CSPI04G25670 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G25670
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMechanosensitive ion channel protein
LocationChr4: 22970605 .. 22973876 (+)
RNA-Seq ExpressionCSPI04G25670
SyntenyCSPI04G25670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTATCTTTTTGTTTTAGTTGAAGCGAAGTGGGTTTCTGTTCTTAGACATTGCTTCTGCTTGTGGGTGTGATTTTGTATTCAATCCACTCTCTCTGCCTCTGTTTTCTGGTAAGTTTACCTTTAACAATGTGGGTTTTGCTGGGTCTTTTTGTGATGCAAGTTTTCTTTATTAAGCTTGATGATTTTGATTGTTGCGTCTTGGTTTGCTCTTAATGACCCTGTTCTTCGATTTCGTTTGTTTAGAGCTAGGTTTTGATTCTGATGGATGTGAACGGCAATAAACCAAAAGTCGTTCGAAGAAGTTTGTCTCAGAAGGAAGGTGAAAATGCTGGCCAAGTTGTGGTTGAAATTAGCAATAGCAGCGTTGTGTTTTCGAAAGAAACCAGAGATGGAAATAACTACTCTGTACTGAAACAAGACAGAGTCGATTCACAGAACAAAGAGTCGACTGGTTCGAGCATTGACCATGGCTTCGATTCACATATTCCTCCCACTGCCAATGAGCCCTTGAAAATTCCCAGTTCAAATAGAACCCTCACGCCTAGAAGATCTCTCAAAAGATCAATTCTATCGAGACCCAAATCCAGATTTGGGGAACAGTCACGTTACACCGATTCAGATGATAAGTTTGAGGAGAAACATGAGTCGTTGAGAGAACAAACCGGTGCAACTTCATCCAGAAGCTCTTCTCTTAACACGCCGAAGGCCCAACCTGAGGAAGAAGATGAAGAGGATATAGTTAAGACAGAGCAGTTGAACAAAAAGCACAAGAAATGTAAGGTGAAGACAGTGATTAAGTGGATTGTAGTTTTTTGCCTAATTGGGTGCTTGGTGGCTAGTTTGACCGTTAACCGTTTGAAGAATTGCTTCTTTTTGGGTTTGGAGATTTGGAAATGGTGTTTACTTGCTACTGTGATTTTTTGTGGATTGATACTTACTCATTGGGCTATGAATGTGGTTGTCAGTTTGATTGAGGGAAACTTTTTGCTAAAGAAAAAAGTGCTCTATTTTGTTCATGGGTTAAAGAAGAGTGTCCAAGTGACCCTTTGGTTGGCATCGGTTCTTAGCACATGGGAGCCGTTGTTCAATCAGCGTAACCATAGGAGTTCAAGAACCACTGGGAAGATTCTGGATGCTATTACGTGGACTCTTGTTGCTCTTCTTATAGGGTCATTCCTATGGTTGGTAAAAACATTGTTGTTGAAAATACTGGCATCCAAGTTCCATAAGGATCGATTTTTCGACAGAATTCAGGAATCCATTTTCCATCATCATGTTCTACAAGCCCTCTTGGGGCCTCCATTAATGCAGGAAGTTGAAAGTGCTGCCAAGTTCAGCCGTTGTCTATTTAGTTGGGAGAATAAAAAATCAGACCTTAAGAAGATAATTGACACGGGAAAGATTCACCACCTGCAGCGAGAAAAAGTTTCATCTTGGACAATGAAGGTGTTGGTTGAGGCAGTTACAAGTTCGGCGATGTCAATCTCACAAATACTTGATGAAAGCTACTATAATGTTGACGATGGCGAGATTGACCATGAAATGGAAATTGCCAGTGTCGTTGCCTCCAAGATCCTTAGAAATGTTGCTCTTCCTGGGAAAAAGTGAGACACTTTGGTGTTTGCTTGTGATCCTTTTGTTCAAACTCATGGTCATGTTAATGTTTTAACCTTTCCCTTTTGTTAGGTTCATACAGGAAGAGGATCTTCTGCAATTCGTGGTCAAAGAAGAAATCGATCTTGTGTTACCACACTTCGAGGTAGATGAGACAAAGAGGATTGGCAAGAAAGCTCTCAAAAAATGGGTGGTGAGAACTTGTATCTATTATTTAACTTTCTTCACATCGAGCTATCTAGCCACTCCAACGCATTAGAAGCAGAATTCACTCAGGCCCCATTTAGTCCAAATGAAATGAGCAATCAAAAGAGAGATTTTCTTAAAATTAAAAATCATTTCTGATTTTGTTGGGGGATACGCAAATCTTGATTCATTCGTGATTTATGTTAAACAACATTGAGAATCGTTCTCAATTCTCATTTGATCATTCCCAATAAGCAAACAAAGTGTTAGAAGTTAGAACCTAATAAACGCGTAGAGTTCTTGTGTTTCTGTGAACTCCAAAGCCCTCATGGTTTTGGCGAAAAAATTATTTCCCCTTTCTTCTTGTCTTCAACAATTTCCTCAATTGTCATCGATAGCCGAAATGTCTCGACAGTGTTCTATGTTCTGTAGTAACTTCTTTTCGTGCAGGTGAAGGTTTTTCAAGAGAGAAAAACTCTAGCACATGCCTTGAAAGACACTAAAACTGCTGTGAAGCAATTGAATAATTTGGTAACAGCGGTTGTTATAATAGTAATGGCTGTTATTTGGCTTCTGTTGATGGAAATTGCTACATCCAAAGTACTCGTTTTCCTTCTATCTCAACTTGCAGTGGCAGCTTTCATGTTCGGAAACGCTTGCAAGACTACATTTGAAGCTCTAATCTTTGTGTTTGTTATGCATCCATTTGATGTCGGGGACCGTTGTGTTGTCGATGGCATCCCGGTAAGCTTTTTAACTTGCTCTCATGAAAGTCTCATGTTTCTTTTCTGTTTTTAACTTATGGCAACAACCTGCAGCTGTTGGTTGAAGAAATGAACATCTTGACGACAGTCTTCTTGAAACTAAACAATGAGAAGGTGTATTATCCCAACTCAGTCTTGGCAACAAAGCCCATCAGTAACTACTACAGAAGTCCAGACATGAGCGAGACCACCGAATTCTCGATCAACTTCGCGACGCCACTAGAGAGGATTGGAGCCATGAAAGAAAAAATAAAGAGGTGGGTGGAAGTGAAACAAACAATGAAGATTATTTCAAATACACTAATATATCGATCATTGTGCAACGAACGAAAAACCATTGTCTTCTAATAAATCCTTATGAATGGCTTCAGGTATTTGGAGAAAAATCCACAACACTGGCGTCCAAGTCATCTTATGGTGGTGAAAGAGATCGAAAACGTGAATGAGATCAAGATTGCTCTTTATTCTACCCATACCATGAGTTATCAAGACTATGGTGAGAAGATGAAACGAAGATCCGAGCTGGTGATGGAGTTGAAGAGAATATTTGAAGAACTGAAGATCAACTACACTCTTCTGCCTCAAACAATTCATCTCTTTCCAGTTGAATCACACTAAAGCTTCTGATACTTTTGACAAAGGAAATGGTTAGCTTCTTTTAGCTGCTTTGTTAACTTAGGCAAAGCTACTAATTCGAAAATATAAC

mRNA sequence

TTCTATCTTTTTGTTTTAGTTGAAGCGAAGTGGGTTTCTGTTCTTAGACATTGCTTCTGCTTGTGGGTGTGATTTTGTATTCAATCCACTCTCTCTGCCTCTGTTTTCTGAGCTAGGTTTTGATTCTGATGGATGTGAACGGCAATAAACCAAAAGTCGTTCGAAGAAGTTTGTCTCAGAAGGAAGGTGAAAATGCTGGCCAAGTTGTGGTTGAAATTAGCAATAGCAGCGTTGTGTTTTCGAAAGAAACCAGAGATGGAAATAACTACTCTGTACTGAAACAAGACAGAGTCGATTCACAGAACAAAGAGTCGACTGGTTCGAGCATTGACCATGGCTTCGATTCACATATTCCTCCCACTGCCAATGAGCCCTTGAAAATTCCCAGTTCAAATAGAACCCTCACGCCTAGAAGATCTCTCAAAAGATCAATTCTATCGAGACCCAAATCCAGATTTGGGGAACAGTCACGTTACACCGATTCAGATGATAAGTTTGAGGAGAAACATGAGTCGTTGAGAGAACAAACCGGTGCAACTTCATCCAGAAGCTCTTCTCTTAACACGCCGAAGGCCCAACCTGAGGAAGAAGATGAAGAGGATATAGTTAAGACAGAGCAGTTGAACAAAAAGCACAAGAAATGTAAGGTGAAGACAGTGATTAAGTGGATTGTAGTTTTTTGCCTAATTGGGTGCTTGGTGGCTAGTTTGACCGTTAACCGTTTGAAGAATTGCTTCTTTTTGGGTTTGGAGATTTGGAAATGGTGTTTACTTGCTACTGTGATTTTTTGTGGATTGATACTTACTCATTGGGCTATGAATGTGGTTGTCAGTTTGATTGAGGGAAACTTTTTGCTAAAGAAAAAAGTGCTCTATTTTGTTCATGGGTTAAAGAAGAGTGTCCAAGTGACCCTTTGGTTGGCATCGGTTCTTAGCACATGGGAGCCGTTGTTCAATCAGCGTAACCATAGGAGTTCAAGAACCACTGGGAAGATTCTGGATGCTATTACGTGGACTCTTGTTGCTCTTCTTATAGGGTCATTCCTATGGTTGGTAAAAACATTGTTGTTGAAAATACTGGCATCCAAGTTCCATAAGGATCGATTTTTCGACAGAATTCAGGAATCCATTTTCCATCATCATGTTCTACAAGCCCTCTTGGGGCCTCCATTAATGCAGGAAGTTGAAAGTGCTGCCAAGTTCAGCCGTTGTCTATTTAGTTGGGAGAATAAAAAATCAGACCTTAAGAAGATAATTGACACGGGAAAGATTCACCACCTGCAGCGAGAAAAAGTTTCATCTTGGACAATGAAGGTGTTGGTTGAGGCAGTTACAAGTTCGGCGATGTCAATCTCACAAATACTTGATGAAAGCTACTATAATGTTGACGATGGCGAGATTGACCATGAAATGGAAATTGCCAGTGTCGTTGCCTCCAAGATCCTTAGAAATGTTGCTCTTCCTGGGAAAAAGTTCATACAGGAAGAGGATCTTCTGCAATTCGTGGTCAAAGAAGAAATCGATCTTGTGTTACCACACTTCGAGGTAGATGAGACAAAGAGGATTGGCAAGAAAGCTCTCAAAAAATGGGTGGTGAAGGTTTTTCAAGAGAGAAAAACTCTAGCACATGCCTTGAAAGACACTAAAACTGCTGTGAAGCAATTGAATAATTTGGTAACAGCGGTTGTTATAATAGTAATGGCTGTTATTTGGCTTCTGTTGATGGAAATTGCTACATCCAAAGTACTCGTTTTCCTTCTATCTCAACTTGCAGTGGCAGCTTTCATGTTCGGAAACGCTTGCAAGACTACATTTGAAGCTCTAATCTTTGTGTTTGTTATGCATCCATTTGATGTCGGGGACCGTTGTGTTGTCGATGGCATCCCGCTGTTGGTTGAAGAAATGAACATCTTGACGACAGTCTTCTTGAAACTAAACAATGAGAAGGTGTATTATCCCAACTCAGTCTTGGCAACAAAGCCCATCAGTAACTACTACAGAAGTCCAGACATGAGCGAGACCACCGAATTCTCGATCAACTTCGCGACGCCACTAGAGAGGATTGGAGCCATGAAAGAAAAAATAAAGAGGTATTTGGAGAAAAATCCACAACACTGGCGTCCAAGTCATCTTATGGTGGTGAAAGAGATCGAAAACGTGAATGAGATCAAGATTGCTCTTTATTCTACCCATACCATGAGTTATCAAGACTATGGTGAGAAGATGAAACGAAGATCCGAGCTGGTGATGGAGTTGAAGAGAATATTTGAAGAACTGAAGATCAACTACACTCTTCTGCCTCAAACAATTCATCTCTTTCCAGTTGAATCACACTAAAGCTTCTGATACTTTTGACAAAGGAAATGGTTAGCTTCTTTTAGCTGCTTTGTTAACTTAGGCAAAGCTACTAATTCGAAAATATAAC

Coding sequence (CDS)

ATGGATGTGAACGGCAATAAACCAAAAGTCGTTCGAAGAAGTTTGTCTCAGAAGGAAGGTGAAAATGCTGGCCAAGTTGTGGTTGAAATTAGCAATAGCAGCGTTGTGTTTTCGAAAGAAACCAGAGATGGAAATAACTACTCTGTACTGAAACAAGACAGAGTCGATTCACAGAACAAAGAGTCGACTGGTTCGAGCATTGACCATGGCTTCGATTCACATATTCCTCCCACTGCCAATGAGCCCTTGAAAATTCCCAGTTCAAATAGAACCCTCACGCCTAGAAGATCTCTCAAAAGATCAATTCTATCGAGACCCAAATCCAGATTTGGGGAACAGTCACGTTACACCGATTCAGATGATAAGTTTGAGGAGAAACATGAGTCGTTGAGAGAACAAACCGGTGCAACTTCATCCAGAAGCTCTTCTCTTAACACGCCGAAGGCCCAACCTGAGGAAGAAGATGAAGAGGATATAGTTAAGACAGAGCAGTTGAACAAAAAGCACAAGAAATGTAAGGTGAAGACAGTGATTAAGTGGATTGTAGTTTTTTGCCTAATTGGGTGCTTGGTGGCTAGTTTGACCGTTAACCGTTTGAAGAATTGCTTCTTTTTGGGTTTGGAGATTTGGAAATGGTGTTTACTTGCTACTGTGATTTTTTGTGGATTGATACTTACTCATTGGGCTATGAATGTGGTTGTCAGTTTGATTGAGGGAAACTTTTTGCTAAAGAAAAAAGTGCTCTATTTTGTTCATGGGTTAAAGAAGAGTGTCCAAGTGACCCTTTGGTTGGCATCGGTTCTTAGCACATGGGAGCCGTTGTTCAATCAGCGTAACCATAGGAGTTCAAGAACCACTGGGAAGATTCTGGATGCTATTACGTGGACTCTTGTTGCTCTTCTTATAGGGTCATTCCTATGGTTGGTAAAAACATTGTTGTTGAAAATACTGGCATCCAAGTTCCATAAGGATCGATTTTTCGACAGAATTCAGGAATCCATTTTCCATCATCATGTTCTACAAGCCCTCTTGGGGCCTCCATTAATGCAGGAAGTTGAAAGTGCTGCCAAGTTCAGCCGTTGTCTATTTAGTTGGGAGAATAAAAAATCAGACCTTAAGAAGATAATTGACACGGGAAAGATTCACCACCTGCAGCGAGAAAAAGTTTCATCTTGGACAATGAAGGTGTTGGTTGAGGCAGTTACAAGTTCGGCGATGTCAATCTCACAAATACTTGATGAAAGCTACTATAATGTTGACGATGGCGAGATTGACCATGAAATGGAAATTGCCAGTGTCGTTGCCTCCAAGATCCTTAGAAATGTTGCTCTTCCTGGGAAAAAGTTCATACAGGAAGAGGATCTTCTGCAATTCGTGGTCAAAGAAGAAATCGATCTTGTGTTACCACACTTCGAGGTAGATGAGACAAAGAGGATTGGCAAGAAAGCTCTCAAAAAATGGGTGGTGAAGGTTTTTCAAGAGAGAAAAACTCTAGCACATGCCTTGAAAGACACTAAAACTGCTGTGAAGCAATTGAATAATTTGGTAACAGCGGTTGTTATAATAGTAATGGCTGTTATTTGGCTTCTGTTGATGGAAATTGCTACATCCAAAGTACTCGTTTTCCTTCTATCTCAACTTGCAGTGGCAGCTTTCATGTTCGGAAACGCTTGCAAGACTACATTTGAAGCTCTAATCTTTGTGTTTGTTATGCATCCATTTGATGTCGGGGACCGTTGTGTTGTCGATGGCATCCCGCTGTTGGTTGAAGAAATGAACATCTTGACGACAGTCTTCTTGAAACTAAACAATGAGAAGGTGTATTATCCCAACTCAGTCTTGGCAACAAAGCCCATCAGTAACTACTACAGAAGTCCAGACATGAGCGAGACCACCGAATTCTCGATCAACTTCGCGACGCCACTAGAGAGGATTGGAGCCATGAAAGAAAAAATAAAGAGGTATTTGGAGAAAAATCCACAACACTGGCGTCCAAGTCATCTTATGGTGGTGAAAGAGATCGAAAACGTGAATGAGATCAAGATTGCTCTTTATTCTACCCATACCATGAGTTATCAAGACTATGGTGAGAAGATGAAACGAAGATCCGAGCTGGTGATGGAGTTGAAGAGAATATTTGAAGAACTGAAGATCAACTACACTCTTCTGCCTCAAACAATTCATCTCTTTCCAGTTGAATCACACTAA

Protein sequence

MDVNGNKPKVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQNKESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSDDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVKTVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFSRCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNVDDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKINYTLLPQTIHLFPVESH*
Homology
BLAST of CSPI04G25670 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 1.9e-178
Identity = 363/685 (52.99%), Postives = 485/685 (70.80%), Query Frame = 0

Query: 73  SHIPPTANEPLKIPSSNRT-LTPRRSLKRSILSRPKSRFGEQSRYTDSDDKFEEKHESLR 132
           S +  + N+P + P+ N   LT R+S  RS+ S+PKSRF + S   D+    EE    +R
Sbjct: 52  SKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VR 111

Query: 133 EQTGA--TSSRSSSLN------------TP-KAQPEEEDEEDIVKTEQLNKK-HKKCKVK 192
           EQ GA  + SR+S  N            TP K   E++++E+I K  +LN++   K    
Sbjct: 112 EQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTL 171

Query: 193 TVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVS 252
            +I+      ++  LVASLT+N LK+  F GLE+WKWC+L  VIF G+++T+W M ++V 
Sbjct: 172 ALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVF 231

Query: 253 LIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITW 312
           LIE NFLL++KVLYFVHGLKKSVQV +WL  +L  W  LFN    RS   T K+L  IT 
Sbjct: 232 LIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAAT-KVLKCITR 291

Query: 313 TLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESA 372
           TL+++L G+F WLVKTLLLKILA+ F+ + FFDRIQ+S+FH +VLQ L G PLM+E E  
Sbjct: 292 TLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERV 351

Query: 373 AK---FSRCLFSWENKKSDL--KKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMS-IS 432
            +        F+   KK  +  KK+ID GK+H ++REKVS+WTM+VL+EAV +S +S IS
Sbjct: 352 GREPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTIS 411

Query: 433 QILDESYYNVD----DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEID 492
             LDE+ Y       D EI  EME A   A  + RNVA P   +I+EEDLL+F++KEE+D
Sbjct: 412 DTLDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVD 471

Query: 493 LVLPHFE-VDETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVM 552
           LV P F+   ET RI +KA  +WVVKV+  R+ LAH+L DTKTAVKQLN LVTA++++V 
Sbjct: 472 LVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVT 531

Query: 553 AVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG 612
            VIWLLL+E+AT+KVL+F  +QL   AF+ G+ CK  FE+++FVFVMHP+DVGDRCVVDG
Sbjct: 532 VVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDG 591

Query: 613 IPLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLER 672
           + +LVEEMN+LTTVFLKLNNEKVYYPN+VLATKPISNY+RSP+M ET EFSI+F+TP+ +
Sbjct: 592 VAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSK 651

Query: 673 IGAMKEKIKRYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSE 730
           I  +KE+I  YLE+NPQHW P H +VVKEIEN+N++K+ALYS HT+++Q+  E+  RR+E
Sbjct: 652 IAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTE 711

BLAST of CSPI04G25670 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 3.4e-159
Identity = 334/747 (44.71%), Postives = 488/747 (65.33%), Query Frame = 0

Query: 15  LSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDS---QNKESTGSSIDHGF 74
           ++++   N  +VV+ +S+     SK+ R   +++ L     D+   ++K     SI    
Sbjct: 1   MAERRVSNGEEVVINVSDKE--DSKDPRASPSFNPLASPDSDAGIEKSKPVPPISIPTPE 60

Query: 75  DSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSDDKFEEKHESLR 134
                 + ++P KIPS    L  R+SL RSI S+PKSRFGEQ  +     + E    SLR
Sbjct: 61  IYKFSGSVHKPPKIPSP-EGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLR 120

Query: 135 EQTGATS-----------------SRSSSLNTPKAQPEEEDEEDIVKTEQLNK-KHKKCK 194
           EQ GA S                 S +S+  +  A+ E ++ E+I K  +L++ K    K
Sbjct: 121 EQFGAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMK 180

Query: 195 VKTVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVV 254
               ++ +V   ++G L+ SLT++ +      GLE WKWC+L  V   G+++T+W M+ V
Sbjct: 181 PLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFV 240

Query: 255 VSLIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAI 314
           V +IE N+LL+KKVLYFVHGLKK+VQV +W + VL  W  LF+  + + +R T + LD I
Sbjct: 241 VFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFD-GDVKRTRKTKRFLDFI 300

Query: 315 TWTLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVE 374
           TWT+V+LL+GS L+LVKT  LK+LASKF+   FF+RIQES+FH +VLQ L GPPL++E E
Sbjct: 301 TWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEAE 360

Query: 375 SAAK--------FSRCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAV-TSS 434
           +  +        F+R   + + K  D KK+ID GK+H +++EKVS+WTM+VL+EAV TS 
Sbjct: 361 NVGRVPSTGHLSFTR---TKDGKVKD-KKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSG 420

Query: 435 AMSISQILDESYYNVD--DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKE 494
             +IS  LDE     +  D EI +EME A   A  +  NVA P   +I+E+DLL+F++KE
Sbjct: 421 ISTISSTLDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKE 480

Query: 495 EIDLVLPHFEVDETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVII 554
           E+DLVLP  E  +T +I +K   +WVV V+  RKT+ H+L DTKTAVKQL+ L+T ++ +
Sbjct: 481 EVDLVLPLIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILTV 540

Query: 555 VMAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVV 614
           +  ++W++L++IA++K+L+   SQ    AFM G+ CK  FE+ +FVFVMHP+DVGDRCVV
Sbjct: 541 ITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCVV 600

Query: 615 DGIPLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPL 674
           DG+ LLVEE+++LTTVFLK++NEKV+YPNSVL +KPISN+YRSPDM +  +F I F+TP 
Sbjct: 601 DGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTPA 660

Query: 675 ERIGAMKEKIKRYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRR 730
           E+IG +K KI  YL  N QHW P   ++V+ IEN+N++ + +   HT+++Q Y EK  RR
Sbjct: 661 EKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLRR 720

BLAST of CSPI04G25670 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.0e-122
Identity = 269/676 (39.79%), Postives = 405/676 (59.91%), Query Frame = 0

Query: 99  KRSILSRPKSRFGEQSRYTDSDDKFEEKHESLREQTGATSS----RSSSLNTPKAQP-EE 158
           K+ +LSR K++   Q   T +    ++       ++G   S    +S    TP     EE
Sbjct: 185 KKPMLSRNKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEE 244

Query: 159 EDEEDIVKTEQLNKKHKKCKVK--TVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWK 218
           E+EED    E L ++ K+ K+     ++WI +  ++  LV SLT++ L+   +  L++WK
Sbjct: 245 EEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWK 304

Query: 219 WCLLATVIFCGLILTHWAMNVVVSLIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTW 278
           W +   V+ CG +++ W + ++V L+E NF  +K+VLYFV+G++KSVQ  LWL  VL  W
Sbjct: 305 WEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAW 364

Query: 279 EPLFNQRNHRSSRTTGKILDAITWTLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQ 338
             LF+++  R +R+T   L  +T  LV LL+   +WLVKT+L+K+LAS FH   +FDRIQ
Sbjct: 365 HFLFDKKVERETRSTA--LRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQ 424

Query: 339 ESIFHHHVLQALLGPPLM-------------QEVESAAKFSRCLFSWENKKSDLKKIIDT 398
           ES+F  +V++ L GPPLM             ++V+S  K +         K+ +K  +  
Sbjct: 425 ESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLP-PALKATVKSFMKV 484

Query: 399 GKIHHLQR----------------------EKVSSWTMKVLVEAVTSSAMSI--SQILDE 458
           GK   L R                      + VS+W MK L+  +   A+S     + D 
Sbjct: 485 GKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDT 544

Query: 459 SYYNVDDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFE-V 518
           +  + D   I  E E A   A KI  NV  PG ++I  ED L+F+ +EE +  +  FE  
Sbjct: 545 TQEDEDATHIRSEYE-AKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGA 604

Query: 519 DETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLME 578
            E+ +I K  LK WVVK F+ER+ LA  L DTKTAV +L+ ++  V+ I++ +IWLL++ 
Sbjct: 605 SESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILG 664

Query: 579 IATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMN 638
           IAT++ L+ L SQL + AF+FGN+CKT FEA+IF+FVMHPFDVGDRC +DG+ L+VEEMN
Sbjct: 665 IATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMN 724

Query: 639 ILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIK 698
           ILTTVFL+ +N+K+ YPNSVL TKPI+NYYRSPDM +  EF ++ ATP E+I A+K++I 
Sbjct: 725 ILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRIL 784

Query: 699 RYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIF 730
            Y++    +W P+ ++V   ++++N +KIA++ TH M++QD GE+  RR  L+ E+ +  
Sbjct: 785 SYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTC 844

BLAST of CSPI04G25670 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 9.8e-122
Identity = 281/713 (39.41%), Postives = 421/713 (59.05%), Query Frame = 0

Query: 56  DSQNKESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSR 115
           D   +  + S+ ++  D  +   +     I  S+ TL   R+  RS LS P +    Q  
Sbjct: 132 DPVGRRDSRSNTNNNDDGEVVKCSGNNAPIQRSSSTLLKMRT--RSRLSDPPT---PQLP 191

Query: 116 YTDSDDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVK 175
              +D K     +S + ++G          +PK Q EEE E+D    E L ++++K K+ 
Sbjct: 192 PQTADMKSGRIPKSGQMKSGFFG------KSPKTQGEEE-EDDPFAAEDLPEEYRKDKLS 251

Query: 176 --TVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVV 235
              V++W+ +  +I   V +L +  L+      L++WKW  +  V+ CG +++ W + +V
Sbjct: 252 LWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIV 311

Query: 236 VSLIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAI 295
           V  IE NFLL+K+VLYFV+G++K+VQ  LWL  VL  W  LF+++  +++ T  K L  +
Sbjct: 312 VFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANT--KALRVV 371

Query: 296 TWTLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQ--- 355
           T   V LL+G  LWLVKTLL+K+LAS FH   +FDRIQES+F  +V++ L GPPL++   
Sbjct: 372 TKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQK 431

Query: 356 EVESAAKFSRCLFSWEN----------KKSDLK---------------------KIIDTG 415
             E   + S  +  ++N          +KS +K                     K I   
Sbjct: 432 NEEEEERISVEVKKFQNPGGVEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITID 491

Query: 416 KIHHLQREKVSSWTMKVLVEAVTSSAMSI--SQILDESYYNVDDGEIDHEMEIASVVASK 475
            +H L  + VS+W MK L+  + + +++    Q+ D S  +    +I  E E A + A K
Sbjct: 492 SLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFE-AKLAARK 551

Query: 476 ILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFE-VDETKRIGKKALKKWVVKVFQERK 535
           I  NVA PG KFI   D+++F+  +E    L  FE   ET RI K +LK WVV  F+ER+
Sbjct: 552 IFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERR 611

Query: 536 TLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGN 595
            LA  L DTKTAV +L+ +V  VV I++ VIWL+++ I ++K LV + SQ+ V AF+FGN
Sbjct: 612 ALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGN 671

Query: 596 ACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTTVFLKLNNEKVYYPNSVLAT 655
            CK  FE++I++FV+HPFDVGDRC +DG+ ++VEEMNILTTVFL+ +N+KV YPNS+L T
Sbjct: 672 MCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWT 731

Query: 656 KPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKNPQHWRPSHLMVVKEIEN 715
           K I NYYRSPDM +  EFSI+  TP E+I  +K++I  Y+E    HW P+ ++V K++E+
Sbjct: 732 KSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMES 791

Query: 716 VNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKINYTLLPQTIHL 730
           +N ++IA++ TH M++QD GEK  RRS+LV E+ +I  EL I Y L P  I++
Sbjct: 792 LNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINV 829

BLAST of CSPI04G25670 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.8e-120
Identity = 259/647 (40.03%), Postives = 384/647 (59.35%), Query Frame = 0

Query: 138 SSRSSSLNTPK--AQPEEEDEEDIVKTEQLN---KKHKKCKVKTVIKWIVVFCLIGCLVA 197
           S R++   TP      + E+EED    E L    +K K C V  +I+WI +  +I  L+ 
Sbjct: 217 SGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKIC-VWVIIEWIFLILIIASLIC 276

Query: 198 SLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGNFLLKKKVLYFVH 257
           SL +  L+      L +WKW ++  V+ CG +++ W + + V  +E NFL +KKVLYFV+
Sbjct: 277 SLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVY 336

Query: 258 GLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVALLIGSFLWLVKTL 317
           G++K VQ  LWL  VL  W  LF+++  R  R+T  +L  +T  L+ LL+   +WL+KTL
Sbjct: 337 GIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRST--VLKYVTKVLICLLVAVIIWLIKTL 396

Query: 318 LLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQ---EVESAAKFSRCLFSWENKK 377
           L+K+LAS FH   +FDRIQES+F  +V++ L GPP ++   E E  A   +       K 
Sbjct: 397 LVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKL 456

Query: 378 SDL---------KKIIDTG-----------------------------KIHHLQR---EK 437
           S L         +  + +G                             +I HLQR   + 
Sbjct: 457 SPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKN 516

Query: 438 VSSWTMKVLVEAVTSSAMSISQILDESYYNVDDGEIDHEMEI-----ASVVASKILRNVA 497
           VS+W MK L+  +    +S    LDE   +    E D   +I     A + A KI +NVA
Sbjct: 517 VSAWKMKKLMNVIKKGTLS---TLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVA 576

Query: 498 LPGKKFIQEEDLLQFVVKEEIDLVLPHFE-VDETKRIGKKALKKWVVKVFQERKTLAHAL 557
            PG ++I  ED ++F+ ++E +  +  FE   E  +I K  LK WVV  F+ER+ LA  L
Sbjct: 577 EPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTL 636

Query: 558 KDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTF 617
            DTKTAV +L+ +V  +V IV+ +IWLL++ IAT+K L+ + SQL +  F+FGN+CKT F
Sbjct: 637 NDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIF 696

Query: 618 EALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNY 677
           EA+IFVFVMHPFDVGDRC +DG+ ++VEEMNILTTVFL+ +N+K+ YPNS+L TKPI+NY
Sbjct: 697 EAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANY 756

Query: 678 YRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKNPQHWRPSHLMVVKEIENVNEIKI 730
           YRSPDM +  EF ++ ATP E+  A++++I  Y++    HW PS ++V +++  +N +KI
Sbjct: 757 YRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKI 816

BLAST of CSPI04G25670 vs. ExPASy TrEMBL
Match: A0A5A7VG97 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001830 PE=3 SV=1)

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 659/736 (89.54%), Postives = 691/736 (93.89%), Query Frame = 0

Query: 1   MDVNGNKP-KVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQN 60
           MDVNGNKP KVVRRS SQKEGENAGQVVV+IS SSVVF KETRD NNYSVL Q+RVDSQN
Sbjct: 1   MDVNGNKPMKVVRRSSSQKEGENAGQVVVDISTSSVVFPKETRDENNYSVLNQNRVDSQN 60

Query: 61  KESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDS 120
           KESTGSSIDHG+DSH PPTAN+PLKIPSSNR   PRRSLKRSILSRPKSRFGEQ R+ DS
Sbjct: 61  KESTGSSIDHGYDSHRPPTANQPLKIPSSNRPPMPRRSLKRSILSRPKSRFGEQPRHIDS 120

Query: 121 DDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVKTVIK 180
           +D FEE HESLREQ GATSSR+S+ NTPKAQPEEEDEEDIVKTEQLNKKHKK KVKT+IK
Sbjct: 121 EDMFEENHESLREQIGATSSRTSAPNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMIK 180

Query: 181 WIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEG 240
           W+VVFC  GCLVASLTVNRLKNCFFLGLE+WKWCLLATVIFCGLI   WA NV VSLIEG
Sbjct: 181 WVVVFCPTGCLVASLTVNRLKNCFFLGLEVWKWCLLATVIFCGLIFIRWATNVAVSLIEG 240

Query: 241 NFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVA 300
           N LLKKKVLYFVHGLKKSVQ TLWLASVLSTWEPLFNQ NHRSSRTTG+ILDAITWTLVA
Sbjct: 241 NCLLKKKVLYFVHGLKKSVQTTLWLASVLSTWEPLFNQSNHRSSRTTGRILDAITWTLVA 300

Query: 301 LLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFS 360
           LLIGSFLWL+KTLLLKILASKFHKDRFFDRIQESIFHHHVLQ LLG PL++  ESAAKFS
Sbjct: 301 LLIGSFLWLIKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLLEGAESAAKFS 360

Query: 361 RCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNV 420
           RCLFS E+ KSD KK+ID GKIH LQREKVS+WTMKVLVEAV+SSAMSISQILDESYYNV
Sbjct: 361 RCLFSLESNKSDHKKLIDMGKIHQLQREKVSAWTMKVLVEAVSSSAMSISQILDESYYNV 420

Query: 421 DDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRI 480
            DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDL +F+VKEEID VLPHFEVDET+RI
Sbjct: 421 GDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLQKFLVKEEIDFVLPHFEVDETRRI 480

Query: 481 GKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKV 540
           GKKALKKWVVKV+QERKTLAHALKDTKTAVKQLNNLVTAVVIIVM VIWLLLMEIATSKV
Sbjct: 481 GKKALKKWVVKVYQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMTVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG+ LLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKN 660
           LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSI+FATPLERIGAMKEKIK YLEKN
Sbjct: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSISFATPLERIGAMKEKIKSYLEKN 660

Query: 661 PQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKIN 720
           PQHWRP+HL+VV+EIENVNEIKIAL+STHTMSYQ+YGEK+KRRSELVMELKRIFEEL IN
Sbjct: 661 PQHWRPNHLLVVREIENVNEIKIALFSTHTMSYQEYGEKLKRRSELVMELKRIFEELNIN 720

Query: 721 YTLLPQTIHLFPVESH 736
           Y L PQTIHLFPVE+H
Sbjct: 721 YNLPPQTIHLFPVEAH 736

BLAST of CSPI04G25670 vs. ExPASy TrEMBL
Match: A0A1S3BU27 (mechanosensitive ion channel protein 10-like OS=Cucumis melo OX=3656 GN=LOC103493692 PE=3 SV=1)

HSP 1 Score: 1107.0 bits (2862), Expect = 0.0e+00
Identity = 586/652 (89.88%), Postives = 611/652 (93.71%), Query Frame = 0

Query: 1   MDVNGNKP-KVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQN 60
           MDVNGNKP KVVRRS SQKEGENAGQVVV+IS SSVVF KETRD NNYSVL Q+RVDSQN
Sbjct: 1   MDVNGNKPMKVVRRSSSQKEGENAGQVVVDISTSSVVFPKETRDENNYSVLNQNRVDSQN 60

Query: 61  KESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDS 120
           KESTGSSIDHG+DSH PPTAN+PLKIPSSNR   PRRSLKRSILSRPKSRFGEQ R+ DS
Sbjct: 61  KESTGSSIDHGYDSHRPPTANQPLKIPSSNRPPMPRRSLKRSILSRPKSRFGEQPRHIDS 120

Query: 121 DDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVKTVIK 180
           +D FEE HESLREQ GATSSR+S+ NTPKAQPEEEDEEDIVKTEQLNKKHKK KVKT+IK
Sbjct: 121 EDMFEENHESLREQIGATSSRTSAPNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMIK 180

Query: 181 WIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEG 240
           W+VVFC  GCLVASLTVNRLKNCFFLGLE+WKWCLLATVIFCGLI   WA NV VSLIEG
Sbjct: 181 WVVVFCPTGCLVASLTVNRLKNCFFLGLEVWKWCLLATVIFCGLIFIRWATNVAVSLIEG 240

Query: 241 NFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVA 300
           N LLKKKVLYFVHGLKKSVQ TLWLASVLSTWEPLFNQ NHRSSRTTG+ILDAITWTLVA
Sbjct: 241 NCLLKKKVLYFVHGLKKSVQTTLWLASVLSTWEPLFNQSNHRSSRTTGRILDAITWTLVA 300

Query: 301 LLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFS 360
           LLIGSFLWL+KTLLLKILASKFHKDRFFDRIQESIFHHHVLQ LLG PL++  ESAAKFS
Sbjct: 301 LLIGSFLWLIKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLLEGAESAAKFS 360

Query: 361 RCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNV 420
           RCLFS E+ KSD KK+ID GKIH LQREKVS+WTMKVLVEAV+SSAMSISQILDESYYNV
Sbjct: 361 RCLFSLESNKSDHKKLIDMGKIHQLQREKVSAWTMKVLVEAVSSSAMSISQILDESYYNV 420

Query: 421 DDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRI 480
            DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDL +F+VKEEID VLPHFEVDET+RI
Sbjct: 421 GDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLQKFLVKEEIDFVLPHFEVDETRRI 480

Query: 481 GKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKV 540
           GKKALKKWVVKV+QERKTLAHALKDTKTAVKQLNNLVTAVVIIVM VIWLLLMEIATSKV
Sbjct: 481 GKKALKKWVVKVYQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMTVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG+ LLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEK 652
           LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSI+FATPLERIGAMKEK
Sbjct: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSISFATPLERIGAMKEK 652

BLAST of CSPI04G25670 vs. ExPASy TrEMBL
Match: A0A1S4DZ48 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=3 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 1.5e-290
Identity = 542/738 (73.44%), Postives = 617/738 (83.60%), Query Frame = 0

Query: 1   MDVNGNKPKVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQNK 60
           MDVNGNKP  +RRS SQKE EN G+VVV++S             N YSV KQ+RVDSQ K
Sbjct: 1   MDVNGNKP--IRRSSSQKESENGGEVVVQLSGVE----------NEYSVPKQNRVDSQTK 60

Query: 61  ESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSD 120
           E TGSS+ +G  S + PTAN+P KIP SN TLTPRRSL+RS LS+PKSRFGEQ  Y DSD
Sbjct: 61  EPTGSSVGYGNASQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSD 120

Query: 121 DKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVKTVIKW 180
              EE H SLREQ GATSSRSS+LNTPKAQPE ED  ++     LN+KHKK KVKTV KW
Sbjct: 121 MFEEENHVSLREQIGATSSRSSNLNTPKAQPEGEDGRNM----GLNEKHKKAKVKTVFKW 180

Query: 181 IVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGN 240
           I VFC+I CLVASLTV  LKN F  GL++WKWCLLATVIFCGLI T W MNVVV LIE N
Sbjct: 181 IGVFCIISCLVASLTVKPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERN 240

Query: 241 FLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNH--RSSRTTGKILDAITWTLV 300
           FLLKKKVLYFVHGLKKSVQVTLWL+ VL+TW  LF++RNH   SSR T KILDA+TWTL 
Sbjct: 241 FLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLA 300

Query: 301 ALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKF 360
           +LLIG+FLWL+KTLLLKILASKFH +RFFDRIQES+F HHVLQ LL PP +++ ES AKF
Sbjct: 301 SLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVED-ESTAKF 360

Query: 361 SRCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQIL-DESYY 420
             C F +E+K+SD KK+ID GKIH L+REKVS+WTMKVLV+AVTSS MSISQIL DESY 
Sbjct: 361 RCCRFCFESKESDRKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYR 420

Query: 421 NVDDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETK 480
           +V DG+I +EM +A   A +I +NVALPG KFI+E DLL F++ EE++LV PHFEVD+T+
Sbjct: 421 DVADGDITNEMNVAKEAAKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKTR 480

Query: 481 RIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATS 540
           +I  KAL  WVVKV+Q RKTLAHALKDTKTAVKQLNNL+TA++IIV A+IWLLLMEIAT+
Sbjct: 481 KIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIATT 540

Query: 541 KVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTT 600
           KVLVFLL+QLAVAAFMFGN CKT FEALIFVFVMHPFDVGDRCVVDG+ LLVEEMNILTT
Sbjct: 541 KVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTT 600

Query: 601 VFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLE 660
           VFLKLNNEKVYYPNSVLATKPI+NYYRSPDM +T EFSI F TP+ERIGAMKE+IKRYLE
Sbjct: 601 VFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLE 660

Query: 661 KNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELK 720
           +NPQHW P+H +VVKEIENVN+IKIALY+ HTM++QD+ EK +RR+ELVMELKRIFEELK
Sbjct: 661 ENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFEELK 720

Query: 721 INYTLLPQTIHLFPVESH 736
           INY LLPQT+HLFPVE H
Sbjct: 721 INYNLLPQTVHLFPVEGH 721

BLAST of CSPI04G25670 vs. ExPASy TrEMBL
Match: A0A5D3D991 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001820 PE=3 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 1.5e-290
Identity = 542/738 (73.44%), Postives = 617/738 (83.60%), Query Frame = 0

Query: 1   MDVNGNKPKVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQNK 60
           MDVNGNKP  +RRS SQKE EN G+VVV++S             N YSV KQ+RVDSQ K
Sbjct: 1   MDVNGNKP--IRRSSSQKESENGGEVVVQLSGVE----------NEYSVPKQNRVDSQTK 60

Query: 61  ESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSD 120
           E TGSS+ +G  S + PTAN+P KIP SN TLTPRRSL+RS LS+PKSRFGEQ  Y DSD
Sbjct: 61  EPTGSSVGYGNASQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSD 120

Query: 121 DKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVKTVIKW 180
              EE H SLREQ GATSSRSS+LNTPKAQPE ED  ++     LN+KHKK KVKTV KW
Sbjct: 121 MFEEENHVSLREQIGATSSRSSNLNTPKAQPEGEDGRNM----GLNEKHKKAKVKTVFKW 180

Query: 181 IVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGN 240
           I VFC+I CLVASLTV  LKN F  GL++WKWCLLATVIFCGLI T W MNVVV LIE N
Sbjct: 181 IGVFCIISCLVASLTVKPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLIERN 240

Query: 241 FLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNH--RSSRTTGKILDAITWTLV 300
           FLLKKKVLYFVHGLKKSVQVTLWL+ VL+TW  LF++RNH   SSR T KILDA+TWTL 
Sbjct: 241 FLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLA 300

Query: 301 ALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKF 360
           +LLIG+FLWL+KTLLLKILASKFH +RFFDRIQES+F HHVLQ LL PP +++ ES AKF
Sbjct: 301 SLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVED-ESTAKF 360

Query: 361 SRCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQIL-DESYY 420
             C F +E+K+SD KK+ID GKIH L+REKVS+WTMKVLV+AVTSS MSISQIL DESY 
Sbjct: 361 RCCRFCFESKESDRKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDESYR 420

Query: 421 NVDDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETK 480
           +V DG+I +EM +A   A +I +NVALPG KFI+E DLL F++ EE++LV PHFEVD+T+
Sbjct: 421 DVADGDITNEMNVAKEAAKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVDKTR 480

Query: 481 RIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATS 540
           +I  KAL  WVVKV+Q RKTLAHALKDTKTAVKQLNNL+TA++IIV A+IWLLLMEIAT+
Sbjct: 481 KIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEIATT 540

Query: 541 KVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTT 600
           KVLVFLL+QLAVAAFMFGN CKT FEALIFVFVMHPFDVGDRCVVDG+ LLVEEMNILTT
Sbjct: 541 KVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTT 600

Query: 601 VFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLE 660
           VFLKLNNEKVYYPNSVLATKPI+NYYRSPDM +T EFSI F TP+ERIGAMKE+IKRYLE
Sbjct: 601 VFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLE 660

Query: 661 KNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELK 720
           +NPQHW P+H +VVKEIENVN+IKIALY+ HTM++QD+ EK +RR+ELVMELKRIFEELK
Sbjct: 661 ENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFEELK 720

Query: 721 INYTLLPQTIHLFPVESH 736
           INY LLPQT+HLFPVE H
Sbjct: 721 INYNLLPQTVHLFPVEGH 721

BLAST of CSPI04G25670 vs. ExPASy TrEMBL
Match: A0A6J1C8Z8 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111009404 PE=3 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 1.1e-285
Identity = 548/752 (72.87%), Postives = 621/752 (82.58%), Query Frame = 0

Query: 1   MDVNGNKP-KVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDR-VDSQ 60
           MDVNGNKP K VRRS SQKE EN GQVVVEI  S VV SKETRD N  SV  Q+R VDSQ
Sbjct: 1   MDVNGNKPLKAVRRSSSQKESENGGQVVVEI--SGVVPSKETRDENGCSVPSQNRVVDSQ 60

Query: 61  NKESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTD 120
           NK  T SSI          +AN+P KIP+S+ TL PR+SLKRSI S+PKSRFGEQ  Y  
Sbjct: 61  NKGPTDSSIG---------SANKPPKIPNSSGTLMPRKSLKRSIQSKPKSRFGEQQPYYI 120

Query: 121 SDDKFEEKHESLREQTGATSSR------SSSLNTPKAQPEEEDEEDIVKTEQLNK-KHKK 180
             D FEE   SLREQ GATSSR       S+L TP+AQ EEEDE+ I KTEQL+K KHKK
Sbjct: 121 DSDMFEEDRLSLREQIGATSSRRASPGHKSALTTPRAQAEEEDEDAIWKTEQLSKEKHKK 180

Query: 181 CKVKTVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMN 240
            K+KT++KW+ VFC+IGCLVASLTVNRL+NCF  GLEIWKWCLLATVI CG+I T W MN
Sbjct: 181 LKMKTLVKWVGVFCIIGCLVASLTVNRLRNCFLWGLEIWKWCLLATVILCGMIFTKWVMN 240

Query: 241 VVVSLIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHR--SSRTTGKI 300
           V+V LIE NFLLKKKVLYFVHGLKK VQVTLWL  VL+TW  LF++ NHR   S+T GKI
Sbjct: 241 VIVFLIERNFLLKKKVLYFVHGLKKIVQVTLWLTLVLATWASLFDRSNHRVLRSKTAGKI 300

Query: 301 LDAITWTLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLM 360
           LDA TWTLV+LLIG+FLWLVKTLLLKILASKFH +RFFDRIQESIFHHHVLQ L  PPLM
Sbjct: 301 LDAGTWTLVSLLIGAFLWLVKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLSRPPLM 360

Query: 361 --QEVESAAKFSRCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMS 420
              E  ++AK S    S + KKSD KK+ID GKIH L+REKVS+WTMKVLV+AVTSS +S
Sbjct: 361 GVAECAASAKSSSGRLSLKGKKSDHKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSELS 420

Query: 421 ISQILDESYYN----VDDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEE 480
           ISQ+LDESY N    V D +I  EME+A   A +I  NVALPG KFI+EEDLL+F++KEE
Sbjct: 421 ISQLLDESYQNGGAEVADSDITDEMEVARAAAYRIFNNVALPGNKFIEEEDLLKFMIKEE 480

Query: 481 IDLVLPHFEVDETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIV 540
           +DLVLP FEV +T++I +KAL  WVVKV+Q RKTLAHALKDTKTAVKQLNNLVTA++IIV
Sbjct: 481 VDLVLPLFEVADTRQIDRKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLVTALLIIV 540

Query: 541 MAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVD 600
            AV+WLLLMEIAT+KVLVFLLSQLAVAAFMFGN CKTTFEALIFVFVMHPFDVGDRCVVD
Sbjct: 541 TAVVWLLLMEIATTKVLVFLLSQLAVAAFMFGNTCKTTFEALIFVFVMHPFDVGDRCVVD 600

Query: 601 GIPLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLE 660
           G+PLLVEEMNILTTVFLKL+NEKVYYPNSVL+TKPI+NYYRSPDMS+T EFSI+F TPLE
Sbjct: 601 GVPLLVEEMNILTTVFLKLSNEKVYYPNSVLSTKPITNYYRSPDMSDTVEFSISFTTPLE 660

Query: 661 RIGAMKEKIKRYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRS 720
           RIGAMKE+IKRYLEKN QHW P+H +VVKEIE+VN+IKIALY  HT+++Q++ EK +RR+
Sbjct: 661 RIGAMKERIKRYLEKNSQHWHPNHDVVVKEIEDVNKIKIALYVNHTLNFQEWAEKNRRRT 720

Query: 721 ELVMELKRIFEELKINYTLLPQTIHLFPVESH 736
           ELVMELK+IFEEL INY LLPQT+HLFPVE+H
Sbjct: 721 ELVMELKKIFEELNINYNLLPQTVHLFPVEAH 741

BLAST of CSPI04G25670 vs. NCBI nr
Match: XP_004141432.2 (mechanosensitive ion channel protein 10 [Cucumis sativus])

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 732/735 (99.59%), Postives = 733/735 (99.73%), Query Frame = 0

Query: 1   MDVNGNKPKVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQNK 60
           MDVNGNKPKVVRRS SQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQNK
Sbjct: 1   MDVNGNKPKVVRRSSSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQNK 60

Query: 61  ESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSD 120
           ESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSD
Sbjct: 61  ESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSD 120

Query: 121 DKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVKTVIKW 180
           DKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKK KVKTVIKW
Sbjct: 121 DKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTVIKW 180

Query: 181 IVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGN 240
           IVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGN
Sbjct: 181 IVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGN 240

Query: 241 FLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVAL 300
           FLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVAL
Sbjct: 241 FLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVAL 300

Query: 301 LIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFSR 360
           LIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFSR
Sbjct: 301 LIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFSR 360

Query: 361 CLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNVD 420
           CLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNVD
Sbjct: 361 CLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNVD 420

Query: 421 DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRIG 480
           DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRIG
Sbjct: 421 DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRIG 480

Query: 481 KKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKVL 540
           KKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKVL
Sbjct: 481 KKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKVL 540

Query: 541 VFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTTVFL 600
           VFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG+PLLVEEMNILTTVFL
Sbjct: 541 VFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVPLLVEEMNILTTVFL 600

Query: 601 KLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKNP 660
           KLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKNP
Sbjct: 601 KLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKNP 660

Query: 661 QHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKINY 720
           QHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKINY
Sbjct: 661 QHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKINY 720

Query: 721 TLLPQTIHLFPVESH 736
           TLLPQTIHLFPVESH
Sbjct: 721 TLLPQTIHLFPVESH 735

BLAST of CSPI04G25670 vs. NCBI nr
Match: KAE8649918.1 (hypothetical protein Csa_012502 [Cucumis sativus])

HSP 1 Score: 1391.7 bits (3601), Expect = 0.0e+00
Identity = 728/734 (99.18%), Postives = 731/734 (99.59%), Query Frame = 0

Query: 1   MDVNGNKPKVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQNK 60
           MDVNGNKPKVVRRS SQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQNK
Sbjct: 1   MDVNGNKPKVVRRSSSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQNK 60

Query: 61  ESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSD 120
           ESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSD
Sbjct: 61  ESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSD 120

Query: 121 DKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVKTVIKW 180
           DKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKK KVKTVIKW
Sbjct: 121 DKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTVIKW 180

Query: 181 IVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGN 240
           IVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGN
Sbjct: 181 IVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGN 240

Query: 241 FLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVAL 300
           FLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVAL
Sbjct: 241 FLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVAL 300

Query: 301 LIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFSR 360
           LIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFSR
Sbjct: 301 LIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFSR 360

Query: 361 CLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNVD 420
           CLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNVD
Sbjct: 361 CLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNVD 420

Query: 421 DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRIG 480
           DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRIG
Sbjct: 421 DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRIG 480

Query: 481 KKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKVL 540
           KKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKVL
Sbjct: 481 KKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKVL 540

Query: 541 VFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTTVFL 600
           VFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG+PLLVEEMNILTTVFL
Sbjct: 541 VFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVPLLVEEMNILTTVFL 600

Query: 601 KLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKNP 660
           KLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKNP
Sbjct: 601 KLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKNP 660

Query: 661 QHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKINY 720
           QHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKINY
Sbjct: 661 QHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKINY 720

Query: 721 TLLPQTIHLFPVES 735
           TLLPQTIHLFP ++
Sbjct: 721 TLLPQTIHLFPFQA 734

BLAST of CSPI04G25670 vs. NCBI nr
Match: KAA0064529.1 (mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa] >TYK20061.1 mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 659/736 (89.54%), Postives = 691/736 (93.89%), Query Frame = 0

Query: 1   MDVNGNKP-KVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQN 60
           MDVNGNKP KVVRRS SQKEGENAGQVVV+IS SSVVF KETRD NNYSVL Q+RVDSQN
Sbjct: 1   MDVNGNKPMKVVRRSSSQKEGENAGQVVVDISTSSVVFPKETRDENNYSVLNQNRVDSQN 60

Query: 61  KESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDS 120
           KESTGSSIDHG+DSH PPTAN+PLKIPSSNR   PRRSLKRSILSRPKSRFGEQ R+ DS
Sbjct: 61  KESTGSSIDHGYDSHRPPTANQPLKIPSSNRPPMPRRSLKRSILSRPKSRFGEQPRHIDS 120

Query: 121 DDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVKTVIK 180
           +D FEE HESLREQ GATSSR+S+ NTPKAQPEEEDEEDIVKTEQLNKKHKK KVKT+IK
Sbjct: 121 EDMFEENHESLREQIGATSSRTSAPNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMIK 180

Query: 181 WIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEG 240
           W+VVFC  GCLVASLTVNRLKNCFFLGLE+WKWCLLATVIFCGLI   WA NV VSLIEG
Sbjct: 181 WVVVFCPTGCLVASLTVNRLKNCFFLGLEVWKWCLLATVIFCGLIFIRWATNVAVSLIEG 240

Query: 241 NFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVA 300
           N LLKKKVLYFVHGLKKSVQ TLWLASVLSTWEPLFNQ NHRSSRTTG+ILDAITWTLVA
Sbjct: 241 NCLLKKKVLYFVHGLKKSVQTTLWLASVLSTWEPLFNQSNHRSSRTTGRILDAITWTLVA 300

Query: 301 LLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFS 360
           LLIGSFLWL+KTLLLKILASKFHKDRFFDRIQESIFHHHVLQ LLG PL++  ESAAKFS
Sbjct: 301 LLIGSFLWLIKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLLEGAESAAKFS 360

Query: 361 RCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNV 420
           RCLFS E+ KSD KK+ID GKIH LQREKVS+WTMKVLVEAV+SSAMSISQILDESYYNV
Sbjct: 361 RCLFSLESNKSDHKKLIDMGKIHQLQREKVSAWTMKVLVEAVSSSAMSISQILDESYYNV 420

Query: 421 DDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRI 480
            DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDL +F+VKEEID VLPHFEVDET+RI
Sbjct: 421 GDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLQKFLVKEEIDFVLPHFEVDETRRI 480

Query: 481 GKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKV 540
           GKKALKKWVVKV+QERKTLAHALKDTKTAVKQLNNLVTAVVIIVM VIWLLLMEIATSKV
Sbjct: 481 GKKALKKWVVKVYQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMTVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG+ LLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKN 660
           LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSI+FATPLERIGAMKEKIK YLEKN
Sbjct: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSISFATPLERIGAMKEKIKSYLEKN 660

Query: 661 PQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKIN 720
           PQHWRP+HL+VV+EIENVNEIKIAL+STHTMSYQ+YGEK+KRRSELVMELKRIFEEL IN
Sbjct: 661 PQHWRPNHLLVVREIENVNEIKIALFSTHTMSYQEYGEKLKRRSELVMELKRIFEELNIN 720

Query: 721 YTLLPQTIHLFPVESH 736
           Y L PQTIHLFPVE+H
Sbjct: 721 YNLPPQTIHLFPVEAH 736

BLAST of CSPI04G25670 vs. NCBI nr
Match: XP_008452774.1 (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo])

HSP 1 Score: 1107.0 bits (2862), Expect = 0.0e+00
Identity = 586/652 (89.88%), Postives = 611/652 (93.71%), Query Frame = 0

Query: 1   MDVNGNKP-KVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQN 60
           MDVNGNKP KVVRRS SQKEGENAGQVVV+IS SSVVF KETRD NNYSVL Q+RVDSQN
Sbjct: 1   MDVNGNKPMKVVRRSSSQKEGENAGQVVVDISTSSVVFPKETRDENNYSVLNQNRVDSQN 60

Query: 61  KESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDS 120
           KESTGSSIDHG+DSH PPTAN+PLKIPSSNR   PRRSLKRSILSRPKSRFGEQ R+ DS
Sbjct: 61  KESTGSSIDHGYDSHRPPTANQPLKIPSSNRPPMPRRSLKRSILSRPKSRFGEQPRHIDS 120

Query: 121 DDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVKTVIK 180
           +D FEE HESLREQ GATSSR+S+ NTPKAQPEEEDEEDIVKTEQLNKKHKK KVKT+IK
Sbjct: 121 EDMFEENHESLREQIGATSSRTSAPNTPKAQPEEEDEEDIVKTEQLNKKHKKWKVKTMIK 180

Query: 181 WIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEG 240
           W+VVFC  GCLVASLTVNRLKNCFFLGLE+WKWCLLATVIFCGLI   WA NV VSLIEG
Sbjct: 181 WVVVFCPTGCLVASLTVNRLKNCFFLGLEVWKWCLLATVIFCGLIFIRWATNVAVSLIEG 240

Query: 241 NFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVA 300
           N LLKKKVLYFVHGLKKSVQ TLWLASVLSTWEPLFNQ NHRSSRTTG+ILDAITWTLVA
Sbjct: 241 NCLLKKKVLYFVHGLKKSVQTTLWLASVLSTWEPLFNQSNHRSSRTTGRILDAITWTLVA 300

Query: 301 LLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFS 360
           LLIGSFLWL+KTLLLKILASKFHKDRFFDRIQESIFHHHVLQ LLG PL++  ESAAKFS
Sbjct: 301 LLIGSFLWLIKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLLEGAESAAKFS 360

Query: 361 RCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNV 420
           RCLFS E+ KSD KK+ID GKIH LQREKVS+WTMKVLVEAV+SSAMSISQILDESYYNV
Sbjct: 361 RCLFSLESNKSDHKKLIDMGKIHQLQREKVSAWTMKVLVEAVSSSAMSISQILDESYYNV 420

Query: 421 DDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRI 480
            DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDL +F+VKEEID VLPHFEVDET+RI
Sbjct: 421 GDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLQKFLVKEEIDFVLPHFEVDETRRI 480

Query: 481 GKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKV 540
           GKKALKKWVVKV+QERKTLAHALKDTKTAVKQLNNLVTAVVIIVM VIWLLLMEIATSKV
Sbjct: 481 GKKALKKWVVKVYQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMTVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG+ LLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEK 652
           LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSI+FATPLERIGAMKEK
Sbjct: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSISFATPLERIGAMKEK 652

BLAST of CSPI04G25670 vs. NCBI nr
Match: XP_038897085.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 1063.9 bits (2750), Expect = 6.2e-307
Identity = 580/734 (79.02%), Postives = 631/734 (85.97%), Query Frame = 0

Query: 1   MDVNGNKP-KVVRRSLSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQN 60
           MDVNGN P K VRRS S KE EN G VVVEI  SSV+ SKET+D N YSV KQ+RVDSQ 
Sbjct: 1   MDVNGNNPSKAVRRSSSLKESENEGHVVVEI--SSVLSSKETKDENGYSVTKQNRVDSQT 60

Query: 61  KESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDS 120
           KE T SSI H  DS++PP ANEPLKIP SN TLT RRSLKRS LSRPKSRFGEQ RYTDS
Sbjct: 61  KEPTDSSIGHSNDSYLPPIANEPLKIPYSNGTLTLRRSLKRSYLSRPKSRFGEQPRYTDS 120

Query: 121 DDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKHKKCKVKTVIK 180
            D FEE   SLREQ GATSSRSS LN P AQ EEED EDIVKTE+ NKK KK KVKT+IK
Sbjct: 121 -DMFEENDVSLREQIGATSSRSSVLNPPNAQSEEEDGEDIVKTEKSNKKDKKVKVKTLIK 180

Query: 181 WIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEG 240
            +  FCLI CLVASLTVNRLKNCFF GL++WKWCLLATVIFCG I T W M+VVV+LIE 
Sbjct: 181 LVGAFCLIACLVASLTVNRLKNCFFWGLKVWKWCLLATVIFCGKIFTTWVMHVVVNLIER 240

Query: 241 NFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVA 300
           NFLLKK VLYFVHGL+KSV+VTLWL  VL TW  LFN  NHRSSRT GKILDAITWTLVA
Sbjct: 241 NFLLKKNVLYFVHGLRKSVRVTLWLTLVLVTWASLFNWNNHRSSRTVGKILDAITWTLVA 300

Query: 301 LLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESAAKFS 360
           LLIG+ LWLVKTLLLKILASKFHK+RFFDRIQESIFHHHVLQ LL  PL +  E  AKFS
Sbjct: 301 LLIGAVLWLVKTLLLKILASKFHKNRFFDRIQESIFHHHVLQTLLKCPLKEGAEGTAKFS 360

Query: 361 RCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQILDESYYNV 420
            C FS ++KKSD KK+IDTGKIH LQREKVS+WT+KVL+EAVTSS MSISQILDES  NV
Sbjct: 361 CCRFSLKSKKSDHKKVIDTGKIHQLQREKVSAWTIKVLIEAVTSSQMSISQILDES-SNV 420

Query: 421 DDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRI 480
            DGEI  EMEIA  VA KI +N+A  GKKFIQEEDLL+F+VKEEIDLVLPHFEVDET++I
Sbjct: 421 ADGEITDEMEIAIAVACKIFKNIA-HGKKFIQEEDLLKFMVKEEIDLVLPHFEVDETRKI 480

Query: 481 GKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKV 540
            KKAL  WVVKV+QERKTLAHAL DTKTAV++LNNL  A++IIV AVIWLLLMEIATSKV
Sbjct: 481 DKKALINWVVKVYQERKTLAHALNDTKTAVEELNNLAIALIIIVTAVIWLLLMEIATSKV 540

Query: 541 LVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMNILTTVF 600
           LVFLLSQLAVAAFMFGNACKT FEALIFVFVMHPFDVGD CVVDG+ LLVEEMNILTTVF
Sbjct: 541 LVFLLSQLAVAAFMFGNACKTAFEALIFVFVMHPFDVGDFCVVDGVQLLVEEMNILTTVF 600

Query: 601 LKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKN 660
           LKLNNEKVYYPNSVLATKPI+NYYRSP+M +T EFSI+FATPLERIGAMKEKIKRYLEKN
Sbjct: 601 LKLNNEKVYYPNSVLATKPITNYYRSPNMFDTIEFSISFATPLERIGAMKEKIKRYLEKN 660

Query: 661 PQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKIN 720
           PQHW P+H +VVKEIENVN+IKIAL++ HTM++QD+ EK +RR+ELVMELKRIFEELKIN
Sbjct: 661 PQHWHPNHSVVVKEIENVNKIKIALFAKHTMNFQDWSEKNRRRTELVMELKRIFEELKIN 720

Query: 721 YTLLPQTIHLFPVE 734
           Y LLPQT+HLFPV+
Sbjct: 721 YNLLPQTVHLFPVQ 729

BLAST of CSPI04G25670 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.4e-179
Identity = 363/685 (52.99%), Postives = 485/685 (70.80%), Query Frame = 0

Query: 73  SHIPPTANEPLKIPSSNRT-LTPRRSLKRSILSRPKSRFGEQSRYTDSDDKFEEKHESLR 132
           S +  + N+P + P+ N   LT R+S  RS+ S+PKSRF + S   D+    EE    +R
Sbjct: 52  SKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VR 111

Query: 133 EQTGA--TSSRSSSLN------------TP-KAQPEEEDEEDIVKTEQLNKK-HKKCKVK 192
           EQ GA  + SR+S  N            TP K   E++++E+I K  +LN++   K    
Sbjct: 112 EQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTL 171

Query: 193 TVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVS 252
            +I+      ++  LVASLT+N LK+  F GLE+WKWC+L  VIF G+++T+W M ++V 
Sbjct: 172 ALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVF 231

Query: 253 LIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITW 312
           LIE NFLL++KVLYFVHGLKKSVQV +WL  +L  W  LFN    RS   T K+L  IT 
Sbjct: 232 LIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAAT-KVLKCITR 291

Query: 313 TLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESA 372
           TL+++L G+F WLVKTLLLKILA+ F+ + FFDRIQ+S+FH +VLQ L G PLM+E E  
Sbjct: 292 TLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERV 351

Query: 373 AK---FSRCLFSWENKKSDL--KKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMS-IS 432
            +        F+   KK  +  KK+ID GK+H ++REKVS+WTM+VL+EAV +S +S IS
Sbjct: 352 GREPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTIS 411

Query: 433 QILDESYYNVD----DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEID 492
             LDE+ Y       D EI  EME A   A  + RNVA P   +I+EEDLL+F++KEE+D
Sbjct: 412 DTLDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVD 471

Query: 493 LVLPHFE-VDETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVM 552
           LV P F+   ET RI +KA  +WVVKV+  R+ LAH+L DTKTAVKQLN LVTA++++V 
Sbjct: 472 LVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVT 531

Query: 553 AVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG 612
            VIWLLL+E+AT+KVL+F  +QL   AF+ G+ CK  FE+++FVFVMHP+DVGDRCVVDG
Sbjct: 532 VVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDG 591

Query: 613 IPLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLER 672
           + +LVEEMN+LTTVFLKLNNEKVYYPN+VLATKPISNY+RSP+M ET EFSI+F+TP+ +
Sbjct: 592 VAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSK 651

Query: 673 IGAMKEKIKRYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSE 730
           I  +KE+I  YLE+NPQHW P H +VVKEIEN+N++K+ALYS HT+++Q+  E+  RR+E
Sbjct: 652 IAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTE 711

BLAST of CSPI04G25670 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.4e-179
Identity = 363/685 (52.99%), Postives = 485/685 (70.80%), Query Frame = 0

Query: 73  SHIPPTANEPLKIPSSNRT-LTPRRSLKRSILSRPKSRFGEQSRYTDSDDKFEEKHESLR 132
           S +  + N+P + P+ N   LT R+S  RS+ S+PKSRF + S   D+    EE    +R
Sbjct: 52  SKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VR 111

Query: 133 EQTGA--TSSRSSSLN------------TP-KAQPEEEDEEDIVKTEQLNKK-HKKCKVK 192
           EQ GA  + SR+S  N            TP K   E++++E+I K  +LN++   K    
Sbjct: 112 EQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTL 171

Query: 193 TVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVS 252
            +I+      ++  LVASLT+N LK+  F GLE+WKWC+L  VIF G+++T+W M ++V 
Sbjct: 172 ALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVF 231

Query: 253 LIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITW 312
           LIE NFLL++KVLYFVHGLKKSVQV +WL  +L  W  LFN    RS   T K+L  IT 
Sbjct: 232 LIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAAT-KVLKCITR 291

Query: 313 TLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESA 372
           TL+++L G+F WLVKTLLLKILA+ F+ + FFDRIQ+S+FH +VLQ L G PLM+E E  
Sbjct: 292 TLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERV 351

Query: 373 AK---FSRCLFSWENKKSDL--KKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMS-IS 432
            +        F+   KK  +  KK+ID GK+H ++REKVS+WTM+VL+EAV +S +S IS
Sbjct: 352 GREPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTIS 411

Query: 433 QILDESYYNVD----DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEID 492
             LDE+ Y       D EI  EME A   A  + RNVA P   +I+EEDLL+F++KEE+D
Sbjct: 412 DTLDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVD 471

Query: 493 LVLPHFE-VDETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVM 552
           LV P F+   ET RI +KA  +WVVKV+  R+ LAH+L DTKTAVKQLN LVTA++++V 
Sbjct: 472 LVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVT 531

Query: 553 AVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG 612
            VIWLLL+E+AT+KVL+F  +QL   AF+ G+ CK  FE+++FVFVMHP+DVGDRCVVDG
Sbjct: 532 VVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDG 591

Query: 613 IPLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLER 672
           + +LVEEMN+LTTVFLKLNNEKVYYPN+VLATKPISNY+RSP+M ET EFSI+F+TP+ +
Sbjct: 592 VAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSK 651

Query: 673 IGAMKEKIKRYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSE 730
           I  +KE+I  YLE+NPQHW P H +VVKEIEN+N++K+ALYS HT+++Q+  E+  RR+E
Sbjct: 652 IAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTE 711

BLAST of CSPI04G25670 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.4e-179
Identity = 363/685 (52.99%), Postives = 485/685 (70.80%), Query Frame = 0

Query: 73  SHIPPTANEPLKIPSSNRT-LTPRRSLKRSILSRPKSRFGEQSRYTDSDDKFEEKHESLR 132
           S +  + N+P + P+ N   LT R+S  RS+ S+PKSRF + S   D+    EE    +R
Sbjct: 52  SKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE----VR 111

Query: 133 EQTGA--TSSRSSSLN------------TP-KAQPEEEDEEDIVKTEQLNKK-HKKCKVK 192
           EQ GA  + SR+S  N            TP K   E++++E+I K  +LN++   K    
Sbjct: 112 EQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMRSKISTL 171

Query: 193 TVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVS 252
            +I+      ++  LVASLT+N LK+  F GLE+WKWC+L  VIF G+++T+W M ++V 
Sbjct: 172 ALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVF 231

Query: 253 LIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITW 312
           LIE NFLL++KVLYFVHGLKKSVQV +WL  +L  W  LFN    RS   T K+L  IT 
Sbjct: 232 LIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAAT-KVLKCITR 291

Query: 313 TLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVESA 372
           TL+++L G+F WLVKTLLLKILA+ F+ + FFDRIQ+S+FH +VLQ L G PLM+E E  
Sbjct: 292 TLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERV 351

Query: 373 AK---FSRCLFSWENKKSDL--KKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMS-IS 432
            +        F+   KK  +  KK+ID GK+H ++REKVS+WTM+VL+EAV +S +S IS
Sbjct: 352 GREPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTIS 411

Query: 433 QILDESYYNVD----DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEID 492
             LDE+ Y       D EI  EME A   A  + RNVA P   +I+EEDLL+F++KEE+D
Sbjct: 412 DTLDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVD 471

Query: 493 LVLPHFE-VDETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVM 552
           LV P F+   ET RI +KA  +WVVKV+  R+ LAH+L DTKTAVKQLN LVTA++++V 
Sbjct: 472 LVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVT 531

Query: 553 AVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDG 612
            VIWLLL+E+AT+KVL+F  +QL   AF+ G+ CK  FE+++FVFVMHP+DVGDRCVVDG
Sbjct: 532 VVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDG 591

Query: 613 IPLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLER 672
           + +LVEEMN+LTTVFLKLNNEKVYYPN+VLATKPISNY+RSP+M ET EFSI+F+TP+ +
Sbjct: 592 VAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSK 651

Query: 673 IGAMKEKIKRYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSE 730
           I  +KE+I  YLE+NPQHW P H +VVKEIEN+N++K+ALYS HT+++Q+  E+  RR+E
Sbjct: 652 IAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTE 711

BLAST of CSPI04G25670 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 563.5 bits (1451), Expect = 2.4e-160
Identity = 334/747 (44.71%), Postives = 488/747 (65.33%), Query Frame = 0

Query: 15  LSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDS---QNKESTGSSIDHGF 74
           ++++   N  +VV+ +S+     SK+ R   +++ L     D+   ++K     SI    
Sbjct: 1   MAERRVSNGEEVVINVSDKE--DSKDPRASPSFNPLASPDSDAGIEKSKPVPPISIPTPE 60

Query: 75  DSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDSDDKFEEKHESLR 134
                 + ++P KIPS    L  R+SL RSI S+PKSRFGEQ  +     + E    SLR
Sbjct: 61  IYKFSGSVHKPPKIPSP-EGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLR 120

Query: 135 EQTGATS-----------------SRSSSLNTPKAQPEEEDEEDIVKTEQLNK-KHKKCK 194
           EQ GA S                 S +S+  +  A+ E ++ E+I K  +L++ K    K
Sbjct: 121 EQFGAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRSGMK 180

Query: 195 VKTVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVV 254
               ++ +V   ++G L+ SLT++ +      GLE WKWC+L  V   G+++T+W M+ V
Sbjct: 181 PLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFV 240

Query: 255 VSLIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAI 314
           V +IE N+LL+KKVLYFVHGLKK+VQV +W + VL  W  LF+  + + +R T + LD I
Sbjct: 241 VFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFD-GDVKRTRKTKRFLDFI 300

Query: 315 TWTLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQEVE 374
           TWT+V+LL+GS L+LVKT  LK+LASKF+   FF+RIQES+FH +VLQ L GPPL++E E
Sbjct: 301 TWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEEAE 360

Query: 375 SAAK--------FSRCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAV-TSS 434
           +  +        F+R   + + K  D KK+ID GK+H +++EKVS+WTM+VL+EAV TS 
Sbjct: 361 NVGRVPSTGHLSFTR---TKDGKVKD-KKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSG 420

Query: 435 AMSISQILDESYYNVD--DGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKE 494
             +IS  LDE     +  D EI +EME A   A  +  NVA P   +I+E+DLL+F++KE
Sbjct: 421 ISTISSTLDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKE 480

Query: 495 EIDLVLPHFEVDETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVII 554
           E+DLVLP  E  +T +I +K   +WVV V+  RKT+ H+L DTKTAVKQL+ L+T ++ +
Sbjct: 481 EVDLVLPLIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILTV 540

Query: 555 VMAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVV 614
           +  ++W++L++IA++K+L+   SQ    AFM G+ CK  FE+ +FVFVMHP+DVGDRCVV
Sbjct: 541 ITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCVV 600

Query: 615 DGIPLLVEEMNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPL 674
           DG+ LLVEE+++LTTVFLK++NEKV+YPNSVL +KPISN+YRSPDM +  +F I F+TP 
Sbjct: 601 DGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTPA 660

Query: 675 ERIGAMKEKIKRYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRR 730
           E+IG +K KI  YL  N QHW P   ++V+ IEN+N++ + +   HT+++Q Y EK  RR
Sbjct: 661 EKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLRR 720

BLAST of CSPI04G25670 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 441.4 bits (1134), Expect = 1.4e-123
Identity = 269/676 (39.79%), Postives = 405/676 (59.91%), Query Frame = 0

Query: 99  KRSILSRPKSRFGEQSRYTDSDDKFEEKHESLREQTGATSS----RSSSLNTPKAQP-EE 158
           K+ +LSR K++   Q   T +    ++       ++G   S    +S    TP     EE
Sbjct: 185 KKPMLSRNKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEE 244

Query: 159 EDEEDIVKTEQLNKKHKKCKVK--TVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWK 218
           E+EED    E L ++ K+ K+     ++WI +  ++  LV SLT++ L+   +  L++WK
Sbjct: 245 EEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWK 304

Query: 219 WCLLATVIFCGLILTHWAMNVVVSLIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTW 278
           W +   V+ CG +++ W + ++V L+E NF  +K+VLYFV+G++KSVQ  LWL  VL  W
Sbjct: 305 WEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAW 364

Query: 279 EPLFNQRNHRSSRTTGKILDAITWTLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQ 338
             LF+++  R +R+T   L  +T  LV LL+   +WLVKT+L+K+LAS FH   +FDRIQ
Sbjct: 365 HFLFDKKVERETRSTA--LRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQ 424

Query: 339 ESIFHHHVLQALLGPPLM-------------QEVESAAKFSRCLFSWENKKSDLKKIIDT 398
           ES+F  +V++ L GPPLM             ++V+S  K +         K+ +K  +  
Sbjct: 425 ESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLP-PALKATVKSFMKV 484

Query: 399 GKIHHLQR----------------------EKVSSWTMKVLVEAVTSSAMSI--SQILDE 458
           GK   L R                      + VS+W MK L+  +   A+S     + D 
Sbjct: 485 GKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDT 544

Query: 459 SYYNVDDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFE-V 518
           +  + D   I  E E A   A KI  NV  PG ++I  ED L+F+ +EE +  +  FE  
Sbjct: 545 TQEDEDATHIRSEYE-AKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGA 604

Query: 519 DETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLME 578
            E+ +I K  LK WVVK F+ER+ LA  L DTKTAV +L+ ++  V+ I++ +IWLL++ 
Sbjct: 605 SESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILG 664

Query: 579 IATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGIPLLVEEMN 638
           IAT++ L+ L SQL + AF+FGN+CKT FEA+IF+FVMHPFDVGDRC +DG+ L+VEEMN
Sbjct: 665 IATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMN 724

Query: 639 ILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIK 698
           ILTTVFL+ +N+K+ YPNSVL TKPI+NYYRSPDM +  EF ++ ATP E+I A+K++I 
Sbjct: 725 ILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRIL 784

Query: 699 RYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIF 730
            Y++    +W P+ ++V   ++++N +KIA++ TH M++QD GE+  RR  L+ E+ +  
Sbjct: 785 SYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTC 844

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYG91.9e-17852.99Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10... [more]
Q84M973.4e-15944.71Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 P... [more]
Q9LH742.0e-12239.79Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
Q9SYM19.8e-12239.41Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Q9LPG31.8e-12040.03Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
Match NameE-valueIdentityDescription
A0A5A7VG970.0e+0089.54Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BU270.0e+0089.88mechanosensitive ion channel protein 10-like OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A1S4DZ481.5e-29073.44Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=... [more]
A0A5D3D9911.5e-29073.44Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1C8Z81.1e-28572.87Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111009... [more]
Match NameE-valueIdentityDescription
XP_004141432.20.0e+0099.59mechanosensitive ion channel protein 10 [Cucumis sativus][more]
KAE8649918.10.0e+0099.18hypothetical protein Csa_012502 [Cucumis sativus][more]
KAA0064529.10.0e+0089.54mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa] >TYK2006... [more]
XP_008452774.10.0e+0089.88PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo][more]
XP_038897085.16.2e-30779.02mechanosensitive ion channel protein 10-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G12080.11.4e-17952.99mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.21.4e-17952.99mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.31.4e-17952.99mechanosensitive channel of small conductance-like 10 [more]
AT5G19520.12.4e-16044.71mechanosensitive channel of small conductance-like 9 [more]
AT3G14810.11.4e-12339.79mechanosensitive channel of small conductance-like 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPIRSFPIRSF017209Memb_At2g17000coord: 16..734
e-value: 2.9E-191
score: 635.9
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 24..729
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 514..719
e-value: 3.3E-21
score: 75.8
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 573..622
e-value: 1.7E-10
score: 42.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..98
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..159
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 111..133
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..148
NoneNo IPR availablePANTHERPTHR31618:SF7MECHANOSENSITIVE ION CHANNEL PROTEINcoord: 24..729
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 562..622

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G25670.1CSPI04G25670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0050982 detection of mechanical stimulus
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008381 mechanosensitive ion channel activity