Homology
BLAST of CSPI05G10020 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 880.2 bits (2273), Expect = 1.7e-254
Identity = 457/739 (61.84%), Postives = 556/739 (75.24%), Query Frame = 0
Query: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLRE 60
MSSSLIFKL + S+ LAS DS +D + IYIVYMG K +D S HH ML +
Sbjct: 1 MSSSLIFKLFFFSL--FFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQ 60
Query: 61 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 120
V GS FAPES+LH+YKRSFNGF VKLTEEEA +I++ EGVVSVF + LHTTRSWDF+
Sbjct: 61 VVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFL 120
Query: 121 GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKI 180
GF VPR +QVES+IVVGVLD+GIWPE+PSF D G+ P P KWKG C+ NF CN+KI
Sbjct: 121 GFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKI 180
Query: 181 IGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR 240
IGAR+Y D+ PRD+NGHGTHTAST AGGLVSQA+LYGL LGTARGGVP AR
Sbjct: 181 IGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLAR 240
Query: 241 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG 300
IA YK+CW+DGCSD DILAA+DDAIADGVDIISLSVGG+ R+YF D+IAIG+FH+++ G
Sbjct: 241 IAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERG 300
Query: 301 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG 360
ILTSNSAGN GP++FT + SPW LSVAAST DRK V++V+IGN +QG +INTFD
Sbjct: 301 ILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--N 360
Query: 361 KQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSD-AV 420
+ YPL+ D PN GF S SRFC++ SV+ NL+ GKI++C++ P F D A
Sbjct: 361 QYYPLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAA 420
Query: 421 GVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIV 480
GV+M + Y ++SYPLPSS L+ D A Y+ S P ATIFKS + +++AP +V
Sbjct: 421 GVLMTSNTRDY-ADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVV 480
Query: 481 SFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPH 540
SFSSRGPN T D++KPD++ PGVEILAAW +APV G+ R TL+NIISGTSMSCPH
Sbjct: 481 SFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPV-GGI--RRNTLFNIISGTSMSCPH 540
Query: 541 ATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVY 600
T A YVKT++PTWSPAAIKSALMTTA+P+ N +AEFAYG+G +NPLKA+ PGLVY
Sbjct: 541 ITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVY 600
Query: 601 DANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQF 660
DANE DYVKFLCGQGY + V+ ++ D + C S N GRVWDLNYPSF LS +PSQ+ NQ+
Sbjct: 601 DANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQY 660
Query: 661 FTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGIGEKKTFTLTIQGTIDPTTIV 720
F RTLTSV ASTY + ++ APQGLTI+VNP VL F+G+G++K+FTLT++G+I +V
Sbjct: 661 FNRTLTSVAPQASTYRA-MISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSI-KGFVV 720
Query: 721 SASLVWSDSSHDVRSPITI 739
SASLVWSD H VRSPITI
Sbjct: 721 SASLVWSDGVHYVRSPITI 727
BLAST of CSPI05G10020 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 644.8 bits (1662), Expect = 1.2e-183
Identity = 343/720 (47.64%), Postives = 466/720 (64.72%), Query Frame = 0
Query: 28 SKNDDRK---IYIVYMGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVV 87
S ND R+ +YIVYMG P+ S PSHH+ +L+++ G+ A L+ SYKRSFNGF
Sbjct: 22 SANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAA 81
Query: 88 KLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFIGFTKDVPRVNQVESDIVVGVLDSG 147
L++ E+ ++ + VVSVFPS L TTRSWDF+GF + R + ESD++VGV+DSG
Sbjct: 82 NLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSG 141
Query: 148 IWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKIIGARAYRSDNVFPTEDIPSPRDSN 207
IWPE+ SF D G+GP P KWKG C+ F CN K+IGAR Y N F S RD
Sbjct: 142 IWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY---NKF----ADSARDEE 201
Query: 208 GHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDA 267
GHGTHTAST AG V AS YGLA GTARGGVPSARIA YK+C+ + C+D DILAAFDDA
Sbjct: 202 GHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDA 261
Query: 268 IADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWS 327
IADGVD+IS+S+ N S+AIG+FH+M GI+T+ SAGN+GPD ++ N SPW
Sbjct: 262 IADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWM 321
Query: 328 LSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPNLIGGFTGSISR 387
++VAAS TDR+ + RV +GN G ++NTF+ G ++P++Y N+ + + +
Sbjct: 322 ITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAG 381
Query: 388 FCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVGVVMNDDGVKYPSNSYPLPSSYLET 447
+CS G VD+ LV GKI+LCD L Y + A+GV++ + + + P P+S L
Sbjct: 382 YCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGF 441
Query: 448 VDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIVSFSSRGPNPETLDILKPDLTAPGVE 507
D +IK+Y+ S P A I +++ + D AP++ SFSSRGP+ ++LKPD++APG+E
Sbjct: 442 EDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLE 501
Query: 508 ILAAWSPIAPVSS--GVIDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSA 567
ILAA+SP+A SS D R+ Y+++SGTSM+CPH A YVK+FHP WSP+AIKSA
Sbjct: 502 ILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSA 561
Query: 568 LMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYDANEFDYVKFLCGQGYTSDMVQS 627
+MTTATP+ + N E EFAYG+GQINP KA PGLVY+ DY+K LC +G+ S + +
Sbjct: 562 IMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTT 621
Query: 628 LSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAP 687
S N C+ V DLNYP+ + N F RT+T+V STY ++++
Sbjct: 622 TSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQ 681
Query: 688 QGLTITVNPKVLLFSGIGEKKTFTLTIQG-TIDPTTIVSASLVWSDSSHDVRSPITIYVV 742
L I++ P++L F + EKK+F +TI G + + VS+S+VWSD SH VRSPI Y +
Sbjct: 682 PELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727
BLAST of CSPI05G10020 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 610.9 bits (1574), Expect = 1.9e-173
Identity = 331/750 (44.13%), Postives = 475/750 (63.33%), Query Frame = 0
Query: 3 SSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTP-SHHMRMLREV 62
++ IF + +LVL L S S + D+++YIVY+G+ P TP S HM +L+E+
Sbjct: 5 TTFIFLFSSLLVLSLSS---VSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEI 64
Query: 63 TGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFIG 122
TG + L+ SYK+SFNGF +LTE E R++ E VVSVFPS K L TT SW+F+G
Sbjct: 65 TGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMG 124
Query: 123 FTKDV--PRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKK 182
+ + R +ESD ++GV+DSGI+PE+ SFSD G+GP P KWKG C NFTCN K
Sbjct: 125 LKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNK 184
Query: 183 IIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSA 242
+IGAR Y + ++ + RD +GHGTHTAS AG V+ ++ YGL GTARGGVP+A
Sbjct: 185 VIGARDYTA----KSKANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAA 244
Query: 243 RIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKH 302
RIAVYK+C ++GC +++AFDDAIADGVD+IS+S+ + D IAIGAFH+M
Sbjct: 245 RIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAV 304
Query: 303 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPL 362
G+LT N+AGN+GP T+ + +PW SVAAS T+R +++V +G+ + G ++NT+D
Sbjct: 305 GVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMN 364
Query: 363 GKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAV 422
G YPL+Y A + + +R C +D LV GKI+LCDS
Sbjct: 365 GTNYPLVYGKSA--ALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVG 424
Query: 423 GVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIV 482
+V N + + S+P+ S+L D ++ +YM S P AT+ KS+ +++ AP +
Sbjct: 425 SIVKNPEPDRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVA 484
Query: 483 SFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPH 542
SFSSRGP+ DILKPD+TAPGVEILAA+SP + + D+R Y+++SGTSM+CPH
Sbjct: 485 SFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPH 544
Query: 543 ATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEIN--VEAEFAYGAGQINPLKAISPGL 602
A YVKTFHP WSP+ I+SA+MTTA P+ + V EFAYG+G ++P+ AI+PGL
Sbjct: 545 VAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGL 604
Query: 603 VYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICN---SANIGRVWDLNYPSFALSSTPSQ 662
VY+ + D++ FLCG YTSD ++ +S DN+ C S + R +LNYP+ + + ++
Sbjct: 605 VYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGTK 664
Query: 663 SINQFFTRTLTSVDSNASTYTSTILGAP-QGLTITVNPKVLLFSGIGEKKTFTLTIQG-T 722
N F RT+T+V STY + ++ P L+I V+P+VL + EK++F +T+ +
Sbjct: 665 PFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDS 724
Query: 723 IDPTTIVSASLVWSDSSHDVRSPITIYVVT 743
I VSA+L+WSD +H+VRSPI +Y ++
Sbjct: 725 IGTKQPVSANLIWSDGTHNVRSPIIVYAMS 741
BLAST of CSPI05G10020 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 608.6 bits (1568), Expect = 9.4e-173
Identity = 339/752 (45.08%), Postives = 466/752 (61.97%), Query Frame = 0
Query: 2 SSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTP-SHHMRMLRE 61
+SS + LVL L VS++ DD+++YIVYMG+ TP S HM +L+E
Sbjct: 6 ASSSLLSCLLVLFLSSVSAV--------TDDKQVYIVYMGSLSSRADYTPTSDHMNILQE 65
Query: 62 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 121
VTG + L+ SYKRSFNGF +LTE E R++ GVVSVFP+ K L TT SWDF+
Sbjct: 66 VTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFM 125
Query: 122 GFTKDV--PRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNK 181
G + + R VESD ++GV+DSGI PE+ SFSD G+GP P KWKG+C NFTCN
Sbjct: 126 GLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNN 185
Query: 182 KIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPS 241
K+IGAR Y S+ RD +GHGTHTAST AG V AS +G+ GT RGGVP+
Sbjct: 186 KLIGARDYTSEGT---------RDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPA 245
Query: 242 ARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMK 301
+R+A YK+C GCS +L+AFDDAIADGVD+I++S+G A + ND IAIGAFH+M
Sbjct: 246 SRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMA 305
Query: 302 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDP 361
G+LT NSAGN GP ++ +PW L+VAASTT+R V++V +GN G ++N ++
Sbjct: 306 KGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEM 365
Query: 362 LGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDA 421
GK YPL+Y A + + C VD + V GKIL+C + ++
Sbjct: 366 KGKDYPLVYGKSAAS--SACDAESAGLCELSCVDKSRVKGKILVCGG----PGGLKIVES 425
Query: 422 VGVVMNDDGVKYPSNS------YPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVND 481
VG V G+ Y + +PLP++ L T D +++ +Y+ S P A + K++A+ +
Sbjct: 426 VGAV----GLIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFN 485
Query: 482 SNAPFIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISG 541
+P I SFSSRGPN +DILKPD+TAPGVEILAA+SP S D+R Y+++SG
Sbjct: 486 RTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSG 545
Query: 542 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEIN--VEAEFAYGAGQINPL 601
TSMSCPH A YVKTF+P WSP+ I+SA+MTTA P+ EFAYG+G ++P+
Sbjct: 546 TSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPI 605
Query: 602 KAISPGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSF-ALS 661
A +PGLVY+ ++ D++ FLCG YTS +++ +S + C+ A +LNYPS A
Sbjct: 606 AASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKL 665
Query: 662 STPSQSINQFFTRTLTSVDSNASTYTSTIL-GAPQGLTITVNPKVLLFSGIGEKKTFTLT 721
S + F RTLT+V + STYTS ++ G L + + P VL F + EK++FT+T
Sbjct: 666 SGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVT 725
Query: 722 IQGT-IDPTTIVSASLVWSDSSHDVRSPITIY 740
+ G+ +D SA+L+WSD +H+VRSPI +Y
Sbjct: 726 VTGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728
BLAST of CSPI05G10020 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 607.4 bits (1565), Expect = 2.1e-172
Identity = 335/751 (44.61%), Postives = 475/751 (63.25%), Query Frame = 0
Query: 2 SSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTP-SHHMRMLRE 61
+S+ ++ LVL+L VS+++ ++D ++YIVYMG+ P S HM +L++
Sbjct: 6 ASTCLYSWLLVLLLSSVSAII-------DEDTQVYIVYMGSLSSRADYIPTSDHMSILQQ 65
Query: 62 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 121
VTG + L+ SYKRSFNGF +LTE E I+ EGVVSVFP+ LHTT SWDF+
Sbjct: 66 VTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFM 125
Query: 122 GFT--KDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNK 181
G K+ R +ESD ++GV+D+GIWPE+ SFSD G+GP P KWKG+C NFTCN
Sbjct: 126 GVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNN 185
Query: 182 KIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPS 241
K+IGAR Y S+ RD++GHGTHTAST AG V S +G+ GT RGGVP+
Sbjct: 186 KLIGARDYTSEGT---------RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPA 245
Query: 242 ARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMK 301
+RIA YK+C GCS +L++FDDAIADGVD+I++S+G + +D IAIGAFH+M
Sbjct: 246 SRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMA 305
Query: 302 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDP 361
GILT +SAGN GP T+ + +PW +VAASTT+R +++V +GN G ++N FD
Sbjct: 306 KGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDM 365
Query: 362 LGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDA 421
GK+YPL+Y A + + C+ ++ + V GKIL+C PS + + +
Sbjct: 366 KGKKYPLVYGKSAAS--SACDAKTAALCAPACLNKSRVKGKILVCG---GPSGY-KIAKS 425
Query: 422 VGVVMNDDGVKYPSNSYP--LPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAP 481
VG + D P ++ LP+S L+ D ++ +Y+ S P A + K++ + + +P
Sbjct: 426 VGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSP 485
Query: 482 FIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMS 541
I SFSSRGPN +DILKPD+TAPGVEILAA+SP S D+R Y++ SGTSM+
Sbjct: 486 VIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMA 545
Query: 542 CPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEIN--VEAEFAYGAGQINPLKAIS 601
CPH A YVKTF+P WSP+ I+SA+MTTA P+K + EFAYGAG ++P+ A++
Sbjct: 546 CPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALN 605
Query: 602 PGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFA--LSSTP 661
PGLVY+ ++ D++ FLCG YTS ++ +S D C+ N +LNYPS + LS T
Sbjct: 606 PGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTD 665
Query: 662 SQSINQFFTRTLTSVDSNASTYTSTIL-GAPQGLTITVNPKVLLFSGIGEKKTFTLTIQG 721
S + + F RTLT+V + STY S ++ G L+I V P VL F + EK++F++T+ G
Sbjct: 666 S-TFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTG 725
Query: 722 T-IDPTTIVSASLVWSDSSHDVRSPITIYVV 742
+ +D SA+L+WSD +H+VRSPI +Y++
Sbjct: 726 SDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 731
BLAST of CSPI05G10020 vs. ExPASy TrEMBL
Match:
A0A0A0KLY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187860 PE=3 SV=1)
HSP 1 Score: 1467.6 bits (3798), Expect = 0.0e+00
Identity = 739/742 (99.60%), Postives = 740/742 (99.73%), Query Frame = 0
Query: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLRE 60
MSSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLRE
Sbjct: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLRE 60
Query: 61 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 120
VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI
Sbjct: 61 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 120
Query: 121 GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKI 180
GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKI
Sbjct: 121 GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKI 180
Query: 181 IGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR 240
IGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR
Sbjct: 181 IGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR 240
Query: 241 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG 300
IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG
Sbjct: 241 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG 300
Query: 301 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG 360
ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG
Sbjct: 301 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG 360
Query: 361 KQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVG 420
KQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVG
Sbjct: 361 KQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVG 420
Query: 421 VVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIVS 480
VVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDS+APFIVS
Sbjct: 421 VVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSSAPFIVS 480
Query: 481 FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHA 540
FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPH
Sbjct: 481 FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHV 540
Query: 541 TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYD 600
TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYD
Sbjct: 541 TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYD 600
Query: 601 ANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFF 660
ANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFF
Sbjct: 601 ANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFF 660
Query: 661 TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGIGEKKTFTLTIQGTIDPTTIVS 720
TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVL FSGIGEKKTFTLTIQGTIDPTTIVS
Sbjct: 661 TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGIGEKKTFTLTIQGTIDPTTIVS 720
Query: 721 ASLVWSDSSHDVRSPITIYVVT 743
ASLVWSDSSHDVRSPITIYVVT
Sbjct: 721 ASLVWSDSSHDVRSPITIYVVT 742
BLAST of CSPI05G10020 vs. ExPASy TrEMBL
Match:
A0A1S3CF99 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500258 PE=3 SV=1)
HSP 1 Score: 1412.1 bits (3654), Expect = 0.0e+00
Identity = 709/742 (95.55%), Postives = 724/742 (97.57%), Query Frame = 0
Query: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLRE 60
MSSSLIFKLALVLVLGLV SLLASG DS+N DRKIYIVYMGNKPQDTASTPSHHMRMLRE
Sbjct: 14 MSSSLIFKLALVLVLGLVFSLLASGLDSENVDRKIYIVYMGNKPQDTASTPSHHMRMLRE 73
Query: 61 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 120
VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDF+
Sbjct: 74 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFL 133
Query: 121 GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKI 180
GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSD GYGPIPAKWKG CQ NFTCNKKI
Sbjct: 134 GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDVGYGPIPAKWKGSCQTSANFTCNKKI 193
Query: 181 IGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR 240
IGARAYRSDNVFPT DIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR
Sbjct: 194 IGARAYRSDNVFPTADIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR 253
Query: 241 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG 300
IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG
Sbjct: 254 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG 313
Query: 301 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG 360
ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG
Sbjct: 314 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG 373
Query: 361 KQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVG 420
KQYPLIYAGDAPN+ GGFTGSISRFCSEGSVDANLVSGKILLC SI+APSAFVYFS+AVG
Sbjct: 374 KQYPLIYAGDAPNIDGGFTGSISRFCSEGSVDANLVSGKILLCVSIVAPSAFVYFSEAVG 433
Query: 421 VVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIVS 480
VVMNDDGVKYPSNSYPLPSSYL+TVDGDAIKTYMASNGVPTATIFKSDAVNDS APF+VS
Sbjct: 434 VVMNDDGVKYPSNSYPLPSSYLQTVDGDAIKTYMASNGVPTATIFKSDAVNDSYAPFVVS 493
Query: 481 FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHA 540
FSSRGPNPETLDILKPDLTAPG EILA WSPIAPVSSGVIDSRTT+YNIISGTSMSCPHA
Sbjct: 494 FSSRGPNPETLDILKPDLTAPGAEILAGWSPIAPVSSGVIDSRTTMYNIISGTSMSCPHA 553
Query: 541 TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYD 600
TAAAVYVKTFHPTWSPAAIKSALMTTATPLKP+INVEAEFAYGAGQINPLKAI+PGLVYD
Sbjct: 554 TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPDINVEAEFAYGAGQINPLKAINPGLVYD 613
Query: 601 ANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFF 660
ANE DYVKFLCGQGYTS MVQSLSNDNT+CNSANIGRVWDLNYPSFALSSTPSQSINQFF
Sbjct: 614 ANESDYVKFLCGQGYTSAMVQSLSNDNTLCNSANIGRVWDLNYPSFALSSTPSQSINQFF 673
Query: 661 TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGIGEKKTFTLTIQGTIDPTTIVS 720
TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVL FSG+GEK TFTLTIQG+IDPT+IVS
Sbjct: 674 TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGMGEKNTFTLTIQGSIDPTSIVS 733
Query: 721 ASLVWSDSSHDVRSPITIYVVT 743
ASLVWSDSSHDVRSPITIYVV+
Sbjct: 734 ASLVWSDSSHDVRSPITIYVVS 755
BLAST of CSPI05G10020 vs. ExPASy TrEMBL
Match:
A0A5D3E3S0 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold71G00090 PE=3 SV=1)
HSP 1 Score: 1357.4 bits (3512), Expect = 0.0e+00
Identity = 675/703 (96.02%), Postives = 689/703 (98.01%), Query Frame = 0
Query: 40 MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG 99
MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG
Sbjct: 1 MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG 60
Query: 100 VVSVFPSGKKHLHTTRSWDFIGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGP 159
VVSVFPSGKKHLHTTRSWDF+GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSD GYGP
Sbjct: 61 VVSVFPSGKKHLHTTRSWDFLGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDVGYGP 120
Query: 160 IPAKWKGICQNPTNFTCNKKIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLV 219
IPAKWKG CQ NFTCNKKIIGARAYRSDNVFPT DIPSPRDSNGHGTHTASTVAGGLV
Sbjct: 121 IPAKWKGSCQTSANFTCNKKIIGARAYRSDNVFPTADIPSPRDSNGHGTHTASTVAGGLV 180
Query: 220 SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS 279
SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS
Sbjct: 181 SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS 240
Query: 280 EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR 339
EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR
Sbjct: 241 EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR 300
Query: 340 VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGK 399
VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPN+ GGFTGSISRFCSEGSVDANLVSGK
Sbjct: 301 VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPNIDGGFTGSISRFCSEGSVDANLVSGK 360
Query: 400 ILLCDSILAPSAFVYFSDAVGVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGV 459
ILLCDSI+APSAFVYFS+AVGVVMNDDGVKYPSNSYPLPSSYL+TVDGDAIKTYMASNGV
Sbjct: 361 ILLCDSIVAPSAFVYFSEAVGVVMNDDGVKYPSNSYPLPSSYLQTVDGDAIKTYMASNGV 420
Query: 460 PTATIFKSDAVNDSNAPFIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGV 519
PTATIFKSDAVNDS APF+VSFSSRGPNPETLDILKPDLTAPG EILA WSPIAPVSSGV
Sbjct: 421 PTATIFKSDAVNDSYAPFVVSFSSRGPNPETLDILKPDLTAPGAEILAGWSPIAPVSSGV 480
Query: 520 IDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAE 579
IDSRTT+YNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKP+INVEAE
Sbjct: 481 IDSRTTMYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPDINVEAE 540
Query: 580 FAYGAGQINPLKAISPGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVW 639
FAYGAGQINPLKAI+PGLVYDANE DYVKFLCGQGYTS MVQSLSNDNT+CNSANIGRVW
Sbjct: 541 FAYGAGQINPLKAINPGLVYDANESDYVKFLCGQGYTSAMVQSLSNDNTLCNSANIGRVW 600
Query: 640 DLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGI 699
DLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVL FSG+
Sbjct: 601 DLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGM 660
Query: 700 GEKKTFTLTIQGTIDPTTIVSASLVWSDSSHDVRSPITIYVVT 743
GEK TFTLTIQG+IDPT+IVSASLVWSDSSHDVRSPITIYVV+
Sbjct: 661 GEKNTFTLTIQGSIDPTSIVSASLVWSDSSHDVRSPITIYVVS 703
BLAST of CSPI05G10020 vs. ExPASy TrEMBL
Match:
A0A6J1IQ27 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1)
HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 589/742 (79.38%), Postives = 654/742 (88.14%), Query Frame = 0
Query: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSK-NDDRKIYIVYMGNKPQDTASTPSHHMRMLR 60
MSSSLIFK LV+ + SLLAS DS ND RK+YIVY+GNKP+D+ASTPSHHMRML
Sbjct: 1 MSSSLIFK--LVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLE 60
Query: 61 EVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDF 120
EV GS FAP++LLHSYKRSFNGFVVKLTEEEA +ISAKEGVVSVFP+GKKHLHTTRSWDF
Sbjct: 61 EVVGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDF 120
Query: 121 IGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKK 180
+GFTK V RV QVES+IVVGVLDSGIWPE+PSFSD GYGP PAKWKG CQ NF CN+K
Sbjct: 121 MGFTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRK 180
Query: 181 IIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSA 240
IIGARAYRSDN+FP EDI SPRDS+GHGTHTASTVAGGLV+QASLYGLALGTARGGVPSA
Sbjct: 181 IIGARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSA 240
Query: 241 RIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKH 300
RIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG++ +YYFNDSIAIGAFHSMKH
Sbjct: 241 RIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 300
Query: 301 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPL 360
GILTSNSAGNDGPDYFTIRNFSPWSLSVAAS+ DRKLVS+V++GN NVYQGYTINTFD
Sbjct: 301 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 360
Query: 361 GKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAV 420
GKQYPLIYAG+APN+ GGFTGS SRFCS SVD NLV GKILLCDSIL+PS F F+ AV
Sbjct: 361 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 420
Query: 421 GVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIV 480
GVVMND GVK S SYPLPSSYL V G+ IKTYM SN PTATIFKS+AVND++AP IV
Sbjct: 421 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 480
Query: 481 SFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPH 540
SFSSRGPNPET DILKPDLTAPGVEILAAWSPIA VSSGV DSRTTLYNIISGTSMSCPH
Sbjct: 481 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 540
Query: 541 ATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVY 600
ATAAAVYVKTFHP+WSPAAIKSALMTTA PL P++N +AEFAYGAG INP+KA++PGLVY
Sbjct: 541 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVY 600
Query: 601 DANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQF 660
DA E DYV+FLCGQGYT+ MV+ LS D+++C AN GRVWDLNYPSFALSSTPS+SINQF
Sbjct: 601 DAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQF 660
Query: 661 FTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGIGEKKTFTLTIQGTIDPTTIV 720
F RT+T+V S +TY + +LGAP+GLTI+VNP L F+ IG+KK+FT+T++G ++ IV
Sbjct: 661 FRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVN-QPIV 720
Query: 721 SASLVWSDSSHDVRSPITIYVV 742
SA+L+W+D H VRSPIT+YVV
Sbjct: 721 SAALLWTDGHHHVRSPITVYVV 739
BLAST of CSPI05G10020 vs. ExPASy TrEMBL
Match:
A0A6J1FV97 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1)
HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 588/742 (79.25%), Postives = 655/742 (88.27%), Query Frame = 0
Query: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSK-NDDRKIYIVYMGNKPQDTASTPSHHMRMLR 60
MSSSLIFK LV+ + SLLASG DS ND RK+YIVY+GNKP+D+ASTPSHHMRML
Sbjct: 1 MSSSLIFK--LVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLE 60
Query: 61 EVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDF 120
EV GS FAP++LLHSYKRSFNGFVVKLTEEEA +ISAKEGVVSVFP+GKKHLHTTRSWDF
Sbjct: 61 EVVGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDF 120
Query: 121 IGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKK 180
+GFTK+V RV QVES+IVVGVLDSGIWPE+PSFSD GYGP PAKWKG CQ NF CN+K
Sbjct: 121 MGFTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRK 180
Query: 181 IIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSA 240
IIGARAYRSDN FP EDI SPRDS+GHGTHTASTVAGGLV+QASLYGLALGTARGGVPSA
Sbjct: 181 IIGARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSA 240
Query: 241 RIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKH 300
RIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG++ +YYFNDSIAIGAFHSMKH
Sbjct: 241 RIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 300
Query: 301 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPL 360
GILTSNSAGNDGPDYFTIRNFSPWSLSVAAS+ DRKLVS+V++GN NVYQGYTINTFD
Sbjct: 301 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 360
Query: 361 GKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAV 420
GKQYPLIYAG+APN+ GGFTGS SRFCS SVD NLV GKILLCDSIL+PS F F+ AV
Sbjct: 361 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 420
Query: 421 GVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIV 480
GVVMND GVK + SYPLPSSYL V G+ IKTYM S+ PTATIFKS+AVND++AP IV
Sbjct: 421 GVVMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIV 480
Query: 481 SFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPH 540
SFSSRGPNPET DILKPDLTAPGVEILAAWSPIA VSSGV DSRTTLYNIISGTSMSCPH
Sbjct: 481 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 540
Query: 541 ATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVY 600
ATAAAVYVKTFHP+WSPAAIKSALMTTA PL P++N +AEFAYGAG INP+KA++PGLVY
Sbjct: 541 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVY 600
Query: 601 DANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQF 660
DA E DYV+FLCGQGYT+ MV+ LS D+++C AN GRVWDLNYPSFALSSTPS+SINQF
Sbjct: 601 DAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQF 660
Query: 661 FTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGIGEKKTFTLTIQGTIDPTTIV 720
F RT+T+V S +TY + +LG P+GLTI+VNP VL F+ IG+KK+FT+T++G ++ IV
Sbjct: 661 FRRTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVN-QPIV 720
Query: 721 SASLVWSDSSHDVRSPITIYVV 742
SA+L+W+D H VRSPIT+YVV
Sbjct: 721 SAALLWTDGHHHVRSPITVYVV 739
BLAST of CSPI05G10020 vs. NCBI nr
Match:
XP_011656180.2 (cucumisin [Cucumis sativus] >KAE8648002.1 hypothetical protein Csa_021416 [Cucumis sativus])
HSP 1 Score: 1469.1 bits (3802), Expect = 0.0e+00
Identity = 740/742 (99.73%), Postives = 741/742 (99.87%), Query Frame = 0
Query: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLRE 60
MSSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLRE
Sbjct: 6 MSSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLRE 65
Query: 61 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 120
VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI
Sbjct: 66 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 125
Query: 121 GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKI 180
GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKI
Sbjct: 126 GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKI 185
Query: 181 IGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR 240
IGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR
Sbjct: 186 IGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR 245
Query: 241 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG 300
IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG
Sbjct: 246 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG 305
Query: 301 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG 360
ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG
Sbjct: 306 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG 365
Query: 361 KQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVG 420
KQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVG
Sbjct: 366 KQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVG 425
Query: 421 VVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIVS 480
VVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDS+APFIVS
Sbjct: 426 VVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSSAPFIVS 485
Query: 481 FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHA 540
FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHA
Sbjct: 486 FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHA 545
Query: 541 TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYD 600
TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYD
Sbjct: 546 TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYD 605
Query: 601 ANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFF 660
ANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFF
Sbjct: 606 ANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFF 665
Query: 661 TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGIGEKKTFTLTIQGTIDPTTIVS 720
TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVL FSGIGEKKTFTLTIQGTIDPTTIVS
Sbjct: 666 TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGIGEKKTFTLTIQGTIDPTTIVS 725
Query: 721 ASLVWSDSSHDVRSPITIYVVT 743
ASLVWSDSSHDVRSPITIYVVT
Sbjct: 726 ASLVWSDSSHDVRSPITIYVVT 747
BLAST of CSPI05G10020 vs. NCBI nr
Match:
XP_008461725.1 (PREDICTED: cucumisin-like [Cucumis melo])
HSP 1 Score: 1412.1 bits (3654), Expect = 0.0e+00
Identity = 709/742 (95.55%), Postives = 724/742 (97.57%), Query Frame = 0
Query: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLRE 60
MSSSLIFKLALVLVLGLV SLLASG DS+N DRKIYIVYMGNKPQDTASTPSHHMRMLRE
Sbjct: 14 MSSSLIFKLALVLVLGLVFSLLASGLDSENVDRKIYIVYMGNKPQDTASTPSHHMRMLRE 73
Query: 61 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 120
VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDF+
Sbjct: 74 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFL 133
Query: 121 GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKI 180
GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSD GYGPIPAKWKG CQ NFTCNKKI
Sbjct: 134 GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDVGYGPIPAKWKGSCQTSANFTCNKKI 193
Query: 181 IGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR 240
IGARAYRSDNVFPT DIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR
Sbjct: 194 IGARAYRSDNVFPTADIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR 253
Query: 241 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG 300
IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG
Sbjct: 254 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG 313
Query: 301 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG 360
ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG
Sbjct: 314 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG 373
Query: 361 KQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVG 420
KQYPLIYAGDAPN+ GGFTGSISRFCSEGSVDANLVSGKILLC SI+APSAFVYFS+AVG
Sbjct: 374 KQYPLIYAGDAPNIDGGFTGSISRFCSEGSVDANLVSGKILLCVSIVAPSAFVYFSEAVG 433
Query: 421 VVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIVS 480
VVMNDDGVKYPSNSYPLPSSYL+TVDGDAIKTYMASNGVPTATIFKSDAVNDS APF+VS
Sbjct: 434 VVMNDDGVKYPSNSYPLPSSYLQTVDGDAIKTYMASNGVPTATIFKSDAVNDSYAPFVVS 493
Query: 481 FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHA 540
FSSRGPNPETLDILKPDLTAPG EILA WSPIAPVSSGVIDSRTT+YNIISGTSMSCPHA
Sbjct: 494 FSSRGPNPETLDILKPDLTAPGAEILAGWSPIAPVSSGVIDSRTTMYNIISGTSMSCPHA 553
Query: 541 TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYD 600
TAAAVYVKTFHPTWSPAAIKSALMTTATPLKP+INVEAEFAYGAGQINPLKAI+PGLVYD
Sbjct: 554 TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPDINVEAEFAYGAGQINPLKAINPGLVYD 613
Query: 601 ANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFF 660
ANE DYVKFLCGQGYTS MVQSLSNDNT+CNSANIGRVWDLNYPSFALSSTPSQSINQFF
Sbjct: 614 ANESDYVKFLCGQGYTSAMVQSLSNDNTLCNSANIGRVWDLNYPSFALSSTPSQSINQFF 673
Query: 661 TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGIGEKKTFTLTIQGTIDPTTIVS 720
TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVL FSG+GEK TFTLTIQG+IDPT+IVS
Sbjct: 674 TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGMGEKNTFTLTIQGSIDPTSIVS 733
Query: 721 ASLVWSDSSHDVRSPITIYVVT 743
ASLVWSDSSHDVRSPITIYVV+
Sbjct: 734 ASLVWSDSSHDVRSPITIYVVS 755
BLAST of CSPI05G10020 vs. NCBI nr
Match:
KAA0051597.1 (cucumisin-like [Cucumis melo var. makuwa] >TYK30544.1 cucumisin-like [Cucumis melo var. makuwa])
HSP 1 Score: 1357.4 bits (3512), Expect = 0.0e+00
Identity = 675/703 (96.02%), Postives = 689/703 (98.01%), Query Frame = 0
Query: 40 MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG 99
MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG
Sbjct: 1 MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG 60
Query: 100 VVSVFPSGKKHLHTTRSWDFIGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGP 159
VVSVFPSGKKHLHTTRSWDF+GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSD GYGP
Sbjct: 61 VVSVFPSGKKHLHTTRSWDFLGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDVGYGP 120
Query: 160 IPAKWKGICQNPTNFTCNKKIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLV 219
IPAKWKG CQ NFTCNKKIIGARAYRSDNVFPT DIPSPRDSNGHGTHTASTVAGGLV
Sbjct: 121 IPAKWKGSCQTSANFTCNKKIIGARAYRSDNVFPTADIPSPRDSNGHGTHTASTVAGGLV 180
Query: 220 SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS 279
SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS
Sbjct: 181 SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS 240
Query: 280 EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR 339
EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR
Sbjct: 241 EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR 300
Query: 340 VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGK 399
VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPN+ GGFTGSISRFCSEGSVDANLVSGK
Sbjct: 301 VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPNIDGGFTGSISRFCSEGSVDANLVSGK 360
Query: 400 ILLCDSILAPSAFVYFSDAVGVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGV 459
ILLCDSI+APSAFVYFS+AVGVVMNDDGVKYPSNSYPLPSSYL+TVDGDAIKTYMASNGV
Sbjct: 361 ILLCDSIVAPSAFVYFSEAVGVVMNDDGVKYPSNSYPLPSSYLQTVDGDAIKTYMASNGV 420
Query: 460 PTATIFKSDAVNDSNAPFIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGV 519
PTATIFKSDAVNDS APF+VSFSSRGPNPETLDILKPDLTAPG EILA WSPIAPVSSGV
Sbjct: 421 PTATIFKSDAVNDSYAPFVVSFSSRGPNPETLDILKPDLTAPGAEILAGWSPIAPVSSGV 480
Query: 520 IDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAE 579
IDSRTT+YNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKP+INVEAE
Sbjct: 481 IDSRTTMYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPDINVEAE 540
Query: 580 FAYGAGQINPLKAISPGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVW 639
FAYGAGQINPLKAI+PGLVYDANE DYVKFLCGQGYTS MVQSLSNDNT+CNSANIGRVW
Sbjct: 541 FAYGAGQINPLKAINPGLVYDANESDYVKFLCGQGYTSAMVQSLSNDNTLCNSANIGRVW 600
Query: 640 DLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGI 699
DLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVL FSG+
Sbjct: 601 DLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGM 660
Query: 700 GEKKTFTLTIQGTIDPTTIVSASLVWSDSSHDVRSPITIYVVT 743
GEK TFTLTIQG+IDPT+IVSASLVWSDSSHDVRSPITIYVV+
Sbjct: 661 GEKNTFTLTIQGSIDPTSIVSASLVWSDSSHDVRSPITIYVVS 703
BLAST of CSPI05G10020 vs. NCBI nr
Match:
XP_038892474.1 (cucumisin-like [Benincasa hispida])
HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 660/742 (88.95%), Postives = 700/742 (94.34%), Query Frame = 0
Query: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLRE 60
MSSSLIFK LV+VL LVSSLL S DS D RK+YIVYMGNKP+DTASTPSHHMRML+E
Sbjct: 6 MSSSLIFK--LVVVLSLVSSLLVSSSDSDKDGRKVYIVYMGNKPKDTASTPSHHMRMLKE 65
Query: 61 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 120
VTGSNFAPESLLHSYKRSFNGFVVKLTEEEA ISAKEGVVSVFP+GKKHLHTTRSWDFI
Sbjct: 66 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEARTISAKEGVVSVFPNGKKHLHTTRSWDFI 125
Query: 121 GFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKI 180
GFTKDVPRV QVES+IVVGVLDSGIWPENPSFSD GYGP PAKWKG CQ TNFTCN KI
Sbjct: 126 GFTKDVPRVKQVESNIVVGVLDSGIWPENPSFSDFGYGPPPAKWKGTCQTSTNFTCNNKI 185
Query: 181 IGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSAR 240
IGARAYRSDN FPT+DI SPRDS+GHGTHTASTVAGGLV+QASLYGLALGTARGGVPSAR
Sbjct: 186 IGARAYRSDNDFPTQDIQSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSAR 245
Query: 241 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHG 300
IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGG+EARYYFNDSIAIGAFHSMKHG
Sbjct: 246 IAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGNEARYYFNDSIAIGAFHSMKHG 305
Query: 301 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLG 360
ILTSNSAGNDGPDYFTIRNFSPWSLSVAAST DRKLVSRV++GN N+YQGYTINTFD G
Sbjct: 306 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSRVQLGNRNIYQGYTINTFDLGG 365
Query: 361 KQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVG 420
KQYPLIYAGDAPN+ GGFTGSISRFCSE S+D++LVSGKILLCDS+L PS+FVYFSDA G
Sbjct: 366 KQYPLIYAGDAPNIAGGFTGSISRFCSENSIDSSLVSGKILLCDSVLPPSSFVYFSDAAG 425
Query: 421 VVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIVS 480
VVMND+GVK PSNSYPLPSSYLETVDG+AIKTYMASNGVPTATI KS AVND++APFIVS
Sbjct: 426 VVMNDNGVKNPSNSYPLPSSYLETVDGNAIKTYMASNGVPTATILKSSAVNDTSAPFIVS 485
Query: 481 FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHA 540
FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAP+SSGVIDSRT++YNIISGTSMSCPHA
Sbjct: 486 FSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPISSGVIDSRTSMYNIISGTSMSCPHA 545
Query: 541 TAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYD 600
TAAAVYVKTFHPTWSPAAIKSALMTTATPL PE+NV+AEFAYGAGQINPLKAISPGLVYD
Sbjct: 546 TAAAVYVKTFHPTWSPAAIKSALMTTATPLNPELNVQAEFAYGAGQINPLKAISPGLVYD 605
Query: 601 ANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFF 660
ANEFDYVKFLCGQGYT+ MVQ LSNDN+IC+SAN GRVWDLNYPSFALSSTPSQSINQFF
Sbjct: 606 ANEFDYVKFLCGQGYTTAMVQRLSNDNSICSSANSGRVWDLNYPSFALSSTPSQSINQFF 665
Query: 661 TRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGIGEKKTFTLTIQGTIDPTTIVS 720
RTLT+V+S ASTY STILGAPQGLTITVNP+VL FSG GEKK+FTLTI+GTI+P +IVS
Sbjct: 666 RRTLTNVESKASTYRSTILGAPQGLTITVNPQVLSFSGFGEKKSFTLTIRGTINPPSIVS 725
Query: 721 ASLVWSDSSHDVRSPITIYVVT 743
ASLVWSD SH+VRSPIT+YVVT
Sbjct: 726 ASLVWSDGSHNVRSPITVYVVT 745
BLAST of CSPI05G10020 vs. NCBI nr
Match:
XP_022979186.1 (cucumisin-like [Cucurbita maxima])
HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 589/742 (79.38%), Postives = 654/742 (88.14%), Query Frame = 0
Query: 1 MSSSLIFKLALVLVLGLVSSLLASGFDSK-NDDRKIYIVYMGNKPQDTASTPSHHMRMLR 60
MSSSLIFK LV+ + SLLAS DS ND RK+YIVY+GNKP+D+ASTPSHHMRML
Sbjct: 1 MSSSLIFK--LVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLE 60
Query: 61 EVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDF 120
EV GS FAP++LLHSYKRSFNGFVVKLTEEEA +ISAKEGVVSVFP+GKKHLHTTRSWDF
Sbjct: 61 EVVGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDF 120
Query: 121 IGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKK 180
+GFTK V RV QVES+IVVGVLDSGIWPE+PSFSD GYGP PAKWKG CQ NF CN+K
Sbjct: 121 MGFTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRK 180
Query: 181 IIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSA 240
IIGARAYRSDN+FP EDI SPRDS+GHGTHTASTVAGGLV+QASLYGLALGTARGGVPSA
Sbjct: 181 IIGARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSA 240
Query: 241 RIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKH 300
RIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG++ +YYFNDSIAIGAFHSMKH
Sbjct: 241 RIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKH 300
Query: 301 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPL 360
GILTSNSAGNDGPDYFTIRNFSPWSLSVAAS+ DRKLVS+V++GN NVYQGYTINTFD
Sbjct: 301 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLK 360
Query: 361 GKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAV 420
GKQYPLIYAG+APN+ GGFTGS SRFCS SVD NLV GKILLCDSIL+PS F F+ AV
Sbjct: 361 GKQYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAV 420
Query: 421 GVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIV 480
GVVMND GVK S SYPLPSSYL V G+ IKTYM SN PTATIFKS+AVND++AP IV
Sbjct: 421 GVVMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIV 480
Query: 481 SFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPH 540
SFSSRGPNPET DILKPDLTAPGVEILAAWSPIA VSSGV DSRTTLYNIISGTSMSCPH
Sbjct: 481 SFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPH 540
Query: 541 ATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVY 600
ATAAAVYVKTFHP+WSPAAIKSALMTTA PL P++N +AEFAYGAG INP+KA++PGLVY
Sbjct: 541 ATAAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVY 600
Query: 601 DANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQF 660
DA E DYV+FLCGQGYT+ MV+ LS D+++C AN GRVWDLNYPSFALSSTPS+SINQF
Sbjct: 601 DAQESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQF 660
Query: 661 FTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFSGIGEKKTFTLTIQGTIDPTTIV 720
F RT+T+V S +TY + +LGAP+GLTI+VNP L F+ IG+KK+FT+T++G ++ IV
Sbjct: 661 FRRTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVN-QPIV 720
Query: 721 SASLVWSDSSHDVRSPITIYVV 742
SA+L+W+D H VRSPIT+YVV
Sbjct: 721 SAALLWTDGHHHVRSPITVYVV 739
BLAST of CSPI05G10020 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 632.9 bits (1631), Expect = 3.3e-181
Identity = 335/705 (47.52%), Postives = 456/705 (64.68%), Query Frame = 0
Query: 40 MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG 99
MG P+ S PSHH+ +L+++ G+ A L+ SYKRSFNGF L++ E+ ++ +
Sbjct: 1 MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60
Query: 100 VVSVFPSGKKHLHTTRSWDFIGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGP 159
VVSVFPS L TTRSWDF+GF + R + ESD++VGV+DSGIWPE+ SF D G+GP
Sbjct: 61 VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120
Query: 160 IPAKWKGICQNPTNFTCNKKIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLV 219
P KWKG C+ F CN K+IGAR Y N F S RD GHGTHTAST AG V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFY---NKF----ADSARDEEGHGTHTASTAAGNAV 180
Query: 220 SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS 279
AS YGLA GTARGGVPSARIA YK+C+ + C+D DILAAFDDAIADGVD+IS+S+
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240
Query: 280 EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR 339
N S+AIG+FH+M GI+T+ SAGN+GPD ++ N SPW ++VAAS TDR+ + R
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300
Query: 340 VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGK 399
V +GN G ++NTF+ G ++P++Y N+ + + + +CS G VD+ LV GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360
Query: 400 ILLCDSILAPSAFVYFSDAVGVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGV 459
I+LCD L Y + A+GV++ + + + P P+S L D +IK+Y+ S
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420
Query: 460 PTATIFKSDAVNDSNAPFIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSS-- 519
P A I +++ + D AP++ SFSSRGP+ ++LKPD++APG+EILAA+SP+A SS
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480
Query: 520 GVIDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVE 579
D R+ Y+++SGTSM+CPH A YVK+FHP WSP+AIKSA+MTTATP+ + N E
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540
Query: 580 AEFAYGAGQINPLKAISPGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGR 639
EFAYG+GQINP KA PGLVY+ DY+K LC +G+ S + + S N C+
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600
Query: 640 VWDLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLLFS 699
V DLNYP+ + N F RT+T+V STY ++++ L I++ P++L F
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660
Query: 700 GIGEKKTFTLTIQG-TIDPTTIVSASLVWSDSSHDVRSPITIYVV 742
+ EKK+F +TI G + + VS+S+VWSD SH VRSPI Y +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691
BLAST of CSPI05G10020 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 610.9 bits (1574), Expect = 1.3e-174
Identity = 331/750 (44.13%), Postives = 475/750 (63.33%), Query Frame = 0
Query: 3 SSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTP-SHHMRMLREV 62
++ IF + +LVL L S S + D+++YIVY+G+ P TP S HM +L+E+
Sbjct: 5 TTFIFLFSSLLVLSLSS---VSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEI 64
Query: 63 TGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFIG 122
TG + L+ SYK+SFNGF +LTE E R++ E VVSVFPS K L TT SW+F+G
Sbjct: 65 TGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMG 124
Query: 123 FTKDV--PRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKK 182
+ + R +ESD ++GV+DSGI+PE+ SFSD G+GP P KWKG C NFTCN K
Sbjct: 125 LKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNK 184
Query: 183 IIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSA 242
+IGAR Y + ++ + RD +GHGTHTAS AG V+ ++ YGL GTARGGVP+A
Sbjct: 185 VIGARDYTA----KSKANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAA 244
Query: 243 RIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKH 302
RIAVYK+C ++GC +++AFDDAIADGVD+IS+S+ + D IAIGAFH+M
Sbjct: 245 RIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAV 304
Query: 303 GILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPL 362
G+LT N+AGN+GP T+ + +PW SVAAS T+R +++V +G+ + G ++NT+D
Sbjct: 305 GVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMN 364
Query: 363 GKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAV 422
G YPL+Y A + + +R C +D LV GKI+LCDS
Sbjct: 365 GTNYPLVYGKSA--ALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVG 424
Query: 423 GVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAPFIV 482
+V N + + S+P+ S+L D ++ +YM S P AT+ KS+ +++ AP +
Sbjct: 425 SIVKNPEPDRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVA 484
Query: 483 SFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPH 542
SFSSRGP+ DILKPD+TAPGVEILAA+SP + + D+R Y+++SGTSM+CPH
Sbjct: 485 SFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPH 544
Query: 543 ATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEIN--VEAEFAYGAGQINPLKAISPGL 602
A YVKTFHP WSP+ I+SA+MTTA P+ + V EFAYG+G ++P+ AI+PGL
Sbjct: 545 VAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGL 604
Query: 603 VYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICN---SANIGRVWDLNYPSFALSSTPSQ 662
VY+ + D++ FLCG YTSD ++ +S DN+ C S + R +LNYP+ + + ++
Sbjct: 605 VYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGTK 664
Query: 663 SINQFFTRTLTSVDSNASTYTSTILGAP-QGLTITVNPKVLLFSGIGEKKTFTLTIQG-T 722
N F RT+T+V STY + ++ P L+I V+P+VL + EK++F +T+ +
Sbjct: 665 PFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDS 724
Query: 723 IDPTTIVSASLVWSDSSHDVRSPITIYVVT 743
I VSA+L+WSD +H+VRSPI +Y ++
Sbjct: 725 IGTKQPVSANLIWSDGTHNVRSPIIVYAMS 741
BLAST of CSPI05G10020 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 608.6 bits (1568), Expect = 6.7e-174
Identity = 339/752 (45.08%), Postives = 466/752 (61.97%), Query Frame = 0
Query: 2 SSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTP-SHHMRMLRE 61
+SS + LVL L VS++ DD+++YIVYMG+ TP S HM +L+E
Sbjct: 6 ASSSLLSCLLVLFLSSVSAV--------TDDKQVYIVYMGSLSSRADYTPTSDHMNILQE 65
Query: 62 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 121
VTG + L+ SYKRSFNGF +LTE E R++ GVVSVFP+ K L TT SWDF+
Sbjct: 66 VTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFM 125
Query: 122 GFTKDV--PRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNK 181
G + + R VESD ++GV+DSGI PE+ SFSD G+GP P KWKG+C NFTCN
Sbjct: 126 GLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNN 185
Query: 182 KIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPS 241
K+IGAR Y S+ RD +GHGTHTAST AG V AS +G+ GT RGGVP+
Sbjct: 186 KLIGARDYTSEGT---------RDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPA 245
Query: 242 ARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMK 301
+R+A YK+C GCS +L+AFDDAIADGVD+I++S+G A + ND IAIGAFH+M
Sbjct: 246 SRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMA 305
Query: 302 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDP 361
G+LT NSAGN GP ++ +PW L+VAASTT+R V++V +GN G ++N ++
Sbjct: 306 KGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEM 365
Query: 362 LGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDA 421
GK YPL+Y A + + C VD + V GKIL+C + ++
Sbjct: 366 KGKDYPLVYGKSAAS--SACDAESAGLCELSCVDKSRVKGKILVCGG----PGGLKIVES 425
Query: 422 VGVVMNDDGVKYPSNS------YPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVND 481
VG V G+ Y + +PLP++ L T D +++ +Y+ S P A + K++A+ +
Sbjct: 426 VGAV----GLIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFN 485
Query: 482 SNAPFIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISG 541
+P I SFSSRGPN +DILKPD+TAPGVEILAA+SP S D+R Y+++SG
Sbjct: 486 RTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSG 545
Query: 542 TSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEIN--VEAEFAYGAGQINPL 601
TSMSCPH A YVKTF+P WSP+ I+SA+MTTA P+ EFAYG+G ++P+
Sbjct: 546 TSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPI 605
Query: 602 KAISPGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSF-ALS 661
A +PGLVY+ ++ D++ FLCG YTS +++ +S + C+ A +LNYPS A
Sbjct: 606 AASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKL 665
Query: 662 STPSQSINQFFTRTLTSVDSNASTYTSTIL-GAPQGLTITVNPKVLLFSGIGEKKTFTLT 721
S + F RTLT+V + STYTS ++ G L + + P VL F + EK++FT+T
Sbjct: 666 SGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVT 725
Query: 722 IQGT-IDPTTIVSASLVWSDSSHDVRSPITIY 740
+ G+ +D SA+L+WSD +H+VRSPI +Y
Sbjct: 726 VTGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728
BLAST of CSPI05G10020 vs. TAIR 10
Match:
AT5G59090.1 (subtilase 4.12 )
HSP 1 Score: 607.4 bits (1565), Expect = 1.5e-173
Identity = 335/751 (44.61%), Postives = 475/751 (63.25%), Query Frame = 0
Query: 2 SSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTP-SHHMRMLRE 61
+S+ ++ LVL+L VS+++ ++D ++YIVYMG+ P S HM +L++
Sbjct: 6 ASTCLYSWLLVLLLSSVSAII-------DEDTQVYIVYMGSLSSRADYIPTSDHMSILQQ 65
Query: 62 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 121
VTG + L+ SYKRSFNGF +LTE E I+ EGVVSVFP+ LHTT SWDF+
Sbjct: 66 VTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFM 125
Query: 122 GFT--KDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNK 181
G K+ R +ESD ++GV+D+GIWPE+ SFSD G+GP P KWKG+C NFTCN
Sbjct: 126 GVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNN 185
Query: 182 KIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPS 241
K+IGAR Y S+ RD++GHGTHTAST AG V S +G+ GT RGGVP+
Sbjct: 186 KLIGARDYTSEGT---------RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPA 245
Query: 242 ARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMK 301
+RIA YK+C GCS +L++FDDAIADGVD+I++S+G + +D IAIGAFH+M
Sbjct: 246 SRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMA 305
Query: 302 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDP 361
GILT +SAGN GP T+ + +PW +VAASTT+R +++V +GN G ++N FD
Sbjct: 306 KGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDM 365
Query: 362 LGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDA 421
GK+YPL+Y A + + C+ ++ + V GKIL+C PS + + +
Sbjct: 366 KGKKYPLVYGKSAAS--SACDAKTAALCAPACLNKSRVKGKILVCG---GPSGY-KIAKS 425
Query: 422 VGVVMNDDGVKYPSNSYP--LPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAP 481
VG + D P ++ LP+S L+ D ++ +Y+ S P A + K++ + + +P
Sbjct: 426 VGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSP 485
Query: 482 FIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMS 541
I SFSSRGPN +DILKPD+TAPGVEILAA+SP S D+R Y++ SGTSM+
Sbjct: 486 VIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMA 545
Query: 542 CPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEIN--VEAEFAYGAGQINPLKAIS 601
CPH A YVKTF+P WSP+ I+SA+MTTA P+K + EFAYGAG ++P+ A++
Sbjct: 546 CPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALN 605
Query: 602 PGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFA--LSSTP 661
PGLVY+ ++ D++ FLCG YTS ++ +S D C+ N +LNYPS + LS T
Sbjct: 606 PGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTD 665
Query: 662 SQSINQFFTRTLTSVDSNASTYTSTIL-GAPQGLTITVNPKVLLFSGIGEKKTFTLTIQG 721
S + + F RTLT+V + STY S ++ G L+I V P VL F + EK++F++T+ G
Sbjct: 666 S-TFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTG 725
Query: 722 T-IDPTTIVSASLVWSDSSHDVRSPITIYVV 742
+ +D SA+L+WSD +H+VRSPI +Y++
Sbjct: 726 SDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 731
BLAST of CSPI05G10020 vs. TAIR 10
Match:
AT5G59090.3 (subtilase 4.12 )
HSP 1 Score: 604.4 bits (1557), Expect = 1.3e-172
Identity = 335/751 (44.61%), Postives = 474/751 (63.12%), Query Frame = 0
Query: 2 SSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTP-SHHMRMLRE 61
+S+ ++ LVL+L VS+++ ++D ++YIVYMG+ P S HM +L++
Sbjct: 6 ASTCLYSWLLVLLLSSVSAII-------DEDTQVYIVYMGSLSSRADYIPTSDHMSILQQ 65
Query: 62 VTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFI 121
VTG + L+ SYKRSFNGF +LTE E R EGVVSVFP+ LHTT SWDF+
Sbjct: 66 VTGESSIEGRLVRSYKRSFNGFAARLTESE--RTLIAEGVVSVFPNKILQLHTTTSWDFM 125
Query: 122 GFT--KDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNK 181
G K+ R +ESD ++GV+D+GIWPE+ SFSD G+GP P KWKG+C NFTCN
Sbjct: 126 GVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNN 185
Query: 182 KIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPS 241
K+IGAR Y S+ RD++GHGTHTAST AG V S +G+ GT RGGVP+
Sbjct: 186 KLIGARDYTSEGT---------RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPA 245
Query: 242 ARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMK 301
+RIA YK+C GCS +L++FDDAIADGVD+I++S+G + +D IAIGAFH+M
Sbjct: 246 SRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMA 305
Query: 302 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDP 361
GILT +SAGN GP T+ + +PW +VAASTT+R +++V +GN G ++N FD
Sbjct: 306 KGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDM 365
Query: 362 LGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDA 421
GK+YPL+Y A + + C+ ++ + V GKIL+C PS + + +
Sbjct: 366 KGKKYPLVYGKSAAS--SACDAKTAALCAPACLNKSRVKGKILVCG---GPSGY-KIAKS 425
Query: 422 VGVVMNDDGVKYPSNSYP--LPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSNAP 481
VG + D P ++ LP+S L+ D ++ +Y+ S P A + K++ + + +P
Sbjct: 426 VGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSP 485
Query: 482 FIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMS 541
I SFSSRGPN +DILKPD+TAPGVEILAA+SP S D+R Y++ SGTSM+
Sbjct: 486 VIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMA 545
Query: 542 CPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEIN--VEAEFAYGAGQINPLKAIS 601
CPH A YVKTF+P WSP+ I+SA+MTTA P+K + EFAYGAG ++P+ A++
Sbjct: 546 CPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALN 605
Query: 602 PGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFA--LSSTP 661
PGLVY+ ++ D++ FLCG YTS ++ +S D C+ N +LNYPS + LS T
Sbjct: 606 PGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTD 665
Query: 662 SQSINQFFTRTLTSVDSNASTYTSTIL-GAPQGLTITVNPKVLLFSGIGEKKTFTLTIQG 721
S + + F RTLT+V + STY S ++ G L+I V P VL F + EK++F++T+ G
Sbjct: 666 S-TFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTG 725
Query: 722 T-IDPTTIVSASLVWSDSSHDVRSPITIYVV 742
+ +D SA+L+WSD +H+VRSPI +Y++
Sbjct: 726 SDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 729
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 1.7e-254 | 61.84 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q9FIF8 | 1.2e-183 | 47.64 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Q9FGU3 | 1.9e-173 | 44.13 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
| Q9FIG2 | 9.4e-173 | 45.08 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
| Q8L7D2 | 2.1e-172 | 44.61 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KLY4 | 0.0e+00 | 99.60 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187860 PE=3 SV=1 | [more] |
| A0A1S3CF99 | 0.0e+00 | 95.55 | cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500258 PE=3 SV=1 | [more] |
| A0A5D3E3S0 | 0.0e+00 | 96.02 | Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold71G00090 ... | [more] |
| A0A6J1IQ27 | 0.0e+00 | 79.38 | cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1 | [more] |
| A0A6J1FV97 | 0.0e+00 | 79.25 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1 | [more] |
