CSPI04G23700 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G23700
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionEnt-kaurene oxidase
LocationChr4: 21720053 .. 21723269 (+)
RNA-Seq ExpressionCSPI04G23700
SyntenyCSPI04G23700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCATAGAGTTTGTTTTTGTTTGGCGTATTCGCTGGCTTTTCTATGCATGTCTTGCACAAATGCCAACGCAGTTCTTTCTTTCGCCATTCAATTCCTCAATTTTATAAGAATCTCCGTCTTCTTCTTCTCATTCTGCCACTTCCTCTGTTTTCACTGACTTCAGAGTTCCAAAATGGCGGTTGTTACTGATCCCCTTGCTTCCATGCAACTTCTCGCCAATACCATTCCAGCTCCGCCGTATGCGGCAGCCGCTGTTCTGGGCGGCGTCTCTTTGGTACTTTCCGTGTTTTTTGTTGCTGATTGTAGAAAGAAACGTCGGAATTTTCTCCCTCCGGTGCCGGGTAACCATCGTGCTCTTCTCTCTCTCTCTTTTTCAATCATGATAAATTCTCATTTTGATTATTGGGTTTTTAGCGAAAATTGGTGATTTGATGAATTTGGGAATTTCGGTCTGGTTTTTGTAGCGGTTCCGGGGGTTCCGGTGTTGGGGAATTTGCTGCAGTTGAAGGAGAAGAAGCCTCACAAGACATTTGCTCGATGGGCGGAGACGTACGGTGCGGTTTATTCAATAAGAACCGGCGCTTCCACTGTGATTGTTCTCAACACCACAGAGGTTGCCAAAGAGGTAAAAATAAAATAAAACAAAATCCCGGCTGTTTTTCCGGCGAAGTTTTGGGCTCTGTTTTATGATTCTAACCGTGCTTTGTTTTGGTGTTGATTGTTCTTAAGGCTATGGTGACGAGGTATGGTTCGATTTCGAGTCGAAAGCTAAGCAAAGCTCTTACAATCTTGACTGCTGATAAATGCATGGTTGCTATGAGTGATTACAATGAGTTTCACAAGATGGTGAAGAGATATATATTGGCTAATGTGTTGGGGGCTAATGCTCAGGTTGTTTGTTTCTTCTTCTTCTTTACTATTTATTGTTCATTTAATTTGGGTTAAATCTTATAGTTTGATTTGTTTCGTTTCGTTTCTCAGAAGAAACACAGACAAAGGAGAGACGCCATGATTGAAAATATTTCTAGGGAGTTGTTTGCTCATGTGAAGGAGTTTCCATTAGACACTGTTAATTTTCGGAAGATCTTCGAAGCCGAACTCTTTCGACTCGCGCTTAAAGAAGTGAGTTGATTATGTTCTTCTGTTGAATGCAAAACTTGGAATGAATTGGATGTATGTGTGTGTGTGGTAGATAGAATGGAGGATGGATAAATATAGCTAACTTTCATGATTTCGTTAACGTAGACGCTTGGGAAGGATATTGAGTCGATATATGTGGATGGGCTTGGTACGACATTGCCGAGGGAAGATCTGTTTAGGATTTTAGTGATTGATCCAATGGAGGGTGCGATTGAGGTTGATTGGAGGGATTTCTTCCCATATTTGAGATGGATTCCAAACAAGAGGGTGGAGAATAAAATAAGGAATATGGATTTTCGAAGGCGAATGACAATGAAGAAACTAATGGAGGAGCCAAAGAAACGAATTGCAGCAGGGGAGGTAGTTCTTCCTTCCTTCTCTTGAATTGAAATGAGGCTATGTATCTACTTTGTTTTGACTTGGCTTGAATCTATAACAGGAGACGTATTGCTATGCCGATTTCTTGTTATCTGAAGCGAAAACACTTACCGAGGATCAAATATCCATGTTGCTTTGGGAGACAATCATTGAAACCTCTGACACAACTTTAGTTGTAACAGAATGGGCGATGTATGAACTTTCGAAAGATCCAAGACGACAGGCAATTTCCGAACGAAAATACCATTTTTTTCCTCTTTTGCTAGTCTTTGCTATCCTTAAGATCATCAAAACCAATCTATATTCTCTTAAAAATTCTGACATTGCAGGACTATCTATATCAGCAGATACAGAGTGTTTGTGGATCTGCAACGCTTACTGAAGAAAACTTGTCACAGCTACCATATCTGACAGCTATTTTTCATGAAACTCTTCGGAAGCATAGTCCGGTCCCAGTCGTCCCATTACGGTATGCGCATGAAGACACCCAGTTAGGCGGGTACTTCGTTCCTGCAGGGAGTGAGGTGAGCTATCCTACAACCCTGAAACCTAAGTGCAATTTGATACAACAATGTCTAATGATGTTTCGTTTTCAGATATACAACAATGTCTAATGATGTTTCGTTTTCAGATCGCCGTGAACATTTACGCATGCAACATGGACAAGGATCATTGGGAAAGCCCTGAGGAATGGAAGCCAGAGAGGTTTCTGGATGACAAGTATGATCCAATGGATTTGCACAAAACCATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTCAAGGCAATGCTGATAGCTTGCACAACCATTGGAAGGATGGTTCAAGAGTTTGAGTGGAAGCTGAGGGAAGGAGAAGAGGAGAAGGTCGACACTCTTGGCCTCACCGCTCGCAAGCTTCAGCCACTGCACGTGGTCATAAAACCAAGAAACAACTGATGAAACTTCCCCCTCAGTCCTAGGAACCTAGAATCTAGCTCTTTGGACACCCTTAGCCTCTTGTTGAAGAGAAACTAGTGTGTTTCAGAGCAGTCTAGAACTACAACTATCGTTTAATAATAGGTGCGCACAGATCGAATTTTAGCCCATAATAAGTTTGAATTTTAGCTCAAAAACAACTGAGGTGATGTGTGTTGCATATGATATGGTAATTCAATGTGTTTTCCTACAAACAAGAGATTGTGATATCATAAATGAAACCTTAACAGTGATTAATACAGTAACAAACCAGATTTTCCACGTCTCCATAGAGAATTTTACAAACTTTTTATCTATATCCACAAAGATATCGATACTACTACGTCGATCGCGTATGTAGCATGAGATGGCACGCCTTTAGGAAAATCTCAGTTGCTTAGAAATTCAAGTTCACACTCTTGCCATATATTATGATGCTCTGCTCTGATGATTCTTGGATCAACTGGGTTTGAAGAACTTCAACTTAAGTCCACTGTTTACCAAACTCCATATTCCTCCAATTGAGAAAGCCAAGCTAAAAGCAATGCCTAGCCAACCCAATGTCCAGTGGAAATACCAATTGAAACTGAACTTTGTAGGCTTTTTTATGAGAACCCACATGAAGCAAGGGTAAGCAAATGTGACTGGAAGAGTGAGTCCTCCAAGCAGACCAGCTAAACTGGAGAGGAAAGGAAGTGCTACTCCTATAAAGAAGTTGACAAATCCATAGAAAA

mRNA sequence

ATCATAGAGTTTGTTTTTGTTTGGCGTATTCGCTGGCTTTTCTATGCATGTCTTGCACAAATGCCAACGCAGTTCTTTCTTTCGCCATTCAATTCCTCAATTTTATAAGAATCTCCGTCTTCTTCTTCTCATTCTGCCACTTCCTCTGTTTTCACTGACTTCAGAGTTCCAAAATGGCGGTTGTTACTGATCCCCTTGCTTCCATGCAACTTCTCGCCAATACCATTCCAGCTCCGCCGTATGCGGCAGCCGCTGTTCTGGGCGGCGTCTCTTTGGTACTTTCCGTGTTTTTTGTTGCTGATTGTAGAAAGAAACGTCGGAATTTTCTCCCTCCGGTGCCGGCGGTTCCGGGGGTTCCGGTGTTGGGGAATTTGCTGCAGTTGAAGGAGAAGAAGCCTCACAAGACATTTGCTCGATGGGCGGAGACGTACGGTGCGGTTTATTCAATAAGAACCGGCGCTTCCACTGTGATTGTTCTCAACACCACAGAGGTTGCCAAAGAGGCTATGGTGACGAGGTATGGTTCGATTTCGAGTCGAAAGCTAAGCAAAGCTCTTACAATCTTGACTGCTGATAAATGCATGGTTGCTATGAGTGATTACAATGAGTTTCACAAGATGGTGAAGAGATATATATTGGCTAATGTGTTGGGGGCTAATGCTCAGAAGAAACACAGACAAAGGAGAGACGCCATGATTGAAAATATTTCTAGGGAGTTGTTTGCTCATGTGAAGGAGTTTCCATTAGACACTGTTAATTTTCGGAAGATCTTCGAAGCCGAACTCTTTCGACTCGCGCTTAAAGAAACGCTTGGGAAGGATATTGAGTCGATATATGTGGATGGGCTTGGTACGACATTGCCGAGGGAAGATCTGTTTAGGATTTTAGTGATTGATCCAATGGAGGGTGCGATTGAGGTTGATTGGAGGGATTTCTTCCCATATTTGAGATGGATTCCAAACAAGAGGGTGGAGAATAAAATAAGGAATATGGATTTTCGAAGGCGAATGACAATGAAGAAACTAATGGAGGAGCCAAAGAAACGAATTGCAGCAGGGGAGGAGACGTATTGCTATGCCGATTTCTTGTTATCTGAAGCGAAAACACTTACCGAGGATCAAATATCCATGTTGCTTTGGGAGACAATCATTGAAACCTCTGACACAACTTTAGTTGTAACAGAATGGGCGATGTATGAACTTTCGAAAGATCCAAGACGACAGGACTATCTATATCAGCAGATACAGAGTGTTTGTGGATCTGCAACGCTTACTGAAGAAAACTTGTCACAGCTACCATATCTGACAGCTATTTTTCATGAAACTCTTCGGAAGCATAGTCCGGTCCCAGTCGTCCCATTACGGTATGCGCATGAAGACACCCAGTTAGGCGGGTACTTCGTTCCTGCAGGGAGTGAGATCGCCGTGAACATTTACGCATGCAACATGGACAAGGATCATTGGGAAAGCCCTGAGGAATGGAAGCCAGAGAGGTTTCTGGATGACAAGTATGATCCAATGGATTTGCACAAAACCATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTCAAGGCAATGCTGATAGCTTGCACAACCATTGGAAGGATGGTTCAAGAGTTTGAGTGGAAGCTGAGGGAAGGAGAAGAGGAGAAGGTCGACACTCTTGGCCTCACCGCTCGCAAGCTTCAGCCACTGCACGTGGTCATAAAACCAAGAAACAACTGATGAAACTTCCCCCTCAGTCCTAGGAACCTAGAATCTAGCTCTTTGGACACCCTTAGCCTCTTGTTGAAGAGAAACTAGTGTGTTTCAGAGCAGTCTAGAACTACAACTATCGTTTAATAATAGGTGCGCACAGATCGAATTTTAGCCCATAATAAGTTTGAATTTTAGCTCAAAAACAACTGAGGTGATGTGTGTTGCATATGATATGGTAATTCAATGTGTTTTCCTACAAACAAGAGATTGTGATATCATAAATGAAACCTTAACAGTGATTAATACAGTAACAAACCAGATTTTCCACGTCTCCATAGAGAATTTTACAAACTTTTTATCTATATCCACAAAGATATCGATACTACTACGTCGATCGCGTATGTAGCATGAGATGGCACGCCTTTAGGAAAATCTCAGTTGCTTAGAAATTCAAGTTCACACTCTTGCCATATATTATGATGCTCTGCTCTGATGATTCTTGGATCAACTGGGTTTGAAGAACTTCAACTTAAGTCCACTGTTTACCAAACTCCATATTCCTCCAATTGAGAAAGCCAAGCTAAAAGCAATGCCTAGCCAACCCAATGTCCAGTGGAAATACCAATTGAAACTGAACTTTGTAGGCTTTTTTATGAGAACCCACATGAAGCAAGGGTAAGCAAATGTGACTGGAAGAGTGAGTCCTCCAAGCAGACCAGCTAAACTGGAGAGGAAAGGAAGTGCTACTCCTATAAAGAAGTTGACAAATCCATAGAAAA

Coding sequence (CDS)

ATGGCGGTTGTTACTGATCCCCTTGCTTCCATGCAACTTCTCGCCAATACCATTCCAGCTCCGCCGTATGCGGCAGCCGCTGTTCTGGGCGGCGTCTCTTTGGTACTTTCCGTGTTTTTTGTTGCTGATTGTAGAAAGAAACGTCGGAATTTTCTCCCTCCGGTGCCGGCGGTTCCGGGGGTTCCGGTGTTGGGGAATTTGCTGCAGTTGAAGGAGAAGAAGCCTCACAAGACATTTGCTCGATGGGCGGAGACGTACGGTGCGGTTTATTCAATAAGAACCGGCGCTTCCACTGTGATTGTTCTCAACACCACAGAGGTTGCCAAAGAGGCTATGGTGACGAGGTATGGTTCGATTTCGAGTCGAAAGCTAAGCAAAGCTCTTACAATCTTGACTGCTGATAAATGCATGGTTGCTATGAGTGATTACAATGAGTTTCACAAGATGGTGAAGAGATATATATTGGCTAATGTGTTGGGGGCTAATGCTCAGAAGAAACACAGACAAAGGAGAGACGCCATGATTGAAAATATTTCTAGGGAGTTGTTTGCTCATGTGAAGGAGTTTCCATTAGACACTGTTAATTTTCGGAAGATCTTCGAAGCCGAACTCTTTCGACTCGCGCTTAAAGAAACGCTTGGGAAGGATATTGAGTCGATATATGTGGATGGGCTTGGTACGACATTGCCGAGGGAAGATCTGTTTAGGATTTTAGTGATTGATCCAATGGAGGGTGCGATTGAGGTTGATTGGAGGGATTTCTTCCCATATTTGAGATGGATTCCAAACAAGAGGGTGGAGAATAAAATAAGGAATATGGATTTTCGAAGGCGAATGACAATGAAGAAACTAATGGAGGAGCCAAAGAAACGAATTGCAGCAGGGGAGGAGACGTATTGCTATGCCGATTTCTTGTTATCTGAAGCGAAAACACTTACCGAGGATCAAATATCCATGTTGCTTTGGGAGACAATCATTGAAACCTCTGACACAACTTTAGTTGTAACAGAATGGGCGATGTATGAACTTTCGAAAGATCCAAGACGACAGGACTATCTATATCAGCAGATACAGAGTGTTTGTGGATCTGCAACGCTTACTGAAGAAAACTTGTCACAGCTACCATATCTGACAGCTATTTTTCATGAAACTCTTCGGAAGCATAGTCCGGTCCCAGTCGTCCCATTACGGTATGCGCATGAAGACACCCAGTTAGGCGGGTACTTCGTTCCTGCAGGGAGTGAGATCGCCGTGAACATTTACGCATGCAACATGGACAAGGATCATTGGGAAAGCCCTGAGGAATGGAAGCCAGAGAGGTTTCTGGATGACAAGTATGATCCAATGGATTTGCACAAAACCATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTCAAGGCAATGCTGATAGCTTGCACAACCATTGGAAGGATGGTTCAAGAGTTTGAGTGGAAGCTGAGGGAAGGAGAAGAGGAGAAGGTCGACACTCTTGGCCTCACCGCTCGCAAGCTTCAGCCACTGCACGTGGTCATAAAACCAAGAAACAACTGA

Protein sequence

MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPGVPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISRELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSVCGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN*
Homology
[Close]
BLAST of CSPI04G23700 vs. ExPASy Swiss-Prot
Match: A0A0G2RKY1 (Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 9.8e-193
Identity = 330/507 (65.09%), Postives = 401/507 (79.09%), Query Frame = 0 

Query: 17  TIPAPPYAAA-----AVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPGVPVLGNLLQLK 76
           ++ A P AAA       +GG++L     +V D RKK  +F PP P VPG+PV+GNLLQLK
Sbjct: 6   SLQAVPAAAAIGGPVVAIGGITLFFIREYVKDQRKKSSSF-PPPPKVPGLPVIGNLLQLK 65

Query: 77  EKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSISSRKLSKALTIL 136
           EKKPHKTF +W+E YG +YSIRTGA+T+IVLNT +VAKEAM+T+Y SIS+RKLSKALTIL
Sbjct: 66  EKKPHKTFTKWSEKYGPIYSIRTGANTMIVLNTNDVAKEAMITKYSSISTRKLSKALTIL 125

Query: 137 TADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISRELFAHVKEFPL 196
           T+DK +VAMSDYNEF+K  KR++L + LG  AQK+HR  R+ MI NI  +  AH K +P 
Sbjct: 126 TSDKSIVAMSDYNEFYKAAKRHLLTSTLGPTAQKRHRVHRNLMINNICDQFLAHAKMYPS 185

Query: 197 DTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVIDPMEGAIEVDW 256
           + VNFRKIF++ELF L++K+ +G+D+ESIYV+ L TTL R+++F+ILV+DPMEGAI+VDW
Sbjct: 186 EAVNFRKIFQSELFGLSMKQAIGEDVESIYVEDLDTTLSRQEMFKILVVDPMEGAIDVDW 245

Query: 257 RDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYCYADFLLSE-AK 316
           RDFFPYL+WIPN+  ENKI+ M F R   MK L+E+ KKRIA+G+   CY D LLSE A 
Sbjct: 246 RDFFPYLKWIPNQHFENKIQQMHFHREAVMKALIEQQKKRIASGKAINCYLDHLLSEAAD 305

Query: 317 TLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSVCGSATLTEEN 376
           TL+E QI MLLWE IIE SDTTLV TEWAMYELSKDP+RQ+YL  +IQ+ CG   L EE 
Sbjct: 306 TLSEQQILMLLWEAIIEASDTTLVTTEWAMYELSKDPKRQNYLLSEIQNACGFDQLNEEK 365

Query: 377 LSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNIYACNMDKDHW 436
           L +LPYL AIF ETLRKHSPVPVVPLRY HE+TQLGGY +P GSEIA+NIY CNMDK+ W
Sbjct: 366 LCRLPYLAAIFQETLRKHSPVPVVPLRYVHEETQLGGYTIPEGSEIAINIYGCNMDKNVW 425

Query: 437 ESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGRMVQEFEWKLR 496
           +SPEEW+PERF+  K D  +LHKTMAFGGGKRVCAGAL+AM I+C  IGR+VQE EW+L 
Sbjct: 426 DSPEEWRPERFVFGKDDTTELHKTMAFGGGKRVCAGALQAMTISCIAIGRLVQELEWRLG 485

Query: 497 EGEEEKVDTLGLTARKLQPLHVVIKPR 518
           +GEE  VDTLGLT  KL PL  +IKPR
Sbjct: 486 DGEEANVDTLGLTTHKLHPLQTIIKPR 511

[Close]
BLAST of CSPI04G23700 vs. ExPASy Swiss-Prot
Match: Q93ZB2 (Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV=2)

HSP 1 Score: 632.9 bits (1631), Expect = 3.3e-180
Identity = 309/493 (62.68%), Postives = 383/493 (77.69%), Query Frame = 0 

Query: 33  SLVLSVFFVADCRKKRRNF-----LPPVPAVPGVPVLGNLLQLKEKKPHKTFARWAETYG 92
           S +   FF       R+N      LP VP VPG PV+GNLLQLKEKKPHKTF RW+E YG
Sbjct: 17  SFIFIFFFKKLLSFSRKNMSEVSTLPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYG 76

Query: 93  AVYSIRTGASTVIVLNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFH 152
            +YSI+ G+S++IVLN+TE AKEAMVTR+ SIS+RKLS ALT+LT DK MVA SDY++FH
Sbjct: 77  PIYSIKMGSSSLIVLNSTETAKEAMVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFH 136

Query: 153 KMVKRYILANVLGANAQKKHRQRRDAMIENISRELFAHVKEFPLDTVNFRKIFEAELFRL 212
           K+VKR +L  +LGANAQK+ R  RDA+IEN+S +L AH ++ P + VNFR IFE ELF +
Sbjct: 137 KLVKRCLLNGLLGANAQKRKRHYRDALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGV 196

Query: 213 ALKETLGKDIESIYVDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKRVE 272
           ALK+  GKD+ESIYV  LG TL ++++F++LV D MEGAI+VDWRDFFPYL+WIPNK  E
Sbjct: 197 ALKQAFGKDVESIYVKELGVTLSKDEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFE 256

Query: 273 NKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYCYADFLLSEAKTLTEDQISMLLWETIIE 332
            +I+    RR   M  L+++  K+  +  +  CY +FL+SEAKTLT++QI++L+WETIIE
Sbjct: 257 ARIQQKHKRRLAVMNALIQDRLKQNGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIE 316

Query: 333 TSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSVCGSATLTEENLSQLPYLTAIFHETLRK 392
           T+DTTLV TEWA+YEL+K P  QD L ++IQ+VCG     EE LSQ+PYL  +FHETLRK
Sbjct: 317 TADTTLVTTEWAIYELAKHPSVQDRLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRK 376

Query: 393 HSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDD-KY 452
           +SP P+VP+RYAHEDTQ+GGY VPAGSEIA+NIY CNMDK  WE PE+W PERFLDD KY
Sbjct: 377 YSPAPLVPIRYAHEDTQIGGYHVPAGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKY 436

Query: 453 DPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKVDTLGLTARK 512
           +  DLHKTMAFG GKRVCAGAL+A L+A   IGR+VQEFEWKLR+GEEE VDT GLT++K
Sbjct: 437 ETSDLHKTMAFGAGKRVCAGALQASLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQK 496

Query: 513 LQPLHVVIKPRNN 520
           L PL  +I PR +
Sbjct: 497 LYPLMAIINPRRS 509

[Close]
BLAST of CSPI04G23700 vs. ExPASy Swiss-Prot
Match: Q5Z5R4 (Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 3.6e-163
Identity = 279/500 (55.80%), Postives = 372/500 (74.40%), Query Frame = 0 

Query: 18  IPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPGVPVLGNLLQLKEKKPHK 77
           +P    AAAA +GG     ++   A     R+    P PAVPG+P++GNL QLKEKKPH+
Sbjct: 5   VPGGAGAAAAAVGGFVAAAALAERAGVIAPRKRPNAP-PAVPGLPIIGNLHQLKEKKPHQ 64

Query: 78  TFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCM 137
           TFA+WAE YG +Y+IRTGAS+V+VLN+TEVAKEAMV ++ SIS+RKLSKALT+LT DK M
Sbjct: 65  TFAKWAEIYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLTRDKSM 124

Query: 138 VAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISRELFAHVKEFPLDTVNFR 197
           VA SDY +FHKMVKRY+++++LG +AQK+ R  RD MI N+       VK+ P   + FR
Sbjct: 125 VATSDYCDFHKMVKRYVMSSMLGTSAQKQFRDIRDMMIHNMLSTFHKLVKDDPHAPLIFR 184

Query: 198 KIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPY 257
            +F+ ELFRL++ ++LG+D+ S+YVD  G  + +E+++   V D M  AIEVDWRDFFPY
Sbjct: 185 DVFKDELFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPY 244

Query: 258 LRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYCYADFLLSEAKTLTEDQI 317
           L W+PNK  E ++   + RR   M+ L+++ K+RI  GE   CY DFLL+E  TLT++Q+
Sbjct: 245 LSWVPNKSFETRVFTTETRRTAVMRALIKQQKERIVRGEAKTCYLDFLLAE-NTLTDEQL 304

Query: 318 SMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSVCGSATLTEENLSQLPYL 377
            ML+WE +IE +DTTLV TEWAMYEL+K+P +Q+ LYQ+I+ VCG  T+TEE+L +LPYL
Sbjct: 305 MMLVWEALIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDETVTEEHLPRLPYL 364

Query: 378 TAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWK 437
            A+FHETLR+HSPVP++P R+ HEDT+L GY VPAG+E+ +N+Y CNM++  WESPEEW 
Sbjct: 365 NAVFHETLRRHSPVPLIPPRFVHEDTKLAGYDVPAGTEMVINLYGCNMNRKEWESPEEWV 424

Query: 438 PERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKV 497
           PERF   + +  D++KTMAFG G+R CAG+L+A  IAC  + R VQEF W+LREG+EEKV
Sbjct: 425 PERFAGGRLEVADMYKTMAFGAGRRACAGSLQATHIACAAVARFVQEFGWRLREGDEEKV 484

Query: 498 DTLGLTARKLQPLHVVIKPR 518
           DT+ LTA KL PLHV +  R
Sbjct: 485 DTVQLTAYKLHPLHVHLTRR 502

[Close]
BLAST of CSPI04G23700 vs. ExPASy Swiss-Prot
Match: Q5Z5R7 (Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19 PE=2 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 1.3e-160
Identity = 276/493 (55.98%), Postives = 364/493 (73.83%), Query Frame = 0 

Query: 25  AAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPGVPVLGNLLQLKEKKPHKTFARWAE 84
           AAA +GG   + +        K RRN   P PAVPG+P++GNL QLKEKKPH+TF +WAE
Sbjct: 15  AAAAVGG--FIAAATLAVAPPKNRRN---PPPAVPGLPIIGNLHQLKEKKPHQTFTKWAE 74

Query: 85  TYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYN 144
            YG +Y+IRTGAS+V+VLN+TEVAKEAMV ++ SIS+RKLSKALT+L+ DK MVA SD  
Sbjct: 75  IYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLSHDKSMVATSDSG 134

Query: 145 EFHKMVKRYILANVLGANAQKKHRQRRDAMIENISRELFAHVKEFPLDTVNFRKIFEAEL 204
           +FHKM KRYI+ ++LG +AQK+ R  RD +I N+       VK+ P   + FR +F+ EL
Sbjct: 135 DFHKMGKRYIMLSMLGTSAQKQFRDTRDMIINNMLSTFHQLVKDDPHAPLIFRDVFKDEL 194

Query: 205 FRLALKETLGKDIESIYVDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNK 264
           FRL++ ++LG+D+ S+YVD  G  + +E+++   V D M  AIEVDWRDFFPYL W+PNK
Sbjct: 195 FRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPNK 254

Query: 265 RVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYCYADFLLSEAKTLTEDQISMLLWET 324
             E ++   + RR   M+ L+++ K+RI  GE   CY DFLL+E  TLT++Q+ ML+WE 
Sbjct: 255 SFETRVFTTESRRTAVMRALIKQQKERIVRGEARTCYLDFLLAE-NTLTDEQLMMLVWEA 314

Query: 325 IIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSVCGSATLTEENLSQLPYLTAIFHET 384
           +IE +DTTLV TEWAMYEL+K+P +Q+ LYQ+I+ VCG   +TEE+L  LPYL A+F ET
Sbjct: 315 LIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDEAVTEEHLPWLPYLNAVFQET 374

Query: 385 LRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDD 444
           LR+HSPVP++P R+ +EDT L GY VPAG+E+ +N+Y CNM+K  WESPEEW PERF   
Sbjct: 375 LRRHSPVPLIPPRFVNEDTMLAGYDVPAGTEMVINLYGCNMNKKEWESPEEWAPERFAGG 434

Query: 445 KYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKVDTLGLTA 504
           ++   D++KTMAFG G+RVCAG+L+A  IAC  I R VQEF W+LREG+EEKVDT+ LTA
Sbjct: 435 RFKVADMYKTMAFGAGRRVCAGSLQATHIACAAIARFVQEFGWRLREGDEEKVDTVQLTA 494

Query: 505 RKLQPLHVVIKPR 518
            KL PLHV + PR
Sbjct: 495 YKLHPLHVHLTPR 501

[Close]
BLAST of CSPI04G23700 vs. ExPASy Swiss-Prot
Match: Q0DBF4 (Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A8 PE=1 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 6.2e-147
Identity = 253/496 (51.01%), Postives = 354/496 (71.37%), Query Frame = 0 

Query: 24  AAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPGVPVLGNLLQLKEKKPHKTFARWA 83
           A AAV G V+       +      R+N   P PAVPG+P++GNL QLKEKKPH+TFA+W+
Sbjct: 13  AVAAVGGLVAAAALADKLVAAPPPRKNRANPPPAVPGLPIIGNLHQLKEKKPHQTFAKWS 72

Query: 84  ETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDY 143
           ETYG +Y+I+TGAS V+VLN+TEVAKEAM+ ++ SIS+RKL KA+++LT  K MVA+SDY
Sbjct: 73  ETYGPIYTIKTGASPVVVLNSTEVAKEAMIDKFSSISTRKLPKAMSVLTR-KSMVAISDY 132

Query: 144 NEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISRELFAHVKEFPLDTVNFRKIFEAE 203
            ++ KM KR I+  +LG NAQK+ R  R+ MI N+   L   V   P   +NFR ++  E
Sbjct: 133 GDYQKMAKRNIMIGMLGFNAQKQFRGTRERMISNVLSTLHKLVSLDPHSPLNFRDVYINE 192

Query: 204 LFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPN 263
           LF L+L ++LG+D+ S+YV+  G  + ++++F +LV + M  A+E DWRD+FPYL W+PN
Sbjct: 193 LFSLSLIQSLGEDVSSVYVEEFGREISKDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPN 252

Query: 264 KRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYCYADFLLSEAKT--LTEDQISMLL 323
           K  +  +   +FRR   M  L+++ K+RIA GE    Y DFLL   ++  LT+DQ+ +LL
Sbjct: 253 KSFDTIVSTTEFRRDAIMNALIKKQKERIARGEARASYIDFLLEAERSAQLTDDQLMLLL 312

Query: 324 WETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSVCGSATLTEENLSQLPYLTAIF 383
            E+I+  +DT LV TEW MYE++K+P +Q+ LYQ+I+  CG   +TE++L +LPYL A+F
Sbjct: 313 SESILAAADTVLVTTEWTMYEIAKNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVF 372

Query: 384 HETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWKPERF 443
           HETLR HSPVPV+P R+ H+DT L GY + AG+++ +N+YAC+MD+  WESP EW PERF
Sbjct: 373 HETLRLHSPVPVLPPRFVHDDTTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPERF 432

Query: 444 LDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKVDTLG 503
           L + ++  D +KTMAFG G+R CAG+L+AM IAC  + R+VQE EW+LREG+ +K DT+ 
Sbjct: 433 LGEGFEVADRYKTMAFGAGRRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQ 492

Query: 504 LTARKLQPLHVVIKPR 518
            TA KL PLHV +KPR
Sbjct: 493 FTALKLDPLHVHLKPR 507

[Close]
BLAST of CSPI04G23700 vs. ExPASy TrEMBL
Match: J7I3T1 (Ent-kaurene oxidase OS=Cucumis sativus OX=3659 GN=ko PE=2 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 1.6e-299
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0 

Query: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60
           MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG
Sbjct: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
           VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS
Sbjct: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
           SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
           ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI
Sbjct: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300
           DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360

Query: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
           CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI
Sbjct: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
           YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR
Sbjct: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 520
           MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]
BLAST of CSPI04G23700 vs. ExPASy TrEMBL
Match: A0A5D3D9I5 (Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003760 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 4.7e-291
Identity = 502/519 (96.72%), Postives = 513/519 (98.84%), Query Frame = 0 

Query: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60
           MAVVTDPLASMQLLANT+PAPPYAAAAVLGGVSLVLSVF VADCRKKRRNFLPPVPAVPG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
           VP+LGNLLQLKEKKPHKTFARWAETYG VYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
           SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
           ELFAHVKEFP DTVNFRKIFE+ELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300
           DPMEGAIEVDWRDFFPYLRWIPNK VENKIRNMDFRRRMTMK L++EPK+RIAAGEET C
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDP+RQDYLYQQIQSV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
           CGS+TLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
           YACNMDK+HWE+PEEWKPERFLD+KYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 520
           MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]
BLAST of CSPI04G23700 vs. ExPASy TrEMBL
Match: A0A1S3BTZ0 (ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 4.7e-291
Identity = 502/519 (96.72%), Postives = 513/519 (98.84%), Query Frame = 0 

Query: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60
           MAVVTDPLASMQLLANT+PAPPYAAAAVLGGVSLVLSVF VADCRKKRRNFLPPVPAVPG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
           VP+LGNLLQLKEKKPHKTFARWAETYG VYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
           SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
           ELFAHVKEFP DTVNFRKIFE+ELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300
           DPMEGAIEVDWRDFFPYLRWIPNK VENKIRNMDFRRRMTMK L++EPK+RIAAGEET C
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDP+RQDYLYQQIQSV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
           CGS+TLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
           YACNMDK+HWE+PEEWKPERFLD+KYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 520
           MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]
BLAST of CSPI04G23700 vs. ExPASy TrEMBL
Match: A0A6J1IL06 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476578 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 6.9e-258
Identity = 438/517 (84.72%), Postives = 481/517 (93.04%), Query Frame = 0 

Query: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60
           MA+  DPL S+Q L +T+ APPYAAAA+LG +S +L+ F VAD RK RRNFLPPVPA+PG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
           +PVLGNLLQLKEKKPHKTFARWAETYG +YSIRTGASTVIVLNT EVAKEAMVTRY S+S
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
            RKLSKALTILT+DKCMVAMSDYN+FHKMVK+YILANVLGANAQK+HRQRRD+MI+NIS 
Sbjct: 121 GRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180

Query: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
           +LFAHVK+ P ++VNFRKIFE+ELF LALKETLGKDIESIYVDGLGTTLPREDLFR+LVI
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300
           D MEGAIEVDWRDFFPYL+WIPNK VENKIR MD+RRR+TM  L+EE KKRIAAGEET C
Sbjct: 241 DLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360
           YADFLLSEAKTLTE QI+MLLWETIIETSDTTLVVTEWAMYEL+K+P RQDYLYQ IQSV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPERQDYLYQHIQSV 360

Query: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
           CGS  LTE++LS+LPYLTA+FHETLRKHSPVP+VPLRY HED QLGG+FVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
           YACNMDKD WESPEEWKPERFLD+KYDPMDLHKTMAFGGGKRVCAGALKAMLIACT+IGR
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPR 518
           +VQEFEWKL EGEE+KVDTLGLTARKLQPLHV++KPR
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPR 517

[Close]
BLAST of CSPI04G23700 vs. ExPASy TrEMBL
Match: A0A6J1F7C8 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442772 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 5.5e-255
Identity = 434/517 (83.95%), Postives = 479/517 (92.65%), Query Frame = 0 

Query: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60
           M +  DPL S+Q L +T+ AP YAAA++LG +S +L+ F VAD RK RRNFLPPVPA+PG
Sbjct: 1   MVIFNDPLGSLQHLLDTLQAPTYAAASILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
           +PVLGNLLQLKEKKPHKTFARWAETYG +YSIRTGASTVIVLNT EVAKEAMVTRY S+S
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
           SRKLSKALTILT+DKCMVAMSDYN+FHKMVK+YILANVLGANAQK+HRQRRD MI+NIS 
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDTMIDNISS 180

Query: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
           +LFAHVK+ P ++VNFR+IFE+ELFRLALKETLGKDIESIYVDGLG  LPREDLFR+LVI
Sbjct: 181 QLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIYVDGLG-MLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300
           DPMEGAIEVDWRDFFPYL+WIPNK VENKIR MDFRRR+TM  L+E+ KKRIAAGEET C
Sbjct: 241 DPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFRRRVTMMSLIEKQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360
           YADFLLSEAKTLTE QI+MLLWETIIETSDTTLVVTEWAMYEL+K+P+RQDYLYQ IQSV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPKRQDYLYQHIQSV 360

Query: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
           CGS  LTE++LS+LPYLTA+FHETLRKHSPVP+VPLRY HED QLGG+FVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
           YACNMDKD WESPEEWKPERFLD+KYDPMDLHKTMAFGGGKRVCAGALKAMLIACT+IGR
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPR 518
           +VQEFEWKL EGEE+KVDTLGLTARKLQPLH ++KPR
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHAIVKPR 516

[Close]
BLAST of CSPI04G23700 vs. NCBI nr
Match: NP_001267703.1 (ent-kaurene oxidase, chloroplastic-like [Cucumis sativus] >AFH03045.1 ent-kaurene oxidase [Cucumis sativus] >AFQ32799.1 ent-kaurene oxidase [Cucumis sativus] >KGN55152.1 hypothetical protein Csa_012319 [Cucumis sativus])

HSP 1 Score: 1037.7 bits (2682), Expect = 3.3e-299
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0 

Query: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60
           MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG
Sbjct: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
           VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS
Sbjct: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
           SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
           ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI
Sbjct: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300
           DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360

Query: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
           CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI
Sbjct: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
           YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR
Sbjct: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 520
           MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]
BLAST of CSPI04G23700 vs. NCBI nr
Match: XP_008452488.1 (PREDICTED: ent-kaurene oxidase, chloroplastic [Cucumis melo] >KAA0064350.1 ent-kaurene oxidase [Cucumis melo var. makuwa] >TYK20237.1 ent-kaurene oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 1009.6 bits (2609), Expect = 9.8e-291
Identity = 502/519 (96.72%), Postives = 513/519 (98.84%), Query Frame = 0 

Query: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60
           MAVVTDPLASMQLLANT+PAPPYAAAAVLGGVSLVLSVF VADCRKKRRNFLPPVPAVPG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
           VP+LGNLLQLKEKKPHKTFARWAETYG VYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
           SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
           ELFAHVKEFP DTVNFRKIFE+ELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300
           DPMEGAIEVDWRDFFPYLRWIPNK VENKIRNMDFRRRMTMK L++EPK+RIAAGEET C
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDP+RQDYLYQQIQSV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
           CGS+TLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
           YACNMDK+HWE+PEEWKPERFLD+KYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 520
           MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]
BLAST of CSPI04G23700 vs. NCBI nr
Match: XP_038898390.1 (ent-kaurene oxidase [Benincasa hispida])

HSP 1 Score: 965.7 bits (2495), Expect = 1.6e-277
Identity = 477/519 (91.91%), Postives = 498/519 (95.95%), Query Frame = 0 

Query: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60
           MAVVTDPL SMQLLANT+ APPYAAAAVLGGVSLVLSVF VADCRKKRRNFLPPVPA+PG
Sbjct: 1   MAVVTDPLTSMQLLANTLQAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
           VPVLGNLLQLKEKKPH TFARWAE YG VYSIRTGASTVIVLNT EVAKEAMVTRY SIS
Sbjct: 61  VPVLGNLLQLKEKKPHMTFARWAEKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYASIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
           SRKLSKALTILTADKCMVAMSDYN+FHKMVKRYILAN+LGANAQKKHRQRRD MIENISR
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANMLGANAQKKHRQRRDTMIENISR 180

Query: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
           ELFAHVKEFP ++VNFRKIFE+ELFRLALKETLGKDIESIYVDGLGTTLPREDLFR+LVI
Sbjct: 181 ELFAHVKEFPSESVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300
           DPMEGAIEVDWRDFFPYLRWIPNK VENKIR MDF+R+MTM+ L+EE KKRIAAGEET C
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRKMDFQRQMTMRSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYEL+KDP+RQD+LYQ I S 
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDFLYQHILSA 360

Query: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
           CGS+ LTEENLSQLPYLTA+FHETLRKHSPVPVVPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
           YACNMDKDHWESPEEWKPERFLD+KYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR
Sbjct: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 520
           M QEFEWKLREGEE+KVDTLGLTARKLQPLHVV+KPR N
Sbjct: 481 MAQEFEWKLREGEEDKVDTLGLTARKLQPLHVVVKPRIN 519

[Close]
BLAST of CSPI04G23700 vs. NCBI nr
Match: XP_023536271.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 901.0 bits (2327), Expect = 4.9e-258
Identity = 438/517 (84.72%), Postives = 482/517 (93.23%), Query Frame = 0 

Query: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60
           MA+  DPL S+Q L +T+ AP YAAAA+LG +S +L+ FFVAD RK RRNFLPPVPA+PG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPTYAAAAILGVISFLLAGFFVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
           VP+LGNLLQLKEKKPHKTFARWAE YG +YSIRTGASTVIVLNT EVAKEAMVTR+ S+S
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAERYGPIYSIRTGASTVIVLNTAEVAKEAMVTRHASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
           SRKLSKALTILT+DKCMVAMSDYN+FHKMVK+YILANVLGANAQK+HRQRRD+MI+NIS 
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180

Query: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
           +LFAHVK+ P ++VNFRKIFE+ELFRLALKET+GKDIESIYVDGL TTLPREDLFR+LVI
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFRLALKETVGKDIESIYVDGLSTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300
           DPMEGAIEVDWRDFFPYL+WIPNK VENKIR MDFRRR+TM  L+EE KKRIAAGEET C
Sbjct: 241 DPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFRRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360
           YADFLLSEAKTLTE QI+MLLWETIIETSDTTLVVTEWAMYEL+K+P+RQDYLYQ IQSV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPKRQDYLYQHIQSV 360

Query: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
           CGS  LTE++LS+LPYLTA+FHETLRKHSPVP+VPLRY HED QLGG+FVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
           YACNMDKD WESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACT+IGR
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPR 518
           +VQEFEWKL EGEE+KVDTLGLTARKLQPLH ++KPR
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHAIVKPR 517

[Close]
BLAST of CSPI04G23700 vs. NCBI nr
Match: XP_022976063.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 899.4 bits (2323), Expect = 1.4e-257
Identity = 438/517 (84.72%), Postives = 481/517 (93.04%), Query Frame = 0 

Query: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60
           MA+  DPL S+Q L +T+ APPYAAAA+LG +S +L+ F VAD RK RRNFLPPVPA+PG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120
           +PVLGNLLQLKEKKPHKTFARWAETYG +YSIRTGASTVIVLNT EVAKEAMVTRY S+S
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180
            RKLSKALTILT+DKCMVAMSDYN+FHKMVK+YILANVLGANAQK+HRQRRD+MI+NIS 
Sbjct: 121 GRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180

Query: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240
           +LFAHVK+ P ++VNFRKIFE+ELF LALKETLGKDIESIYVDGLGTTLPREDLFR+LVI
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300
           D MEGAIEVDWRDFFPYL+WIPNK VENKIR MD+RRR+TM  L+EE KKRIAAGEET C
Sbjct: 241 DLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360
           YADFLLSEAKTLTE QI+MLLWETIIETSDTTLVVTEWAMYEL+K+P RQDYLYQ IQSV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPERQDYLYQHIQSV 360

Query: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420
           CGS  LTE++LS+LPYLTA+FHETLRKHSPVP+VPLRY HED QLGG+FVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480
           YACNMDKD WESPEEWKPERFLD+KYDPMDLHKTMAFGGGKRVCAGALKAMLIACT+IGR
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPR 518
           +VQEFEWKL EGEE+KVDTLGLTARKLQPLHV++KPR
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPR 517

[Close]
BLAST of CSPI04G23700 vs. TAIR 10
Match: AT5G25900.1 (GA requiring 3 )

HSP 1 Score: 632.9 bits (1631), Expect = 2.3e-181
Identity = 309/493 (62.68%), Postives = 383/493 (77.69%), Query Frame = 0 

Query: 33  SLVLSVFFVADCRKKRRNF-----LPPVPAVPGVPVLGNLLQLKEKKPHKTFARWAETYG 92
           S +   FF       R+N      LP VP VPG PV+GNLLQLKEKKPHKTF RW+E YG
Sbjct: 17  SFIFIFFFKKLLSFSRKNMSEVSTLPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYG 76

Query: 93  AVYSIRTGASTVIVLNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFH 152
            +YSI+ G+S++IVLN+TE AKEAMVTR+ SIS+RKLS ALT+LT DK MVA SDY++FH
Sbjct: 77  PIYSIKMGSSSLIVLNSTETAKEAMVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFH 136

Query: 153 KMVKRYILANVLGANAQKKHRQRRDAMIENISRELFAHVKEFPLDTVNFRKIFEAELFRL 212
           K+VKR +L  +LGANAQK+ R  RDA+IEN+S +L AH ++ P + VNFR IFE ELF +
Sbjct: 137 KLVKRCLLNGLLGANAQKRKRHYRDALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGV 196

Query: 213 ALKETLGKDIESIYVDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKRVE 272
           ALK+  GKD+ESIYV  LG TL ++++F++LV D MEGAI+VDWRDFFPYL+WIPNK  E
Sbjct: 197 ALKQAFGKDVESIYVKELGVTLSKDEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFE 256

Query: 273 NKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYCYADFLLSEAKTLTEDQISMLLWETIIE 332
            +I+    RR   M  L+++  K+  +  +  CY +FL+SEAKTLT++QI++L+WETIIE
Sbjct: 257 ARIQQKHKRRLAVMNALIQDRLKQNGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIE 316

Query: 333 TSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSVCGSATLTEENLSQLPYLTAIFHETLRK 392
           T+DTTLV TEWA+YEL+K P  QD L ++IQ+VCG     EE LSQ+PYL  +FHETLRK
Sbjct: 317 TADTTLVTTEWAIYELAKHPSVQDRLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRK 376

Query: 393 HSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDD-KY 452
           +SP P+VP+RYAHEDTQ+GGY VPAGSEIA+NIY CNMDK  WE PE+W PERFLDD KY
Sbjct: 377 YSPAPLVPIRYAHEDTQIGGYHVPAGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKY 436

Query: 453 DPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKVDTLGLTARK 512
           +  DLHKTMAFG GKRVCAGAL+A L+A   IGR+VQEFEWKLR+GEEE VDT GLT++K
Sbjct: 437 ETSDLHKTMAFGAGKRVCAGALQASLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQK 496

Query: 513 LQPLHVVIKPRNN 520
           L PL  +I PR +
Sbjct: 497 LYPLMAIINPRRS 509

[Close]
BLAST of CSPI04G23700 vs. TAIR 10
Match: AT2G40890.1 (cytochrome P450, family 98, subfamily A, polypeptide 3 )

HSP 1 Score: 179.5 bits (454), Expect = 7.0e-45
Identity = 128/486 (26.34%), Postives = 236/486 (48.56%), Query Frame = 0 

Query: 46  KKRRNFLPPVPAVPGVPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTT 105
           ++ R   PP P+    P++GNL  +K  +  + +  WA++YG + S+  G+   +V+++ 
Sbjct: 21  QRLRYKFPPGPSPK--PIVGNLYDIKPVR-FRCYYEWAQSYGPIISVWIGSILNVVVSSA 80

Query: 106 EVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQK 165
           E+AKE +      ++ R  +++    + +   +  +DY   +  V++     +      +
Sbjct: 81  ELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVCTLELFTPKRLE 140

Query: 166 KHRQRRD----AMIENISRELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIY 225
             R  R+    AM+E++ R+   ++ E     +  RK   A  F    +   GK     +
Sbjct: 141 SLRPIREDEVTAMVESVFRD--CNLPENRAKGLQLRKYLGAVAFNNITRLAFGKR----F 200

Query: 226 VDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTM 285
           ++  G    +   F+ +V + ++    +   +  P+LRW+     E        RR    
Sbjct: 201 MNAEGVVDEQGLEFKAIVSNGLKLGASLSIAEHIPWLRWM-FPADEKAFAEHGARRDRLT 260

Query: 286 KKLMEE---PKKRIAAGEETYCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEW 345
           + +MEE    +++ +  ++ +  A   L +   L+ED I  LLW+ I    DTT +  EW
Sbjct: 261 RAIMEEHTLARQKSSGAKQHFVDALLTLKDQYDLSEDTIIGLLWDMITAGMDTTAITAEW 320

Query: 346 AMYELSKDPRRQDYLYQQIQSVCG-SATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLR 405
           AM E+ K+PR Q  + ++   V G    LTE + S+LPYL  +  E+ R H P P++   
Sbjct: 321 AMAEMIKNPRVQQKVQEEFDRVVGLDRILTEADFSRLPYLQCVVKESFRLHPPTPLMLPH 380

Query: 406 YAHEDTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDDKYDPMDLH--KTM 465
            ++ D ++GGY +P GS + VN++A   D   W++P E++PERFL++  D M  H  + +
Sbjct: 381 RSNADVKIGGYDIPKGSNVHVNVWAVARDPAVWKNPFEFRPERFLEEDVD-MKGHDFRLL 440

Query: 466 AFGGGKRVCAGALKAMLIACTTIGRMVQEFEWKLREG-EEEKVD---TLGLTARKLQPLH 518
            FG G+RVC GA   + +  + +  ++  F W   +G + E++D     GL      P+ 
Sbjct: 441 PFGAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGTKPEEIDMSENPGLVTYMRTPVQ 495

[Close]
BLAST of CSPI04G23700 vs. TAIR 10
Match: AT1G74550.1 (cytochrome P450, family 98, subfamily A, polypeptide 9 )

HSP 1 Score: 164.5 bits (415), Expect = 2.3e-40
Identity = 122/498 (24.50%), Postives = 239/498 (47.99%), Query Frame = 0 

Query: 28  VLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPGVPVLGNLLQLKEKKPHKTFARWAETYG 87
           +L  ++ ++   ++ + R++  N +PP P  P   ++GNL QLK     ++F+ W++TYG
Sbjct: 4   LLISLTTIIIAAYMQNLRRRGSN-IPPGP--PTRFLVGNLHQLKPLWT-QSFSEWSQTYG 63

Query: 88  AVYSIRTGASTVIVLNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFH 147
            + S+  G+   +V++++++AK+ +  +   + +R  +  +T   +D   +  SDY   +
Sbjct: 64  PIISVWLGSQLAVVVSSSDLAKQVLRDKDYQLCNRHRTARMTQNGSD---LIWSDYGAHY 123

Query: 148 KMVKRYILANVLGANAQKKHRQRRDAMIENISRELFAHVKEFPLDTVNFRKIFEAELFRL 207
             +++     +    + +  R  R+  + ++ + +F          V  R   ++    +
Sbjct: 124 VKMRKLCTLELFSLKSIECFRSMREMEVSSMVKSIFNDFMSDDQKPVVLRNYLDSVALNI 183

Query: 208 ALKETLGKDIESIYVDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKRVE 267
             +  +GK  E    DG      RE  FR +V             D+  +L+ + +    
Sbjct: 184 VSRLVIGKTFEP--KDG------RE--FRSIVERETRLPGATKMLDYTVWLKRLSSWFTS 243

Query: 268 NK--IRNMDFRRRMTMKKLMEEPKKRIAAGEETYCYADFL--LSEAKTLTEDQISMLLWE 327
           +K  +++M  +R    + +M+E    +  G +  C+   L  L E   LTE+ +  L+W 
Sbjct: 244 DKAFMKHMARKRNWFKRAVMDE----VYGGRDQKCFVQSLLELKEKDELTEETVMGLVWN 303

Query: 328 TIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSVCGSATL-TEENLSQLPYLTAIFH 387
            +   +DTT +  EWAM E+ + P  ++ +  ++ SV GS  L ++ ++ +LP+L  +  
Sbjct: 304 MLTAGADTTAITIEWAMAEMIRCPTVKEKVQDELDSVVGSGRLMSDADIPKLPFLQCVLK 363

Query: 388 ETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFL 447
           E LR H P P++    A E  Q+GGY VP G+ + VN+ A   D  +W +P+E++PERFL
Sbjct: 364 EALRLHPPTPLMLPHKASESVQVGGYKVPKGATVYVNVQAIARDPANWSNPDEFRPERFL 423

Query: 448 DDKYDPMDL-HKTMAFGGGKRVCAGALKAMLIACTTIGRMVQEFEWKLREGEE--EKVDT 507
            ++ D      + + FG G+RVC  A  ++ +    +G ++  F W      E  +  + 
Sbjct: 424 VEETDVKGQDFRVLPFGSGRRVCPAAQLSLNMMTLALGSLLHCFSWTSSTPREHIDMTEK 480

Query: 508 LGLTARKLQPLHVVIKPR 518
            GL      PL  +   R
Sbjct: 484 PGLVCYMKAPLQALASSR 480

[Close]
BLAST of CSPI04G23700 vs. TAIR 10
Match: AT1G74540.1 (cytochrome P450, family 98, subfamily A, polypeptide 8 )

HSP 1 Score: 161.4 bits (407), Expect = 2.0e-39
Identity = 128/480 (26.67%), Postives = 227/480 (47.29%), Query Frame = 0 

Query: 49  RNFLPPVPAVPGVPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVA 108
           R+ +PP P      +LGNL Q+K    H +F+ W+ETYG + S+  G+   +V++++++A
Sbjct: 24  RSNIPPGPKPKF--LLGNLHQMKPLWTH-SFSEWSETYGPIISVWIGSQLTVVVSSSDLA 83

Query: 109 KEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHR 168
           ++ +  +   +S+R     +T    D   +  SDY+  +  +++     +    + +  R
Sbjct: 84  RQVLRDKDHQLSNRHRIARMTQTGTD---LVWSDYSPHYVKLRKLCTLELFSLKSIENFR 143

Query: 169 QRRD----AMIENISRELFAHV-KEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVD 228
             R+    +M+ +I ++L ++   +     V  RK   A +     +  +GK+  S    
Sbjct: 144 SLREMEARSMVVSILKDLMSNSGDDQERKPVIVRKYLAAVVLNTISRLMIGKEFGSEEGK 203

Query: 229 GLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKRVENK--IRNMDFRRRMTM 288
                + +E L        + G+  +   D   +L+W+ +    +K  + + D R +   
Sbjct: 204 EFKAIVEKEHL--------LSGSGTI--LDHVWWLKWVSSWFFSDKEFLAHKDRRTKWFR 263

Query: 289 KKLMEEPKKRIAAGEETYCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMY 348
             +M E    I      +     +L E K L+E+ +  L+W  +   +DTT VV EWAM 
Sbjct: 264 GAIMVEEDIEI-EDHRGFVRKLLVLKEQKELSEETVGGLVWNMLTAGADTTAVVIEWAMA 323

Query: 349 ELSKDPRRQDYLYQQIQSVCGSATL-TEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAH 408
           E+ K P  Q+   Q++ SV GS  L TE ++  LPYL  +  E LR H   P++    A 
Sbjct: 324 EMIKCPTVQEKAQQELDSVVGSERLMTESDIPILPYLQCVVKEALRLHPSTPLMLPHKAS 383

Query: 409 EDTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDDKYDPMDL-HKTMAFGG 468
           E   +GGY VP G+ + VN+ A   D  +W +P E++PERFL ++ D      + + FG 
Sbjct: 384 ETVWVGGYKVPKGATVYVNVQAIGRDPANWINPYEFRPERFLQEETDVKGRDFRVLPFGS 443

Query: 469 GKRVCAGALKAMLIACTTIGRMVQEFEWKLR-EGEE-EKVDTLGLTARKLQPLHVVIKPR 518
           G+R+C  A  +M +    +G ++  F W     GE  +  +  GL      PL  +  PR
Sbjct: 444 GRRMCPAAQLSMNLMTLVMGNLLHCFSWSSPVPGERIDMSENPGLLCNMRTPLQALALPR 486

[Close]
BLAST of CSPI04G23700 vs. TAIR 10
Match: AT3G26290.1 (cytochrome P450, family 71, subfamily B, polypeptide 26 )

HSP 1 Score: 159.1 bits (401), Expect = 9.8e-39
Identity = 133/505 (26.34%), Postives = 232/505 (45.94%), Query Frame = 0 

Query: 32  VSLVLSVFFVADCRKKRRNF--LPPVPAVPGVPVLGNLLQLKEKKPHKTFARWAETYGAV 91
           +SL+  + F+     KR+N      +P+ PG P++GNL QL E + H++  + ++ YG V
Sbjct: 7   LSLLFFIIFLLLAAFKRKNHGKHRRIPSPPGFPIIGNLHQLGELQ-HQSLWKLSKKYGPV 66

Query: 92  YSIRTGASTVIVLNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFHKM 151
             ++ G    ++L+++E AK+A+        SR        L+ +   ++ S YNE+ K 
Sbjct: 67  MLLKLGKVPTLILSSSETAKQALRDYDLHCCSRPSLAGGRELSYNNLDMSSSPYNEYWKE 126

Query: 152 VKRYILANVLGANAQKKHRQRRDAMIENISRELFAHVKEFPL-DTVNFRKIFEAELFRLA 211
           +++     +  AN  +  +  +D   E + + + +  +   L + VN  K F A    + 
Sbjct: 127 LRKLCSQELFSANKIQSIQPIKD---EEVKKVIDSIAESSSLKNPVNLSKTFLALTTSVV 186

Query: 212 LKETLGKDIESIYVDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNK---- 271
            K   G   E       G+ L   D F  LV D  E        DF PY+ WI +K    
Sbjct: 187 CKAAFGVSFE-------GSVL-NSDRFNKLVRDTFEMLGSFSASDFIPYVGWIIDKFNGL 246

Query: 272 --RVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYCYADFLLSEAK--------TLTE 331
               +   R++D       +++ +  K+    G E     D LL   K         LT 
Sbjct: 247 QGWRKKSFRDLD----AFYEQIFDLHKEEKEVGSED--LVDVLLRLEKEEIVVGNGKLTR 306

Query: 332 DQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQS-VCGSATLTEENLSQ 391
           + I  +L   ++   DT+ +   WAM EL+K+PR    +  +I++ +     ++ ++  +
Sbjct: 307 NHIKAILMNILLGGIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDK 366

Query: 392 LPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNIYACNMDKDHWESP 451
           L YL  +  ET R H P P++  R    + ++ GY +PA + + VN++A   D D W+ P
Sbjct: 367 LEYLKMVIKETWRLHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDP 426

Query: 452 EEWKPERFLDDKYDPMDLH-KTMAFGGGKRVCAGALKAMLIACTTIGRMVQEFEWKLREG 511
           E + PERF D   D    + + ++FG G+R+C G      +    +  M+  F+WKL EG
Sbjct: 427 EMFLPERFNDSNIDAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEG 486

Query: 512 ----EEEKVDTLGLTARKLQPLHVV 514
               + +  +  GLT  K   L +V
Sbjct: 487 MVVEDIDMEEAPGLTVSKKSELVLV 493

The following BLAST results are available for this feature:
BLAST of CSPI04G23700 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A0A0G2RKY19.8e-19365.09Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1[more]
Q93ZB23.3e-18062.68Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV... [more]
Q5Z5R43.6e-16355.80Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 ... [more]
Q5Z5R71.3e-16055.98Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19... [more]
Q0DBF46.2e-14751.01Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 ... [more]
BLAST of CSPI04G23700 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
J7I3T11.6e-299100.00Ent-kaurene oxidase OS=Cucumis sativus OX=3659 GN=ko PE=2 SV=1[more]
A0A5D3D9I54.7e-29196.72Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G... [more]
A0A1S3BTZ04.7e-29196.72ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 ... [more]
A0A6J1IL066.9e-25884.72ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476... [more]
A0A6J1F7C85.5e-25583.95ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
BLAST of CSPI04G23700 vs. NCBI nr
Match NameE-valueIdentityDescription
NP_001267703.13.3e-299100.00ent-kaurene oxidase, chloroplastic-like [Cucumis sativus] >AFH03045.1 ent-kauren... [more]
XP_008452488.19.8e-29196.72PREDICTED: ent-kaurene oxidase, chloroplastic [Cucumis melo] >KAA0064350.1 ent-k... [more]
XP_038898390.11.6e-27791.91ent-kaurene oxidase [Benincasa hispida][more]
XP_023536271.14.9e-25884.72ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022976063.11.4e-25784.72ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima][more]
BLAST of CSPI04G23700 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G25900.12.3e-18162.68GA requiring 3 [more]
AT2G40890.17.0e-4526.34cytochrome P450, family 98, subfamily A, polypeptide 3 [more]
AT1G74550.12.3e-4024.50cytochrome P450, family 98, subfamily A, polypeptide 9 [more]
AT1G74540.12.0e-3926.67cytochrome P450, family 98, subfamily A, polypeptide 8 [more]
AT3G26290.19.8e-3926.34cytochrome P450, family 71, subfamily B, polypeptide 26 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 83..102
score: 32.52
coord: 464..487
score: 23.02
coord: 419..443
score: 35.54
coord: 454..464
score: 51.84
coord: 107..128
score: 21.34
coord: 336..362
score: 25.47
coord: 378..396
score: 29.71
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 45..519
e-value: 4.8E-93
score: 314.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 53..517
IPR001128Cytochrome P450PFAMPF00067p450coord: 57..499
e-value: 1.5E-68
score: 231.6
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 27..489
e-value: 3.7E-13
score: 47.0
IPR044225Ent-kaurene oxidase, chloroplasticPANTHERPTHR47283ENT-KAURENE OXIDASE, CHLOROPLASTICcoord: 23..518
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 457..466

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G23700.1CSPI04G23700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010241 ent-kaurene oxidation to kaurenoic acid
biological_process GO:0009686 gibberellin biosynthetic process
cellular_component GO:0009707 chloroplast outer membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0052615 ent-kaurene oxidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen