CSPI04G27590 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G27590
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRNA-binding (RRM/RBD/RNP motifs) family protein
LocationChr4: 24199083 .. 24202787 (+)
RNA-Seq ExpressionCSPI04G27590
SyntenyCSPI04G27590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACATTTGTGAGTCAGATTGGACGAGTAATAAAAAGAAAAGCTAAGCATCAAAATCACCCGCCGTGCATTAATCCTCGAGTTCATAGTTATTTAGTTCCTTCTCCAAATCCTCAAATGGAGTTGCCAAGATTCAGCCGTCCAAACCAAGATTATGGATCTTCTTCTTCTTCTACCCCTATTCTCTACGTTGCTAACTGCGGACCCGCCGTCGGAATCAGCCACCCCGCAATCGCGGCCGTTTTTGCCCATTTTGGCCATGTCAAAGGTGTTCACCCCGCCGACGATACCGGCGCTCGTGTCATCGTATGTTTCTCTGAAGAATCCAGCGCCCGAGCCGCTCTTGAGGCGCTTCACGGCCGCCCTTGCCCTCTCCTTGGAGGCCGGACTTTGCACATACGTTATTCCATCACCAGACCATCCATTTCGCAACCTAATGATTCTCTTTCAGTTTCTTTGTCGGCTTCGGAGCTGGACATTCCCGGACTTTTCTTATTGCACGATTTCGTTAATGCTAAAGAAGAGGAGGTGAGCTTTTTAGTTGGATTATGAGGACTAGAGAAGGGTTTTTTCTTTTTCACTCCCTTTGTTCCATCGTTATTGATTTTTCGTGGTTTTGAAGGATTTGCTTAGGGAAGTTGATGCTCGACCTTGGAATAATCTGGCGAAACGCAGAGTTCAGCATTATGGGTATGAGTTTTGTTATCAAGTAAGTGGCAGGACTTTGTCTTTTCCCTCTTCCTACTTGCTGTTGTACTGAATGATTGTGCATTGTTAGGATTTGCGAGTAATGTAAATCAGTTGGATTTGTCTGTGTCTGTTTTTTTCTTTCTAGACGAGGAATGTTAATACTAAACATCAGTTGGGGGAACTTCCATCATTTGTTTCCCATGTAGTTGATAGGATCTCCATGTTTCCAAACACTGAGGATATTGCAGATGCTTCTCTTGATCAATTGACGGTAGGCTCTCTGTTTTTGATGAATCTCTAAACTTTGTTCCTTGTAGTCGGTTAGTTTTTTGTACAATCTTTGAAAATTGAGATTGTAGCTTTTGCTATCTGGAGTTGTTGTGATGTTTAGTATGAGACTGAAATTACTACAACATTACAGGTTAATGAATACCCACCTGGGGTGGGTTTGTCCCCTCATATAGACACCCATTCTGCATTTGAAGGATTAATTTTCAGCCTTTCCTTAGCAGGGCCATGCATTATGGAGTTTAGGAGATATCCTGAAGGCACTTGGCACAAATTCCCTTCAAGTATAGATTTGAAAATGGAGAATTCTGTAAACGACTCAAATTATCTGAGGAAAGCCATTTACCTCCCCCCTCGGTCTATGCTTCTACTGTCTGGAGAGGCACGCTATGTTTGGCATCACTACATTCCTCACCATAAGGTATAGCAAACCATACATGAACAAATTGTTGCTTTAGTTTGGTTGATTTATGAAATTGTAGAGTTATCTATTATACATTTTGATAGGCCCGGTCATACATGTGTTTGCTCGCGAGTAAGGAAAGAGACGATGGTGTGAGAAATTGTAAGGGGTGTGTGGAGCTCATTAGTATGGAGTTCAGTTAGATTAGTATAGTGAGAGGAGGGTATCTTTAGGGAGAACTTTTGTGGCATTCTCTTTGAAAATTAGGAGTGGTTGGAAGCTTTCAGATCTTCTTTGTCATCTTGCTACTGGTTTATAGATAAATTGGCCTAGCCCCAGGCCCCTATCACATGTTAACAGATACGAATTGGTCTAGTAGTCTATTTTCAAATGGTTGGACCAATGGGTAAGGATGAGCTATACATTTGGATGTCTGTAAAAAATTTCAATAGTCATTTACTTATGATAATCATATTCACAAAGTCATTTTTATTTCAAATGTTTTAGAGGATTAAATTATGTTCTTGTTGGATAGCACAAGTGAGGGTAAATTGGACTAGAAACCATGGGTATAAACATTTTTTTGGGTGTTCACAAAGGTGCCAGCCAAGCATACACAGCGGTTAAGAAGATAATTAGTTGTTGTCTGTTGATTAACATTATGATTTTGAACGAGAGACACTGCACTTATATGTTCAACGATATACTTGATAAATAATTTTTACTCATTCTGTTCTCATTTATGATGCTGAAATTGGGCAAATGTTCTGTAATAGATTGACATGGTAAAGGACAGTTCTATCAGAAGGGGTCGTAGGAGAGTTTCTTTTACATTTCGCAAGGTGAGTAGATATAATGCATATGTTCTTTCCCTCCCTCTCGTACCTGTTGAGCTTACCTTACGTGCTTCTATTATCATTGATGTCAAGACAGCTATTATCGTACCCATGGCAATTCTGAAATATTATAAATGTTAAACTTCGGTATCACCAGACCATGTTATTCTAGTTTGATCCATTAATATTGAGGCACCTTGGCAATGGCTATATAACAGTAAAGAGAGGAAGCTAGAATTTAAAATGTCAGCTCTCCTATGGTTTCTTTCTTCCTTCATTTGATTTTGAATGATTGCTCACCTCTTAAAGTTCAGCGTATTTTCTCTTTTAGTGAACTTTAGTGCACAATCCATGGTGGTCACCTACTTAGGATGTAATATCCTAAGTTTTTTTAACACTACACCCAAATATTGTAAGCTCAGAGGAGTTGTCCTGTGAGATCAGCAGAGATGTTTGTAAATTAGCTCGAACAATCGCAAATAAAAAAATAAAAAAATAGCCCAATCTCCTCCACGATTAACTTTTACTCGATCTGTAGATTTTAGTCCTCAAGAGGTGCTTGGCTCATGGGGGCTTTTGGAGCTCATTTGATAGAATATAGGCCACGAAACCACAAATCAATCACAGCCACAAAGAGTACATAAACAGAAATTTGAAACCTTATTCTTCTCAGCATAATTCATATTGAGGTTATTCAAATCAAGAAACCTTTTACATGAAGCACGGTTTTTGCTTTACTAATGCTTGTTTGGCCATCCCGCTTTACAATGATGAAAACTTTTTGATAATAATGGAACTAAATATGTGGAAACTGGTTGAAATGTATGTGAATGGAATGACTAAGCCATGCTACAAAGTTGAAAATCTCAAGCCAATGCCTTGCGACTTGAAGATATGGTGGTAAAAACATATCTAGTAACCAGTTATTCGTTTGCTTCGATTTTCTCGGGATGCTATGCAAAATTCTACCGTTCTCTATGGTCTAAAAGTTGATGTTTTTATTTCATCTTATCATCTCACATGCCTTACCATGGTTGAATAAAGGGGAAAAAAATTGTCTTATTGTAGGTGAGAACTGATCCTTGCCAATGCAAATTTCCCCATTATTGCGATTCTCAGAGATAAATGAAAGGGCTCAATCCTCATTGAAGAACAGGCTGAGTTTTCTTTTTATCCTCATTCATCCATCCTTTTACATATGGTTCTTTAAAGTAGATTTTCTACATATTTTTACTTGGTTATACTTACAGGGGGATTTTTCGATACCTCTCACATTTATTGTTTAAATATTTTACCCTCTTATGTTCTAATTATGGATGCAGCAACAGCATTTTGTTAAACTCCCATTAATTACAGGGAATGAAACTGGGGATGTGGAAACGTTATAGAGGTTAAAATTCAGGACCTCTTTTCTCACTTTTATTCATTTTAAAATCGTTTTAATTGTCTGTTTGATAATGTTATTTTGGCGTAAATGATTGTTTGATAAC

mRNA sequence

CACATTTGTGAGTCAGATTGGACGAGTAATAAAAAGAAAAGCTAAGCATCAAAATCACCCGCCGTGCATTAATCCTCGAGTTCATAGTTATTTAGTTCCTTCTCCAAATCCTCAAATGGAGTTGCCAAGATTCAGCCGTCCAAACCAAGATTATGGATCTTCTTCTTCTTCTACCCCTATTCTCTACGTTGCTAACTGCGGACCCGCCGTCGGAATCAGCCACCCCGCAATCGCGGCCGTTTTTGCCCATTTTGGCCATGTCAAAGGTGTTCACCCCGCCGACGATACCGGCGCTCGTGTCATCGTATGTTTCTCTGAAGAATCCAGCGCCCGAGCCGCTCTTGAGGCGCTTCACGGCCGCCCTTGCCCTCTCCTTGGAGGCCGGACTTTGCACATACGTTATTCCATCACCAGACCATCCATTTCGCAACCTAATGATTCTCTTTCAGTTTCTTTGTCGGCTTCGGAGCTGGACATTCCCGGACTTTTCTTATTGCACGATTTCGTTAATGCTAAAGAAGAGGAGGATTTGCTTAGGGAAGTTGATGCTCGACCTTGGAATAATCTGGCGAAACGCAGAGTTCAGCATTATGGGTATGAGTTTTGTTATCAAACGAGGAATGTTAATACTAAACATCAGTTGGGGGAACTTCCATCATTTGTTTCCCATGTAGTTGATAGGATCTCCATGTTTCCAAACACTGAGGATATTGCAGATGCTTCTCTTGATCAATTGACGGTTAATGAATACCCACCTGGGGTGGGTTTGTCCCCTCATATAGACACCCATTCTGCATTTGAAGGATTAATTTTCAGCCTTTCCTTAGCAGGGCCATGCATTATGGAGTTTAGGAGATATCCTGAAGGCACTTGGCACAAATTCCCTTCAAGTATAGATTTGAAAATGGAGAATTCTGTAAACGACTCAAATTATCTGAGGAAAGCCATTTACCTCCCCCCTCGGTCTATGCTTCTACTGTCTGGAGAGGCACGCTATGTTTGGCATCACTACATTCCTCACCATAAGATTGACATGGTAAAGGACAGTTCTATCAGAAGGGGTCGTAGGAGAGTTTCTTTTACATTTCGCAAGGTGAGAACTGATCCTTGCCAATGCAAATTTCCCCATTATTGCGATTCTCAGAGATAAATGAAAGGGCTCAATCCTCATTGAAGAACAGGCTGAGTTTTCTTTTTATCCTCATTCATCCATCCTTTTACATATGGTTCTTTAAAGTAGATTTTCTACATATTTTTACTTGGTTATACTTACAGGGGGATTTTTCGATACCTCTCACATTTATTGTTTAAATATTTTACCCTCTTATGTTCTAATTATGGATGCAGCAACAGCATTTTGTTAAACTCCCATTAATTACAGGGAATGAAACTGGGGATGTGGAAACGTTATAGAGGTTAAAATTCAGGACCTCTTTTCTCACTTTTATTCATTTTAAAATCGTTTTAATTGTCTGTTTGATAATGTTATTTTGGCGTAAATGATTGTTTGATAAC

Coding sequence (CDS)

ATGGAGTTGCCAAGATTCAGCCGTCCAAACCAAGATTATGGATCTTCTTCTTCTTCTACCCCTATTCTCTACGTTGCTAACTGCGGACCCGCCGTCGGAATCAGCCACCCCGCAATCGCGGCCGTTTTTGCCCATTTTGGCCATGTCAAAGGTGTTCACCCCGCCGACGATACCGGCGCTCGTGTCATCGTATGTTTCTCTGAAGAATCCAGCGCCCGAGCCGCTCTTGAGGCGCTTCACGGCCGCCCTTGCCCTCTCCTTGGAGGCCGGACTTTGCACATACGTTATTCCATCACCAGACCATCCATTTCGCAACCTAATGATTCTCTTTCAGTTTCTTTGTCGGCTTCGGAGCTGGACATTCCCGGACTTTTCTTATTGCACGATTTCGTTAATGCTAAAGAAGAGGAGGATTTGCTTAGGGAAGTTGATGCTCGACCTTGGAATAATCTGGCGAAACGCAGAGTTCAGCATTATGGGTATGAGTTTTGTTATCAAACGAGGAATGTTAATACTAAACATCAGTTGGGGGAACTTCCATCATTTGTTTCCCATGTAGTTGATAGGATCTCCATGTTTCCAAACACTGAGGATATTGCAGATGCTTCTCTTGATCAATTGACGGTTAATGAATACCCACCTGGGGTGGGTTTGTCCCCTCATATAGACACCCATTCTGCATTTGAAGGATTAATTTTCAGCCTTTCCTTAGCAGGGCCATGCATTATGGAGTTTAGGAGATATCCTGAAGGCACTTGGCACAAATTCCCTTCAAGTATAGATTTGAAAATGGAGAATTCTGTAAACGACTCAAATTATCTGAGGAAAGCCATTTACCTCCCCCCTCGGTCTATGCTTCTACTGTCTGGAGAGGCACGCTATGTTTGGCATCACTACATTCCTCACCATAAGATTGACATGGTAAAGGACAGTTCTATCAGAAGGGGTCGTAGGAGAGTTTCTTTTACATTTCGCAAGGTGAGAACTGATCCTTGCCAATGCAAATTTCCCCATTATTGCGATTCTCAGAGATAA

Protein sequence

MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGARVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELDIPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELPSFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGPCIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYIPHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR*
Homology
BLAST of CSPI04G27590 vs. ExPASy Swiss-Prot
Match: Q8RWY1 (Alkylated DNA repair protein ALKBH8 homolog OS=Arabidopsis thaliana OX=3702 GN=ALKBH8 PE=2 SV=2)

HSP 1 Score: 452.2 bits (1162), Expect = 5.2e-126
Identity = 234/349 (67.05%), Postives = 266/349 (76.22%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTP---ILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADD 60
           M  PRF RP Q   SS S  P    LYVANCGPAVG++H AIAAVFA FG V GV+ ADD
Sbjct: 1   MVQPRFVRPTQSSPSSISGEPNSSNLYVANCGPAVGLTHNAIAAVFAEFGEVNGVYAADD 60

Query: 61  TGARVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITR-PSISQPNDSLSVSLSA 120
           +G RVIV F++  SA+AALEAL GRPCP L GR+LHIRYS+ + PS +Q ND + VSL  
Sbjct: 61  SGVRVIVSFADPFSAKAALEALSGRPCPDLKGRSLHIRYSVLQLPSETQVNDCVPVSLID 120

Query: 121 SELDIPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQL 180
           SEL+IPGLFLL DFV   EE+ LL  VDAR W  LAKRRVQHYGYEFCY TRNV+TK +L
Sbjct: 121 SELNIPGLFLLPDFVTVAEEQQLLAAVDARHWIGLAKRRVQHYGYEFCYGTRNVDTKKRL 180

Query: 181 GELPSFVSHVVDRISMFPNTED-IADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSL 240
           GELPSFVS +++RI +FPN ++  A  +LDQLTVNEYP GVGLSPHIDTHSAFE  IFSL
Sbjct: 181 GELPSFVSPILERIYLFPNFDNGSASLNLDQLTVNEYPSGVGLSPHIDTHSAFEDCIFSL 240

Query: 241 SLAGPCIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYV 300
           SLAGPCIMEFRRY   TW    S+ D +      DS+ ++KA+YLPPRSMLLLSGEARY 
Sbjct: 241 SLAGPCIMEFRRYSVSTWK--ASTTDAEKS---GDSSCIKKALYLPPRSMLLLSGEARYA 300

Query: 301 WHHYIPHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           W+HYIPHHKID VKD  IRR  RRVSFT RKVR  PC CK+P YCDSQ+
Sbjct: 301 WNHYIPHHKIDKVKDKVIRRSSRRVSFTLRKVRNHPCSCKYPQYCDSQQ 344

BLAST of CSPI04G27590 vs. ExPASy Swiss-Prot
Match: Q07G10 (Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alkbh8 PE=2 SV=2)

HSP 1 Score: 154.5 bits (389), Expect = 2.3e-36
Identity = 112/344 (32.56%), Postives = 163/344 (47.38%), Query Frame = 0

Query: 23  LYVANCGPAVGISHPAIAAVFAHFGHVKG-VHPADDTGARVIVCFSEESSARAALEALHG 82
           L VAN G   G+S   + AV    G V+  + P +   A   V +S    A  A  +L G
Sbjct: 45  LVVANGGLGNGVSRQQLLAVLERCGKVETLLMPPNKPYA--FVTYSSAEEAIKAYSSLSG 104

Query: 83  RP-CPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELDIPGLFLLHDFVNAKEEEDLL 142
           +  C     + + +  S     + +  + LS SL       PGL ++ DFV+ ++E  +L
Sbjct: 105 QELCGEDAEQPITLYLSFVEKVVVK--EVLSPSLP------PGLIIVEDFVSPEQERTML 164

Query: 143 REVD----ARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQL-GELPSFVSHVVDRISMFPN 202
             +D         +L  R+V+HYGYEF Y   NV+    L G LP F +  + +      
Sbjct: 165 ESIDWDSETSSQKSLKHRQVKHYGYEFRYDNNNVDKDKPLPGGLPDFCTEALRKCVQ--- 224

Query: 203 TEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGPCIMEFRRYPEGTWHK 262
              +     DQLT+N+Y PG G+ PH+DTHSAFE  I SLSL    +M+F ++P G+   
Sbjct: 225 -RGLIKHDPDQLTINQYEPGQGIPPHVDTHSAFEDEILSLSLGAEIVMDF-KHPNGS--- 284

Query: 263 FPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYIPHHKIDMVKDS---- 322
                                 + LP RS+L++SGE+RY+W H I   K D+++ S    
Sbjct: 285 -------------------VVPVMLPQRSLLIMSGESRYLWTHGITPRKFDVIQVSEGQT 344

Query: 323 -----------SIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
                      ++ +   R SFTFRKVR  PC C FP  CDSQ+
Sbjct: 345 VGTISGNSGELTLSKRSTRTSFTFRKVRHSPCDCAFPSECDSQQ 351

BLAST of CSPI04G27590 vs. ExPASy Swiss-Prot
Match: Q95K79 (Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.0e-33
Identity = 87/246 (35.37%), Postives = 124/246 (50.41%), Query Frame = 0

Query: 122 PGLFLLHDFVNAKEEEDLLREVD-------ARPWNNLAKRRVQHYGYEFCYQTRNVNTKH 181
           PGL ++ + ++++EE+ LL  VD            +L  RRV+H+GYEF Y+  NV+   
Sbjct: 135 PGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFGYEFHYENNNVDKDK 194

Query: 182 QL-GELPSFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIF 241
            L G LP      +++       E       DQ+T+N+Y PG G+  HIDTHSAFE  I 
Sbjct: 195 PLPGGLPDICDSFLEKWL----REGYIKHKPDQMTINQYEPGQGIPAHIDTHSAFEDEIV 254

Query: 242 SLSLAGPCIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEAR 301
           SLSL    +M+F ++P+GT                         + LP RS+L+++GE+R
Sbjct: 255 SLSLGSEIVMDF-KHPDGT----------------------AVPVMLPRRSLLVMTGESR 314

Query: 302 YVWHHYIPHHKIDMVK---------------DSSIRRGRRRVSFTFRKVRTDPCQCKFPH 345
           Y+W H I   K D V+               D ++ +   R SFTFRKVR  PC C +P 
Sbjct: 315 YLWTHGITCRKFDTVQASENHKSGIITSDVGDLTLSKRGLRTSFTFRKVRQTPCNCSYPL 353

BLAST of CSPI04G27590 vs. ExPASy Swiss-Prot
Match: Q96BT7 (Alkylated DNA repair protein alkB homolog 8 OS=Homo sapiens OX=9606 GN=ALKBH8 PE=1 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 2.6e-32
Identity = 85/246 (34.55%), Postives = 124/246 (50.41%), Query Frame = 0

Query: 122 PGLFLLHDFVNAKEEEDLLREVD-------ARPWNNLAKRRVQHYGYEFCYQTRNVNTKH 181
           PGL ++ + ++++EE+ LL  VD            +L  RRV+H+GYEF Y+  NV+   
Sbjct: 135 PGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFGYEFHYENNNVDKDK 194

Query: 182 QL-GELPSFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIF 241
            L G LP      +++       +       DQ+T+N+Y PG G+  HIDTHSAFE  I 
Sbjct: 195 PLSGGLPDICESFLEKWL----RKGYIKHKPDQMTINQYEPGQGIPAHIDTHSAFEDEIV 254

Query: 242 SLSLAGPCIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEAR 301
           SLSL    +M+F ++P+G                      +   + LP RS+L+++GE+R
Sbjct: 255 SLSLGSEIVMDF-KHPDG----------------------IAVPVMLPRRSLLVMTGESR 314

Query: 302 YVWHHYIPHHKIDMVK---------------DSSIRRGRRRVSFTFRKVRTDPCQCKFPH 345
           Y+W H I   K D V+               D ++ +   R SFTFRKVR  PC C +P 
Sbjct: 315 YLWTHGITCRKFDTVQASESLKSGIITSDVGDLTLSKRGLRTSFTFRKVRQTPCNCSYPL 353

BLAST of CSPI04G27590 vs. ExPASy Swiss-Prot
Match: Q80Y20 (Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.4e-32
Identity = 86/249 (34.54%), Postives = 126/249 (50.60%), Query Frame = 0

Query: 122 PGLFLLHDFVNAKEEEDLLREVDARPW----------NNLAKRRVQHYGYEFCYQTRNVN 181
           PGL ++ + ++++EE+ LL  V+   W           +L  RRV+H+GYEF Y++  V+
Sbjct: 135 PGLLVVEEIISSEEEKKLLESVN---WTEDTGNQNFQRSLKHRRVKHFGYEFHYESNTVD 194

Query: 182 TKHQL-GELPSFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEG 241
               L G LP   S +++++      E       DQLT+N+Y PG G+  HIDTHSAFE 
Sbjct: 195 KDKPLPGGLPEVCSSILEKLL----KEGYIKHKPDQLTINQYEPGHGIPAHIDTHSAFED 254

Query: 242 LIFSLSLAGPCIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSG 301
            I SLSL    +M+F ++PEG                      +   + LP RS+L+++G
Sbjct: 255 EIISLSLGSAIVMDF-KHPEG----------------------VTVQVMLPRRSLLVMTG 314

Query: 302 EARYVWHHYIPHHKIDMVK---------------DSSIRRGRRRVSFTFRKVRTDPCQCK 345
           E+RY+W H I   K D V+               D ++ +   R SFTFRKVR  PC C 
Sbjct: 315 ESRYLWTHGITPRKFDTVQASEQFKGGIITSDIGDLTLSKRGMRTSFTFRKVRRMPCNCS 353

BLAST of CSPI04G27590 vs. ExPASy TrEMBL
Match: A0A0A0L3H6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G669210 PE=3 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 4.2e-203
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60
           MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA
Sbjct: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60

Query: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120
           RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD
Sbjct: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120

Query: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180
           IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP
Sbjct: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180

Query: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240
           SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP
Sbjct: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240

Query: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300
           CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI
Sbjct: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300

Query: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR
Sbjct: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 344

BLAST of CSPI04G27590 vs. ExPASy TrEMBL
Match: A0A1S3B9H0 (alkylated DNA repair protein alkB homolog 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487636 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.1e-190
Identity = 324/344 (94.19%), Postives = 330/344 (95.93%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60
           MELPRF+RPNQ  GSSSS  PILYVANCGPAVGISHP IAAVFAHFGHVKGVH ADDTGA
Sbjct: 1   MELPRFTRPNQHNGSSSS--PILYVANCGPAVGISHPTIAAVFAHFGHVKGVHAADDTGA 60

Query: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120
           RVIVCFSEESSA+AALE LHGRPCPLLGGRTLHIRYSI RPSISQPNDS+SVSLSASELD
Sbjct: 61  RVIVCFSEESSAQAALETLHGRPCPLLGGRTLHIRYSIIRPSISQPNDSVSVSLSASELD 120

Query: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180
           IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP
Sbjct: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180

Query: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240
            FVSHVVDRIS+FPNTED+ADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP
Sbjct: 181 PFVSHVVDRISLFPNTEDVADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240

Query: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300
           CIMEFRRYPEG WH+FP SIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARY WHHYI
Sbjct: 241 CIMEFRRYPEGAWHEFPLSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYAWHHYI 300

Query: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           PHHKIDMVKDSSIRR  RRVSFTFRKVRTDPCQCKFPHYCDSQR
Sbjct: 301 PHHKIDMVKDSSIRRAPRRVSFTFRKVRTDPCQCKFPHYCDSQR 342

BLAST of CSPI04G27590 vs. ExPASy TrEMBL
Match: A0A6J1FEN4 (alkylated DNA repair protein alkB homolog 8 OS=Cucurbita moschata OX=3662 GN=LOC111443258 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 2.9e-180
Identity = 310/344 (90.12%), Postives = 322/344 (93.60%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60
           M+LPRF+RP  D GSSSSS P LYVANCGPAVGISH A+AAVF  FG VKGVH AD+TG 
Sbjct: 1   MDLPRFARPRGDNGSSSSS-PNLYVANCGPAVGISHRAVAAVFGDFGDVKGVHAADETGT 60

Query: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120
           RVIVCFSEESSARAALEALHGRPC LLGGRTLHIRYSI RPSISQ NDS+SVSLSASELD
Sbjct: 61  RVIVCFSEESSARAALEALHGRPCSLLGGRTLHIRYSIIRPSISQLNDSISVSLSASELD 120

Query: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180
           IPGL+LLHDFV AKEEE+LL EVDARPWNNLAKRRVQHYGYEFCYQTRNVNT+HQLGELP
Sbjct: 121 IPGLYLLHDFVTAKEEEELLSEVDARPWNNLAKRRVQHYGYEFCYQTRNVNTRHQLGELP 180

Query: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240
           SFVSHVVDRISMFPN ED+ADA LDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP
Sbjct: 181 SFVSHVVDRISMFPNAEDVADAVLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240

Query: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300
           CIMEFRRY EGTWHK PSSIDLKMENSVNDSNYLR+AIYLPPRSMLLLSGEARY WHHYI
Sbjct: 241 CIMEFRRYTEGTWHKCPSSIDLKMENSVNDSNYLRRAIYLPPRSMLLLSGEARYAWHHYI 300

Query: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           PHHKIDMVKDS+IRRG RRVSFTFRKVRTDPC+CKFPHYCDSQR
Sbjct: 301 PHHKIDMVKDSAIRRGPRRVSFTFRKVRTDPCRCKFPHYCDSQR 343

BLAST of CSPI04G27590 vs. ExPASy TrEMBL
Match: A0A6J1IPP9 (alkylated DNA repair protein alkB homolog 8 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477075 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.1e-178
Identity = 307/344 (89.24%), Postives = 320/344 (93.02%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60
           M+LPRF+RP  + GSSSSS P LYVANCGPAVGISH  +AAVF  FG VKGVH AD+TG 
Sbjct: 1   MDLPRFARPRGENGSSSSS-PNLYVANCGPAVGISHRTVAAVFGDFGDVKGVHAADETGT 60

Query: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120
           RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSI RPSISQ NDS+SVSLSASEL+
Sbjct: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSIIRPSISQLNDSVSVSLSASELN 120

Query: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180
           IPGL+LLHDFV AKEEE+LL EVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLG LP
Sbjct: 121 IPGLYLLHDFVTAKEEEELLLEVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGGLP 180

Query: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240
           SFVSHVVDRISMFPN ED+ADA LDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP
Sbjct: 181 SFVSHVVDRISMFPNAEDVADAVLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240

Query: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300
           CIMEFRRY EGTWHK PSSIDLKMENSVNDSNYLR+AIYLPPRSMLLLSGEARY WHHYI
Sbjct: 241 CIMEFRRYTEGTWHKCPSSIDLKMENSVNDSNYLRRAIYLPPRSMLLLSGEARYAWHHYI 300

Query: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           PHHKIDMVKDS+IRRG RRVSFTFRKVRTDPC+CKFPHYCDS R
Sbjct: 301 PHHKIDMVKDSAIRRGPRRVSFTFRKVRTDPCRCKFPHYCDSHR 343

BLAST of CSPI04G27590 vs. ExPASy TrEMBL
Match: A0A5D3BS48 (Alkylated DNA repair protein alkB-like protein 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G00710 PE=3 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 6.1e-178
Identity = 308/330 (93.33%), Postives = 314/330 (95.15%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60
           MELPRF+RPNQ  GSSSS  PILYVANCGPAVGISHP IAAVFAHFGHVKGVH ADDTGA
Sbjct: 1   MELPRFTRPNQHNGSSSS--PILYVANCGPAVGISHPTIAAVFAHFGHVKGVHAADDTGA 60

Query: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120
           RVIVCFSEESSA+AALE LHGRPCPLLGGRTLHIRYSI RPSISQPNDS+SVSLSASELD
Sbjct: 61  RVIVCFSEESSAQAALETLHGRPCPLLGGRTLHIRYSIIRPSISQPNDSVSVSLSASELD 120

Query: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180
           IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP
Sbjct: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180

Query: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240
            FVSHVVDRIS+FPNTED+ADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP
Sbjct: 181 PFVSHVVDRISLFPNTEDVADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240

Query: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300
           CIMEFRRYPEG WH+FP SIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARY WHHYI
Sbjct: 241 CIMEFRRYPEGAWHEFPLSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYAWHHYI 300

Query: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTD 331
           PHHKIDMVKDSSIRR  RRVSFTFRKV  D
Sbjct: 301 PHHKIDMVKDSSIRRAPRRVSFTFRKVGRD 328

BLAST of CSPI04G27590 vs. NCBI nr
Match: XP_004145573.1 (alkylated DNA repair protein ALKBH8 homolog [Cucumis sativus] >KGN55569.1 hypothetical protein Csa_012503 [Cucumis sativus])

HSP 1 Score: 716.8 bits (1849), Expect = 8.7e-203
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60
           MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA
Sbjct: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60

Query: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120
           RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD
Sbjct: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120

Query: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180
           IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP
Sbjct: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180

Query: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240
           SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP
Sbjct: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240

Query: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300
           CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI
Sbjct: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300

Query: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR
Sbjct: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 344

BLAST of CSPI04G27590 vs. NCBI nr
Match: XP_008444260.1 (PREDICTED: alkylated DNA repair protein alkB homolog 8 isoform X1 [Cucumis melo])

HSP 1 Score: 674.1 bits (1738), Expect = 6.5e-190
Identity = 324/344 (94.19%), Postives = 330/344 (95.93%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60
           MELPRF+RPNQ  GSSSS  PILYVANCGPAVGISHP IAAVFAHFGHVKGVH ADDTGA
Sbjct: 1   MELPRFTRPNQHNGSSSS--PILYVANCGPAVGISHPTIAAVFAHFGHVKGVHAADDTGA 60

Query: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120
           RVIVCFSEESSA+AALE LHGRPCPLLGGRTLHIRYSI RPSISQPNDS+SVSLSASELD
Sbjct: 61  RVIVCFSEESSAQAALETLHGRPCPLLGGRTLHIRYSIIRPSISQPNDSVSVSLSASELD 120

Query: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180
           IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP
Sbjct: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180

Query: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240
            FVSHVVDRIS+FPNTED+ADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP
Sbjct: 181 PFVSHVVDRISLFPNTEDVADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240

Query: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300
           CIMEFRRYPEG WH+FP SIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARY WHHYI
Sbjct: 241 CIMEFRRYPEGAWHEFPLSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYAWHHYI 300

Query: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           PHHKIDMVKDSSIRR  RRVSFTFRKVRTDPCQCKFPHYCDSQR
Sbjct: 301 PHHKIDMVKDSSIRRAPRRVSFTFRKVRTDPCQCKFPHYCDSQR 342

BLAST of CSPI04G27590 vs. NCBI nr
Match: XP_038897079.1 (alkylated DNA repair protein ALKBH8 homolog [Benincasa hispida])

HSP 1 Score: 651.0 bits (1678), Expect = 5.8e-183
Identity = 315/344 (91.57%), Postives = 324/344 (94.19%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60
           MELP+F RP +D GSSSSS P LYVANCGPAVGISH  IAAVF  FG VKGVH AD+TGA
Sbjct: 1   MELPKFRRPKEDNGSSSSS-PNLYVANCGPAVGISHRTIAAVFGGFGLVKGVHAADETGA 60

Query: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120
           RVIVCFSEESSARAALEALHGR CPLLGGRTLHIRYSI RPSIS PNDS+SVSLSASELD
Sbjct: 61  RVIVCFSEESSARAALEALHGRLCPLLGGRTLHIRYSIIRPSISHPNDSVSVSLSASELD 120

Query: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180
           IPGLFLLHDFVNAKEEEDLL EVDARPW+NLAKRRVQHYGYEFCYQTRNVNTKHQLGELP
Sbjct: 121 IPGLFLLHDFVNAKEEEDLLMEVDARPWSNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180

Query: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240
           SFVSHVVDRISMFPN ED+ADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP
Sbjct: 181 SFVSHVVDRISMFPNAEDVADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240

Query: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300
           CIMEFRRYPEGTWHK PSS+DLKMENSVNDSNYLR+ IYLPPRSMLLLSGEARY WHHYI
Sbjct: 241 CIMEFRRYPEGTWHKCPSSVDLKMENSVNDSNYLRRVIYLPPRSMLLLSGEARYAWHHYI 300

Query: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           PHHKIDMVKDS+IRRG RRVSFTFRKVRTDPCQCKFPHYCDSQR
Sbjct: 301 PHHKIDMVKDSAIRRGPRRVSFTFRKVRTDPCQCKFPHYCDSQR 343

BLAST of CSPI04G27590 vs. NCBI nr
Match: KAG6591851.1 (Alkylated DNA repair protein ALKBH8-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 641.3 bits (1653), Expect = 4.6e-180
Identity = 311/344 (90.41%), Postives = 321/344 (93.31%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60
           M+LPRF+RP  D GSSSSS P LYVANCGPAVGISH  +AAVF  FG VKGVH AD+TG 
Sbjct: 1   MDLPRFARPRGDNGSSSSS-PNLYVANCGPAVGISHRTVAAVFGDFGDVKGVHAADETGT 60

Query: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120
           RVIVCFSEESSARAALEALHGRPC LLGGRTLHIRYSI RPSISQ NDS+SVSLSASELD
Sbjct: 61  RVIVCFSEESSARAALEALHGRPCSLLGGRTLHIRYSIIRPSISQLNDSVSVSLSASELD 120

Query: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180
           IPGL+LLHDFV AKEEEDLL EVDARPWNNLAKRRVQHYGYEFCYQTRNVNT+HQLGELP
Sbjct: 121 IPGLYLLHDFVTAKEEEDLLLEVDARPWNNLAKRRVQHYGYEFCYQTRNVNTRHQLGELP 180

Query: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240
           SFVSHVVDRISMFPN ED+ADA LDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP
Sbjct: 181 SFVSHVVDRISMFPNAEDVADAILDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240

Query: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300
           CIMEFRRY EGTWHK PSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARY WHHYI
Sbjct: 241 CIMEFRRYTEGTWHKCPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYAWHHYI 300

Query: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           PHHKIDMVKDS+IRRG RRVSFTFRKVRTDPC+CKFPHYCDSQR
Sbjct: 301 PHHKIDMVKDSAIRRGPRRVSFTFRKVRTDPCRCKFPHYCDSQR 343

BLAST of CSPI04G27590 vs. NCBI nr
Match: XP_022936765.1 (alkylated DNA repair protein alkB homolog 8 [Cucurbita moschata])

HSP 1 Score: 641.0 bits (1652), Expect = 6.1e-180
Identity = 310/344 (90.12%), Postives = 322/344 (93.60%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTPILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADDTGA 60
           M+LPRF+RP  D GSSSSS P LYVANCGPAVGISH A+AAVF  FG VKGVH AD+TG 
Sbjct: 1   MDLPRFARPRGDNGSSSSS-PNLYVANCGPAVGISHRAVAAVFGDFGDVKGVHAADETGT 60

Query: 61  RVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSASELD 120
           RVIVCFSEESSARAALEALHGRPC LLGGRTLHIRYSI RPSISQ NDS+SVSLSASELD
Sbjct: 61  RVIVCFSEESSARAALEALHGRPCSLLGGRTLHIRYSIIRPSISQLNDSISVSLSASELD 120

Query: 121 IPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGELP 180
           IPGL+LLHDFV AKEEE+LL EVDARPWNNLAKRRVQHYGYEFCYQTRNVNT+HQLGELP
Sbjct: 121 IPGLYLLHDFVTAKEEEELLSEVDARPWNNLAKRRVQHYGYEFCYQTRNVNTRHQLGELP 180

Query: 181 SFVSHVVDRISMFPNTEDIADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240
           SFVSHVVDRISMFPN ED+ADA LDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP
Sbjct: 181 SFVSHVVDRISMFPNAEDVADAVLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLSLAGP 240

Query: 241 CIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVWHHYI 300
           CIMEFRRY EGTWHK PSSIDLKMENSVNDSNYLR+AIYLPPRSMLLLSGEARY WHHYI
Sbjct: 241 CIMEFRRYTEGTWHKCPSSIDLKMENSVNDSNYLRRAIYLPPRSMLLLSGEARYAWHHYI 300

Query: 301 PHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           PHHKIDMVKDS+IRRG RRVSFTFRKVRTDPC+CKFPHYCDSQR
Sbjct: 301 PHHKIDMVKDSAIRRGPRRVSFTFRKVRTDPCRCKFPHYCDSQR 343

BLAST of CSPI04G27590 vs. TAIR 10
Match: AT1G31600.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 452.2 bits (1162), Expect = 3.7e-127
Identity = 234/349 (67.05%), Postives = 266/349 (76.22%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTP---ILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADD 60
           M  PRF RP Q   SS S  P    LYVANCGPAVG++H AIAAVFA FG V GV+ ADD
Sbjct: 86  MVQPRFVRPTQSSPSSISGEPNSSNLYVANCGPAVGLTHNAIAAVFAEFGEVNGVYAADD 145

Query: 61  TGARVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITR-PSISQPNDSLSVSLSA 120
           +G RVIV F++  SA+AALEAL GRPCP L GR+LHIRYS+ + PS +Q ND + VSL  
Sbjct: 146 SGVRVIVSFADPFSAKAALEALSGRPCPDLKGRSLHIRYSVLQLPSETQVNDCVPVSLID 205

Query: 121 SELDIPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQL 180
           SEL+IPGLFLL DFV   EE+ LL  VDAR W  LAKRRVQHYGYEFCY TRNV+TK +L
Sbjct: 206 SELNIPGLFLLPDFVTVAEEQQLLAAVDARHWIGLAKRRVQHYGYEFCYGTRNVDTKKRL 265

Query: 181 GELPSFVSHVVDRISMFPNTED-IADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSL 240
           GELPSFVS +++RI +FPN ++  A  +LDQLTVNEYP GVGLSPHIDTHSAFE  IFSL
Sbjct: 266 GELPSFVSPILERIYLFPNFDNGSASLNLDQLTVNEYPSGVGLSPHIDTHSAFEDCIFSL 325

Query: 241 SLAGPCIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYV 300
           SLAGPCIMEFRRY   TW    S+ D +      DS+ ++KA+YLPPRSMLLLSGEARY 
Sbjct: 326 SLAGPCIMEFRRYSVSTWK--ASTTDAEKS---GDSSCIKKALYLPPRSMLLLSGEARYA 385

Query: 301 WHHYIPHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           W+HYIPHHKID VKD  IRR  RRVSFT RKVR  PC CK+P YCDSQ+
Sbjct: 386 WNHYIPHHKIDKVKDKVIRRSSRRVSFTLRKVRNHPCSCKYPQYCDSQQ 429

BLAST of CSPI04G27590 vs. TAIR 10
Match: AT1G31600.3 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 452.2 bits (1162), Expect = 3.7e-127
Identity = 234/349 (67.05%), Postives = 266/349 (76.22%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTP---ILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADD 60
           M  PRF RP Q   SS S  P    LYVANCGPAVG++H AIAAVFA FG V GV+ ADD
Sbjct: 86  MVQPRFVRPTQSSPSSISGEPNSSNLYVANCGPAVGLTHNAIAAVFAEFGEVNGVYAADD 145

Query: 61  TGARVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITR-PSISQPNDSLSVSLSA 120
           +G RVIV F++  SA+AALEAL GRPCP L GR+LHIRYS+ + PS +Q ND + VSL  
Sbjct: 146 SGVRVIVSFADPFSAKAALEALSGRPCPDLKGRSLHIRYSVLQLPSETQVNDCVPVSLID 205

Query: 121 SELDIPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQL 180
           SEL+IPGLFLL DFV   EE+ LL  VDAR W  LAKRRVQHYGYEFCY TRNV+TK +L
Sbjct: 206 SELNIPGLFLLPDFVTVAEEQQLLAAVDARHWIGLAKRRVQHYGYEFCYGTRNVDTKKRL 265

Query: 181 GELPSFVSHVVDRISMFPNTED-IADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSL 240
           GELPSFVS +++RI +FPN ++  A  +LDQLTVNEYP GVGLSPHIDTHSAFE  IFSL
Sbjct: 266 GELPSFVSPILERIYLFPNFDNGSASLNLDQLTVNEYPSGVGLSPHIDTHSAFEDCIFSL 325

Query: 241 SLAGPCIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYV 300
           SLAGPCIMEFRRY   TW    S+ D +      DS+ ++KA+YLPPRSMLLLSGEARY 
Sbjct: 326 SLAGPCIMEFRRYSVSTWK--ASTTDAEKS---GDSSCIKKALYLPPRSMLLLSGEARYA 385

Query: 301 WHHYIPHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           W+HYIPHHKID VKD  IRR  RRVSFT RKVR  PC CK+P YCDSQ+
Sbjct: 386 WNHYIPHHKIDKVKDKVIRRSSRRVSFTLRKVRNHPCSCKYPQYCDSQQ 429

BLAST of CSPI04G27590 vs. TAIR 10
Match: AT1G31600.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 447.6 bits (1150), Expect = 9.1e-126
Identity = 232/348 (66.67%), Postives = 264/348 (75.86%), Query Frame = 0

Query: 1   MELPRFSRPNQDYGSSSSSTP---ILYVANCGPAVGISHPAIAAVFAHFGHVKGVHPADD 60
           M  PRF RP Q   SS S  P    LYVANCGPAVG++H AIAAVFA FG V GV+ ADD
Sbjct: 1   MVQPRFVRPTQSSPSSISGEPNSSNLYVANCGPAVGLTHNAIAAVFAEFGEVNGVYAADD 60

Query: 61  TGARVIVCFSEESSARAALEALHGRPCPLLGGRTLHIRYSITRPSISQPNDSLSVSLSAS 120
           +G RVIV F++  SA+AALEAL GRPCP L GR+LHIRYS+ +   S+ ND + VSL  S
Sbjct: 61  SGVRVIVSFADPFSAKAALEALSGRPCPDLKGRSLHIRYSVLQLP-SEVNDCVPVSLIDS 120

Query: 121 ELDIPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLG 180
           EL+IPGLFLL DFV   EE+ LL  VDAR W  LAKRRVQHYGYEFCY TRNV+TK +LG
Sbjct: 121 ELNIPGLFLLPDFVTVAEEQQLLAAVDARHWIGLAKRRVQHYGYEFCYGTRNVDTKKRLG 180

Query: 181 ELPSFVSHVVDRISMFPNTED-IADASLDQLTVNEYPPGVGLSPHIDTHSAFEGLIFSLS 240
           ELPSFVS +++RI +FPN ++  A  +LDQLTVNEYP GVGLSPHIDTHSAFE  IFSLS
Sbjct: 181 ELPSFVSPILERIYLFPNFDNGSASLNLDQLTVNEYPSGVGLSPHIDTHSAFEDCIFSLS 240

Query: 241 LAGPCIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSGEARYVW 300
           LAGPCIMEFRRY   TW    S+ D +      DS+ ++KA+YLPPRSMLLLSGEARY W
Sbjct: 241 LAGPCIMEFRRYSVSTWK--ASTTDAEKS---GDSSCIKKALYLPPRSMLLLSGEARYAW 300

Query: 301 HHYIPHHKIDMVKDSSIRRGRRRVSFTFRKVRTDPCQCKFPHYCDSQR 345
           +HYIPHHKID VKD  IRR  RRVSFT RKVR  PC CK+P YCDSQ+
Sbjct: 301 NHYIPHHKIDKVKDKVIRRSSRRVSFTLRKVRNHPCSCKYPQYCDSQQ 342

BLAST of CSPI04G27590 vs. TAIR 10
Match: AT4G02485.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.0e-12
Identity = 58/219 (26.48%), Postives = 92/219 (42.01%), Query Frame = 0

Query: 120 DIPGLFLLHDFVNAKEEEDLLREVDARPWNNLAKRRVQHYGYEFCYQTRNVNTKHQLGEL 179
           +I GL+L  +F++   +  LL  +    W                +   ++N   + G+L
Sbjct: 43  EINGLWLYRNFLSIAHQSHLLSAILNEGW----------------FVEESINQAMRFGDL 102

Query: 180 PSFVSHVVDRISMFPNTEDIADAS---------LDQLTVNEYPPGVGLSPHIDTHSAFEG 239
           PS+ + + D I     + D+   S          DQL VN Y PG G+  H+D    FE 
Sbjct: 103 PSWATELSDLIRETLESVDLPVLSADLLWREPLFDQLIVNLYQPGEGICAHVDL-LRFED 162

Query: 240 LIFSLSLAGPCIMEFRRYPEGTWHKFPSSIDLKMENSVNDSNYLRKAIYLPPRSMLLLSG 299
            I  +SL  PC+M F                    +    + Y    + L P S++L+SG
Sbjct: 163 GIAIVSLESPCVMRF--------------------SPAEKNEYEAVDVLLNPGSLILMSG 222

Query: 300 EARYVWHHYI--PHHKIDMVKDSSIRRGRRRVSFTFRKV 328
           EARY W H I    +   + +   I + +RR+S T RK+
Sbjct: 223 EARYRWKHEINRKQNGFQLWEGEEIDQ-KRRISITLRKL 223

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RWY15.2e-12667.05Alkylated DNA repair protein ALKBH8 homolog OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q07G102.3e-3632.56Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alk... [more]
Q95K794.0e-3335.37Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=AL... [more]
Q96BT72.6e-3234.55Alkylated DNA repair protein alkB homolog 8 OS=Homo sapiens OX=9606 GN=ALKBH8 PE... [more]
Q80Y204.4e-3234.54Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 P... [more]
Match NameE-valueIdentityDescription
A0A0A0L3H64.2e-203100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G669210 PE=3 SV=1[more]
A0A1S3B9H03.1e-19094.19alkylated DNA repair protein alkB homolog 8 isoform X1 OS=Cucumis melo OX=3656 G... [more]
A0A6J1FEN42.9e-18090.12alkylated DNA repair protein alkB homolog 8 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1IPP92.1e-17889.24alkylated DNA repair protein alkB homolog 8 isoform X1 OS=Cucurbita maxima OX=36... [more]
A0A5D3BS486.1e-17893.33Alkylated DNA repair protein alkB-like protein 8 isoform X1 OS=Cucumis melo var.... [more]
Match NameE-valueIdentityDescription
XP_004145573.18.7e-203100.00alkylated DNA repair protein ALKBH8 homolog [Cucumis sativus] >KGN55569.1 hypoth... [more]
XP_008444260.16.5e-19094.19PREDICTED: alkylated DNA repair protein alkB homolog 8 isoform X1 [Cucumis melo][more]
XP_038897079.15.8e-18391.57alkylated DNA repair protein ALKBH8 homolog [Benincasa hispida][more]
KAG6591851.14.6e-18090.41Alkylated DNA repair protein ALKBH8-like protein, partial [Cucurbita argyrosperm... [more]
XP_022936765.16.1e-18090.12alkylated DNA repair protein alkB homolog 8 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G31600.13.7e-12767.05RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT1G31600.33.7e-12767.05RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT1G31600.29.1e-12666.67RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT4G02485.11.0e-1226.482-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 7..114
e-value: 3.0E-8
score: 35.6
IPR027450Alpha-ketoglutarate-dependent dioxygenase AlkB-likePFAMPF135322OG-FeII_Oxy_2coord: 159..325
e-value: 1.0E-16
score: 61.7
IPR037151Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyGENE3D2.60.120.590coord: 116..333
e-value: 8.6E-48
score: 164.4
NoneNo IPR availablePANTHERPTHR13069:SF8ALKYLATED DNA REPAIR PROTEIN ALKBH8 HOMOLOGcoord: 18..341
NoneNo IPR availablePANTHERPTHR13069UNCHARACTERIZEDcoord: 18..341
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 121..328
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 203..328
score: 9.205117
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 21..99
score: 10.036136
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 7..107

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G27590.1CSPI04G27590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding