CSPI05G09230 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G09230
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	Chr5: 7818324 .. 7820722 (+)
RNA-Seq Expression	CSPI05G09230
Synteny	CSPI05G09230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGTAATTTCCCTTCTCTAGTTTTCTCTCGACGGCAATGGAGATTGAGAATGTAAGATATGAAGAGGTAAGTTCTTTTTTTTTTTTTTGAAAATTTAAAATGGGTTTTTTTTTTGTTGGGGGGTTTGCTCGTGAATTAATGGTGTTTTTTGTTTATTCTTTTTGGGCAGGAAGTAATAGTGAATGGACGTGGGTTAAAGTTGTTCACGTGCAATTGGGTTCCAAAGAATGAAGAACCAAAAGCTTTGATTTTTCTCTGCCATGGGTATGCCATGGAATGTAGCATCACAATGGACAGTTAGTATACTTTTTCATAAACTCTTCTTCATAACCAACTATTTGGTTTTATTTTGTTAAAAGGGTTTGGAAATTGAGCATTTCTTTTTCATTTGGTTAGGTAGTGCACGAAGATTAGCAAAAGAAGGATATGGAGTGTATGGAATTGATTATGAAGGACATGGAAAATCCAGTGGATTACAAGGGTATGTTTCAAGTTTTGATAACGTGGTGGACGATTGTTCTTCCTTTTTCACTTCAATATCAGGTAAATATTTTTCATATATACAATTTGTGTTTCATCCTTCCTTTTGGAATTGAAAGCTGATGATGATGATGATGCATAATAATAATAATAATGGAAACAGAAAAGAAAGAAAATAGAGAGAAGAAAAGGTATTTGATGGGAGAGTCAATGGGAGGAGCAGTGGCTCTGATGATACACAGAAAACAGCCTGATTTTTGGGATGGTGCCATCTTGGTTGCTCCTATGTGTAAGGTAACTTTTCCTCTCTCTTTTTCTTTTTTCATTCAAAGTTTCATTCATTTAACAAAATTCCAACTTAGACTTTATAAACAAAAAACAAATTCCAACTTGGCTTCTTTAAAAAAAATACAAAATTCCAACTCATTACTTCTCTGCAACAATCCTTTTTTACTTAGGCTGAGATCAAATCAAATAAAATTCAAAGAATGTGATTATATTGACTGAATAAAACGAGTGATAATGAAGCAAATTCAAAAGGAAAGTATAATAATAATAATACGTACATGTGATGTGATGCGTTGTGCACGCAAAGGCACGTTGACCATATCAAAGCCTTTTGATATTATGAATAGAAAAACAAAAGAGCATTCGACCTTTTGCTTTGGTTGGAATCTTTGATTTGAAGACCTAATCTCGATTTTCGAGCTCTACTATTGTTAATAAAATCAATTATTATTATGTAAGTGTAGGGATAATAATGAAATGTGTGATATAATATCTGACAGATTGCTGATGAGATGAGGCCAAATCCATTGGTGATAAGTTTCTTGACAAAGCTTTGTAAAGTAATTCCAACTTGGAAAATTATTCCAACTCAAGATATCATTGACATTGCCTTCAAACAACCTCACGTTAGGAAACAGGTATTTATTTCTACACAATTTCTTTTCTTTTAATTTGACCATAGTTATTTTCACATGTTTGTAAATGTATAAATAGTTTTACCACTCAAAACAAATTTAGGGAAAAGCTCAAGAACTACTATTAGACATTTGGAAGTACATAACAAAAAAGACCTACGTAGACCAATAATGATGAAAGACAATCATTGAAAGTTAGGATTGAAATGTATTTAATTGGACGAACTAAATCGGCTAATTTTTCATCCTTACCATTTAAAACTTTTGTTTTGCATTGGAAAATATAATAACCAAAAACTTAATCACAGCCATTTTCAATATTCACAAACAAAAGTTACAAAAAGGGATTTTGAAAATGCCAATAAATAAAACAACAATTTCTTTGTTGGCAGATTAGGGAAAATGCATATTGTTACAAGGGCCGGCCACGGTTGAGAACTGGCTATGAACTCTTGAGAATTACTTCATTACTTGAAAGCAAACTCCATGAGGTATTTCTTTTTCTTTATTTGTATTTGTTTGATTAATTTTCTTTCCTTTTCTTTTCCTTCTTTATATCTAGTTTTTGTTTGTTTCTCCTTTAGGTATCTCTACCTTTCCTCCTTCTCCACGGCGAAGACGACCGGGTTACCGACAAATTGGTTAGCAAACAACTCTACGACGATGCGGCAAGTGATGACAAGACGTTGAATATGTATCCAGGAATGTGGCATGGTTTGTTGTATGGCGAGACGCCAGAGAATATTGACATTGTGTTCTCAGATATCATCGGTTGGTTGGATAAAAGGAGTATCGTTGAAAATTTAAAATCAGAACTCGAGAGAAAGTACGAAAATGATGGTTTATTGGAAGCCAAGAAATAGCAAGAAAGCTTATTGATAACTTCAATCATATTTGTTGTCTTTGGTTTGGTTTGATTTGTTTGTGATATGTAAATAATTTATAAACATTGATTTTTATTAGAAGTATATTGGAACGTTCTCTTTAAGCCGATAG

mRNA sequence

CAGTAATTTCCCTTCTCTAGTTTTCTCTCGACGGCAATGGAGATTGAGAATGTAAGATATGAAGAGGAAGTAATAGTGAATGGACGTGGGTTAAAGTTGTTCACGTGCAATTGGGTTCCAAAGAATGAAGAACCAAAAGCTTTGATTTTTCTCTGCCATGGGTATGCCATGGAATGTAGCATCACAATGGACAGTAGTGCACGAAGATTAGCAAAAGAAGGATATGGAGTGTATGGAATTGATTATGAAGGACATGGAAAATCCAGTGGATTACAAGGGTATGTTTCAAGTTTTGATAACGTGGTGGACGATTGTTCTTCCTTTTTCACTTCAATATCAGAAAAGAAAGAAAATAGAGAGAAGAAAAGGTATTTGATGGGAGAGTCAATGGGAGGAGCAGTGGCTCTGATGATACACAGAAAACAGCCTGATTTTTGGGATGGTGCCATCTTGGTTGCTCCTATGTGTAAGATTGCTGATGAGATGAGGCCAAATCCATTGGTGATAAGTTTCTTGACAAAGCTTTGTAAAGTAATTCCAACTTGGAAAATTATTCCAACTCAAGATATCATTGACATTGCCTTCAAACAACCTCACGTTAGGAAACAGATTAGGGAAAATGCATATTGTTACAAGGGCCGGCCACGGTTGAGAACTGGCTATGAACTCTTGAGAATTACTTCATTACTTGAAAGCAAACTCCATGAGGTATCTCTACCTTTCCTCCTTCTCCACGGCGAAGACGACCGGGTTACCGACAAATTGGTTAGCAAACAACTCTACGACGATGCGGCAAGTGATGACAAGACGTTGAATATGTATCCAGGAATGTGGCATGGTTTGTTGTATGGCGAGACGCCAGAGAATATTGACATTGTGTTCTCAGATATCATCGGTTGGTTGGATAAAAGGAGTATCGTTGAAAATTTAAAATCAGAACTCGAGAGAAAGTACGAAAATGATGGTTTATTGGAAGCCAAGAAATAGCAAGAAAGCTTATTGATAACTTCAATCATATTTGTTGTCTTTGGTTTGGTTTGATTTGTTTGTGATATGTAAATAATTTATAAACATTGATTTTTATTAGAAGTATATTGGAACGTTCTCTTTAAGCCGATAG

Coding sequence (CDS)

ATGGAGATTGAGAATGTAAGATATGAAGAGGAAGTAATAGTGAATGGACGTGGGTTAAAGTTGTTCACGTGCAATTGGGTTCCAAAGAATGAAGAACCAAAAGCTTTGATTTTTCTCTGCCATGGGTATGCCATGGAATGTAGCATCACAATGGACAGTAGTGCACGAAGATTAGCAAAAGAAGGATATGGAGTGTATGGAATTGATTATGAAGGACATGGAAAATCCAGTGGATTACAAGGGTATGTTTCAAGTTTTGATAACGTGGTGGACGATTGTTCTTCCTTTTTCACTTCAATATCAGAAAAGAAAGAAAATAGAGAGAAGAAAAGGTATTTGATGGGAGAGTCAATGGGAGGAGCAGTGGCTCTGATGATACACAGAAAACAGCCTGATTTTTGGGATGGTGCCATCTTGGTTGCTCCTATGTGTAAGATTGCTGATGAGATGAGGCCAAATCCATTGGTGATAAGTTTCTTGACAAAGCTTTGTAAAGTAATTCCAACTTGGAAAATTATTCCAACTCAAGATATCATTGACATTGCCTTCAAACAACCTCACGTTAGGAAACAGATTAGGGAAAATGCATATTGTTACAAGGGCCGGCCACGGTTGAGAACTGGCTATGAACTCTTGAGAATTACTTCATTACTTGAAAGCAAACTCCATGAGGTATCTCTACCTTTCCTCCTTCTCCACGGCGAAGACGACCGGGTTACCGACAAATTGGTTAGCAAACAACTCTACGACGATGCGGCAAGTGATGACAAGACGTTGAATATGTATCCAGGAATGTGGCATGGTTTGTTGTATGGCGAGACGCCAGAGAATATTGACATTGTGTTCTCAGATATCATCGGTTGGTTGGATAAAAGGAGTATCGTTGAAAATTTAAAATCAGAACTCGAGAGAAAGTACGAAAATGATGGTTTATTGGAAGCCAAGAAATAG

Protein sequence

MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKSELERKYENDGLLEAKK*

Homology

[Close]

BLAST of CSPI05G09230 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.3e-43
Identity = 92/274 (33.58%), Postives = 146/274 (53.28%), Query Frame = 0


Query: 20  KLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEGYGVYGIDYEGHGKSSGL 79
           KLFT +++P + E K  +++ HGY  + S          +  GY V+  D  GHG+S G+
Sbjct: 50  KLFTQSFLPLDGEIKGTVYMSHGYGSDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGI 109

Query: 80  QGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAVALMIH-RKQPDFWDGAI 139
           + Y+   + V     +FF  +      ++   +L GESMGG V L+++ + +P+ W G +
Sbjct: 110 RCYMGDMEKVAATSLAFFKHVRCSDPYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLM 169

Query: 140 LVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIAFKQPHVRKQIRENAYC 199
             AP+  I ++M+P+   +     L  +  TW  +P   ++  A K P   K I  N   
Sbjct: 170 FSAPLFVIPEDMKPSKAHLFAYGLLFGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQR 229

Query: 200 YKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDKLVSKQLYDDAASDDKT 259
           Y G+PR+ T  ELLR T  ++    +V++P    HG  D VT    SK LY+ A+S DKT
Sbjct: 230 YTGKPRVGTMRELLRKTQYVQENFGKVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKT 289

Query: 260 LNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKR 293
           L +Y GM+H L+ GE  EN +IV  D+  W+D++
Sbjct: 290 LKIYEGMYHSLIQGEPDENAEIVLKDMREWIDEK 323

[Close]

BLAST of CSPI05G09230 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.7e-27
Identity = 90/293 (30.72%), Postives = 146/293 (49.83%), Query Frame = 0


Query: 4   ENVRYEE-EVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEG 63
           +NV Y++   +VN  G  LF   W P    PKALIF+ HG    C    D  A+ L +  
Sbjct: 12  QNVPYQDLPHLVNADGQYLFCRYWKPSG-TPKALIFVSHGAGEHCG-RYDELAQMLKRLD 71

Query: 64  YGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAV 123
             V+  D+ GHG+S G +  VS F   V D      ++  +K+  E   +L+G SMGGA+
Sbjct: 72  MLVFAHDHVGHGQSEGERMVVSDFQVFVRDLLQHVNTV--QKDYPEVPVFLLGHSMGGAI 131

Query: 124 ALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIA 183
           +++   ++P  + G IL++P+  +A+    + L +     L  V+P   +      + ++
Sbjct: 132 SILAAAERPTHFSGMILISPLI-LANPESASTLKVLAAKLLNFVLPNISLGRIDSSV-LS 191

Query: 184 FKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDK 243
             +  V     +   C+ G  ++  G +LL   S +E  +  ++LPFLLL G  DR+ D 
Sbjct: 192 RNKSEVDLYNSDPLICHAG-VKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDS 251

Query: 244 LVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIV 296
             +  L + + S DKTL MY G +H +L+ E PE  + V  +I  W+  R  V
Sbjct: 252 KGAYLLMESSPSQDKTLKMYEGAYH-VLHKELPEVTNSVLHEINTWVSHRIAV 296

[Close]

BLAST of CSPI05G09230 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 5.7e-26
Identity = 86/290 (29.66%), Postives = 141/290 (48.62%), Query Frame = 0


Query: 4   ENVRYEE-EVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEG 63
           +NV Y++   +VN  G  LF   W P    PKALIF+ HG    C    D  A  L    
Sbjct: 12  QNVPYQDLPHLVNADGQYLFCRYWKPSG-TPKALIFVSHGAGEHCG-RYDELAHMLKGLD 71

Query: 64  YGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAV 123
             V+  D+ GHG+S G +  VS F   V D      +I  +K+  +   +L+G SMGGA+
Sbjct: 72  MLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTI--QKDYPDVPIFLLGHSMGGAI 131

Query: 124 ALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIA 183
           ++++  ++P ++ G +L++P+     E      V++   KL   +     +   D   ++
Sbjct: 132 SILVAAERPTYFSGMVLISPLVLANPESASTLKVLA--AKLLNFVLPNMTLGRIDSSVLS 191

Query: 184 FKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDK 243
             +  V     +   C  G  ++  G +LL   + +E  +  ++LPFLLL G  DR+ D 
Sbjct: 192 RNKSEVDLYNSDPLVCRAGL-KVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDS 251

Query: 244 LVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKR 293
             +  L + + S DKTL MY G +H +L+ E PE  + V  ++  W+  R
Sbjct: 252 KGAYLLMESSRSQDKTLKMYEGAYH-VLHRELPEVTNSVLHEVNSWVSHR 293

[Close]

BLAST of CSPI05G09230 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 1.1e-24
Identity = 89/281 (31.67%), Postives = 141/281 (50.18%), Query Frame = 0


Query: 13  IVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEGYGVYGIDYEG 72
           +VN  G  LF   W P    PKALIF+ HG A E S   +  AR L      V+  D+ G
Sbjct: 22  LVNADGQYLFCRYWKPTG-TPKALIFVSHG-AGEHSGRYEELARMLMGLDLLVFAHDHVG 81

Query: 73  HGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAVALMIHRKQPD 132
           HG+S G +  VS F   V D      S+  +K+      +L+G SMGGA+A++   ++P 
Sbjct: 82  HGQSEGERMVVSDFHVFVRDVLQHVDSM--QKDYPGLPVFLLGHSMGGAIAILTAAERPG 141

Query: 133 FWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIAFKQPHVRKQI 192
            + G +L++P+  +A+        +     L  V+P   + P    + ++  +  V    
Sbjct: 142 HFAGMVLISPLV-LANPESATTFKVLAAKVLNLVLPNLSLGPIDSSV-LSRNKTEVDIYN 201

Query: 193 RENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDKLVSKQLYDDA 252
            +   C  G  ++  G +LL   S +E  L ++++PFLLL G  DR+ D   +  L + A
Sbjct: 202 SDPLICRAGL-KVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELA 261

Query: 253 ASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRS 294
            S DKTL +Y G +H +L+ E PE  + VF +I  W+ +R+
Sbjct: 262 KSQDKTLKIYEGAYH-VLHKELPEVTNSVFHEINMWVSQRT 294

[Close]

BLAST of CSPI05G09230 vs. ExPASy Swiss-Prot
Match: O07427 (Monoacylglycerol lipase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv0183 PE=1 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 1.4e-21
Identity = 77/274 (28.10%), Postives = 126/274 (45.99%), Query Frame = 0


Query: 26  WVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEGYGVYGIDYEGHGKSSG------- 85
           W P +  P+A++ L HG   E +   D  A+RL   G   Y +D+ GHG+S G       
Sbjct: 23  WTP-DTAPQAVVVLAHGLG-EHARRYDHVAQRLGAAGLVTYALDHRGHGRSGGKRVLVRD 82

Query: 86  LQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAVALMIHRKQPDFWDGAI 145
           +  Y + FD +V   +         +E    KR ++G SMGG +      ++PD +D  +
Sbjct: 83  ISEYTADFDTLVGIAT---------REYPGCKRIVLGHSMGGGIVFAYGVERPDNYDLMV 142

Query: 146 LVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIAFKQPHVRKQIRENAYC 205
           L AP     D + P   V++   KL  V+     +   D   I+ + P V +    +   
Sbjct: 143 LSAPAVAAQDLVSP---VVAVAAKLLGVVVPGLPVQELDFTAIS-RDPEVVQAYNTDPLV 202

Query: 206 YKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDKLVSKQLYDDAASDDKT 265
           + GR     G  LL++   +  +   ++ P L+LHG DDR+     S++L +   S D  
Sbjct: 203 HHGRVPAGIGRALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVECVGSADVQ 262

Query: 266 LNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKR 293
           L  YPG++H +     PE  + V  D++ WL +R
Sbjct: 263 LKEYPGLYHEVF--NEPER-NQVLDDVVAWLTER 278

[Close]

BLAST of CSPI05G09230 vs. ExPASy TrEMBL
Match: A0A0A0KS48 (Esterase/lipase/thioesterase family protein OS=Cucumis sativus OX=3659 GN=Csa_5G179220 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 4.9e-182
Identity = 314/316 (99.37%), Postives = 315/316 (99.68%), Query Frame = 0


Query: 1   MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK 60
           MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK
Sbjct: 1   MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK 60

Query: 61  EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGG 120
           EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGG
Sbjct: 61  EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGG 120

Query: 121 AVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIID 180
           AVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVIS LTKLCKVIPTWKIIPTQDIID
Sbjct: 121 AVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISLLTKLCKVIPTWKIIPTQDIID 180

Query: 181 IAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVT 240
           IAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLE+KLHEVSLPFLLLHGEDDRVT
Sbjct: 181 IAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLETKLHEVSLPFLLLHGEDDRVT 240

Query: 241 DKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKS 300
           DKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKS
Sbjct: 241 DKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKS 300

Query: 301 ELERKYENDGLLEAKK 317
           ELERKYENDGLLEAKK
Sbjct: 301 ELERKYENDGLLEAKK 316

[Close]

BLAST of CSPI05G09230 vs. ExPASy TrEMBL
Match: A0A5A7V5I5 (Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold321G00130 PE=4 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.9e-160
Identity = 280/316 (88.61%), Postives = 291/316 (92.09%), Query Frame = 0


Query: 1   MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK 60
           M+ ENVRYEEEVI NGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK
Sbjct: 1   MDTENVRYEEEVIENGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK 60

Query: 61  EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGG 120
           EGYGVYGIDYEGHGKSSGLQGYVSSFDNVV+DCSSFFTSIS              ESMGG
Sbjct: 61  EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVEDCSSFFTSIS--------------ESMGG 120

Query: 121 AVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIID 180
           AVALMIH KQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCK+IPTWKIIPTQD+ID
Sbjct: 121 AVALMIHIKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKIIPTWKIIPTQDVID 180

Query: 181 IAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVT 240
            AFK P VRKQIREN YCYKGRPRL+TGYELLR+T+LLE+KL+EVSLPFLLLHGEDDRVT
Sbjct: 181 FAFKLPQVRKQIRENPYCYKGRPRLKTGYELLRVTALLENKLNEVSLPFLLLHGEDDRVT 240

Query: 241 DKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKS 300
           DKLVSKQLYD+AASDDKTLNMY GMWHGLLYGETPEN+DIVFSDIIGWLDKRSI ENLKS
Sbjct: 241 DKLVSKQLYDEAASDDKTLNMYQGMWHGLLYGETPENVDIVFSDIIGWLDKRSIDENLKS 300

Query: 301 ELERKYENDGLLEAKK 317
           ELERK ENDGLLEA K
Sbjct: 301 ELERKKENDGLLEATK 302

[Close]

BLAST of CSPI05G09230 vs. ExPASy TrEMBL
Match: A0A6J1FPF0 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111447044 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 9.3e-157
Identity = 270/316 (85.44%), Postives = 294/316 (93.04%), Query Frame = 0


Query: 3   IENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEG 62
           +ENV YEEE IVN RGLKLFTC+WVPKN+EPKAL+FLCHGYAMECSITMDSSARRLA+EG
Sbjct: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60

Query: 63  YGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAV 122
           YGVYGIDYEGHGKSSGLQGYVSSFD VVDDCSSFFTSI+EK ENREKKRYLMGESMGGAV
Sbjct: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120

Query: 123 ALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIA 182
           AL++HRKQPD+WDGAILVAPMCKIA++MRPNPL ISFLTKLCK+IPTWKIIPTQDIIDIA
Sbjct: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180

Query: 183 FKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDK 242
           FK P +RKQIREN YCYKGRPRL+TG+ELLRI+S LE KL EVS+PFLLLHGEDDRVTD+
Sbjct: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240

Query: 243 LVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKSEL 302
           LVSKQLYD+A SDDKTL MYPGMWHGLLYGETPENIDIVFSDII WL+KR    N+KSEL
Sbjct: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR----NVKSEL 300

Query: 303 ERKYEND--GLLEAKK 317
           E+KYEND  G LE+K+
Sbjct: 301 EKKYENDHLGGLESKQ 312

[Close]

BLAST of CSPI05G09230 vs. ExPASy TrEMBL
Match: A0A6J1JZB9 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111489636 PE=4 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.7e-153
Identity = 264/316 (83.54%), Postives = 290/316 (91.77%), Query Frame = 0


Query: 3   IENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEG 62
           +ENV YEEE IVN RGLKLFTC+WVPK +EPKAL+FLCHGYAMECSITMDSSARRLA+EG
Sbjct: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKTDEPKALVFLCHGYAMECSITMDSSARRLAREG 60

Query: 63  YGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAV 122
           YGVYGIDYEGHGKS GLQGYVSSFD VVDDCSSFFTSI+EKKEN +KKRYLMGESMGGAV
Sbjct: 61  YGVYGIDYEGHGKSFGLQGYVSSFDKVVDDCSSFFTSIAEKKENEKKKRYLMGESMGGAV 120

Query: 123 ALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIA 182
           AL++HRKQPD+WDGAILVAPMCKIA++MRPNPL ISFL+KLCK+IPTWKIIPTQDIIDIA
Sbjct: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLSKLCKLIPTWKIIPTQDIIDIA 180

Query: 183 FKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDK 242
           FK P +RKQ+REN YCYKGRPRL+TGYELLRI+S LE KL EVS+PFLLLHGEDDRVTDK
Sbjct: 181 FKVPEIRKQVRENRYCYKGRPRLKTGYELLRISSELEHKLDEVSVPFLLLHGEDDRVTDK 240

Query: 243 LVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKSEL 302
           LVSKQLYD+A SDDKTL MY GMWHGLLYGETPENIDIVFSDII WL+KR    N+KSE 
Sbjct: 241 LVSKQLYDEAVSDDKTLKMYSGMWHGLLYGETPENIDIVFSDIIDWLEKR----NVKSEF 300

Query: 303 ERKYEND--GLLEAKK 317
           E+K+END  G LE+K+
Sbjct: 301 EKKHENDHHGGLESKQ 312

[Close]

BLAST of CSPI05G09230 vs. ExPASy TrEMBL
Match: A0A6J1CCN8 (caffeoylshikimate esterase-like OS=Momordica charantia OX=3673 GN=LOC111010007 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.3e-147
Identity = 251/317 (79.18%), Postives = 289/317 (91.17%), Query Frame = 0


Query: 1   MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK 60
           +E ENV+YEEE + + RGLKLFTC+W+P  EEPKALIF+CHGYAMECSITM+S+ARRLAK
Sbjct: 3   IEEENVKYEEEFVESARGLKLFTCSWIPVKEEPKALIFICHGYAMECSITMNSTARRLAK 62

Query: 61  EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGG 120
           EGYG+YG+DYEGHGKSSGLQG+VSSFDNVV+DCS+FFTSI+EKKEN +KKRYL+GESMGG
Sbjct: 63  EGYGIYGMDYEGHGKSSGLQGFVSSFDNVVEDCSTFFTSITEKKENGKKKRYLLGESMGG 122

Query: 121 AVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIID 180
           AVAL+IHRKQPD+WDGAILVAPMCKIA +MRPNP++IS LTKL K+IPTWK+IPT+DIID
Sbjct: 123 AVALLIHRKQPDYWDGAILVAPMCKIAKDMRPNPMMISMLTKLSKLIPTWKLIPTEDIID 182

Query: 181 IAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVT 240
           IAFK P VRKQIREN+YCYKGRPRL+TGYELLR +S LE  L EV LPFL+LHGEDDRVT
Sbjct: 183 IAFKVPEVRKQIRENSYCYKGRPRLKTGYELLRTSSELERNLKEVCLPFLVLHGEDDRVT 242

Query: 241 DKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVE-NLK 300
           DKLVSKQLYD+A+SDDKTL MYPGMWHGLLYGETPENIDIVF+DI  WL++RS+   + +
Sbjct: 243 DKLVSKQLYDEASSDDKTLKMYPGMWHGLLYGETPENIDIVFADITSWLEERSVANGDSR 302

Query: 301 SELERKYENDGLLEAKK 317
           SE E K ENDGLLEAK+
Sbjct: 303 SEHEIKRENDGLLEAKQ 319

[Close]

BLAST of CSPI05G09230 vs. NCBI nr
Match: XP_004147709.1 (caffeoylshikimate esterase [Cucumis sativus] >KGN50506.1 hypothetical protein Csa_000574 [Cucumis sativus])

HSP 1 Score: 646.7 bits (1667), Expect = 1.0e-181
Identity = 314/316 (99.37%), Postives = 315/316 (99.68%), Query Frame = 0


Query: 1   MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK 60
           MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK
Sbjct: 1   MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK 60

Query: 61  EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGG 120
           EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGG
Sbjct: 61  EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGG 120

Query: 121 AVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIID 180
           AVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVIS LTKLCKVIPTWKIIPTQDIID
Sbjct: 121 AVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISLLTKLCKVIPTWKIIPTQDIID 180

Query: 181 IAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVT 240
           IAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLE+KLHEVSLPFLLLHGEDDRVT
Sbjct: 181 IAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLETKLHEVSLPFLLLHGEDDRVT 240

Query: 241 DKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKS 300
           DKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKS
Sbjct: 241 DKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKS 300

Query: 301 ELERKYENDGLLEAKK 317
           ELERKYENDGLLEAKK
Sbjct: 301 ELERKYENDGLLEAKK 316

[Close]

BLAST of CSPI05G09230 vs. NCBI nr
Match: XP_038891440.1 (caffeoylshikimate esterase-like [Benincasa hispida])

HSP 1 Score: 584.3 bits (1505), Expect = 6.2e-163
Identity = 280/317 (88.33%), Postives = 301/317 (94.95%), Query Frame = 0


Query: 2   EIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKE 61
           +I+NVRYEEEVIVN  GLKLFTCNW+PKNEE KALIF+CHGYAMECSITM+S+ARRLAKE
Sbjct: 4   QIDNVRYEEEVIVNAHGLKLFTCNWIPKNEESKALIFICHGYAMECSITMNSTARRLAKE 63

Query: 62  GYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGA 121
           GYGVYGIDYEGHGKS GLQGYVSSFDNVV+DCSSFFTSISEKKENREKKRYLMGESMGGA
Sbjct: 64  GYGVYGIDYEGHGKSGGLQGYVSSFDNVVEDCSSFFTSISEKKENREKKRYLMGESMGGA 123

Query: 122 VALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDI 181
           VAL+IHRKQPDFWDGAILVAPMCKIAD+MRP+PLVIS LTKLCK+IPTWKIIPTQD+IDI
Sbjct: 124 VALLIHRKQPDFWDGAILVAPMCKIADDMRPHPLVISVLTKLCKIIPTWKIIPTQDVIDI 183

Query: 182 AFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTD 241
           AFK P +RKQIREN YCYKGRPRL+TGYELLRI+S LE KL+EVSLPFLLLHGEDDRVTD
Sbjct: 184 AFKVPEIRKQIRENPYCYKGRPRLKTGYELLRISSDLEHKLNEVSLPFLLLHGEDDRVTD 243

Query: 242 KLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIV--ENLK 301
           KLVSKQLYD+A SDDKTL MYPGMWHGLLYGETPEN+DIVFSDII WL+KRSIV  EN K
Sbjct: 244 KLVSKQLYDEALSDDKTLKMYPGMWHGLLYGETPENMDIVFSDIISWLEKRSIVAYENSK 303

Query: 302 SELERKYENDGLLEAKK 317
           SELE+KYENDGLLEAK+
Sbjct: 304 SELEKKYENDGLLEAKQ 320

[Close]

BLAST of CSPI05G09230 vs. NCBI nr
Match: KAA0062217.1 (caffeoylshikimate esterase [Cucumis melo var. makuwa])

HSP 1 Score: 573.2 bits (1476), Expect = 1.4e-159
Identity = 280/316 (88.61%), Postives = 291/316 (92.09%), Query Frame = 0


Query: 1   MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK 60
           M+ ENVRYEEEVI NGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK
Sbjct: 1   MDTENVRYEEEVIENGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK 60

Query: 61  EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGG 120
           EGYGVYGIDYEGHGKSSGLQGYVSSFDNVV+DCSSFFTSIS              ESMGG
Sbjct: 61  EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVEDCSSFFTSIS--------------ESMGG 120

Query: 121 AVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIID 180
           AVALMIH KQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCK+IPTWKIIPTQD+ID
Sbjct: 121 AVALMIHIKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKIIPTWKIIPTQDVID 180

Query: 181 IAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVT 240
            AFK P VRKQIREN YCYKGRPRL+TGYELLR+T+LLE+KL+EVSLPFLLLHGEDDRVT
Sbjct: 181 FAFKLPQVRKQIRENPYCYKGRPRLKTGYELLRVTALLENKLNEVSLPFLLLHGEDDRVT 240

Query: 241 DKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKS 300
           DKLVSKQLYD+AASDDKTLNMY GMWHGLLYGETPEN+DIVFSDIIGWLDKRSI ENLKS
Sbjct: 241 DKLVSKQLYDEAASDDKTLNMYQGMWHGLLYGETPENVDIVFSDIIGWLDKRSIDENLKS 300

Query: 301 ELERKYENDGLLEAKK 317
           ELERK ENDGLLEA K
Sbjct: 301 ELERKKENDGLLEATK 302

[Close]

BLAST of CSPI05G09230 vs. NCBI nr
Match: XP_023547317.1 (caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 564.7 bits (1454), Expect = 5.1e-157
Identity = 271/316 (85.76%), Postives = 295/316 (93.35%), Query Frame = 0


Query: 3   IENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEG 62
           +ENV YEEE IVN RGLKLFTC+WVPKNEEPKAL+FLCHGYAMECSITMDSSARRLAKEG
Sbjct: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNEEPKALVFLCHGYAMECSITMDSSARRLAKEG 60

Query: 63  YGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAV 122
           YGVYG+DYEGHGKSSGLQGYVSSF+NVVDDCSSFFTSI+EKKENREKKRYLMGESMGGAV
Sbjct: 61  YGVYGVDYEGHGKSSGLQGYVSSFNNVVDDCSSFFTSIAEKKENREKKRYLMGESMGGAV 120

Query: 123 ALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIA 182
           A+++HRKQP++WDGAILVAPMCKIA++MRPN L ISFLTKLCK+IPTWKIIPTQDIIDIA
Sbjct: 121 AVLVHRKQPEYWDGAILVAPMCKIAEDMRPNALTISFLTKLCKIIPTWKIIPTQDIIDIA 180

Query: 183 FKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDK 242
           FK P +RKQIREN YCYKGRPRL+TGYELLRI+S LE KL EVS+PFLLLHGEDDRVTDK
Sbjct: 181 FKVPEIRKQIRENRYCYKGRPRLKTGYELLRISSELEHKLDEVSVPFLLLHGEDDRVTDK 240

Query: 243 LVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKSEL 302
           LVSKQLYD+A SDDKTL MYPGMWHGLLYGETPENIDIVFSDII WL+KR    N+KSEL
Sbjct: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR----NVKSEL 300

Query: 303 ERKYEND--GLLEAKK 317
           E+KYEND  G LE+K+
Sbjct: 301 EKKYENDRRGGLESKQ 312

[Close]

BLAST of CSPI05G09230 vs. NCBI nr
Match: XP_022941777.1 (caffeoylshikimate esterase-like [Cucurbita moschata] >XP_022941778.1 caffeoylshikimate esterase-like [Cucurbita moschata] >XP_022941779.1 caffeoylshikimate esterase-like [Cucurbita moschata])

HSP 1 Score: 562.8 bits (1449), Expect = 1.9e-156
Identity = 270/316 (85.44%), Postives = 294/316 (93.04%), Query Frame = 0


Query: 3   IENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEG 62
           +ENV YEEE IVN RGLKLFTC+WVPKN+EPKAL+FLCHGYAMECSITMDSSARRLA+EG
Sbjct: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60

Query: 63  YGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAV 122
           YGVYGIDYEGHGKSSGLQGYVSSFD VVDDCSSFFTSI+EK ENREKKRYLMGESMGGAV
Sbjct: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120

Query: 123 ALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIA 182
           AL++HRKQPD+WDGAILVAPMCKIA++MRPNPL ISFLTKLCK+IPTWKIIPTQDIIDIA
Sbjct: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180

Query: 183 FKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDK 242
           FK P +RKQIREN YCYKGRPRL+TG+ELLRI+S LE KL EVS+PFLLLHGEDDRVTD+
Sbjct: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240

Query: 243 LVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKSEL 302
           LVSKQLYD+A SDDKTL MYPGMWHGLLYGETPENIDIVFSDII WL+KR    N+KSEL
Sbjct: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR----NVKSEL 300

Query: 303 ERKYEND--GLLEAKK 317
           E+KYEND  G LE+K+
Sbjct: 301 EKKYENDHLGGLESKQ 312

[Close]

BLAST of CSPI05G09230 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 468.0 bits (1203), Expect = 6.0e-132
Identity = 214/310 (69.03%), Postives = 262/310 (84.52%), Query Frame = 0


Query: 2   EIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKE 61
           E EN++YEE  I N RG+KLFTC WVP  +EPKAL+F+CHGYAMECSITM+S+ARRL K 
Sbjct: 4   ETENIKYEESFIKNTRGMKLFTCKWVPAKQEPKALVFICHGYAMECSITMNSTARRLVKA 63

Query: 62  GYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGA 121
           G+ VYGIDYEGHGKS GL  YV +FD++VDD S+ +TSI EK+EN+ K R+L+GESMGGA
Sbjct: 64  GFAVYGIDYEGHGKSDGLSAYVPNFDHLVDDVSTHYTSICEKEENKGKMRFLLGESMGGA 123

Query: 122 VALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDI 181
           V L++HRK+P FWDGA+LVAPMCKIA+EM+P+PLVIS L KL  VIP+WKIIP QDII+ 
Sbjct: 124 VLLLLHRKKPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSWKIIPGQDIIET 183

Query: 182 AFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTD 241
           AFKQP +RKQ+REN YCYKGRPRL+T YELLR+++ LE +L+EVSLPF++LHGEDD+VTD
Sbjct: 184 AFKQPEIRKQVRENPYCYKGRPRLKTAYELLRVSTDLEKRLNEVSLPFIVLHGEDDKVTD 243

Query: 242 KLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKSE 301
           K VS+QLY+ A+S DKT  +YPGMWHGLLYGETPENI+ VF+DIIGWLDK+   E+   E
Sbjct: 244 KAVSRQLYEVASSSDKTFKLYPGMWHGLLYGETPENIETVFADIIGWLDKKVADESGGFE 303

Query: 302 LERKYENDGL 312
            E K +NDG+
Sbjct: 304 SELKRKNDGI 313

[Close]

BLAST of CSPI05G09230 vs. TAIR 10
Match: AT2G39410.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 445.7 bits (1145), Expect = 3.2e-125
Identity = 204/311 (65.59%), Postives = 255/311 (81.99%), Query Frame = 0


Query: 1   MEIENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAK 60
           +E E+++YEE  I N RG KLFTC W+P N EP+AL+FLCHGY MECSITM+S+ARRL K
Sbjct: 3   IETEDIKYEESFIKNTRGFKLFTCRWLPTNREPRALVFLCHGYGMECSITMNSTARRLVK 62

Query: 61  EGYGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGG 120
            G+ VYG+DYEGHGKS GL  Y+S+FD +VDD S+ +T+I E++EN+ K R+++GESMGG
Sbjct: 63  AGFAVYGMDYEGHGKSDGLSAYISNFDRLVDDVSTHYTAICEREENKWKMRFMLGESMGG 122

Query: 121 AVALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIID 180
           AV L++ RK PDFWDGAILVAPMCKIA+EM+P+P VIS LTKL  +IP WKIIP+QDII+
Sbjct: 123 AVVLLLGRKNPDFWDGAILVAPMCKIAEEMKPSPFVISILTKLISIIPKWKIIPSQDIIE 182

Query: 181 IAFKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVT 240
           I++K+P +RKQ+REN  C KGRPRL+T YELLRI++ LE +L EVSLPFL+LHG+DD+VT
Sbjct: 183 ISYKEPEIRKQVRENPLCSKGRPRLKTAYELLRISNDLEKRLQEVSLPFLVLHGDDDKVT 242

Query: 241 DKLVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKS 300
           DK VS++LY  A S DKTL +YPGMWHGLL GETPENI+IVF+D+I WL+KRS   N + 
Sbjct: 243 DKAVSQELYKVALSADKTLKLYPGMWHGLLTGETPENIEIVFADVISWLEKRSDYGNDRF 302

Query: 301 ELERKYENDGL 312
           E E K  ND L
Sbjct: 303 ESELKQRNDRL 313

[Close]

BLAST of CSPI05G09230 vs. TAIR 10
Match: AT2G39400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 431.0 bits (1107), Expect = 8.1e-121
Identity = 194/310 (62.58%), Postives = 250/310 (80.65%), Query Frame = 0


Query: 6   VRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEGYGV 65
           V YEE+ ++N RG+KLFTC W P  +EPKAL+FLCHGYAME SITM+S+A RLA  G+ V
Sbjct: 2   VMYEEDFVLNSRGMKLFTCVWKPVKQEPKALLFLCHGYAMESSITMNSAATRLANAGFAV 61

Query: 66  YGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAVALM 125
           YG+DYEGHGKS GL GY+S+FD++VDD S+ +++I E++EN+ K R+L+GESMGGAV L+
Sbjct: 62  YGMDYEGHGKSEGLNGYISNFDDLVDDVSNHYSTICEREENKGKMRFLLGESMGGAVVLL 121

Query: 126 IHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIAFKQ 185
           + RK+PDFWDGA+LVAPMCK+ADE++P+P+VIS L KL K IPTWKI+P  DIIDIA K+
Sbjct: 122 LARKKPDFWDGAVLVAPMCKLADEIKPHPVVISILIKLAKFIPTWKIVPGNDIIDIAIKE 181

Query: 186 PHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDKLVS 245
           PH+R Q+REN YCYKGRPRL T Y+LL ++  LE  LH+VS+PF++LHGEDD+VTDK +S
Sbjct: 182 PHIRNQVRENKYCYKGRPRLNTAYQLLLVSLDLEKNLHQVSIPFIVLHGEDDKVTDKSIS 241

Query: 246 KQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKSELERK 305
           K LY+ A+S DKT  +YP MWH LLYGET EN +IVF DII WL+ R+   N   E + K
Sbjct: 242 KMLYEVASSSDKTFKLYPKMWHALLYGETNENSEIVFGDIINWLEDRATDSNGGLESQLK 301

Query: 306 YENDGLLEAK 316
           +++DG L+ K
Sbjct: 302 HKHDGFLKHK 311

[Close]

BLAST of CSPI05G09230 vs. TAIR 10
Match: AT3G55180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 425.2 bits (1092), Expect = 4.5e-119
Identity = 193/306 (63.07%), Postives = 251/306 (82.03%), Query Frame = 0


Query: 6   VRYEEEVIVNGRGLKLFTCNW-VPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEGYG 65
           V Y+E+ + N RG++LFTC+W   + +EPKALIFLCHGYAME SITM S+A RLA  G+ 
Sbjct: 2   VMYKEDYVSNSRGIQLFTCSWKQEEQQEPKALIFLCHGYAMESSITMSSTAVRLANAGFS 61

Query: 66  VYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAVAL 125
           VYG+DYEGHGKS GL GYV  FD++V D SS ++SI E +EN+ K R+LMGESMGGAV L
Sbjct: 62  VYGMDYEGHGKSGGLNGYVKKFDDLVQDVSSHYSSICELEENKGKMRFLMGESMGGAVVL 121

Query: 126 MIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWKIIPTQDIIDIAFK 185
           ++ RK+P+FWDGA+LVAPMCK+A++++P+P+VISFLTKL + IPTWKI+P+ DIID+AFK
Sbjct: 122 LLERKKPNFWDGAVLVAPMCKLAEDIKPHPMVISFLTKLTRFIPTWKIVPSNDIIDVAFK 181

Query: 186 QPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDKLV 245
           + H+RKQ+R+N YCYKGRPRL+T ++LL ++  LE  L +VS+PF++LHGEDD+VTDK V
Sbjct: 182 ETHIRKQVRDNEYCYKGRPRLKTAHQLLMVSLDLEKNLDQVSMPFIVLHGEDDKVTDKNV 241

Query: 246 SKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKSELER 305
           SK LY+ A+S DKT  +YP MWHGLLYGE+PEN++IVFSDII WL +R+ V N K E E 
Sbjct: 242 SKLLYEVASSSDKTFKLYPNMWHGLLYGESPENLEIVFSDIISWLKERASVTNQKLETEL 301

Query: 306 KYENDG 311
           K+ +DG
Sbjct: 302 KHVDDG 307

[Close]

BLAST of CSPI05G09230 vs. TAIR 10
Match: AT3G55190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 358.2 bits (918), Expect = 6.7e-99
Identity = 166/296 (56.08%), Postives = 222/296 (75.00%), Query Frame = 0


Query: 6   VRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEGYGV 65
           V Y EE I N RG++L TC W P N+EP+ALIF CHGYA++CS T    A + AKEG+ V
Sbjct: 8   VGYSEEFIENSRGMQLLTCKWFPVNQEPRALIFFCHGYAIDCSTTFKDIAPKFAKEGFAV 67

Query: 66  YGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAVALM 125
           +GI+YEGHG+SSGL  Y+ +FD ++DD SS F+ ISE  +N +KKR+LMGESMGGAV L+
Sbjct: 68  HGIEYEGHGRSSGLSVYIDNFDLLIDDVSSHFSKISEMGDNTKKKRFLMGESMGGAVVLL 127

Query: 126 IHRKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKVIPTWK-IIPTQDIIDIAFK 185
           +HRK+P+FWDG IL+APMCKIA+EM+P+ +VIS +  +  +IP+WK II   DI++ A K
Sbjct: 128 LHRKKPEFWDGGILIAPMCKIAEEMKPSRMVISMINMVTNLIPSWKSIIHGPDILNSAIK 187

Query: 186 QPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLESKLHEVSLPFLLLHGEDDRVTDKLV 245
            P  R +IR N  CY G PR++T  EL RI+  LE++L+EV++PF++LHGEDD+VTDK  
Sbjct: 188 LPEKRHEIRTNPNCYNGWPRMKTMSELFRISLDLENRLNEVTMPFIVLHGEDDKVTDKGG 247

Query: 246 SKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKS 301
           SK LY+ A S+DKTL +YP MWH LL+GE PEN +IVF+DI+ W+  R     +K+
Sbjct: 248 SKLLYEVALSNDKTLKLYPEMWHSLLFGEPPENSEIVFNDIVQWMQTRITTLQVKA 303

The following BLAST results are available for this feature:

BLAST of CSPI05G09230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C942	2.3e-43	33.58	Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1	[more]
Q8R431	8.7e-27	30.72	Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1	[more]
O35678	5.7e-26	29.66	Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1	[more]
Q99685	1.1e-24	31.67	Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2	[more]
O07427	1.4e-21	28.10	Monoacylglycerol lipase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv...	[more]

BLAST of CSPI05G09230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KS48	4.9e-182	99.37	Esterase/lipase/thioesterase family protein OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A5A7V5I5	6.9e-160	88.61	Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A6J1FPF0	9.3e-157	85.44	caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111447044 PE...	[more]
A0A6J1JZB9	3.7e-153	83.54	caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111489636 PE=4...	[more]
A0A6J1CCN8	1.3e-147	79.18	caffeoylshikimate esterase-like OS=Momordica charantia OX=3673 GN=LOC111010007 P...	[more]

BLAST of CSPI05G09230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147709.1	1.0e-181	99.37	caffeoylshikimate esterase [Cucumis sativus] >KGN50506.1 hypothetical protein Cs...	[more]
XP_038891440.1	6.2e-163	88.33	caffeoylshikimate esterase-like [Benincasa hispida]	[more]
KAA0062217.1	1.4e-159	88.61	caffeoylshikimate esterase [Cucumis melo var. makuwa]	[more]
XP_023547317.1	5.1e-157	85.76	caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022941777.1	1.9e-156	85.44	caffeoylshikimate esterase-like [Cucurbita moschata] >XP_022941778.1 caffeoylshi...	[more]

BLAST of CSPI05G09230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39420.1	6.0e-132	69.03	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39410.2	3.2e-125	65.59	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39400.1	8.1e-121	62.58	alpha/beta-Hydrolases superfamily protein	[more]
AT3G55180.1	4.5e-119	63.07	alpha/beta-Hydrolases superfamily protein	[more]
AT3G55190.1	6.7e-99	56.08	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 62..77 score: 36.21 coord: 113..126 score: 49.51 coord: 229..243 score: 33.1
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 3..295 e-value: 1.2E-59 score: 204.0
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 4..292
IPR022742	Serine aminopeptidase, S33	PFAM	PF12146	Hydrolase_4	coord: 32..270 e-value: 9.8E-66 score: 221.4
None	No IPR available	PANTHER	PTHR11614	PHOSPHOLIPASE-RELATED	coord: 8..306
None	No IPR available	PANTHER	PTHR11614:SF176	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 8..306

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G09230.1	CSPI05G09230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016298	lipase activity