CSPI05G19710 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G19710
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	heavy metal-associated isoprenylated plant protein 22
Location	Chr5: 20922125 .. 20923177 (+)
RNA-Seq Expression	CSPI05G19710
Synteny	CSPI05G19710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTATGTTTATAGATGTGTATATATGGCCACATAGTTCATTAATTCCACTGTGCTCCCAGAAAAAAAAAATGGGAATTGTTGGTTTTGTTTCAGATTATGTAACAGATAATCTTGGTTCAAGAAAAAAGAAGAGAAAGCCAAACCAGGTAATTGATTCATACACACACATATATAGATATGTTTGTTAATTTGTTAATTATTATGATAATATATCAACTTTATATGTTTGCTTTTTGTGATTTTACATAGACAGTAGAAATCAAGGTGAAGATGGACTGTGATGGGTGTGAGAGGAGAATTAAAAATGCTGTTTCTTCTGTCAAAGGTCTCCTATTTTCTAATATACTAATTAATTTCATCTCTAATCTTTGTCATTTGTTTGAAAATACTCTTATTTCCTTTTGGAGTTTCAACATTACTATAGATTGAAACTTGGTAGTACGAATGTGACAATGACTTGAGGTTGATATAATGGTGTTTAATAATTAGGTCTAATTTTCGTTCTAGGTTATTATCATAGAAATCTAAAGTTTGGTGTACTGCTTAGTTTTCTTGGGAATTTGTCTCCATATGTTTTGAAAAGAGATTTTGAGACTTTTTTCTTTTTTTTGTTAATGAATGAGAATTAAGGAGTGAAGTCAGTGAAGGTGGACAGAAAGCAAAGCAAAGTAACAGTAAATGGATATGCTGAGGCAACCAAAGTTTTAAAAAAGGTTGAAAGCACGGGAAAGAAGGCTGAGCTTTGGCCTTATGTTCCCTACAACTCTGTGGCTTATCCTTATGTACCTCAAGCCTACGACAAGAAGGCGCCCCCTGGCTACGTCAAGAAGGCCCCTCAAGCCCTTCCCGTCGACGAGGCACTCGATCAGAGGTTGACGATGATGTTTAGTGATGAAAATCCTAATGCATGTTCTATTATGTAGCAAAATATATGTATAAATATGGTTGTTTGGCTTTATCAACGACCTTTCAACCATGCATGGAAGAAGATTATAATTAATAGAGTTTCTATACGTATATATATGTATGTATTCGACAACATTTTGAGGGGG

mRNA sequence

GGTATGTTTATAGATGTGTATATATGGCCACATAGTTCATTAATTCCACTGTGCTCCCAGAAAAAAAAAATGGGAATTGTTGGTTTTGTTTCAGATTATGTAACAGATAATCTTGGTTCAAGAAAAAAGAAGAGAAAGCCAAACCAGACAGTAGAAATCAAGGTGAAGATGGACTGTGATGGGTGTGAGAGGAGAATTAAAAATGCTGTTTCTTCTGTCAAAGGAGTGAAGTCAGTGAAGGTGGACAGAAAGCAAAGCAAAGTAACAGTAAATGGATATGCTGAGGCAACCAAAGTTTTAAAAAAGGTTGAAAGCACGGGAAAGAAGGCTGAGCTTTGGCCTTATGTTCCCTACAACTCTGTGGCTTATCCTTATGTACCTCAAGCCTACGACAAGAAGGCGCCCCCTGGCTACGTCAAGAAGGCCCCTCAAGCCCTTCCCGTCGACGAGGCACTCGATCAGAGGTTGACGATGATGTTTAGTGATGAAAATCCTAATGCATGTTCTATTATGTAGCAAAATATATGTATAAATATGGTTGTTTGGCTTTATCAACGACCTTTCAACCATGCATGGAAGAAGATTATAATTAATAGAGTTTCTATACGTATATATATGTATGTATTCGACAACATTTTGAGGGGG

Coding sequence (CDS)

ATGTTTATAGATGTGTATATATGGCCACATAGTTCATTAATTCCACTGTGCTCCCAGAAAAAAAAAATGGGAATTGTTGGTTTTGTTTCAGATTATGTAACAGATAATCTTGGTTCAAGAAAAAAGAAGAGAAAGCCAAACCAGACAGTAGAAATCAAGGTGAAGATGGACTGTGATGGGTGTGAGAGGAGAATTAAAAATGCTGTTTCTTCTGTCAAAGGAGTGAAGTCAGTGAAGGTGGACAGAAAGCAAAGCAAAGTAACAGTAAATGGATATGCTGAGGCAACCAAAGTTTTAAAAAAGGTTGAAAGCACGGGAAAGAAGGCTGAGCTTTGGCCTTATGTTCCCTACAACTCTGTGGCTTATCCTTATGTACCTCAAGCCTACGACAAGAAGGCGCCCCCTGGCTACGTCAAGAAGGCCCCTCAAGCCCTTCCCGTCGACGAGGCACTCGATCAGAGGTTGACGATGATGTTTAGTGATGAAAATCCTAATGCATGTTCTATTATGTAG

Protein sequence

MFIDVYIWPHSSLIPLCSQKKKMGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDRKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAPQALPVDEALDQRLTMMFSDENPNACSIM*

Homology

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BLAST of CSPI05G19710 vs. ExPASy Swiss-Prot
Match: Q93VP2 (Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana OX=3702 GN=HIPP22 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 6.5e-45
Identity = 91/152 (59.87%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLG---SRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVK 82
           MG + ++S+Y +++      ++KKRK  QTV IKVK+DCDGCER+IKNAVSS+KG KSV+
Sbjct: 1   MGALNYLSEYFSNHFYVSIRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVE 60

Query: 83  VDRKQSKVTVNGYAEATKVLKKVESTG-KKAELWPYVPYNSVAYPYVPQAYDKKAPPGYV 142
           V+RK  KVTV+GY +  KVLK V+STG KKAELWPYVPY  VAYPY   AYDK+APPG+V
Sbjct: 61  VNRKMHKVTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFV 120

Query: 143 KKAPQALPVDEALDQRLTMMFSDENPNACSIM 171
           +K+ QA     + D +L  +FSDENPNAC++M
Sbjct: 121 RKSEQAQAQPGSTDDKLMSLFSDENPNACTVM 152

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BLAST of CSPI05G19710 vs. ExPASy Swiss-Prot
Match: Q9C9A3 (Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana OX=3702 GN=HIPP20 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 1.9e-44
Identity = 93/154 (60.39%), Postives = 117/154 (75.97%), Query Frame = 0


Query: 23  MGIVGFVSDYVTD--NLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKV 82
           MG +  +S+Y++D   +  +++KRK  QTV IKVKMDCDGCERR+KNAVSS+KGVKSV+V
Sbjct: 1   MGALDSLSEYISDYFRVTRKRRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEV 60

Query: 83  DRKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKK 142
           +RK  KVTV+GY E  KVLK++E TGKKAE+WPYVPYN VAYPY    YDKKAP GYV+K
Sbjct: 61  NRKIHKVTVSGYVEPKKVLKRIERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVRK 120

Query: 143 APQA----LPVDEALDQRLTMMFSDENPNACSIM 171
           + Q+    LP   A +     +FSDENPNAC++M
Sbjct: 121 SEQSQLQLLP--GAPENHYISLFSDENPNACTVM 152

[Close]

BLAST of CSPI05G19710 vs. ExPASy Swiss-Prot
Match: O65657 (Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX=3702 GN=HIPP23 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.6e-43
Identity = 89/156 (57.05%), Postives = 120/156 (76.92%), Query Frame = 0


Query: 26  VGFVSDYVTDNLGS-------RKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSV 85
           VG   +Y+++ +G+       ++KK+K  QTVE+KV+MDCDGC  +IKN++SS+KGVK+V
Sbjct: 3   VGGTLEYISELIGNGGSHSYGKRKKKKQFQTVELKVRMDCDGCVLKIKNSLSSLKGVKTV 62

Query: 86  KVDRKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYV 145
           ++++KQ KVTV+GYA+A+KVLKK ++TGKKAE+WPYVPYN VA PY+ QAYDKKAPPGYV
Sbjct: 63  EINKKQQKVTVSGYADASKVLKKAKATGKKAEIWPYVPYNLVAQPYIAQAYDKKAPPGYV 122

Query: 146 KKA-PQALPVDEAL---DQRLTMMFSDENPNACSIM 171
           +K  P       A+   D   T +FSD+NPNACSIM
Sbjct: 123 RKVDPNVTTGTMAVYYDDPSYTSLFSDDNPNACSIM 158

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BLAST of CSPI05G19710 vs. ExPASy Swiss-Prot
Match: Q9LF57 (Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana OX=3702 GN=HIPP21 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 9.8e-41
Identity = 79/149 (53.02%), Postives = 109/149 (73.15%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR 82
           MG   ++S + +    + K KRKP QTV+IKVKMDCDGCERR++N V  +KGVKSV+V+R
Sbjct: 1   MGAFDYISSFCSYTYANAKTKRKPLQTVDIKVKMDCDGCERRVRNVVRRMKGVKSVEVNR 60

Query: 83  KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP 142
           KQS++TVNG+ +  KVLK+V+STGKKAE WPY+P + V YP+ P  YDK+AP G+++   
Sbjct: 61  KQSRITVNGHVDPNKVLKRVKSTGKKAEFWPYIPQHMVYYPFAPGMYDKRAPAGHIRNPT 120

Query: 143 QALPVDEALDQRLTMMFSDENPN-ACSIM 171
           Q+ P   A ++    +FSD+N + ACSIM
Sbjct: 121 QSFPTANAPEENYVSLFSDDNVHAACSIM 149

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BLAST of CSPI05G19710 vs. ExPASy Swiss-Prot
Match: O81464 (Heavy metal-associated isoprenylated plant protein 24 OS=Arabidopsis thaliana OX=3702 GN=HIPP24 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 9.1e-39
Identity = 86/151 (56.95%), Postives = 109/151 (72.19%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSRK-KKRKPNQTVEIKV-KMDCDGCERRIKNAVSSVKGVKSVKV 82
           MG+ G   +Y++D L  RK KK+K  QTV ++V ++DC+GCER+IK+ +S VKGVKSV V
Sbjct: 1   MGVEG-TMEYISDLLKKRKRKKKKQMQTVALRVARIDCEGCERKIKHVLSGVKGVKSVDV 60

Query: 83  DRKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKK 142
           D K  KVTV GY +  KVL+  +ST KK ELWPYVPY  VA PY+ QAYDKKAPP  V+K
Sbjct: 61  DVKLQKVTVTGYIDPKKVLEAAKSTKKKVELWPYVPYTMVANPYISQAYDKKAPPNMVRK 120

Query: 143 APQALPVDE-ALDQRLTMMFSDENPNACSIM 171
            P    V+E  +D   T+MFSDENPN+C+IM
Sbjct: 121 VPDTASVNETTVDDSYTIMFSDENPNSCAIM 150

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BLAST of CSPI05G19710 vs. ExPASy TrEMBL
Match: A0A0A0KPL8 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G571510 PE=4 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 6.7e-77
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR 82
           MGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR
Sbjct: 1   MGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR 60

Query: 83  KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP 142
           KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP
Sbjct: 61  KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP 120

Query: 143 QALPVDEALDQRLTMMFSDENPNACSIM 171
           QALPVDEALDQRLTMMFSDENPNACSIM
Sbjct: 121 QALPVDEALDQRLTMMFSDENPNACSIM 148

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BLAST of CSPI05G19710 vs. ExPASy TrEMBL
Match: A0A5D3E3U3 (Heavy metal-associated isoprenylated plant protein 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00200 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.0e-76
Identity = 147/148 (99.32%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR 82
           MGIVGFVSDYVTDN GSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR
Sbjct: 1   MGIVGFVSDYVTDNFGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR 60

Query: 83  KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP 142
           KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP
Sbjct: 61  KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP 120

Query: 143 QALPVDEALDQRLTMMFSDENPNACSIM 171
           QALPVDEALDQRLTMMFSDENPNACSIM
Sbjct: 121 QALPVDEALDQRLTMMFSDENPNACSIM 148

[Close]

BLAST of CSPI05G19710 vs. ExPASy TrEMBL
Match: A0A1S3CSV0 (heavy metal-associated isoprenylated plant protein 22 OS=Cucumis melo OX=3656 GN=LOC103504570 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.0e-76
Identity = 147/148 (99.32%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR 82
           MGIVGFVSDYVTDN GSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR
Sbjct: 1   MGIVGFVSDYVTDNFGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR 60

Query: 83  KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP 142
           KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP
Sbjct: 61  KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP 120

Query: 143 QALPVDEALDQRLTMMFSDENPNACSIM 171
           QALPVDEALDQRLTMMFSDENPNACSIM
Sbjct: 121 QALPVDEALDQRLTMMFSDENPNACSIM 148

[Close]

BLAST of CSPI05G19710 vs. ExPASy TrEMBL
Match: A0A6J1FG16 (heavy metal-associated isoprenylated plant protein 22-like OS=Cucurbita moschata OX=3662 GN=LOC111445372 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 3.5e-65
Identity = 131/149 (87.92%), Postives = 138/149 (92.62%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSR-KKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVD 82
           MGI  FVSD++ DNLGSR KKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSV+V+
Sbjct: 1   MGIADFVSDWLIDNLGSRKKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVEVN 60

Query: 83  RKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKA 142
           RKQSKVT  GYAE +KVLKKV+STGKKAELWPYVPY+ VAYPYV QAYDKKAPPGYVKKA
Sbjct: 61  RKQSKVTAVGYAEPSKVLKKVQSTGKKAELWPYVPYSVVAYPYVAQAYDKKAPPGYVKKA 120

Query: 143 PQALPVDEALDQRLTMMFSDENPNACSIM 171
           PQA PVDEA DQRLTMMFSDENPNACSIM
Sbjct: 121 PQAFPVDEAPDQRLTMMFSDENPNACSIM 149

[Close]

BLAST of CSPI05G19710 vs. ExPASy TrEMBL
Match: A0A6J1JVQ2 (heavy metal-associated isoprenylated plant protein 22-like OS=Cucurbita maxima OX=3661 GN=LOC111489303 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 5.0e-64
Identity = 130/149 (87.25%), Postives = 137/149 (91.95%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSR-KKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVD 82
           MGI  FVSD++TDNLGSR KKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSV+V+
Sbjct: 1   MGIPDFVSDWLTDNLGSRKKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVEVN 60

Query: 83  RKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKA 142
           RKQSKVT  GYAE +KVLKKV+STGKKAELWPYVPY+ VAYPYV QAYDKKAPPGYVK A
Sbjct: 61  RKQSKVTAVGYAEPSKVLKKVQSTGKKAELWPYVPYSVVAYPYVAQAYDKKAPPGYVKNA 120

Query: 143 PQALPVDEALDQRLTMMFSDENPNACSIM 171
            QA PVDEA DQRLTMMFSDENPNACSIM
Sbjct: 121 VQAFPVDEAPDQRLTMMFSDENPNACSIM 149

[Close]

BLAST of CSPI05G19710 vs. NCBI nr
Match: XP_004150477.2 (heavy metal-associated isoprenylated plant protein 22 [Cucumis sativus] >KGN52094.2 hypothetical protein Csa_008913 [Cucumis sativus])

HSP 1 Score: 344.4 bits (882), Expect = 5.8e-91
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0


Query: 1   MFIDVYIWPHSSLIPLCSQKKKMGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDG 60
           MFIDVYIWPHSSLIPLCSQKKKMGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDG
Sbjct: 1   MFIDVYIWPHSSLIPLCSQKKKMGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDG 60

Query: 61  CERRIKNAVSSVKGVKSVKVDRKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSV 120
           CERRIKNAVSSVKGVKSVKVDRKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSV
Sbjct: 61  CERRIKNAVSSVKGVKSVKVDRKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSV 120

Query: 121 AYPYVPQAYDKKAPPGYVKKAPQALPVDEALDQRLTMMFSDENPNACSIM 171
           AYPYVPQAYDKKAPPGYVKKAPQALPVDEALDQRLTMMFSDENPNACSIM
Sbjct: 121 AYPYVPQAYDKKAPPGYVKKAPQALPVDEALDQRLTMMFSDENPNACSIM 170

[Close]

BLAST of CSPI05G19710 vs. NCBI nr
Match: XP_008467147.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 22 [Cucumis melo] >KAA0046312.1 heavy metal-associated isoprenylated plant protein 22 [Cucumis melo var. makuwa] >TYK30478.1 heavy metal-associated isoprenylated plant protein 22 [Cucumis melo var. makuwa])

HSP 1 Score: 295.0 bits (754), Expect = 4.0e-76
Identity = 147/148 (99.32%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR 82
           MGIVGFVSDYVTDN GSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR
Sbjct: 1   MGIVGFVSDYVTDNFGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR 60

Query: 83  KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP 142
           KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP
Sbjct: 61  KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP 120

Query: 143 QALPVDEALDQRLTMMFSDENPNACSIM 171
           QALPVDEALDQRLTMMFSDENPNACSIM
Sbjct: 121 QALPVDEALDQRLTMMFSDENPNACSIM 148

[Close]

BLAST of CSPI05G19710 vs. NCBI nr
Match: XP_023551464.1 (heavy metal-associated isoprenylated plant protein 22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 260.0 bits (663), Expect = 1.4e-65
Identity = 132/149 (88.59%), Postives = 139/149 (93.29%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSR-KKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVD 82
           MGI  FVSD++TDNLGSR KKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSV+V+
Sbjct: 1   MGIADFVSDWLTDNLGSRKKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVEVN 60

Query: 83  RKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKA 142
           RKQSKVT  GYAE +KVLKKV+STGKKAELWPYVPY+ VAYPYV QAYDKKAPPGYVKKA
Sbjct: 61  RKQSKVTAVGYAEPSKVLKKVQSTGKKAELWPYVPYSVVAYPYVAQAYDKKAPPGYVKKA 120

Query: 143 PQALPVDEALDQRLTMMFSDENPNACSIM 171
           PQA PVDEA DQRLTMMFSDENPNACSIM
Sbjct: 121 PQAFPVDEAPDQRLTMMFSDENPNACSIM 149

[Close]

BLAST of CSPI05G19710 vs. NCBI nr
Match: XP_022939481.1 (heavy metal-associated isoprenylated plant protein 22-like [Cucurbita moschata])

HSP 1 Score: 257.7 bits (657), Expect = 7.1e-65
Identity = 131/149 (87.92%), Postives = 138/149 (92.62%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSR-KKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVD 82
           MGI  FVSD++ DNLGSR KKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSV+V+
Sbjct: 1   MGIADFVSDWLIDNLGSRKKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVEVN 60

Query: 83  RKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKA 142
           RKQSKVT  GYAE +KVLKKV+STGKKAELWPYVPY+ VAYPYV QAYDKKAPPGYVKKA
Sbjct: 61  RKQSKVTAVGYAEPSKVLKKVQSTGKKAELWPYVPYSVVAYPYVAQAYDKKAPPGYVKKA 120

Query: 143 PQALPVDEALDQRLTMMFSDENPNACSIM 171
           PQA PVDEA DQRLTMMFSDENPNACSIM
Sbjct: 121 PQAFPVDEAPDQRLTMMFSDENPNACSIM 149

[Close]

BLAST of CSPI05G19710 vs. NCBI nr
Match: KAG6579239.1 (Heavy metal-associated isoprenylated plant protein 22, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016754.1 Heavy metal-associated isoprenylated plant protein 22 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 256.1 bits (653), Expect = 2.1e-64
Identity = 130/149 (87.25%), Postives = 137/149 (91.95%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSR-KKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVD 82
           MGI  FVSD++ DNLGSR KKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSV+V+
Sbjct: 1   MGIADFVSDWLIDNLGSRKKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVEVN 60

Query: 83  RKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKA 142
           RKQSKVT  GYAE +KVLKKV+STGKKAELWPYVPY+ VAYPYV QAYDKKAPPGYVKK 
Sbjct: 61  RKQSKVTAVGYAEPSKVLKKVQSTGKKAELWPYVPYSVVAYPYVAQAYDKKAPPGYVKKV 120

Query: 143 PQALPVDEALDQRLTMMFSDENPNACSIM 171
           PQA PVDEA DQRLTMMFSDENPNACSIM
Sbjct: 121 PQAFPVDEAPDQRLTMMFSDENPNACSIM 149

[Close]

BLAST of CSPI05G19710 vs. TAIR 10
Match: AT1G22990.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 4.6e-46
Identity = 91/152 (59.87%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLG---SRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVK 82
           MG + ++S+Y +++      ++KKRK  QTV IKVK+DCDGCER+IKNAVSS+KG KSV+
Sbjct: 1   MGALNYLSEYFSNHFYVSIRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVE 60

Query: 83  VDRKQSKVTVNGYAEATKVLKKVESTG-KKAELWPYVPYNSVAYPYVPQAYDKKAPPGYV 142
           V+RK  KVTV+GY +  KVLK V+STG KKAELWPYVPY  VAYPY   AYDK+APPG+V
Sbjct: 61  VNRKMHKVTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFV 120

Query: 143 KKAPQALPVDEALDQRLTMMFSDENPNACSIM 171
           +K+ QA     + D +L  +FSDENPNAC++M
Sbjct: 121 RKSEQAQAQPGSTDDKLMSLFSDENPNACTVM 152

[Close]

BLAST of CSPI05G19710 vs. TAIR 10
Match: AT1G71050.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 1.4e-45
Identity = 93/154 (60.39%), Postives = 117/154 (75.97%), Query Frame = 0


Query: 23  MGIVGFVSDYVTD--NLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKV 82
           MG +  +S+Y++D   +  +++KRK  QTV IKVKMDCDGCERR+KNAVSS+KGVKSV+V
Sbjct: 1   MGALDSLSEYISDYFRVTRKRRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEV 60

Query: 83  DRKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKK 142
           +RK  KVTV+GY E  KVLK++E TGKKAE+WPYVPYN VAYPY    YDKKAP GYV+K
Sbjct: 61  NRKIHKVTVSGYVEPKKVLKRIERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVRK 120

Query: 143 APQA----LPVDEALDQRLTMMFSDENPNACSIM 171
           + Q+    LP   A +     +FSDENPNAC++M
Sbjct: 121 SEQSQLQLLP--GAPENHYISLFSDENPNACTVM 152

[Close]

BLAST of CSPI05G19710 vs. TAIR 10
Match: AT4G39700.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 176.0 bits (445), Expect = 2.5e-44
Identity = 89/156 (57.05%), Postives = 120/156 (76.92%), Query Frame = 0


Query: 26  VGFVSDYVTDNLGS-------RKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSV 85
           VG   +Y+++ +G+       ++KK+K  QTVE+KV+MDCDGC  +IKN++SS+KGVK+V
Sbjct: 3   VGGTLEYISELIGNGGSHSYGKRKKKKQFQTVELKVRMDCDGCVLKIKNSLSSLKGVKTV 62

Query: 86  KVDRKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYV 145
           ++++KQ KVTV+GYA+A+KVLKK ++TGKKAE+WPYVPYN VA PY+ QAYDKKAPPGYV
Sbjct: 63  EINKKQQKVTVSGYADASKVLKKAKATGKKAEIWPYVPYNLVAQPYIAQAYDKKAPPGYV 122

Query: 146 KKA-PQALPVDEAL---DQRLTMMFSDENPNACSIM 171
           +K  P       A+   D   T +FSD+NPNACSIM
Sbjct: 123 RKVDPNVTTGTMAVYYDDPSYTSLFSDDNPNACSIM 158

[Close]

BLAST of CSPI05G19710 vs. TAIR 10
Match: AT5G17450.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 167.9 bits (424), Expect = 6.9e-42
Identity = 79/149 (53.02%), Postives = 109/149 (73.15%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSRKKKRKPNQTVEIKVKMDCDGCERRIKNAVSSVKGVKSVKVDR 82
           MG   ++S + +    + K KRKP QTV+IKVKMDCDGCERR++N V  +KGVKSV+V+R
Sbjct: 1   MGAFDYISSFCSYTYANAKTKRKPLQTVDIKVKMDCDGCERRVRNVVRRMKGVKSVEVNR 60

Query: 83  KQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKKAP 142
           KQS++TVNG+ +  KVLK+V+STGKKAE WPY+P + V YP+ P  YDK+AP G+++   
Sbjct: 61  KQSRITVNGHVDPNKVLKRVKSTGKKAEFWPYIPQHMVYYPFAPGMYDKRAPAGHIRNPT 120

Query: 143 QALPVDEALDQRLTMMFSDENPN-ACSIM 171
           Q+ P   A ++    +FSD+N + ACSIM
Sbjct: 121 QSFPTANAPEENYVSLFSDDNVHAACSIM 149

[Close]

BLAST of CSPI05G19710 vs. TAIR 10
Match: AT4G08570.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 6.5e-40
Identity = 86/151 (56.95%), Postives = 109/151 (72.19%), Query Frame = 0


Query: 23  MGIVGFVSDYVTDNLGSRK-KKRKPNQTVEIKV-KMDCDGCERRIKNAVSSVKGVKSVKV 82
           MG+ G   +Y++D L  RK KK+K  QTV ++V ++DC+GCER+IK+ +S VKGVKSV V
Sbjct: 1   MGVEG-TMEYISDLLKKRKRKKKKQMQTVALRVARIDCEGCERKIKHVLSGVKGVKSVDV 60

Query: 83  DRKQSKVTVNGYAEATKVLKKVESTGKKAELWPYVPYNSVAYPYVPQAYDKKAPPGYVKK 142
           D K  KVTV GY +  KVL+  +ST KK ELWPYVPY  VA PY+ QAYDKKAPP  V+K
Sbjct: 61  DVKLQKVTVTGYIDPKKVLEAAKSTKKKVELWPYVPYTMVANPYISQAYDKKAPPNMVRK 120

Query: 143 APQALPVDE-ALDQRLTMMFSDENPNACSIM 171
            P    V+E  +D   T+MFSDENPN+C+IM
Sbjct: 121 VPDTASVNETTVDDSYTIMFSDENPNSCAIM 150

The following BLAST results are available for this feature:

BLAST of CSPI05G19710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VP2	6.5e-45	59.87	Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana OX...	[more]
Q9C9A3	1.9e-44	60.39	Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana OX...	[more]
O65657	3.6e-43	57.05	Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX...	[more]
Q9LF57	9.8e-41	53.02	Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana OX...	[more]
O81464	9.1e-39	56.95	Heavy metal-associated isoprenylated plant protein 24 OS=Arabidopsis thaliana OX...	[more]

BLAST of CSPI05G19710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPL8	6.7e-77	100.00	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G571510 PE=4 SV...	[more]
A0A5D3E3U3	2.0e-76	99.32	Heavy metal-associated isoprenylated plant protein 22 OS=Cucumis melo var. makuw...	[more]
A0A1S3CSV0	2.0e-76	99.32	heavy metal-associated isoprenylated plant protein 22 OS=Cucumis melo OX=3656 GN...	[more]
A0A6J1FG16	3.5e-65	87.92	heavy metal-associated isoprenylated plant protein 22-like OS=Cucurbita moschata...	[more]
A0A6J1JVQ2	5.0e-64	87.25	heavy metal-associated isoprenylated plant protein 22-like OS=Cucurbita maxima O...	[more]

BLAST of CSPI05G19710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150477.2	5.8e-91	100.00	heavy metal-associated isoprenylated plant protein 22 [Cucumis sativus] >KGN5209...	[more]
XP_008467147.1	4.0e-76	99.32	PREDICTED: heavy metal-associated isoprenylated plant protein 22 [Cucumis melo] ...	[more]
XP_023551464.1	1.4e-65	88.59	heavy metal-associated isoprenylated plant protein 22-like [Cucurbita pepo subsp...	[more]
XP_022939481.1	7.1e-65	87.92	heavy metal-associated isoprenylated plant protein 22-like [Cucurbita moschata]	[more]
KAG6579239.1	2.1e-64	87.25	Heavy metal-associated isoprenylated plant protein 22, partial [Cucurbita argyro...	[more]

BLAST of CSPI05G19710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G22990.1	4.6e-46	59.87	Heavy metal transport/detoxification superfamily protein	[more]
AT1G71050.1	1.4e-45	60.39	Heavy metal transport/detoxification superfamily protein	[more]
AT4G39700.1	2.5e-44	57.05	Heavy metal transport/detoxification superfamily protein	[more]
AT5G17450.1	6.9e-42	53.02	Heavy metal transport/detoxification superfamily protein	[more]
AT4G08570.1	6.5e-40	56.95	Heavy metal transport/detoxification superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 53..108 e-value: 7.4E-15 score: 55.1
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 56..111 score: 12.311873
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 51..110 e-value: 3.51065E-15 score: 64.5493
None	No IPR available	GENE3D	3.30.70.100		coord: 35..113 e-value: 7.4E-21 score: 76.1
None	No IPR available	PANTHER	PTHR22814:SF324	SUBFAMILY NOT NAMED	coord: 29..170
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 29..170
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 48..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G19710.1	CSPI05G19710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding