CSPI05G20330 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G20330
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	calumenin-B
Location	Chr5: 21441661 .. 21444579 (+)
RNA-Seq Expression	CSPI05G20330
Synteny	CSPI05G20330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAAATTCTCAGTTCTCATTTACATTTCAATCTTTTCCCTTCTTCTAATCCTAATATCTCAAACTCCAACAACCCGCCCCCGTCACCGCCGCCTCCGCCTCCGCTCCAACTTCACCTTCAATCCCCCCCTCCTCCGCCACCAACACGACCACTACATCTCCTTCGACCCTCTCATTTCTGACATTGAGCTCCGCCGTGAGGACGACGAATGGCATAAGCAGTCCCTCAAAAATCTCGCCGCCCATGATTCTCATCCTGAGTGGGAAGAGTTCATCAACGATGAGGACAGGTTTAATGTCACCGAAAGATTGTTTTGGATTTTTCCCAAAATTGATGTGGATCCTTCTGATGGGTTTGTGTCGGCGGAGGAACTGACTCGCTGGAACTTGCAGCAAGCCATGAATGAAGCTTTGTATCGGACTGAAAGGGAATTCCAATCTCATGATGATAATCGTGATGGGTTTGTTTCCTTTGCTGAGTATGAACCCCCCAGTTGGGTTCTTTCTGCCGGTAAATCTTCCTCTTCTTTCTAATCACTTGTCAATTTGATTTCACTTTGATTTTAAGAGCCCCTGCTGATTAATGTGTTCTTGTTTGGTGAATTTGTGTTTTAGTATCTTTTGTACTTTAGACTATTCTATCTGATATGACACGTTTGTTTCCCAATGGCATTTATGATTTCTTTTTGTTTAATACAAGTGGGTAGTGGCAAGTGGGTGGGGACAAACACCTTTGAATTTATCGAGGGTATATGGTTTAATAAGCTGGACCTTGCGGAGGTTGTAATATTAACTTATTAATGCTTTACTTTACCAGTCTTTGAAGCAGGAAGTTACCTTTTTAGCTAAGACAAATATGGTTAATGTTTTTGTATATGGTTCTATGACATAATTTATGAATTCTCCAATAGGATTCCTAAAAGAGTTTTTGGCTCATAAAGCTTCAACCTAAGTTTGTGTCATTGGTGGTTTATGTGTTTAATATGGTTGGGTTTTGGTTTTTCTTTTTTGAGATAGTGTAATAAAATTTGTTGAATTTCTGGGTTGATGAGTTATCTTGTGTTTTTATGTGGGTTCATGACTTTTTGTATTAAGAAAGTACATACCCTGTATTTGATTTCTGAGTTTACGAAGCCTAAAACTTTACCATATGATATGAAGATAGGATTCATAAGCTTGAATTTTCGTTACTTCGATTATGTTAACTTTAAGAATATAACTCTTTCATAGTCTAAAACAAGCAATCCATAGTTTTACATAGGCTTGCTTTGATAAACACCCTTAAATTGCTTCTTCTTCTCATAGAAGATTTTTAAAGATTCCAACGGTGTTGTTTATGTTACAGATTCTGCTGTTTAATTCCCAACTGTTTATCAGGTAATGGTTCTTTTGGCTATGATATTGGCTGGTGGAATGAGGAGCACTTCAATGCATCAGATGCAGATGGGGATGGAGTTTTGGATCTAAATGAATTTAATGAGTAAGCTTCAAAAGTTTCACCATCTCTCTTTTGTCCCAACCTCTCACATCATCTGAAAATGCTAAGAAAAAGTTTTGTCATTTTTTATCACTCAATTGTCAATACTTATACCATTGTACGATCTACTTGTTTTCAGCTTCTTGCATCCTGCTGACAGCAAGAGCACGAAGCTGCTTCTATGGTTATGTGCAGATGTAGTAAGGTAAATGGATTCCTTAAGCCTTCGGAGCTTATTACCGAGATAGTTAAACATAACGAGCACTTTACATGAACTTAAATGTACAAAGATTCTAACACTTGTTCCAATCTTCTTTGTGTTGAAACCTTCTGTTGTATTTAAAGTTAGTACTGATAATCTTACAAGATCATTCTACTGACCACTCACTTTATAATGTTACCTATCAGGGAAAGAGATAACGACAAAGATGGAAAGCTCAATTTCAGTGAGTTTTTCCCTAAAGTTTTAGACTTAGTAAGAAGAGTGGATGAAGACTACAATTCATCAAATTGGGAGGACGAAGAACCGGAGGCACTGGCAAAAAGGATGTTCTTGGAGCTCGACAAAGACTCTGATGGGTAAAAGTAAAACATTATTCCCATATATGTCTTTCTGTTGTAAATAATTCACTCTCTAAACATTATATTGTTCTTCACCCTTACAGGTACCTTTCAACAACTGAAATGTTGCCTATAATTGGAAAAATCCATCCATCGGAGGCTTATTATGCAAAACAACAAGCAGAGTACATCATCTCTCAGGTACTTTTGGATGATAAAACATGGTTTCCGCTTTTATTGTTGCATCGTAAATGTCTTACAACCCAAAGGGGTTAAACAATTCACAAAGTTTTCAACCGAAAAAATGTTCTATATTTCTGGTAATAAGAAAGTATTCTGCGGGATTACTGTGAACTTGTTATAGGAAGTATTAATCTTCATGTTCTAGATTCTCAGGATCACACGATGAATGATTTCCATAGTTTTGAGGGCCTTAAACAGATAATTGATGTCATGAATTGCAGGCAGATAGTGATGATGATGGACTCCTAACCTTGAACGATATGATCGAGAACCCATCTGTGTTCTATAGCTCTGTTTTTACTGAAGACGAGATGGATTATTATCCATACCACGATGAATTTCGTTAGATGGATACAGTAAGAACAACTACGACATTTACTTGTAATCTATATTTCTTTCATCCTCTTGATATCTTTTTCTTAATTCACTATTCACAATTTCTTTCTTTGCTTGCAGGTTATTTAAAGAAAAAAAAGATTATGGGAGAAAAGAAAACGACGTTGAGAAAGCTTGAGAATTATCAAGAGATTGATCTTCACAAAATATTAGTTAGAGGTGATTTGCTTTCTTGTTTGTAGCTTAATTAGTTTTATTCTGGCCCTAGCATAGTGTCGGTTTTAATTTCTTGAGTCAGG

mRNA sequence

ATGGGGAAATTCTCAGTTCTCATTTACATTTCAATCTTTTCCCTTCTTCTAATCCTAATATCTCAAACTCCAACAACCCGCCCCCGTCACCGCCGCCTCCGCCTCCGCTCCAACTTCACCTTCAATCCCCCCCTCCTCCGCCACCAACACGACCACTACATCTCCTTCGACCCTCTCATTTCTGACATTGAGCTCCGCCGTGAGGACGACGAATGGCATAAGCAGTCCCTCAAAAATCTCGCCGCCCATGATTCTCATCCTGAGTGGGAAGAGTTCATCAACGATGAGGACAGGTTTAATGTCACCGAAAGATTGTTTTGGATTTTTCCCAAAATTGATGTGGATCCTTCTGATGGGTTTGTGTCGGCGGAGGAACTGACTCGCTGGAACTTGCAGCAAGCCATGAATGAAGCTTTGTATCGGACTGAAAGGGAATTCCAATCTCATGATGATAATCGTGATGGGTTTGTTTCCTTTGCTGAGTATGAACCCCCCAGTTGGGTTCTTTCTGCCGGTAATGGTTCTTTTGGCTATGATATTGGCTGGTGGAATGAGGAGCACTTCAATGCATCAGATGCAGATGGGGATGGAGTTTTGGATCTAAATGAATTTAATGACTTCTTGCATCCTGCTGACAGCAAGAGCACGAAGCTGCTTCTATGGTTATGTGCAGATGTAGTAAGGGAAAGAGATAACGACAAAGATGGAAAGCTCAATTTCAGTGAGTTTTTCCCTAAAGTTTTAGACTTAGTAAGAAGAGTGGATGAAGACTACAATTCATCAAATTGGGAGGACGAAGAACCGGAGGCACTGGCAAAAAGGATGTTCTTGGAGCTCGACAAAGACTCTGATGGGTACCTTTCAACAACTGAAATGTTGCCTATAATTGGAAAAATCCATCCATCGGAGGCTTATTATGCAAAACAACAAGCAGAGTACATCATCTCTCAGGCAGATAGTGATGATGATGGACTCCTAACCTTGAACGATATGATCGAGAACCCATCTGTGTTCTATAGCTCTGTTTTTACTGAAGACGAGATGGATTATTATCCATACCACGATGAATTTCGTTAGATGGATACAGTTATTTAAAGAAAAAAAAGATTATGGGAGAAAAGAAAACGACGTTGAGAAAGCTTGAGAATTATCAAGAGATTGATCTTCACAAAATATTAGTTAGAGGTGATTTGCTTTCTTGTTTGTAGCTTAATTAGTTTTATTCTGGCCCTAGCATAGTGTCGGTTTTAATTTCTTGAGTCAGG

Coding sequence (CDS)

ATGGGGAAATTCTCAGTTCTCATTTACATTTCAATCTTTTCCCTTCTTCTAATCCTAATATCTCAAACTCCAACAACCCGCCCCCGTCACCGCCGCCTCCGCCTCCGCTCCAACTTCACCTTCAATCCCCCCCTCCTCCGCCACCAACACGACCACTACATCTCCTTCGACCCTCTCATTTCTGACATTGAGCTCCGCCGTGAGGACGACGAATGGCATAAGCAGTCCCTCAAAAATCTCGCCGCCCATGATTCTCATCCTGAGTGGGAAGAGTTCATCAACGATGAGGACAGGTTTAATGTCACCGAAAGATTGTTTTGGATTTTTCCCAAAATTGATGTGGATCCTTCTGATGGGTTTGTGTCGGCGGAGGAACTGACTCGCTGGAACTTGCAGCAAGCCATGAATGAAGCTTTGTATCGGACTGAAAGGGAATTCCAATCTCATGATGATAATCGTGATGGGTTTGTTTCCTTTGCTGAGTATGAACCCCCCAGTTGGGTTCTTTCTGCCGGTAATGGTTCTTTTGGCTATGATATTGGCTGGTGGAATGAGGAGCACTTCAATGCATCAGATGCAGATGGGGATGGAGTTTTGGATCTAAATGAATTTAATGACTTCTTGCATCCTGCTGACAGCAAGAGCACGAAGCTGCTTCTATGGTTATGTGCAGATGTAGTAAGGGAAAGAGATAACGACAAAGATGGAAAGCTCAATTTCAGTGAGTTTTTCCCTAAAGTTTTAGACTTAGTAAGAAGAGTGGATGAAGACTACAATTCATCAAATTGGGAGGACGAAGAACCGGAGGCACTGGCAAAAAGGATGTTCTTGGAGCTCGACAAAGACTCTGATGGGTACCTTTCAACAACTGAAATGTTGCCTATAATTGGAAAAATCCATCCATCGGAGGCTTATTATGCAAAACAACAAGCAGAGTACATCATCTCTCAGGCAGATAGTGATGATGATGGACTCCTAACCTTGAACGATATGATCGAGAACCCATCTGTGTTCTATAGCTCTGTTTTTACTGAAGACGAGATGGATTATTATCCATACCACGATGAATTTCGTTAG

Protein sequence

MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLISDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPIIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR*

Homology

[Close]

BLAST of CSPI05G20330 vs. ExPASy Swiss-Prot
Match: Q7SXV9 (Calumenin-B OS=Danio rerio OX=7955 GN=calub PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 4.0e-20
Identity = 81/264 (30.68%), Postives = 134/264 (50.76%), Query Frame = 0


Query: 103 ERLFWIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALYR-TEREFQSHDDNRDGFVSFAE 162
           ERL  I  KID D  DGFV+A+E+ RW ++ A    +Y   +R++Q+HD N D FVS+ E
Sbjct: 71  ERLGKIVEKIDED-HDGFVTADEMKRW-IKHAQRRWIYEDVDRQWQAHDLNSDSFVSWEE 130

Query: 163 YEPPS--WVLSAGNGSFGYDIGWW---NEEHFNASDADGDGVLDLNEFNDFLHPADSKST 222
           Y+  +  ++L   +   G++       +E  F  +D DGD   +  EF  FLHP +    
Sbjct: 131 YKDATYGYILDEADPEDGFNYRQMMTRDERRFKMADQDGDLRANKEEFTAFLHPEEFDYM 190

Query: 223 KLLLWLCADVVRERDNDKDGKLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPE--ALAKR 282
           K ++ L  + + + D + DG ++ +E+             D  S N +  EPE     + 
Sbjct: 191 KDIVVL--ETMEDIDKNGDGLIDLNEYI-----------GDMYSQNGDSSEPEWVKTERE 250

Query: 283 MFLEL-DKDSDGYLSTTEMLPIIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIE 342
            F E  DK+ DG +   E       I P++  +A+ +A++++ ++D+D DG LT  ++++
Sbjct: 251 QFTEFRDKNKDGRMDKDETR---DWILPADYDHAEAEAKHLLYESDADKDGRLTKQEIVD 310

Query: 343 NPSVFYSSVFTEDEMDYYPYHDEF 358
              +F  S  T D  D    HDEF
Sbjct: 311 KYDLFVGSQAT-DFGDALVRHDEF 315

[Close]

BLAST of CSPI05G20330 vs. ExPASy Swiss-Prot
Match: J3S9D9 (Reticulocalbin-2 OS=Crotalus adamanteus OX=8729 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 3.2e-17
Identity = 82/303 (27.06%), Postives = 141/303 (46.53%), Query Frame = 0


Query: 71  EWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGFVSAEELTRWN 130
           ++ K++L  L   D   E+ +   +E +    +RL  I  +IDVD  DGF++  EL+ W 
Sbjct: 27  DYDKEAL--LGGQDEVEEFSKLSPEEQQ----KRLKVIISRIDVD-LDGFLTEAELSSWI 86

Query: 131 LQQAMNEALYRTEREFQSHDDNRDGFVSFAEY-----------EPPSWVLSAGNGSFGYD 190
                +  +   +++FQ +D + DG VS+ EY           E  + +  A   SF   
Sbjct: 87  QHSFKSYIIEDAKQQFQHYDKDGDGRVSWEEYNIQMYDRVIDFEEDTTLDDAEEESF-RQ 146

Query: 191 IGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLN 250
           +   +++ F  ++ DGD  LD  EF  F HP ++   K   ++  + + E D D DG ++
Sbjct: 147 LHLKDKKRFQKANKDGDSHLDFEEFAAFEHPEEADYMK--EFVIQESLEEHDKDGDGFVS 206

Query: 251 FSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALA---KRMFLELDKDSDGYLSTTEMLPII 310
             EF             DY       E+PE +     R   + DKD DG LS  E+L   
Sbjct: 207 LQEFL-----------GDYRRDPAAKEDPEWIVVEEDRFKNDYDKDKDGKLSPKELLT-- 266

Query: 311 GKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYP---Y 357
             + P+    A+++A +++ + D D D  L+ N+++EN  +F +S  T+     +    Y
Sbjct: 267 -WVMPNNEGLAQEEAVHLLDEMDLDGDRRLSANEILENQDLFLNSEATDYGRQLHDKSFY 305

[Close]

BLAST of CSPI05G20330 vs. ExPASy Swiss-Prot
Match: Q5RDD8 (Calumenin OS=Pongo abelii OX=9601 GN=CALU PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 4.2e-17
Identity = 78/264 (29.55%), Postives = 128/264 (48.48%), Query Frame = 0


Query: 103 ERLFWIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALYR-TEREFQSHDDNRDGFVSFAE 162
           ERL  I  KID D  DGFV+ +EL  W ++ A    +Y   ER+++ HD N DG VS+ E
Sbjct: 71  ERLGKIVSKID-DDKDGFVTVDELKDW-IKFAQKRWIYEDVERQWKGHDLNEDGLVSWEE 130

Query: 163 YEPPS--WVLSAGNGSFGYDIGWW---NEEHFNASDADGDGVLDLNEFNDFLHPADSKST 222
           Y+  +  +VL   +   G++       +E  F  +D DGD +    EF  FLHP +    
Sbjct: 131 YKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYM 190

Query: 223 KLLLWLCADVVRERDNDKDGKLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPE--ALAKR 282
           K +  +  + + + D + DG ++  E+             D  S +   +EPE     + 
Sbjct: 191 KDI--VVQETMEDIDKNADGFIDLEEYI-----------GDMYSHDGNTDEPEWVKTERE 250

Query: 283 MFLEL-DKDSDGYLSTTEMLPIIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIE 342
            F+E  DK+ DG +   E       I PS+  +A+ +A +++ ++D + DG LT  ++++
Sbjct: 251 QFVEFRDKNRDGKMDKEETK---DWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVD 310

Query: 343 NPSVFYSSVFTEDEMDYYPYHDEF 358
              +F  S  T D  +    HDEF
Sbjct: 311 KYDLFVGSQAT-DFGEALVRHDEF 315

[Close]

BLAST of CSPI05G20330 vs. ExPASy Swiss-Prot
Match: Q6XLQ7 (Calumenin OS=Oryctolagus cuniculus OX=9986 GN=CALU PE=1 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 5.4e-17
Identity = 78/264 (29.55%), Postives = 128/264 (48.48%), Query Frame = 0


Query: 103 ERLFWIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALYR-TEREFQSHDDNRDGFVSFAE 162
           ERL  I  KID D  DGFV+ +EL  W ++ A    +Y   ER+++ HD N DG VS+ E
Sbjct: 71  ERLGKIVSKIDAD-KDGFVTVDELKDW-IKFAQKRWIYEDVERQWKGHDLNEDGLVSWEE 130

Query: 163 YEPPS--WVLSAGNGSFGYDIGWW---NEEHFNASDADGDGVLDLNEFNDFLHPADSKST 222
           Y+  +  +VL   +   G++       +E  F  +D DGD +    EF  FLHP +    
Sbjct: 131 YKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYM 190

Query: 223 KLLLWLCADVVRERDNDKDGKLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPE--ALAKR 282
           K +  +  + + + D + DG ++  E+             D  S +   +EPE     + 
Sbjct: 191 KDI--VVQETMEDIDKNGDGFIDLEEYI-----------GDMYSHDGNADEPEWVKTERE 250

Query: 283 MFLEL-DKDSDGYLSTTEMLPIIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIE 342
            F+E  DK+ DG +   E       I PS+  +A+ +A +++ ++D + DG LT  ++++
Sbjct: 251 QFVEFRDKNRDGKMDKEETK---DWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVD 310

Query: 343 NPSVFYSSVFTEDEMDYYPYHDEF 358
              +F  S  T D  +    HDEF
Sbjct: 311 KYDLFVGSQAT-DFGEALVRHDEF 315

[Close]

BLAST of CSPI05G20330 vs. ExPASy Swiss-Prot
Match: Q28BT4 (Calumenin OS=Xenopus tropicalis OX=8364 GN=calu PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 5.4e-17
Identity = 78/262 (29.77%), Postives = 130/262 (49.62%), Query Frame = 0


Query: 103 ERLFWIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALY-RTEREFQSHDDNRDGFVSFAE 162
           ERL  I  KID+D +DG+V+  ELT W +++A  + +Y   ER++Q  D N+DG VS+ E
Sbjct: 71  ERLGMIVSKIDLD-NDGYVTEGELTAW-IKKAQKKYVYDNVERQWQEFDLNQDGLVSWDE 130

Query: 163 YEPPSWVLSAG----NGSFGY-DIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKST 222
           Y   ++         + SF Y  +   +E  F  +D DGD +    EF  FLHP +    
Sbjct: 131 YRNVTYGTYLDDPDPDNSFNYKQMMVRDERRFKMADQDGDLIATKEEFTAFLHPEEFDYM 190

Query: 223 KLLLWLCADVVRERDNDKDGKLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMF 282
           K ++ L  + + + D + DG ++  E+   + +     D D N   W   E E      F
Sbjct: 191 KDIVVL--ETMEDIDKNGDGLIDLEEYIGDMYN----HDGDANEPEWVKTERE-----QF 250

Query: 283 LEL-DKDSDGYLSTTEMLPIIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENP 342
           +E  DK+ DG +   E       I PS+  +A+ ++ +++ ++D + D  LT  ++++  
Sbjct: 251 VEFRDKNHDGKMDKEETK---DWILPSDYDHAEAESRHLVYESDQNKDSKLTREEIVDKY 310

Query: 343 SVFYSSVFTEDEMDYYPYHDEF 358
            +F  S  T D  +    HDEF
Sbjct: 311 DLFVGSQAT-DFGEALVRHDEF 315

[Close]

BLAST of CSPI05G20330 vs. ExPASy TrEMBL
Match: A0A0A0KT37 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G579050 PE=4 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.4e-209
Identity = 357/358 (99.72%), Postives = 358/358 (100.00%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI 60
           MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI
Sbjct: 1   MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI 60

Query: 61  SDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGF 120
           SDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGF
Sbjct: 61  SDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGF 120

Query: 121 VSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDI 180
           VSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDI
Sbjct: 121 VSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDI 180

Query: 181 GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNF 240
           GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNF
Sbjct: 181 GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNF 240

Query: 241 SEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPIIGKIH 300
           SEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAK+MFLELDKDSDGYLSTTEMLPIIGKIH
Sbjct: 241 SEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKKMFLELDKDSDGYLSTTEMLPIIGKIH 300

Query: 301 PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR 359
           PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR
Sbjct: 301 PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR 358

[Close]

BLAST of CSPI05G20330 vs. ExPASy TrEMBL
Match: A0A5A7SY06 (Calumenin-B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00730 PE=4 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 2.5e-198
Identity = 337/358 (94.13%), Postives = 351/358 (98.04%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI 60
           MGKFSVLIYISIFSLLL+LISQTPTT PRHRRLRLRSNFTFNPPL R QHDHYISFDPLI
Sbjct: 1   MGKFSVLIYISIFSLLLLLISQTPTTVPRHRRLRLRSNFTFNPPLHRQQHDHYISFDPLI 60

Query: 61  SDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGF 120
           SDIELRREDDEW KQSL+NLAAHDSHPEWEEFINDEDRFNVTERLF+IFPKIDVDPSDGF
Sbjct: 61  SDIELRREDDEWQKQSLENLAAHDSHPEWEEFINDEDRFNVTERLFFIFPKIDVDPSDGF 120

Query: 121 VSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDI 180
           VSAEELTRWNLQQAMNEAL+RTEREFQSHD+NRDGFVSFAEYEPPSWVL+AGN SFGYDI
Sbjct: 121 VSAEELTRWNLQQAMNEALHRTEREFQSHDENRDGFVSFAEYEPPSWVLAAGNSSFGYDI 180

Query: 181 GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNF 240
           GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKS KLLLWLCADVVRERDNDKDGKLNF
Sbjct: 181 GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSPKLLLWLCADVVRERDNDKDGKLNF 240

Query: 241 SEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPIIGKIH 300
           SEFFPK+LDL+RRVDEDYNSSNWEDEEPEALA++MFLELDKDSDG+LST+E+LPIIGKIH
Sbjct: 241 SEFFPKILDLIRRVDEDYNSSNWEDEEPEALARKMFLELDKDSDGFLSTSELLPIIGKIH 300

Query: 301 PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR 359
           PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENP VFYSSVF+EDEMDYYPYHDEFR
Sbjct: 301 PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPFVFYSSVFSEDEMDYYPYHDEFR 358

[Close]

BLAST of CSPI05G20330 vs. ExPASy TrEMBL
Match: A0A1S3BDS4 (calumenin-B OS=Cucumis melo OX=3656 GN=LOC103488782 PE=4 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 2.5e-198
Identity = 337/358 (94.13%), Postives = 351/358 (98.04%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI 60
           MGKFSVLIYISIFSLLL+LISQTPTT PRHRRLRLRSNFTFNPPL R QHDHYISFDPLI
Sbjct: 1   MGKFSVLIYISIFSLLLLLISQTPTTVPRHRRLRLRSNFTFNPPLHRQQHDHYISFDPLI 60

Query: 61  SDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGF 120
           SDIELRREDDEW KQSL+NLAAHDSHPEWEEFINDEDRFNVTERLF+IFPKIDVDPSDGF
Sbjct: 61  SDIELRREDDEWQKQSLENLAAHDSHPEWEEFINDEDRFNVTERLFFIFPKIDVDPSDGF 120

Query: 121 VSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDI 180
           VSAEELTRWNLQQAMNEAL+RTEREFQSHD+NRDGFVSFAEYEPPSWVL+AGN SFGYDI
Sbjct: 121 VSAEELTRWNLQQAMNEALHRTEREFQSHDENRDGFVSFAEYEPPSWVLAAGNSSFGYDI 180

Query: 181 GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNF 240
           GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKS KLLLWLCADVVRERDNDKDGKLNF
Sbjct: 181 GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSPKLLLWLCADVVRERDNDKDGKLNF 240

Query: 241 SEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPIIGKIH 300
           SEFFPK+LDL+RRVDEDYNSSNWEDEEPEALA++MFLELDKDSDG+LST+E+LPIIGKIH
Sbjct: 241 SEFFPKILDLIRRVDEDYNSSNWEDEEPEALARKMFLELDKDSDGFLSTSELLPIIGKIH 300

Query: 301 PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR 359
           PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENP VFYSSVF+EDEMDYYPYHDEFR
Sbjct: 301 PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPFVFYSSVFSEDEMDYYPYHDEFR 358

[Close]

BLAST of CSPI05G20330 vs. ExPASy TrEMBL
Match: A0A6J1KJ28 (reticulocalbin-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.4e-161
Identity = 280/362 (77.35%), Postives = 314/362 (86.74%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPTTR--PRHRRLRLRSNFTFNPPLLRHQHDHYISFDP 60
           MGK S+ IYISIFSLLLIL   TP+ R  PRHRRLRLRSNFTFN PL R Q DHY  FDP
Sbjct: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60

Query: 61  LISDIELRREDDEWHKQSL--KNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDP 120
           LI++I+LRRED+EW   SL      A DSHPEWEEFINDEDRFNVTERL  +FP IDV+P
Sbjct: 61  LIANIQLRREDEEWQNHSLGPSQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNP 120

Query: 121 SDGFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSF 180
           +DGF+S EELTRWN QQAMN AL+RTEREF++HD++RDGFVSFAEYEPPSWV++AGN S 
Sbjct: 121 TDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGNSSS 180

Query: 181 GYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDG 240
           GYDIGWWNEEHFNASDADG+G LD++EFNDFLHPADS++ KLLLWLC DVVRERDN KDG
Sbjct: 181 GYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDG 240

Query: 241 KLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPII 300
           KL+FSEF P + DLVRRVDEDY S +W+D++ EA AK+MFLELDKD DGYLS TE+LP+I
Sbjct: 241 KLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPVI 300

Query: 301 GKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDE 359
           GKIHPSEAYYAKQQAEYIISQADSD+D LLTLNDMI+NP VFYSSVF+E EMDYYPYHDE
Sbjct: 301 GKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYYPYHDE 360

[Close]

BLAST of CSPI05G20330 vs. ExPASy TrEMBL
Match: A0A6J1KCI7 (reticulocalbin-2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=4 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 3.5e-160
Identity = 280/363 (77.13%), Postives = 314/363 (86.50%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPTTR--PRHRRLRLRSNFTFNPPLLRHQHDHYISFDP 60
           MGK S+ IYISIFSLLLIL   TP+ R  PRHRRLRLRSNFTFN PL R Q DHY  FDP
Sbjct: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60

Query: 61  LISDIELRREDDEWHKQSL--KNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDP 120
           LI++I+LRRED+EW   SL      A DSHPEWEEFINDEDRFNVTERL  +FP IDV+P
Sbjct: 61  LIANIQLRREDEEWQNHSLGPSQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNP 120

Query: 121 SDGFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAG-NGS 180
           +DGF+S EELTRWN QQAMN AL+RTEREF++HD++RDGFVSFAEYEPPSWV++AG N S
Sbjct: 121 TDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGSNSS 180

Query: 181 FGYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKD 240
            GYDIGWWNEEHFNASDADG+G LD++EFNDFLHPADS++ KLLLWLC DVVRERDN KD
Sbjct: 181 SGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKD 240

Query: 241 GKLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPI 300
           GKL+FSEF P + DLVRRVDEDY S +W+D++ EA AK+MFLELDKD DGYLS TE+LP+
Sbjct: 241 GKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPV 300

Query: 301 IGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHD 359
           IGKIHPSEAYYAKQQAEYIISQADSD+D LLTLNDMI+NP VFYSSVF+E EMDYYPYHD
Sbjct: 301 IGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYYPYHD 360

[Close]

BLAST of CSPI05G20330 vs. NCBI nr
Match: XP_011655504.1 (calumenin-B [Cucumis sativus] >XP_031741815.1 calumenin-B [Cucumis sativus] >KGN51567.1 hypothetical protein Csa_009120 [Cucumis sativus])

HSP 1 Score: 738.4 bits (1905), Expect = 2.9e-209
Identity = 357/358 (99.72%), Postives = 358/358 (100.00%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI 60
           MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI
Sbjct: 1   MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI 60

Query: 61  SDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGF 120
           SDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGF
Sbjct: 61  SDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGF 120

Query: 121 VSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDI 180
           VSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDI
Sbjct: 121 VSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDI 180

Query: 181 GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNF 240
           GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNF
Sbjct: 181 GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNF 240

Query: 241 SEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPIIGKIH 300
           SEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAK+MFLELDKDSDGYLSTTEMLPIIGKIH
Sbjct: 241 SEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKKMFLELDKDSDGYLSTTEMLPIIGKIH 300

Query: 301 PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR 359
           PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR
Sbjct: 301 PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR 358

[Close]

BLAST of CSPI05G20330 vs. NCBI nr
Match: XP_008445899.1 (PREDICTED: calumenin-B [Cucumis melo] >KAA0034085.1 calumenin-B [Cucumis melo var. makuwa])

HSP 1 Score: 701.0 bits (1808), Expect = 5.1e-198
Identity = 337/358 (94.13%), Postives = 351/358 (98.04%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPTTRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDPLI 60
           MGKFSVLIYISIFSLLL+LISQTPTT PRHRRLRLRSNFTFNPPL R QHDHYISFDPLI
Sbjct: 1   MGKFSVLIYISIFSLLLLLISQTPTTVPRHRRLRLRSNFTFNPPLHRQQHDHYISFDPLI 60

Query: 61  SDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSDGF 120
           SDIELRREDDEW KQSL+NLAAHDSHPEWEEFINDEDRFNVTERLF+IFPKIDVDPSDGF
Sbjct: 61  SDIELRREDDEWQKQSLENLAAHDSHPEWEEFINDEDRFNVTERLFFIFPKIDVDPSDGF 120

Query: 121 VSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDI 180
           VSAEELTRWNLQQAMNEAL+RTEREFQSHD+NRDGFVSFAEYEPPSWVL+AGN SFGYDI
Sbjct: 121 VSAEELTRWNLQQAMNEALHRTEREFQSHDENRDGFVSFAEYEPPSWVLAAGNSSFGYDI 180

Query: 181 GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNF 240
           GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKS KLLLWLCADVVRERDNDKDGKLNF
Sbjct: 181 GWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSPKLLLWLCADVVRERDNDKDGKLNF 240

Query: 241 SEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPIIGKIH 300
           SEFFPK+LDL+RRVDEDYNSSNWEDEEPEALA++MFLELDKDSDG+LST+E+LPIIGKIH
Sbjct: 241 SEFFPKILDLIRRVDEDYNSSNWEDEEPEALARKMFLELDKDSDGFLSTSELLPIIGKIH 300

Query: 301 PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR 359
           PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENP VFYSSVF+EDEMDYYPYHDEFR
Sbjct: 301 PSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPFVFYSSVFSEDEMDYYPYHDEFR 358

[Close]

BLAST of CSPI05G20330 vs. NCBI nr
Match: XP_038892509.1 (calumenin-B-like [Benincasa hispida] >XP_038892510.1 calumenin-B-like [Benincasa hispida])

HSP 1 Score: 646.4 bits (1666), Expect = 1.5e-181
Identity = 312/360 (86.67%), Postives = 335/360 (93.06%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPT--TRPRHRRLRLRSNFTFNPPLLRHQHDHYISFDP 60
           MGK ++LIYISIFSLLLILIS TPT   R RHRRLRLRSNFTFNP L R QHDHYI FDP
Sbjct: 1   MGKLAILIYISIFSLLLILISHTPTRRLRLRHRRLRLRSNFTFNPLLHRQQHDHYIPFDP 60

Query: 61  LISDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDPSD 120
           LI+DI+LRREDDEW K SL++ AA DSHPEWEEFINDEDRFNVTERLFW+FPKIDVDPSD
Sbjct: 61  LIADIQLRREDDEWQKHSLQHSAAADSHPEWEEFINDEDRFNVTERLFWLFPKIDVDPSD 120

Query: 121 GFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGY 180
           GFVSAEELTRWNLQQAMNEAL+RTE+EFQSHDDNRDGFVSFAEYEPP+WVL+AGN SFGY
Sbjct: 121 GFVSAEELTRWNLQQAMNEALHRTEKEFQSHDDNRDGFVSFAEYEPPTWVLAAGNDSFGY 180

Query: 181 DIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKL 240
           DIGWWNEEHFNASDADGDGVLD+NEFNDFLHPADSKS KLLLWLCADVVRERDNDKDGKL
Sbjct: 181 DIGWWNEEHFNASDADGDGVLDINEFNDFLHPADSKSPKLLLWLCADVVRERDNDKDGKL 240

Query: 241 NFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPIIGK 300
           NFSEFFP++ DLVRRVDEDY+S NW+D+E EA AK+MF  LDK+SDGYLSTTE+LPIIGK
Sbjct: 241 NFSEFFPRIFDLVRRVDEDYSSLNWDDDELEARAKKMFSNLDKNSDGYLSTTELLPIIGK 300

Query: 301 IHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDEFR 359
           IHP EAYYAKQQAEYIIS ADSD+DGLLTLNDMI+NP VFYSSVF+EDEMDYYPYHDEFR
Sbjct: 301 IHPPEAYYAKQQAEYIISLADSDNDGLLTLNDMIDNPFVFYSSVFSEDEMDYYPYHDEFR 360

[Close]

BLAST of CSPI05G20330 vs. NCBI nr
Match: XP_022999248.1 (reticulocalbin-2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 578.9 bits (1491), Expect = 2.9e-161
Identity = 280/362 (77.35%), Postives = 314/362 (86.74%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPTTR--PRHRRLRLRSNFTFNPPLLRHQHDHYISFDP 60
           MGK S+ IYISIFSLLLIL   TP+ R  PRHRRLRLRSNFTFN PL R Q DHY  FDP
Sbjct: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60

Query: 61  LISDIELRREDDEWHKQSL--KNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDP 120
           LI++I+LRRED+EW   SL      A DSHPEWEEFINDEDRFNVTERL  +FP IDV+P
Sbjct: 61  LIANIQLRREDEEWQNHSLGPSQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNP 120

Query: 121 SDGFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSF 180
           +DGF+S EELTRWN QQAMN AL+RTEREF++HD++RDGFVSFAEYEPPSWV++AGN S 
Sbjct: 121 TDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGNSSS 180

Query: 181 GYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDG 240
           GYDIGWWNEEHFNASDADG+G LD++EFNDFLHPADS++ KLLLWLC DVVRERDN KDG
Sbjct: 181 GYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKDG 240

Query: 241 KLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPII 300
           KL+FSEF P + DLVRRVDEDY S +W+D++ EA AK+MFLELDKD DGYLS TE+LP+I
Sbjct: 241 KLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPVI 300

Query: 301 GKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHDE 359
           GKIHPSEAYYAKQQAEYIISQADSD+D LLTLNDMI+NP VFYSSVF+E EMDYYPYHDE
Sbjct: 301 GKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYYPYHDE 360

[Close]

BLAST of CSPI05G20330 vs. NCBI nr
Match: XP_022999241.1 (reticulocalbin-2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 574.3 bits (1479), Expect = 7.2e-160
Identity = 280/363 (77.13%), Postives = 314/363 (86.50%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPTTR--PRHRRLRLRSNFTFNPPLLRHQHDHYISFDP 60
           MGK S+ IYISIFSLLLIL   TP+ R  PRHRRLRLRSNFTFN PL R Q DHY  FDP
Sbjct: 1   MGKLSIFIYISIFSLLLILFFHTPSRRITPRHRRLRLRSNFTFNQPLHRQQQDHYAPFDP 60

Query: 61  LISDIELRREDDEWHKQSL--KNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVDP 120
           LI++I+LRRED+EW   SL      A DSHPEWEEFINDEDRFNVTERL  +FP IDV+P
Sbjct: 61  LIANIQLRREDEEWQNHSLGPSQSDAADSHPEWEEFINDEDRFNVTERLSSLFPNIDVNP 120

Query: 121 SDGFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAG-NGS 180
           +DGF+S EELTRWN QQAMN AL+RTEREF++HD++RDGFVSFAEYEPPSWV++AG N S
Sbjct: 121 TDGFISVEELTRWNWQQAMNAALHRTEREFETHDEDRDGFVSFAEYEPPSWVMAAGSNSS 180

Query: 181 FGYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKD 240
            GYDIGWWNEEHFNASDADG+G LD++EFNDFLHPADS++ KLLLWLC DVVRERDN KD
Sbjct: 181 SGYDIGWWNEEHFNASDADGNGALDMSEFNDFLHPADSRNPKLLLWLCRDVVRERDNSKD 240

Query: 241 GKLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPI 300
           GKL+FSEF P + DLVRRVDEDY S +W+D++ EA AK+MFLELDKD DGYLS TE+LP+
Sbjct: 241 GKLSFSEFSPAIFDLVRRVDEDYGSLSWDDDQMEARAKKMFLELDKDEDGYLSATELLPV 300

Query: 301 IGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTEDEMDYYPYHD 359
           IGKIHPSEAYYAKQQAEYIISQADSD+D LLTLNDMI+NP VFYSSVF+E EMDYYPYHD
Sbjct: 301 IGKIHPSEAYYAKQQAEYIISQADSDNDELLTLNDMIDNPFVFYSSVFSEYEMDYYPYHD 360

[Close]

BLAST of CSPI05G20330 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 415.2 bits (1066), Expect = 5.2e-116
Identity = 210/396 (53.03%), Postives = 278/396 (70.20%), Query Frame = 0


Query: 1   MGKFSVLIYISIFSLLLILISQTPTTR----------PRHRRLRLRSNFTFNPPLLRHQH 60
           M K SV++YI++  L+L L+S +P  +           +H RL+LRS+F F P     +H
Sbjct: 1   MSKASVILYITVGILVLFLVSYSPKKKGDHDHHHGGHNQHHRLKLRSSFNFKPT----RH 60

Query: 61  DHYISFDPLISDIELRREDDEWHKQSL--------------------KNLAAHDSHPEWE 120
           D  + FDPL++D+E RRED EW +Q +                    ++   H+S PEWE
Sbjct: 61  DP-VPFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEWE 120

Query: 121 EF------INDEDRFNVTERLFWIFPKIDVDPSDGFVSAEELTRWNLQQAMNEALYRTER 180
           EF      +NDE++FNVT+RL  +FPKIDV P+DGF++  ELT W +Q +  E ++RT+R
Sbjct: 121 EFMDAEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQR 180

Query: 181 EFQSHDDNRDGFVSFAEYEPPSWVLSAGNGSFGYDIGWWNEEHFNASDADGDGVLDLNEF 240
           +   HD N+DGF+SF+EYEPPSWV  + N SFGYD+GWW EEHFNASDA+GDG+L+L EF
Sbjct: 181 DLDVHDRNKDGFISFSEYEPPSWVRKSDNNSFGYDMGWWKEEHFNASDANGDGLLNLTEF 240

Query: 241 NDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNFSEFFPKVLDLVRRVDED-YNSSNW 300
           NDFLHPAD+K+ KLLLWLC + VRERD+DKDGK++F EFF  + D VR  +ED +NS++ 
Sbjct: 241 NDFLHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTHP 300

Query: 301 EDEEPEALAKRMFLELDKDSDGYLSTTEMLPIIGKIHPSEAYYAKQQAEYIISQADSDDD 359
             + PE  AK++F +LDK+ DGYLS  E+LPII KIHP+E YYAKQQA+YIISQADSD D
Sbjct: 301 YHDLPEGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDKD 360

[Close]

BLAST of CSPI05G20330 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 188.3 bits (477), Expect = 1.0e-47
Identity = 104/304 (34.21%), Postives = 167/304 (54.93%), Query Frame = 0


Query: 56  FDPLISDIELRREDDEWHKQSLKNLAAHDSHPEWEEFINDEDRFNVTERLFWIFPKIDVD 115
           FDPL++ IE    + E   ++++  A  +    +EE+   E R N T R+ ++FP +D  
Sbjct: 48  FDPLVTRIERLSHEKEAGTKTVE-AAKEEKDDMFEEYFAQERRLNTTMRIKFLFPLLDAS 107

Query: 116 PSDGFVSAEELTRWNLQQAMNEALYRTEREFQSHDDNRDGFVSFAEYEPPSWVLSAGNGS 175
           P DGFVS +EL  W +QQ  +  +YRT +E +  D ++DG ++F EY P           
Sbjct: 108 PRDGFVSLKELQTWMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYLPQFSKQDIEKNE 167

Query: 176 FGY-DIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDK 235
            G+ + GWW E+ F  SD D +G LD+ EFN+FLHP DS++     W+  + +   D + 
Sbjct: 168 KGHGEAGWWMEQ-FKNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWVLKERMTGMDTNG 227

Query: 236 DGKLNFSEFFPKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLP 295
           DGKL + EF     ++ +    ++     E++E     + +F E+D+D D +L   E+ P
Sbjct: 228 DGKLEYKEFVKNAYEMYK----EFAKFEKEEDENVPTPQLLFAEMDRDKDRFLVADELRP 287

Query: 296 IIGKIHPSEAYYAKQQAEYIISQADSDDDGLLTLNDMIENPSVFYSSVFTE--DEMDYYP 355
           I+  + P E  YAK  + ++  +AD D DG L+L +M+ +  VFY +V  E  D+ DY+ 
Sbjct: 288 ILQYLQPGEMSYAKFYSTFLCHEADEDKDGKLSLEEMLHHEDVFYKAVHHEDLDDEDYFD 344

Query: 356 YHDE 357
            HDE
Sbjct: 348 -HDE 344

[Close]

BLAST of CSPI05G20330 vs. TAIR 10
Match: AT4G14640.1 (calmodulin 8 )

HSP 1 Score: 57.0 bits (136), Expect = 3.6e-08
Identity = 40/149 (26.85%), Postives = 73/149 (48.99%), Query Frame = 0


Query: 185 EEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDNDKDGKLNFSEFF 244
           +E F   D DGDG + + E    +   D   T+  L    D++ E D+D +G + F+EF 
Sbjct: 15  KEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQEL---HDIITEIDSDSNGTIEFAEF- 74

Query: 245 PKVLDLVRRVDEDYNSSNWEDEEPEALAKRMFLELDKDSDGYLSTTEMLPIIGKIHPSEA 304
              L+L+        +   ++ + E   K  F   DKD +GY+S +E+  ++  I+  E 
Sbjct: 75  ---LNLM--------AKKLQESDAEEELKEAFKVFDKDQNGYISASELSHVM--INLGEK 134

Query: 305 YYAKQQAEYIISQADSDDDGLLTLNDMIE 334
               ++ E +I +AD D DG +  ++ ++
Sbjct: 135 -LTDEEVEQMIKEADLDGDGQVNYDEFVK 145

[Close]

BLAST of CSPI05G20330 vs. TAIR 10
Match: AT4G13440.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 43.1 bits (100), Expect = 5.4e-04
Identity = 26/70 (37.14%), Postives = 37/70 (52.86%), Query Frame = 0


Query: 173 NGSFGYDIGWWNEEHFNASDADGDGVLDLNEFNDFLHPADSKSTKLLLWLCADVVRERDN 232
           NGS       W  + F A D +GDG + L E+ DFL     + T  L W+  ++ RE D 
Sbjct: 14  NGSMEQQNLSW--QFFRAMDVNGDGRVSLQEYTDFL-----RQTAGLAWIHPEMFRELDR 73

Query: 233 DKDGKLNFSE 243
           + DG+L+F E
Sbjct: 74  NGDGQLDFWE 76

The following BLAST results are available for this feature:

BLAST of CSPI05G20330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7SXV9	4.0e-20	30.68	Calumenin-B OS=Danio rerio OX=7955 GN=calub PE=2 SV=1	[more]
J3S9D9	3.2e-17	27.06	Reticulocalbin-2 OS=Crotalus adamanteus OX=8729 PE=1 SV=1	[more]
Q5RDD8	4.2e-17	29.55	Calumenin OS=Pongo abelii OX=9601 GN=CALU PE=2 SV=1	[more]
Q6XLQ7	5.4e-17	29.55	Calumenin OS=Oryctolagus cuniculus OX=9986 GN=CALU PE=1 SV=2	[more]
Q28BT4	5.4e-17	29.77	Calumenin OS=Xenopus tropicalis OX=8364 GN=calu PE=2 SV=1	[more]

BLAST of CSPI05G20330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KT37	1.4e-209	99.72	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G579050 PE=4 SV=1	[more]
A0A5A7SY06	2.5e-198	94.13	Calumenin-B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00730 PE=...	[more]
A0A1S3BDS4	2.5e-198	94.13	calumenin-B OS=Cucumis melo OX=3656 GN=LOC103488782 PE=4 SV=1	[more]
A0A6J1KJ28	1.4e-161	77.35	reticulocalbin-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=...	[more]
A0A6J1KCI7	3.5e-160	77.13	reticulocalbin-2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493671 PE=...	[more]

BLAST of CSPI05G20330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655504.1	2.9e-209	99.72	calumenin-B [Cucumis sativus] >XP_031741815.1 calumenin-B [Cucumis sativus] >KGN...	[more]
XP_008445899.1	5.1e-198	94.13	PREDICTED: calumenin-B [Cucumis melo] >KAA0034085.1 calumenin-B [Cucumis melo va...	[more]
XP_038892509.1	1.5e-181	86.67	calumenin-B-like [Benincasa hispida] >XP_038892510.1 calumenin-B-like [Benincasa...	[more]
XP_022999248.1	2.9e-161	77.35	reticulocalbin-2-like isoform X2 [Cucurbita maxima]	[more]
XP_022999241.1	7.2e-160	77.13	reticulocalbin-2-like isoform X1 [Cucurbita maxima]	[more]

BLAST of CSPI05G20330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08580.1	5.2e-116	53.03	Calcium-binding EF hand family protein	[more]
AT4G27790.1	1.0e-47	34.21	Calcium-binding EF hand family protein	[more]
AT4G14640.1	3.6e-08	26.85	calmodulin 8	[more]
AT4G13440.1	5.4e-04	37.14	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 183..211 e-value: 5.2 score: 12.0 coord: 271..299 e-value: 0.56 score: 19.2 coord: 222..250 e-value: 130.0 score: 0.7
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 187..207 e-value: 0.0081 score: 15.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 272..332 e-value: 4.4E-8 score: 33.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 267..302 score: 11.305434
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 185..214 score: 10.608049
None	No IPR available	GENE3D	1.10.238.10		coord: 251..336 e-value: 6.5E-11 score: 44.3
None	No IPR available	GENE3D	1.10.238.10		coord: 101..163 e-value: 5.4E-7 score: 31.6
None	No IPR available	GENE3D	1.10.238.10		coord: 179..245 e-value: 1.0E-8 score: 37.3
None	No IPR available	PANTHER	PTHR10827:SF83	BNAC09G47400D PROTEIN	coord: 2..358
None	No IPR available	PANTHER	PTHR10827	RETICULOCALBIN	coord: 2..358
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 192..204
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 231..243
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 280..292
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 150..162
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 319..331
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 101..162
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 186..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G20330.1	CSPI05G20330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding