CSPI04G24220 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G24220
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionheat stress transcription factor B-4
LocationChr4: 22062319 .. 22063823 (-)
RNA-Seq ExpressionCSPI04G24220
SyntenyCSPI04G24220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCTTTTCTTATATATTTATTTGAAAAACAAAAAAAAAACATTGTCACGGGTTTGTCTTGAAACCCTCTTATTTCACAGACACATATTCTCTCACTCTCCCACAAAAACCCACCTTCCCAAAATCTCTTAATATAAAATCAAAAAACACACACTAAACTACACAATACCTTTCCTCTTTCCCCTTCCCCCCCTCCCGGCCCCTTCATCTGATCTTCTCAAATGGCTCTAATGCTTGATACTTGTGATGGCGTTTTACTTTCTTTAGACTCACACAAAGCAATCCCAGCTCCCTTTTTAACCAAAACTTACCAACTTGTCGATGATCCTTCCACTGACCATATTGTTTCATGGGGTGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTCCTCCCTAACTATTTCAAACACAACAATTTCTCTAGCTTTGTACGCCAGCTCAACACTTATGTATGTTCTCTTCATCATCATCATCATCATCATCATTTTTTTTCTATACAACTTTCAATCCCCATTTATTTATTTAACTTGAATTTCTCTTTTTCTATTTAGGGTTTTAGAAAAATTGTACCTGATAGATGGGAGTTTGCGAACGAGTTCTTTAGAAAAGGGGAGAAGCATTTATTATGTGAGATCCATAGACGGAAAACAGCTCAACCACAAGTTACCGTTAACCAACACCATCAACCTCATTCTCCACTCAATCCCGGTTTCTACCATTTCCCCACTGCTCGACTTAGTATTTCCCCTTCCGATTCCGACGACCAAAACAATTACTGGTGTGACTCGCCATCGCCCAACAATAATAATAACAACTCCGTTACCGCTCTGTCGGAAGACAATGAACGACTCCGTCGGAGTAACAATATGCTGATGTCGGAGTTAGCCCATATGAAAAAACTCTACAACGATATCATTTATTTCGTTCAAAACCACGTGAAGCCGGTTGCTCCCAGTAATTCATATCAATACTCAACCACCACGTCGTTACTCTCCGATGGGTTTCCGGTTGTACGACAACCCAACCATTACCATCATCATCATCATCATCATCAACAAGTATCGAGTCAGATCCGGAATAATACCGTTGGAACGAAAAGCTTCGTGACGATTCTAGAAGAAGAACAACAACAACAAACAAAAACGAAGCTTTTCGGTGTGGCGATTCAATCGAAGAAACGGTTACATCCAGAGTATGGTAATTCTAATAGCAACAACAACAACAACAAAGCTAGATTGGTTTTGGAAAAAGACGATTTAGGCCTTAATCTCATGCCTCCTTCTGCTTGTTAATTAGTTCTGATATATTTTTTTCTTTTTTGGAGGGAATTTTTGTTAGAAATCCCAGAGGGGGAGTCGGATCCTTTTTTCATTGCTGCATTATTTCATATGATCATAATTGAAATTAGAAAAATGTGTGCGTGCGTTTAATTTGTGATAGCACTGTAAGTTATCTTCTATATGTATATAACCCTCATTGACGC

mRNA sequence

CTTTCTTTTCTTATATATTTATTTGAAAAACAAAAAAAAAACATTGTCACGGGTTTGTCTTGAAACCCTCTTATTTCACAGACACATATTCTCTCACTCTCCCACAAAAACCCACCTTCCCAAAATCTCTTAATATAAAATCAAAAAACACACACTAAACTACACAATACCTTTCCTCTTTCCCCTTCCCCCCCTCCCGGCCCCTTCATCTGATCTTCTCAAATGGCTCTAATGCTTGATACTTGTGATGGCGTTTTACTTTCTTTAGACTCACACAAAGCAATCCCAGCTCCCTTTTTAACCAAAACTTACCAACTTGTCGATGATCCTTCCACTGACCATATTGTTTCATGGGGTGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTCCTCCCTAACTATTTCAAACACAACAATTTCTCTAGCTTTGTACGCCAGCTCAACACTTATGGTTTTAGAAAAATTGTACCTGATAGATGGGAGTTTGCGAACGAGTTCTTTAGAAAAGGGGAGAAGCATTTATTATGTGAGATCCATAGACGGAAAACAGCTCAACCACAAGTTACCGTTAACCAACACCATCAACCTCATTCTCCACTCAATCCCGGTTTCTACCATTTCCCCACTGCTCGACTTAGTATTTCCCCTTCCGATTCCGACGACCAAAACAATTACTGGTGTGACTCGCCATCGCCCAACAATAATAATAACAACTCCGTTACCGCTCTGTCGGAAGACAATGAACGACTCCGTCGGAGTAACAATATGCTGATGTCGGAGTTAGCCCATATGAAAAAACTCTACAACGATATCATTTATTTCGTTCAAAACCACGTGAAGCCGGTTGCTCCCAGTAATTCATATCAATACTCAACCACCACGTCGTTACTCTCCGATGGGTTTCCGGTTGTACGACAACCCAACCATTACCATCATCATCATCATCATCATCAACAAGTATCGAGTCAGATCCGGAATAATACCGTTGGAACGAAAAGCTTCGTGACGATTCTAGAAGAAGAACAACAACAACAAACAAAAACGAAGCTTTTCGGTGTGGCGATTCAATCGAAGAAACGGTTACATCCAGAGTATGGTAATTCTAATAGCAACAACAACAACAACAAAGCTAGATTGGTTTTGGAAAAAGACGATTTAGGCCTTAATCTCATGCCTCCTTCTGCTTGTTAATTAGTTCTGATATATTTTTTTCTTTTTTGGAGGGAATTTTTGTTAGAAATCCCAGAGGGGGAGTCGGATCCTTTTTTCATTGCTGCATTATTTCATATGATCATAATTGAAATTAGAAAAATGTGTGCGTGCGTTTAATTTGTGATAGCACTGTAAGTTATCTTCTATATGTATATAACCCTCATTGACGC

Coding sequence (CDS)

ATGGCTCTAATGCTTGATACTTGTGATGGCGTTTTACTTTCTTTAGACTCACACAAAGCAATCCCAGCTCCCTTTTTAACCAAAACTTACCAACTTGTCGATGATCCTTCCACTGACCATATTGTTTCATGGGGTGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTCCTCCCTAACTATTTCAAACACAACAATTTCTCTAGCTTTGTACGCCAGCTCAACACTTATGGTTTTAGAAAAATTGTACCTGATAGATGGGAGTTTGCGAACGAGTTCTTTAGAAAAGGGGAGAAGCATTTATTATGTGAGATCCATAGACGGAAAACAGCTCAACCACAAGTTACCGTTAACCAACACCATCAACCTCATTCTCCACTCAATCCCGGTTTCTACCATTTCCCCACTGCTCGACTTAGTATTTCCCCTTCCGATTCCGACGACCAAAACAATTACTGGTGTGACTCGCCATCGCCCAACAATAATAATAACAACTCCGTTACCGCTCTGTCGGAAGACAATGAACGACTCCGTCGGAGTAACAATATGCTGATGTCGGAGTTAGCCCATATGAAAAAACTCTACAACGATATCATTTATTTCGTTCAAAACCACGTGAAGCCGGTTGCTCCCAGTAATTCATATCAATACTCAACCACCACGTCGTTACTCTCCGATGGGTTTCCGGTTGTACGACAACCCAACCATTACCATCATCATCATCATCATCATCAACAAGTATCGAGTCAGATCCGGAATAATACCGTTGGAACGAAAAGCTTCGTGACGATTCTAGAAGAAGAACAACAACAACAAACAAAAACGAAGCTTTTCGGTGTGGCGATTCAATCGAAGAAACGGTTACATCCAGAGTATGGTAATTCTAATAGCAACAACAACAACAACAAAGCTAGATTGGTTTTGGAAAAAGACGATTTAGGCCTTAATCTCATGCCTCCTTCTGCTTGTTAA

Protein sequence

MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQPNHYHHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQTKTKLFGVAIQSKKRLHPEYGNSNSNNNNNKARLVLEKDDLGLNLMPPSAC*
Homology
[Close]
BLAST of CSPI04G24220 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 6.0e-87
Identity = 185/331 (55.89%), Postives = 228/331 (68.88%), Query Frame = 0 

Query: 19  KAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 78
           KA+PAPFLTKTYQLVDDP+TDH+VSWG+DDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 79  QLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ--PQ----VTVNQHHQPHSP 138
           QLNTYGFRKIVPDRWEFANEFF++GEKHLLCEIHRRKT+Q  PQ       + H  P  P
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMSHHHAPPQIP 148

Query: 139 LNPG-FYHFPTARLSISPSDSDDQNNYWCDSPSPNN--------NNNNSVTALSEDNERL 198
            + G F+  P  R++     + ++++YWCD   P+         +    VTALSEDNERL
Sbjct: 149 FSGGSFFPLPPPRVT-----TPEEDHYWCDDSPPSRPRVIPQQIDTAAQVTALSEDNERL 208

Query: 199 RRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQPNHY 258
           RRSN +LMSELAHMKKLYNDIIYFVQNHVKPVAPSN+  Y   +S L            Y
Sbjct: 209 RRSNTVLMSELAHMKKLYNDIIYFVQNHVKPVAPSNNSSY--LSSFLQKQQQQQPPTLDY 268

Query: 259 HHHHHHHQQVSSQIRNNTVGTKSFVTILEE------EQQQQTKTKLFGVAI-QSKKRLHP 318
           ++    +    + + ++   ++S +T+LE+      +Q    KTKLFGV++  SKKR H 
Sbjct: 269 YNTATVNATNLNALNSSPPTSQSSITVLEDDHTNHHDQSNMRKTKLFGVSLPSSKKRSH- 328

Query: 319 EYGNSNSNNNNNKARLVLEKDDLGLNLMPPS 328
                + ++  +K RLVL++ DL LNLM  S
Sbjct: 329 -----HFSDQTSKTRLVLDQSDLALNLMTAS 346

[Close]
BLAST of CSPI04G24220 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.9e-69
Identity = 140/255 (54.90%), Postives = 165/255 (64.71%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLD----------SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA +++ C  +++S++          + K +PAPFLTKTYQLVDDP TDHIVSWGEDDTT
Sbjct: 1   MAFLVERCGEMVVSMEMGPHGGGGAAAGKPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLAE 120

Query: 121 IHRRKTAQPQVTVNQHHQPHSPLNPGFYHFPTARLSISPS----------DSDDQNNYWC 180
           IHRRK++QP         P  P  P  +H      S+ P             +      C
Sbjct: 121 IHRRKSSQPP-------PPPMPHQPYHHHHHLNPFSLPPPPPAYHHHHLIQEEPATTAHC 180

Query: 181 DSPSPNNNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNS 236
                     + + ALSEDN +LRR N++L+SELAHMKKLYNDIIYF+QNHV PV  + +
Sbjct: 181 TVAGDGGEGGDFLAALSEDNRQLRRRNSLLLSELAHMKKLYNDIIYFLQNHVAPVTTTTT 240

[Close]
BLAST of CSPI04G24220 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.7e-65
Identity = 140/237 (59.07%), Postives = 169/237 (71.31%), Query Frame = 0 

Query: 1   MALMLDTCDG-VLLSLD-SH----------KAIPAPFLTKTYQLVDDPSTDHIVSWGEDD 60
           MA +++ C G +++S++ SH           A PAPFL+KTYQLVDDPSTD +VSWGED+
Sbjct: 1   MAFLVERCGGEMVVSMERSHGRSTTTAAAVTAAPAPFLSKTYQLVDDPSTDDVVSWGEDE 60

Query: 61  TTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLL 120
            TFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL
Sbjct: 61  ATFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLL 120

Query: 121 CEIHRRKT---AQPQVTVNQHHQPHSPLNPGFYHFPTARLS----ISPSDSDDQNNYWCD 180
            EIHRRK+   +QPQ         H PL+   +  PT   S     + + +      +C 
Sbjct: 121 SEIHRRKSSSCSQPQPPPPFPMHQHYPLS--LFSPPTTPRSPPVGAAAAAAYHFQEEYCS 180

Query: 181 SPSP-NNNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAP 218
           SP+       + + ALSEDN +LRR N++L+SELAHM+KLYNDIIYF+QNHV+PVAP
Sbjct: 181 SPADYAGGGGDLLAALSEDNRQLRRRNSLLLSELAHMRKLYNDIIYFLQNHVEPVAP 235

[Close]
BLAST of CSPI04G24220 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 7.1e-64
Identity = 165/384 (42.97%), Postives = 201/384 (52.34%), Query Frame = 0 

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDD-TTFVVWRPPEFARDLLPNYFKHNNFSS 76
           + KA+PAPFLTKTYQLVDDP+TDHIVSWG+D  +TFVVWRPPEFARD+LPNYFKHNNFSS
Sbjct: 17  AQKAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILPNYFKHNNFSS 76

Query: 77  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQPHSPLNP 136
           FVRQLNTYGFRK+VP+RWEFANEFFRKGEK LL EIHRRKT+             SP  P
Sbjct: 77  FVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTSSASTA--------SPSPP 136

Query: 137 GFYHFPTARLSISPSDSDDQNN----------------------YWCD------------ 196
            F+  P   L   P  +  Q++                      +W +            
Sbjct: 137 PFFAPPHFPLFHHPGVAAAQHHHAFVGDDGVVAAHGIGMPFPQPHWREPNLPVATRLLAL 196

Query: 197 ---SPSPNN------NNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHV 256
              +PSP++          +   L E+NERLRRSN  L+ ELAHM+KLYNDIIYFVQNHV
Sbjct: 197 GGPAPSPSSAEAGGAGRAATAAVLMEENERLRRSNTALLQELAHMRKLYNDIIYFVQNHV 256

Query: 257 KPVAPS---------------------NSYQYSTTTSLLSDGFPVVRQPNHYHHHHHHHQ 316
           +PVAPS                     N    S  ++  S    +  +P+      H   
Sbjct: 257 RPVAPSPAAAAFLQGLGMQARKKPAAANVLNNSGGSTTSSSSLTIAEEPSPPPQQQHLAG 316

Query: 317 QVSSQIRNNTVGTKSFVTILEEEQQQQTKTKLFGVAIQ-------SKKRLHPEYGNSNSN 329
           + S     N+   +S              TKLFGV +        SK+   PE       
Sbjct: 317 EKSGGEAGNSSAARS-----------SAPTKLFGVHLSAAPCGAGSKRASSPE--EHPPT 376

[Close]
BLAST of CSPI04G24220 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 5.8e-58
Identity = 125/234 (53.42%), Postives = 150/234 (64.10%), Query Frame = 0 

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDD-----TTFVVWRPPEFARDLLPNYFKHN 76
           S   +PAPFLTKTYQLVDDP+TDH+VSW +DD     ++FVVWRPPEFARD+LPNYFKH+
Sbjct: 18  SASVVPAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHS 77

Query: 77  NFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA----------QPQV 136
           NFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LLCEIHRRK+A           P  
Sbjct: 78  NFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSAAATWPPFPPPPPPF 137

Query: 137 TVNQHHQPHSPLNPG-----------------FYHFPTARLSISPSDSDDQNNYWCDSPS 196
              +H    +    G                  + F +A L ++PS           +P+
Sbjct: 138 FAPRHFAAGAFFRHGDGMLHGRLGALVTTTERRHWFESAALPVAPSSRLLSQLGPVIAPA 197

Query: 197 PNNNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPS 219
                     AL ++N RL R N  L+ ELAHM+KLY+DIIYFVQNHV+PVAPS
Sbjct: 198 RRAAATPEEEALMQENHRLLRGNAALVQELAHMRKLYSDIIYFVQNHVRPVAPS 251

[Close]
BLAST of CSPI04G24220 vs. ExPASy TrEMBL
Match: A0A0A0L4W8 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 6.0e-191
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNSVTALSEDN 180
           VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNSVTALSEDN
Sbjct: 121 VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNSVTALSEDN 180

Query: 181 ERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQP 240
           ERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQP
Sbjct: 181 ERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQP 240

Query: 241 NHYHHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQTKTKLFGVAIQSKKRLHPEYGN 300
           NHYHHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQTKTKLFGVAIQSKKRLHPEYGN
Sbjct: 241 NHYHHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQTKTKLFGVAIQSKKRLHPEYGN 300

Query: 301 SNSNNNNNKARLVLEKDDLGLNLMPPSAC 330
           SNSNNNNNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 SNSNNNNNKARLVLEKDDLGLNLMPPSAC 329

[Close]
BLAST of CSPI04G24220 vs. ExPASy TrEMBL
Match: A0A5A7VAY1 (Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003260 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 7.1e-176
Identity = 318/332 (95.78%), Postives = 321/332 (96.69%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD+CDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDSCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSP-NNNNNNSVTALSED 180
           VTVNQHHQ  SPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSP NNNNNNSVTALSED
Sbjct: 121 VTVNQHHQSLSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180

Query: 181 NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ 240
           NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST+TSLLSDGFPVVRQ
Sbjct: 181 NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSTSLLSDGFPVVRQ 240

Query: 241 PNHYHHHHHHHQQVSSQIRNN-TVGTKSFVTILEEE-QQQQTKTKLFGVAIQSKKRLHPE 300
           PNHYHHHH    QVSSQIRNN TVGTKSFVTILEEE QQQQ KTKLFGVAIQSKKRLHPE
Sbjct: 241 PNHYHHHH----QVSSQIRNNTTVGTKSFVTILEEEQQQQQPKTKLFGVAIQSKKRLHPE 300

Query: 301 YGNSNSNNNNNKARLVLEKDDLGLNLMPPSAC 330
           YGNS+S NNNNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 YGNSHS-NNNNKARLVLEKDDLGLNLMPPSAC 327

[Close]
BLAST of CSPI04G24220 vs. ExPASy TrEMBL
Match: A0A1S3BUY5 (heat stress transcription factor B-4 OS=Cucumis melo OX=3656 GN=LOC103493553 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 7.1e-176
Identity = 318/332 (95.78%), Postives = 321/332 (96.69%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD+CDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDSCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSP-NNNNNNSVTALSED 180
           VTVNQHHQ  SPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSP NNNNNNSVTALSED
Sbjct: 121 VTVNQHHQSLSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180

Query: 181 NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ 240
           NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST+TSLLSDGFPVVRQ
Sbjct: 181 NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSTSLLSDGFPVVRQ 240

Query: 241 PNHYHHHHHHHQQVSSQIRNN-TVGTKSFVTILEEE-QQQQTKTKLFGVAIQSKKRLHPE 300
           PNHYHHHH    QVSSQIRNN TVGTKSFVTILEEE QQQQ KTKLFGVAIQSKKRLHPE
Sbjct: 241 PNHYHHHH----QVSSQIRNNTTVGTKSFVTILEEEQQQQQPKTKLFGVAIQSKKRLHPE 300

Query: 301 YGNSNSNNNNNKARLVLEKDDLGLNLMPPSAC 330
           YGNS+S NNNNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 YGNSHS-NNNNKARLVLEKDDLGLNLMPPSAC 327

[Close]
BLAST of CSPI04G24220 vs. ExPASy TrEMBL
Match: A0A6J1FJH2 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111445920 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 8.5e-145
Identity = 282/346 (81.50%), Postives = 298/346 (86.13%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQPHSP--LNPGFYHFPTARLSISPSDSDDQNNYWCDSP--SPNNNN---NNSV 180
           VTVNQHHQ +SP  LN GFYH+P AR+SISPSDSDD  N WCDSP  S NNNN   NNSV
Sbjct: 121 VTVNQHHQSYSPLGLNAGFYHYP-ARVSISPSDSDDNPN-WCDSPPLSSNNNNNNFNNSV 180

Query: 181 TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDG 240
           TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS  TSLLSDG
Sbjct: 181 TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS--TSLLSDG 240

Query: 241 FPV-VRQPNHY--HHHHHHHQQVSSQI-------RNNTVGTKSFVTILEEEQQQQTKTKL 300
           FPV  RQPNH+   +H  + +QVS Q         NN   +KSFVTI+EE      KTKL
Sbjct: 241 FPVGQRQPNHHLLGYHPTNAKQVSGQTHFVAGTPNNNNGSSKSFVTIVEE-----PKTKL 300

Query: 301 FGVAIQSKKRLHPEYGNSNSNNNNNKARLVLEKDDLGLNLMPPSAC 330
           FGV++QSKKR+HPEYG++N    NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 FGVSLQSKKRVHPEYGSNNIVKENNKARLVLEKDDLGLNLMPPSAC 337

[Close]
BLAST of CSPI04G24220 vs. ExPASy TrEMBL
Match: A0A6J1IW73 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111481146 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.5e-144
Identity = 282/349 (80.80%), Postives = 298/349 (85.39%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQPHSP--LNPGFYHFPTARLSISPSDSDDQNNYWCDSP--SPNNNN----NNS 180
           VTVNQHHQ +SP  LN GFYH+P AR+SISPSDSDD  N WCDSP  S NNNN    NNS
Sbjct: 121 VTVNQHHQSYSPLGLNAGFYHYP-ARVSISPSDSDDNPN-WCDSPPLSSNNNNNNVFNNS 180

Query: 181 VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSD 240
           VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS  TSLLSD
Sbjct: 181 VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS--TSLLSD 240

Query: 241 GFPV-VRQPNHY--HHHHHHHQQVSSQI---------RNNTVGTKSFVTILEEEQQQQTK 300
           GFPV  RQPNH+   +H  + +QVS Q           NN   +KSFVTI+EE      K
Sbjct: 241 GFPVGQRQPNHHLLGYHPTNAKQVSGQTHFVGGTPNNNNNNGSSKSFVTIVEE-----PK 300

Query: 301 TKLFGVAIQSKKRLHPEYGNSNSNNNNNKARLVLEKDDLGLNLMPPSAC 330
           TKLFGV++QSKKR+HPEYG++N    NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 TKLFGVSLQSKKRVHPEYGSNNIAKENNKARLVLEKDDLGLNLMPPSAC 340

[Close]
BLAST of CSPI04G24220 vs. NCBI nr
Match: XP_004141262.2 (heat stress transcription factor B-4 [Cucumis sativus] >KAE8649863.1 hypothetical protein Csa_012628 [Cucumis sativus])

HSP 1 Score: 674.9 bits (1740), Expect = 3.6e-190
Identity = 328/329 (99.70%), Postives = 329/329 (100.00%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNSVTALSEDN 180
           VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNSVTALSEDN
Sbjct: 121 VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNSVTALSEDN 180

Query: 181 ERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQP 240
           ERLRRSNNMLMSELAH+KKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQP
Sbjct: 181 ERLRRSNNMLMSELAHIKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQP 240

Query: 241 NHYHHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQTKTKLFGVAIQSKKRLHPEYGN 300
           NHYHHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQTKTKLFGVAIQSKKRLHPEYGN
Sbjct: 241 NHYHHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQTKTKLFGVAIQSKKRLHPEYGN 300

Query: 301 SNSNNNNNKARLVLEKDDLGLNLMPPSAC 330
           SNSNNNNNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 SNSNNNNNKARLVLEKDDLGLNLMPPSAC 329

[Close]
BLAST of CSPI04G24220 vs. NCBI nr
Match: XP_008452568.1 (PREDICTED: heat stress transcription factor B-4 [Cucumis melo] >KAA0064394.1 heat stress transcription factor B-4 [Cucumis melo var. makuwa] >TYK20192.1 heat stress transcription factor B-4 [Cucumis melo var. makuwa])

HSP 1 Score: 626.3 bits (1614), Expect = 1.5e-175
Identity = 318/332 (95.78%), Postives = 321/332 (96.69%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD+CDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDSCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSP-NNNNNNSVTALSED 180
           VTVNQHHQ  SPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSP NNNNNNSVTALSED
Sbjct: 121 VTVNQHHQSLSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180

Query: 181 NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ 240
           NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST+TSLLSDGFPVVRQ
Sbjct: 181 NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSTSLLSDGFPVVRQ 240

Query: 241 PNHYHHHHHHHQQVSSQIRNN-TVGTKSFVTILEEE-QQQQTKTKLFGVAIQSKKRLHPE 300
           PNHYHHHH    QVSSQIRNN TVGTKSFVTILEEE QQQQ KTKLFGVAIQSKKRLHPE
Sbjct: 241 PNHYHHHH----QVSSQIRNNTTVGTKSFVTILEEEQQQQQPKTKLFGVAIQSKKRLHPE 300

Query: 301 YGNSNSNNNNNKARLVLEKDDLGLNLMPPSAC 330
           YGNS+S NNNNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 YGNSHS-NNNNKARLVLEKDDLGLNLMPPSAC 327

[Close]
BLAST of CSPI04G24220 vs. NCBI nr
Match: XP_038897232.1 (heat stress transcription factor B-4-like [Benincasa hispida])

HSP 1 Score: 558.5 bits (1438), Expect = 3.8e-155
Identity = 296/359 (82.45%), Postives = 307/359 (85.52%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQPHSPL---NPGFYHFPTARLSISPSDSDDQNNYWCDSPS-----PNNNNNNS 180
           VTVNQHHQ HSPL   NPGFYHFP  R+SISPSDSDDQNN WCDSPS     P+NNNNNS
Sbjct: 121 VTVNQHHQSHSPLGINNPGFYHFP-GRVSISPSDSDDQNN-WCDSPSLSSPAPHNNNNNS 180

Query: 181 VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSD 240
           VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS TTSLLSD
Sbjct: 181 VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS-TTSLLSD 240

Query: 241 GFPVVRQ--PNHYHHHHHHHQQV------------------SSQIRNNTVGTKSFVTILE 300
            FPVV+Q  PNH+HHH+H   Q+                  ++ I NN  GTKSFVTILE
Sbjct: 241 RFPVVQQRPPNHHHHHYHQQSQIHLVTGTTPTNNNNNHNNNNNIIINNNSGTKSFVTILE 300

Query: 301 EEQQQQTKTKLFGVAIQSKKRLHPEYGNSN--SNNNNNKARLVLEKDDLGLNLMPPSAC 330
           E   Q TKTKLFGVAIQSKKRLHPEYG++N    NNNNKARLVLE DDLGLNLMPPS C
Sbjct: 301 E---QPTKTKLFGVAIQSKKRLHPEYGSNNIGKENNNNKARLVLENDDLGLNLMPPSTC 353

[Close]
BLAST of CSPI04G24220 vs. NCBI nr
Match: XP_022940239.1 (heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523.1 bits (1346), Expect = 1.8e-144
Identity = 282/346 (81.50%), Postives = 298/346 (86.13%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQPHSP--LNPGFYHFPTARLSISPSDSDDQNNYWCDSP--SPNNNN---NNSV 180
           VTVNQHHQ +SP  LN GFYH+P AR+SISPSDSDD  N WCDSP  S NNNN   NNSV
Sbjct: 121 VTVNQHHQSYSPLGLNAGFYHYP-ARVSISPSDSDDNPN-WCDSPPLSSNNNNNNFNNSV 180

Query: 181 TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDG 240
           TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS  TSLLSDG
Sbjct: 181 TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS--TSLLSDG 240

Query: 241 FPV-VRQPNHY--HHHHHHHQQVSSQI-------RNNTVGTKSFVTILEEEQQQQTKTKL 300
           FPV  RQPNH+   +H  + +QVS Q         NN   +KSFVTI+EE      KTKL
Sbjct: 241 FPVGQRQPNHHLLGYHPTNAKQVSGQTHFVAGTPNNNNGSSKSFVTIVEE-----PKTKL 300

Query: 301 FGVAIQSKKRLHPEYGNSNSNNNNNKARLVLEKDDLGLNLMPPSAC 330
           FGV++QSKKR+HPEYG++N    NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 FGVSLQSKKRVHPEYGSNNIVKENNKARLVLEKDDLGLNLMPPSAC 337

[Close]
BLAST of CSPI04G24220 vs. NCBI nr
Match: XP_022982267.1 (heat stress transcription factor B-4-like [Cucurbita maxima])

HSP 1 Score: 522.3 bits (1344), Expect = 3.0e-144
Identity = 282/349 (80.80%), Postives = 298/349 (85.39%), Query Frame = 0 

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQPHSP--LNPGFYHFPTARLSISPSDSDDQNNYWCDSP--SPNNNN----NNS 180
           VTVNQHHQ +SP  LN GFYH+P AR+SISPSDSDD  N WCDSP  S NNNN    NNS
Sbjct: 121 VTVNQHHQSYSPLGLNAGFYHYP-ARVSISPSDSDDNPN-WCDSPPLSSNNNNNNVFNNS 180

Query: 181 VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSD 240
           VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS  TSLLSD
Sbjct: 181 VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS--TSLLSD 240

Query: 241 GFPV-VRQPNHY--HHHHHHHQQVSSQI---------RNNTVGTKSFVTILEEEQQQQTK 300
           GFPV  RQPNH+   +H  + +QVS Q           NN   +KSFVTI+EE      K
Sbjct: 241 GFPVGQRQPNHHLLGYHPTNAKQVSGQTHFVGGTPNNNNNNGSSKSFVTIVEE-----PK 300

Query: 301 TKLFGVAIQSKKRLHPEYGNSNSNNNNNKARLVLEKDDLGLNLMPPSAC 330
           TKLFGV++QSKKR+HPEYG++N    NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 TKLFGVSLQSKKRVHPEYGSNNIAKENNKARLVLEKDDLGLNLMPPSAC 340

[Close]
BLAST of CSPI04G24220 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 322.4 bits (825), Expect = 4.2e-88
Identity = 185/331 (55.89%), Postives = 228/331 (68.88%), Query Frame = 0 

Query: 19  KAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 78
           KA+PAPFLTKTYQLVDDP+TDH+VSWG+DDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 79  QLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ--PQ----VTVNQHHQPHSP 138
           QLNTYGFRKIVPDRWEFANEFF++GEKHLLCEIHRRKT+Q  PQ       + H  P  P
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMSHHHAPPQIP 148

Query: 139 LNPG-FYHFPTARLSISPSDSDDQNNYWCDSPSPNN--------NNNNSVTALSEDNERL 198
            + G F+  P  R++     + ++++YWCD   P+         +    VTALSEDNERL
Sbjct: 149 FSGGSFFPLPPPRVT-----TPEEDHYWCDDSPPSRPRVIPQQIDTAAQVTALSEDNERL 208

Query: 199 RRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQPNHY 258
           RRSN +LMSELAHMKKLYNDIIYFVQNHVKPVAPSN+  Y   +S L            Y
Sbjct: 209 RRSNTVLMSELAHMKKLYNDIIYFVQNHVKPVAPSNNSSY--LSSFLQKQQQQQPPTLDY 268

Query: 259 HHHHHHHQQVSSQIRNNTVGTKSFVTILEE------EQQQQTKTKLFGVAI-QSKKRLHP 318
           ++    +    + + ++   ++S +T+LE+      +Q    KTKLFGV++  SKKR H 
Sbjct: 269 YNTATVNATNLNALNSSPPTSQSSITVLEDDHTNHHDQSNMRKTKLFGVSLPSSKKRSH- 328

Query: 319 EYGNSNSNNNNNKARLVLEKDDLGLNLMPPS 328
                + ++  +K RLVL++ DL LNLM  S
Sbjct: 329 -----HFSDQTSKTRLVLDQSDLALNLMTAS 346

[Close]
BLAST of CSPI04G24220 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 183.7 bits (465), Expect = 2.4e-46
Identity = 103/229 (44.98%), Postives = 137/229 (59.83%), Query Frame = 0 

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           S ++IP PFLTKT+ LV+D S D ++SW ED ++F+VW P +FA+DLLP +FKHNNFSSF
Sbjct: 16  SQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSF 75

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTV-----NQHHQPH- 136
           VRQLNTYGF+K+VPDRWEF+N+FF++GEK LL EI RRK      TV      Q +Q   
Sbjct: 76  VRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITTTHQTVVAPSSEQRNQTMV 135

Query: 137 -SPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNSVTALSEDNERLRRSNNM 196
            SP N G  +     +S SPS      +++C       N   SV  L E+NE+LR  N  
Sbjct: 136 VSPSNSGEDNNNNQVMSSSPS------SWYCHQTKTTGNGGLSVELL-EENEKLRSQNIQ 195

Query: 197 LMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVR 239
           L  EL  MK + ++I   + N+V       SY    ++S   +  P  R
Sbjct: 196 LNRELTQMKSICDNIYSLMSNYVGSQPTDRSYSPGGSSSQPMEFLPAKR 237

[Close]
BLAST of CSPI04G24220 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 179.9 bits (455), Expect = 3.4e-45
Identity = 99/205 (48.29%), Postives = 128/205 (62.44%), Query Frame = 0 

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           + +++PAPFL+KTYQLVDD STD +VSW E+ T FVVW+  EFA+DLLP YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRK-----TAQPQVTVNQHHQPHS 136
           +RQLNTYGFRK VPD+WEFAN++FR+G + LL +I RRK     TA   V V    + +S
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTAGKCVVVGSPSESNS 126

Query: 137 PLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNN---NNSVTALSEDNERLRRSNN 196
                                DD  +    SP  + N     N V  LS +NE+L+R NN
Sbjct: 127 ------------------GGGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENN 186

Query: 197 MLMSELAHMKKLYNDIIYFVQNHVK 214
            L SELA  KK  ++++ F+  H+K
Sbjct: 187 NLSSELAAAKKQRDELVTFLTGHLK 193

[Close]
BLAST of CSPI04G24220 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 174.9 bits (442), Expect = 1.1e-43
Identity = 119/314 (37.90%), Postives = 164/314 (52.23%), Query Frame = 0 

Query: 16  DSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSS 75
           DS ++IP PFLTKTYQLV+DP  D ++SW ED TTF+VWRP EFARDLLP YFKHNNFSS
Sbjct: 51  DSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSS 110

Query: 76  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQPHSPLNP 135
           FVRQLNTYGFRK+VPDRWEF+N+ F++GEK LL +I RRK +QP +         +    
Sbjct: 111 FVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAAAAVAAS 170

Query: 136 G--FYHFPTARLSISPSDSDDQNNYWCDSPSP-----------------NNNNNNSVTAL 195
                  P     +SPS+S ++     +S SP                    +  +   L
Sbjct: 171 AVTVAAVPVVAHIVSPSNSGEEQVISSNS-SPAAAAAAIGGVVGGGSLQRTTSCTTAPEL 230

Query: 196 SEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPV 255
            E+NERLR+ N  L  E+  +K LY +I   + N                  LL +G P+
Sbjct: 231 VEENERLRKDNERLRKEMTKLKGLYANIYTLMANFTP--------GQEDCAHLLPEGKPL 290

Query: 256 VRQPNHYHHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQTKTKLFGVAIQSKK-RLH 310
              P          Q++S  I  + + T   + + E+        +LFGV+I  K+ R  
Sbjct: 291 DLLP--------ERQEMSEAIMASEIETGIGLKLGED-----LTPRLFGVSIGVKRARRE 342

[Close]
BLAST of CSPI04G24220 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 159.5 bits (402), Expect = 4.8e-39
Identity = 84/180 (46.67%), Postives = 112/180 (62.22%), Query Frame = 0 

Query: 20  AIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQ 79
           ++P PFL+KTY +V+DP+TD IVSW   + +F+VW PPEF+RDLLP YFKHNNFSSFVRQ
Sbjct: 48  SLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQ 107

Query: 80  LNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQPHSPLNPGFYH 139
           LNTYGFRK+ PDRWEFANE F +G+KHLL +I RRK+ Q   +        S  NP    
Sbjct: 108 LNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGS--------SSSNPQSQQ 167

Query: 140 FPTARLSISPSDSDDQNNYWCDSPSPNNNNNNSVTALSEDNERLRRSNNMLMSELAHMKK 199
               + S++   S  +   +                L E+ E+L+R  N+LM EL  +++
Sbjct: 168 LSQGQGSMAALSSCVEVGKF---------------GLEEEVEQLKRDKNVLMQELVKLRQ 204

The following BLAST results are available for this feature:
BLAST of CSPI04G24220 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9C6356.0e-8755.89Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
Q7XHZ01.9e-6954.90Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q10KX81.7e-6559.07Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q67U947.1e-6442.97Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9R85.8e-5853.42Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
BLAST of CSPI04G24220 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L4W86.0e-191100.00HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 ... [more]
A0A5A7VAY17.1e-17695.78Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BUY57.1e-17695.78heat stress transcription factor B-4 OS=Cucumis melo OX=3656 GN=LOC103493553 PE=... [more]
A0A6J1FJH28.5e-14581.50heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IW731.5e-14480.80heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
BLAST of CSPI04G24220 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004141262.23.6e-19099.70heat stress transcription factor B-4 [Cucumis sativus] >KAE8649863.1 hypothetica... [more]
XP_008452568.11.5e-17595.78PREDICTED: heat stress transcription factor B-4 [Cucumis melo] >KAA0064394.1 hea... [more]
XP_038897232.13.8e-15582.45heat stress transcription factor B-4-like [Benincasa hispida][more]
XP_022940239.11.8e-14481.50heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 h... [more]
XP_022982267.13.0e-14480.80heat stress transcription factor B-4-like [Cucurbita maxima][more]
BLAST of CSPI04G24220 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G46264.14.2e-8855.89heat shock transcription factor B4 [more]
AT5G62020.12.4e-4644.98heat shock transcription factor B2A [more]
AT4G36990.13.4e-4548.29heat shock factor 4 [more]
AT4G11660.11.1e-4337.90winged-helix DNA-binding transcription factor family protein [more]
AT4G17750.14.8e-3946.67heat shock factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 173..193
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 149..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 149..177
NoneNo IPR availablePANTHERPTHR10015:SF382BNAC06G00310D PROTEINcoord: 12..327
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 76..88
score: 84.62
coord: 25..48
score: 47.38
coord: 63..75
score: 78.68
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 21..114
e-value: 5.1E-60
score: 215.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 25..114
e-value: 2.4E-32
score: 111.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 64..88
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..116
e-value: 1.1E-37
score: 130.6
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 12..327
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 22..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G24220.1CSPI04G24220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding