CSPI05G23640 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G23640
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Ran guanine nucleotide release factor
Location	Chr5: 23663261 .. 23667290 (-)
RNA-Seq Expression	CSPI05G23640
Synteny	CSPI05G23640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTTCAAATCATTCTTCGTTATCTTCTTCTAATGTTGTAGGAGACTTTTGGGTTTTGGTTAAAATGTCGGCAGATTTCTCTACAGAGCGTGCTCTGTTCGGGGGAGCTATAGTTAGCACATTTCCTCTTAGATTCCAGGTTTGTCTTCTTTAATTGCGATTCTGTCTCTTTCCTTTTTGTTGTATCACTTTTTGTATCTTTATTTAATTCTTTAAATTCATATATTTAATTCTTTATTGTTGTAACTCCAGGATGTGAGCAACGTTCGACAAGTTCCTGATCATCAGGTTGGTAGCCATTTGATTCTTCTATGAAGATAGTTAAGGTTATTTTTTAGTTAAAGCTACTGACTTTGGAAAGTTGCTAATGTTTTTTTTTCCCCTTCTTTTTTGAATATGTTTGTGGCGGATTTATAGGAAGTGTTTGTGGATCCATCTCGGGATGAAAGCTTGATCTTTGAGCTTCTAGATTTTAAGCATGATGTTGGTGACAATGGAAGTGCTGTTTGGTTCCTTCAAGACCTTGCCACTGAGCAGGATGCTGAGGGAAGCGTGGTAAATCTTACTTTTACTTTATGATATACATTTTCTCATCTTTGCTTACTGCCTGCTCTCTCTGCTGTCAGTTTAACATCCAAGTATTAACTAGTAAGCAACAATTTGAGATGGGTTTGCTTGTTTTGTCCATTAAGTACTTAGTTAGAGAGTGTGTGATGTTTTATGGGGGTGATCAGAGATGTTTGAAGAAGTAATTATTTATCTAGTAATATCAGTTTCACTCATCACAAAAGAACTTCCATCTTTCTTCTTTTACAAACTTTGGAAATATGAGAAACAAGACCCTGTGTGTGGATAGTGTAGTCTTCTAAGTTTAATTTCAAGAGATCGATGTCACATTTTATGCAGTCAGATGTTATGGGCTAACTCAAAGCACAGTCATGTTATGTTAGTATTTTGCCTTCTGTTCCAATTAGTTTTTATCTATAAATAAATGTTTGCTTCATTGTTTCATCTTCTTGCAATCCGCACCATCACATTGTCTTATGATATTCTGAACTTGAGCTCTTGATGTAATTTCATGGAATCATGTCGTTTGTTTTACTTATCATGCAATACTTCTTCAGGTGATTGAGCAATCTGGGGTGATCGAGGCACCTGAACTGTGTTATGGAAATATACCTTCTGTAGCTACAACTGCCGTTGGCCAGATGGTATGTGCTGCTGCTGTGTGTGCTGGTTTATCAATTACATAGCTGGTTGGATATTTTAGCTTCAAAACACATCCTTCTTTTCATTTTACTTTATTTAGTAACTTTTTTGTTTCTCGTACCATTTTTGTTTATAATACTAAGTTTACTTCCTCTAGAAAATTTGCATGCTTGCTATTGTTTAACATAGCCTTCAACTCCAACCATTAGCAATCAAATTAGAGATGTCATGTTACTTTGAATTTGTTGTAATTTTAGAAATTTGGCCTTTTTGTATGTCTTGGGTTAGCTACTTCGGATTTAGTTTGTTCTGTTTTTATATGCATTGTGTGAATCACTATATTTAGAACTGTTCAACTGAGACGCATATCTCATGAACAAAATGAAAGACAGAAGTGGAGGCACATGCCCGATGCCTCTCTATCATAATTACAAAGTTAATAATAATAACAGTGCACTAACAAAAGGATGACATTGATTGGTACTACTACTGTTTCACTTAACTGACACAGATCTCTATAGCAAATTCTTTCTTAGTCATGCTCAAAGTGATGCCAGCATTACTAATCAATTTCGTTGTTTCTATTTTTTGTTATATCTGGCATGGTTCTTTGGTTTAAATTTGTTGGTTTGGATGCATCTGCTAAATGTACCAGGCCTGTCAAGACTTTGTTTTTAATTTATTCATGAGAATCATTGGATGTCAGTTGAACTTCGACTTTTCCATGAATTTTTATCCAGTTTTCCTGCTCAGTTGTTGGCAGTTGTTATGGTTGCTAACCGCCATGACTTTGTCACACTTTTTCTTCCCCTTTTTTAAATGGGTCTTGTTTCTGTCCGTTTAGGCAATTTCAAAGGGCCGGCAAGGAAGAGAAGCTCAAAATATTGTCAGGGTAACCTCTCTTATTTTCTTTACCCTAGTGTGGTGGTTCCCAAGGTCTTGTAGGATTAAAATCTGTTTATTGCTTTATGCAGGTTTATTTGGCAAATCTGCGACTTAAGGGAGTTGGTACAGATGTGCTAATCTCTGCATATGAACCCATTGTCATAAAGTGAGTAATTACTCTCAAACAAAAGGAAACAAACAATTAGTAGAATTCTAAGTGAAATAGTTAGTTAAGTCATAGTCTACTTAGATGCATGATATTACTATTGACATCACCACTCCGGCACCTACTCCCTTGACAAACTTATTTAAGGGGCTCACATTCCCCTCACTGCAAAAAATACCCTCCATGATACCACAAATTTGACCTGGTCCTCATGTTTGTTCCTCCTTGTGTTTTTTCCTATCACTGCCCCTTGCCCACACTTTCACTTTGGCCAACCTCAACTGTGAAATATAAAATAGTAGTGTCAGGAACTTCCTGGTGATAGGCATCCTTACCAACTCTCTTAGATATAGGTTTTAGGACCATACTAATGTAAGATGTGTGTTTTCATTCCCCTTATTGCCACTGGCCTCTGTCTGTGAAATATGAATAAATATACAAGTTCATCGATTGTTTTACATTGCTAGCTCTCTTCATGCAGTCTACTTGAATGAGTAATTCATATTTATTCATATCACTCCAGATGTACACTCCCGTAATAGGGTCCACCGTCCTCACTACAATGATCTACCAGGCGGATAACTAATTTGAAAGCTAAATAACTAATTATTACCCTCTGTTTTCCTTCTTGTACATGAAGCATATGGAGAATCTTACTTGTTCAGTTTGTATTTTTTTGCGTGTTCTTAATTTGAAATTTAGGATTAGTTACCATCTGTCATATTGCCAATAATCATAACTCAAGCTGTTTTGCTTTACATCGTAGAATAATGCATACAACGCTTTCTTCTTTACAATGTTGATTGGTTTCCTTTACACCAGGGCTAACAGCCAGATTGAACTGTTATGGTCTAGTATAAGAAAACATGATACTGAGAAACTCAAGAACGTAGAGATGCATCCCGAAAGGATGGCACTATCTCAATCATTTTGTAGGGTGATAAGGGCCCAAAACAGAACCATCTATTTTAATTGGCCAACTTGGCTAAGTTACATTTTTATATCTGGCCCAGTCTGAAGCAAAGCCCATTTTCTTACGTCTTCATAAATTACAACCCAAATAGGATTCAAACGTGCAACTAGCATAGCATATCTTTCTTCTTGTACCTGTCACTTGCAACGCAACACTATTGCTTATAATTGTGCTACATTGCTGTGGTTGCAGTCCTTTGAGCGAAAGTGCTAGCTCTGTCGGCTCTGGATTGGCCACTCCTGCCTCACACTCAGGTTACATGCCAATGCCTGAGGTATTCAAGCTTGCAGTCTCTAGCTTTAAAGTCTGTGACTGGAGCCTTTTTGGTGCGCCTGCCTAATCTAGGAGATAGGATCAGCGTTTGTTCATTATTATTTCATTAGTATGTTATGTTACATTGGGAATGTTCCCTGCTGCTCTCTTGAGTTAGAAGTCGTTTGTAGAGGTTTTAGAGCTGTTCGTTGTTGTTGGGTGTTGATTTTTAATCAACGGAAAAGTTGAAAATATGAAGTAATGGCATATGTAAATGGTAGAAGTGCCCTTTAAGGTGGACAAAGTAGAACAAGTATTAGCTGTTTGTAGTAGGACCCCATATTTTTGCATCAGTCAATCACGTGGCAAACAAAAGGTTTGAATATTAAACCGTGACTGACTGGCCATATTATGTAGTCTAGTTTGTGTTTTCACACATGCAATGGTGACCTTTTCTTGGGGGGAAAAAAAGAAAGAAAGAAATTATTGGTGGGCGTTTTACATCAAATTATTGTCTAATCTTCTAATAATTGCGGAAGGCACAA

mRNA sequence

GTTTTCAAATCATTCTTCGTTATCTTCTTCTAATGTTGTAGGAGACTTTTGGGTTTTGGTTAAAATGTCGGCAGATTTCTCTACAGAGCGTGCTCTGTTCGGGGGAGCTATAGTTAGCACATTTCCTCTTAGATTCCAGGATGTGAGCAACGTTCGACAAGTTCCTGATCATCAGGAAGTGTTTGTGGATCCATCTCGGGATGAAAGCTTGATCTTTGAGCTTCTAGATTTTAAGCATGATGTTGGTGACAATGGAAGTGCTGTTTGGTTCCTTCAAGACCTTGCCACTGAGCAGGATGCTGAGGGAAGCGTGGTGATTGAGCAATCTGGGGTGATCGAGGCACCTGAACTGTGTTATGGAAATATACCTTCTGTAGCTACAACTGCCGTTGGCCAGATGGCAATTTCAAAGGGCCGGCAAGGAAGAGAAGCTCAAAATATTGTCAGGGTTTATTTGGCAAATCTGCGACTTAAGGGAGTTGGTACAGATGTGCTAATCTCTGCATATGAACCCATTGTCATAAATCCTTTGAGCGAAAGTGCTAGCTCTGTCGGCTCTGGATTGGCCACTCCTGCCTCACACTCAGGTTACATGCCAATGCCTGAGGTATTCAAGCTTGCAGTCTCTAGCTTTAAAGTCTGTGACTGGAGCCTTTTTGGTGCGCCTGCCTAATCTAGGAGATAGGATCAGCGTTTGTTCATTATTATTTCATTAGTATGTTATGTTACATTGGGAATGTTCCCTGCTGCTCTCTTGAGTTAGAAGTCGTTTGTAGAGGTTTTAGAGCTGTTCGTTGTTGTTGGGTGTTGATTTTTAATCAACGGAAAAGTTGAAAATATGAAGTAATGGCATATGTAAATGGTAGAAGTGCCCTTTAAGGTGGACAAAGTAGAACAAGTATTAGCTGTTTGTAGTAGGACCCCATATTTTTGCATCAGTCAATCACGTGGCAAACAAAAGGTTTGAATATTAAACCGTGACTGACTGGCCATATTATGTAGTCTAGTTTGTGTTTTCACACATGCAATGGTGACCTTTTCTTGGGGGGAAAAAAAGAAAGAAAGAAATTATTGGTGGGCGTTTTACATCAAATTATTGTCTAATCTTCTAATAATTGCGGAAGGCACAA

Coding sequence (CDS)

ATGTCGGCAGATTTCTCTACAGAGCGTGCTCTGTTCGGGGGAGCTATAGTTAGCACATTTCCTCTTAGATTCCAGGATGTGAGCAACGTTCGACAAGTTCCTGATCATCAGGAAGTGTTTGTGGATCCATCTCGGGATGAAAGCTTGATCTTTGAGCTTCTAGATTTTAAGCATGATGTTGGTGACAATGGAAGTGCTGTTTGGTTCCTTCAAGACCTTGCCACTGAGCAGGATGCTGAGGGAAGCGTGGTGATTGAGCAATCTGGGGTGATCGAGGCACCTGAACTGTGTTATGGAAATATACCTTCTGTAGCTACAACTGCCGTTGGCCAGATGGCAATTTCAAAGGGCCGGCAAGGAAGAGAAGCTCAAAATATTGTCAGGGTTTATTTGGCAAATCTGCGACTTAAGGGAGTTGGTACAGATGTGCTAATCTCTGCATATGAACCCATTGTCATAAATCCTTTGAGCGAAAGTGCTAGCTCTGTCGGCTCTGGATTGGCCACTCCTGCCTCACACTCAGGTTACATGCCAATGCCTGAGGTATTCAAGCTTGCAGTCTCTAGCTTTAAAGTCTGTGACTGGAGCCTTTTTGGTGCGCCTGCCTAA

Protein sequence

MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDVGDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQGREAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMPEVFKLAVSSFKVCDWSLFGAPA*

Homology

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BLAST of CSPI05G23640 vs. ExPASy Swiss-Prot
Match: Q54ML6 (Probable ran guanine nucleotide release factor OS=Dictyostelium discoideum OX=44689 GN=mog1 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.4e-17
Identity = 64/203 (31.53%), Postives = 109/203 (53.69%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60
           MS  F  +R L+GGAI    P RF DV++ R +PDHQE+F D   D+S+I EL +F+  +
Sbjct: 1   MSETFE-KRQLYGGAIEIDIPRRFIDVTSYRHIPDHQELFSDEKSDQSVIIELNEFQDHI 60

Query: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATT-----AVGQMAIS 120
             N +A+    ++  E   +  +  ++S ++   EL    +P+   +      + Q  I+
Sbjct: 61  -SNANAIKHHYEVLVE---DAGISTDKSVILNFRELTQAEMPNFDASIPKYVLLAQQKIA 120

Query: 121 KGRQGREAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSG 180
           K  +   A+N + +Y+A +RL+   TD+LI+  E I + P S S + V +   TP++   
Sbjct: 121 KFNE--TAENTINIYMALVRLEKSKTDLLITFNEAIALAPTSSSVAVVQN--LTPSNDQS 180

Query: 181 YMPMPEVFKLAVSSFKVCDWSLF 199
                ++F   + SFK+ D+SLF
Sbjct: 181 --KSEQLFLTMLKSFKIKDYSLF 192

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BLAST of CSPI05G23640 vs. ExPASy Swiss-Prot
Match: A3KPP3 (Ran guanine nucleotide release factor OS=Danio rerio OX=7955 GN=rangrf PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 8.9e-17
Identity = 57/155 (36.77%), Postives = 85/155 (54.84%), Query Frame = 0


Query: 9   RALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDVGDNGSAVW 68
           R LFGGA+ + FP    D+S +RQ+PD+QEVF     D+S+I ELL+++  V D  +A +
Sbjct: 3   RPLFGGALSAVFPSSVMDISELRQIPDNQEVFAHSQTDQSIIIELLEYQSQVQDADAARY 62

Query: 69  FLQDLATEQDAEGSVVIEQSGVIEAP--ELCYGNIPSVATTAVGQMAISKGRQGREAQNI 128
             +D+A    A  +   E   V + P  E+      S A    G   +SK  +  EA+N 
Sbjct: 63  HFEDVAGSNKAIENGTWEVRVVEQVPQSEISMQECSS-AWLLSGAQLVSKFNE--EAKNT 122

Query: 129 VRVYLANLRLKGVGTDVLISAYEPIVINPLSESAS 162
           V V+    RL    TD+L++  +P+ INPLS SA+
Sbjct: 123 VNVHQCLFRLPQFTTDILMTFNDPVFINPLSSSAA 154

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BLAST of CSPI05G23640 vs. ExPASy Swiss-Prot
Match: Q32PE2 (Ran guanine nucleotide release factor OS=Bos taurus OX=9913 GN=RANGRF PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.3e-11
Identity = 60/193 (31.09%), Postives = 95/193 (49.22%), Query Frame = 0


Query: 8   ERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDVGDNGSAV 67
           +  LFGGA  +T P    DVS++R VPDHQEVF     D+SLI ELL+ +  V    +A 
Sbjct: 6   DNPLFGGAFSATLPPGAIDVSDLRPVPDHQEVFCHRVTDQSLIVELLELQAHVQGEEAAR 65

Query: 68  WFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQGREAQNIV 127
           +  +D+   Q+A  +V +E    +   +L        A    GQ  ++K  + ++    V
Sbjct: 66  YHFEDVGGVQEAR-AVQVETVQPLVLEKLALRGCCQEAWILSGQQQVAK--ENQQVAKYV 125

Query: 128 RVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVG-SGLATPASHSGYMPMPEVFKLA 187
            ++ A LRL    TD+L++  +     P  E+ SS+G   L+ P    G       F+  
Sbjct: 126 TLHQALLRLPQYQTDLLLTFNQ-----PPPENRSSLGPENLSIPPWSLG------DFEQL 184

Query: 188 VSSFKVCDWSLFG 200
           V+S  + D ++FG
Sbjct: 186 VTSLTLHDPNIFG 184

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BLAST of CSPI05G23640 vs. ExPASy Swiss-Prot
Match: Q9HD47 (Ran guanine nucleotide release factor OS=Homo sapiens OX=9606 GN=RANGRF PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.2e-10
Identity = 58/189 (30.69%), Postives = 93/189 (49.21%), Query Frame = 0


Query: 11  LFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDVGDNGSAVWFL 70
           LFGGA  +  P+   DVS++R VPD+QEVF  P  D+SLI ELL+ +  V    +A +  
Sbjct: 9   LFGGAFSAILPMGAIDVSDLRPVPDNQEVFCHPVTDQSLIVELLELQAHVRGEAAARYHF 68

Query: 71  QDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQGREAQNIVRVY 130
           +D+   Q A  +V +E    +    L        A    G+  I+K  + ++    V ++
Sbjct: 69  EDVGGVQGAR-AVHVESVQPLSLENLALRGRCQEAWVLSGKQQIAK--ENQQVAKDVTLH 128

Query: 131 LANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMPEVFKLAVSSF 190
            A LRL    TD+L++  +     P  ++ SS+G    +PA  S        F+  V+S 
Sbjct: 129 QALLRLPQYQTDLLLTFNQ-----PPPDNRSSLGPENLSPAPWS-----LGDFEQLVTSL 184

Query: 191 KVCDWSLFG 200
            + D ++FG
Sbjct: 189 TLHDPNIFG 184

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BLAST of CSPI05G23640 vs. ExPASy Swiss-Prot
Match: O75002 (Nuclear import protein mog1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mog1 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 8.1e-10
Identity = 53/192 (27.60%), Postives = 90/192 (46.88%), Query Frame = 0


Query: 11  LFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDE-SLIFELLDFKHDVGDNGSAVWF 70
           LFGGA+ + FP +F D S +RQ+PD+QEVF+  S++  ++I ELL+      D   A + 
Sbjct: 4   LFGGALCADFPPKFLDASVLRQIPDNQEVFLQDSKENLTVIIELLEKIEKPFDGSVAAYH 63

Query: 71  LQDLATEQDAEGSVVIEQSGVIEAP-ELCYGNIPSVATTAVGQMAISKGRQGREAQNIVR 130
              +A + DA   V+     + E   E       S ++    Q  + KG++  E+   V 
Sbjct: 64  FNSIAFDNDASQRVIWRDKSLGEDDFEGMRSEKASGSSVQGCQRVLEKGKRNPESATNVA 123

Query: 131 VYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLAT--PASHSGYMPMPEVFKLA 190
           +++  + L    TD++IS   P+       + SSV S +    P+  S      E  +  
Sbjct: 124 IFVNVITLIDFQTDIVISVNAPL------PNTSSVPSSVENIPPSDQSIVRAALETIQRV 183

Query: 191 VSSFKVCDWSLF 199
             S  + D ++F
Sbjct: 184 TRSLVLVDKTVF 189

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BLAST of CSPI05G23640 vs. ExPASy TrEMBL
Match: A0A0A0KU01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G605010 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 8.7e-108
Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60
           MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV
Sbjct: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60

Query: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120
           GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG
Sbjct: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120

Query: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180
           REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP
Sbjct: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180

Query: 181 EVFKLAVSSFKVCDWSLFGAPA 203
           EVFKLAVSSFKVCDWSLFGAPA
Sbjct: 181 EVFKLAVSSFKVCDWSLFGAPA 202

[Close]

BLAST of CSPI05G23640 vs. ExPASy TrEMBL
Match: A0A1S3BEU3 (probable ran guanine nucleotide release factor OS=Cucumis melo OX=3656 GN=LOC103489108 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 9.0e-105
Identity = 196/202 (97.03%), Postives = 199/202 (98.51%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60
           MSADFSTER LFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV
Sbjct: 1   MSADFSTERPLFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60

Query: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120
           GDNGSAVWFLQDLATEQDAEGS+VIEQSGVIEAP LCYGNIP+VATTAVGQMAISKGRQG
Sbjct: 61  GDNGSAVWFLQDLATEQDAEGSMVIEQSGVIEAPGLCYGNIPTVATTAVGQMAISKGRQG 120

Query: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180
           REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGL TPASHSGY+PMP
Sbjct: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLTTPASHSGYIPMP 180

Query: 181 EVFKLAVSSFKVCDWSLFGAPA 203
           EVFKLAVSSFKVCDWSLFGAPA
Sbjct: 181 EVFKLAVSSFKVCDWSLFGAPA 202

[Close]

BLAST of CSPI05G23640 vs. ExPASy TrEMBL
Match: A0A5A7SUT7 (Putative ran guanine nucleotide release factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00530 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.0e-99
Identity = 192/212 (90.57%), Postives = 195/212 (91.98%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQ--------------DVSNVRQVPDHQEVFVDPSRD 60
           MSADFSTER LFGGAIVSTFPLRFQ              DVSNVRQVPDHQEVFVDPSRD
Sbjct: 1   MSADFSTERPLFGGAIVSTFPLRFQVVFFNSDSVSFPFVDVSNVRQVPDHQEVFVDPSRD 60

Query: 61  ESLIFELLDFKHDVGDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVAT 120
           ESLIFELLDFKHDVGDNGSAVWFLQDLATEQDAEGS+VIEQSGVIEAP LCYGNIP+VAT
Sbjct: 61  ESLIFELLDFKHDVGDNGSAVWFLQDLATEQDAEGSMVIEQSGVIEAPGLCYGNIPTVAT 120

Query: 121 TAVGQMAISKGRQGREAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSG 180
           TAVGQMAISKGRQGREAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSG
Sbjct: 121 TAVGQMAISKGRQGREAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSG 180

Query: 181 LATPASHSGYMPMPEVFKLAVSSFKVCDWSLF 199
           L TPASHSGY+PMPEVFKLAVSSFKVCDWSLF
Sbjct: 181 LTTPASHSGYIPMPEVFKLAVSSFKVCDWSLF 212

[Close]

BLAST of CSPI05G23640 vs. ExPASy TrEMBL
Match: A0A6J1JJW2 (probable ran guanine nucleotide release factor OS=Cucurbita maxima OX=3661 GN=LOC111485103 PE=3 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 2.4e-97
Identity = 184/200 (92.00%), Postives = 191/200 (95.50%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60
           M+ DFS+ERALFGGAIVSTFPLRFQDVSN+RQVPDHQEVFVDPSRDESLIFELLDFKHDV
Sbjct: 1   MAEDFSSERALFGGAIVSTFPLRFQDVSNIRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60

Query: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120
            DNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAP LCY NIPSV TTAVGQMAISKGRQG
Sbjct: 61  EDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPGLCYANIPSVVTTAVGQMAISKGRQG 120

Query: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180
           REAQNIVRVYLANLRLK VGTDVLI AYEPI+INPLSESASSVGSGLATPAS SG +PMP
Sbjct: 121 REAQNIVRVYLANLRLKAVGTDVLICAYEPILINPLSESASSVGSGLATPASQSGCIPMP 180

Query: 181 EVFKLAVSSFKVCDWSLFGA 201
           EVFKLA+SSFKVCDWS+FGA
Sbjct: 181 EVFKLALSSFKVCDWSVFGA 200

[Close]

BLAST of CSPI05G23640 vs. ExPASy TrEMBL
Match: A0A6J1GZ86 (probable ran guanine nucleotide release factor OS=Cucurbita moschata OX=3662 GN=LOC111458863 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.2e-96
Identity = 182/200 (91.00%), Postives = 189/200 (94.50%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60
           M+ DFS+ERALFGGAIVSTFP+RFQDVSN+RQVPDHQEVFVDPSRDESLIFELLDFKHDV
Sbjct: 1   MAEDFSSERALFGGAIVSTFPIRFQDVSNIRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60

Query: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120
            DNGSAVWFLQDLATEQDAEGSVVIEQSGVIE P LCY NIPSV TTAVGQMAISKGRQG
Sbjct: 61  EDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEVPGLCYANIPSVVTTAVGQMAISKGRQG 120

Query: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180
           REAQNIVRVYLANLRLK VGTDVLI AYEPI+INPLSESASSVGSGL TPAS SG MPMP
Sbjct: 121 REAQNIVRVYLANLRLKAVGTDVLICAYEPILINPLSESASSVGSGLTTPASQSGCMPMP 180

Query: 181 EVFKLAVSSFKVCDWSLFGA 201
           EVFKLA+SSFKVCDWS+FGA
Sbjct: 181 EVFKLALSSFKVCDWSVFGA 200

[Close]

BLAST of CSPI05G23640 vs. NCBI nr
Match: XP_004135179.1 (ran guanine nucleotide release factor [Cucumis sativus] >KGN51907.1 hypothetical protein Csa_008794 [Cucumis sativus])

HSP 1 Score: 399.4 bits (1025), Expect = 1.8e-107
Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60
           MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV
Sbjct: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60

Query: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120
           GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG
Sbjct: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120

Query: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180
           REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP
Sbjct: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180

Query: 181 EVFKLAVSSFKVCDWSLFGAPA 203
           EVFKLAVSSFKVCDWSLFGAPA
Sbjct: 181 EVFKLAVSSFKVCDWSLFGAPA 202

[Close]

BLAST of CSPI05G23640 vs. NCBI nr
Match: XP_008446339.1 (PREDICTED: probable ran guanine nucleotide release factor [Cucumis melo])

HSP 1 Score: 389.4 bits (999), Expect = 1.9e-104
Identity = 196/202 (97.03%), Postives = 199/202 (98.51%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60
           MSADFSTER LFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV
Sbjct: 1   MSADFSTERPLFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60

Query: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120
           GDNGSAVWFLQDLATEQDAEGS+VIEQSGVIEAP LCYGNIP+VATTAVGQMAISKGRQG
Sbjct: 61  GDNGSAVWFLQDLATEQDAEGSMVIEQSGVIEAPGLCYGNIPTVATTAVGQMAISKGRQG 120

Query: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180
           REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGL TPASHSGY+PMP
Sbjct: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLTTPASHSGYIPMP 180

Query: 181 EVFKLAVSSFKVCDWSLFGAPA 203
           EVFKLAVSSFKVCDWSLFGAPA
Sbjct: 181 EVFKLAVSSFKVCDWSLFGAPA 202

[Close]

BLAST of CSPI05G23640 vs. NCBI nr
Match: KAA0034408.1 (putative ran guanine nucleotide release factor [Cucumis melo var. makuwa] >TYK17724.1 putative ran guanine nucleotide release factor [Cucumis melo var. makuwa])

HSP 1 Score: 371.7 bits (953), Expect = 4.0e-99
Identity = 192/212 (90.57%), Postives = 195/212 (91.98%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQ--------------DVSNVRQVPDHQEVFVDPSRD 60
           MSADFSTER LFGGAIVSTFPLRFQ              DVSNVRQVPDHQEVFVDPSRD
Sbjct: 1   MSADFSTERPLFGGAIVSTFPLRFQVVFFNSDSVSFPFVDVSNVRQVPDHQEVFVDPSRD 60

Query: 61  ESLIFELLDFKHDVGDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVAT 120
           ESLIFELLDFKHDVGDNGSAVWFLQDLATEQDAEGS+VIEQSGVIEAP LCYGNIP+VAT
Sbjct: 61  ESLIFELLDFKHDVGDNGSAVWFLQDLATEQDAEGSMVIEQSGVIEAPGLCYGNIPTVAT 120

Query: 121 TAVGQMAISKGRQGREAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSG 180
           TAVGQMAISKGRQGREAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSG
Sbjct: 121 TAVGQMAISKGRQGREAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSG 180

Query: 181 LATPASHSGYMPMPEVFKLAVSSFKVCDWSLF 199
           L TPASHSGY+PMPEVFKLAVSSFKVCDWSLF
Sbjct: 181 LTTPASHSGYIPMPEVFKLAVSSFKVCDWSLF 212

[Close]

BLAST of CSPI05G23640 vs. NCBI nr
Match: XP_023552843.1 (probable ran guanine nucleotide release factor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 365.9 bits (938), Expect = 2.2e-97
Identity = 184/200 (92.00%), Postives = 191/200 (95.50%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60
           M+ DFS+ERALFGGAIVSTFP+RFQDVSN+RQVPDHQEVFVDPSRDESLIFELLDFKHDV
Sbjct: 1   MAEDFSSERALFGGAIVSTFPIRFQDVSNIRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60

Query: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120
            DNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAP LCY NIPSV TTAVGQMAISKGRQG
Sbjct: 61  EDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPGLCYANIPSVVTTAVGQMAISKGRQG 120

Query: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180
           REAQNIVRVYLANLRLK VGTDVLI AYEPI+INPLSESASSVGSGLATPAS SG MPMP
Sbjct: 121 REAQNIVRVYLANLRLKAVGTDVLICAYEPILINPLSESASSVGSGLATPASQSGCMPMP 180

Query: 181 EVFKLAVSSFKVCDWSLFGA 201
           EVFKLA+SSFKVCDWS+FGA
Sbjct: 181 EVFKLALSSFKVCDWSIFGA 200

[Close]

BLAST of CSPI05G23640 vs. NCBI nr
Match: XP_022987588.1 (probable ran guanine nucleotide release factor [Cucurbita maxima])

HSP 1 Score: 364.8 bits (935), Expect = 4.9e-97
Identity = 184/200 (92.00%), Postives = 191/200 (95.50%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60
           M+ DFS+ERALFGGAIVSTFPLRFQDVSN+RQVPDHQEVFVDPSRDESLIFELLDFKHDV
Sbjct: 1   MAEDFSSERALFGGAIVSTFPLRFQDVSNIRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60

Query: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120
            DNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAP LCY NIPSV TTAVGQMAISKGRQG
Sbjct: 61  EDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPGLCYANIPSVVTTAVGQMAISKGRQG 120

Query: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180
           REAQNIVRVYLANLRLK VGTDVLI AYEPI+INPLSESASSVGSGLATPAS SG +PMP
Sbjct: 121 REAQNIVRVYLANLRLKAVGTDVLICAYEPILINPLSESASSVGSGLATPASQSGCIPMP 180

Query: 181 EVFKLAVSSFKVCDWSLFGA 201
           EVFKLA+SSFKVCDWS+FGA
Sbjct: 181 EVFKLALSSFKVCDWSVFGA 200

[Close]

BLAST of CSPI05G23640 vs. TAIR 10
Match: AT1G69680.1 (Mog1/PsbP/DUF1795-like photosystem II reaction center PsbP family protein )

HSP 1 Score: 294.7 bits (753), Expect = 5.8e-80
Identity = 148/202 (73.27%), Postives = 173/202 (85.64%), Query Frame = 0


Query: 1   MSADFSTERALFGGAIVSTFPLRFQDVSNVRQVPDHQEVFVDPSRDESLIFELLDFKHDV 60
           MS +  + R LFGGAI + FP RFQDVSN+RQVPDHQEVFVDPSRDESLIFELLDFK +V
Sbjct: 1   MSVELCSVRPLFGGAISTVFPQRFQDVSNIRQVPDHQEVFVDPSRDESLIFELLDFKAEV 60

Query: 61  GDNGSAVWFLQDLATEQDAEGSVVIEQSGVIEAPELCYGNIPSVATTAVGQMAISKGRQG 120
           GD GSA WFL DLA+EQDAEG  +IEQS VIEAP L + NI +VATTA+G+MAISKGRQG
Sbjct: 61  GDIGSASWFLNDLASEQDAEGFQLIEQSEVIEAPGLSFRNISAVATTAIGEMAISKGRQG 120

Query: 121 REAQNIVRVYLANLRLKGVGTDVLISAYEPIVINPLSESASSVGSGLATPASHSGYMPMP 180
           REAQN+VRVY+AN+RLKGV TDVL++AYEPI+INPLSESA +VGSGLA PAS SG MPM 
Sbjct: 121 REAQNLVRVYVANIRLKGVDTDVLVTAYEPILINPLSESADAVGSGLAVPASQSGKMPMC 180

Query: 181 EVFKLAVSSFKVCDWSLFGAPA 203
           ++ K ++S+FKV DW+LFG+ A
Sbjct: 181 DIIKQSLSTFKVNDWNLFGSSA 202

The following BLAST results are available for this feature:

BLAST of CSPI05G23640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54ML6	2.4e-17	31.53	Probable ran guanine nucleotide release factor OS=Dictyostelium discoideum OX=44...	[more]
A3KPP3	8.9e-17	36.77	Ran guanine nucleotide release factor OS=Danio rerio OX=7955 GN=rangrf PE=2 SV=1	[more]
Q32PE2	3.3e-11	31.09	Ran guanine nucleotide release factor OS=Bos taurus OX=9913 GN=RANGRF PE=2 SV=1	[more]
Q9HD47	1.2e-10	30.69	Ran guanine nucleotide release factor OS=Homo sapiens OX=9606 GN=RANGRF PE=1 SV=...	[more]
O75002	8.1e-10	27.60	Nuclear import protein mog1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484...	[more]

BLAST of CSPI05G23640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KU01	8.7e-108	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G605010 PE=3 SV=1	[more]
A0A1S3BEU3	9.0e-105	97.03	probable ran guanine nucleotide release factor OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A5A7SUT7	2.0e-99	90.57	Putative ran guanine nucleotide release factor OS=Cucumis melo var. makuwa OX=11...	[more]
A0A6J1JJW2	2.4e-97	92.00	probable ran guanine nucleotide release factor OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GZ86	1.2e-96	91.00	probable ran guanine nucleotide release factor OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of CSPI05G23640 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135179.1	1.8e-107	100.00	ran guanine nucleotide release factor [Cucumis sativus] >KGN51907.1 hypothetical...	[more]
XP_008446339.1	1.9e-104	97.03	PREDICTED: probable ran guanine nucleotide release factor [Cucumis melo]	[more]
KAA0034408.1	4.0e-99	90.57	putative ran guanine nucleotide release factor [Cucumis melo var. makuwa] >TYK17...	[more]
XP_023552843.1	2.2e-97	92.00	probable ran guanine nucleotide release factor [Cucurbita pepo subsp. pepo]	[more]
XP_022987588.1	4.9e-97	92.00	probable ran guanine nucleotide release factor [Cucurbita maxima]	[more]

BLAST of CSPI05G23640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69680.1	5.8e-80	73.27	Mog1/PsbP/DUF1795-like photosystem II reaction center PsbP family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007681	Ran-interacting Mog1 protein	PFAM	PF04603	Mog1	coord: 10..150 e-value: 3.9E-36 score: 124.4
IPR007681	Ran-interacting Mog1 protein	PANTHER	PTHR15837	RAN GUANINE NUCLEOTIDE RELEASE FACTOR	coord: 2..153
None	No IPR available	GENE3D	3.40.1000.10	Mog1/PsbP, alpha/beta/alpha sandwich	coord: 5..154 e-value: 3.7E-38 score: 133.2
None	No IPR available	PANTHER	PTHR15837:SF4	BNAA07G24140D PROTEIN	coord: 2..153
IPR016123	Mog1/PsbP, alpha/beta/alpha sandwich	SUPERFAMILY	55724	Mog1p/PsbP-like	coord: 7..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G23640.1	CSPI05G23640.1	mRNA
CSPI05G23640.2	CSPI05G23640.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006606	protein import into nucleus
biological_process	GO:0050790	regulation of catalytic activity
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005085	guanyl-nucleotide exchange factor activity
molecular_function	GO:0031267	small GTPase binding