CSPI05G02940 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G02940
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionGlucan endo-1,3-beta-glucosidase
LocationChr5: 3386649 .. 3388883 (-)
RNA-Seq ExpressionCSPI05G02940
SyntenyCSPI05G02940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAGCTTCTCCCAATCCCAAAGCAAAGCATCTGATCTGAATATGTGTTACTTCTTCAACAATGGCCAATGCCACACCCAAATTCACTATCTTCTTCCAAATCCATCTTCATCACCTCCATCCATCCCATGGCTTCCTCTCCTTTCACTCTCTCACACCACCTTCTTCTTCCTTACTTTTCCACTTTACCCACTTTTCTCCTTCTAATTCTCCCAACACTCTTCCAAATTCAAGGTAAATCAATTACATCATGCATTATTCTTATGTGCTTTTTTTCTTTTCAGTTTTCACTATCATATTTTTTCTTTATAAAAACATTTAACTTTCTTCAGGAGTTAATGGTGGATCAATCGGAGTCAATTACGGCACGGTGGCTGACAATCTCCCTCCGCCCTCTAAAGTTGCAGCCTTCCTTTTGGATAACACAATTATCGATCGCGTCAGACTCTTCGATGCGGATCCCGACATCTTACGAGCTTTCGCTCATACCGGCATTTCAGTATCCATTACAATCCCTAATGATCAAATACCCCGCTTAGTGAAGCCAAATTTCGCTGAAGAATGGATCAAATTCAACATTCAACCCTACATCCCTGCCACAAACATCATTCGCGTTCTAGTTGGCAATGAAGTCTTATCCACCGCCAATAAATTGCTCATTGCCAACCTCGTTCCAGCAATGCAGAGACTCCACACAGCTCTCATTGAAACCTCCTTGGATAGAAGGATTCAAATCTCAACGCCCCACTCGCTCGGCATTTTGTCGAACTCAACCCCGCCGTCGACTGCAAGGTTCAGGCAAGGCTACGATACACACGTAATTAAACCAATGCTGAGCTTCCTAAGAGAAACAAACTCGCCATTAATGGTGAATCCATATCCATTCTTCGCCTGCACTGCTGACAATCTAGATTACGCGCTCTTCCGGCCGAACCCCGGCGTGTTCGACTCGGATTTAGGAATTCTATACACCAATATGTTGGATGCTCAATTGGACGCGGTTTATTCCGCGATGAAAAGCTTGGGCTTTGAGGATCTCGATATTGTGATAGCCGAAACGGGCTGGCCTTCAAAGGGTGATCCAACCCAAGTCGGAGTCGGCCCAAAAGAAGCTGCTTATTACAATGGGAATCTGATGCGACATGTCGTATCAGGCAAAGGGACGCCGTTGATGCCGAACCGGACTTTCGAGACATACATTTTCGCTTTATTCAATGAGAATCTCAAACCGGGTCCAATTGGCGAAAGGAACTTTGGGCTATTCGAACCAGATCTGAGCCCGGTTTATGAGATTGGGATTCTTCGGCCCACGGTAGCTGCCACAGCTCGAACCCACCCTAAAACGTTTTTGATAATCATGATGGTTAGTGTTACATGTTGGTTGATAGGTTTGTTGTGGACTTGGATCTAACAGGCTCAATCCGCTACTCCGAGAGCCCATCAAGGTCAAGTTGAAGGCCCAAGCCCAAGCACAGTGAGTGAAAGTAAAAGGTGGTGCCTGCCCAAAAGTGAGGCCAGCGAGGAGGGCTTGCAGAGAAACATAGATTACGTTTGTGGGTTGGGCTTGGACTGTGGGCCGATAAAAGAAAATGGGGCGTGTTTTGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAACGATTTTGATTGCGATTTTGACCAAACCGGCACTTTAACCACGGTGGATCCAAGTAAGTCCCATAAGATTCTTATTTTTATTCTCATTCTCATTGATTTTTTTTTAATTAGTCAAAATTCATTGACTAATTCTGTTGTAATTTAATTATTCACAAAGGTATTTTCTTTAGCAATTTGTTTTTGTTTTAATTTTCCAGGTTATGGGAAGTGCAAATATTGGTAAAATGGGAGATTTGGAGGGAGCTCAACCCAATCACCCCTATTTCCTTTCTCTTATTACTTATTTTTGTTTGTTTCCTTTGTATTATTTAGTTTAGCAAAAAAAAAAGAAAAAAAGTTATTTAGTTACGCAGTTGAAGAGGTGGAGAAGGTGAGATGTTTAGAGATAGATTCGTGAGTTCTATCTCCAATCCAAAAGTCTTTTCTTGTTATGTACTATCTCTAGTCCATAATTTAAGTTTAGGGTATTTTGTGGGTATCTCTTCAACCTATTAACTCTTTGTGGGTTTTTGTTTTTAACTCAGATCATGTAATAGTTTTGATTTGGAAATGGTTTCTTCAGCGG

mRNA sequence

TAAGCTTCTCCCAATCCCAAAGCAAAGCATCTGATCTGAATATGTGTTACTTCTTCAACAATGGCCAATGCCACACCCAAATTCACTATCTTCTTCCAAATCCATCTTCATCACCTCCATCCATCCCATGGCTTCCTCTCCTTTCACTCTCTCACACCACCTTCTTCTTCCTTACTTTTCCACTTTACCCACTTTTCTCCTTCTAATTCTCCCAACACTCTTCCAAATTCAAGGAGTTAATGGTGGATCAATCGGAGTCAATTACGGCACGGTGGCTGACAATCTCCCTCCGCCCTCTAAAGTTGCAGCCTTCCTTTTGGATAACACAATTATCGATCGCGTCAGACTCTTCGATGCGGATCCCGACATCTTACGAGCTTTCGCTCATACCGGCATTTCAGTATCCATTACAATCCCTAATGATCAAATACCCCGCTTAGTGAAGCCAAATTTCGCTGAAGAATGGATCAAATTCAACATTCAACCCTACATCCCTGCCACAAACATCATTCGCGTTCTAGTTGGCAATGAAGTCTTATCCACCGCCAATAAATTGCTCATTGCCAACCTCGTTCCAGCAATGCAGAGACTCCACACAGCTCTCATTGAAACCTCCTTGGATAGAAGGATTCAAATCTCAACGCCCCACTCGCTCGGCATTTTGTCGAACTCAACCCCGCCGTCGACTGCAAGGTTCAGGCAAGGCTACGATACACACGTAATTAAACCAATGCTGAGCTTCCTAAGAGAAACAAACTCGCCATTAATGGTGAATCCATATCCATTCTTCGCCTGCACTGCTGACAATCTAGATTACGCGCTCTTCCGGCCGAACCCCGGCGTGTTCGACTCGGATTTAGGAATTCTATACACCAATATGTTGGATGCTCAATTGGACGCGGTTTATTCCGCGATGAAAAGCTTGGGCTTTGAGGATCTCGATATTGTGATAGCCGAAACGGGCTGGCCTTCAAAGGGTGATCCAACCCAAGTCGGAGTCGGCCCAAAAGAAGCTGCTTATTACAATGGGAATCTGATGCGACATGTCGTATCAGGCAAAGGGACGCCGTTGATGCCGAACCGGACTTTCGAGACATACATTTTCGCTTTATTCAATGAGAATCTCAAACCGGGTCCAATTGGCGAAAGGAACTTTGGGCTATTCGAACCAGATCTGAGCCCGGTTTATGAGATTGGGATTCTTCGGCCCACGGCTCAATCCGCTACTCCGAGAGCCCATCAAGGTCAAGTTGAAGGCCCAAGCCCAAGCACAGTGAGTGAAAGTAAAAGGTGGTGCCTGCCCAAAAGTGAGGCCAGCGAGGAGGGCTTGCAGAGAAACATAGATTACGTTTGTGGGTTGGGCTTGGACTGTGGGCCGATAAAAGAAAATGGGGCGTGTTTTGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAACGATTTTGATTGCGATTTTGACCAAACCGGCACTTTAACCACGGTGGATCCAAGTTATGGGAAGTGCAAATATTGGTAAAATGGGAGATTTGGAGGGAGCTCAACCCAATCACCCCTATTTCCTTTCTCTTATTACTTATTTTTGTTTGTTTCCTTTGTATTATTTAGTTTAGCAAAAAAAAAAGAAAAAAAGTTATTTAGTTACGCAGTTGAAGAGGTGGAGAAGGTGAGATGTTTAGAGATAGATTCGTGAGTTCTATCTCCAATCCAAAAGTCTTTTCTTGTTATGTACTATCTCTAGTCCATAATTTAAGTTTAGGGTATTTTGTGGGTATCTCTTCAACCTATTAACTCTTTGTGGGTTTTTGTTTTTAACTCAGATCATGTAATAGTTTTGATTTGGAAATGGTTTCTTCAGCGG

Coding sequence (CDS)

ATGCCACACCCAAATTCACTATCTTCTTCCAAATCCATCTTCATCACCTCCATCCATCCCATGGCTTCCTCTCCTTTCACTCTCTCACACCACCTTCTTCTTCCTTACTTTTCCACTTTACCCACTTTTCTCCTTCTAATTCTCCCAACACTCTTCCAAATTCAAGGAGTTAATGGTGGATCAATCGGAGTCAATTACGGCACGGTGGCTGACAATCTCCCTCCGCCCTCTAAAGTTGCAGCCTTCCTTTTGGATAACACAATTATCGATCGCGTCAGACTCTTCGATGCGGATCCCGACATCTTACGAGCTTTCGCTCATACCGGCATTTCAGTATCCATTACAATCCCTAATGATCAAATACCCCGCTTAGTGAAGCCAAATTTCGCTGAAGAATGGATCAAATTCAACATTCAACCCTACATCCCTGCCACAAACATCATTCGCGTTCTAGTTGGCAATGAAGTCTTATCCACCGCCAATAAATTGCTCATTGCCAACCTCGTTCCAGCAATGCAGAGACTCCACACAGCTCTCATTGAAACCTCCTTGGATAGAAGGATTCAAATCTCAACGCCCCACTCGCTCGGCATTTTGTCGAACTCAACCCCGCCGTCGACTGCAAGGTTCAGGCAAGGCTACGATACACACGTAATTAAACCAATGCTGAGCTTCCTAAGAGAAACAAACTCGCCATTAATGGTGAATCCATATCCATTCTTCGCCTGCACTGCTGACAATCTAGATTACGCGCTCTTCCGGCCGAACCCCGGCGTGTTCGACTCGGATTTAGGAATTCTATACACCAATATGTTGGATGCTCAATTGGACGCGGTTTATTCCGCGATGAAAAGCTTGGGCTTTGAGGATCTCGATATTGTGATAGCCGAAACGGGCTGGCCTTCAAAGGGTGATCCAACCCAAGTCGGAGTCGGCCCAAAAGAAGCTGCTTATTACAATGGGAATCTGATGCGACATGTCGTATCAGGCAAAGGGACGCCGTTGATGCCGAACCGGACTTTCGAGACATACATTTTCGCTTTATTCAATGAGAATCTCAAACCGGGTCCAATTGGCGAAAGGAACTTTGGGCTATTCGAACCAGATCTGAGCCCGGTTTATGAGATTGGGATTCTTCGGCCCACGGCTCAATCCGCTACTCCGAGAGCCCATCAAGGTCAAGTTGAAGGCCCAAGCCCAAGCACAGTGAGTGAAAGTAAAAGGTGGTGCCTGCCCAAAAGTGAGGCCAGCGAGGAGGGCTTGCAGAGAAACATAGATTACGTTTGTGGGTTGGGCTTGGACTGTGGGCCGATAAAAGAAAATGGGGCGTGTTTTGCTCCTAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCCTACTTTCAAGCCACGGAAGGGAACGATTTTGATTGCGATTTTGACCAAACCGGCACTTTAACCACGGTGGATCCAAGTTATGGGAAGTGCAAATATTGGTAA

Protein sequence

MPHPNSLSSSKSIFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKYW*
Homology
BLAST of CSPI05G02940 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 5.5e-129
Identity = 227/437 (51.95%), Postives = 303/437 (69.34%), Query Frame = 0

Query: 62  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQI 121
           IGVNYG  ADNLP P+ VA FL   T IDRV+LFDA+P  + AFA T IS+++++PN  +
Sbjct: 26  IGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFISAFAGTPISLAVSLPNSAL 85

Query: 122 PRLVKP----NFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHT 181
           P L       + A  WI+ N+ PY+PATN+  +L GNE+L + +  LI +L+PAM+RL  
Sbjct: 86  PALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILLSTDTNLILSLLPAMRRLAQ 145

Query: 182 ALIETSLDRRIQISTPHSLGILSNSTP-PSTARFRQGYDTHVIKPMLSFLRETNSPLMVN 241
           AL    L   ++++TPH LGIL+ S   PS A FR GY+T +   ML F R+T SP MVN
Sbjct: 146 ALKAEGL-TGVRVTTPHYLGILAPSDGIPSNASFRAGYNTKLFPAMLQFHRDTGSPFMVN 205

Query: 242 PYPFFACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 301
           PYP+F+   + L+YALFRPN G++D    + YT+MLDAQ+DA+Y+AMK LG+ D+DI + 
Sbjct: 206 PYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAMKKLGYGDVDIAVG 265

Query: 302 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPG 361
           E GWP++ +P Q+GVG +EA  +N  ++R   SGKGTPLMPNRTFETY+F+LF+EN KPG
Sbjct: 266 EAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDENQKPG 325

Query: 362 PIGERNFGLFEPDLSPVYEIGILRPTAQSA-TPRAHQGQVEGPSPSTVSESKRWCLPKSE 421
           PI ER+FGLF PD +PVY++G+LR  A  A TP  +      P P+  S   +WC+ K  
Sbjct: 326 PIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPSPNPSPNPSPKPAP-SGGGKWCVAKDG 385

Query: 422 ASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFD 481
           A+   LQ NI+Y CG  +DC PI+  GACF+PN+++AHA+YVMNAY+QA    D  CDF 
Sbjct: 386 ANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTDLACDFK 445

Query: 482 QTGTLTTVDPSYGKCKY 493
            TG +T+ DPSYG CKY
Sbjct: 446 GTGIVTSSDPSYGGCKY 459

BLAST of CSPI05G02940 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 428.3 bits (1100), Expect = 1.2e-118
Identity = 215/436 (49.31%), Postives = 286/436 (65.60%), Query Frame = 0

Query: 62  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQI 121
           IGVNYG VADNLPPPS+    LL +T I +VRL+ ADP I++A A TG+ + I   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVK-LLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 122 PRLVK-PNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALI 181
           P L   PN A +WI  N+ P+ PA+ I+ + VGNE+L + +  L+  L+PAMQ +  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 182 ETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 241
             SL  +I++ST +S+ +L +S PPS+  F  GY T  +K +L FL +T SP  +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 242 FACTAD----NLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 301
           FA  +D     L + LF PN G  DS  GI YTNM DAQ+DAV+SA+KS+GFE ++IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 302 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPG 361
           ETGW S+GD  +VG     A  YNGNL+ H+ S  GTPLMP +  +TYIFAL++ENLKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 362 PIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEA 421
           P  ER FGLF+ DLS VY++G+ + ++ S TP    G+V          S  WC+PK  A
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSSQTP---SGKV---------TSSGWCVPKKGA 385

Query: 422 SEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQ 481
           + E LQ ++D+ CG G+DCG I+  GACF PN V +HAAY MN YFQ +     DCDF +
Sbjct: 386 TNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSK 445

Query: 482 TGTLTTVDPSYGKCKY 493
           T T+T+ +PSY  C Y
Sbjct: 446 TATVTSQNPSYNNCVY 447

BLAST of CSPI05G02940 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.1e-113
Identity = 206/457 (45.08%), Postives = 293/457 (64.11%), Query Frame = 0

Query: 42  TFLLLILPTLFQIQGVNGGS-IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPD 101
           + L L+   L      N  S +GVNYG ++DNL P  +    LL +T I +VRLF A+P 
Sbjct: 9   SLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNL-PSLQATVNLLKSTTIQKVRLFGAEPA 68

Query: 102 ILRAFAHTGISVSITIPNDQIPRLV-KPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLST 161
           +++AFA+TG+ + I   N  IP L   PN A +++K N+  + PA+NII + VGNEVL++
Sbjct: 69  VIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTS 128

Query: 162 ANKLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVI 221
            ++ LI+ L+PAMQ +  AL   SL  ++++ST H++ +LS S PPS+  F  G     +
Sbjct: 129 GDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLG-DTM 188

Query: 222 KPMLSFLRETNSPLMVNPYPFFAC----TADNLDYALFRPNPGVFDSDLGILYTNMLDAQ 281
           K +L F    ++P M++PYP+FA     T D L + LF+PN G  DS  G  YTNM DAQ
Sbjct: 189 KALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQ 248

Query: 282 LDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPL 341
           +DAV+SA+ ++GF+D++IV+AETGWP  GD  +VG     A  Y GNL+ H+ S  GTPL
Sbjct: 249 VDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPL 308

Query: 342 MPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQV 401
           MP ++ +TY+F+L++E+ K G   E+ FGLF+PD S  Y++G+L+ T    TP       
Sbjct: 309 MPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPAT----- 368

Query: 402 EGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAA 461
             P+P T   +  WC+PK   S++ L  NI+Y CG G+DCGPI+  GACF PNTV+AHAA
Sbjct: 369 --PTP-TPKAAGSWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAA 428

Query: 462 YVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 493
           YVMN Y+Q+   N ++CDF QT TLT  +PSYG C +
Sbjct: 429 YVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNF 455

BLAST of CSPI05G02940 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.4e-92
Identity = 179/455 (39.34%), Postives = 265/455 (58.24%), Query Frame = 0

Query: 62  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQI 121
           IG+ YG  ADNLP P++V+  L+ +  I  VR++DA+ D+L+AFA+TGI + I +PN  +
Sbjct: 27  IGICYGRNADNLPSPNRVSE-LIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADL 86

Query: 122 PRLVK-PNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALI 181
               +  +  + W+  NI PY P+T I  + VG EV    +      ++PAM+ +HTAL 
Sbjct: 87  LAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDN-ATGLVLPAMRNIHTALK 146

Query: 182 ETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 241
           ++ LD++I+IS+ HSL ILS S PPS+A F + +    +KPML FL E  SP M++ YP+
Sbjct: 147 KSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSA-FLKPMLEFLVENESPFMIDLYPY 206

Query: 242 FACTADN----LDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 301
           +A         L+YALF  +  V D   G+LY+NM DAQLDA+Y A+ ++ F+ + +++ 
Sbjct: 207 YAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVT 266

Query: 302 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPG 361
           E+GWPSKG P +    P+ A  YN NL+RHV+   GTP  P    + Y+F+LFNEN KPG
Sbjct: 267 ESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPG 326

Query: 362 PIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPSTVSE----------- 421
              ERN+G+F  + + VY +     T ++ TP +      G SPS  S            
Sbjct: 327 IESERNWGMFYANGTNVYALDF---TGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTI 386

Query: 422 -------SKRWCLPKSEASEEGLQRNIDYVCGLG-LDCGPIKENGACFAPNTVRAHAAYV 481
                  +K+WC+  S+AS   LQ  +D+ CG G +DC  ++ +  CF P+TV +HA+Y 
Sbjct: 387 GGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYA 446

Query: 482 MNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 493
            N Y+Q +  +  DC F+        DPSYG C Y
Sbjct: 447 FNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of CSPI05G02940 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 334.3 bits (856), Expect = 2.3e-90
Identity = 185/459 (40.31%), Postives = 270/459 (58.82%), Query Frame = 0

Query: 44  LLLILPTLFQIQGVNGGS-IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDIL 103
           LLL+   LF    ++  S IGVN GT   N+P P++V A LL +  I+RVRL+DAD  +L
Sbjct: 4   LLLLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVA-LLKSQNINRVRLYDADRSML 63

Query: 104 RAFAHTGISVSITIPNDQIPRLVKPN-FAEEWIKFNIQPYIPATNIIRVLVGNEVLSTAN 163
            AFAHTG+ V I++PNDQ+  + + N  A  W+  N+  Y PATNI  + VG+EVL++  
Sbjct: 64  LAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLT 123

Query: 164 KLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKP 223
               + LV A++ +  AL+  +LDR+I++STPHS  I+ +S PPS A F + +D  VI P
Sbjct: 124 N-AASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDP-VIVP 183

Query: 224 MLSFLRETNSPLMVNPYPFFACTADN----LDYALFRP---NPGVFDSDLGILYTNMLDA 283
           +L FL+ T SPL++N YP+F     N    LDYALF+P   N    D++  + YTN+ DA
Sbjct: 184 LLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDA 243

Query: 284 QLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTP 343
            +DA Y AM  L F ++ IV+ E+GWPSKG P++     + A  YN NL++HV++  GTP
Sbjct: 244 IVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTP 303

Query: 344 LMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQ 403
             P     TYI+ L+NE+ +PGP+ E+N+GLF  + +PVY + +    A  A    +Q  
Sbjct: 304 KHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQ-- 363

Query: 404 VEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLG-LDCGPIKENGACFAPNTVRAH 463
                         +C+ K +   + LQ  +D+ CG G +DC  + +  +C+ P+ V AH
Sbjct: 364 -------------TFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAH 423

Query: 464 AAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 493
           + Y  NAY+Q        CDF    T+TT DPS G C +
Sbjct: 424 STYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTCVF 444

BLAST of CSPI05G02940 vs. ExPASy TrEMBL
Match: A0A0A0KJS0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.2e-289
Identity = 491/493 (99.59%), Postives = 491/493 (99.59%), Query Frame = 0

Query: 1   MPHPNSLSSSKSIFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGG 60
           MPHPNSLSSSKSIFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGG
Sbjct: 1   MPHPNSLSSSKSIFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGG 60

Query: 61  SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ 120
           SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ
Sbjct: 61  SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ 120

Query: 121 IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALI 180
           IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQ LHTALI
Sbjct: 121 IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALI 180

Query: 181 ETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 240
           E SLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF
Sbjct: 181 EASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 240

Query: 241 FACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGW 300
           FACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGW
Sbjct: 241 FACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGW 300

Query: 301 PSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGE 360
           PSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGE
Sbjct: 301 PSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGE 360

Query: 361 RNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEG 420
           RNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEG
Sbjct: 361 RNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEG 420

Query: 421 LQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTL 480
           LQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTL
Sbjct: 421 LQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTL 480

Query: 481 TTVDPSYGKCKYW 494
           TTVDPSYGKCKYW
Sbjct: 481 TTVDPSYGKCKYW 493

BLAST of CSPI05G02940 vs. ExPASy TrEMBL
Match: A0A1S3AV36 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 8.2e-269
Identity = 459/500 (91.80%), Postives = 473/500 (94.60%), Query Frame = 0

Query: 1   MPHPNSLSSSKSIFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGG 60
           MPHPNS SSSK I +  +HPMAS PFT SHHLLLPYFSTLPTFLLLILPTL QIQGVNGG
Sbjct: 1   MPHPNSPSSSK-IHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGG 60

Query: 61  SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ 120
           SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ
Sbjct: 61  SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ 120

Query: 121 IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALI 180
           IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQ LHTAL+
Sbjct: 121 IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALV 180

Query: 181 ETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 240
           E SLDRRIQISTPHSLGILSNSTPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF
Sbjct: 181 EASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 240

Query: 241 FACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGW 300
           FACTA+NLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKS+ FEDLDIVIAETGW
Sbjct: 241 FACTAENLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGW 300

Query: 301 PSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGE 360
           PSKGDP+QVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGP+GE
Sbjct: 301 PSKGDPSQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPVGE 360

Query: 361 RNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEG-------PSPSTVSESKRWCLPK 420
           RNFGLFEPDL+PVYEIGILRPTAQSATPRAHQG VEG       PS STVSESKRWC+PK
Sbjct: 361 RNFGLFEPDLTPVYEIGILRPTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPK 420

Query: 421 SEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCD 480
           +EAS E LQRNIDYVCGLGLDCGPI+ENG C+APNTVR HAAYVMNAYFQATEG +FDCD
Sbjct: 421 TEASVEALQRNIDYVCGLGLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCD 480

Query: 481 FDQTGTLTTVDPSYGKCKYW 494
           FDQTG LTTVDPSYGKC+YW
Sbjct: 481 FDQTGALTTVDPSYGKCRYW 499

BLAST of CSPI05G02940 vs. ExPASy TrEMBL
Match: A0A5A7TGS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00250 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 3.6e-256
Identity = 438/472 (92.80%), Postives = 450/472 (95.34%), Query Frame = 0

Query: 21  MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 80
           MAS PFT SHHLLLPYFSTLPTFLLLILPTL QIQGVNGGSIGVNYGTVADNLPPPSKVA
Sbjct: 1   MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 60

Query: 81  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 140
           AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP
Sbjct: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120

Query: 141 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILS 200
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQ LHTAL+E SLDRRIQISTPHSLGILS
Sbjct: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 180

Query: 201 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 260
           NSTPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTA+NLDYALFRPNPGVF
Sbjct: 181 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 240

Query: 261 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 320
           DSDLGILYTNMLDAQLDAVYSAMKS+ FEDLDIVIAETGWPSKGDP+QVGVGPKEAAYYN
Sbjct: 241 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 300

Query: 321 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 380
           GNLMRHVV+GKGTPLMPNRTFETYIFALFNENLKPGP+GERNFGLFEPDL+PVYEIGILR
Sbjct: 301 GNLMRHVVTGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 360

Query: 381 PTAQSATPRAHQGQVEG-------PSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGL 440
           PTAQSATPRAHQG VEG       PS STVSESKRWC+PK+EAS E LQRNIDYVCGLGL
Sbjct: 361 PTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEASVEALQRNIDYVCGLGL 420

Query: 441 DCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDP 486
           DCGPI+ENG C+APNTVR HAAYVMNAYFQATEG +FDCDFDQTG LTTVDP
Sbjct: 421 DCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVDP 472

BLAST of CSPI05G02940 vs. ExPASy TrEMBL
Match: A0A6J1H2P3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.5e-233
Identity = 402/476 (84.45%), Postives = 431/476 (90.55%), Query Frame = 0

Query: 21  MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 80
           MASS F LS  LL  Y S   T L+L+LPTL Q+Q   GGSIGVNYGT+ADNLP PSKVA
Sbjct: 1   MASS-FALS-RLLRAYTSIAATLLILLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVA 60

Query: 81  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 140
            FLLD+TIIDR+RLFDA+PDILRAFAHT ISV+IT+PNDQIPRLVKPNFAEEWIKFN+QP
Sbjct: 61  EFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQP 120

Query: 141 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILS 200
           Y+PAT+IIR+LVGNEVLSTANK+LIANLVPAMQ LHTAL E SL+RRIQ+STPHSLGILS
Sbjct: 121 YVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNRRIQVSTPHSLGILS 180

Query: 201 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 260
           NS+PPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFF  TA+ LDYALFRPNPGV+
Sbjct: 181 NSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVY 240

Query: 261 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 320
           DSD GILYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDP Q+GVGPKEAA YN
Sbjct: 241 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYN 300

Query: 321 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 380
           GNL+RHVVSG GTPLMPNRTFETYIFALFNENLKPGP+GERNFGLFEPDLSPVYEIGILR
Sbjct: 301 GNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILR 360

Query: 381 PTAQSATPRAHQG---QVEGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGP 440
           PTAQS+TPRAH G     +GPSPS V++ KRWCLPK+EA  E LQRNIDYVCGLGLDCGP
Sbjct: 361 PTAQSSTPRAHHGPEPTPKGPSPSPVADGKRWCLPKTEADSEALQRNIDYVCGLGLDCGP 420

Query: 441 IKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKYW 494
           I+ENG CF PNTVRAHAAYVMNAYFQAT+GND DCDF QTG LTTVDPSYGKCKYW
Sbjct: 421 IQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPSYGKCKYW 473

BLAST of CSPI05G02940 vs. ExPASy TrEMBL
Match: A0A6J1K780 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.9e-229
Identity = 403/500 (80.60%), Postives = 436/500 (87.20%), Query Frame = 0

Query: 3   HPNSLSSS------KSIFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQG 62
           H N  SS+       S+F  S  PMASS F LS  LL  Y S   T LLL+LPTL Q+Q 
Sbjct: 16  HQNVTSSAMANATLNSLFSLS-SPMASS-FALS-RLLRAYTSIAVTLLLLLLPTLLQMQ- 75

Query: 63  VNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITI 122
             GGSIGVNYGT+ADNLP PSKVA FLLD+TII R+RLFDA+PDILRAFAHT ISV+IT+
Sbjct: 76  AKGGSIGVNYGTLADNLPLPSKVAEFLLDSTIIGRIRLFDANPDILRAFAHTRISVTITV 135

Query: 123 PNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLH 182
           PNDQIPRLVKPNFAEEWIKFN+QPY+PAT+IIR+LVGNEVLSTANK+LIANLVPAMQ LH
Sbjct: 136 PNDQIPRLVKPNFAEEWIKFNVQPYVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLH 195

Query: 183 TALIETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVN 242
           TAL E SL+ RIQ+STPHSLGILSNS+PPSTARFRQGYDTHVIKPMLSFL+ETNSPLMVN
Sbjct: 196 TALSEASLNSRIQVSTPHSLGILSNSSPPSTARFRQGYDTHVIKPMLSFLKETNSPLMVN 255

Query: 243 PYPFFACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 302
           PYPFF  TA+ LDYALFRPNPGV+DSD GILYTNMLDAQLDAVYSAMKSLGF DLDIVIA
Sbjct: 256 PYPFFGTTAETLDYALFRPNPGVYDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIA 315

Query: 303 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPG 362
           ETGWPSKGDP Q+GVGPKEAA YNGNL+RHVVSG GTPLMPNRTFETYIFALFNE+LKPG
Sbjct: 316 ETGWPSKGDPAQIGVGPKEAADYNGNLLRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 375

Query: 363 PIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQG---QVEGPSPSTVSESKRWCLPK 422
           P+GERNFGLFEPDL+PVYEIGILRPTAQS+TPRAH G     +GPSPS V++ KRWCLPK
Sbjct: 376 PVGERNFGLFEPDLNPVYEIGILRPTAQSSTPRAHHGPEPTPKGPSPSPVADGKRWCLPK 435

Query: 423 SEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCD 482
           +EA  E LQRNIDYVCGLG DCGPI+ NG CF PNTVRAHAAYVMNAYFQA +GND DCD
Sbjct: 436 TEADSEALQRNIDYVCGLGFDCGPIQANGPCFLPNTVRAHAAYVMNAYFQAIDGNDSDCD 495

Query: 483 FDQTGTLTTVDPSYGKCKYW 494
           F QTG LTTVDPSYGKCKYW
Sbjct: 496 FQQTGDLTTVDPSYGKCKYW 511

BLAST of CSPI05G02940 vs. NCBI nr
Match: XP_011654592.1 (glucan endo-1,3-beta-glucosidase isoform X1 [Cucumis sativus])

HSP 1 Score: 1003.0 bits (2592), Expect = 8.7e-289
Identity = 491/493 (99.59%), Postives = 491/493 (99.59%), Query Frame = 0

Query: 1   MPHPNSLSSSKSIFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGG 60
           MPHPNSLSSSKSIFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGG
Sbjct: 1   MPHPNSLSSSKSIFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGG 60

Query: 61  SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ 120
           SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ
Sbjct: 61  SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ 120

Query: 121 IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALI 180
           IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQ LHTALI
Sbjct: 121 IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALI 180

Query: 181 ETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 240
           E SLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF
Sbjct: 181 EASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 240

Query: 241 FACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGW 300
           FACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGW
Sbjct: 241 FACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGW 300

Query: 301 PSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGE 360
           PSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGE
Sbjct: 301 PSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGE 360

Query: 361 RNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEG 420
           RNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEG
Sbjct: 361 RNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEG 420

Query: 421 LQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTL 480
           LQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTL
Sbjct: 421 LQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTL 480

Query: 481 TTVDPSYGKCKYW 494
           TTVDPSYGKCKYW
Sbjct: 481 TTVDPSYGKCKYW 493

BLAST of CSPI05G02940 vs. NCBI nr
Match: KAE8647796.1 (hypothetical protein Csa_000195 [Cucumis sativus])

HSP 1 Score: 954.1 bits (2465), Expect = 4.6e-274
Identity = 471/490 (96.12%), Postives = 471/490 (96.12%), Query Frame = 0

Query: 21  MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 80
           MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA
Sbjct: 1   MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 60

Query: 81  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 140
           AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP
Sbjct: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120

Query: 141 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILS 200
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQ LHTALIE SLDRRIQISTPHSLGILS
Sbjct: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILS 180

Query: 201 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 260
           NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF
Sbjct: 181 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 240

Query: 261 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 320
           DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN
Sbjct: 241 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 300

Query: 321 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 380
           GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR
Sbjct: 301 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 360

Query: 381 PT-----------------AQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQR 440
           PT                 AQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQR
Sbjct: 361 PTVAATARTHPKTFLIIMMAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEASEEGLQR 420

Query: 441 NIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTV 494
           NIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTV
Sbjct: 421 NIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTV 480

BLAST of CSPI05G02940 vs. NCBI nr
Match: XP_008437641.1 (PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo])

HSP 1 Score: 935.6 bits (2417), Expect = 1.7e-268
Identity = 459/500 (91.80%), Postives = 473/500 (94.60%), Query Frame = 0

Query: 1   MPHPNSLSSSKSIFITSIHPMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGG 60
           MPHPNS SSSK I +  +HPMAS PFT SHHLLLPYFSTLPTFLLLILPTL QIQGVNGG
Sbjct: 1   MPHPNSPSSSK-IHLHHLHPMASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGG 60

Query: 61  SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ 120
           SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ
Sbjct: 61  SIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQ 120

Query: 121 IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALI 180
           IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQ LHTAL+
Sbjct: 121 IPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALV 180

Query: 181 ETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 240
           E SLDRRIQISTPHSLGILSNSTPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF
Sbjct: 181 EASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 240

Query: 241 FACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGW 300
           FACTA+NLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKS+ FEDLDIVIAETGW
Sbjct: 241 FACTAENLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGW 300

Query: 301 PSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGE 360
           PSKGDP+QVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGP+GE
Sbjct: 301 PSKGDPSQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPVGE 360

Query: 361 RNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEG-------PSPSTVSESKRWCLPK 420
           RNFGLFEPDL+PVYEIGILRPTAQSATPRAHQG VEG       PS STVSESKRWC+PK
Sbjct: 361 RNFGLFEPDLTPVYEIGILRPTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPK 420

Query: 421 SEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCD 480
           +EAS E LQRNIDYVCGLGLDCGPI+ENG C+APNTVR HAAYVMNAYFQATEG +FDCD
Sbjct: 421 TEASVEALQRNIDYVCGLGLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCD 480

Query: 481 FDQTGTLTTVDPSYGKCKYW 494
           FDQTG LTTVDPSYGKC+YW
Sbjct: 481 FDQTGALTTVDPSYGKCRYW 499

BLAST of CSPI05G02940 vs. NCBI nr
Match: KAA0042530.1 (glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa] >TYK05933.1 glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa])

HSP 1 Score: 893.6 bits (2308), Expect = 7.4e-256
Identity = 438/472 (92.80%), Postives = 450/472 (95.34%), Query Frame = 0

Query: 21  MASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVA 80
           MAS PFT SHHLLLPYFSTLPTFLLLILPTL QIQGVNGGSIGVNYGTVADNLPPPSKVA
Sbjct: 1   MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 60

Query: 81  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 140
           AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP
Sbjct: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120

Query: 141 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILS 200
           YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQ LHTAL+E SLDRRIQISTPHSLGILS
Sbjct: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 180

Query: 201 NSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVF 260
           NSTPPST RFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTA+NLDYALFRPNPGVF
Sbjct: 181 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 240

Query: 261 DSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYN 320
           DSDLGILYTNMLDAQLDAVYSAMKS+ FEDLDIVIAETGWPSKGDP+QVGVGPKEAAYYN
Sbjct: 241 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 300

Query: 321 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILR 380
           GNLMRHVV+GKGTPLMPNRTFETYIFALFNENLKPGP+GERNFGLFEPDL+PVYEIGILR
Sbjct: 301 GNLMRHVVTGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 360

Query: 381 PTAQSATPRAHQGQVEG-------PSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGL 440
           PTAQSATPRAHQG VEG       PS STVSESKRWC+PK+EAS E LQRNIDYVCGLGL
Sbjct: 361 PTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEASVEALQRNIDYVCGLGL 420

Query: 441 DCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDP 486
           DCGPI+ENG C+APNTVR HAAYVMNAYFQATEG +FDCDFDQTG LTTVDP
Sbjct: 421 DCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVDP 472

BLAST of CSPI05G02940 vs. NCBI nr
Match: XP_038906871.1 (glucan endo-1,3-beta-glucosidase [Benincasa hispida])

HSP 1 Score: 857.1 bits (2213), Expect = 7.7e-245
Identity = 423/483 (87.58%), Postives = 441/483 (91.30%), Query Frame = 0

Query: 20  PMASSPFTLSHHLLLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKV 79
           P  +SPFT S HLL  + ST+ TFLLL LPTL QIQG   GSIGVNYGT+ADNLPPPSKV
Sbjct: 28  PPMASPFTPS-HLLFVHISTVSTFLLL-LPTLIQIQGAKDGSIGVNYGTLADNLPPPSKV 87

Query: 80  AAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQ 139
           AAFLLD+TIIDRVRLFDA+PDILRAFAHTGISVSITIPNDQIPRL KPNFAEEWIKFNI+
Sbjct: 88  AAFLLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIE 147

Query: 140 PYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGIL 199
           PYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQ LHTAL E SLDRRIQISTPHSLGIL
Sbjct: 148 PYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGIL 207

Query: 200 SNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGV 259
           SNS+PPST RFRQGYDTHV+KPMLSFLRETNSPLMVNPYPFFACTA+NLDYALFRPNPGV
Sbjct: 208 SNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGV 267

Query: 260 FDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYY 319
           FDSD GILYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGD  Q+GVGPKEAA Y
Sbjct: 268 FDSDSGILYTNMLDAQLDAVYSAMKSLGFSDLDIVIAETGWPSKGDQAQIGVGPKEAADY 327

Query: 320 NGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGIL 379
           NGNLMRHVVSGKGTPLMPNRTFETYIFALFNE+LKPGP+GERNFGLFEPDLSPVYEIGIL
Sbjct: 328 NGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGIL 387

Query: 380 RPTAQSATPRAHQGQVEG---------PSPSTVSESKRWCLPKSEASEEGLQRNIDYVCG 439
           RPTAQS+TP+AHQG +EG         PSPS VSE KRWCLPK+ A  E LQRNIDYVCG
Sbjct: 388 RPTAQSSTPKAHQGPIEGSVSTPRSPSPSPSPVSEGKRWCLPKTGADSEALQRNIDYVCG 447

Query: 440 LGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKC 494
           LGLDCGPI+ENG CFAPNTVRAHAAYVMNAYFQATEGND DCDFDQTG LTTVDPSYGKC
Sbjct: 448 LGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKC 507

BLAST of CSPI05G02940 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 598.2 bits (1541), Expect = 6.0e-171
Identity = 288/461 (62.47%), Postives = 355/461 (77.01%), Query Frame = 0

Query: 33  LLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRV 92
           +LP+FS     +  I+  L  +   +  SIGVNYGT+A+NLPPP +VA FLL +T+I+R+
Sbjct: 4   VLPFFS---LSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRI 63

Query: 93  RLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLV 152
           RLFD DP IL+AFA+TGI+V++T+PNDQIP L   + A++WI  +IQP+ P+TNIIR+LV
Sbjct: 64  RLFDTDPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILV 123

Query: 153 GNEVLSTANKLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILSNSTPPSTARFRQ 212
           GNEV+STA+ LLI  L+PAMQ LHTAL+  SL RRIQISTPHSLG L+NSTPPS+A+FR+
Sbjct: 124 GNEVISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRR 183

Query: 213 GYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDSDLGILYTNML 272
           GYD  V+KP+LSFLR T+SP +VNPYPFF  + + LD+ALFRPNPG+FD    +LYTNML
Sbjct: 184 GYDAQVLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNML 243

Query: 273 DAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKG 332
           DAQLD+VYSAM  LGF D++IVI E GWPS+GD  Q+GV    AA +N NL+  V SG G
Sbjct: 244 DAQLDSVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTG 303

Query: 333 TPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQ 392
           TPLMPNRTFETYIFALFNENLK GP  ERNFG+F  DL+P+Y+IGILRPT +S+ P    
Sbjct: 304 TPLMPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDP---- 363

Query: 393 GQVEGP-SPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVR 452
             V  P SP   S SKRWC+ K+ A    LQRNIDYVCGLGLDC PI E G C+ PNTV+
Sbjct: 364 --VYNPRSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVK 423

Query: 453 AHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 493
           AH+ Y MN Y+Q  E ++FDCDFD TG +TT+DPSYG C+Y
Sbjct: 424 AHSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of CSPI05G02940 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 585.9 bits (1509), Expect = 3.1e-167
Identity = 284/455 (62.42%), Postives = 350/455 (76.92%), Query Frame = 0

Query: 33  LLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRV 92
           +LP+FS     +  I+  L  +   +  SIGVNYGT+A+NLPPP +VA FLL +T+I+R+
Sbjct: 4   VLPFFS---LSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRI 63

Query: 93  RLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLV 152
           RLFD DP IL+AFA+TGI+V++T+PNDQIP L   + A++WI  +IQP+ P+TNIIR+LV
Sbjct: 64  RLFDTDPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILV 123

Query: 153 GNEVLSTANKLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILSNSTPPSTARFRQ 212
           GNEV+STA+ LLI  L+PAMQ LHTAL+  SL RRIQISTPHSLG L+NSTPPS+A+FR+
Sbjct: 124 GNEVISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRR 183

Query: 213 GYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDSDLGILYTNML 272
           GYD  V+KP+LSFLR T+SP +VNPYPFF  + + LD+ALFRPNPG+FD    +LYTNML
Sbjct: 184 GYDAQVLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNML 243

Query: 273 DAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKG 332
           DAQLD+VYSAM  LGF D++IVI E GWPS+GD  Q+GV    AA +N NL+  V SG G
Sbjct: 244 DAQLDSVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTG 303

Query: 333 TPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQ 392
           TPLMPNRTFETYIFALFNENLK GP  ERNFG+F  DL+P+Y+IGILRPT +S+ P    
Sbjct: 304 TPLMPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDP---- 363

Query: 393 GQVEGP-SPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVR 452
             V  P SP   S SKRWC+ K+ A    LQRNIDYVCGLGLDC PI E G C+ PNTV+
Sbjct: 364 --VYNPRSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVK 423

Query: 453 AHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPS 487
           AH+ Y MN Y+Q  E ++FDCDFD TG +TT+DPS
Sbjct: 424 AHSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPS 449

BLAST of CSPI05G02940 vs. TAIR 10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 435.3 bits (1118), Expect = 6.7e-122
Identity = 218/457 (47.70%), Postives = 297/457 (64.99%), Query Frame = 0

Query: 42  TFLLLILPTLFQIQGVNGGS-IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPD 101
           + L L+L  LF I   N  S IGVNYG ++DNLPPPS+ A  LL +T I +VRL++AD  
Sbjct: 4   SILFLLLFILFSISPSNAQSFIGVNYGLLSDNLPPPSQTAK-LLQSTSIQKVRLYNADSS 63

Query: 102 ILRAFAHTGISVSITIPNDQIPRLVKP-NFAEEWIKFNIQPYIPATNIIRVLVGNEVLST 161
           I+ +   TGI + I + N  +P +    N A +WI  N+ P+ PA+NII + VGNEVL +
Sbjct: 64  IITSLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLLS 123

Query: 162 ANKLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVI 221
            +  L+  L+PAMQ +  AL   SL  +I++ST H++ +L NS PPS   F   Y    +
Sbjct: 124 NDLNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAG-L 183

Query: 222 KPMLSFLRETNSPLMVNPYPFFACTAD----NLDYALFRPNPGVFDSDLGILYTNMLDAQ 281
           K +L FL +T SP  +NPYPFFA  +D     L + LF+PNPG  DS+ GI Y NM DAQ
Sbjct: 184 KGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDAQ 243

Query: 282 LDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPL 341
           +DAV+SA+KS+GFE +++++AETGWPS GD  +VG   + A  YNGNL+ H+ S  GTPL
Sbjct: 244 VDAVHSALKSIGFEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTPL 303

Query: 342 MPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQV 401
           MP ++ +TYIFALF+ENLKPGP  E++FGLF+PDLS  Y+IG+ + T+   +     G+V
Sbjct: 304 MPGKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTKTTSSQTSQSPQLGKV 363

Query: 402 EGPSPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAA 461
                     S  WC+PK +A++E LQ ++D+VCG G+DCGPI   G CF PN V +H A
Sbjct: 364 ---------TSMGWCVPKEDATQEQLQDSLDWVCGQGIDCGPIMPGGVCFEPNNVASHTA 423

Query: 462 YVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 493
           Y MN YFQ +  N  DCDF +T  +T+ +PSY  C Y
Sbjct: 424 YAMNLYFQKSPENPTDCDFSKTARITSENPSYSSCVY 449

BLAST of CSPI05G02940 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 428.3 bits (1100), Expect = 8.2e-120
Identity = 215/436 (49.31%), Postives = 286/436 (65.60%), Query Frame = 0

Query: 62  IGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQI 121
           IGVNYG VADNLPPPS+    LL +T I +VRL+ ADP I++A A TG+ + I   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVK-LLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 122 PRLVK-PNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQRLHTALI 181
           P L   PN A +WI  N+ P+ PA+ I+ + VGNE+L + +  L+  L+PAMQ +  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 182 ETSLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPF 241
             SL  +I++ST +S+ +L +S PPS+  F  GY T  +K +L FL +T SP  +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 242 FACTAD----NLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 301
           FA  +D     L + LF PN G  DS  GI YTNM DAQ+DAV+SA+KS+GFE ++IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 302 ETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPG 361
           ETGW S+GD  +VG     A  YNGNL+ H+ S  GTPLMP +  +TYIFAL++ENLKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 362 PIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPSTVSESKRWCLPKSEA 421
           P  ER FGLF+ DLS VY++G+ + ++ S TP    G+V          S  WC+PK  A
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSSQTP---SGKV---------TSSGWCVPKKGA 385

Query: 422 SEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDFDQ 481
           + E LQ ++D+ CG G+DCG I+  GACF PN V +HAAY MN YFQ +     DCDF +
Sbjct: 386 TNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSK 445

Query: 482 TGTLTTVDPSYGKCKY 493
           T T+T+ +PSY  C Y
Sbjct: 446 TATVTSQNPSYNNCVY 447

BLAST of CSPI05G02940 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 406.8 bits (1044), Expect = 2.6e-113
Identity = 211/454 (46.48%), Postives = 294/454 (64.76%), Query Frame = 0

Query: 42  TFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDI 101
           + LLL+   +F        +IGVNYGT+  NLPPP++VA F+   T ID V++FD +PDI
Sbjct: 9   SLLLLLCAAVFLTIPAVISAIGVNYGTLG-NLPPPTQVANFIKTQTSIDSVKIFDVNPDI 68

Query: 102 LRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTAN 161
           LRAFA TGISV +T+PN  IP L     A  W+  NI P+ P T I  + VGNE+L T +
Sbjct: 69  LRAFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVGNEILLTGD 128

Query: 162 KLLIANLVPAMQRLHTALIETSLDRRIQISTPHSLGILSN--STPPSTARFRQGYDTHVI 221
             +I NL+PAM+ L+ AL+   + R ++++T HSL I++   +  PS+ RFR G+D  ++
Sbjct: 129 NNMINNLLPAMRNLNNALVRAGV-RDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWDKGIL 188

Query: 222 KPMLSFLRETNSPLMVNPYPFFACTADNLDYALFR-PNPGVFDSDLGILYTNMLDAQLDA 281
            P+L++ R T SP MVNPYP+F     N+++A+FR P   V D     +YTNM DA +D+
Sbjct: 189 APILAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDALMDS 248

Query: 282 VYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPN 341
            YSAMK+LG+ D++IV+ ETGWPS  D       P  AA++N N+++    G+GTPLMPN
Sbjct: 249 TYSAMKALGYGDVNIVVGETGWPSACDAP--WCSPANAAWFNLNIIKR-AQGQGTPLMPN 308

Query: 342 RTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGP 401
           R FETYIF LFNE  KPGP  ERN+GLF  D SPVY++G+LR        +   G+   P
Sbjct: 309 RRFETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLLR------NGQGGGGRPALP 368

Query: 402 SPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVM 461
           +PST     +WC+ +S A+   LQ +I++VCG G+DC PI+  G+CF P+++R HA++VM
Sbjct: 369 APSTA--GGKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCFNPSSLRTHASFVM 428

Query: 462 NAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 493
           NAYFQ+    D  C+F  TG +   +PS G CKY
Sbjct: 429 NAYFQSHGRTDGACNFSGTGMIVGNNPSNGACKY 449

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P524095.5e-12951.95Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
Q9M0691.2e-11849.31Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94G861.1e-11345.08Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Q8VYE51.4e-9239.34Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9ZU912.3e-9040.31Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KJS04.2e-28999.59(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3... [more]
A0A1S3AV368.2e-26991.80(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV... [more]
A0A5A7TGS63.6e-25692.80(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1H2P32.5e-23384.45(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 P... [more]
A0A6J1K7802.9e-22980.60(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=... [more]
Match NameE-valueIdentityDescription
XP_011654592.18.7e-28999.59glucan endo-1,3-beta-glucosidase isoform X1 [Cucumis sativus][more]
KAE8647796.14.6e-27496.12hypothetical protein Csa_000195 [Cucumis sativus][more]
XP_008437641.11.7e-26891.80PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo][more]
KAA0042530.17.4e-25692.80glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa] >TYK05933.1 glucan e... [more]
XP_038906871.17.7e-24587.58glucan endo-1,3-beta-glucosidase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G24318.16.0e-17162.47O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.23.1e-16762.42O-Glycosyl hydrolases family 17 protein [more]
AT2G16230.16.7e-12247.70O-Glycosyl hydrolases family 17 protein [more]
AT4G34480.18.2e-12049.31O-Glycosyl hydrolases family 17 protein [more]
AT3G55430.12.6e-11346.48O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 60..382
e-value: 1.6E-117
score: 394.0
NoneNo IPR availablePANTHERPTHR32227:SF62O-GLYCOSYL HYDROLASES FAMILY 17 PROTEINcoord: 55..384
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 62..376
e-value: 5.2E-77
score: 259.5
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 291..304
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 55..384
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 62..377

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G02940.1CSPI05G02940.1mRNA
CSPI05G02940.2CSPI05G02940.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds