CSPI05G14260 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G14260
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionProlamin_like domain-containing protein
LocationChr5: 14574660 .. 14575061 (-)
RNA-Seq ExpressionCSPI05G14260
SyntenyCSPI05G14260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAACTCGACTCGAGTGCTATTAGCGGTGGTGATGCTATCATTACTTGTAGTAGACTCGTTGGCCTTAGTTCAACCTTCTACGGTAGATGACAATGGCATATCATCAATATGGAGTGATTGGTTAGATGAAGATAGAGCACCTACTCTAGCACCGTGTTTACACATGATGAGGAGTGAAAAATGCCAAGTGGAACTTTATAATTATTACTTCAATATAAGCAAGAAGGAATTGGATCTAAGTTGTTGCGTATATGTCAATTACATGGGCAAGAAATGTGCTGCAGCATTTGAGTTTTGGTTCTCGTTCCCTAGTTTAGAAGCCTTAAAGCCTAATCCAATGAAGGTCTACAACAATTGTTTTAAGAGATTGACTTTTCCAGCACCAACGCCACTTTAA

mRNA sequence

ATGAACAACTCGACTCGAGTGCTATTAGCGGTGGTGATGCTATCATTACTTGTAGTAGACTCGTTGGCCTTAGTTCAACCTTCTACGGTAGATGACAATGGCATATCATCAATATGGAGTGATTGGTTAGATGAAGATAGAGCACCTACTCTAGCACCGTGTTTACACATGATGAGGAGTGAAAAATGCCAAGTGGAACTTTATAATTATTACTTCAATATAAGCAAGAAGGAATTGGATCTAAGTTGTTGCGTATATGTCAATTACATGGGCAAGAAATGTGCTGCAGCATTTGAGTTTTGGTTCTCGTTCCCTAGTTTAGAAGCCTTAAAGCCTAATCCAATGAAGGTCTACAACAATTGTTTTAAGAGATTGACTTTTCCAGCACCAACGCCACTTTAA

Coding sequence (CDS)

ATGAACAACTCGACTCGAGTGCTATTAGCGGTGGTGATGCTATCATTACTTGTAGTAGACTCGTTGGCCTTAGTTCAACCTTCTACGGTAGATGACAATGGCATATCATCAATATGGAGTGATTGGTTAGATGAAGATAGAGCACCTACTCTAGCACCGTGTTTACACATGATGAGGAGTGAAAAATGCCAAGTGGAACTTTATAATTATTACTTCAATATAAGCAAGAAGGAATTGGATCTAAGTTGTTGCGTATATGTCAATTACATGGGCAAGAAATGTGCTGCAGCATTTGAGTTTTGGTTCTCGTTCCCTAGTTTAGAAGCCTTAAAGCCTAATCCAATGAAGGTCTACAACAATTGTTTTAAGAGATTGACTTTTCCAGCACCAACGCCACTTTAA

Protein sequence

MNNSTRVLLAVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRSEKCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNNCFKRLTFPAPTPL*
Homology
BLAST of CSPI05G14260 vs. ExPASy TrEMBL
Match: A0A0A0KQ41 (Prolamin_like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G381840 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.6e-70
Identity = 132/133 (99.25%), Postives = 132/133 (99.25%), Query Frame = 0

Query: 1   MNNSTRVLLAVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRS 60
           MNNSTRVLL VVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRS
Sbjct: 1   MNNSTRVLLVVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRS 60

Query: 61  EKCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNN 120
           EKCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNN
Sbjct: 61  EKCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNN 120

Query: 121 CFKRLTFPAPTPL 134
           CFKRLTFPAPTPL
Sbjct: 121 CFKRLTFPAPTPL 133

BLAST of CSPI05G14260 vs. ExPASy TrEMBL
Match: A0A0A0L535 (Prolamin_like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G008300 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.0e-51
Identity = 100/132 (75.76%), Postives = 115/132 (87.12%), Query Frame = 0

Query: 2   NNSTRVLLAVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRSE 61
           +NST  LL VVMLSLLVVDSLALV PS +DDNGISSIWS W+D+DRAP LA CL++M+S+
Sbjct: 5   SNSTGALLLVVMLSLLVVDSLALVHPSIIDDNGISSIWSGWIDKDRAPILASCLYLMKSD 64

Query: 62  KCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNNC 121
           KCQVELYNYYFN SKKE+DLSCCVYVNY+G++CAA+FE WFS    +ALKPNPMK+YNNC
Sbjct: 65  KCQVELYNYYFNKSKKEVDLSCCVYVNYIGEECAASFESWFSSLGFKALKPNPMKLYNNC 124

Query: 122 FKRLTFPAPTPL 134
           FKRLT PAP  L
Sbjct: 125 FKRLTVPAPPTL 136

BLAST of CSPI05G14260 vs. ExPASy TrEMBL
Match: A0A0A0KEB8 (Prolamin_like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G054340 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.6e-41
Identity = 87/133 (65.41%), Postives = 101/133 (75.94%), Query Frame = 0

Query: 1   MNNSTRVLLAVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRS 60
           M N T  + A+V+L L  ++   LVQ S VD++  SSIW+DWLDEDRA TL PCL  MRS
Sbjct: 1   MANKTNAMRAMVIL-LSAINLAKLVQSSGVDEDYNSSIWNDWLDEDRAFTLRPCLDRMRS 60

Query: 61  EKCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNN 120
            KCQVELYNYYFNISKKELDL+CCV+V  MGKKCA AF FW+ FP LE  +PNPMKVYNN
Sbjct: 61  AKCQVELYNYYFNISKKELDLNCCVFVKVMGKKCAQAFTFWYQFPGLEIYEPNPMKVYNN 120

Query: 121 CFKRLTFPAPTPL 134
           C  RL+ P PTP+
Sbjct: 121 CATRLSAPPPTPI 132

BLAST of CSPI05G14260 vs. ExPASy TrEMBL
Match: A0A5D3C280 (Prolamin_like domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G001130 PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.8e-37
Identity = 76/124 (61.29%), Postives = 89/124 (71.77%), Query Frame = 0

Query: 9   LAVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRSEKCQVELY 68
           +  +++ L  ++   LVQ S VD++  S IW  W+DEDR  TL PCL  MRS KCQVELY
Sbjct: 1   MRAIVILLFAINLSKLVQSSGVDEDYNSPIWDGWVDEDRGYTLRPCLDRMRSAKCQVELY 60

Query: 69  NYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNNCFKRLTFP 128
           NYYFNISKKELDL+CCV+V  MGKKCA AF FW+ FP LE  +PNPMKVYNNC  RL  P
Sbjct: 61  NYYFNISKKELDLNCCVFVKVMGKKCAQAFTFWYQFPGLEIYEPNPMKVYNNCITRLDAP 120

Query: 129 APTP 133
            P P
Sbjct: 121 PPAP 124

BLAST of CSPI05G14260 vs. ExPASy TrEMBL
Match: A0A5A7TZR1 (Prolamin_like domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G001070 PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 5.0e-35
Identity = 78/134 (58.21%), Postives = 91/134 (67.91%), Query Frame = 0

Query: 1   MNNSTRVLLAVVMLSLLVVDSLALVQPS--TVDDNGISSIWSDWLDEDRAPTLAPCLHMM 60
           M N T  +  ++ + LL V+   LV PS   VDD+  S IW+ W+DEDRA  L PCL MM
Sbjct: 1   MANGTNTVGGILTILLLAVNLAILVHPSMADVDDDYNSPIWNGWVDEDRASNLRPCLDMM 60

Query: 61  RSEKCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVY 120
           +S KCQV+LYNYYFNISKKELDL CCV+V  MGK CA AF  W+ FP LE  +PNPM VY
Sbjct: 61  KSAKCQVQLYNYYFNISKKELDLQCCVFVKIMGKYCAHAFAGWYEFPGLEVYEPNPMIVY 120

Query: 121 NNCFKRLTFPAPTP 133
           NNC  RL    PTP
Sbjct: 121 NNCVGRLQALPPTP 134

BLAST of CSPI05G14260 vs. NCBI nr
Match: KGN50979.1 (hypothetical protein Csa_008880 [Cucumis sativus])

HSP 1 Score: 275.0 bits (702), Expect = 3.4e-70
Identity = 132/133 (99.25%), Postives = 132/133 (99.25%), Query Frame = 0

Query: 1   MNNSTRVLLAVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRS 60
           MNNSTRVLL VVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRS
Sbjct: 1   MNNSTRVLLVVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRS 60

Query: 61  EKCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNN 120
           EKCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNN
Sbjct: 61  EKCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNN 120

Query: 121 CFKRLTFPAPTPL 134
           CFKRLTFPAPTPL
Sbjct: 121 CFKRLTFPAPTPL 133

BLAST of CSPI05G14260 vs. NCBI nr
Match: KAE8650019.1 (hypothetical protein Csa_009940 [Cucumis sativus])

HSP 1 Score: 192.2 bits (487), Expect = 2.9e-45
Identity = 90/119 (75.63%), Postives = 105/119 (88.24%), Query Frame = 0

Query: 2   NNSTRVLLAVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRSE 61
           +NST  LL VVMLSLLVVDSLALV PS +DDNGISSIWS W+D+DRAP LA CL++M+S+
Sbjct: 5   SNSTGALLLVVMLSLLVVDSLALVHPSIIDDNGISSIWSGWIDKDRAPILASCLYLMKSD 64

Query: 62  KCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNN 121
           KCQVELYNYYFN SKKE+DLSCCVYVNY+G++CAA+FE WFS    +ALKPNPMK+YNN
Sbjct: 65  KCQVELYNYYFNKSKKEVDLSCCVYVNYIGEECAASFESWFSSLGFKALKPNPMKLYNN 123

BLAST of CSPI05G14260 vs. NCBI nr
Match: KAA0046835.1 (hypothetical protein E6C27_scaffold216G001330 [Cucumis melo var. makuwa] >KAA0046840.1 hypothetical protein E6C27_scaffold216G001400 [Cucumis melo var. makuwa] >KAA0065932.1 hypothetical protein E6C27_scaffold10942G00010 [Cucumis melo var. makuwa] >TYK04499.1 hypothetical protein E5676_scaffold409G001130 [Cucumis melo var. makuwa] >TYK14838.1 hypothetical protein E5676_scaffold6575G00010 [Cucumis melo var. makuwa])

HSP 1 Score: 165.2 bits (417), Expect = 3.8e-37
Identity = 76/124 (61.29%), Postives = 89/124 (71.77%), Query Frame = 0

Query: 9   LAVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRSEKCQVELY 68
           +  +++ L  ++   LVQ S VD++  S IW  W+DEDR  TL PCL  MRS KCQVELY
Sbjct: 1   MRAIVILLFAINLSKLVQSSGVDEDYNSPIWDGWVDEDRGYTLRPCLDRMRSAKCQVELY 60

Query: 69  NYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNNCFKRLTFP 128
           NYYFNISKKELDL+CCV+V  MGKKCA AF FW+ FP LE  +PNPMKVYNNC  RL  P
Sbjct: 61  NYYFNISKKELDLNCCVFVKVMGKKCAQAFTFWYQFPGLEIYEPNPMKVYNNCITRLDAP 120

Query: 129 APTP 133
            P P
Sbjct: 121 PPAP 124

BLAST of CSPI05G14260 vs. NCBI nr
Match: KAA0046841.1 (hypothetical protein E6C27_scaffold216G001410 [Cucumis melo var. makuwa] >TYK04493.1 hypothetical protein E5676_scaffold409G001070 [Cucumis melo var. makuwa])

HSP 1 Score: 157.1 bits (396), Expect = 1.0e-34
Identity = 78/134 (58.21%), Postives = 91/134 (67.91%), Query Frame = 0

Query: 1   MNNSTRVLLAVVMLSLLVVDSLALVQPS--TVDDNGISSIWSDWLDEDRAPTLAPCLHMM 60
           M N T  +  ++ + LL V+   LV PS   VDD+  S IW+ W+DEDRA  L PCL MM
Sbjct: 1   MANGTNTVGGILTILLLAVNLAILVHPSMADVDDDYNSPIWNGWVDEDRASNLRPCLDMM 60

Query: 61  RSEKCQVELYNYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVY 120
           +S KCQV+LYNYYFNISKKELDL CCV+V  MGK CA AF  W+ FP LE  +PNPM VY
Sbjct: 61  KSAKCQVQLYNYYFNISKKELDLQCCVFVKIMGKYCAHAFAGWYEFPGLEVYEPNPMIVY 120

Query: 121 NNCFKRLTFPAPTP 133
           NNC  RL    PTP
Sbjct: 121 NNCVGRLQALPPTP 134

BLAST of CSPI05G14260 vs. NCBI nr
Match: KAG6578867.1 (hypothetical protein SDJN03_23315, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 154.1 bits (388), Expect = 8.7e-34
Identity = 72/124 (58.06%), Postives = 85/124 (68.55%), Query Frame = 0

Query: 9   LAVVMLSLLVVDSLALVQPSTVDDNGISSIWSDWLDEDRAPTLAPCLHMMRSEKCQVELY 68
           +  +++ LL ++   L QPST DD+  S IW  W+DEDR   L PCL MM+S KCQVELY
Sbjct: 1   MGAMVILLLAINLAFLAQPSTADDDYNSPIWDRWVDEDRGINLRPCLDMMKSAKCQVELY 60

Query: 69  NYYFNISKKELDLSCCVYVNYMGKKCAAAFEFWFSFPSLEALKPNPMKVYNNCFKRLTFP 128
           NYYFNISKKELDL+CCV+V  MGK+CA AF  WF FP  E  + NPMKVY NC  RL   
Sbjct: 61  NYYFNISKKELDLNCCVFVKVMGKRCARAFAGWFRFPEFEVFELNPMKVYKNCVGRLDAL 120

Query: 129 APTP 133
            P P
Sbjct: 121 PPAP 124

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KQ411.6e-7099.25Prolamin_like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G3818... [more]
A0A0A0L5351.0e-5175.76Prolamin_like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G0083... [more]
A0A0A0KEB81.6e-4165.41Prolamin_like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G0543... [more]
A0A5D3C2801.8e-3761.29Prolamin_like domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A5A7TZR15.0e-3558.21Prolamin_like domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
KGN50979.13.4e-7099.25hypothetical protein Csa_008880 [Cucumis sativus][more]
KAE8650019.12.9e-4575.63hypothetical protein Csa_009940 [Cucumis sativus][more]
KAA0046835.13.8e-3761.29hypothetical protein E6C27_scaffold216G001330 [Cucumis melo var. makuwa] >KAA004... [more]
KAA0046841.11.0e-3458.21hypothetical protein E6C27_scaffold216G001410 [Cucumis melo var. makuwa] >TYK044... [more]
KAG6578867.18.7e-3458.06hypothetical protein SDJN03_23315, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008502Prolamin-like domainPFAMPF05617Prolamin_likecoord: 54..121
e-value: 1.2E-6
score: 28.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G14260.1CSPI05G14260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0110165 cellular anatomical entity