CSPI04G24390 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G24390
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionB30.2/SPRY domain-containing protein
LocationChr4: 22172135 .. 22174902 (-)
RNA-Seq ExpressionCSPI04G24390
SyntenyCSPI04G24390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCCACTCTAAATTTTATTATTAATTGAAAATTACTTATATAAATTTCCCATTTTTCAAAAAGAATTCCTGTTTATGGTAATAACTGAAGTGTCTGAGGTAACATGAACAAATGTTTATGGTGTTTTGTGTTAGTTTGTTCTTTAACATCCGCCGTGTACGGCGGAGTGAATCAGCGCATTTCATTTTTCTATCAGCAGAACTTTGTCGGCTAATTTGTATTAAATAAATCTTTGAGCTCTGTCTATTCCTTTCAACTGCTTCAACTTCAAGCCATAAGATGAGACGACTATTAACTTTCTTCTTGTTTGGAAAAAGTCTTTAGTCCACGCATGCCTTATTTTCTGCCGCCCCCATTTGCTTGTTTCTTATATTAACTTCCTCCGCCATTCTCTCTTGAACTCTATTTCTCCTCGAAAGTTTCTTCCTTTTTGTCCATTCTATGTAAAATCACCCGTCTTAAAAATGGTGGGAACCTCTGTTGTTGCTGTAGCTGCCGTGTGTATTGCAGTTCTTGCATTGTTACTTACTGGACTGTACTTTTGGAAGCGGCGACGGTGGGGTATTGTTGAATCGGAAACTATTGGGAAGTTACAAAGTGTGGAAAGTTCTCAACAGAGATCAGCTTCTGGGGCTTTGAAGCTTCATCACCAATCTGAATCAGAGGGAAAAAGGCGACTCAGTAATTTTTACCCTCGTGGGGTTTCTCAAAAGCCTTTGTTCAGTTGGGACGACAGCCCTTCGCTCGTTAACGATGCGGTTGAAAATGGATGGACTCAATTCGCTTTTACGGACTACGTGTCGTCTTCTCCAACTTCAAGATCCCGATTGTTGGGTTTATGTTCGGCCAGTGAGATCGAGAAGGAGATACCTGAGGCGGAGATTAGCTGGGAAGTGAGTCAAGGATCGGCTGATTTTATGCAAAAGATTAGGCTTAATTCTGGGTTTAAGAAGATGATTAACAATACAATCTCTTCTTATCCTGCTTCCTCTGTAATCAAAACCGCTCTCCCATTACCGGGACCTCCCCTGGCTTCCTTCCCGCAAGAAGCGTATTTTGAAATCACGATTCTGAATATTTACGGCGATGAAAATGAACCCACCGGAACGGCGAAAGAAGGGGAGAGAATCAAACTGATACCTGAGAATCATAGCTCGAAGGCAAGTTCAGAGTCTTTGGCCTATTTTACATCAAATAACAAAGTTAGCAACGTTGAGGAATCAAAGCTCGATGGGAAAGGACAAGAGGATGAAATAGTTGAAGGTGTGATGTTATCCGTTGGGCTAATTTCCGGTGGCTCCGCCCCTTCTAAACTTCCCGGCAGCTATTCGGGTAGCATCGGATTCAACTCCAATGGCTCAGTCTATCTCGACGGTAAAATCCCAAAGAAAAAAAGATCATTCAATTTCAATTATCAAGTACAAATCCAAACCCCCCATTCTTCTTTACTGAAAATAGGAAATCAAAAGAAGAACATTTTTTTCCCTCATGGACACTCTGCTTTAGTGTTTAGCAATTAAAATGTAAAATAAAATAGCATATAAAAAATAAAGAATCGGATTGATTTGTTTAATACCAACCAACATTGACAAAAATAAATGTTTTATTTTTTTTAATTTCGTCATTATTAGGCAAGCTGTATGGGCGATACTACCAAATTAGAACTGATATGCTCTGTTCTAACTCTGTTATTGATTTTCTTTCTTTTCTTTCAGGAATAAAATTAGTGTTTGAATCCGAAAAGGCAGATTGGGGAAGGGCGGAGAAAGTGATCGGATGTGGGTTTGATCCAAAACAAAAGAAAGTGTTCTTCACGGTGGATTCAGAATTAGTGCACGTAATTCATTGCAAGTCGGAGGAATTTGGGAGCCCACTCTATCCGACTCTGGCGGCCAACGGAGACGTTACAGTTTTGGTAAATTTGGGGCAAAGTGTTTTCAAGTATATACCAGCTCAAAGAACTCCAAATCCCTGTTTCGTAAGTCCGCTTGTGAATAATGCCGATGGTTTCCATGGAAATGGGTATGAAGATAGCAGAGAGCTTTTCTCGATGAATATGATCGATTCTCAGTGGTTTAGTCGGCTGACACCGAAACCCAGCAACAATCTTGTTGGTGATCATCGAGAGGACGATGAGTTGTCCAATGATATGGAAAGTTGCGTTGAAATTGAACTTTTTGAAATTGTTGTGGAAAATGAGGAACGGATTGGAAGTAAAACGTAAAAGAATTGGGTTCTGCGTTTTTCTGTTTTAATTAATCTCAGCCAGTTATTTCAAAAAGTGAGATATGGATTGCCAGAAAGAAAGAAGAGAAAATTGTAGTTAGAGGAGAGAACGAATTGAATCGACATACGTTTGTGTTAATTCTTTTGATTATTTGGATTGGGTGAAGGTTTGTTCATATGATTCTCACCTCTCCGTTGTAAATTCGGATTCTCCAGCGGAATGGCAGCGGCAAGATCATCACATTGCCGGCTTCTTTGAGATACGATTTCTATTAATCAATAACTGAAATTGCTTGAATCTTTTGGTGCGTAGTTGACGATTTGTTGCATTCTATATATATATATATATGAACAACAATGATAAAAGAATATGGAAGGTGAAGGGTTTGAATTTGTTGAGGGTACATAGTTATGTAGGTTCAACTATGGTGTTTGTAGTTATACGTTCCTTTAATTATAAGTCAATTCATCCTATTTTATATATCAATACTTAGGGCGAGTGGATCTCTCTCTATTTTTGTACCATCTTTCTAATGGCTAG

mRNA sequence

CTCCCACTCTAAATTTTATTATTAATTGAAAATTACTTATATAAATTTCCCATTTTTCAAAAAGAATTCCTGTTTATGGTAATAACTGAAGTGTCTGAGGTAACATGAACAAATGTTTATGGTGTTTTGTGTTAGTTTGTTCTTTAACATCCGCCGTGTACGGCGGAGTGAATCAGCGCATTTCATTTTTCTATCAGCAGAACTTTGTCGGCTAATTTGTATTAAATAAATCTTTGAGCTCTGTCTATTCCTTTCAACTGCTTCAACTTCAAGCCATAAGATGAGACGACTATTAACTTTCTTCTTGTTTGGAAAAAGTCTTTAGTCCACGCATGCCTTATTTTCTGCCGCCCCCATTTGCTTGTTTCTTATATTAACTTCCTCCGCCATTCTCTCTTGAACTCTATTTCTCCTCGAAAGTTTCTTCCTTTTTGTCCATTCTATGTAAAATCACCCGTCTTAAAAATGGTGGGAACCTCTGTTGTTGCTGTAGCTGCCGTGTGTATTGCAGTTCTTGCATTGTTACTTACTGGACTGTACTTTTGGAAGCGGCGACGGTGGGGTATTGTTGAATCGGAAACTATTGGGAAGTTACAAAGTGTGGAAAGTTCTCAACAGAGATCAGCTTCTGGGGCTTTGAAGCTTCATCACCAATCTGAATCAGAGGGAAAAAGGCGACTCAGTAATTTTTACCCTCGTGGGGTTTCTCAAAAGCCTTTGTTCAGTTGGGACGACAGCCCTTCGCTCGTTAACGATGCGGTTGAAAATGGATGGACTCAATTCGCTTTTACGGACTACGTGTCGTCTTCTCCAACTTCAAGATCCCGATTGTTGGGTTTATGTTCGGCCAGTGAGATCGAGAAGGAGATACCTGAGGCGGAGATTAGCTGGGAAGTGAGTCAAGGATCGGCTGATTTTATGCAAAAGATTAGGCTTAATTCTGGGTTTAAGAAGATGATTAACAATACAATCTCTTCTTATCCTGCTTCCTCTGTAATCAAAACCGCTCTCCCATTACCGGGACCTCCCCTGGCTTCCTTCCCGCAAGAAGCGTATTTTGAAATCACGATTCTGAATATTTACGGCGATGAAAATGAACCCACCGGAACGGCGAAAGAAGGGGAGAGAATCAAACTGATACCTGAGAATCATAGCTCGAAGGCAAGTTCAGAGTCTTTGGCCTATTTTACATCAAATAACAAAGTTAGCAACGTTGAGGAATCAAAGCTCGATGGGAAAGGACAAGAGGATGAAATAGTTGAAGGTGTGATGTTATCCGTTGGGCTAATTTCCGGTGGCTCCGCCCCTTCTAAACTTCCCGGCAGCTATTCGGGTAGCATCGGATTCAACTCCAATGGCTCAGTCTATCTCGACGGAATAAAATTAGTGTTTGAATCCGAAAAGGCAGATTGGGGAAGGGCGGAGAAAGTGATCGGATGTGGGTTTGATCCAAAACAAAAGAAAGTGTTCTTCACGGTGGATTCAGAATTAGTGCACGTAATTCATTGCAAGTCGGAGGAATTTGGGAGCCCACTCTATCCGACTCTGGCGGCCAACGGAGACGTTACAGTTTTGGTAAATTTGGGGCAAAGTGTTTTCAAGTATATACCAGCTCAAAGAACTCCAAATCCCTGTTTCGTAAGTCCGCTTGTGAATAATGCCGATGGTTTCCATGGAAATGGGTATGAAGATAGCAGAGAGCTTTTCTCGATGAATATGATCGATTCTCAGTGGTTTAGTCGGCTGACACCGAAACCCAGCAACAATCTTGTTGGTGATCATCGAGAGGACGATGAGTTGTCCAATGATATGGAAAGTTGCGTTGAAATTGAACTTTTTGAAATTGTTGTGGAAAATGAGGAACGGATTGGAAGTAAAACGTAAAAGAATTGGGTTCTGCGTTTTTCTGTTTTAATTAATCTCAGCCAGTTATTTCAAAAAGTGAGATATGGATTGCCAGAAAGAAAGAAGAGAAAATTGTAGTTAGAGGAGAGAACGAATTGAATCGACATACGTTTGTGTTAATTCTTTTGATTATTTGGATTGGGTGAAGGTTTGTTCATATGATTCTCACCTCTCCGTTGTAAATTCGGATTCTCCAGCGGAATGGCAGCGGCAAGATCATCACATTGCCGGCTTCTTTGAGATACGATTTCTATTAATCAATAACTGAAATTGCTTGAATCTTTTGGTGCGTAGTTGACGATTTGTTGCATTCTATATATATATATATATGAACAACAATGATAAAAGAATATGGAAGGTGAAGGGTTTGAATTTGTTGAGGGTACATAGTTATGTAGGTTCAACTATGGTGTTTGTAGTTATACGTTCCTTTAATTATAAGTCAATTCATCCTATTTTATATATCAATACTTAGGGCGAGTGGATCTCTCTCTATTTTTGTACCATCTTTCTAATGGCTAG

Coding sequence (CDS)

ATGGTGGGAACCTCTGTTGTTGCTGTAGCTGCCGTGTGTATTGCAGTTCTTGCATTGTTACTTACTGGACTGTACTTTTGGAAGCGGCGACGGTGGGGTATTGTTGAATCGGAAACTATTGGGAAGTTACAAAGTGTGGAAAGTTCTCAACAGAGATCAGCTTCTGGGGCTTTGAAGCTTCATCACCAATCTGAATCAGAGGGAAAAAGGCGACTCAGTAATTTTTACCCTCGTGGGGTTTCTCAAAAGCCTTTGTTCAGTTGGGACGACAGCCCTTCGCTCGTTAACGATGCGGTTGAAAATGGATGGACTCAATTCGCTTTTACGGACTACGTGTCGTCTTCTCCAACTTCAAGATCCCGATTGTTGGGTTTATGTTCGGCCAGTGAGATCGAGAAGGAGATACCTGAGGCGGAGATTAGCTGGGAAGTGAGTCAAGGATCGGCTGATTTTATGCAAAAGATTAGGCTTAATTCTGGGTTTAAGAAGATGATTAACAATACAATCTCTTCTTATCCTGCTTCCTCTGTAATCAAAACCGCTCTCCCATTACCGGGACCTCCCCTGGCTTCCTTCCCGCAAGAAGCGTATTTTGAAATCACGATTCTGAATATTTACGGCGATGAAAATGAACCCACCGGAACGGCGAAAGAAGGGGAGAGAATCAAACTGATACCTGAGAATCATAGCTCGAAGGCAAGTTCAGAGTCTTTGGCCTATTTTACATCAAATAACAAAGTTAGCAACGTTGAGGAATCAAAGCTCGATGGGAAAGGACAAGAGGATGAAATAGTTGAAGGTGTGATGTTATCCGTTGGGCTAATTTCCGGTGGCTCCGCCCCTTCTAAACTTCCCGGCAGCTATTCGGGTAGCATCGGATTCAACTCCAATGGCTCAGTCTATCTCGACGGAATAAAATTAGTGTTTGAATCCGAAAAGGCAGATTGGGGAAGGGCGGAGAAAGTGATCGGATGTGGGTTTGATCCAAAACAAAAGAAAGTGTTCTTCACGGTGGATTCAGAATTAGTGCACGTAATTCATTGCAAGTCGGAGGAATTTGGGAGCCCACTCTATCCGACTCTGGCGGCCAACGGAGACGTTACAGTTTTGGTAAATTTGGGGCAAAGTGTTTTCAAGTATATACCAGCTCAAAGAACTCCAAATCCCTGTTTCGTAAGTCCGCTTGTGAATAATGCCGATGGTTTCCATGGAAATGGGTATGAAGATAGCAGAGAGCTTTTCTCGATGAATATGATCGATTCTCAGTGGTTTAGTCGGCTGACACCGAAACCCAGCAACAATCTTGTTGGTGATCATCGAGAGGACGATGAGTTGTCCAATGATATGGAAAGTTGCGTTGAAATTGAACTTTTTGAAATTGTTGTGGAAAATGAGGAACGGATTGGAAGTAAAACGTAA

Protein sequence

MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKLHHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRSRLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKTALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASSESLAYFTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSVYLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPTLAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMIDSQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEERIGSKT*
Homology
BLAST of CSPI04G24390 vs. ExPASy Swiss-Prot
Match: Q96UB6 (Protein ssh4 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=ssh4 PE=3 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 6.3e-05
Identity = 38/121 (31.40%), Postives = 61/121 (50.41%), Query Frame = 0

Query: 266 EGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGS-VYLDGIKLVFESEKADWGRAEKVIG 325
           E  ++S+G+ +      +LPG +  S+ + SNG+  Y           +   G    VIG
Sbjct: 169 ENTLISIGMATKPYPLFRLPGFHKYSVAYLSNGTRRYNQPFNATSYGPQVVQG---DVIG 228

Query: 326 CGFDPKQKKVFFTVD-SELVHVIH-CKSEEFGSPLYPTLAANGDVTVLVNLGQSVFKYIP 384
            G+ P+   +FFT +  +L  V+H  KS+ F    +P++ ANG   V VN GQ+ F +I 
Sbjct: 229 VGYRPRSGTIFFTRNGKKLEDVVHGLKSQNF----FPSIGANGPCIVHVNFGQAGFVFIE 282

BLAST of CSPI04G24390 vs. ExPASy Swiss-Prot
Match: Q4WRW0 (Protein ssh4 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=ssh4 PE=3 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 1.8e-04
Identity = 37/121 (30.58%), Postives = 60/121 (49.59%), Query Frame = 0

Query: 266 EGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSVYLDGIKLVFESEKADWGRAE-KVIG 325
           E  ++S+G+ +      +LPG +  S+ + S G          F +       A+  V+G
Sbjct: 234 ESTLISIGMTTKPYPLFRLPGFHKTSVAYLSTGH---RRYNQPFSASPYGPALAQGDVVG 293

Query: 326 CGFDPKQKKVFFTVD-SELVHVIH-CKSEEFGSPLYPTLAANGDVTVLVNLGQSVFKYIP 384
            G+ P+   +FFT +  +L  V+H  K++ F    +PT+ ANG  TV VN GQ  F +I 
Sbjct: 294 VGYRPRSGTIFFTRNGKKLEDVVHGAKTQNF----FPTVGANGPCTVHVNFGQMGFVFIE 347

BLAST of CSPI04G24390 vs. ExPASy Swiss-Prot
Match: A1CNW8 (Protein ssh4 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=ssh4 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 3.1e-04
Identity = 39/121 (32.23%), Postives = 61/121 (50.41%), Query Frame = 0

Query: 266 EGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSVYLDGIKLVFESEKADWGRAE-KVIG 325
           E  ++SVG+ +      +LPG +  S+ ++S G          F S       ++  VIG
Sbjct: 234 ENTLVSVGMTTKPYPLFRLPGFHKYSVAYSSTGH---RRHNQPFASTPYGPPLSQGDVIG 293

Query: 326 CGFDPKQKKVFFTVD-SELVHVIH-CKSEEFGSPLYPTLAANGDVTVLVNLGQSVFKYIP 384
            G+ P+   +FFT +  +L  V+H  K++ F    +PT+ ANG  TV VN GQ  F +I 
Sbjct: 294 VGYRPRSGTIFFTRNGKKLEDVVHAAKTQNF----FPTVGANGPCTVHVNFGQMGFVFIE 347

BLAST of CSPI04G24390 vs. ExPASy Swiss-Prot
Match: O74497 (SPRY domain-containing protein C285.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC285.10c PE=4 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 4.1e-04
Identity = 35/116 (30.17%), Postives = 58/116 (50.00%), Query Frame = 0

Query: 269 MLSVGLISGGSAPSKLPGSYSGSIGFNSNGSVYLDGIKLVFESEK-ADWGRAEKVIGCGF 328
           ++S+GL +    P +LPG    S  + S+G+   +     F  +  + + +   VIG G+
Sbjct: 181 LVSIGLATKPYPPFRLPGWNFWSTAYVSDGTRRSNS---PFTGKPYSSFYQQGDVIGVGY 240

Query: 329 DPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPTLAANGDVTVLVNLGQSVFKYIPA 384
            PK  ++FFT +      + C        LYPT+ A G  T+ VNLGQ+ + +I A
Sbjct: 241 KPKCNRIFFTRNGRRCAELPCTYRN----LYPTVGAIGPCTLHVNLGQAGYVFIEA 289

BLAST of CSPI04G24390 vs. ExPASy TrEMBL
Match: A0A0A0L2G0 (B30.2/SPRY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G641570 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 9.6e-267
Identity = 470/472 (99.58%), Postives = 471/472 (99.79%), Query Frame = 0

Query: 1   MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL 60
           MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL
Sbjct: 1   MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL 60

Query: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS 120
           HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS
Sbjct: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS 120

Query: 121 RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT 180
           RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT
Sbjct: 121 RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT 180

Query: 181 ALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASSESLAY 240
           ALPLPGPPLASFPQEAYFEITILNIYGDEN+PTGTAKEGERIKLIPENHSSKASSESLAY
Sbjct: 181 ALPLPGPPLASFPQEAYFEITILNIYGDENQPTGTAKEGERIKLIPENHSSKASSESLAY 240

Query: 241 FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300
           FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV
Sbjct: 241 FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300

Query: 301 YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT 360
           YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT
Sbjct: 301 YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT 360

Query: 361 LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID 420
           LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID
Sbjct: 361 LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID 420

Query: 421 SQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEERIGSKT 473
           SQWFSRLTPKPSNNLV DHREDDELSNDMESCVEIELFEIVVENEERIGSKT
Sbjct: 421 SQWFSRLTPKPSNNLVDDHREDDELSNDMESCVEIELFEIVVENEERIGSKT 472

BLAST of CSPI04G24390 vs. ExPASy TrEMBL
Match: A0A5A7V9Y8 (SPla/RYanodine receptor SPRY OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G001160 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.8e-250
Identity = 446/472 (94.49%), Postives = 455/472 (96.40%), Query Frame = 0

Query: 1   MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL 60
           MVGTSVVAVAAV IAVLALLLTGLYFWK+RR GIVESETIGKLQSVESSQQRS SGALKL
Sbjct: 1   MVGTSVVAVAAVSIAVLALLLTGLYFWKQRRGGIVESETIGKLQSVESSQQRSGSGALKL 60

Query: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS 120
           HHQSESEGKRRLSNFYPRGVSQK LFSWDDSPSLVNDAVENGWTQFAFTDY SSSPTSRS
Sbjct: 61  HHQSESEGKRRLSNFYPRGVSQKTLFSWDDSPSLVNDAVENGWTQFAFTDYTSSSPTSRS 120

Query: 121 RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT 180
           RLLGLCSASEIEKEIP+ EISWEVS GSADFMQKIRLNSGFKK+INNTISSYPASSVI+T
Sbjct: 121 RLLGLCSASEIEKEIPDTEISWEVSPGSADFMQKIRLNSGFKKIINNTISSYPASSVIRT 180

Query: 181 ALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASSESLAY 240
           ALPLPGPPLASFPQEAYFEITILNI GDENEPTG AKEGERIKLIPENHSSKASSESLAY
Sbjct: 181 ALPLPGPPLASFPQEAYFEITILNISGDENEPTGAAKEGERIKLIPENHSSKASSESLAY 240

Query: 241 FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300
           FTSNNKVSNVEESKL+ KGQEDEIVE +MLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV
Sbjct: 241 FTSNNKVSNVEESKLNRKGQEDEIVEDLMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300

Query: 301 YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT 360
           YLDGIKLVFESEKADWGR EKVIGCGFDPKQKKVFFTVDSELVHVI+CKSEEFGSPLYPT
Sbjct: 301 YLDGIKLVFESEKADWGRPEKVIGCGFDPKQKKVFFTVDSELVHVINCKSEEFGSPLYPT 360

Query: 361 LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID 420
           LAANGDVTVLVNLGQS FKYIPAQRTPNPCFVSPLVNN  GFHGNGYEDSRELFSM MID
Sbjct: 361 LAANGDVTVLVNLGQSAFKYIPAQRTPNPCFVSPLVNNTGGFHGNGYEDSRELFSMGMID 420

Query: 421 SQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEERIGSKT 473
           SQWFSRLTPKPSNNLVGD+REDDELSNDMESC EIELFEIVVE+EERIGSKT
Sbjct: 421 SQWFSRLTPKPSNNLVGDNREDDELSNDMESCDEIELFEIVVEDEERIGSKT 472

BLAST of CSPI04G24390 vs. ExPASy TrEMBL
Match: A0A1S3BTL1 (uncharacterized protein LOC103493567 OS=Cucumis melo OX=3656 GN=LOC103493567 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.8e-250
Identity = 446/472 (94.49%), Postives = 455/472 (96.40%), Query Frame = 0

Query: 1   MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL 60
           MVGTSVVAVAAV IAVLALLLTGLYFWK+RR GIVESETIGKLQSVESSQQRS SGALKL
Sbjct: 1   MVGTSVVAVAAVSIAVLALLLTGLYFWKQRRGGIVESETIGKLQSVESSQQRSGSGALKL 60

Query: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS 120
           HHQSESEGKRRLSNFYPRGVSQK LFSWDDSPSLVNDAVENGWTQFAFTDY SSSPTSRS
Sbjct: 61  HHQSESEGKRRLSNFYPRGVSQKTLFSWDDSPSLVNDAVENGWTQFAFTDYTSSSPTSRS 120

Query: 121 RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT 180
           RLLGLCSASEIEKEIP+ EISWEVS GSADFMQKIRLNSGFKK+INNTISSYPASSVI+T
Sbjct: 121 RLLGLCSASEIEKEIPDTEISWEVSPGSADFMQKIRLNSGFKKIINNTISSYPASSVIRT 180

Query: 181 ALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASSESLAY 240
           ALPLPGPPLASFPQEAYFEITILNI GDENEPTG AKEGERIKLIPENHSSKASSESLAY
Sbjct: 181 ALPLPGPPLASFPQEAYFEITILNISGDENEPTGAAKEGERIKLIPENHSSKASSESLAY 240

Query: 241 FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300
           FTSNNKVSNVEESKL+ KGQEDEIVE +MLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV
Sbjct: 241 FTSNNKVSNVEESKLNRKGQEDEIVEDLMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300

Query: 301 YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT 360
           YLDGIKLVFESEKADWGR EKVIGCGFDPKQKKVFFTVDSELVHVI+CKSEEFGSPLYPT
Sbjct: 301 YLDGIKLVFESEKADWGRPEKVIGCGFDPKQKKVFFTVDSELVHVINCKSEEFGSPLYPT 360

Query: 361 LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID 420
           LAANGDVTVLVNLGQS FKYIPAQRTPNPCFVSPLVNN  GFHGNGYEDSRELFSM MID
Sbjct: 361 LAANGDVTVLVNLGQSAFKYIPAQRTPNPCFVSPLVNNTGGFHGNGYEDSRELFSMGMID 420

Query: 421 SQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEERIGSKT 473
           SQWFSRLTPKPSNNLVGD+REDDELSNDMESC EIELFEIVVE+EERIGSKT
Sbjct: 421 SQWFSRLTPKPSNNLVGDNREDDELSNDMESCDEIELFEIVVEDEERIGSKT 472

BLAST of CSPI04G24390 vs. ExPASy TrEMBL
Match: A0A5D3D9K3 (SPla/RYanodine receptor SPRY OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003110 PE=4 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.1e-249
Identity = 445/472 (94.28%), Postives = 454/472 (96.19%), Query Frame = 0

Query: 1   MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL 60
           MVGTSVVAVAAV IAVLALLLTGLYFWK+RR GIVESETIGKLQSVESSQQRS SGALKL
Sbjct: 1   MVGTSVVAVAAVSIAVLALLLTGLYFWKQRRGGIVESETIGKLQSVESSQQRSGSGALKL 60

Query: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS 120
           HHQSESEGKRRLSNFYPRGVSQK LFSWDDSPSLVNDAVENGWTQFAFTDY SSSPTSRS
Sbjct: 61  HHQSESEGKRRLSNFYPRGVSQKTLFSWDDSPSLVNDAVENGWTQFAFTDYTSSSPTSRS 120

Query: 121 RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT 180
           RLLGLCSASEIEKEIP+ EISWEVS GSADFMQKIRLNSGFKK+INN ISSYPASSVI+T
Sbjct: 121 RLLGLCSASEIEKEIPDTEISWEVSPGSADFMQKIRLNSGFKKIINNAISSYPASSVIRT 180

Query: 181 ALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASSESLAY 240
           ALPLPGPPLASFPQEAYFEITILNI GDENEPTG AKEGERIKLIPENHSSKASSESLAY
Sbjct: 181 ALPLPGPPLASFPQEAYFEITILNISGDENEPTGAAKEGERIKLIPENHSSKASSESLAY 240

Query: 241 FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300
           FTSNNKVSNVEESKL+ KGQEDEIVE +MLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV
Sbjct: 241 FTSNNKVSNVEESKLNRKGQEDEIVEDLMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300

Query: 301 YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT 360
           YLDGIKLVFESEKADWGR EKVIGCGFDPKQKKVFFTVDSELVHVI+CKSEEFGSPLYPT
Sbjct: 301 YLDGIKLVFESEKADWGRPEKVIGCGFDPKQKKVFFTVDSELVHVINCKSEEFGSPLYPT 360

Query: 361 LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID 420
           LAANGDVTVLVNLGQS FKYIPAQRTPNPCFVSPLVNN  GFHGNGYEDSRELFSM MID
Sbjct: 361 LAANGDVTVLVNLGQSAFKYIPAQRTPNPCFVSPLVNNTGGFHGNGYEDSRELFSMGMID 420

Query: 421 SQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEERIGSKT 473
           SQWFSRLTPKPSNNLVGD+REDDELSNDMESC EIELFEIVVE+EERIGSKT
Sbjct: 421 SQWFSRLTPKPSNNLVGDNREDDELSNDMESCDEIELFEIVVEDEERIGSKT 472

BLAST of CSPI04G24390 vs. ExPASy TrEMBL
Match: A0A6J1IN93 (uncharacterized protein LOC111476777 OS=Cucurbita maxima OX=3661 GN=LOC111476777 PE=4 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 7.0e-217
Identity = 396/472 (83.90%), Postives = 422/472 (89.41%), Query Frame = 0

Query: 1   MVGTSVVAVAAVCIAVLALLLTGLYFW---KRRRWGIVESETIGKLQSVESSQQRSASGA 60
           M G+S VA AAV IAVLALLL GLYFW   +RRR  ++ESET+  LQSVE+SQQ+S S  
Sbjct: 1   MAGSSAVAAAAVSIAVLALLLIGLYFWWRRRRRRRRLIESETVESLQSVENSQQQSGSRT 60

Query: 61  LKLHHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYV-SSSP 120
           + LHHQSESEG+RRLSNFYPRGVS KPLFSWDDSPSLVNDAVENGWTQFAFTDY+ SSSP
Sbjct: 61  VTLHHQSESEGRRRLSNFYPRGVSPKPLFSWDDSPSLVNDAVENGWTQFAFTDYMSSSSP 120

Query: 121 TSRSRLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYP-AS 180
           TSRSRLLGLC+A +IEKEI EAEISWEVSQGSADFMQKIRLNSGF   I NTISS+P AS
Sbjct: 121 TSRSRLLGLCTAGDIEKEIAEAEISWEVSQGSADFMQKIRLNSGFNNTITNTISSFPSAS 180

Query: 181 SVIKTALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASS 240
           SVI+TALPLPGPPLASFPQEAYFEITIL+I GDENEPTG AKEGERIKLIPENH SK SS
Sbjct: 181 SVIRTALPLPGPPLASFPQEAYFEITILSISGDENEPTGAAKEGERIKLIPENHCSKTSS 240

Query: 241 ESLAYFTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFN 300
           ESLAYFTSNNKVSNVEESKL+GKG+EDE VE VMLS+GL  G SAPSKLPGSYSGSIGFN
Sbjct: 241 ESLAYFTSNNKVSNVEESKLNGKGEEDEAVEDVMLSIGLTFGASAPSKLPGSYSGSIGFN 300

Query: 301 SNGSVYLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGS 360
           SNGSVYLDGIKLVFESE+ +WGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGS
Sbjct: 301 SNGSVYLDGIKLVFESEREEWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGS 360

Query: 361 PLYPTLAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFS 420
           PLYPTLAAN DVTVLVNLGQS+FKYI AQRTPNPCFVS LVN A GFHGNGYEDSRELFS
Sbjct: 361 PLYPTLAANADVTVLVNLGQSMFKYITAQRTPNPCFVSQLVNAAGGFHGNGYEDSRELFS 420

Query: 421 MNMIDSQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEER 468
           M MIDSQWFSRLTPK +NN V DHREDDE+S+      EIELFEIVVE+E+R
Sbjct: 421 MGMIDSQWFSRLTPKSTNNFVADHREDDEMSD------EIELFEIVVEDEKR 466

BLAST of CSPI04G24390 vs. NCBI nr
Match: XP_004141270.1 (uncharacterized protein LOC101217358 [Cucumis sativus] >KGN55224.1 hypothetical protein Csa_012033 [Cucumis sativus])

HSP 1 Score: 928.7 bits (2399), Expect = 2.0e-266
Identity = 470/472 (99.58%), Postives = 471/472 (99.79%), Query Frame = 0

Query: 1   MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL 60
           MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL
Sbjct: 1   MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL 60

Query: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS 120
           HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS
Sbjct: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS 120

Query: 121 RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT 180
           RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT
Sbjct: 121 RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT 180

Query: 181 ALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASSESLAY 240
           ALPLPGPPLASFPQEAYFEITILNIYGDEN+PTGTAKEGERIKLIPENHSSKASSESLAY
Sbjct: 181 ALPLPGPPLASFPQEAYFEITILNIYGDENQPTGTAKEGERIKLIPENHSSKASSESLAY 240

Query: 241 FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300
           FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV
Sbjct: 241 FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300

Query: 301 YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT 360
           YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT
Sbjct: 301 YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT 360

Query: 361 LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID 420
           LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID
Sbjct: 361 LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID 420

Query: 421 SQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEERIGSKT 473
           SQWFSRLTPKPSNNLV DHREDDELSNDMESCVEIELFEIVVENEERIGSKT
Sbjct: 421 SQWFSRLTPKPSNNLVDDHREDDELSNDMESCVEIELFEIVVENEERIGSKT 472

BLAST of CSPI04G24390 vs. NCBI nr
Match: XP_008452586.1 (PREDICTED: uncharacterized protein LOC103493567 [Cucumis melo] >KAA0064408.1 SPla/RYanodine receptor SPRY [Cucumis melo var. makuwa])

HSP 1 Score: 874.0 bits (2257), Expect = 5.8e-250
Identity = 446/472 (94.49%), Postives = 455/472 (96.40%), Query Frame = 0

Query: 1   MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL 60
           MVGTSVVAVAAV IAVLALLLTGLYFWK+RR GIVESETIGKLQSVESSQQRS SGALKL
Sbjct: 1   MVGTSVVAVAAVSIAVLALLLTGLYFWKQRRGGIVESETIGKLQSVESSQQRSGSGALKL 60

Query: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS 120
           HHQSESEGKRRLSNFYPRGVSQK LFSWDDSPSLVNDAVENGWTQFAFTDY SSSPTSRS
Sbjct: 61  HHQSESEGKRRLSNFYPRGVSQKTLFSWDDSPSLVNDAVENGWTQFAFTDYTSSSPTSRS 120

Query: 121 RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT 180
           RLLGLCSASEIEKEIP+ EISWEVS GSADFMQKIRLNSGFKK+INNTISSYPASSVI+T
Sbjct: 121 RLLGLCSASEIEKEIPDTEISWEVSPGSADFMQKIRLNSGFKKIINNTISSYPASSVIRT 180

Query: 181 ALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASSESLAY 240
           ALPLPGPPLASFPQEAYFEITILNI GDENEPTG AKEGERIKLIPENHSSKASSESLAY
Sbjct: 181 ALPLPGPPLASFPQEAYFEITILNISGDENEPTGAAKEGERIKLIPENHSSKASSESLAY 240

Query: 241 FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300
           FTSNNKVSNVEESKL+ KGQEDEIVE +MLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV
Sbjct: 241 FTSNNKVSNVEESKLNRKGQEDEIVEDLMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300

Query: 301 YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT 360
           YLDGIKLVFESEKADWGR EKVIGCGFDPKQKKVFFTVDSELVHVI+CKSEEFGSPLYPT
Sbjct: 301 YLDGIKLVFESEKADWGRPEKVIGCGFDPKQKKVFFTVDSELVHVINCKSEEFGSPLYPT 360

Query: 361 LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID 420
           LAANGDVTVLVNLGQS FKYIPAQRTPNPCFVSPLVNN  GFHGNGYEDSRELFSM MID
Sbjct: 361 LAANGDVTVLVNLGQSAFKYIPAQRTPNPCFVSPLVNNTGGFHGNGYEDSRELFSMGMID 420

Query: 421 SQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEERIGSKT 473
           SQWFSRLTPKPSNNLVGD+REDDELSNDMESC EIELFEIVVE+EERIGSKT
Sbjct: 421 SQWFSRLTPKPSNNLVGDNREDDELSNDMESCDEIELFEIVVEDEERIGSKT 472

BLAST of CSPI04G24390 vs. NCBI nr
Match: TYK20179.1 (SPla/RYanodine receptor SPRY [Cucumis melo var. makuwa])

HSP 1 Score: 872.1 bits (2252), Expect = 2.2e-249
Identity = 445/472 (94.28%), Postives = 454/472 (96.19%), Query Frame = 0

Query: 1   MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL 60
           MVGTSVVAVAAV IAVLALLLTGLYFWK+RR GIVESETIGKLQSVESSQQRS SGALKL
Sbjct: 1   MVGTSVVAVAAVSIAVLALLLTGLYFWKQRRGGIVESETIGKLQSVESSQQRSGSGALKL 60

Query: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS 120
           HHQSESEGKRRLSNFYPRGVSQK LFSWDDSPSLVNDAVENGWTQFAFTDY SSSPTSRS
Sbjct: 61  HHQSESEGKRRLSNFYPRGVSQKTLFSWDDSPSLVNDAVENGWTQFAFTDYTSSSPTSRS 120

Query: 121 RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPASSVIKT 180
           RLLGLCSASEIEKEIP+ EISWEVS GSADFMQKIRLNSGFKK+INN ISSYPASSVI+T
Sbjct: 121 RLLGLCSASEIEKEIPDTEISWEVSPGSADFMQKIRLNSGFKKIINNAISSYPASSVIRT 180

Query: 181 ALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASSESLAY 240
           ALPLPGPPLASFPQEAYFEITILNI GDENEPTG AKEGERIKLIPENHSSKASSESLAY
Sbjct: 181 ALPLPGPPLASFPQEAYFEITILNISGDENEPTGAAKEGERIKLIPENHSSKASSESLAY 240

Query: 241 FTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300
           FTSNNKVSNVEESKL+ KGQEDEIVE +MLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV
Sbjct: 241 FTSNNKVSNVEESKLNRKGQEDEIVEDLMLSVGLISGGSAPSKLPGSYSGSIGFNSNGSV 300

Query: 301 YLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPT 360
           YLDGIKLVFESEKADWGR EKVIGCGFDPKQKKVFFTVDSELVHVI+CKSEEFGSPLYPT
Sbjct: 301 YLDGIKLVFESEKADWGRPEKVIGCGFDPKQKKVFFTVDSELVHVINCKSEEFGSPLYPT 360

Query: 361 LAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMID 420
           LAANGDVTVLVNLGQS FKYIPAQRTPNPCFVSPLVNN  GFHGNGYEDSRELFSM MID
Sbjct: 361 LAANGDVTVLVNLGQSAFKYIPAQRTPNPCFVSPLVNNTGGFHGNGYEDSRELFSMGMID 420

Query: 421 SQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEERIGSKT 473
           SQWFSRLTPKPSNNLVGD+REDDELSNDMESC EIELFEIVVE+EERIGSKT
Sbjct: 421 SQWFSRLTPKPSNNLVGDNREDDELSNDMESCDEIELFEIVVEDEERIGSKT 472

BLAST of CSPI04G24390 vs. NCBI nr
Match: XP_038897299.1 (uncharacterized protein LOC120085411 [Benincasa hispida])

HSP 1 Score: 841.6 bits (2173), Expect = 3.2e-240
Identity = 426/473 (90.06%), Postives = 446/473 (94.29%), Query Frame = 0

Query: 1   MVGTSVVAVAAVCIAVLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGALKL 60
           M G+SVVAVAAV IAVLALLLTGLYFW+RRR G+VESETI KLQSVE+SQQRS SG LKL
Sbjct: 1   MAGSSVVAVAAVSIAVLALLLTGLYFWRRRRRGLVESETIEKLQSVENSQQRSGSGTLKL 60

Query: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVSSSPTSRS 120
           HHQSESEGKRRLSNFYPRGVSQKPLFSWDD+PSLVNDAVENGWTQFAFTDY SSSPTSRS
Sbjct: 61  HHQSESEGKRRLSNFYPRGVSQKPLFSWDDNPSLVNDAVENGWTQFAFTDYTSSSPTSRS 120

Query: 121 RLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYP-ASSVIK 180
           R+LGLC+A EIEKEIPEAEISWEVSQGSADFMQ+IRLNSGFK  INNTISSYP ASS I+
Sbjct: 121 RMLGLCTAGEIEKEIPEAEISWEVSQGSADFMQRIRLNSGFKNTINNTISSYPSASSAIR 180

Query: 181 TALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASSESLA 240
           T LPLPGPPLASFPQEAYFEITILNI G ENEPTG +KEGERIKLIPENHSSKASSESLA
Sbjct: 181 TTLPLPGPPLASFPQEAYFEITILNISGHENEPTGASKEGERIKLIPENHSSKASSESLA 240

Query: 241 YFTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSNGS 300
           YFTSNNKVSNVEESK++GKG++DE+VE VM+SVGLISGGSAPSKLPGSYSGSIGFNSNGS
Sbjct: 241 YFTSNNKVSNVEESKINGKGEKDEVVEDVMVSVGLISGGSAPSKLPGSYSGSIGFNSNGS 300

Query: 301 VYLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYP 360
           VYLDGIKLVFESE+ADWGR EKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYP
Sbjct: 301 VYLDGIKLVFESERADWGRPEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYP 360

Query: 361 TLAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMI 420
           TLAAN DVTV VNLGQSVFKYIPAQRTPNPCFVSPLVN A GFHGNGYEDSRELFSM MI
Sbjct: 361 TLAANADVTVSVNLGQSVFKYIPAQRTPNPCFVSPLVNTAGGFHGNGYEDSRELFSMGMI 420

Query: 421 DSQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEERIGSKT 473
           DSQWFSRLTPKPSNN+V DHREDDELSNDME C EIELFEIVVE+E+RI SKT
Sbjct: 421 DSQWFSRLTPKPSNNVVVDHREDDELSNDMEICDEIELFEIVVEDEQRIASKT 473

BLAST of CSPI04G24390 vs. NCBI nr
Match: KAG7024266.1 (hypothetical protein SDJN02_13080, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 767.7 bits (1981), Expect = 5.9e-218
Identity = 399/470 (84.89%), Postives = 425/470 (90.43%), Query Frame = 0

Query: 1   MVGTSVVAVAAVCIAVLALLLTGLYF-WKRRRWGIVESETIGKLQSVESSQQRSASGALK 60
           MVG+S VA AAV IAVLALLL GLYF W+RRR  ++ESET+  LQSVE+SQQ+S S  + 
Sbjct: 1   MVGSSAVAAAAVSIAVLALLLIGLYFWWRRRRRRLIESETVETLQSVENSQQQSGSRTVT 60

Query: 61  LHHQSESEGKRRLSNFYPRGVSQKPLFSWDDSPSLVNDAVENGWTQFAFTDYVS-SSPTS 120
           LHHQSESEG+RRLSNFYPRGVS KPLFSWDDSPSLVNDAVENGWTQFAFTDY+S SSPTS
Sbjct: 61  LHHQSESEGRRRLSNFYPRGVSPKPLFSWDDSPSLVNDAVENGWTQFAFTDYMSLSSPTS 120

Query: 121 RSRLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYP-ASSV 180
           RSRLLGLC+A EIEKEI EAEISWEVSQGSADFMQKIRLNSGF   I NTISS+P ASSV
Sbjct: 121 RSRLLGLCTAGEIEKEIAEAEISWEVSQGSADFMQKIRLNSGFNNTITNTISSFPSASSV 180

Query: 181 IKTALPLPGPPLASFPQEAYFEITILNIYGDENEPTGTAKEGERIKLIPENHSSKASSES 240
           I+TALPLPGPPLASFPQEAYFEITIL+I GDENEPTG AKEGERIKLIPENH SK SSES
Sbjct: 181 IRTALPLPGPPLASFPQEAYFEITILSISGDENEPTGAAKEGERIKLIPENHCSKTSSES 240

Query: 241 LAYFTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGSAPSKLPGSYSGSIGFNSN 300
           LAYFTSNNKVSNVEESKL+GKG+EDE VE VMLS+GL SG SAPSKLPGSYSGSIGFNSN
Sbjct: 241 LAYFTSNNKVSNVEESKLNGKGEEDEAVEDVMLSIGLTSGTSAPSKLPGSYSGSIGFNSN 300

Query: 301 GSVYLDGIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPL 360
           GSVYLDGIKLVFESE+ +WGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPL
Sbjct: 301 GSVYLDGIKLVFESEREEWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPL 360

Query: 361 YPTLAANGDVTVLVNLGQSVFKYIPAQRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMN 420
           YPTLAAN DVT+LVNLGQS+FKYI AQRTPNPCFVS LVN A GFHGNGYEDSRELFSM 
Sbjct: 361 YPTLAANADVTILVNLGQSMFKYITAQRTPNPCFVSQLVNAAGGFHGNGYEDSRELFSMG 420

Query: 421 MIDSQWFSRLTPKPSNNLVGDHREDDELSNDMESCVEIELFEIVVENEER 468
           MIDSQWFSRLTPK +NNLV DHREDDE+S+      EIELFEIVVE+E+R
Sbjct: 421 MIDSQWFSRLTPKSTNNLVVDHREDDEMSD------EIELFEIVVEDEKR 464

BLAST of CSPI04G24390 vs. TAIR 10
Match: AT4G06536.1 (SPla/RYanodine receptor (SPRY) domain-containing protein )

HSP 1 Score: 149.4 bits (376), Expect = 7.1e-36
Identity = 85/176 (48.30%), Postives = 112/176 (63.64%), Query Frame = 0

Query: 304 GIKLVFESEKADWGRAEKVIGCGFDPKQKKVFFTVDSELVHVIHCKSEEFGSPLYPTLAA 363
           G+KLV ESEK +W +  KV+GCG+DP++KKV+FTV+S LVHVI+CK++EFG+PLYPTLA+
Sbjct: 3   GMKLVCESEKHEWAKENKVVGCGYDPRKKKVYFTVNSHLVHVINCKADEFGTPLYPTLAS 62

Query: 364 NGDVTVLVNLGQSVFKYIPA--QRTPNPCFVSPLVNNADGFHGNGYEDSRELFSMNMIDS 423
           N +  VLVNLGQS F Y PA  QRT NPC  +            G EDS+ELFS+  IDS
Sbjct: 63  NTEARVLVNLGQSPFYYSPANGQRTSNPCAGAVA----------GLEDSKELFSIGRIDS 122

Query: 424 QWFSRLTPKPSNN--------LVGD-HREDDELSNDMESCVEIELFEIVVENEERI 469
            + S       N+         VG+ HR   +L  D ES  + +LFEI +E   ++
Sbjct: 123 HYLSNFFINRGNSDEIASGRATVGNSHRR--KLDYDEES--DADLFEIALERSGKL 164

BLAST of CSPI04G24390 vs. TAIR 10
Match: AT4G06534.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; Has 22 Blast hits to 22 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 22; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 141.7 bits (356), Expect = 1.5e-33
Identity = 114/321 (35.51%), Postives = 159/321 (49.53%), Query Frame = 0

Query: 16  VLALLLTGLYFWKRRRWGIVESETIGKLQSVESSQQRSASGAL-KLH-----HQSESEGK 75
           V  ++L+ L     RRW  +  E    L++    +  S    + KLH     HQ ++   
Sbjct: 12  VSVVVLSSLLVVFLRRWCCLRREITTTLKAASPIRSDSFQARISKLHQTSLIHQLDTSDI 71

Query: 76  RRLSNFYPRGVSQ---------KP-LFSWDDSPSLVNDAVENGWTQFAFTDY----VSSS 135
           +R  N     +S+         KP LF W D P+LV +AVENGWT+F F  +    + S 
Sbjct: 72  KRRGNIKNYSISRTATAGGFPSKPGLFIWTDHPALVTEAVENGWTRFGFAVHEPAPLVSG 131

Query: 136 PTSRSRLLGLCSASEIEKEIPEAEISWEVSQGSADFMQKIRLNSGFKKMINNTISSYPAS 195
            +  S LLGLC+ +    E P   ISWEVS GS +F Q I+ N  F    N T+++    
Sbjct: 132 ASPGSVLLGLCTTA--GSEDPGVVISWEVSNGSVEFTQTIKFNQAF----NETVNAKKHL 191

Query: 196 SVIKTALPLPGPPLAS--FPQEAYFEITILNI----YGDENEPTGTAKEGERIKLIPENH 255
            +++  LPLPGP L S  FPQEAYFEITIL I     G+  +      EGE+ KL     
Sbjct: 192 MILRAGLPLPGPQLISSAFPQEAYFEITILEITQHHSGEAGDVGCDLVEGEKTKLF---- 251

Query: 256 SSKASSESLAYFTSNNKVSNVEESKLDGKGQEDEIVEGVMLSVGLISGGS---APSKLPG 308
                         N  +  V+ S+ DGK +E       +LS+GL +GGS     ++LPG
Sbjct: 252 -------------KNQGLKLVKRSEWDGKNEE------AVLSLGLATGGSFGAGETRLPG 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q96UB66.3e-0531.40Protein ssh4 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 /... [more]
Q4WRW01.8e-0430.58Protein ssh4 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 ... [more]
A1CNW83.1e-0432.23Protein ssh4 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / ... [more]
O744974.1e-0430.17SPRY domain-containing protein C285.10c OS=Schizosaccharomyces pombe (strain 972... [more]
Match NameE-valueIdentityDescription
A0A0A0L2G09.6e-26799.58B30.2/SPRY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G641570 ... [more]
A0A5A7V9Y82.8e-25094.49SPla/RYanodine receptor SPRY OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3BTL12.8e-25094.49uncharacterized protein LOC103493567 OS=Cucumis melo OX=3656 GN=LOC103493567 PE=... [more]
A0A5D3D9K31.1e-24994.28SPla/RYanodine receptor SPRY OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1IN937.0e-21783.90uncharacterized protein LOC111476777 OS=Cucurbita maxima OX=3661 GN=LOC111476777... [more]
Match NameE-valueIdentityDescription
XP_004141270.12.0e-26699.58uncharacterized protein LOC101217358 [Cucumis sativus] >KGN55224.1 hypothetical ... [more]
XP_008452586.15.8e-25094.49PREDICTED: uncharacterized protein LOC103493567 [Cucumis melo] >KAA0064408.1 SPl... [more]
TYK20179.12.2e-24994.28SPla/RYanodine receptor SPRY [Cucumis melo var. makuwa][more]
XP_038897299.13.2e-24090.06uncharacterized protein LOC120085411 [Benincasa hispida][more]
KAG7024266.15.9e-21884.89hypothetical protein SDJN02_13080, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT4G06536.17.1e-3648.30SPla/RYanodine receptor (SPRY) domain-containing protein [more]
AT4G06534.11.5e-3335.51unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043136B30.2/SPRY domain superfamilyGENE3D2.60.120.920coord: 177..386
e-value: 2.0E-16
score: 62.4
NoneNo IPR availablePANTHERPTHR12864RAN BINDING PROTEIN 9-RELATEDcoord: 5..465
NoneNo IPR availablePANTHERPTHR12864:SF57SPLA/RYANODINE RECEPTOR (SPRY) DOMAIN PROTEINcoord: 5..465
IPR001870B30.2/SPRY domainPROSITEPS50188B302_SPRYcoord: 185..378
score: 9.164915
IPR044736Ran-binding protein Vid30/RanBPM/SPLA, SPRY domainCDDcd12885SPRY_RanBP_likecoord: 266..374
e-value: 2.09855E-14
score: 68.0748
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 282..380

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G24390.1CSPI04G24390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane