSVQ 180

Query: 181 TPNKNYMEIRSFLDQKREGINYYSKK----NGINNQKSEMRKMNDQDEGDESDSSSDLFE 240
           TP+K YMEIRSFLDQKREG NYYSKK    NGI N+KSEMRKMND+DEGDESDSSSDLFE
Sbjct: 181 TPSKKYMEIRSFLDQKREG-NYYSKKNNRGNGI-NEKSEMRKMNDEDEGDESDSSSDLFE 240

Query: 241 LRICDRFDCYSSNELPVYRTTNFQTINKL 265
           LRICDRFDCYSSNELPVYRTTNFQTINKL
Sbjct: 241 LRICDRFDCYSSNELPVYRTTNFQTINKL 267

BLAST of CSPI05G24420 vs. ExPASy TrEMBL
Match: A0A6J1IMQ2 (protein BIG GRAIN 1-like E OS=Cucurbita maxima OX=3661 GN=LOC111478430 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 5.0e-87
Identity = 197/275 (71.64%), Postives = 211/275 (76.73%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEPSYTTAATYETSLF---- 60
           MSVTS LSG P+KIF KSLHRRKDS ELDVFEAARYF+G NE  Y  AA+Y+TSLF    
Sbjct: 1   MSVTSQLSGKPNKIFNKSLHRRKDSGELDVFEAARYFSGCNELHYNAAASYDTSLFSQKI 60

Query: 61  ----HGGRRGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLN 120
                GG RGGRMSLDLP+RTN++PLP P    EKQ+ KD KKHRQQPSSPGGRLANFLN
Sbjct: 61  FREDDGGWRGGRMSLDLPIRTNIVPLPQP----EKQTTKDQKKHRQQPSSPGGRLANFLN 120

Query: 121 SLFNQSASKKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSS-HSSSKISGFRTPP 180
           SLFNQSASKKKKKPK  IKTEDL HE G RKRRSSLSTLID+KSSS +SSS ISGFRTPP
Sbjct: 121 SLFNQSASKKKKKPKTPIKTEDL-HENGGRKRRSSLSTLIDSKSSSIYSSSNISGFRTPP 180

Query: 181 APNCCTVQTPNKNYMEIRSFLDQKREGINYYSKKNGINNQKS---EMRKMNDQDEGDESD 240
           APN CTVQTPNKNY EIRS LD+KR      SK  G    K    ++RKMN  DEG ESD
Sbjct: 181 APN-CTVQTPNKNYKEIRSLLDKKRTS----SKNLGTGYHKQNPYDIRKMNGVDEGAESD 240

Query: 241 SSSDLFELRICDRFDCYSSNELPVYRTTNFQTINK 264
           SSSDLFELRICD  DC     LPVY +TN QTINK
Sbjct: 241 SSSDLFELRICDS-DC----GLPVYESTNIQTINK 260

BLAST of CSPI05G24420 vs. ExPASy TrEMBL
Match: A0A6J1GXU5 (protein BIG GRAIN 1-like E OS=Cucurbita moschata OX=3662 GN=LOC111458485 PE=3 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.6e-85
Identity = 198/279 (70.97%), Postives = 214/279 (76.70%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEPSY---TTAATYETSLF- 60
           MSVTS LSG P+KIF KSLHRRKDS ELDVFEAARYF+G NE  Y     AA+Y+TSLF 
Sbjct: 1   MSVTSQLSGKPNKIFNKSLHRRKDSGELDVFEAARYFSGYNELHYNAAAAAASYDTSLFS 60

Query: 61  -------HGGRRGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLAN 120
                   GG RGGRMSLDLP+RTN++PLP P    EKQ+ KD KKHRQQPSSPGGRLAN
Sbjct: 61  QKIFREDDGGWRGGRMSLDLPIRTNIVPLPQP----EKQTTKDQKKHRQQPSSPGGRLAN 120

Query: 121 FLNSLFNQSASKKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSS-HSSSKISGFR 180
           FLNSLFNQSASKKKKKPKN IK+EDL HE G RKRRSSLSTLID+KSSS +SSS ISGFR
Sbjct: 121 FLNSLFNQSASKKKKKPKNPIKSEDL-HENGGRKRRSSLSTLIDSKSSSIYSSSNISGFR 180

Query: 181 TPPAPNCCTVQTPNKNYMEIRSFLDQKREGINYYSKKN---GINNQKS-EMRKMNDQDEG 240
           TPPAPN CTVQTPNKNY EIRS LDQKR      S KN   G + Q   ++RKMN  DEG
Sbjct: 181 TPPAPN-CTVQTPNKNYKEIRSLLDQKRT-----SSKNLGSGYHKQNQYDIRKMNGVDEG 240

Query: 241 DESDSSSDLFELRICDRFDCYSSNELPVYRTTNFQTINK 264
            ESDSSSDLFELRICD  DC     LPVY +TN QTIN+
Sbjct: 241 AESDSSSDLFELRICDS-DC----GLPVYESTNIQTINR 263

BLAST of CSPI05G24420 vs. NCBI nr
Match: XP_011655719.1 (protein BIG GRAIN 1-like E [Cucumis sativus] >KGN51989.1 hypothetical protein Csa_007727 [Cucumis sativus])

HSP 1 Score: 520.8 bits (1340), Expect = 7.0e-144
Identity = 263/264 (99.62%), Postives = 263/264 (99.62%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEPSYTTAATYETSLFHGGR 60
           MSVTSHLSGHPDKIFRKSLHRRKDS ELDVFEAARYFAGSNEPSYTTAATYETSLFHGGR
Sbjct: 1   MSVTSHLSGHPDKIFRKSLHRRKDSGELDVFEAARYFAGSNEPSYTTAATYETSLFHGGR 60

Query: 61  RGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLNSLFNQSAS 120
           RGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLNSLFNQSAS
Sbjct: 61  RGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLNSLFNQSAS 120

Query: 121 KKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQT 180
           KKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQT
Sbjct: 121 KKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQT 180

Query: 181 PNKNYMEIRSFLDQKREGINYYSKKNGINNQKSEMRKMNDQDEGDESDSSSDLFELRICD 240
           PNKNYMEIRSFLDQKREGINYYSKKNGINNQKSEMRKMNDQDEGDESDSSSDLFELRICD
Sbjct: 181 PNKNYMEIRSFLDQKREGINYYSKKNGINNQKSEMRKMNDQDEGDESDSSSDLFELRICD 240

Query: 241 RFDCYSSNELPVYRTTNFQTINKL 265
           RFDCYSSNELPVYRTTNFQTINKL
Sbjct: 241 RFDCYSSNELPVYRTTNFQTINKL 264

BLAST of CSPI05G24420 vs. NCBI nr
Match: XP_016900220.1 (PREDICTED: protein BIG GRAIN 1-like E [Cucumis melo] >KAA0034479.1 protein BIG GRAIN 1-like E [Cucumis melo var. makuwa] >TYK09033.1 protein BIG GRAIN 1-like E [Cucumis melo var. makuwa])

HSP 1 Score: 472.2 bits (1214), Expect = 2.9e-129
Identity = 244/269 (90.71%), Postives = 256/269 (95.17%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEP-SYTTAATYETSLFHGG 60
           MSVTSHLSGHP+KIFRKSLHRRKDSDELDVFEAA YF+GSNEP +Y TA TYETSLFHGG
Sbjct: 1   MSVTSHLSGHPNKIFRKSLHRRKDSDELDVFEAASYFSGSNEPCNYNTATTYETSLFHGG 60

Query: 61  RRGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLNSLFNQSA 120
           RRGGRMSLDLPLRTNVIPLP PPYTAEKQS+KDPKKHRQQPSSPGGRLANFLNSLFNQS 
Sbjct: 61  RRGGRMSLDLPLRTNVIPLPPPPYTAEKQSIKDPKKHRQQPSSPGGRLANFLNSLFNQSG 120

Query: 121 SKKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPAPNCCTVQ 180
           SKKKKKPKNSIKTEDLHHE+GARKRRSSLSTLIDTKSSS++SSK+SGFRTPPAPNCC+VQ
Sbjct: 121 SKKKKKPKNSIKTEDLHHEIGARKRRSSLSTLIDTKSSSNNSSKVSGFRTPPAPNCCSVQ 180

Query: 181 TPNKNYMEIRSFLDQKREGINYYSKK----NGINNQKSEMRKMNDQDEGDESDSSSDLFE 240
           TP+K YMEIRSFLDQKREG NYYSKK    NGI N+KSEMRKMND+DEGDESDSSSDLFE
Sbjct: 181 TPSKKYMEIRSFLDQKREG-NYYSKKNNRGNGI-NEKSEMRKMNDEDEGDESDSSSDLFE 240

Query: 241 LRICDRFDCYSSNELPVYRTTNFQTINKL 265
           LRICDRFDCYSSNELPVYRTTNFQTINKL
Sbjct: 241 LRICDRFDCYSSNELPVYRTTNFQTINKL 267

BLAST of CSPI05G24420 vs. NCBI nr
Match: XP_038892897.1 (protein BIG GRAIN 1-like E [Benincasa hispida])

HSP 1 Score: 404.1 bits (1037), Expect = 9.6e-109
Identity = 213/273 (78.02%), Postives = 235/273 (86.08%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEPSYTTAATYETSLF---- 60
           MS+T HLSG+PDK+FRKSLHRRKDS ELDVFEAARYF+GSNE  YT A TYETSLF    
Sbjct: 1   MSITGHLSGNPDKLFRKSLHRRKDSGELDVFEAARYFSGSNELPYTAATTYETSLFSQKI 60

Query: 61  ----HGGRRGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLN 120
               HG RRGGRMSLDLP+RTNVIPLP PPYTAEKQS+KD KK RQQPSSPGGRLANFLN
Sbjct: 61  FREDHGDRRGGRMSLDLPIRTNVIPLPPPPYTAEKQSIKDQKKQRQQPSSPGGRLANFLN 120

Query: 121 SLFNQSASKKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSSHSSSKISGFRTPPA 180
           SLFNQS+SKKKKKPKN I+ EDLH E+G +KRRSSLSTLID+K SS +SSKISGFRTPPA
Sbjct: 121 SLFNQSSSKKKKKPKNPIRNEDLHQEIGGKKRRSSLSTLIDSKFSS-NSSKISGFRTPPA 180

Query: 181 PNCCTVQTPNKNYMEIRSFLDQKREGINYYSKKNGINNQKSEM-RKMNDQDEGDESDSSS 240
           PNCCTVQTP+KNY EIRSFLDQKR   +  +  NGI+ +KSEM RK+ND DEGDESDSSS
Sbjct: 181 PNCCTVQTPSKNYKEIRSFLDQKRAANSSKNLGNGIDKKKSEMWRKINDPDEGDESDSSS 240

Query: 241 DLFELRICDRFDCYSSNELPVYRTTNFQTINKL 265
           DLFELRI D+FDCYSS ELPVY+TTN QT+N+L
Sbjct: 241 DLFELRIYDKFDCYSSTELPVYQTTNIQTMNRL 272

BLAST of CSPI05G24420 vs. NCBI nr
Match: XP_022978456.1 (protein BIG GRAIN 1-like E [Cucurbita maxima])

HSP 1 Score: 330.9 bits (847), Expect = 1.0e-86
Identity = 197/275 (71.64%), Postives = 211/275 (76.73%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEPSYTTAATYETSLF---- 60
           MSVTS LSG P+KIF KSLHRRKDS ELDVFEAARYF+G NE  Y  AA+Y+TSLF    
Sbjct: 1   MSVTSQLSGKPNKIFNKSLHRRKDSGELDVFEAARYFSGCNELHYNAAASYDTSLFSQKI 60

Query: 61  ----HGGRRGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLANFLN 120
                GG RGGRMSLDLP+RTN++PLP P    EKQ+ KD KKHRQQPSSPGGRLANFLN
Sbjct: 61  FREDDGGWRGGRMSLDLPIRTNIVPLPQP----EKQTTKDQKKHRQQPSSPGGRLANFLN 120

Query: 121 SLFNQSASKKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSS-HSSSKISGFRTPP 180
           SLFNQSASKKKKKPK  IKTEDL HE G RKRRSSLSTLID+KSSS +SSS ISGFRTPP
Sbjct: 121 SLFNQSASKKKKKPKTPIKTEDL-HENGGRKRRSSLSTLIDSKSSSIYSSSNISGFRTPP 180

Query: 181 APNCCTVQTPNKNYMEIRSFLDQKREGINYYSKKNGINNQKS---EMRKMNDQDEGDESD 240
           APN CTVQTPNKNY EIRS LD+KR      SK  G    K    ++RKMN  DEG ESD
Sbjct: 181 APN-CTVQTPNKNYKEIRSLLDKKRTS----SKNLGTGYHKQNPYDIRKMNGVDEGAESD 240

Query: 241 SSSDLFELRICDRFDCYSSNELPVYRTTNFQTINK 264
           SSSDLFELRICD  DC     LPVY +TN QTINK
Sbjct: 241 SSSDLFELRICDS-DC----GLPVYESTNIQTINK 260

BLAST of CSPI05G24420 vs. NCBI nr
Match: XP_022956957.1 (protein BIG GRAIN 1-like E [Cucurbita moschata])

HSP 1 Score: 325.9 bits (834), Expect = 3.3e-85
Identity = 198/279 (70.97%), Postives = 214/279 (76.70%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRKSLHRRKDSDELDVFEAARYFAGSNEPSY---TTAATYETSLF- 60
           MSVTS LSG P+KIF KSLHRRKDS ELDVFEAARYF+G NE  Y     AA+Y+TSLF 
Sbjct: 1   MSVTSQLSGKPNKIFNKSLHRRKDSGELDVFEAARYFSGYNELHYNAAAAAASYDTSLFS 60

Query: 61  -------HGGRRGGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKKHRQQPSSPGGRLAN 120
                   GG RGGRMSLDLP+RTN++PLP P    EKQ+ KD KKHRQQPSSPGGRLAN
Sbjct: 61  QKIFREDDGGWRGGRMSLDLPIRTNIVPLPQP----EKQTTKDQKKHRQQPSSPGGRLAN 120

Query: 121 FLNSLFNQSASKKKKKPKNSIKTEDLHHEMGARKRRSSLSTLIDTKSSS-HSSSKISGFR 180
           FLNSLFNQSASKKKKKPKN IK+EDL HE G RKRRSSLSTLID+KSSS +SSS ISGFR
Sbjct: 121 FLNSLFNQSASKKKKKPKNPIKSEDL-HENGGRKRRSSLSTLIDSKSSSIYSSSNISGFR 180

Query: 181 TPPAPNCCTVQTPNKNYMEIRSFLDQKREGINYYSKKN---GINNQKS-EMRKMNDQDEG 240
           TPPAPN CTVQTPNKNY EIRS LDQKR      S KN   G + Q   ++RKMN  DEG
Sbjct: 181 TPPAPN-CTVQTPNKNYKEIRSLLDQKRT-----SSKNLGSGYHKQNQYDIRKMNGVDEG 240

Query: 241 DESDSSSDLFELRICDRFDCYSSNELPVYRTTNFQTINK 264
            ESDSSSDLFELRICD  DC     LPVY +TN QTIN+
Sbjct: 241 AESDSSSDLFELRICDS-DC----GLPVYESTNIQTINR 263

BLAST of CSPI05G24420 vs. TAIR 10
Match: AT1G69160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 173 Blast hits to 172 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 25; Fungi - 33; Plants - 84; Viruses - 2; Other Eukaryotes - 29 (source: NCBI BLink). )

HSP 1 Score: 112.8 bits (281), Expect = 4.1e-25
Identity = 115/326 (35.28%), Postives = 153/326 (46.93%), Query Frame = 0

Query: 1   MSVTSHLSGHPDKIFRK-SLHRRKDSDELDVFEAARYF----AGSNEPSYTTAATYETSL 60
           MS+    S   DK+ R+ SL  +++S+ELDVFEAA YF    A S +  +T    Y  + 
Sbjct: 1   MSMKGISSAESDKLSRRISLTHKRNSEELDVFEAAVYFGYNEASSGDHGHTQKYGYNAAR 60

Query: 61  FHGGRR------GGRMSLDLPLRTNVIPLPTPPYTAEKQSVKDPKK-----HRQQPSSPG 120
               RR      G R+SLDLP+R +           EK  V   K+       +QPSSPG
Sbjct: 61  EENPRRWGILGGGRRISLDLPIRCSEQVYHLQQDHHEKHEVTTIKERLGNVRHKQPSSPG 120

Query: 121 GRLANFLNSLFNQSASKK-KKKPKNSIKTEDLHHE-------MGARKRRSSLS------- 180
           G++A+FLNSLF+Q+ SKK K K K+  K  D   E          R+RRSS+S       
Sbjct: 121 GKIASFLNSLFHQAGSKKNKSKSKSKTKPTDPEVEEEIPGGGWMRRRRRSSISHFFSSSR 180

Query: 181 -------TLIDTKSSSHSSSKISGFRTPPAPNCCTVQTPNKNYMEI-------------- 240
                  T   + S S  SS  SGFRTPP      + TP KNY +               
Sbjct: 181 STSTTTTTTASSSSKSLISSSSSGFRTPPP----YLNTPTKNYKQFLNYTSATKQVGEEE 240

Query: 241 ------RSFLDQKREGINYYSKKNGINNQKSEMR-----KMNDQDEGDESDSSSDLFELR 264
                  S+LD+K + +   S+   I +   ++      K   +D+G ESDSSSDLFEL+
Sbjct: 241 TKTNKEYSWLDEKLKVMESLSENQRIWSDDEDIDDDRRIKREGEDDGMESDSSSDLFELQ 300

BLAST of CSPI05G24420 vs. TAIR 10
Match: AT3G13980.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 1485 Blast hits to 418 proteins in 98 species: Archae - 0; Bacteria - 6; Metazoa - 246; Fungi - 61; Plants - 107; Viruses - 6; Other Eukaryotes - 1059 (source: NCBI BLink). )

HSP 1 Score: 53.5 bits (127), Expect = 3.0e-07
Identity = 65/193 (33.68%), Postives = 82/193 (42.49%), Query Frame = 0

Query: 95  KHRQQPSSPGGRLANFLNSLFNQSAS--KKKKKPKNSIKTEDLHHEMGARKRRS-SLSTL 154
           K  +QP SPGGRLA FLNSLF  +A+  KK KK       E+ H         S S S L
Sbjct: 163 KKVKQPISPGGRLATFLNSLFTNAATNPKKHKKTTTVAVVEEPHSSSTCSSASSFSRSCL 222

Query: 155 IDTKSSSHSSSKI-------------SGFRTPPAPNCCTVQTPN--KNYMEIRSFLDQKR 214
             T SSS  S +              S F  P A N   +   N  K   E R  +   +
Sbjct: 223 SKTPSSSGKSKRSVRFCPVNVILDEDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAK 282

Query: 215 EGINYYSKKNGINNQKSEMRKMNDQDEGDESD----SSSDLFELRICDRFDCYS-SNELP 265
           + +  Y  KN     K     +N+ +E DE D    +SSDLFEL             ELP
Sbjct: 283 DLLRTYHNKN-----KVTTTNINNVEEDDEDDAASCASSDLFELENLSAIGIERYREELP 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z375.8e-2435.28Protein BIG GRAIN 1-like E OS=Arabidopsis thaliana OX=3702 GN=At1g69160 PE=2 SV=... [more]
Q9LVK24.2e-0633.68Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=... [more]
B8APC76.6e-0426.58Protein BIG GRAIN 1 OS=Oryza sativa subsp. indica OX=39946 GN=BG1 PE=3 SV=1[more]
Q10R096.6e-0426.58Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KSX83.4e-14499.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G606790 PE=3 SV=1[more]
A0A5D3CB021.4e-12990.71Protein BIG GRAIN 1-like E OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S4DWX11.4e-12990.71protein BIG GRAIN 1-like E OS=Cucumis melo OX=3656 GN=LOC107990779 PE=3 SV=1[more]
A0A6J1IMQ25.0e-8771.64protein BIG GRAIN 1-like E OS=Cucurbita maxima OX=3661 GN=LOC111478430 PE=3 SV=1[more]
A0A6J1GXU51.6e-8570.97protein BIG GRAIN 1-like E OS=Cucurbita moschata OX=3662 GN=LOC111458485 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_011655719.17.0e-14499.62protein BIG GRAIN 1-like E [Cucumis sativus] >KGN51989.1 hypothetical protein Cs... [more]
XP_016900220.12.9e-12990.71PREDICTED: protein BIG GRAIN 1-like E [Cucumis melo] >KAA0034479.1 protein BIG G... [more]
XP_038892897.19.6e-10978.02protein BIG GRAIN 1-like E [Benincasa hispida][more]
XP_022978456.11.0e-8671.64protein BIG GRAIN 1-like E [Cucurbita maxima][more]
XP_022956957.13.3e-8570.97protein BIG GRAIN 1-like E [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G69160.14.1e-2535.28unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G13980.13.0e-0733.68unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..119
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 215..229
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 127..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 150..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..229
NoneNo IPR availablePANTHERPTHR33541:SF11PROTEIN BIG GRAIN 1-LIKE Ecoord: 208..263
NoneNo IPR availablePANTHERPTHR33541:SF11PROTEIN BIG GRAIN 1-LIKE Ecoord: 1..205
IPR039621Protein BIG GRAIN 1-likePANTHERPTHR33541PROTEIN BIG GRAIN 1-LIKE A-RELATEDcoord: 208..263
coord: 1..205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G24420.1CSPI05G24420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0005886 plasma membrane