CSPI04G24120 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G24120
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTrimethylguanosine synthase
LocationChr4: 22006445 .. 22012448 (+)
RNA-Seq ExpressionCSPI04G24120
SyntenyCSPI04G24120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATTCGGATTCTGAAAGTACGAGTCCGTTGCACCGCCGAATGAGGGTTGCTTTATTTTAGACTTTTAGCAAAGCTTCGAAAAATGGCGGTAGAAAGTGTGCCCTCGTAAACACTGGTTGTAGTTCTTGATCGGAGTTTCCTTTTCTGCTGACACGATGGGGTCTTGCAACGAAGAATCTGAAGATGAACCTGGAGTCTCTCCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACCGAAGTCTTTCTCTGGTAACCACTCTTTCTCACCTCCATTATCGCCGAAAGATTAGGGAAATATAAACTAGAAGAAAACAGCTGATTTTACTTTGAACACAATGCTCTGTTTTACTCTTCTTCTTTTTCTTTTTGATTGTTTTGGTATTGACGATAATTTTAATGGTTGCGTTACAGGGACGAGGAGACGGAAGTAGCTAGACGAGTGGAAAGTCGTGTAAGTCTCTAATTTTGTCACTGGTTCAAATTGCGGCATTGATAATTATTGAATTCTCTTTGCGCCTTGGTTACTTTCCATCCAATCTCTACTAATTTCTTTGTTTGTTTTAACAAGTTAGCTCTTGATGCTGATGATGCCAATAACGGAAAATCCGTGGAGAAAATCTGTTCTACAATCAGTGGCAAGTTTTTCATTCATTATCTCTTTAATTATTATTTTAAGTTCTGAGTTATCTGTGGCATAATCATGCATGTTGATTGGTGCTAAAGAGTCAGGATTGCTTTTCTTCGGGCAGGTATCAGCTTATTACCGGAGGATATCGAACTTACTGAACAGATGAATGCTTTGGGCCTTCCTCTTTCATTCCACACAAACAAAGAAGTAATTCAACATACTACTTCTGTACATTACTTAGGTTAAATTATGATATTCCTCCTGTAGTCAACATGCTGACATTCATATACCTCCAGAAGTGTATGTTTGATTAATTAATTTCAACGGTCTTTATCTTTAGTGATTGTTAAAAAACGGTGAACTACATTGCCAAGTTAGTCTACAGCAATGTTGTGTCAATTCTACATTAGTACCACGTTAAGGTATAGTTTAGCCCATTACATAAATAAGCTATTGCACATTTGTTTCATCCATTATTTTGGCCATATACTCTTACAATTATTGCTTATTGGAAATTATCATACAGTAAGTGTAATATGTGTTCTGTTGCATTTCTTCTAGAACAGTTGCAAGTATTAAGATGAGATTCACATATCTATTGCATTACGAGTATGGCATACTCAGCATCATTTCTCTTTCAAGATGTTGAGGGGTTGGCTTATACATGGCATGGAAATATGAGTTTTCTGGAAGAAGAATAGCTAAATGGGTTGAAATGTTTTTGTGAATTACAATAACACCATTTTGTTGGAAATGATTTTCCCCCTGAATTTATCCTTAGTTGTGATGTTTTGGCATGCAGCAGAAGAGAATTGGAATTACCATGGTTAAAAGAAAAGCAAATGTCAAGCACTCTAGAATTCAGCAAGGATTTCTAGACAAAGAAGTGGAGTTTCCTAAAGCGAGCAGCAGGGAGGAGATTGTAGCTAATAATACTTTCAATGATGACGCAACAGGTTCTCTATGCAGCTATTCTATGGTGAATCAAAGTGAGACTTCTGATCGTGATGTGGTGTTGGACACCAATGAAATCCATGTTATCTTTGATGGAGATATTTCACGTAATTCAAGTGGAGTTATCAGTGGTGCTGTTGAAGAACAATTTTGTGATGTTATGTGTGATATTGTGCTGAATAATGGGGGAGACCATGAATTAAGCTCAGACGATGCTGTGCTGGGGGATCATACAAAAGTTAGATTGAGTTCAATTGGTTTTGATAAGGGTTATTCTCCAAGACTGCGTACCACAGGTCTTGATGTCGGCCATGGCAAGCAGGAGGAAGTTGAACCACCTATGGAGTCAGAAGGTTCTTCCACAACTTTCCAAGATACTGAAGTGCAGAAGAGTGACACTGATAGTGGCATTGGACTACCAGAAGTAGCTGAACCATGTTTCCTTCGTATGGAACCGGACTGTAATGAAAATGACCAAGTTGTTGGATGTATTCATGAATCTGGAGACTGGATGGTGTACTGGGATTCTTTTTATATGAGAAACTACTTCTACAATATAAAGTCACATGAGTCTACTTGGAATCCTCCTTTAGGGTTGGAACATTTTGCATCATCTGATGCCAATTTCACTCCTAATGAATCAACTGCCGAAGTTTGTGAAATGGATGTATTAGAAGATGTAAAATCAGAAGATATTTGTAGGGTGCTCGGTGATACCGAGTGTATGAATTTACTTGGTGATAGTGTTCACTGTCAACCACCTGATGCACTGTTGGAGGGCTCTAGTAGTTTAATTGAGGGCATCGAAAGTAGTGCATTTATCGATACTTCAATTAACTGCTCTAAGGATGAACCTCAAGAGTGGCTGATGAGTTGTAGAAATACCAGGGAAAATATTGGATGCAGGTTCTTGACCAGTCTATGATTTCTGTTCCTAATTTTTTTTCTCTTTTACCTATTCGACTAATTTGTTTGGAGAGGAATATTGATTAGTATTGTGGTTTCAGTTGTGAAGGTCATGCCAAGCAATCATGTGGTGAGAATTGCACCAATGGTTCCCAGTTCATTGCTGCAAATGGAGCTTCTGAACAAATGATGTTCAGCCATCACAAACCTAGTAACATGCACTCACCCGAGATAGATTGCATCACCATAGACGACGATGAAGGCACAGCAGGCTTAACTACTAGCAGGTACCATTTAGAATCAGAATTGTGACTGGACCTATTAGCTTTCTTATCAGACCACAAATTTCTCTAATGTTTGGGCTCCCTTGCTGTGTTTCCAGTGTTTCTCATATGCTTCAGCAGGCAGATCATATCGATGGTGATATGCATTTTGCAAATGGACCTATCATTTGTACACTGGGTACTGTGCAAAATCTTTCTGTAAGAAATAGGAAAAGGAAAATGAAAAGAACACGCAGACGTGGACAATTATCTGATAGAAATGAAGGTTTAATTTTCTGCCTTCACCGTGCCTGCAAAATTATTAGTTTTGACATCAGTAAACATTTTATTCACATTCGACTCCATAAAGTTTCTCCAGTTAGGTCTTCTTGCCAGCATAAAGAGGAATATCAAATGATTCTGGCAGAAGTAAATAGACAGAAAGGTTAATTTGTAGATTGGTTAATTATTAAAATATGGCACGTTTCCTTTGATTGCAACCTTTTCTATGGTACAATACATTGGTTCTATTCACAATTATTTTTGGTCTGAAGATTGAAGATCATATGAACTTGGCACTCCGGTTAAAAGTTTAGATTATTCCTTAACATATGGGTTGTAAAATTAGTAACTGTACCTGCAAAATTAGTGGTTTGGTGCCTGCATCAGGATTATAAACCTTATTTAGTGCTTCAACAATGTGTCTGACGAGTATGATATATGACCAATTTTGGCTGGTGGTGCTTTCTGGTTGTAACTCATAAAACAATTGCTACTGTCAAATTAGTCTCTCTTGTTAGCAGAAAAGATTTCTTAGATGGGCTCATGTTCGAAATAGATAATGAAATGCCATAAGATTCTTTTCTCCCTAACAGACTAGTTTTCTTATGCAGGATTTCGTTCTTTTGCAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCAACTTTTCTCCAGATTTGATGATGGTATAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCTATAGCTAGGCATCACGCCTCACGTTGTGGTAGCAATATGATCATTGACGGTTTCACCGGTGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGTGATGTTTGGTAATCTTTTCTTTGTCCATCTTCATAAGTTTTCAGGGACAATTAACCCTTTCGTGTATCTTATAAGCTCCGGTAATTGGTTAGGATATTGAGTAGTTTATTTTTCATTTGTATCAGTGGTTAATGAATAAGTGGTTCTGATCCAATTTGATATGTTATTCTCTTTTACAATTTCTATAAGATGTTTATTAGAATATTCCTGACAACATCTTTGTTTAATTAATGTTAATGATATTGCAGGGCCAAACATGTTATTGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCTGCTATATATGGTGTTGAAGATCAAATAGATTTTCTAAAGGGGGACTTCTTCCGTTTAGCCCCACACCTCAAGGTATTGACCCTTCTAGAATTTTTTTTTCTTTTTTTTTCATGTACATTCAAAATTCTCTTGCTTCCGGTGCTGAGAATCAGCATTATCAACGTTTGCACTTTAGATAGTGACAGGTAGTTCATTGTCAGGAGAAGGTATTATCTTAAGTATCGTCGTTATATCTGCCTGAATGATTTGAAACTGTCATAAGATTATCAATTTTTCTTACCAGTAATATAGTGAATGCAATTAATTTTGGCATAAGTTGGAATTTTTTGGAGGACAAGTCCAGAATTTTGTGCATCTGTCTTTTGAATTGTGGCTTTGATCTTGCATATTCTATAATTCACTTTTTTAAATTATACAAAAAATTAAACTTTCTATGCTATACTTCCATCTCACTTTTCACTATACTAGAATGTTATCGGATTCTACCTGCTGCAGGCTGACGTTATATTCTTATCACCTCCTTGGGGAGGCCCTGATTATGCCGGTGTAGATATTTATGACCTGACCAAGCTCAAACCACATGATGGGTAAGCTATCTGACTTTGATAGTTCGTGGCGAGTTCAGGTTGCCCTACTATTATTTTTACCATCCTCTGAGCTTTGCTTTTTGCCTGTACCTATGCAGGTATTTTCTCTTCAACGTTGCTAAGAAAATTGCTCCCCTCGTTGTCATGTTTCTTCCAAAAAATGTTAATCTCAACCAACTAGCGGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTAAGAATATTCTTTTTGTTTTCAATATTCGTTCTTGTGAATTCTTGGGATGCTTAACAATTGACACTTAGTTATTGAGGTTGGTTTGGTGCTTTTTGTGGAACAAAAGAATATTAACAATTGCAGAGTTAAGGCAGTAGTTCCTATTGTAATTTTGCATAGCTTATGAGTACTCACCTTGTCTCTCATTACATGCAAACGCTTGCAATTTCAAACCATTGACCAATAATTCATGCATTTGCATTCAAGCTTTCACACACACTTGGATCATGAATGCATACCAACATCTACACTCCTCTCCTAGTCTGGAGAATTATAACTTCTTCTTTTTTTCCCTCCCTTTTCGATGCTATTTTCCCTATAACACAAAAGTCCTTCCAGAATATTGCATCTTACAACTTATTTTAAATTCTAATTATTTGCAATTGTGTTCGTAGGTTGAGAAAAACTTTTTAAATGGCAAGTTGAAGGCGATTACTGCTTACCTTAGCAATGGCTCCATAAACAAAGACAATGTCACCTAATGCGTGATATCATATACTCCTTTTGAGAAATTCTCCAGCAATTTTGATGGCTAAGGTATAAAATGAAGTTGGTTGTTTCAGTTTATAGTTATATCCTAATGGGTATAAGGTATAAAATGAAGTCGCCCTGTATTTTCTCTTGGAACATTATATTAAACTAATGAAACCATTTAAGTTGTCGTTTTTGCTGGCAAAACAGATGCTTAATTTATTGGCTTGACAAAACATTTTGGCGGACATGGAGGAATAGTTCATTGTACAGTCAACAAAACTCTTATAATGGTGAGACCCTCTTACATTACCAGAAAATGGTTCACAGCTGGTTGGGGAAAAACATTTAATTTTTGTGGTTGTATTTATGAATGTACTGGACCCACTAAGAGGAAGAAGGAAAAAGAAAAAGAAAAAGAAAAGAACACCATGACAACTAGTAACTTTGTAAACAATATGATGTTAAATCAGTCTCAAATTTGGGGTTT

mRNA sequence

GTATTCGGATTCTGAAAGTACGAGTCCGTTGCACCGCCGAATGAGGGTTGCTTTATTTTAGACTTTTAGCAAAGCTTCGAAAAATGGCGGTAGAAAGTGTGCCCTCGTAAACACTGGTTGTAGTTCTTGATCGGAGTTTCCTTTTCTGCTGACACGATGGGGTCTTGCAACGAAGAATCTGAAGATGAACCTGGAGTCTCTCCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACCGAAGTCTTTCTCTGGGACGAGGAGACGGAAGTAGCTAGACGAGTGGAAAGTCGTTTAGCTCTTGATGCTGATGATGCCAATAACGGAAAATCCGTGGAGAAAATCTGTTCTACAATCAGTGGTATCAGCTTATTACCGGAGGATATCGAACTTACTGAACAGATGAATGCTTTGGGCCTTCCTCTTTCATTCCACACAAACAAAGAAAAGAGAATTGGAATTACCATGGTTAAAAGAAAAGCAAATGTCAAGCACTCTAGAATTCAGCAAGGATTTCTAGACAAAGAAGTGGAGTTTCCTAAAGCGAGCAGCAGGGAGGAGATTGTAGCTAATAATACTTTCAATGATGACGCAACAGGTTCTCTATGCAGCTATTCTATGGTGAATCAAAGTGAGACTTCTGATCGTGATGTGGTGTTGGACACCAATGAAATCCATGTTATCTTTGATGGAGATATTTCACGTAATTCAAGTGGAGTTATCAGTGGTGCTGTTGAAGAACAATTTTGTGATGTTATGTGTGATATTGTGCTGAATAATGGGGGAGACCATGAATTAAGCTCAGACGATGCTGTGCTGGGGGATCATACAAAAGTTAGATTGAGTTCAATTGGTTTTGATAAGGGTTATTCTCCAAGACTGCGTACCACAGGTCTTGATGTCGGCCATGGCAAGCAGGAGGAAGTTGAACCACCTATGGAGTCAGAAGGTTCTTCCACAACTTTCCAAGATACTGAAGTGCAGAAGAGTGACACTGATAGTGGCATTGGACTACCAGAAGTAGCTGAACCATGTTTCCTTCGTATGGAACCGGACTGTAATGAAAATGACCAAGTTGTTGGATGTATTCATGAATCTGGAGACTGGATGGTGTACTGGGATTCTTTTTATATGAGAAACTACTTCTACAATATAAAGTCACATGAGTCTACTTGGAATCCTCCTTTAGGGTTGGAACATTTTGCATCATCTGATGCCAATTTCACTCCTAATGAATCAACTGCCGAAGTTTGTGAAATGGATGTATTAGAAGATGTAAAATCAGAAGATATTTGTAGGGTGCTCGGTGATACCGAGTGTATGAATTTACTTGGTGATAGTGTTCACTGTCAACCACCTGATGCACTGTTGGAGGGCTCTAGTAGTTTAATTGAGGGCATCGAAAGTAGTGCATTTATCGATACTTCAATTAACTGCTCTAAGGATGAACCTCAAGAGTGGCTGATGAGTTGTAGAAATACCAGGGAAAATATTGGATGCAGTTGTGAAGGTCATGCCAAGCAATCATGTGGTGAGAATTGCACCAATGGTTCCCAGTTCATTGCTGCAAATGGAGCTTCTGAACAAATGATGTTCAGCCATCACAAACCTAGTAACATGCACTCACCCGAGATAGATTGCATCACCATAGACGACGATGAAGGCACAGCAGGCTTAACTACTAGCAGTGTTTCTCATATGCTTCAGCAGGCAGATCATATCGATGGTGATATGCATTTTGCAAATGGACCTATCATTTGTACACTGGGTACTGTGCAAAATCTTTCTGTAAGAAATAGGAAAAGGAAAATGAAAAGAACACGCAGACGTGGACAATTATCTGATAGAAATGAAGGATTTCGTTCTTTTGCAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCAACTTTTCTCCAGATTTGATGATGGTATAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCTATAGCTAGGCATCACGCCTCACGTTGTGGTAGCAATATGATCATTGACGGTTTCACCGGTGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGGCCAAACATGTTATTGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCTGCTATATATGGTGTTGAAGATCAAATAGATTTTCTAAAGGGGGACTTCTTCCGTTTAGCCCCACACCTCAAGGCTGACGTTATATTCTTATCACCTCCTTGGGGAGGCCCTGATTATGCCGGTGTAGATATTTATGACCTGACCAAGCTCAAACCACATGATGGGTATTTTCTCTTCAACGTTGCTAAGAAAATTGCTCCCCTCGTTGTCATGTTTCTTCCAAAAAATGTTAATCTCAACCAACTAGCGGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTTGAGAAAAACTTTTTAAATGGCAAGTTGAAGGCGATTACTGCTTACCTTAGCAATGGCTCCATAAACAAAGACAATGTCACCTAATGCGTGATATCATATACTCCTTTTGAGAAATTCTCCAGCAATTTTGATGGCTAAGATGCTTAATTTATTGGCTTGACAAAACATTTTGGCGGACATGGAGGAATAGTTCATTGTACAGTCAACAAAACTCTTATAATGGTGAGACCCTCTTACATTACCAGAAAATGGTTCACAGCTGGTTGGGGAAAAACATTTAATTTTTGTGGTTGTATTTATGAATGTACTGGACCCACTAAGAGGAAGAAGGAAAAAGAAAAAGAAAAAGAAAAGAACACCATGACAACTAGTAACTTTGTAAACAATATGATGTTAAATCAGTCTCAAATTTGGGGTTT

Coding sequence (CDS)

ATGGGGTCTTGCAACGAAGAATCTGAAGATGAACCTGGAGTCTCTCCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACCGAAGTCTTTCTCTGGGACGAGGAGACGGAAGTAGCTAGACGAGTGGAAAGTCGTTTAGCTCTTGATGCTGATGATGCCAATAACGGAAAATCCGTGGAGAAAATCTGTTCTACAATCAGTGGTATCAGCTTATTACCGGAGGATATCGAACTTACTGAACAGATGAATGCTTTGGGCCTTCCTCTTTCATTCCACACAAACAAAGAAAAGAGAATTGGAATTACCATGGTTAAAAGAAAAGCAAATGTCAAGCACTCTAGAATTCAGCAAGGATTTCTAGACAAAGAAGTGGAGTTTCCTAAAGCGAGCAGCAGGGAGGAGATTGTAGCTAATAATACTTTCAATGATGACGCAACAGGTTCTCTATGCAGCTATTCTATGGTGAATCAAAGTGAGACTTCTGATCGTGATGTGGTGTTGGACACCAATGAAATCCATGTTATCTTTGATGGAGATATTTCACGTAATTCAAGTGGAGTTATCAGTGGTGCTGTTGAAGAACAATTTTGTGATGTTATGTGTGATATTGTGCTGAATAATGGGGGAGACCATGAATTAAGCTCAGACGATGCTGTGCTGGGGGATCATACAAAAGTTAGATTGAGTTCAATTGGTTTTGATAAGGGTTATTCTCCAAGACTGCGTACCACAGGTCTTGATGTCGGCCATGGCAAGCAGGAGGAAGTTGAACCACCTATGGAGTCAGAAGGTTCTTCCACAACTTTCCAAGATACTGAAGTGCAGAAGAGTGACACTGATAGTGGCATTGGACTACCAGAAGTAGCTGAACCATGTTTCCTTCGTATGGAACCGGACTGTAATGAAAATGACCAAGTTGTTGGATGTATTCATGAATCTGGAGACTGGATGGTGTACTGGGATTCTTTTTATATGAGAAACTACTTCTACAATATAAAGTCACATGAGTCTACTTGGAATCCTCCTTTAGGGTTGGAACATTTTGCATCATCTGATGCCAATTTCACTCCTAATGAATCAACTGCCGAAGTTTGTGAAATGGATGTATTAGAAGATGTAAAATCAGAAGATATTTGTAGGGTGCTCGGTGATACCGAGTGTATGAATTTACTTGGTGATAGTGTTCACTGTCAACCACCTGATGCACTGTTGGAGGGCTCTAGTAGTTTAATTGAGGGCATCGAAAGTAGTGCATTTATCGATACTTCAATTAACTGCTCTAAGGATGAACCTCAAGAGTGGCTGATGAGTTGTAGAAATACCAGGGAAAATATTGGATGCAGTTGTGAAGGTCATGCCAAGCAATCATGTGGTGAGAATTGCACCAATGGTTCCCAGTTCATTGCTGCAAATGGAGCTTCTGAACAAATGATGTTCAGCCATCACAAACCTAGTAACATGCACTCACCCGAGATAGATTGCATCACCATAGACGACGATGAAGGCACAGCAGGCTTAACTACTAGCAGTGTTTCTCATATGCTTCAGCAGGCAGATCATATCGATGGTGATATGCATTTTGCAAATGGACCTATCATTTGTACACTGGGTACTGTGCAAAATCTTTCTGTAAGAAATAGGAAAAGGAAAATGAAAAGAACACGCAGACGTGGACAATTATCTGATAGAAATGAAGGATTTCGTTCTTTTGCAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCAACTTTTCTCCAGATTTGATGATGGTATAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCTATAGCTAGGCATCACGCCTCACGTTGTGGTAGCAATATGATCATTGACGGTTTCACCGGTGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGGCCAAACATGTTATTGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCTGCTATATATGGTGTTGAAGATCAAATAGATTTTCTAAAGGGGGACTTCTTCCGTTTAGCCCCACACCTCAAGGCTGACGTTATATTCTTATCACCTCCTTGGGGAGGCCCTGATTATGCCGGTGTAGATATTTATGACCTGACCAAGCTCAAACCACATGATGGGTATTTTCTCTTCAACGTTGCTAAGAAAATTGCTCCCCTCGTTGTCATGTTTCTTCCAAAAAATGTTAATCTCAACCAACTAGCGGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTTGAGAAAAACTTTTTAAATGGCAAGTTGAAGGCGATTACTGCTTACCTTAGCAATGGCTCCATAAACAAAGACAATGTCACCTAA

Protein sequence

MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVEKICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFLDKEVEFPKASSREEIVANNTFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDISRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPRLRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGIGLPEVAEPCFLRMEPDCNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNGSINKDNVT*
Homology
BLAST of CSPI04G24120 vs. ExPASy Swiss-Prot
Match: P85107 (Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 2.2e-63
Identity = 127/277 (45.85%), Postives = 177/277 (63.90%), Query Frame = 0

Query: 507 TTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQLSDRNEG 566
           T SS S +  +A+  +G    A  P  C+   V N      + ++K+ +++ +  ++N+ 
Sbjct: 568 TISSASEL--EAEKSEGGSLVAAVPENCSTEGVANSPRAEAEVEIKKKKKK-KKKNKNKK 627

Query: 567 FRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGS--- 626
                        + KYW QRY+LFSRFDDGIK+DKEGWFSVTPE IA H A R      
Sbjct: 628 INGLPPEIASVPELAKYWAQRYRLFSRFDDGIKLDKEGWFSVTPEKIAEHIAGRVSQSFN 687

Query: 627 -NMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFR 686
            ++I+D F GVGGN IQF+   K VIAIDIDP KI  A++NA +YGV D+I+F+ GDF  
Sbjct: 688 CDIIVDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARNNAEVYGVADKIEFICGDFLL 747

Query: 687 LAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNVN 746
           LAP LKADV+FLSPPWGGPDYA  + +D+  +   DG+ +F +++KI   +V FLP+N +
Sbjct: 748 LAPCLKADVVFLSPPWGGPDYATAETFDIRTMMSPDGFEIFRLSQKITNNIVYFLPRNAD 807

Query: 747 LNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSN 780
           ++Q+A L   + P   +E+E+NFLN KLK ITAY  +
Sbjct: 808 VDQVASL---AGPGGQVEIEQNFLNNKLKTITAYFGD 838

BLAST of CSPI04G24120 vs. ExPASy Swiss-Prot
Match: Q96RS0 (Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3)

HSP 1 Score: 238.8 bits (608), Expect = 2.1e-61
Identity = 110/204 (53.92%), Postives = 146/204 (71.57%), Query Frame = 0

Query: 580 ITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGS----NMIIDGFTGVGG 639
           + KYW QRY+LFSRFDDGIK+D+EGWFSVTPE IA H A R       ++++D F GVGG
Sbjct: 644 LAKYWAQRYRLFSRFDDGIKLDREGWFSVTPEKIAEHIAGRVSQSFKCDVVVDAFCGVGG 703

Query: 640 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLS 699
           N IQF+     VIAIDIDP KI  A++NA +YG+ D+I+F+ GDF  LA  LKADV+FLS
Sbjct: 704 NTIQFALTGMRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLS 763

Query: 700 PPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDP 759
           PPWGGPDYA  + +D+  +   DG+ +F ++KKI   +V FLP+N +++Q+A L   + P
Sbjct: 764 PPWGGPDYATAETFDIRTMMSPDGFEIFRLSKKITNNIVYFLPRNADIDQVASL---AGP 823

Query: 760 PWSLEVEKNFLNGKLKAITAYLSN 780
              +E+E+NFLN KLK ITAY  +
Sbjct: 824 GGQVEIEQNFLNNKLKTITAYFGD 844

BLAST of CSPI04G24120 vs. ExPASy Swiss-Prot
Match: Q923W1 (Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2)

HSP 1 Score: 237.7 bits (605), Expect = 4.6e-61
Identity = 119/256 (46.48%), Postives = 166/256 (64.84%), Query Frame = 0

Query: 528 ANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQR 587
           A  P  C+   + N      + ++K+ +++ +    N+     A   E    + KYW QR
Sbjct: 588 ATVPENCSTEEIPNSPHAETEVEIKKKKKKNKNKKINDLPPEIASVPE----LAKYWAQR 647

Query: 588 YQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGS----NMIIDGFTGVGGNAIQFSQR 647
           Y+LFSRFDDGIK+DKEGWFSVTPE IA H A R       ++++D F GVGGN IQF+  
Sbjct: 648 YRLFSRFDDGIKLDKEGWFSVTPEKIAEHIAGRVSQAFRCDVVVDAFCGVGGNTIQFALT 707

Query: 648 AKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDY 707
            K VIAIDIDP KI  A++NA +YG+ D+I+F+ GDF  LAP LKADV+FLSPPWGGPDY
Sbjct: 708 GKRVIAIDIDPVKIDLARNNAEVYGIADKIEFICGDFLLLAPCLKADVVFLSPPWGGPDY 767

Query: 708 AGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEK 767
           A  + +D+  +   DG+ +F +++KI   +V FLP+N +++Q+A L+        +E+E+
Sbjct: 768 ATAETFDIRTMMSPDGFEIFRLSQKITNNIVYFLPRNADIDQVASLAGLGG---QVEIEQ 827

Query: 768 NFLNGKLKAITAYLSN 780
           NFLN KLK ITAY  +
Sbjct: 828 NFLNNKLKTITAYFGD 836

BLAST of CSPI04G24120 vs. ExPASy Swiss-Prot
Match: Q09814 (Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tgs1 PE=1 SV=3)

HSP 1 Score: 164.1 bits (414), Expect = 6.5e-39
Identity = 90/228 (39.47%), Postives = 127/228 (55.70%), Query Frame = 0

Query: 562 DRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPE----PIARHH 621
           D +E  +   I    P ++ KYW  RY LFSRFD+GI +D + W+SVTPE     IA+  
Sbjct: 8   DEDELLKKCIICPPVPKALKKYWNNRYNLFSRFDEGIWLDYQSWYSVTPEKVAVAIAKSV 67

Query: 622 ASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGV-EDQIDFL 681
                  +IID F+G GGN IQF++    VI+I+IDP KI  A+HN  IYG+   ++ F+
Sbjct: 68  VDFIQPELIIDAFSGCGGNTIQFAKYCP-VISIEIDPIKIAMAKHNLEIYGIPSSRVTFI 127

Query: 682 KGDFFRLAPHLK-----ADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAP 741
           +GD       L+       ++F+SPPWGGP Y+G  +Y L  L P+    LF  A +I+P
Sbjct: 128 QGDVLDTFKSLQFAKDYRSLVFMSPPWGGPSYSGKTVYSLNDLNPYAFDVLFKEATRISP 187

Query: 742 LVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFL-NGKLKAITAYLS 779
            V  FLP+N ++ +LA      + P+      NFL  G  KAI  Y +
Sbjct: 188 YVAAFLPRNTDVKELAAYGSIHNKPYI----TNFLFEGYAKAICCYFN 230

BLAST of CSPI04G24120 vs. ExPASy Swiss-Prot
Match: Q12052 (Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGS1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 3.3e-27
Identity = 74/178 (41.57%), Postives = 99/178 (55.62%), Query Frame = 0

Query: 582 KYWCQRYQLFSRFDD-GIKMDKEGWFSVTPEPIA---RHHASRCGSN--MIIDGFTGVGG 641
           KYW  R +LFS+ D   I M  E WFSVTPE IA    +    C  N   I+D F G GG
Sbjct: 51  KYWKNRRRLFSKIDSASIYMTDELWFSVTPERIACFLANFVKACMPNAERILDVFCGGGG 110

Query: 642 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLA-----PHLKAD 701
           N IQF+ +  +V  +D     I     NA  YGV+D+I   +G + +L        +K D
Sbjct: 111 NTIQFAMQFPYVYGVDYSIEHIYCTAKNAQSYGVDDRIWLKRGSWKKLVSKQKLSKIKYD 170

Query: 702 VIFLSPPWGGPDYAGVDIYDLTK-LKPHDGYFLFNVAKKIAPLVVMFLPKNVNLNQLA 748
            +F SPPWGGP+Y   D+YDL + LKP     +     K++P V+MFLP+N +LNQL+
Sbjct: 171 CVFGSPPWGGPEYLRNDVYDLEQHLKPMGITKMLKSFLKLSPNVIMFLPRNSDLNQLS 228

BLAST of CSPI04G24120 vs. ExPASy TrEMBL
Match: A0A0A0L4V8 (Trimethylguanosine synthase OS=Cucumis sativus OX=3659 GN=Csa_4G639840 PE=4 SV=1)

HSP 1 Score: 1604.7 bits (4154), Expect = 0.0e+00
Identity = 785/788 (99.62%), Postives = 787/788 (99.87%), Query Frame = 0

Query: 1   MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE 60
           MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE
Sbjct: 1   MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE 60

Query: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFL 120
           KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFL
Sbjct: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFL 120

Query: 121 DKEVEFPKASSREEIVANNTFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDI 180
           DKEVEFPKASSREEIVAN+TFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDI
Sbjct: 121 DKEVEFPKASSREEIVANSTFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDI 180

Query: 181 SRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPR 240
           SRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPR
Sbjct: 181 SRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPR 240

Query: 241 LRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGIGLPEVAEPCFLRMEPDC 300
           LRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGI LPEVAEPCFLRMEPDC
Sbjct: 241 LRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGIVLPEVAEPCFLRMEPDC 300

Query: 301 NENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNES 360
           NENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNES
Sbjct: 301 NENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNES 360

Query: 361 TAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFI 420
           TAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFI
Sbjct: 361 TAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFI 420

Query: 421 DTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFS 480
           DTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFS
Sbjct: 421 DTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFS 480

Query: 481 HHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQ 540
           HHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQ
Sbjct: 481 HHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQ 540

Query: 541 NLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKM 600
           NLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKM
Sbjct: 541 NLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKM 600

Query: 601 DKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA 660
           DKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA
Sbjct: 601 DKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA 660

Query: 661 QHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGY 720
           QHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGY
Sbjct: 661 QHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGY 720

Query: 721 FLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNG 780
           FLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNG
Sbjct: 721 FLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNG 780

Query: 781 SINKDNVT 789
           +INKDNVT
Sbjct: 781 NINKDNVT 788

BLAST of CSPI04G24120 vs. ExPASy TrEMBL
Match: A0A1S3BU54 (Trimethylguanosine synthase OS=Cucumis melo OX=3656 GN=LOC103493545 PE=4 SV=1)

HSP 1 Score: 1438.7 bits (3723), Expect = 0.0e+00
Identity = 714/789 (90.49%), Postives = 733/789 (92.90%), Query Frame = 0

Query: 1   MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE 60
           MGSCNEESEDEPGVS IRALGSLFKLTEVFLWD+ETE+ARR+ESRLALDADDANN K VE
Sbjct: 1   MGSCNEESEDEPGVSAIRALGSLFKLTEVFLWDDETEIARRLESRLALDADDANNEKPVE 60

Query: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKE-KRIGITMVKRKANVKHSRIQQGF 120
           KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKE KRIGITM KRKA VKHSRIQQGF
Sbjct: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEPKRIGITMGKRKATVKHSRIQQGF 120

Query: 121 LDKEVEFPKASSREEIVANNTFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGD 180
           LDKEVEFPKAS REEI+ANNTFNDDA GSLC YSMVNQSETSDRDVVLDTNE HVIFDGD
Sbjct: 121 LDKEVEFPKASMREEIIANNTFNDDAIGSLCCYSMVNQSETSDRDVVLDTNETHVIFDGD 180

Query: 181 ISRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSP 240
           ISRNSSGVISGA+EEQFCDV CDIVLNNGGDHELSSDDAVLGDH KVR SSIG DK +S 
Sbjct: 181 ISRNSSGVISGAIEEQFCDVTCDIVLNNGGDHELSSDDAVLGDHIKVRSSSIGLDKDHSS 240

Query: 241 RLRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGIGLPEVAEPCFLRMEPD 300
           RL TTGLDVGHGKQEEVEP MESEGSSTTFQDTEVQ  D DSGIGLPEVAEPC   M  D
Sbjct: 241 RLCTTGLDVGHGKQEEVEPSMESEGSSTTFQDTEVQNIDVDSGIGLPEVAEPC--HMGAD 300

Query: 301 CNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNE 360
            NENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKS ESTWNPP GLEHFASSDANFTPNE
Sbjct: 301 YNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSQESTWNPPSGLEHFASSDANFTPNE 360

Query: 361 STAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAF 420
           S AEV +MDVLEDVKSEDIC VLGDT+CMNLLGD+VHCQPPDALLEGSSSL EG ESSAF
Sbjct: 361 SIAEVPKMDVLEDVKSEDICGVLGDTKCMNLLGDNVHCQPPDALLEGSSSLFEGSESSAF 420

Query: 421 IDTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMF 480
           ++TS+NC+KDEPQ WLMSCRNTRENIG SCEGHAKQSC ENCTNGSQ I A+ ASEQ  F
Sbjct: 421 VNTSVNCNKDEPQAWLMSCRNTRENIGRSCEGHAKQSCCENCTNGSQLIVAHDASEQKTF 480

Query: 481 SHHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTV 540
           SHHKPSNMHSP ID +TIDDDEGTAGLTTS VS MLQQ DHIDGDMHF NGPIICTLGT 
Sbjct: 481 SHHKPSNMHSPGIDYVTIDDDEGTAGLTTSGVSLMLQQEDHIDGDMHFGNGPIICTLGTD 540

Query: 541 QNLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIK 600
           QNLSVR RK+KMKRTRRR QLSDRNE FRSFAITEEYPTSITKYWCQRYQLFSRFDDGIK
Sbjct: 541 QNLSVRYRKKKMKRTRRRAQLSDRNEEFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIK 600

Query: 601 MDKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRY 660
           MDKEGWFSVTPEPIARHHASRCGSNMIID FTGVGGNAIQFSQRAKHVIAIDIDPTKIRY
Sbjct: 601 MDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRY 660

Query: 661 AQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDG 720
           AQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYA VDIYDLTKLKPHDG
Sbjct: 661 AQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLTKLKPHDG 720

Query: 721 YFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSN 780
           YFLFNVAKKIAPL+VMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAY SN
Sbjct: 721 YFLFNVAKKIAPLIVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSN 780

Query: 781 GSINKDNVT 789
            S+NK+NVT
Sbjct: 781 DSMNKNNVT 787

BLAST of CSPI04G24120 vs. ExPASy TrEMBL
Match: A0A1S4DZC6 (Trimethylguanosine synthase OS=Cucumis melo OX=3656 GN=LOC103493545 PE=4 SV=1)

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 639/708 (90.25%), Postives = 655/708 (92.51%), Query Frame = 0

Query: 82  MNALGLPLSFHTNKE-KRIGITMVKRKANVKHSRIQQGFLDKEVEFPKASSREEIVANNT 141
           MNALGLPLSFHTNKE KRIGITM KRKA VKHSRIQQGFLDKEVEFPKAS REEI+ANNT
Sbjct: 1   MNALGLPLSFHTNKEPKRIGITMGKRKATVKHSRIQQGFLDKEVEFPKASMREEIIANNT 60

Query: 142 FNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDISRNSSGVISGAVEEQFCDVM 201
           FNDDA GSLC YSMVNQSETSDRDVVLDTNE HVIFDGDISRNSSGVISGA+EEQFCDV 
Sbjct: 61  FNDDAIGSLCCYSMVNQSETSDRDVVLDTNETHVIFDGDISRNSSGVISGAIEEQFCDVT 120

Query: 202 CDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPRLRTTGLDVGHGKQEEVEPPM 261
           CDIVLNNGGDHELSSDDAVLGDH KVR SSIG DK +S RL TTGLDVGHGKQEEVEP M
Sbjct: 121 CDIVLNNGGDHELSSDDAVLGDHIKVRSSSIGLDKDHSSRLCTTGLDVGHGKQEEVEPSM 180

Query: 262 ESEGSSTTFQDTEVQKSDTDSGIGLPEVAEPCFLRMEPDCNENDQVVGCIHESGDWMVYW 321
           ESEGSSTTFQDTEVQ  D DSGIGLPEVAEPC   M  D NENDQVVGCIHESGDWMVYW
Sbjct: 181 ESEGSSTTFQDTEVQNIDVDSGIGLPEVAEPC--HMGADYNENDQVVGCIHESGDWMVYW 240

Query: 322 DSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICR 381
           DSFYMRNYFYNIKS ESTWNPP GLEHFASSDANFTPNES AEV +MDVLEDVKSEDIC 
Sbjct: 241 DSFYMRNYFYNIKSQESTWNPPSGLEHFASSDANFTPNESIAEVPKMDVLEDVKSEDICG 300

Query: 382 VLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRN 441
           VLGDT+CMNLLGD+VHCQPPDALLEGSSSL EG ESSAF++TS+NC+KDEPQ WLMSCRN
Sbjct: 301 VLGDTKCMNLLGDNVHCQPPDALLEGSSSLFEGSESSAFVNTSVNCNKDEPQAWLMSCRN 360

Query: 442 TRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDD 501
           TRENIG SCEGHAKQSC ENCTNGSQ I A+ ASEQ  FSHHKPSNMHSP ID +TIDDD
Sbjct: 361 TRENIGRSCEGHAKQSCCENCTNGSQLIVAHDASEQKTFSHHKPSNMHSPGIDYVTIDDD 420

Query: 502 EGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQL 561
           EGTAGLTTS VS MLQQ DHIDGDMHF NGPIICTLGT QNLSVR RK+KMKRTRRR QL
Sbjct: 421 EGTAGLTTSGVSLMLQQEDHIDGDMHFGNGPIICTLGTDQNLSVRYRKKKMKRTRRRAQL 480

Query: 562 SDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASR 621
           SDRNE FRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASR
Sbjct: 481 SDRNEEFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASR 540

Query: 622 CGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDF 681
           CGSNMIID FTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDF
Sbjct: 541 CGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDF 600

Query: 682 FRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKN 741
           FRLAPHLKADVIFLSPPWGGPDYA VDIYDLTKLKPHDGYFLFNVAKKIAPL+VMFLPKN
Sbjct: 601 FRLAPHLKADVIFLSPPWGGPDYARVDIYDLTKLKPHDGYFLFNVAKKIAPLIVMFLPKN 660

Query: 742 VNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNGSINKDNVT 789
           VNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAY SN S+NK+NVT
Sbjct: 661 VNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSNDSMNKNNVT 706

BLAST of CSPI04G24120 vs. ExPASy TrEMBL
Match: A0A5D3D9M2 (Trimethylguanosine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003340 PE=4 SV=1)

HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 618/686 (90.09%), Postives = 634/686 (92.42%), Query Frame = 0

Query: 103 MVKRKANVKHSRIQQGFLDKEVEFPKASSREEIVANNTFNDDATGSLCSYSMVNQSETSD 162
           M KRKA VKHSRIQQGFLDKEVEFPKAS REEI+ANNTFNDDA GSLC YSMVNQSETSD
Sbjct: 1   MGKRKATVKHSRIQQGFLDKEVEFPKASMREEIIANNTFNDDAIGSLCCYSMVNQSETSD 60

Query: 163 RDVVLDTNEIHVIFDGDISRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGD 222
           RDVVLDTNE HVIFDGDISRNSSGVISGA+EEQFCDV CDIVLNNGGDHELSSDDAVLGD
Sbjct: 61  RDVVLDTNETHVIFDGDISRNSSGVISGAIEEQFCDVTCDIVLNNGGDHELSSDDAVLGD 120

Query: 223 HTKVRLSSIGFDKGYSPRLRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSG 282
           H KVR SSIG DK +S RL TTGLDVGHGKQEEVEP MESEGSSTTFQDTEVQ  D DSG
Sbjct: 121 HIKVRSSSIGLDKDHSSRLCTTGLDVGHGKQEEVEPSMESEGSSTTFQDTEVQNIDVDSG 180

Query: 283 IGLPEVAEPCFLRMEPDCNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPP 342
           IGLPEVAEPC   M  D NENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKS ESTWNPP
Sbjct: 181 IGLPEVAEPC--HMGADYNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSQESTWNPP 240

Query: 343 LGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDA 402
            GLEHFASSDANFTPNES AEV +MDVLEDVKSEDIC VLGDT+CMNLLGD+VHCQPPDA
Sbjct: 241 SGLEHFASSDANFTPNESIAEVPKMDVLEDVKSEDICGVLGDTKCMNLLGDNVHCQPPDA 300

Query: 403 LLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCT 462
           LLEGSSSL EG ESSAF++TS+NC+KDEPQ WLMSCRNTRENIG SCEGHAKQSC ENCT
Sbjct: 301 LLEGSSSLFEGSESSAFVNTSVNCNKDEPQAWLMSCRNTRENIGRSCEGHAKQSCCENCT 360

Query: 463 NGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHID 522
           NGSQ I A+ ASEQ  FSHHKPSNMHSP ID +TIDDDEGTAGLTTS VS MLQQ DHID
Sbjct: 361 NGSQLIVAHDASEQKTFSHHKPSNMHSPGIDYVTIDDDEGTAGLTTSGVSLMLQQEDHID 420

Query: 523 GDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITK 582
           GDMHF NGPIICTLGT QNLSVR RK+KMKRTRRR QLSDRNE FRSFAITEEYPTSITK
Sbjct: 421 GDMHFGNGPIICTLGTDQNLSVRYRKKKMKRTRRRAQLSDRNEEFRSFAITEEYPTSITK 480

Query: 583 YWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQ 642
           YWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGSNMIID FTGVGGNAIQFSQ
Sbjct: 481 YWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQ 540

Query: 643 RAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPD 702
           RAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPD
Sbjct: 541 RAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPD 600

Query: 703 YAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVE 762
           YA VDIYDLTKLKPHDGYFLFNVAKKIAPL+VMFLPKNVNLNQLAELSLSSDPPWSLEVE
Sbjct: 601 YARVDIYDLTKLKPHDGYFLFNVAKKIAPLIVMFLPKNVNLNQLAELSLSSDPPWSLEVE 660

Query: 763 KNFLNGKLKAITAYLSNGSINKDNVT 789
           KNFLNGKLKAITAY SN S+NK+NVT
Sbjct: 661 KNFLNGKLKAITAYFSNDSMNKNNVT 684

BLAST of CSPI04G24120 vs. ExPASy TrEMBL
Match: A0A1S4E023 (Trimethylguanosine synthase OS=Cucumis melo OX=3656 GN=LOC103493545 PE=4 SV=1)

HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 618/686 (90.09%), Postives = 634/686 (92.42%), Query Frame = 0

Query: 103 MVKRKANVKHSRIQQGFLDKEVEFPKASSREEIVANNTFNDDATGSLCSYSMVNQSETSD 162
           M KRKA VKHSRIQQGFLDKEVEFPKAS REEI+ANNTFNDDA GSLC YSMVNQSETSD
Sbjct: 1   MGKRKATVKHSRIQQGFLDKEVEFPKASMREEIIANNTFNDDAIGSLCCYSMVNQSETSD 60

Query: 163 RDVVLDTNEIHVIFDGDISRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGD 222
           RDVVLDTNE HVIFDGDISRNSSGVISGA+EEQFCDV CDIVLNNGGDHELSSDDAVLGD
Sbjct: 61  RDVVLDTNETHVIFDGDISRNSSGVISGAIEEQFCDVTCDIVLNNGGDHELSSDDAVLGD 120

Query: 223 HTKVRLSSIGFDKGYSPRLRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSG 282
           H KVR SSIG DK +S RL TTGLDVGHGKQEEVEP MESEGSSTTFQDTEVQ  D DSG
Sbjct: 121 HIKVRSSSIGLDKDHSSRLCTTGLDVGHGKQEEVEPSMESEGSSTTFQDTEVQNIDVDSG 180

Query: 283 IGLPEVAEPCFLRMEPDCNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPP 342
           IGLPEVAEPC   M  D NENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKS ESTWNPP
Sbjct: 181 IGLPEVAEPC--HMGADYNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSQESTWNPP 240

Query: 343 LGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDA 402
            GLEHFASSDANFTPNES AEV +MDVLEDVKSEDIC VLGDT+CMNLLGD+VHCQPPDA
Sbjct: 241 SGLEHFASSDANFTPNESIAEVPKMDVLEDVKSEDICGVLGDTKCMNLLGDNVHCQPPDA 300

Query: 403 LLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCT 462
           LLEGSSSL EG ESSAF++TS+NC+KDEPQ WLMSCRNTRENIG SCEGHAKQSC ENCT
Sbjct: 301 LLEGSSSLFEGSESSAFVNTSVNCNKDEPQAWLMSCRNTRENIGRSCEGHAKQSCCENCT 360

Query: 463 NGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHID 522
           NGSQ I A+ ASEQ  FSHHKPSNMHSP ID +TIDDDEGTAGLTTS VS MLQQ DHID
Sbjct: 361 NGSQLIVAHDASEQKTFSHHKPSNMHSPGIDYVTIDDDEGTAGLTTSGVSLMLQQEDHID 420

Query: 523 GDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITK 582
           GDMHF NGPIICTLGT QNLSVR RK+KMKRTRRR QLSDRNE FRSFAITEEYPTSITK
Sbjct: 421 GDMHFGNGPIICTLGTDQNLSVRYRKKKMKRTRRRAQLSDRNEEFRSFAITEEYPTSITK 480

Query: 583 YWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQ 642
           YWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGSNMIID FTGVGGNAIQFSQ
Sbjct: 481 YWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQ 540

Query: 643 RAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPD 702
           RAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPD
Sbjct: 541 RAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPD 600

Query: 703 YAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVE 762
           YA VDIYDLTKLKPHDGYFLFNVAKKIAPL+VMFLPKNVNLNQLAELSLSSDPPWSLEVE
Sbjct: 601 YARVDIYDLTKLKPHDGYFLFNVAKKIAPLIVMFLPKNVNLNQLAELSLSSDPPWSLEVE 660

Query: 763 KNFLNGKLKAITAYLSNGSINKDNVT 789
           KNFLNGKLKAITAY SN S+NK+NVT
Sbjct: 661 KNFLNGKLKAITAYFSNDSMNKNNVT 684

BLAST of CSPI04G24120 vs. NCBI nr
Match: XP_031740708.1 (uncharacterized protein LOC101215034 isoform X2 [Cucumis sativus] >KGN55196.1 hypothetical protein Csa_012563 [Cucumis sativus])

HSP 1 Score: 1604.7 bits (4154), Expect = 0.0e+00
Identity = 785/788 (99.62%), Postives = 787/788 (99.87%), Query Frame = 0

Query: 1   MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE 60
           MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE
Sbjct: 1   MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE 60

Query: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFL 120
           KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFL
Sbjct: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFL 120

Query: 121 DKEVEFPKASSREEIVANNTFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDI 180
           DKEVEFPKASSREEIVAN+TFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDI
Sbjct: 121 DKEVEFPKASSREEIVANSTFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDI 180

Query: 181 SRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPR 240
           SRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPR
Sbjct: 181 SRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPR 240

Query: 241 LRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGIGLPEVAEPCFLRMEPDC 300
           LRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGI LPEVAEPCFLRMEPDC
Sbjct: 241 LRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGIVLPEVAEPCFLRMEPDC 300

Query: 301 NENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNES 360
           NENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNES
Sbjct: 301 NENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNES 360

Query: 361 TAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFI 420
           TAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFI
Sbjct: 361 TAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFI 420

Query: 421 DTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFS 480
           DTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFS
Sbjct: 421 DTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFS 480

Query: 481 HHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQ 540
           HHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQ
Sbjct: 481 HHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQ 540

Query: 541 NLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKM 600
           NLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKM
Sbjct: 541 NLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKM 600

Query: 601 DKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA 660
           DKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA
Sbjct: 601 DKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYA 660

Query: 661 QHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGY 720
           QHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGY
Sbjct: 661 QHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGY 720

Query: 721 FLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNG 780
           FLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNG
Sbjct: 721 FLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNG 780

Query: 781 SINKDNVT 789
           +INKDNVT
Sbjct: 781 NINKDNVT 788

BLAST of CSPI04G24120 vs. NCBI nr
Match: XP_031740706.1 (uncharacterized protein LOC101215034 isoform X1 [Cucumis sativus] >XP_031740707.1 uncharacterized protein LOC101215034 isoform X1 [Cucumis sativus])

HSP 1 Score: 1600.1 bits (4142), Expect = 0.0e+00
Identity = 785/789 (99.49%), Postives = 787/789 (99.75%), Query Frame = 0

Query: 1   MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE 60
           MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE
Sbjct: 1   MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE 60

Query: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKE-KRIGITMVKRKANVKHSRIQQGF 120
           KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKE KRIGITMVKRKANVKHSRIQQGF
Sbjct: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMVKRKANVKHSRIQQGF 120

Query: 121 LDKEVEFPKASSREEIVANNTFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGD 180
           LDKEVEFPKASSREEIVAN+TFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGD
Sbjct: 121 LDKEVEFPKASSREEIVANSTFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGD 180

Query: 181 ISRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSP 240
           ISRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSP
Sbjct: 181 ISRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSP 240

Query: 241 RLRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGIGLPEVAEPCFLRMEPD 300
           RLRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGI LPEVAEPCFLRMEPD
Sbjct: 241 RLRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGIVLPEVAEPCFLRMEPD 300

Query: 301 CNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNE 360
           CNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNE
Sbjct: 301 CNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNE 360

Query: 361 STAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAF 420
           STAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAF
Sbjct: 361 STAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAF 420

Query: 421 IDTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMF 480
           IDTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMF
Sbjct: 421 IDTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMF 480

Query: 481 SHHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTV 540
           SHHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTV
Sbjct: 481 SHHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTV 540

Query: 541 QNLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIK 600
           QNLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIK
Sbjct: 541 QNLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIK 600

Query: 601 MDKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRY 660
           MDKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRY
Sbjct: 601 MDKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRY 660

Query: 661 AQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDG 720
           AQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDG
Sbjct: 661 AQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDG 720

Query: 721 YFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSN 780
           YFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSN
Sbjct: 721 YFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSN 780

Query: 781 GSINKDNVT 789
           G+INKDNVT
Sbjct: 781 GNINKDNVT 789

BLAST of CSPI04G24120 vs. NCBI nr
Match: XP_031740710.1 (uncharacterized protein LOC101215034 isoform X4 [Cucumis sativus])

HSP 1 Score: 1446.8 bits (3744), Expect = 0.0e+00
Identity = 704/707 (99.58%), Postives = 706/707 (99.86%), Query Frame = 0

Query: 82  MNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFLDKEVEFPKASSREEIVANNTF 141
           MNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFLDKEVEFPKASSREEIVAN+TF
Sbjct: 1   MNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFLDKEVEFPKASSREEIVANSTF 60

Query: 142 NDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDISRNSSGVISGAVEEQFCDVMC 201
           NDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDISRNSSGVISGAVEEQFCDVMC
Sbjct: 61  NDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDISRNSSGVISGAVEEQFCDVMC 120

Query: 202 DIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPRLRTTGLDVGHGKQEEVEPPME 261
           DIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPRLRTTGLDVGHGKQEEVEPPME
Sbjct: 121 DIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPRLRTTGLDVGHGKQEEVEPPME 180

Query: 262 SEGSSTTFQDTEVQKSDTDSGIGLPEVAEPCFLRMEPDCNENDQVVGCIHESGDWMVYWD 321
           SEGSSTTFQDTEVQKSDTDSGI LPEVAEPCFLRMEPDCNENDQVVGCIHESGDWMVYWD
Sbjct: 181 SEGSSTTFQDTEVQKSDTDSGIVLPEVAEPCFLRMEPDCNENDQVVGCIHESGDWMVYWD 240

Query: 322 SFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICRV 381
           SFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICRV
Sbjct: 241 SFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICRV 300

Query: 382 LGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRNT 441
           LGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRNT
Sbjct: 301 LGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRNT 360

Query: 442 RENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDDE 501
           RENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDDE
Sbjct: 361 RENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDDE 420

Query: 502 GTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQLS 561
           GTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQLS
Sbjct: 421 GTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQLS 480

Query: 562 DRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRC 621
           DRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRC
Sbjct: 481 DRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRC 540

Query: 622 GSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFF 681
           GSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFF
Sbjct: 541 GSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFF 600

Query: 682 RLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNV 741
           RLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNV
Sbjct: 601 RLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNV 660

Query: 742 NLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNGSINKDNVT 789
           NLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNG+INKDNVT
Sbjct: 661 NLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNGNINKDNVT 707

BLAST of CSPI04G24120 vs. NCBI nr
Match: XP_031740709.1 (uncharacterized protein LOC101215034 isoform X3 [Cucumis sativus])

HSP 1 Score: 1442.2 bits (3732), Expect = 0.0e+00
Identity = 704/708 (99.44%), Postives = 706/708 (99.72%), Query Frame = 0

Query: 82  MNALGLPLSFHTNKE-KRIGITMVKRKANVKHSRIQQGFLDKEVEFPKASSREEIVANNT 141
           MNALGLPLSFHTNKE KRIGITMVKRKANVKHSRIQQGFLDKEVEFPKASSREEIVAN+T
Sbjct: 1   MNALGLPLSFHTNKEQKRIGITMVKRKANVKHSRIQQGFLDKEVEFPKASSREEIVANST 60

Query: 142 FNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDISRNSSGVISGAVEEQFCDVM 201
           FNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDISRNSSGVISGAVEEQFCDVM
Sbjct: 61  FNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDISRNSSGVISGAVEEQFCDVM 120

Query: 202 CDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPRLRTTGLDVGHGKQEEVEPPM 261
           CDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPRLRTTGLDVGHGKQEEVEPPM
Sbjct: 121 CDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSPRLRTTGLDVGHGKQEEVEPPM 180

Query: 262 ESEGSSTTFQDTEVQKSDTDSGIGLPEVAEPCFLRMEPDCNENDQVVGCIHESGDWMVYW 321
           ESEGSSTTFQDTEVQKSDTDSGI LPEVAEPCFLRMEPDCNENDQVVGCIHESGDWMVYW
Sbjct: 181 ESEGSSTTFQDTEVQKSDTDSGIVLPEVAEPCFLRMEPDCNENDQVVGCIHESGDWMVYW 240

Query: 322 DSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICR 381
           DSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICR
Sbjct: 241 DSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICR 300

Query: 382 VLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRN 441
           VLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRN
Sbjct: 301 VLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRN 360

Query: 442 TRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDD 501
           TRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDD
Sbjct: 361 TRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDD 420

Query: 502 EGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQL 561
           EGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQL
Sbjct: 421 EGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQL 480

Query: 562 SDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASR 621
           SDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASR
Sbjct: 481 SDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASR 540

Query: 622 CGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDF 681
           CGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDF
Sbjct: 541 CGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDF 600

Query: 682 FRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKN 741
           FRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKN
Sbjct: 601 FRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKN 660

Query: 742 VNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNGSINKDNVT 789
           VNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNG+INKDNVT
Sbjct: 661 VNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSNGNINKDNVT 708

BLAST of CSPI04G24120 vs. NCBI nr
Match: XP_008452558.1 (PREDICTED: uncharacterized protein LOC103493545 isoform X1 [Cucumis melo] >XP_016901331.1 PREDICTED: uncharacterized protein LOC103493545 isoform X1 [Cucumis melo] >XP_016901332.1 PREDICTED: uncharacterized protein LOC103493545 isoform X1 [Cucumis melo] >XP_016901333.1 PREDICTED: uncharacterized protein LOC103493545 isoform X1 [Cucumis melo])

HSP 1 Score: 1438.7 bits (3723), Expect = 0.0e+00
Identity = 714/789 (90.49%), Postives = 733/789 (92.90%), Query Frame = 0

Query: 1   MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE 60
           MGSCNEESEDEPGVS IRALGSLFKLTEVFLWD+ETE+ARR+ESRLALDADDANN K VE
Sbjct: 1   MGSCNEESEDEPGVSAIRALGSLFKLTEVFLWDDETEIARRLESRLALDADDANNEKPVE 60

Query: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKE-KRIGITMVKRKANVKHSRIQQGF 120
           KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKE KRIGITM KRKA VKHSRIQQGF
Sbjct: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEPKRIGITMGKRKATVKHSRIQQGF 120

Query: 121 LDKEVEFPKASSREEIVANNTFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGD 180
           LDKEVEFPKAS REEI+ANNTFNDDA GSLC YSMVNQSETSDRDVVLDTNE HVIFDGD
Sbjct: 121 LDKEVEFPKASMREEIIANNTFNDDAIGSLCCYSMVNQSETSDRDVVLDTNETHVIFDGD 180

Query: 181 ISRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGDHTKVRLSSIGFDKGYSP 240
           ISRNSSGVISGA+EEQFCDV CDIVLNNGGDHELSSDDAVLGDH KVR SSIG DK +S 
Sbjct: 181 ISRNSSGVISGAIEEQFCDVTCDIVLNNGGDHELSSDDAVLGDHIKVRSSSIGLDKDHSS 240

Query: 241 RLRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGIGLPEVAEPCFLRMEPD 300
           RL TTGLDVGHGKQEEVEP MESEGSSTTFQDTEVQ  D DSGIGLPEVAEPC   M  D
Sbjct: 241 RLCTTGLDVGHGKQEEVEPSMESEGSSTTFQDTEVQNIDVDSGIGLPEVAEPC--HMGAD 300

Query: 301 CNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNE 360
            NENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKS ESTWNPP GLEHFASSDANFTPNE
Sbjct: 301 YNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSQESTWNPPSGLEHFASSDANFTPNE 360

Query: 361 STAEVCEMDVLEDVKSEDICRVLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAF 420
           S AEV +MDVLEDVKSEDIC VLGDT+CMNLLGD+VHCQPPDALLEGSSSL EG ESSAF
Sbjct: 361 SIAEVPKMDVLEDVKSEDICGVLGDTKCMNLLGDNVHCQPPDALLEGSSSLFEGSESSAF 420

Query: 421 IDTSINCSKDEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMF 480
           ++TS+NC+KDEPQ WLMSCRNTRENIG SCEGHAKQSC ENCTNGSQ I A+ ASEQ  F
Sbjct: 421 VNTSVNCNKDEPQAWLMSCRNTRENIGRSCEGHAKQSCCENCTNGSQLIVAHDASEQKTF 480

Query: 481 SHHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTV 540
           SHHKPSNMHSP ID +TIDDDEGTAGLTTS VS MLQQ DHIDGDMHF NGPIICTLGT 
Sbjct: 481 SHHKPSNMHSPGIDYVTIDDDEGTAGLTTSGVSLMLQQEDHIDGDMHFGNGPIICTLGTD 540

Query: 541 QNLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIK 600
           QNLSVR RK+KMKRTRRR QLSDRNE FRSFAITEEYPTSITKYWCQRYQLFSRFDDGIK
Sbjct: 541 QNLSVRYRKKKMKRTRRRAQLSDRNEEFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIK 600

Query: 601 MDKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRY 660
           MDKEGWFSVTPEPIARHHASRCGSNMIID FTGVGGNAIQFSQRAKHVIAIDIDPTKIRY
Sbjct: 601 MDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRY 660

Query: 661 AQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDLTKLKPHDG 720
           AQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYA VDIYDLTKLKPHDG
Sbjct: 661 AQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLTKLKPHDG 720

Query: 721 YFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYLSN 780
           YFLFNVAKKIAPL+VMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAY SN
Sbjct: 721 YFLFNVAKKIAPLIVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSN 780

Query: 781 GSINKDNVT 789
            S+NK+NVT
Sbjct: 781 DSMNKNNVT 787

BLAST of CSPI04G24120 vs. TAIR 10
Match: AT1G45231.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 344.4 bits (882), Expect = 2.5e-94
Identity = 207/517 (40.04%), Postives = 265/517 (51.26%), Query Frame = 0

Query: 287 EVAEPCFLRMEPDC-------NENDQVV--GCI-----------------HESGDWMVYW 346
           EV  PCF   E DC        EN +VV   C                  H+S  W VYW
Sbjct: 117 EVESPCF---EEDCVQVIEVEEENHEVVVGSCFLGNGDGDSVLASETIDSHDSSVWKVYW 176

Query: 347 DSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPNESTAEVCEMDVLEDVKSEDICR 406
           DSFY R+YFYN K+ ES W PPLG+EH A SD +   +E   E    D+   V       
Sbjct: 177 DSFYGRSYFYNFKTQESKWEPPLGMEHLAYSDESHNLSELVIEKHHDDLSGTV------- 236

Query: 407 VLGDTECMNLLGDSVHCQPPDALLEGSSSLIEGIESSAFIDTSINCSKDEPQEWLMSCRN 466
                     LGD V     D                                       
Sbjct: 237 ----------LGDDVPFDKAD--------------------------------------- 296

Query: 467 TRENIGCSCEGHAKQSCGENCTNGSQFIAANGASEQMMFSHHKPSNMHSPEIDCITIDDD 526
              ++G  C+   +    E   +              +   ++ +++ +  +D IT   +
Sbjct: 297 ---DLGGVCQSQLEAEATEEVNS--------------LIDTYQETSIGNQSLD-ITSLGE 356

Query: 527 EGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICTLGTVQNLSVRNRKRKMKRTRRRGQL 586
           EGT     S                                 SVR  K++ +R+R + +L
Sbjct: 357 EGTGAYVVS---------------------------------SVRKAKKESRRSRAKKKL 416

Query: 587 SDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASR 646
            +   G     + EEY   + KYWCQRY LFSRFD+GIKMD+EGWFSVTPE IA+HHA+R
Sbjct: 417 LNSYTGTEMKGVPEEYSPILGKYWCQRYLLFSRFDEGIKMDEEGWFSVTPELIAKHHATR 476

Query: 647 CGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDF 706
           C   ++ID FTGVGGNAIQF+ R+ +VIAID+DP K+  A+HNAAIYGV D+IDF+KGDF
Sbjct: 477 CNEGIVIDCFTGVGGNAIQFASRSHYVIAIDLDPKKLDLAKHNAAIYGVADKIDFVKGDF 523

Query: 707 FRLAPHLKADVIFLSPPWGGPDYAGVDIYDL-TKLKPHDGYFLFNVAKKIAPLVVMFLPK 766
           F LA +LKA  +FLSPPWGGPDY     YD+ T L+P DG  LF  A  IA  ++MFLP+
Sbjct: 537 FDLAHNLKAGTVFLSPPWGGPDYLKASTYDMKTMLRPRDGDALFKAAMNIASTIIMFLPR 523

Query: 767 NVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAITAY 777
           NV++NQLAEL+LS+ PPWSLEVEKN+LNGKLKA+TAY
Sbjct: 597 NVDINQLAELALSTSPPWSLEVEKNYLNGKLKAVTAY 523

BLAST of CSPI04G24120 vs. TAIR 10
Match: AT1G30550.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 4.0e-68
Identity = 119/213 (55.87%), Postives = 156/213 (73.24%), Query Frame = 0

Query: 572 ITEEYPTS--ITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGSNMIIDG 631
           I +E+ T+  I++YW QRY LFS++D GI+MD+EGW+SVTPE IA   A RC   ++ID 
Sbjct: 9   IEKEHGTNPKISRYWIQRYDLFSKYDQGIEMDEEGWYSVTPEEIAIKQAERCRGKVVIDC 68

Query: 632 FTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKA 691
           F+GVGGN IQF++    VIAIDIDP KI  A +NA +YGV ++IDF+ GDF +LAP LK 
Sbjct: 69  FSGVGGNTIQFAKVCSSVIAIDIDPMKIALAMNNAKVYGVANRIDFVTGDFMQLAPSLKG 128

Query: 692 DVIFLSPPWGGPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNVNLNQLAEL 751
           DV+FLSPPWGGP Y+ V+ Y L  L P DGY LF  A  I P ++MFLPKN++L QL EL
Sbjct: 129 DVLFLSPPWGGPTYSKVESYKLDMLLPRDGYSLFQTALSITPNIIMFLPKNIDLAQLEEL 188

Query: 752 SLSSDPPWSLEVEKNFLNGKLKAITAYLSNGSI 783
           +  S PP +LE+E+N + G++KAITAY S+  +
Sbjct: 189 ACLSSPPLTLEIEENSIGGEIKAITAYFSSNVV 221

BLAST of CSPI04G24120 vs. TAIR 10
Match: AT1G30550.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 256.1 bits (653), Expect = 8.9e-68
Identity = 113/203 (55.67%), Postives = 152/203 (74.88%), Query Frame = 0

Query: 580 ITKYWCQRYQLFSRFDDGIKMDKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQ 639
           ITKYW QRY LFSR+D GI+MD+EGW+SVTPE IA   A R    ++ID F+GVGGN IQ
Sbjct: 253 ITKYWIQRYDLFSRYDQGIEMDEEGWYSVTPEEIAIKQAQRYRGKVVIDCFSGVGGNTIQ 312

Query: 640 FSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWG 699
           F++    V+AIDIDP K+  A +NA +YGV +++DF+ GDF +LAP LK DV+FLSPPWG
Sbjct: 313 FAKVCSSVVAIDIDPVKVELAMNNAMVYGVANRVDFVIGDFIQLAPSLKGDVVFLSPPWG 372

Query: 700 GPDYAGVDIYDLTKLKPHDGYFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSL 759
           GP Y   + Y+L  L+P DGY LF +A+ I P ++MFLP+NV+L Q+ EL+  S PP +L
Sbjct: 373 GPMYRDFESYNLDMLQPRDGYSLFQIAQSITPNIIMFLPRNVDLAQVEELAWLSSPPLNL 432

Query: 760 EVEKNFLNGKLKAITAYLSNGSI 783
           E+E+NF+ G++KA+TAY S  ++
Sbjct: 433 EIEENFVGGRMKAVTAYFSCNAV 455

BLAST of CSPI04G24120 vs. TAIR 10
Match: AT3G21300.1 (RNA methyltransferase family protein )

HSP 1 Score: 47.4 bits (111), Expect = 6.3e-05
Identity = 25/80 (31.25%), Postives = 41/80 (51.25%), Query Frame = 0

Query: 622 GSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFLKGDFF 681
           GS +++D F G G   +  ++RAKHV   ++ P  I  A  NA I G+E+   F++GD  
Sbjct: 399 GSEVVLDLFCGTGTIGLTLARRAKHVYGYEVVPQAITDAHKNAQINGIEN-ATFIQGDLN 458

Query: 682 RLAPHL-----KADVIFLSP 697
           ++         K D++   P
Sbjct: 459 KIGEDFGNNFPKPDIVISDP 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P851072.2e-6345.85Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1[more]
Q96RS02.1e-6153.92Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3[more]
Q923W14.6e-6146.48Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2[more]
Q098146.5e-3939.47Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484... [more]
Q120523.3e-2741.57Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Match NameE-valueIdentityDescription
A0A0A0L4V80.0e+0099.62Trimethylguanosine synthase OS=Cucumis sativus OX=3659 GN=Csa_4G639840 PE=4 SV=1[more]
A0A1S3BU540.0e+0090.49Trimethylguanosine synthase OS=Cucumis melo OX=3656 GN=LOC103493545 PE=4 SV=1[more]
A0A1S4DZC60.0e+0090.25Trimethylguanosine synthase OS=Cucumis melo OX=3656 GN=LOC103493545 PE=4 SV=1[more]
A0A5D3D9M20.0e+0090.09Trimethylguanosine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S4E0230.0e+0090.09Trimethylguanosine synthase OS=Cucumis melo OX=3656 GN=LOC103493545 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_031740708.10.0e+0099.62uncharacterized protein LOC101215034 isoform X2 [Cucumis sativus] >KGN55196.1 hy... [more]
XP_031740706.10.0e+0099.49uncharacterized protein LOC101215034 isoform X1 [Cucumis sativus] >XP_031740707.... [more]
XP_031740710.10.0e+0099.58uncharacterized protein LOC101215034 isoform X4 [Cucumis sativus][more]
XP_031740709.10.0e+0099.44uncharacterized protein LOC101215034 isoform X3 [Cucumis sativus][more]
XP_008452558.10.0e+0090.49PREDICTED: uncharacterized protein LOC103493545 isoform X1 [Cucumis melo] >XP_01... [more]
Match NameE-valueIdentityDescription
AT1G45231.22.5e-9440.04S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G30550.24.0e-6855.87S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G30550.18.9e-6855.67S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G21300.16.3e-0531.25RNA methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 573..784
e-value: 3.0E-80
score: 270.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 244..281
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 260..279
NoneNo IPR availablePANTHERPTHR14741S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATEDcoord: 24..784
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 626..723
e-value: 3.93394E-9
score: 52.8175
IPR019012RNA cap guanine-N2 methyltransferasePFAMPF09445Methyltransf_15coord: 624..778
e-value: 1.4E-39
score: 135.3
IPR001202WW domainPROSITEPS01159WW_DOMAIN_1coord: 316..342
IPR001202WW domainPROSITEPS50020WW_DOMAIN_2coord: 316..344
score: 9.8715
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 572..739

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G24120.1CSPI04G24120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009452 7-methylguanosine RNA capping
biological_process GO:0001510 RNA methylation
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0005515 protein binding