CSPI05G08400 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI05G08400
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
Location	Chr5: 7111979 .. 7112614 (+)
RNA-Seq Expression	CSPI05G08400
Synteny	CSPI05G08400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGATACCTTCCTTTTCCATTCTTCTTCCATCTCTTCTTCTTCTTCTTCTACCTTCAGTTTTCATTATTCTCAAACACATCAAATCTCCATCTTCAAAAAAACTCCCACTTCCTCCTCGTCCAAATCCATGGCCAATCTTAGGAAGTCTCTTTCACATTGGTCAAAATGCTCACATTTCCATCACCCAATTTGCCAATATCTATGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGTCTCATCGTCGCTTCTTCTCGGGCTGCCACCACTGCAGTTCTCAGGACGCAAGACTGCCTTCTCTCTGCTCGATACATCTTTCAAATGACCCCTGATAGAGCTCTCCACGATCAGCACTCTCTTGTTTTCTCCCCTGAATATGGAGACCAATGGAAAAATTTGAGGTCAATTTGTAAAGTGAACTTGTTTACGGCTAAAGAAATTGAATCACAGACTACTTTGAGAGAGAAGAAGATGAAGGAGTTAGTGGAATTTCTGGAATCAAAACAAGGATCTGTTGTGGAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAAATCTCTTACTAACTTGATACAAAATCAAGTTAGAAAGCCAACAAACTTATTTCCAATTAAACTCACTTATATCTGA

mRNA sequence

ATGTTGATACCTTCCTTTTCCATTCTTCTTCCATCTCTTCTTCTTCTTCTTCTACCTTCAGTTTTCATTATTCTCAAACACATCAAATCTCCATCTTCAAAAAAACTCCCACTTCCTCCTCGTCCAAATCCATGGCCAATCTTAGGAAGTCTCTTTCACATTGGTCAAAATGCTCACATTTCCATCACCCAATTTGCCAATATCTATGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGTCTCATCGTCGCTTCTTCTCGGGCTGCCACCACTGCAGTTCTCAGGACGCAAGACTGCCTTCTCTCTGCTCGATACATCTTTCAAATGACCCCTGATAGAGCTCTCCACGATCAGCACTCTCTTGTTTTCTCCCCTGAATATGGAGACCAATGGAAAAATTTGAGGTCAATTTGTAAAGTGAACTTGTTTACGGCTAAAGAAATTGAATCACAGACTACTTTGAGAGAGAAGAAGATGAAGGAGTTAGTGGAATTTCTGGAATCAAAACAAGGATCTGTTGTGGAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAAATCTCTTACTAACTTGATACAAAATCAAGTTAGAAAGCCAACAAACTTATTTCCAATTAAACTCACTTATATCTGA

Coding sequence (CDS)

ATGTTGATACCTTCCTTTTCCATTCTTCTTCCATCTCTTCTTCTTCTTCTTCTACCTTCAGTTTTCATTATTCTCAAACACATCAAATCTCCATCTTCAAAAAAACTCCCACTTCCTCCTCGTCCAAATCCATGGCCAATCTTAGGAAGTCTCTTTCACATTGGTCAAAATGCTCACATTTCCATCACCCAATTTGCCAATATCTATGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGTCTCATCGTCGCTTCTTCTCGGGCTGCCACCACTGCAGTTCTCAGGACGCAAGACTGCCTTCTCTCTGCTCGATACATCTTTCAAATGACCCCTGATAGAGCTCTCCACGATCAGCACTCTCTTGTTTTCTCCCCTGAATATGGAGACCAATGGAAAAATTTGAGGTCAATTTGTAAAGTGAACTTGTTTACGGCTAAAGAAATTGAATCACAGACTACTTTGAGAGAGAAGAAGATGAAGGAGTTAGTGGAATTTCTGGAATCAAAACAAGGATCTGTTGTGGAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAAATCTCTTACTAACTTGATACAAAATCAAGTTAGAAAGCCAACAAACTTATTTCCAATTAAACTCACTTATATCTGA

Protein sequence

MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQHSLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDFVFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI*

Homology

[Close]

BLAST of CSPI05G08400 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 3.0e-31
Identity = 68/159 (42.77%), Postives = 105/159 (66.04%), Query Frame = 0


Query: 38  LPPRPNPWPILGSLFHIGQNAHISITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRT 97
           LPP P PWPI+G+L  +G+  H    + A  YG + +LK+G + ++VAS+ +A + +L+T
Sbjct: 28  LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKT 87

Query: 98  QDCLLSARYIFQMTPDRALHDQHSLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLRE 157
            D +LSARY+FQ    +  H ++S+V+S +  + WKNLR +C+  LFT K IESQ  +RE
Sbjct: 88  HDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVRE 147

Query: 158 KKMKELVEFLESKQGSVVEIKDFVFTSVFKSLTNLIQNQ 197
           KK +E+VE+L  KQG  V+I + +F ++     NLI +Q
Sbjct: 148 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 184

[Close]

BLAST of CSPI05G08400 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 9.6e-30
Identity = 73/189 (38.62%), Postives = 119/189 (62.96%), Query Frame = 0


Query: 8   ILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFAN 67
           ++  +L+ +++ S+  +L      SS    LPP P PWPI+G+L  +G+  H    + A 
Sbjct: 3   VVTVALIAVIISSILYLL----FGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQ 62

Query: 68  IYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQHSLVFSPE 127
            YG + +LK+G + ++VAS+ +A + +L+T D +LSARY+FQ    +  H ++S+V+S +
Sbjct: 63  TYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-D 122

Query: 128 YGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDFVFTSVFK 187
             + WKNLR +C+  LFT K IESQ  +REKK +E+VE+L  KQG  V+I + +F ++  
Sbjct: 123 CTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVN 182

Query: 188 SLTNLIQNQ 197
              NLI +Q
Sbjct: 183 IFGNLIFSQ 185

[Close]

BLAST of CSPI05G08400 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.8e-29
Identity = 73/185 (39.46%), Postives = 112/185 (60.54%), Query Frame = 0


Query: 12  SLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFANIYGP 71
           SL+ +++ +   ++    SP      LPP P PWPI+G+L  +G+  H    Q A  YG 
Sbjct: 1   SLVAVVITTFLYLIFRDSSPKG----LPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGD 60

Query: 72  LISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQHSLVFSPEYGDQ 131
           L SLKLG++ ++VAS+  A + +L+T D +LS RY+FQ    +  H ++S+V+S E  + 
Sbjct: 61  LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNET 120

Query: 132 WKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDFVFTSVFKSLTN 191
           WK LR +C+  LFT K IESQ  +RE K  E+VE+L+   G+ V+I + VF ++     N
Sbjct: 121 WKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 179

Query: 192 LIQNQ 197
           LI +Q
Sbjct: 181 LIFSQ 179

[Close]

BLAST of CSPI05G08400 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.2e-29
Identity = 83/193 (43.01%), Postives = 116/193 (60.10%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           M + S +I+  S LL L    F IL+      S+   LPP P P PI+G+L  +G   H 
Sbjct: 1   MEVLSIAIVSFSFLLFL----FFILR-----DSRPKNLPPGPRPSPIVGNLLQLGDKPHA 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
              + A  YG L SLKLG+Q ++VASS AA   +L+T D +LS RY+FQ    +  H ++
Sbjct: 61  EFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVEN 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           S+V+S E  D WK LR +C+  LFT K IESQ+ +RE K +E+V+FL  K+G VV+I + 
Sbjct: 121 SIVWS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEV 180

Query: 181 VFTSVFKSLTNLI 194
           VF ++     NLI
Sbjct: 181 VFGTLVNIFGNLI 182

[Close]

BLAST of CSPI05G08400 vs. ExPASy Swiss-Prot
Match: I3V6B1 ((S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum OX=3469 GN=CYP80BX PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.4e-25
Identity = 68/189 (35.98%), Postives = 110/189 (58.20%), Query Frame = 0


Query: 8   ILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFAN 67
           I+  +L+ ++  +   ++    SP+     LPP P PWPI+G+L  +G+  H    Q A 
Sbjct: 3   IVTVALIAIVFTTFLYLIVRESSPNG----LPPGPKPWPIVGNLLQLGEKPHSQFAQLAE 62

Query: 68  IYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQHSLVFSPE 127
            YG L +LKLG Q ++VAS+  A + VL+  D  L  RY+FQ    +  H ++S+V++ E
Sbjct: 63  TYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKN-HVENSIVWN-E 122

Query: 128 YGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDFVFTSVFK 187
             + WK LR +C+  LFT K IE+Q  +RE K  E+V++L+  +G  V+I + +F ++  
Sbjct: 123 CNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVN 182

Query: 188 SLTNLIQNQ 197
              NLI +Q
Sbjct: 183 MFGNLIFSQ 185

[Close]

BLAST of CSPI05G08400 vs. ExPASy TrEMBL
Match: A0A0A0KL66 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173470 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.0e-103
Identity = 200/211 (94.79%), Postives = 205/211 (97.16%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPP P+PWPI G+L  +GQNAH+
Sbjct: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPGPSPWPIFGNLLQMGQNAHV 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFAN+YGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANVYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFS EYGDQWKNLRSICKVNLFTAKEIESQ TLREKKMKELVEFLESKQGSVVEIKDF
Sbjct: 121 SLVFSHEYGDQWKNLRSICKVNLFTAKEIESQDTLREKKMKELVEFLESKQGSVVEIKDF 180

Query: 181 VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI 212
           VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI
Sbjct: 181 VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI 211

[Close]

BLAST of CSPI05G08400 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 8.6e-82
Identity = 170/193 (88.08%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLL   +FIILKHIKSPSS KLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 1   MLIPSFSILLPSLLLLL--PIFIILKHIKSPSS-KLPLPPGPNPWPILGNLLQIGQNAHI 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLISLKLGAQRLIVASS AA TAVL+TQD LLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFSPE GDQWKNLRS+CKVNLFTAK IESQ  LREKKMKELV+FLESKQGSVVE+KDF
Sbjct: 121 SLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDF 180

Query: 181 VFTSVFKSLTNLI 194
           VFTSVF +L+NLI
Sbjct: 181 VFTSVFNTLSNLI 190

[Close]

BLAST of CSPI05G08400 vs. ExPASy TrEMBL
Match: A0A5D3BKL7 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00360 PE=4 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 8.6e-82
Identity = 170/193 (88.08%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLL   +FIILKHIKSPSS KLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 1   MLIPSFSILLPSLLLLL--PIFIILKHIKSPSS-KLPLPPGPNPWPILGNLLQIGQNAHI 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLISLKLGAQRLIVASS AA TAVL+TQD LLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFSPE GDQWKNLRS+CKVNLFTAK IESQ  LREKKMKELV+FLESKQGSVVE+KDF
Sbjct: 121 SLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDF 180

Query: 181 VFTSVFKSLTNLI 194
           VFTSVF +L+NLI
Sbjct: 181 VFTSVFNTLSNLI 190

[Close]

BLAST of CSPI05G08400 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 8.6e-82
Identity = 170/193 (88.08%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLL   +FIILKHIKSPSS KLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 1   MLIPSFSILLPSLLLLL--PIFIILKHIKSPSS-KLPLPPGPNPWPILGNLLQIGQNAHI 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLISLKLGAQRLIVASS AA TAVL+TQD LLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFSPE GDQWKNLRS+CKVNLFTAK IESQ  LREKKMKELV+FLESKQGSVVE+KDF
Sbjct: 121 SLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDF 180

Query: 181 VFTSVFKSLTNLI 194
           VFTSVF +L+NLI
Sbjct: 181 VFTSVFNTLSNLI 190

[Close]

BLAST of CSPI05G08400 vs. ExPASy TrEMBL
Match: A0A0A0KPU9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173490 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.8e-79
Identity = 165/193 (85.49%), Postives = 172/193 (89.12%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPS LLLLLP +FIILK IKSPSSKKLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 11  MLIPSFSILLPS-LLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHI 70

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLIS KLGAQRL+VASS AA TAVL+TQD LLSARYIFQ+TPDRALHDQH
Sbjct: 71  SITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQH 130

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFS E GD WK LRSICKVNLFTAK  ESQ  LREKKMKELVEF+ESKQGSVVEIKDF
Sbjct: 131 SLVFSSECGDVWKKLRSICKVNLFTAKAFESQAILREKKMKELVEFVESKQGSVVEIKDF 190

Query: 181 VFTSVFKSLTNLI 194
           VFTS F  L+NLI
Sbjct: 191 VFTSAFNILSNLI 202

[Close]

BLAST of CSPI05G08400 vs. NCBI nr
Match: XP_031741238.1 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 384.0 bits (985), Expect = 8.2e-103
Identity = 200/211 (94.79%), Postives = 205/211 (97.16%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPP P+PWPI G+L  +GQNAH+
Sbjct: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPGPSPWPIFGNLLQMGQNAHV 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFAN+YGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANVYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFS EYGDQWKNLRSICKVNLFTAKEIESQ TLREKKMKELVEFLESKQGSVVEIKDF
Sbjct: 121 SLVFSHEYGDQWKNLRSICKVNLFTAKEIESQDTLREKKMKELVEFLESKQGSVVEIKDF 180

Query: 181 VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI 212
           VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI
Sbjct: 181 VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI 211

[Close]

BLAST of CSPI05G08400 vs. NCBI nr
Match: TYK00293.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 313.2 bits (801), Expect = 1.8e-81
Identity = 170/193 (88.08%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLL   +FIILKHIKSPSS KLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 1   MLIPSFSILLPSLLLLL--PIFIILKHIKSPSS-KLPLPPGPNPWPILGNLLQIGQNAHI 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLISLKLGAQRLIVASS AA TAVL+TQD LLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFSPE GDQWKNLRS+CKVNLFTAK IESQ  LREKKMKELV+FLESKQGSVVE+KDF
Sbjct: 121 SLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDF 180

Query: 181 VFTSVFKSLTNLI 194
           VFTSVF +L+NLI
Sbjct: 181 VFTSVFNTLSNLI 190

[Close]

BLAST of CSPI05G08400 vs. NCBI nr
Match: KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 313.2 bits (801), Expect = 1.8e-81
Identity = 170/193 (88.08%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLL   +FIILKHIKSPSS KLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 1   MLIPSFSILLPSLLLLL--PIFIILKHIKSPSS-KLPLPPGPNPWPILGNLLQIGQNAHI 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLISLKLGAQRLIVASS AA TAVL+TQD LLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFSPE GDQWKNLRS+CKVNLFTAK IESQ  LREKKMKELV+FLESKQGSVVE+KDF
Sbjct: 121 SLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDF 180

Query: 181 VFTSVFKSLTNLI 194
           VFTSVF +L+NLI
Sbjct: 181 VFTSVFNTLSNLI 190

[Close]

BLAST of CSPI05G08400 vs. NCBI nr
Match: XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 313.2 bits (801), Expect = 1.8e-81
Identity = 170/193 (88.08%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLL   +FIILKHIKSPSS KLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 1   MLIPSFSILLPSLLLLL--PIFIILKHIKSPSS-KLPLPPGPNPWPILGNLLQIGQNAHI 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLISLKLGAQRLIVASS AA TAVL+TQD LLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFSPE GDQWKNLRS+CKVNLFTAK IESQ  LREKKMKELV+FLESKQGSVVE+KDF
Sbjct: 121 SLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDF 180

Query: 181 VFTSVFKSLTNLI 194
           VFTSVF +L+NLI
Sbjct: 181 VFTSVFNTLSNLI 190

[Close]

BLAST of CSPI05G08400 vs. NCBI nr
Match: XP_031741076.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 307.0 bits (785), Expect = 1.3e-79
Identity = 166/193 (86.01%), Postives = 173/193 (89.64%), Query Frame = 0


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPS LLLLLP +FIILK IKSPSSKKLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 11  MLIPSFSILLPS-LLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHI 70

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLIS KLGAQRL+VASS AA TAVL+TQD LLSARYIFQ+TPDRALHDQH
Sbjct: 71  SITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQH 130

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFS E GD WK LRSICKVNLFTAK IESQ  LREKKMKELVEF+ESKQGSVVEIKDF
Sbjct: 131 SLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDF 190

Query: 181 VFTSVFKSLTNLI 194
           VFTS F  L+NLI
Sbjct: 191 VFTSAFNILSNLI 202

[Close]

BLAST of CSPI05G08400 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 100.9 bits (250), Expect = 1.3e-21
Identity = 66/188 (35.11%), Postives = 102/188 (54.26%), Query Frame = 0


Query: 14  LLLLLPSVFIILKHIKSP--SSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFANIYGP 73
           L   + S FII      P  S K +P PP P   PI+G++  +G+N H S    +  YGP
Sbjct: 12  LFCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGP 71

Query: 74  LISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRAL----HDQHSLVFSPE 133
           ++SLK G+   +V +S  A   VLRT D +LS+R     TP  ++    HD+ S+V+ P 
Sbjct: 72  IMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHDKVSVVWLPP 131

Query: 134 YGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFL--ESKQGSVVEIKDFVFTSV 193
              +W+ LR +    LF+ + IE+  TLRE K+KELV F+   S++   V+I    F + 
Sbjct: 132 SSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITA 191

[Close]

BLAST of CSPI05G08400 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-18
Identity = 60/185 (32.43%), Postives = 98/185 (52.97%), Query Frame = 0


Query: 13  LLLLLLPSVFIILKHIKS--PSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFANIYG 72
           LL   + S F+I    +S   S +   LPP P   PI+G++  +G+N H S    A IYG
Sbjct: 11  LLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYG 70

Query: 73  PLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQHSLVFSPEYGD 132
           P++SLK G    +V +S  A   VLRT D +LS R           H++ S+++ P    
Sbjct: 71  PIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFG-HEEVSVIWLPPSSA 130

Query: 133 QWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFL--ESKQGSVVEIKDFVFTSVFKS 192
           +W+ LR +    +F+ +  E+   LR KK++ELV F+   S++   V+I    +T+V   
Sbjct: 131 RWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNI 190

Query: 193 LTNLI 194
           ++N++
Sbjct: 191 ISNIL 194

[Close]

BLAST of CSPI05G08400 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 89.7 bits (221), Expect = 3.0e-18
Identity = 53/157 (33.76%), Postives = 87/157 (55.41%), Query Frame = 0


Query: 39  PPRPNPWPILGSLFHIGQNAHISITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQ 98
           PP P   PI+G++  +G+N H S T  +  YGP++SLKLG    +V +SR A   VL+T 
Sbjct: 39  PPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTH 98

Query: 99  DCLLSARYIFQMTPDRALHDQHSLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREK 158
           D +LS RYI + T     H + S+ +      +++ LR +    LF+ + I++   LR K
Sbjct: 99  DQILSGRYISEATKSNN-HHEFSVGWIHPSSSRFRMLRKLSATQLFSPQCIQATKALRMK 158

Query: 159 KMKELVEFLES--KQGSVVEIKDFVFTSVFKSLTNLI 194
           K++ELV FL    ++   V+I    F +    ++N++
Sbjct: 159 KVQELVNFLSESCEREEAVDISHVSFVTALNIISNIL 194

[Close]

BLAST of CSPI05G08400 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-17
Identity = 63/189 (33.33%), Postives = 98/189 (51.85%), Query Frame = 0


Query: 13  LLLLLLPSVFIILKHIKSP--SSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFANIYG 72
           LL   + S F+I    +S   S     LPP P   PI+G++  +G++ H S  + +  YG
Sbjct: 11  LLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYG 70

Query: 73  PLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRAL----HDQHSLVFSP 132
           P++SLKLG+   +V +S  A   VLRT D +LSAR     +P  A+    H   SLV+ P
Sbjct: 71  PVMSLKLGSLNTVVIASPEAAREVLRTHDQILSAR-----SPTNAVRSINHQDASLVWLP 130

Query: 133 EYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFL--ESKQGSVVEIKDFVFTS 192
               +W+ LR +    L + + IE+   LR  K+KELV F+   S +   V+I    F +
Sbjct: 131 SSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFIT 190

Query: 193 VFKSLTNLI 194
               ++N++
Sbjct: 191 TLNIISNIL 194

[Close]

BLAST of CSPI05G08400 vs. TAIR 10
Match: AT2G45560.2 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-17
Identity = 63/189 (33.33%), Postives = 98/189 (51.85%), Query Frame = 0


Query: 13  LLLLLLPSVFIILKHIKSP--SSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFANIYG 72
           LL   + S F+I    +S   S     LPP P   PI+G++  +G++ H S  + +  YG
Sbjct: 11  LLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYG 70

Query: 73  PLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRAL----HDQHSLVFSP 132
           P++SLKLG+   +V +S  A   VLRT D +LSAR     +P  A+    H   SLV+ P
Sbjct: 71  PVMSLKLGSLNTVVIASPEAAREVLRTHDQILSAR-----SPTNAVRSINHQDASLVWLP 130

Query: 133 EYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFL--ESKQGSVVEIKDFVFTS 192
               +W+ LR +    L + + IE+   LR  K+KELV F+   S +   V+I    F +
Sbjct: 131 SSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFIT 190

Query: 193 VFKSLTNLI 194
               ++N++
Sbjct: 191 TLNIISNIL 194

The following BLAST results are available for this feature:

BLAST of CSPI05G08400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64899	3.0e-31	42.77	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif...	[more]
O64900	9.6e-30	38.62	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3...	[more]
Q9SP06	4.8e-29	39.46	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum...	[more]
Q9FXW4	6.2e-29	43.01	Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3...	[more]
I3V6B1	1.4e-25	35.98	(S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum OX=3469...	[more]

BLAST of CSPI05G08400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KL66	4.0e-103	94.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173470 PE=4 SV=1	[more]
A0A5A7UIU3	8.6e-82	88.08	Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma...	[more]
A0A5D3BKL7	8.6e-82	88.08	Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma...	[more]
A0A1S3CT14	8.6e-82	88.08	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656...	[more]
A0A0A0KPU9	1.8e-79	85.49	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173490 PE=4 SV=1	[more]

BLAST of CSPI05G08400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031741238.1	8.2e-103	94.79	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus]	[more]
TYK00293.1	1.8e-81	88.08	putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku...	[more]
KAA0055170.1	1.8e-81	88.08	putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku...	[more]
XP_008467223.1	1.8e-81	88.08	PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel...	[more]
XP_031741076.1	1.3e-79	86.01	probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus]	[more]

BLAST of CSPI05G08400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45570.1	1.3e-21	35.11	cytochrome P450, family 76, subfamily C, polypeptide 2	[more]
AT2G45550.1	1.7e-18	32.43	cytochrome P450, family 76, subfamily C, polypeptide 4	[more]
AT1G33720.1	3.0e-18	33.76	cytochrome P450, family 76, subfamily C, polypeptide 6	[more]
AT2G45560.1	1.1e-17	33.33	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]
AT2G45560.2	1.1e-17	33.33	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..196 e-value: 4.8E-24 score: 86.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 34..193
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 39..193 e-value: 3.6E-16 score: 59.0
None	No IPR available	PANTHER	PTHR47956:SF23	(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKE	coord: 12..193
None	No IPR available	PANTHER	PTHR47956	CYTOCHROME P450 71B11-RELATED	coord: 12..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G08400.1	CSPI05G08400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen