CSPI05G01090 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G01090
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionULP_PROTEASE domain-containing protein
LocationChr5: 1404371 .. 1404765 (+)
RNA-Seq ExpressionCSPI05G01090
SyntenyCSPI05G01090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCTCAAAGAATAACAAAATAGTTCTTGCTCCTTTTAATCCTAGGTAAGTCAAGTTATTTTATTACTTGCATTGCAAAAAACTAGTACTAATATTTTGTATAATTAGGGGTCATTGGGCACTGCTGGCCATAAATGCATATGATGATATAGTATTTTACCTCGACTCACTAAGGACAACATCGAAGTCAACTATAAGATACGTCACCGACACGTAAGTTAATCTAGTATTTCATTTAGTGTTGATTCTTATATGCATGTACACAATATTTAAACTATTTGTTCAATCTTATCTTGTCAAAACAGAGCAATAGCGATGTTTCAGTCACAAAAGAACATTAAAAAAAGTAGAAAACAAACTTTATGGCGAACAGTAAAGGCAAGTATCAACTTT

mRNA sequence

ATGGTCTCAAAGAATAACAAAATAGTTCTTGCTCCTTTTAATCCTAGGGGTCATTGGGCACTGCTGGCCATAAATGCATATGATGATATAGTATTTTACCTCGACTCACTAAGGACAACATCGAAGTCAACTATAAGATACGTCACCGACACAGCAATAGCGATGTTTCAGTCACAAAAGAACATTAAAAAAAGTAGAAAACAAACTTTATGGCGAACAGTAAAGGCAAGTATCAACTTT

Coding sequence (CDS)

ATGGTCTCAAAGAATAACAAAATAGTTCTTGCTCCTTTTAATCCTAGGGGTCATTGGGCACTGCTGGCCATAAATGCATATGATGATATAGTATTTTACCTCGACTCACTAAGGACAACATCGAAGTCAACTATAAGATACGTCACCGACACAGCAATAGCGATGTTTCAGTCACAAAAGAACATTAAAAAAAGTAGAAAACAAACTTTATGGCGAACAGTAAAGGCAAGTATCAACTTT

Protein sequence

MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQKNIKKSRKQTLWRTVKASINF
Homology
BLAST of CSPI05G01090 vs. ExPASy TrEMBL
Match: A0A5A7SP28 (ULP_PROTEASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold32G00850 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.5e-26
Identity = 61/73 (83.56%), Postives = 67/73 (91.78%), Query Frame = 0

Query: 1   MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60
           MVSK +++VLAPFNP GHWALLAINAY+D VFYLDSLRTTSK+T RYVTDTAIA+F SQK
Sbjct: 116 MVSKPDQVVLAPFNPGGHWALLAINAYEDTVFYLDSLRTTSKATTRYVTDTAIAIFHSQK 175

Query: 61  NIKKSRKQTLWRT 74
           NI KSRKQTLWRT
Sbjct: 176 NINKSRKQTLWRT 188

BLAST of CSPI05G01090 vs. ExPASy TrEMBL
Match: A0A5D3DME5 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G00220 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.5e-26
Identity = 61/73 (83.56%), Postives = 67/73 (91.78%), Query Frame = 0

Query: 1   MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60
           MVSK +++VLAPFNP GHWALLAINAY+D VFYLDSLRTTSK+T RYVTDTAIA+F SQK
Sbjct: 461 MVSKPDQVVLAPFNPGGHWALLAINAYEDTVFYLDSLRTTSKATTRYVTDTAIAIFHSQK 520

Query: 61  NIKKSRKQTLWRT 74
           NI KSRKQTLWRT
Sbjct: 521 NINKSRKQTLWRT 533

BLAST of CSPI05G01090 vs. ExPASy TrEMBL
Match: A0A5D3C8G6 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G001550 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.5e-26
Identity = 61/73 (83.56%), Postives = 67/73 (91.78%), Query Frame = 0

Query: 1   MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60
           MVSK +++VLAPFNP GHWALLAINAY+D VFYLDSLRTTSK+T RYVTDTAIA+F SQK
Sbjct: 463 MVSKPDQVVLAPFNPGGHWALLAINAYEDTVFYLDSLRTTSKATTRYVTDTAIAIFHSQK 522

Query: 61  NIKKSRKQTLWRT 74
           NI KSRKQTLWRT
Sbjct: 523 NINKSRKQTLWRT 535

BLAST of CSPI05G01090 vs. ExPASy TrEMBL
Match: A0A5D3C8G8 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1737G00550 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.5e-26
Identity = 61/73 (83.56%), Postives = 67/73 (91.78%), Query Frame = 0

Query: 1   MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60
           MVSK +++VLAPFNP GHWALLAINAY+D VFYLDSLRTTSK+T RYVTDTAIA+F SQK
Sbjct: 461 MVSKPDQVVLAPFNPGGHWALLAINAYEDTVFYLDSLRTTSKATTRYVTDTAIAIFHSQK 520

Query: 61  NIKKSRKQTLWRT 74
           NI KSRKQTLWRT
Sbjct: 521 NINKSRKQTLWRT 533

BLAST of CSPI05G01090 vs. ExPASy TrEMBL
Match: A0A5A7U3B1 (Transposase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold76G00930 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.5e-26
Identity = 61/73 (83.56%), Postives = 67/73 (91.78%), Query Frame = 0

Query: 1   MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60
           MVSK +++VLAPFNP GHWALLAINAY+D VFYLDSLRTTSK+T RYVTDTAIA+F SQK
Sbjct: 413 MVSKPDQVVLAPFNPGGHWALLAINAYEDTVFYLDSLRTTSKATTRYVTDTAIAIFHSQK 472

Query: 61  NIKKSRKQTLWRT 74
           NI KSRKQTLWRT
Sbjct: 473 NINKSRKQTLWRT 485

BLAST of CSPI05G01090 vs. NCBI nr
Match: XP_031742076.1 (uncharacterized protein LOC116404043 [Cucumis sativus])

HSP 1 Score: 154.8 bits (390), Expect = 3.1e-34
Identity = 77/80 (96.25%), Postives = 78/80 (97.50%), Query Frame = 0

Query: 1   MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60
           MVSK N+IVLAPFNPRGHWALLAINAYDD VFYLDSLRTTSKSTIRYVTDTAIAMFQSQK
Sbjct: 118 MVSKINEIVLAPFNPRGHWALLAINAYDDTVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 177

Query: 61  NIKKSRKQTLWRTVKASINF 81
           NIKKSRKQTLWRTVKASINF
Sbjct: 178 NIKKSRKQTLWRTVKASINF 197

BLAST of CSPI05G01090 vs. NCBI nr
Match: KAE8647004.1 (hypothetical protein Csa_016998 [Cucumis sativus])

HSP 1 Score: 148.3 bits (373), Expect = 2.9e-32
Identity = 73/78 (93.59%), Postives = 74/78 (94.87%), Query Frame = 0

Query: 1  MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60
          MVSKNNKIVLAPFNP GHWALLAINAYDD VFYLDSLRTTSKSTIRYVTDTAIAMFQSQK
Sbjct: 1  MVSKNNKIVLAPFNPGGHWALLAINAYDDTVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60

Query: 61 NIKKSRKQTLWRTVKASI 79
          NIKKSRKQTLWRTVK  +
Sbjct: 61 NIKKSRKQTLWRTVKCPL 78

BLAST of CSPI05G01090 vs. NCBI nr
Match: KAE8637109.1 (hypothetical protein CSA_009453, partial [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 1.6e-30
Identity = 71/78 (91.03%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 1   MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60
           MVSK N+IVLAPFNP GHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK
Sbjct: 89  MVSKINEIVLAPFNPGGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 148

Query: 61  NIKKSRKQTLWRTVKASI 79
           NIKKSRKQTLW TVK  +
Sbjct: 149 NIKKSRKQTLWLTVKCPL 166

BLAST of CSPI05G01090 vs. NCBI nr
Match: XP_031745418.1 (uncharacterized protein LOC116405627 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 1.6e-30
Identity = 71/78 (91.03%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 1   MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60
           MVSK N+IVLAPFNP GHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK
Sbjct: 159 MVSKINEIVLAPFNPGGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 218

Query: 61  NIKKSRKQTLWRTVKASI 79
           NIKKSRKQTLW TVK  +
Sbjct: 219 NIKKSRKQTLWLTVKCPL 236

BLAST of CSPI05G01090 vs. NCBI nr
Match: XP_031742112.1 (uncharacterized protein LOC116404063 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 1.6e-30
Identity = 71/78 (91.03%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 1  MVSKNNKIVLAPFNPRGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60
          MVSK N+IVLAPFNP GHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK
Sbjct: 1  MVSKINEIVLAPFNPGGHWALLAINAYDDIVFYLDSLRTTSKSTIRYVTDTAIAMFQSQK 60

Query: 61 NIKKSRKQTLWRTVKASI 79
          NIKKSRKQTLW TVK  +
Sbjct: 61 NIKKSRKQTLWLTVKCPL 78

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7SP282.5e-2683.56ULP_PROTEASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A5D3DME52.5e-2683.56Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3C8G62.5e-2683.56Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3C8G82.5e-2683.56Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7U3B12.5e-2683.56Transposase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold76G00930 PE=... [more]
Match NameE-valueIdentityDescription
XP_031742076.13.1e-3496.25uncharacterized protein LOC116404043 [Cucumis sativus][more]
KAE8647004.12.9e-3293.59hypothetical protein Csa_016998 [Cucumis sativus][more]
KAE8637109.11.6e-3091.03hypothetical protein CSA_009453, partial [Cucumis sativus][more]
XP_031745418.11.6e-3091.03uncharacterized protein LOC116405627 [Cucumis sativus][more]
XP_031742112.11.6e-3091.03uncharacterized protein LOC116404063 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 4..63
e-value: 1.2E-7
score: 31.9
NoneNo IPR availableGENE3D3.40.395.10Adenoviral Proteinase; Chain Acoord: 3..78
e-value: 3.0E-7
score: 32.0
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 5..62

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G01090.1CSPI05G01090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0016301 kinase activity