CSPI05G19200 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G19200
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMACPF domain-containing protein
LocationChr5: 20456298 .. 20462590 (+)
RNA-Seq ExpressionCSPI05G19200
SyntenyCSPI05G19200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCAAAATTTCCAAATTTCCACACGAAGATGGAGATAGACAGAGTATCCATATCCGACTTCATCATATCAAATTAAACTTTTCTTTAAAAGATAAAATAAATCGGAAAGAAGAAACTTCCCAAATCCCAATTCCAAATTTCTTTTTCTCGTTCTCTCTTCAAAATTTCATCATTCTTTCAACCAAACAGTTGCACTCACCTCCTTCCTTCCGCCATTAAAATTCCCCCCATCAATCTCCTTTATGCTACTAACAAACAGCTACACACAAAAATGGCAGATGAAATAGTAGAAAAAGCTCTCAACAGCTTAGGAAAAGGGTTCGATCTCACCTCCGATTTCCGCCTCAAATACTGTAAAGGCCAACGCAGTCTCGTTCTTCTCAACCAATCTCAGAGACGAGACCTAATCGTACCTGGTTTCGGCGCCGTCAAAGACGTCTCTATCGACATTAAATGTGATCGAGGTGATCGGACTCGTTACCAGTCCGATATTCTCAACTTCAATCAGGTAATAAAAACCTAATCGCCTTCCAAATTGAAATTTCTTCTTCACTTCTCTATTTTTCCTTTTTGAAATTTCGTTGCTATTATTCAGATGTCGGAACTTTTCAATCGGAAATGTTCGATTCCAGGCAAGATACCGTCCGGATCGTTTAATTCCATGTTCGGATTTCAGAGTGGATGTTGGGCGACGGATGCTGTGAATACCAAATGCTTAGGGCTTGATGGTTACTTCATCAGCCTCTTTAACGCTCACATTGATCGTTATCCACTTCGCCTATCCGATGAGGTTCGCTCCGCCGTGCCGTCGACTTGGGATCCCCCTGCTATTGCCAGGTAATTCCAATTCCTTAATTTAAAAGCTATTTCTTTTTTCTAAATAAAAAAATATCAGTTAATTTATAAATTTTAACGGTAAATTTAAAAATTAGAAATGGAGGATATAATTTTCACTTGGAAATACTTTATGCTTCAAAGATGATGTTAGTATCTTAATGGTTGAACATTTCAATTCTCCATTTTTATTTTTAAAAAATGTTTTATTGTTTTTTGTATTTGATCTCTTAAATTTAATATTGATTTCTCAAGCTAAAAGTGATGATTTGAATGAAGATAATGGGGGGGTAACTTTGGTTTAATTTTATATGGTAGTGGCTAAAGTTGAAGTTCAATGATGGTTCTTTTTTTATTTATGTCATAGTTGAAATGATGGTTAAAGGACTCTAAGGAGTGAATGTCTATATATATAATTTATTTCAAAAGTTGAACCATCAAAGGAAATGGTTAATGACTTTTGTTTTCAAACTTTTTAATTAGAATAAAAATCTAACAAAATAACTCATTAGAGACAATTGATCGAATTTACTTTTGAAGGGATAATAATTAGTAAAAACAGAAGTTGAAACGTGTCAACATTGTATTGGTGTAGCAAGGGCGGTAAAAGAGATAGATGCAGCCCTATACTCCAACTACACCTAAATTTTTGTCTTATTAAAAAGAAGTAAAATGATTATTGGCAAACTAAAATTTTAAATTCCATGATCACATATGTTTAATGTTTTTACAAAAAGCTAACCCAAAAGAGATATGCATATTCTATAGGCATCATTAGTAGCTATATCACTATAGATCCTAAAATTTTAAATATACTACTAAGAAGCACCATAAATATTTTAGATTCATTTTCTTCTAGATAAATATATTGAATCCTTTTTTGGAAAAGACATTGCTAAAATAACTTTTAATTCATGGAAAAGGAGGAGAACAACCTCATGTTACCTTCTTAAAAATGAACCTATTATAGCTAGTCAAATATATATTAGAAATACTAAAGTTAAATTGCTTTTAAACTTATGAACCAAGATAATATTTTAACCAATAGTATTAAATTGATGGTGTGACTTGTGAGATGTATGAAATATGATGAGGGAAATAGGTTGGGATTCTTGACTTGGGCAAGTGGGAAATTATTAGCTTAACAAATCTTTACCTATCAATGACAAGACAGCTATTGAGAAAGAGAATACGAAAGTTCATATTGCTCCAAAGATGTATTTATCAATTTCTTTTCTCTTTATTTTAACTTTAAATTCCATTTCAACCCATGAATTTTTTATAATATTCTAAAATAGACTTAAACAAATATCCTTTAAAAGTTTGGAGAGTGAAATACAATTATCTTTTATATTAGTTTAGAAACTAAAGTACAACCATCAACAAAATTTGATTATTTATTTTTTAAATAGATTAATGATATTCTTGTTAGAAGAAATCTGACCAGTTCATTACATTAGAGTTTTAAAATATTCTGTCATTTTTGCTCTCAATTATTTTTGTAGTATAATAAACCTTTACTCTATGGTCACCCTATTGGTTGTTCATGGAGTCTTCCATCTATATATGTCTTCATATACCAACTCTACTTAATATGTAATTAATTTTCCTGATGTAGATTTTTTTTAATTCAATAAATTTCATAACTATAATAAATAAATGAAAAAAAAATTCAAATTACCTATATTTATTGTTTGTCATGTTAACATACATGTCAAATAGTGTGAAATTCTACCGACATGGATAATGAAGTCAACTTGTATCTCTCTCTCTATCATTAAAGTAAAATTTTATTAATTTATTAACAAAATTCTATATTAACCCATAAATTGATAAATTGATTTTTTTTTTTAAAAAAAGGAAAAGTCCAAAAAATCAGCTTTATTTTATTTTTATTTACAACAACTTAAGATGGCTGAGTATTTGGTTATGCTAATAAACAAATCTCAAACCTTGATTGTAATAGATAGTCAACTCATCTAATTGCTGACACTATTTATTTATTTATTGACAATGACATAAGTGAGAGTATGGGTAGAGGGCTTCAAATATGAAGGTATATTTCAAATGAATTAAATATTTATATGTTGTTAACATGTTAGAGTCATCCTGAAATAGTTAGAATTAATTTAGTTCAAATAGACATTTTACACTTTTAAAAGTAATTTTCATATACTATACTATGAGTTGAATTGATTTCGATCCATTTTGACAACTCATTTTAGTCCTTTTGAAATAAGATCTAGTAATTGTTATTAAATGCTTCACAAATTTCAAACGTAGAAATTTGTTGGGCAGCCTCTTTTGAGGATCATTTCAGGGTCTTGATTGACTTTTAATTCTTTGTGTTCTAATTCACTTTTTCCACCTCTATATTAATAAACCATTTTTAAGATTGTTAAACTCAAAGATATAGAAAATTGGGTAATGGTTAGGTGTAGTCTAGACTATGTTTATATCTCATGCAACTTTTTTGTTACATAGTAAAAGAAAGTAATTCCATTCTTTAACAGTGAAATATTTGTCACATATGATCACAGGTGTAAACAATCTATGTATTATTCTAACCTGAACCAATCTTTAATGTATTATAATGTTCAAAATTGATAGATGCTAACTATTAGATTTTGTTTCCGAAACATAAATTTCCATATGATCATGAAATAATATGAACACTCAATATTGTCTTAATATACTTTACTACAAAGTTGTGTTTTGTCAAACTCAAGAAAGAAAGACAAAAATATAGACTTATATTTTGTCTCTGAGTCAATGGCTTCCAGGAAATTAGAGAGGGGAAAAAATGGATTGGACTTTCCAAAGTTGCACAGAAGAAAAATGGAATTTTTTACATTTTAATCTTTGTTCCTTTGGACTATGTACTGAAATTTTATCGTCTAAGATACTCTTCTCATTCTCCATAATTTTTTAAAATACATGTGGTGGGTTTGCCTGTAATCTCAACTAGGGTGTAAGAAACCGATGAACCAAACCAAACTAATAGGAAAAAAAACTGAAGTTTAAATAAAGGGGGTATATAATTATGGAACAGGTTCATAGAGAAGTATGGAACTCATATAATAGTTGGGCTGAGCATTGGAGGACGTGATGTGGTTCTTGTAAGGCAAGACAAATCATCCAAATTGGAACCATCAGAGCTCAAAAACCACTTGGAAGAGTTGGGCGATCAGCTTTTCAATGGAGCTTGCACTTTTCCTCCACCAGTTTCTAAACCTAAAGATCATCACAAACCCAAGGTCTAAATGATATACTTCTCATTTCTTTCATATATCATACAATTTCTTAAGCTTTATTTGTGTTTGGAATTGTTATTTCTCTTATATGAACGAAGTAAATTTTAGATGGTTAATGTCGTTTGACCTAAATTGGATAATCGGCCTCATTACCATTATTATTGTAGTGCACTAACGTGTTTAAAATTATTTATTCTACCTTTAGATTTTATATGGGTAACGAGGGGTTTAGAGTAGTGATTAAGCTCAAGCAAGAGGGAGGAAGAGTCCTAGCTTGTGAGAGGACTAGTAAAGGAATGAGAAACGTGAGGGAGGGAGGGAGAGCACAATTTTGTACATATGATCCTTAGTTTCGCACTTGATAAAAATCGTGAGGAGCAATATAAATAATCATTACGCTTTTAGGTGCACTCTGAGCACTCTTACAATAGTAAAGGGTCATCTTTCATTACTTAGCAATGAGGTAAATGAACAAGTTATTTAAAAAGCTTATTTGAAATGACTTTTTAACTACTTAAAAAGCTTATTTGAAATGACTTTTTAACTACTTAAAGAAACAAGTGCTTTAAACATTTAGAACATCATTCCAAAGTACTACAATACTTCATAGAATTATTGTACAAATCTAAACTTGTACGTTTTCTCACATGGATGCAGATACCACAAGCTTTCAACTTGTTTGATCCCCAACTTGTGGCTTTTGGCAACTTCTCTTCTGTCAGTGCAAAAGACGTAAGTTCAAGAAAACCAAAGTCTATAATATAATTAAAAATCTTCTATTCAACTCTTTATGATTTTTGAGATTAGTCTTTTTGCTTGTTTTCTGGTAGGGAATCATAGTGATCACTTCAAAGAGAGGAGGAAGTCCAACAGCAAGTAGTCATTGTGAGTGGCTACCGACTGTCCACGAATCGCCCGATGCAATTCATTTTCAGTTCATACCGATCATCTCTCTTCTCGAATGCATTCCGGGCAAAGGATTTCTTTCCCATGCCATTAACCTTTATCTCCGATGTACGTATATGCAATCTTTCTTCTTCATCACAGTCCTCTCGTGTTGCTTCTCTTGATCTTATATGTGTTTTTCTCTTGTAGACAAACCTCCTATAGCAGACTTGCAATACTTCTTGGACTTTCAAGTGCACAAGATTTGGGCACCCATCCATAATGATCTTCCTTTAGGACCAACTCCCAATATTTCTACTTCTCCTTCCCTTCAACTCAACTTGATGGGTCCAAAACTATATGTAAATACAACTCAGGTAAACCCTAACCACTCATGTACTTAACTTTCAAGTCTAGCTTGTGGCGACTTCTTCAAACCTGTGATTTGAAGTATGCAAAGATATTTATAGGTTATAGCAGATAACAAACCAGTTACAGGAATGAGATTATATCTTGAAGGCATGAAATCAAACAGGTAACAAATGTAATAAATTCTTTAGTATAATAACTGCTGTGAATTACCGTTGAGCTAAATTTAAAGTTTGTATTTGTAGGTTGGCCATTCACATCCAACACCTTCTAACTACTCCTCTTCTTCTTGAAAACAACATCACTCAACAAACTTACTGGCATTCTTCACAGCACATCGCGGACGACCGTTATGTCGAGCCACTAAATCGCAACAAATTCTCACATGTATGTACAATTCCAATCAAGTACGACCCTGCTTGGGCTACCACTTCCGGTGATGTCAACTCCCCAGCAGCGTTCATAGTCACCGGAGCCCAGCTCCTTGTTGTAAAACAACACGACTCAAAAACCGTCCTCCATCTTGGCCTCCTCTATTCTAGGGTCCCTAACTCGTATATTGTCCAAACCAATTGGGCCCGCCACTCCTCCGACATCTCCCCAAAGTCTGGTATCTTCTCAGCGATCAGCACCTCCTTAACTGGTACCTCGGCTGCCCTCAAAGAGAAGCCACCAGAGGCTGCTGTGATAGTGGATTCGGGAGTTTTTCATGGTGGGCCTCCGATGTCGGTGCAGGTGCAGAGGCTTGTGAAGTTCGTGGATTTGTCGGAGATGCGTAAGGGGCCACAGGACAGTCCGGGACATTGGATGGTCATTGGAGCTAGGTTGAACTTGGAGAAAGGGAAGATTTGTTTACAAGTTAAGTTCGGTTTGTTACATGTAGTTTCAGAAACTATAACTGATGATCAATGAACTTCAAGAAACAAGAAACAATATATTGGTAAGTTCTTT

mRNA sequence

GTCAAAATTTCCAAATTTCCACACGAAGATGGAGATAGACAGAGTATCCATATCCGACTTCATCATATCAAATTAAACTTTTCTTTAAAAGATAAAATAAATCGGAAAGAAGAAACTTCCCAAATCCCAATTCCAAATTTCTTTTTCTCGTTCTCTCTTCAAAATTTCATCATTCTTTCAACCAAACAGTTGCACTCACCTCCTTCCTTCCGCCATTAAAATTCCCCCCATCAATCTCCTTTATGCTACTAACAAACAGCTACACACAAAAATGGCAGATGAAATAGTAGAAAAAGCTCTCAACAGCTTAGGAAAAGGGTTCGATCTCACCTCCGATTTCCGCCTCAAATACTGTAAAGGCCAACGCAGTCTCGTTCTTCTCAACCAATCTCAGAGACGAGACCTAATCGTACCTGGTTTCGGCGCCGTCAAAGACGTCTCTATCGACATTAAATGTGATCGAGGTGATCGGACTCGTTACCAGTCCGATATTCTCAACTTCAATCAGATGTCGGAACTTTTCAATCGGAAATGTTCGATTCCAGGCAAGATACCGTCCGGATCGTTTAATTCCATGTTCGGATTTCAGAGTGGATGTTGGGCGACGGATGCTGTGAATACCAAATGCTTAGGGCTTGATGGTTACTTCATCAGCCTCTTTAACGCTCACATTGATCGTTATCCACTTCGCCTATCCGATGAGGTTCGCTCCGCCGTGCCGTCGACTTGGGATCCCCCTGCTATTGCCAGGTTCATAGAGAAGTATGGAACTCATATAATAGTTGGGCTGAGCATTGGAGGACGTGATGTGGTTCTTGTAAGGCAAGACAAATCATCCAAATTGGAACCATCAGAGCTCAAAAACCACTTGGAAGAGTTGGGCGATCAGCTTTTCAATGGAGCTTGCACTTTTCCTCCACCAGTTTCTAAACCTAAAGATCATCACAAACCCAAGATACCACAAGCTTTCAACTTGTTTGATCCCCAACTTGTGGCTTTTGGCAACTTCTCTTCTGTCAGTGCAAAAGACGGAATCATAGTGATCACTTCAAAGAGAGGAGGAAGTCCAACAGCAAGTAGTCATTGTGAGTGGCTACCGACTGTCCACGAATCGCCCGATGCAATTCATTTTCAGTTCATACCGATCATCTCTCTTCTCGAATGCATTCCGGGCAAAGGATTTCTTTCCCATGCCATTAACCTTTATCTCCGATACAAACCTCCTATAGCAGACTTGCAATACTTCTTGGACTTTCAAGTGCACAAGATTTGGGCACCCATCCATAATGATCTTCCTTTAGGACCAACTCCCAATATTTCTACTTCTCCTTCCCTTCAACTCAACTTGATGGGTCCAAAACTATATGTAAATACAACTCAGGTTATAGCAGATAACAAACCAGTTACAGGAATGAGATTATATCTTGAAGGCATGAAATCAAACAGGTTGGCCATTCACATCCAACACCTTCTAACTACTCCTCTTCTTCTTGAAAACAACATCACTCAACAAACTTACTGGCATTCTTCACAGCACATCGCGGACGACCGTTATGTCGAGCCACTAAATCGCAACAAATTCTCACATGTATGTACAATTCCAATCAAGTACGACCCTGCTTGGGCTACCACTTCCGGTGATGTCAACTCCCCAGCAGCGTTCATAGTCACCGGAGCCCAGCTCCTTGTTGTAAAACAACACGACTCAAAAACCGTCCTCCATCTTGGCCTCCTCTATTCTAGGGTCCCTAACTCGTATATTGTCCAAACCAATTGGGCCCGCCACTCCTCCGACATCTCCCCAAAGTCTGGTATCTTCTCAGCGATCAGCACCTCCTTAACTGGTACCTCGGCTGCCCTCAAAGAGAAGCCACCAGAGGCTGCTGTGATAGTGGATTCGGGAGTTTTTCATGGTGGGCCTCCGATGTCGGTGCAGGTGCAGAGGCTTGTGAAGTTCGTGGATTTGTCGGAGATGCGTAAGGGGCCACAGGACAGTCCGGGACATTGGATGGTCATTGGAGCTAGGTTGAACTTGGAGAAAGGGAAGATTTGTTTACAAGTTAAGTTCGGTTTGTTACATGTAGTTTCAGAAACTATAACTGATGATCAATGAACTTCAAGAAACAAGAAACAATATATTGGTAAGTTCTTT

Coding sequence (CDS)

ATGGCAGATGAAATAGTAGAAAAAGCTCTCAACAGCTTAGGAAAAGGGTTCGATCTCACCTCCGATTTCCGCCTCAAATACTGTAAAGGCCAACGCAGTCTCGTTCTTCTCAACCAATCTCAGAGACGAGACCTAATCGTACCTGGTTTCGGCGCCGTCAAAGACGTCTCTATCGACATTAAATGTGATCGAGGTGATCGGACTCGTTACCAGTCCGATATTCTCAACTTCAATCAGATGTCGGAACTTTTCAATCGGAAATGTTCGATTCCAGGCAAGATACCGTCCGGATCGTTTAATTCCATGTTCGGATTTCAGAGTGGATGTTGGGCGACGGATGCTGTGAATACCAAATGCTTAGGGCTTGATGGTTACTTCATCAGCCTCTTTAACGCTCACATTGATCGTTATCCACTTCGCCTATCCGATGAGGTTCGCTCCGCCGTGCCGTCGACTTGGGATCCCCCTGCTATTGCCAGGTTCATAGAGAAGTATGGAACTCATATAATAGTTGGGCTGAGCATTGGAGGACGTGATGTGGTTCTTGTAAGGCAAGACAAATCATCCAAATTGGAACCATCAGAGCTCAAAAACCACTTGGAAGAGTTGGGCGATCAGCTTTTCAATGGAGCTTGCACTTTTCCTCCACCAGTTTCTAAACCTAAAGATCATCACAAACCCAAGATACCACAAGCTTTCAACTTGTTTGATCCCCAACTTGTGGCTTTTGGCAACTTCTCTTCTGTCAGTGCAAAAGACGGAATCATAGTGATCACTTCAAAGAGAGGAGGAAGTCCAACAGCAAGTAGTCATTGTGAGTGGCTACCGACTGTCCACGAATCGCCCGATGCAATTCATTTTCAGTTCATACCGATCATCTCTCTTCTCGAATGCATTCCGGGCAAAGGATTTCTTTCCCATGCCATTAACCTTTATCTCCGATACAAACCTCCTATAGCAGACTTGCAATACTTCTTGGACTTTCAAGTGCACAAGATTTGGGCACCCATCCATAATGATCTTCCTTTAGGACCAACTCCCAATATTTCTACTTCTCCTTCCCTTCAACTCAACTTGATGGGTCCAAAACTATATGTAAATACAACTCAGGTTATAGCAGATAACAAACCAGTTACAGGAATGAGATTATATCTTGAAGGCATGAAATCAAACAGGTTGGCCATTCACATCCAACACCTTCTAACTACTCCTCTTCTTCTTGAAAACAACATCACTCAACAAACTTACTGGCATTCTTCACAGCACATCGCGGACGACCGTTATGTCGAGCCACTAAATCGCAACAAATTCTCACATGTATGTACAATTCCAATCAAGTACGACCCTGCTTGGGCTACCACTTCCGGTGATGTCAACTCCCCAGCAGCGTTCATAGTCACCGGAGCCCAGCTCCTTGTTGTAAAACAACACGACTCAAAAACCGTCCTCCATCTTGGCCTCCTCTATTCTAGGGTCCCTAACTCGTATATTGTCCAAACCAATTGGGCCCGCCACTCCTCCGACATCTCCCCAAAGTCTGGTATCTTCTCAGCGATCAGCACCTCCTTAACTGGTACCTCGGCTGCCCTCAAAGAGAAGCCACCAGAGGCTGCTGTGATAGTGGATTCGGGAGTTTTTCATGGTGGGCCTCCGATGTCGGTGCAGGTGCAGAGGCTTGTGAAGTTCGTGGATTTGTCGGAGATGCGTAAGGGGCCACAGGACAGTCCGGGACATTGGATGGTCATTGGAGCTAGGTTGAACTTGGAGAAAGGGAAGATTTGTTTACAAGTTAAGTTCGGTTTGTTACATGTAGTTTCAGAAACTATAACTGATGATCAATGA

Protein sequence

MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCLGLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQLVAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLMGPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSSQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFGLLHVVSETITDDQ*
Homology
BLAST of CSPI05G19200 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 2.6e-200
Identity = 350/616 (56.82%), Postives = 447/616 (72.56%), Query Frame = 0

Query: 4   EIVEKALNSLGKGFDLTSDFRLKYCK------GQRSLVLLNQSQRRDLIVPGFGAVKDVS 63
           +++E A+ SLGKGFDLT+DFRLKYCK      G   LV+L+Q+Q R+L +PGFG  ++VS
Sbjct: 7   DVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVS 66

Query: 64  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 123
            DI CD+G+RTR++SDIL+FN+MSE FN++ S+ GKIPSG+FN+ FGFQSG WATDA N 
Sbjct: 67  ADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANV 126

Query: 124 KCLGLDGYFISLFNAHI-DRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIG 183
           K LGLD   ++LFN HI +   LRL+D VR+AVPS+WDP  +ARFIE+YGTH+I G+S+G
Sbjct: 127 KSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVG 186

Query: 184 GRDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHH---KPKIPQAF 243
           G+DVV+VRQDKSS L+   L++HL +LGDQLF G+C           HH   +PK P+AF
Sbjct: 187 GQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF 246

Query: 244 NLF-DPQLVAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPI 303
           N+F D Q VAF NF S+++++GI VI +KRGG   A SH EWL TV + PDAI+F FIPI
Sbjct: 247 NVFDDKQTVAFNNF-SINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPI 306

Query: 304 ISLLECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTS 363
            SLL+ +PG G LSHA++LYLRYKPP+ DLQYFLDF   + WAP+HNDLP G  PN++++
Sbjct: 307 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASA 366

Query: 364 -PSLQLNLMGPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNI 423
            P+L +N MGPKLYVNTT V ++  PVTGMR +LEG K NRLAIH+QHL  T   +   I
Sbjct: 367 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 426

Query: 424 TQQTYWHSSQHIAD-DRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNS--PAAFIVTG 483
           T +  W  S  I D DRY EPLN  KFSHVCT+P+KYDP W  T+ +  S    AFIVTG
Sbjct: 427 TDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 486

Query: 484 AQLLVVKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSA 543
           AQ L VK+H SK+VLHL L Y++V + Y+VQ +W       S KSGIFS++S  LT  S 
Sbjct: 487 AQ-LEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSV 546

Query: 544 ALKE-KPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLN 603
                +  +  V++DSGVF GGPP+     ++VKFVDLS++ +GPQ SPGHW+V G RL 
Sbjct: 547 HHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLY 606

BLAST of CSPI05G19200 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 1.0e-140
Identity = 272/607 (44.81%), Postives = 379/607 (62.44%), Query Frame = 0

Query: 2   ADEIVEKALNSLGKGFDLTSDFRLKYCKG----QRSLVLLNQSQRRDLIVPGFGAVKDVS 61
           A +  E A+ S+G G+DL  D RLKYCKG     R L +       ++++PG  ++ +VS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 62  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 121
             IKCD+G+R R++SDIL F QM+E FN++ S+ GKIPSG FN+MF F S CW  DA  T
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYT 126

Query: 122 KCLGLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG 181
           K L  DG FISL++  +D+  + L + V+ AVPSTWDP A+ARFI+ YGTHIIV + +GG
Sbjct: 127 KNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGG 186

Query: 182 RDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFD 241
           +DV+  +Q  SSKL+P +L+  L+E+ D+ F  A       S+          +   L  
Sbjct: 187 KDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRF 246

Query: 242 PQLVAFGNFSSVSAKDGIIVITSKRGGSPTAS-SHCEWLPTVHESPDAIHFQFIPIISLL 301
               + G++++   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL
Sbjct: 247 ADTSSLGSYAN---KEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLL 306

Query: 302 ECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQ 361
             +PG GFLSHAINLYLRYKPPI +L  FL+FQ+ + WAP+ ++LPLGP     +  SLQ
Sbjct: 307 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQ 366

Query: 362 LNLMGPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLL--LENNITQQ 421
            +  GPKLYVNTT V    +P+TGMRLYLEG +SNRLAIH+QHL + P +  LE+++ + 
Sbjct: 367 FSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRS 426

Query: 422 TYWHSSQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVV 481
                 Q   D RY E +N   +SHVCT P++ D        D++     +VTGAQ L V
Sbjct: 427 I----RQESHDRRYYEKVNWKNYSHVCTEPVESD-------DDLS-----VVTGAQ-LHV 486

Query: 482 KQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKP 541
           + H  K VL L L +SRV  + +V+ +    +   +PKSG+ S + +     +AA K  P
Sbjct: 487 ESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISH--HFTAAQKPPP 546

Query: 542 PEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKIC 601
             A V ++S ++ GGPP+  Q  +L+KFVD SEM +GPQ+SPG+W+V GARL +EKGKI 
Sbjct: 547 RPADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKIS 590

BLAST of CSPI05G19200 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 7.1e-134
Identity = 271/616 (43.99%), Postives = 377/616 (61.20%), Query Frame = 0

Query: 2   ADEIVEKALNSLGKGFDLTSDFRLKYCK----GQRSLVLLNQSQRRDLIVPGFGAVKDVS 61
           A    EKA++ +G G+DL SD R   CK    G R LV ++ ++ RDLI PG   V +VS
Sbjct: 11  AHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSR-LVEIDPTRNRDLIFPGGIVVNNVS 70

Query: 62  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 121
             IKCD+G+RTR +SDIL+FNQMSE FN+   + GKIPSG FN+MF F S CW  DA + 
Sbjct: 71  SSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAF-SKCWPKDASSV 130

Query: 122 KCLGLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG 181
           K L  DG+FISL++  I R  L L DEV+  VPS+WD  A+A FIEKYGTH++VG+++GG
Sbjct: 131 KTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGG 190

Query: 182 RDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFD 241
           +DV+ V+Q + S  EP E++  L+  GD+ F   C  P     P   +  K P+  NL  
Sbjct: 191 KDVIHVKQMRKSNHEPEEIQKMLKHWGDERF---CVDPVESKSPASVYSGK-PKEENLLQ 250

Query: 242 PQLVAFGNFSSVSA-----KDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPI 301
             L  FG   S +       + I+ +  +RGG     SH  WL TV ++P+ I   F+PI
Sbjct: 251 WGLQPFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPI 310

Query: 302 ISLLECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTS 361
            SLL  +PG GFLSHA+NLYLRYKPPI +L  FL+FQ+ + WAP++ DLPLG   +  +S
Sbjct: 311 TSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSS 370

Query: 362 PSLQLNLMGPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNIT 421
           PSLQ +LMGPKLYVNT++V +  +PVTG+R +LEG K N LAIH+QHL   P  L  +++
Sbjct: 371 PSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSL--HLS 430

Query: 422 QQTYWHSSQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLL 481
               +   +   +  Y  P+    FSHVCT P++Y       +G  +   A IVT A  L
Sbjct: 431 HDDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQY-------NGARSDDTASIVTKA-WL 490

Query: 482 VVKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSL-TGTSAALK 541
            VK    + VL L L +S   ++   ++ W   S++ S KSG+FS IST L TG S    
Sbjct: 491 EVKGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTN-SRKSGVFSMISTRLSTGLSPNPA 550

Query: 542 EKPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKG 601
              P++ + ++S V+  GP   V+  +L+  VD  E+ +GP++ PG+W+V GA+L +E G
Sbjct: 551 TTKPQSKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAG 608

Query: 602 KICLQVKFGLLHVVSE 608
           KI ++ K+ LL V+SE
Sbjct: 611 KISIKAKYSLLTVISE 608

BLAST of CSPI05G19200 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.8e-114
Identity = 247/611 (40.43%), Postives = 339/611 (55.48%), Query Frame = 0

Query: 6   VEKALNSLGKGFDLTSDFRLKYCKGQ--RSLVLLNQSQRRDLIVPGFGAVKDVSIDIKCD 65
           +  A+ +LG+GFD+TSD RL YCKG     LV + + Q RDL +     + +V  DI C 
Sbjct: 21  LRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCS 80

Query: 66  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCLGLD 125
           RG+    +  + +F++M+E FN +  + G IP G FN+MF + +G W  DA +TK L L 
Sbjct: 81  RGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNY-TGSWQVDAASTKSLALV 140

Query: 126 GYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDVVLV 185
           GYFI L++  + +  L L +E+R AVPS+WDP ++A FIE YGTHI+  ++IGGRDVV +
Sbjct: 141 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 200

Query: 186 RQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQLVAF 245
           RQ +SS L  SE++N++ ++                         I   F+  + Q +  
Sbjct: 201 RQHQSSPLPVSEIENYVNDM-------------------------IKHRFHEAESQSIT- 260

Query: 246 GNFSSVSAKD-GIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIPGK 305
                +  KD  I VI  +RGG     SH  W  TV  +PD I+  F PI+SLLE +PG 
Sbjct: 261 ---GPLKYKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGL 320

Query: 306 GFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLMGP 365
             L+ AI LYL YKPPI DLQYFLD+Q+ + WAP  ++L            SLQ +LMGP
Sbjct: 321 RHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGP 380

Query: 366 KLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSSQH 425
           KL+++  QV    KPVTG+RL LEG K NRL+IH+QHL++ P +L      Q +W S   
Sbjct: 381 KLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKIL------QPHWDSHVP 440

Query: 426 IA----------DDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLL 485
           I           D R+ EP+    FSHV T PI++     T  GD++     IVTGAQ L
Sbjct: 441 IGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHT---ETHIGDLS--GVHIVTGAQ-L 500

Query: 486 VVKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKE 545
            V    SK VLHL LL+S+VP   I ++ W     D +P              +S  L+ 
Sbjct: 501 GVWDFGSKNVLHLKLLFSKVPGCTIRRSVW-----DHTP------------VASSGRLEP 560

Query: 546 KPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGK 604
             P  +   +         +S Q  +L K VD SEM KGPQD PGHW+V GA+L +EKGK
Sbjct: 561 GGPSTSSSTEE--------VSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGK 560

BLAST of CSPI05G19200 vs. ExPASy TrEMBL
Match: A0A0A0KRG0 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G551250 PE=4 SV=1)

HSP 1 Score: 1261.5 bits (3263), Expect = 0.0e+00
Identity = 612/613 (99.84%), Postives = 613/613 (100.00%), Query Frame = 0

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI
Sbjct: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADN+PVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI
Sbjct: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600
           VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600

Query: 601 LLHVVSETITDDQ 614
           LLHVVSETITDDQ
Sbjct: 601 LLHVVSETITDDQ 613

BLAST of CSPI05G19200 vs. ExPASy TrEMBL
Match: A0A5D3CRV5 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold663G00330 PE=4 SV=1)

HSP 1 Score: 1226.8 bits (3173), Expect = 0.0e+00
Identity = 594/613 (96.90%), Postives = 604/613 (98.53%), Query Frame = 0

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIV+ ALNSLGKGFDLTSDFRLKYCKG  SLVLLNQSQRRDLIVPGFGAVKDVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP+SKPKDHHKPKIPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE IP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADNKPVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCT+PIKYDPAWATTSGD NSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHL LLYS+V NSYIVQTNWARHSSDISPKSGIFSAISTSLTG SAALKEKPPEAAVI
Sbjct: 481 TVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600
           VDSGVFHGGPPMSVQVQ+LVKFV+LSEMRKGPQDSPG+WMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQVKFG 600

Query: 601 LLHVVSETITDDQ 614
           LLHV+SETITDDQ
Sbjct: 601 LLHVLSETITDDQ 613

BLAST of CSPI05G19200 vs. ExPASy TrEMBL
Match: E5GB62 (MACPF domain-containing protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1226.8 bits (3173), Expect = 0.0e+00
Identity = 594/613 (96.90%), Postives = 604/613 (98.53%), Query Frame = 0

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIV+ ALNSLGKGFDLTSDFRLKYCKG  SLVLLNQSQRRDLIVPGFGAVKDVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP+SKPKDHHKPKIPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE IP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADNKPVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCT+PIKYDPAWATTSGD NSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHL LLYS+V NSYIVQTNWARHSSDISPKSGIFSAISTSLTG SAALKEKPPEAAVI
Sbjct: 481 TVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600
           VDSGVFHGGPPMSVQVQ+LVKFV+LSEMRKGPQDSPG+WMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQVKFG 600

Query: 601 LLHVVSETITDDQ 614
           LLHV+SETITDDQ
Sbjct: 601 LLHVLSETITDDQ 613

BLAST of CSPI05G19200 vs. ExPASy TrEMBL
Match: A0A1S3CSS8 (LOW QUALITY PROTEIN: MACPF domain-containing protein At1g14780-like OS=Cucumis melo OX=3656 GN=LOC103504476 PE=4 SV=1)

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 593/613 (96.74%), Postives = 603/613 (98.37%), Query Frame = 0

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIV+ ALNSLGKGFDLTSDFRLKYCKG  SLVLLNQSQRRDLIVPGFGAVKDVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP+SKPKDHHKPKIPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE IP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADNKPVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCT+PIKYDPAWATTSGD NSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
            VLHL LLYS+V NSYIVQTNWARHSSDISPKSGIFSAISTSLTG SAALKEKPPEAAVI
Sbjct: 481 XVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600
           VDSGVFHGGPPMSVQVQ+LVKFV+LSEMRKGPQDSPG+WMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQVKFG 600

Query: 601 LLHVVSETITDDQ 614
           LLHV+SETITDDQ
Sbjct: 601 LLHVLSETITDDQ 613

BLAST of CSPI05G19200 vs. ExPASy TrEMBL
Match: A0A5A7TTL6 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold149G001490 PE=4 SV=1)

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 567/613 (92.50%), Postives = 577/613 (94.13%), Query Frame = 0

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIV+ ALNSLGKGFDLTSDFRLKYCKG  SLVLLNQSQRRDLIVPGFGAVKDVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP+SKPKDHHKPK            
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPK------------ 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
                        GIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE IP
Sbjct: 241 -------------GIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQV  DNKPVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVNPDNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCT+PIKYDPAWATTSGD NSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHL LLYS+V NSYIVQTNWARHSSDISPKSGIFSAISTSLTG SAALKEKPPEAAVI
Sbjct: 481 TVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600
           VDSGVFHGGPPMSVQVQ+LVKFV+LSEMRKGPQDSPG+WMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQVKFG 588

Query: 601 LLHVVSETITDDQ 614
           LLHV+SETITDDQ
Sbjct: 601 LLHVLSETITDDQ 588

BLAST of CSPI05G19200 vs. NCBI nr
Match: XP_004142827.1 (MACPF domain-containing protein At1g14780 isoform X1 [Cucumis sativus] >KGN51464.1 hypothetical protein Csa_008302 [Cucumis sativus])

HSP 1 Score: 1261.5 bits (3263), Expect = 0.0e+00
Identity = 612/613 (99.84%), Postives = 613/613 (100.00%), Query Frame = 0

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI
Sbjct: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADN+PVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNRPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI
Sbjct: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600
           VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600

Query: 601 LLHVVSETITDDQ 614
           LLHVVSETITDDQ
Sbjct: 601 LLHVVSETITDDQ 613

BLAST of CSPI05G19200 vs. NCBI nr
Match: ADN33704.1 (hypothetical protein [Cucumis melo subsp. melo] >TYK14657.1 MACPF domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1226.8 bits (3173), Expect = 0.0e+00
Identity = 594/613 (96.90%), Postives = 604/613 (98.53%), Query Frame = 0

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIV+ ALNSLGKGFDLTSDFRLKYCKG  SLVLLNQSQRRDLIVPGFGAVKDVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP+SKPKDHHKPKIPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE IP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADNKPVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCT+PIKYDPAWATTSGD NSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHL LLYS+V NSYIVQTNWARHSSDISPKSGIFSAISTSLTG SAALKEKPPEAAVI
Sbjct: 481 TVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600
           VDSGVFHGGPPMSVQVQ+LVKFV+LSEMRKGPQDSPG+WMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQVKFG 600

Query: 601 LLHVVSETITDDQ 614
           LLHV+SETITDDQ
Sbjct: 601 LLHVLSETITDDQ 613

BLAST of CSPI05G19200 vs. NCBI nr
Match: XP_008467045.2 (PREDICTED: LOW QUALITY PROTEIN: MACPF domain-containing protein At1g14780-like [Cucumis melo])

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 593/613 (96.74%), Postives = 603/613 (98.37%), Query Frame = 0

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIV+ ALNSLGKGFDLTSDFRLKYCKG  SLVLLNQSQRRDLIVPGFGAVKDVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP+SKPKDHHKPKIPQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE IP
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVIADNKPVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCT+PIKYDPAWATTSGD NSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
            VLHL LLYS+V NSYIVQTNWARHSSDISPKSGIFSAISTSLTG SAALKEKPPEAAVI
Sbjct: 481 XVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600
           VDSGVFHGGPPMSVQVQ+LVKFV+LSEMRKGPQDSPG+WMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQVKFG 600

Query: 601 LLHVVSETITDDQ 614
           LLHV+SETITDDQ
Sbjct: 601 LLHVLSETITDDQ 613

BLAST of CSPI05G19200 vs. NCBI nr
Match: XP_038874758.1 (MACPF domain-containing protein At1g14780 [Benincasa hispida])

HSP 1 Score: 1180.2 bits (3052), Expect = 0.0e+00
Identity = 571/615 (92.85%), Postives = 590/615 (95.93%), Query Frame = 0

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           M DEIV+ ALNSLGKGFDLTSDFRLKYCKG+ SLVLLNQSQRRDLIVPGFGAV DVSIDI
Sbjct: 1   MGDEIVKIALNSLGKGFDLTSDFRLKYCKGEHSLVLLNQSQRRDLIVPGFGAVNDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWA DA+NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWAMDAMNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPS+WDP AIARF+EKYGTHIIVGLS+GG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPLAIARFVEKYGTHIIVGLSVGGHDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240
           VLVRQDKSS LEPSELKNHLEELGDQLFNGACTFPPP+SKPKDHHKPK PQAFNLFDPQL
Sbjct: 181 VLVRQDKSSNLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKTPQAFNLFDPQL 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
           VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE +P
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGMP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNI+T PSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTFPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQVI +NKPVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVIVENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSG--DVNSPAAFIVTGAQLLVVKQHD 480
           QHIAD+RYVE LNRNKFSHVCT PIKYDPAWATTSG  D  SP AFIVTGAQL VVKQHD
Sbjct: 421 QHIADERYVESLNRNKFSHVCTAPIKYDPAWATTSGNHDFPSPTAFIVTGAQLHVVKQHD 480

Query: 481 SKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAA 540
           SKTVLHL LLYS+V NSY+VQT+WARHSSDISPKSGIFSAISTSLTG +AALKEKPP+AA
Sbjct: 481 SKTVLHLRLLYSKVSNSYVVQTSWARHSSDISPKSGIFSAISTSLTGVAAALKEKPPDAA 540

Query: 541 VIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVK 600
           VIVDSGVFHGGPPMSVQ Q+LVKFV+LSEMRKGPQDSPGHWMVIGARLNLEKGKICL VK
Sbjct: 541 VIVDSGVFHGGPPMSVQAQKLVKFVELSEMRKGPQDSPGHWMVIGARLNLEKGKICLHVK 600

Query: 601 FGLLHVVSETITDDQ 614
           FGLLHVVSETITDDQ
Sbjct: 601 FGLLHVVSETITDDQ 615

BLAST of CSPI05G19200 vs. NCBI nr
Match: KAA0046404.1 (MACPF domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 567/613 (92.50%), Postives = 577/613 (94.13%), Query Frame = 0

Query: 1   MADEIVEKALNSLGKGFDLTSDFRLKYCKGQRSLVLLNQSQRRDLIVPGFGAVKDVSIDI 60
           MADEIV+ ALNSLGKGFDLTSDFRLKYCKG  SLVLLNQSQRRDLIVPGFGAVKDVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQL 240
           VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPP+SKPKDHHKPK            
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPK------------ 240

Query: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIP 300
                        GIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLE IP
Sbjct: 241 -------------GIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420
           GPKLYVNTTQV  DNKPVTGMRLYLEG+KSNRLAIHIQHLLTTPLLLENNITQQTYWHSS
Sbjct: 361 GPKLYVNTTQVNPDNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQTYWHSS 420

Query: 421 QHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVVKQHDSK 480
           QHIADDRYVEPLNRNKFSHVCT+PIKYDPAWATTSGD NSPAAFIVTGAQLLVVKQHDSK
Sbjct: 421 QHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATTSGDDNSPAAFIVTGAQLLVVKQHDSK 480

Query: 481 TVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKPPEAAVI 540
           TVLHL LLYS+V NSYIVQTNWARHSSDISPKSGIFSAISTSLTG SAALKEKPPEAAVI
Sbjct: 481 TVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAALKEKPPEAAVI 540

Query: 541 VDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKICLQVKFG 600
           VDSGVFHGGPPMSVQVQ+LVKFV+LSEMRKGPQDSPG+WMVIGARLNLEKGKICLQVKFG
Sbjct: 541 VDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGKICLQVKFG 588

Query: 601 LLHVVSETITDDQ 614
           LLHV+SETITDDQ
Sbjct: 601 LLHVLSETITDDQ 588

BLAST of CSPI05G19200 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 699.9 bits (1805), Expect = 1.8e-201
Identity = 350/616 (56.82%), Postives = 447/616 (72.56%), Query Frame = 0

Query: 4   EIVEKALNSLGKGFDLTSDFRLKYCK------GQRSLVLLNQSQRRDLIVPGFGAVKDVS 63
           +++E A+ SLGKGFDLT+DFRLKYCK      G   LV+L+Q+Q R+L +PGFG  ++VS
Sbjct: 7   DVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVS 66

Query: 64  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 123
            DI CD+G+RTR++SDIL+FN+MSE FN++ S+ GKIPSG+FN+ FGFQSG WATDA N 
Sbjct: 67  ADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANV 126

Query: 124 KCLGLDGYFISLFNAHI-DRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIG 183
           K LGLD   ++LFN HI +   LRL+D VR+AVPS+WDP  +ARFIE+YGTH+I G+S+G
Sbjct: 127 KSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVG 186

Query: 184 GRDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHH---KPKIPQAF 243
           G+DVV+VRQDKSS L+   L++HL +LGDQLF G+C           HH   +PK P+AF
Sbjct: 187 GQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF 246

Query: 244 NLF-DPQLVAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPI 303
           N+F D Q VAF NF S+++++GI VI +KRGG   A SH EWL TV + PDAI+F FIPI
Sbjct: 247 NVFDDKQTVAFNNF-SINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPI 306

Query: 304 ISLLECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTS 363
            SLL+ +PG G LSHA++LYLRYKPP+ DLQYFLDF   + WAP+HNDLP G  PN++++
Sbjct: 307 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASA 366

Query: 364 -PSLQLNLMGPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNI 423
            P+L +N MGPKLYVNTT V ++  PVTGMR +LEG K NRLAIH+QHL  T   +   I
Sbjct: 367 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 426

Query: 424 TQQTYWHSSQHIAD-DRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNS--PAAFIVTG 483
           T +  W  S  I D DRY EPLN  KFSHVCT+P+KYDP W  T+ +  S    AFIVTG
Sbjct: 427 TDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 486

Query: 484 AQLLVVKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSA 543
           AQ L VK+H SK+VLHL L Y++V + Y+VQ +W       S KSGIFS++S  LT  S 
Sbjct: 487 AQ-LEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSV 546

Query: 544 ALKE-KPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLN 603
                +  +  V++DSGVF GGPP+     ++VKFVDLS++ +GPQ SPGHW+V G RL 
Sbjct: 547 HHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLY 606

BLAST of CSPI05G19200 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 501.9 bits (1291), Expect = 7.3e-142
Identity = 272/607 (44.81%), Postives = 379/607 (62.44%), Query Frame = 0

Query: 2   ADEIVEKALNSLGKGFDLTSDFRLKYCKG----QRSLVLLNQSQRRDLIVPGFGAVKDVS 61
           A +  E A+ S+G G+DL  D RLKYCKG     R L +       ++++PG  ++ +VS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 62  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 121
             IKCD+G+R R++SDIL F QM+E FN++ S+ GKIPSG FN+MF F S CW  DA  T
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYT 126

Query: 122 KCLGLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG 181
           K L  DG FISL++  +D+  + L + V+ AVPSTWDP A+ARFI+ YGTHIIV + +GG
Sbjct: 127 KNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGG 186

Query: 182 RDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFD 241
           +DV+  +Q  SSKL+P +L+  L+E+ D+ F  A       S+          +   L  
Sbjct: 187 KDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRF 246

Query: 242 PQLVAFGNFSSVSAKDGIIVITSKRGGSPTAS-SHCEWLPTVHESPDAIHFQFIPIISLL 301
               + G++++   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL
Sbjct: 247 ADTSSLGSYAN---KEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLL 306

Query: 302 ECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQ 361
             +PG GFLSHAINLYLRYKPPI +L  FL+FQ+ + WAP+ ++LPLGP     +  SLQ
Sbjct: 307 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQ 366

Query: 362 LNLMGPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLL--LENNITQQ 421
            +  GPKLYVNTT V    +P+TGMRLYLEG +SNRLAIH+QHL + P +  LE+++ + 
Sbjct: 367 FSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRS 426

Query: 422 TYWHSSQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLLVV 481
                 Q   D RY E +N   +SHVCT P++ D        D++     +VTGAQ L V
Sbjct: 427 I----RQESHDRRYYEKVNWKNYSHVCTEPVESD-------DDLS-----VVTGAQ-LHV 486

Query: 482 KQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKEKP 541
           + H  K VL L L +SRV  + +V+ +    +   +PKSG+ S + +     +AA K  P
Sbjct: 487 ESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISH--HFTAAQKPPP 546

Query: 542 PEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGKIC 601
             A V ++S ++ GGPP+  Q  +L+KFVD SEM +GPQ+SPG+W+V GARL +EKGKI 
Sbjct: 547 RPADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKIS 590

BLAST of CSPI05G19200 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 479.2 bits (1232), Expect = 5.0e-135
Identity = 271/616 (43.99%), Postives = 377/616 (61.20%), Query Frame = 0

Query: 2   ADEIVEKALNSLGKGFDLTSDFRLKYCK----GQRSLVLLNQSQRRDLIVPGFGAVKDVS 61
           A    EKA++ +G G+DL SD R   CK    G R LV ++ ++ RDLI PG   V +VS
Sbjct: 11  AHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSR-LVEIDPTRNRDLIFPGGIVVNNVS 70

Query: 62  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 121
             IKCD+G+RTR +SDIL+FNQMSE FN+   + GKIPSG FN+MF F S CW  DA + 
Sbjct: 71  SSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAF-SKCWPKDASSV 130

Query: 122 KCLGLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG 181
           K L  DG+FISL++  I R  L L DEV+  VPS+WD  A+A FIEKYGTH++VG+++GG
Sbjct: 131 KTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGG 190

Query: 182 RDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFD 241
           +DV+ V+Q + S  EP E++  L+  GD+ F   C  P     P   +  K P+  NL  
Sbjct: 191 KDVIHVKQMRKSNHEPEEIQKMLKHWGDERF---CVDPVESKSPASVYSGK-PKEENLLQ 250

Query: 242 PQLVAFGNFSSVSA-----KDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPI 301
             L  FG   S +       + I+ +  +RGG     SH  WL TV ++P+ I   F+PI
Sbjct: 251 WGLQPFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPI 310

Query: 302 ISLLECIPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTS 361
            SLL  +PG GFLSHA+NLYLRYKPPI +L  FL+FQ+ + WAP++ DLPLG   +  +S
Sbjct: 311 TSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSS 370

Query: 362 PSLQLNLMGPKLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNIT 421
           PSLQ +LMGPKLYVNT++V +  +PVTG+R +LEG K N LAIH+QHL   P  L  +++
Sbjct: 371 PSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSL--HLS 430

Query: 422 QQTYWHSSQHIADDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLL 481
               +   +   +  Y  P+    FSHVCT P++Y       +G  +   A IVT A  L
Sbjct: 431 HDDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQY-------NGARSDDTASIVTKA-WL 490

Query: 482 VVKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSL-TGTSAALK 541
            VK    + VL L L +S   ++   ++ W   S++ S KSG+FS IST L TG S    
Sbjct: 491 EVKGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTN-SRKSGVFSMISTRLSTGLSPNPA 550

Query: 542 EKPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKG 601
              P++ + ++S V+  GP   V+  +L+  VD  E+ +GP++ PG+W+V GA+L +E G
Sbjct: 551 TTKPQSKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAG 608

Query: 602 KICLQVKFGLLHVVSE 608
           KI ++ K+ LL V+SE
Sbjct: 611 KISIKAKYSLLTVISE 608

BLAST of CSPI05G19200 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 414.1 bits (1063), Expect = 2.0e-115
Identity = 247/611 (40.43%), Postives = 339/611 (55.48%), Query Frame = 0

Query: 6   VEKALNSLGKGFDLTSDFRLKYCKGQ--RSLVLLNQSQRRDLIVPGFGAVKDVSIDIKCD 65
           +  A+ +LG+GFD+TSD RL YCKG     LV + + Q RDL +     + +V  DI C 
Sbjct: 21  LRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCS 80

Query: 66  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCLGLD 125
           RG+    +  + +F++M+E FN +  + G IP G FN+MF + +G W  DA +TK L L 
Sbjct: 81  RGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNY-TGSWQVDAASTKSLALV 140

Query: 126 GYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGRDVVLV 185
           GYFI L++  + +  L L +E+R AVPS+WDP ++A FIE YGTHI+  ++IGGRDVV +
Sbjct: 141 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 200

Query: 186 RQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFDPQLVAF 245
           RQ +SS L  SE++N++ ++                         I   F+  + Q +  
Sbjct: 201 RQHQSSPLPVSEIENYVNDM-------------------------IKHRFHEAESQSIT- 260

Query: 246 GNFSSVSAKD-GIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLECIPGK 305
                +  KD  I VI  +RGG     SH  W  TV  +PD I+  F PI+SLLE +PG 
Sbjct: 261 ---GPLKYKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGL 320

Query: 306 GFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLMGP 365
             L+ AI LYL YKPPI DLQYFLD+Q+ + WAP  ++L            SLQ +LMGP
Sbjct: 321 RHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGP 380

Query: 366 KLYVNTTQVIADNKPVTGMRLYLEGMKSNRLAIHIQHLLTTPLLLENNITQQTYWHSSQH 425
           KL+++  QV    KPVTG+RL LEG K NRL+IH+QHL++ P +L      Q +W S   
Sbjct: 381 KLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKIL------QPHWDSHVP 440

Query: 426 IA----------DDRYVEPLNRNKFSHVCTIPIKYDPAWATTSGDVNSPAAFIVTGAQLL 485
           I           D R+ EP+    FSHV T PI++     T  GD++     IVTGAQ L
Sbjct: 441 IGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHT---ETHIGDLS--GVHIVTGAQ-L 500

Query: 486 VVKQHDSKTVLHLGLLYSRVPNSYIVQTNWARHSSDISPKSGIFSAISTSLTGTSAALKE 545
            V    SK VLHL LL+S+VP   I ++ W     D +P              +S  L+ 
Sbjct: 501 GVWDFGSKNVLHLKLLFSKVPGCTIRRSVW-----DHTP------------VASSGRLEP 560

Query: 546 KPPEAAVIVDSGVFHGGPPMSVQVQRLVKFVDLSEMRKGPQDSPGHWMVIGARLNLEKGK 604
             P  +   +         +S Q  +L K VD SEM KGPQD PGHW+V GA+L +EKGK
Sbjct: 561 GGPSTSSSTEE--------VSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGK 560

BLAST of CSPI05G19200 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 273.9 bits (699), Expect = 3.2e-73
Identity = 143/321 (44.55%), Postives = 198/321 (61.68%), Query Frame = 0

Query: 2   ADEIVEKALNSLGKGFDLTSDFRLKYCKG----QRSLVLLNQSQRRDLIVPGFGAVKDVS 61
           A +  E A+ S+G G+DL  D RLKYCKG     R L +       ++++PG  ++ +VS
Sbjct: 7   ASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVS 66

Query: 62  IDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNT 121
             IKCD+G+R R++SDIL F QM+E FN++ S+ GKIPSG FN+MF F S CW  DA  T
Sbjct: 67  KSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYT 126

Query: 122 KCLGLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGG 181
           K L  DG FISL++  +D+  + L + V+ AVPSTWDP A+ARFI+ YGTHIIV + +GG
Sbjct: 127 KNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGG 186

Query: 182 RDVVLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPVSKPKDHHKPKIPQAFNLFD 241
           +DV+  +Q  SSKL+P +L+  L+E+ D+ F  A       S+          +   L  
Sbjct: 187 KDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRF 246

Query: 242 PQLVAFGNFSSVSAKDGIIVITSKRGGSPTAS-SHCEWLPTVHESPDAIHFQFIPIISLL 301
               + G++++   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL
Sbjct: 247 ADTSSLGSYAN---KEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLL 306

Query: 302 ECIPGKGFLSHAINLYLRYKP 318
             +PG GFLSHAINLYLR KP
Sbjct: 307 NGVPGSGFLSHAINLYLRCKP 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L6122.6e-20056.82MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Q9STW51.0e-14044.81MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4... [more]
Q9SGN67.1e-13443.99MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=... [more]
Q9C7N22.8e-11440.43MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0KRG00.0e+0099.84MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G551250 PE=4 ... [more]
A0A5D3CRV50.0e+0096.90MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
E5GB620.0e+0096.90MACPF domain-containing protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3CSS80.0e+0096.74LOW QUALITY PROTEIN: MACPF domain-containing protein At1g14780-like OS=Cucumis m... [more]
A0A5A7TTL60.0e+0092.50MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
Match NameE-valueIdentityDescription
XP_004142827.10.0e+0099.84MACPF domain-containing protein At1g14780 isoform X1 [Cucumis sativus] >KGN51464... [more]
ADN33704.10.0e+0096.90hypothetical protein [Cucumis melo subsp. melo] >TYK14657.1 MACPF domain-contain... [more]
XP_008467045.20.0e+0096.74PREDICTED: LOW QUALITY PROTEIN: MACPF domain-containing protein At1g14780-like [... [more]
XP_038874758.10.0e+0092.85MACPF domain-containing protein At1g14780 [Benincasa hispida][more]
KAA0046404.10.0e+0092.50MACPF domain-containing protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G14780.11.8e-20156.82MAC/Perforin domain-containing protein [more]
AT4G24290.27.3e-14244.81MAC/Perforin domain-containing protein [more]
AT1G28380.15.0e-13543.99MAC/Perforin domain-containing protein [more]
AT1G29690.12.0e-11540.43MAC/Perforin domain-containing protein [more]
AT4G24290.13.2e-7344.55MAC/Perforin domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020864Membrane attack complex component/perforin (MACPF) domainSMARTSM00457MACPF_8coord: 117..314
e-value: 0.0013
score: 24.7
IPR020864Membrane attack complex component/perforin (MACPF) domainPFAMPF01823MACPFcoord: 98..312
e-value: 1.3E-25
score: 90.8
IPR020864Membrane attack complex component/perforin (MACPF) domainPROSITEPS51412MACPF_2coord: 1..326
score: 21.897575
NoneNo IPR availablePANTHERPTHR33199:SF4OS02G0736300 PROTEINcoord: 4..604
IPR044663MACPF domain-containing protein CAD1/NSL1-likePANTHERPTHR33199MACPF DOMAIN-CONTAINING PROTEIN CAD1coord: 4..604

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G19200.1CSPI05G19200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009626 plant-type hypersensitive response
biological_process GO:2000031 regulation of salicylic acid mediated signaling pathway
biological_process GO:0006952 defense response
biological_process GO:0012501 programmed cell death