CSPI04G23560 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G23560
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin-protein ligase RMA3
Location	Chr4: 21612030 .. 21614830 (+)
RNA-Seq Expression	CSPI04G23560
Synteny	CSPI04G23560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTCCTTTCTATATAATTCCCATTTTCCCCTTCGTTTCTTTTTCTATCTCTTTACATTTGTTCTTCCTCTAATTCTCTCCCTCAACATTTTCTTGATTTGATTTACGTCCGAGATTGTCGTACCATACCTTTCAGTTTCTTATTTAACGGTGAGTGTTTATTTTTTTATTTTTTTTATTTTTCATGTTGATGATTTCTTAAGTTACGTTTCGTTTGATTCTTTGTTTGAGTTTTTGTTATGATTTTGATGGAAATGTGTCAGGGGGAATTGGGGTTTTTGTTTGAATGAATGGGTATTTCTTTGTTTAAGAAAACGAGCCCTGGGTTTTGATCTATGGTTATTGGGCTTTTGGTGATTTTGTTCTGACTTCAATTTGAACTGCTGCCCTTTTCTTATTTCGTTGTGATTCGATCTGGGTTGATTTTTCGGAGAATTTTTATTTTTATGGCGTTTGGTTTGTTTCCCTTTTTTGTCTTTTCGCCTCTAGTTTTTCAACTTTGAAAGTTTCGAGATTTCTTCTAAAGACTTGCTTCCGATCATACACGTTTCTCTCTGTTCGCAATTTGGAGGATAAGTATCGTAAACTACAAACTGAAGGGGTCGATATTTTAAATGTCGATTTGCCGTTTTGATTGTTATTTATTTGGGATTCTTGCATTGTTGCTCTGCAATTGGTTACCATCTTTTGCCCAAAAAGAAATGTTTTTCTTTGTCTCTTTCTTTATAGAGGGTGTTGTGAGCCACGATTGTGGTTTGAGACACCTGGTTTTTTTATGGTCTGAGTATTTTGTATTTTGCATTCATTGTATGAGTTTGTGTAGAAGTTTCTCGATGCAAGTCATTGGTATCTTACATTCAGCATTGATCCTCCCAGGTCTTGTGTCTATTGATCGACCCTTGAATCTTTGATATCTGTGTTTTCTTTTAAGTTTTCAATCCTTTTGGTCTCTTTGCTATATAAATTGGGTTAAGTTGGTAGTGGACAGAACAGAGCTTTAGTTTTGTATACCGTTGTGAAATCCTGTTCCTTAGTTCTTGTTTTCTTTTCTAACACGAGAGCCCTCCAAGGAAACACTGCCAAATTCCAACATCAGAAACAAAATTTTAAAAATTAATCTTTTAAGTTTTCAATACTTTACTTGGATTTTGAAAACATTCCTACAAAATCTATAACAATAGATAACAAAACAAAGAATCTCACTAGTGTAACTAATGTTTTTTTATCTTAATTTTAAAAAACTAAATGATTATCAAATGGGGCCGTTTCTAAATGTTTCAATATTGACTACCAGCTGTTGGTGGTTGAATATTTGATTTTTATTGACTTTATCTAATTAAAGTGCTATTTAGCCTCTCGATTAGCCTCCCTGGTGAAAGTCCGTTGTTTGATTATTCTTCCTTTGTGTGGAAGCTTCTATTCTTCCTTGAAATTTGTTTTGCATTTTTGTAATCCCTTTTTCTTTTTTCTTTTTAGAAAAACAACTGGTATAAGAGGTAATTTTATTGTATTTGCATTATTGCAAATGGGGCATTGTTATTTGTATGTCCAAATTGGTATTTGTCTATAATGTTTTTCAATCGTTTATAGCAGCTGATTTTATTCTTCAAGTTACTTATGTTCCAAGAATTTCATTGTCTTTTTTTTGTTTTTCTTTCTTTTTAAACAGAACGCATACTCTTTCCATGGAACAAAACTACTCTGTACCTGAGGCATACCTAGAGTCTGAACAAGATGTCTCTCTCAAGCAAAACTGGAAATCTATCTCAACTCAGTCAACAATATCAGAAGACGCAAACGGTTGCTTTGATTGCAACATTTGCTTAGACTCAGCAGCTGACCCTGTTGTCACCCTCTGTGGTCACCTCTACTGCTGGCCGTGCATTTACAAATGGCTTCATGTTCAAATCTCCAGCAACGAACCCGAAAACACCCAGAACTGCCCTGTTTGTAAGGCTAGCATTACACCCTCCTCGTTGGTTCCGCTCTATGGGCGTGGCACGTCGAACTCAGATTCCGAATCCAAGAAATCTCATTTGGGTATGGCTGTACCCCGAAGACCACCACCGTCAATGAACACACCATCCCATTCCAATTCATCCTCGGCATTGTATCCTAGCCAGGAGCTTCATTCAAACTATATCCGGTCGCCCTCACACCCAATCTATCACCAACAATATTTCCCCCAGGCCACCTATGGCAACTTTGCCTCGTACTCACCATCTTATCTTGGTAATGCCGTGATAACGAGTCTACTAAACCCAACGATTGGGATGTTTGGAGAAACAGTTTTCACTAGAATATTTGGTAGCGTGGATGGGAACTTATTGCCATACTCGCCATACAACAACTCAATCTCAGGAAATGCCAGTACGAGAATGAGAAGACAGGAAATGCAGCTTGATAAGTCTCTTAATAGAGTTTCCATCTTTCTATTTTGCTGCTTTATTATATGTCTTCTGTTATTTTGAAGTTGGGAATAGATGTGTGGAGAAGCATATGATGATATACATACAGGTAAGCCAGCTAGAATTTTGGTAGATGACTTTACTGTGAGAAGTCGTCTAATATGTACAGAAGGTTAAAGCTAATACCACATATTGTTTTGTAATTCCTTTTGGTGCTCTGTGTGTATTGCATGTTTATAATACTTCTCTACGTTTTCCCACCCCTAGAAAGTCTAATGATTCATCATCTCCAAACAATATCAAGTCTAATCTGTTTTTCCCCTAGACATTGATATAAAAATCATCTTATTATGTGGTTGTACAATTTAAAGCTGAAAATAATTGAGTTGGGTT

mRNA sequence

CTCTCCTTTCTATATAATTCCCATTTTCCCCTTCGTTTCTTTTTCTATCTCTTTACATTTGTTCTTCCTCTAATTCTCTCCCTCAACATTTTCTTGATTTGATTTACGTCCGAGATTGTCGTACCATACCTTTCAGTTTCTTATTTAACGAACGCATACTCTTTCCATGGAACAAAACTACTCTGTACCTGAGGCATACCTAGAGTCTGAACAAGATGTCTCTCTCAAGCAAAACTGGAAATCTATCTCAACTCAGTCAACAATATCAGAAGACGCAAACGGTTGCTTTGATTGCAACATTTGCTTAGACTCAGCAGCTGACCCTGTTGTCACCCTCTGTGGTCACCTCTACTGCTGGCCGTGCATTTACAAATGGCTTCATGTTCAAATCTCCAGCAACGAACCCGAAAACACCCAGAACTGCCCTGTTTGTAAGGCTAGCATTACACCCTCCTCGTTGGTTCCGCTCTATGGGCGTGGCACGTCGAACTCAGATTCCGAATCCAAGAAATCTCATTTGGGTATGGCTGTACCCCGAAGACCACCACCGTCAATGAACACACCATCCCATTCCAATTCATCCTCGGCATTGTATCCTAGCCAGGAGCTTCATTCAAACTATATCCGGTCGCCCTCACACCCAATCTATCACCAACAATATTTCCCCCAGGCCACCTATGGCAACTTTGCCTCGTACTCACCATCTTATCTTGGTAATGCCGTGATAACGAGTCTACTAAACCCAACGATTGGGATGTTTGGAGAAACAGTTTTCACTAGAATATTTGGTAGCGTGGATGGGAACTTATTGCCATACTCGCCATACAACAACTCAATCTCAGGAAATGCCAGTACGAGAATGAGAAGACAGGAAATGCAGCTTGATAAGTCTCTTAATAGAGTTTCCATCTTTCTATTTTGCTGCTTTATTATATGTCTTCTGTTATTTTGAAGTTGGGAATAGATGTGTGGAGAAGCATATGATGATATACATACAGGTAAGCCAGCTAGAATTTTGGTAGATGACTTTACTGTGAGAAGTCGTCTAATATGTACAGAAGGTTAAAGCTAATACCACATATTGTTTTGTAATTCCTTTTGGTGCTCTGTGTGTATTGCATGTTTATAATACTTCTCTACGTTTTCCCACCCCTAGAAAGTCTAATGATTCATCATCTCCAAACAATATCAAGTCTAATCTGTTTTTCCCCTAGACATTGATATAAAAATCATCTTATTATGTGGTTGTACAATTTAAAGCTGAAAATAATTGAGTTGGGTT

Coding sequence (CDS)

ATGGAACAAAACTACTCTGTACCTGAGGCATACCTAGAGTCTGAACAAGATGTCTCTCTCAAGCAAAACTGGAAATCTATCTCAACTCAGTCAACAATATCAGAAGACGCAAACGGTTGCTTTGATTGCAACATTTGCTTAGACTCAGCAGCTGACCCTGTTGTCACCCTCTGTGGTCACCTCTACTGCTGGCCGTGCATTTACAAATGGCTTCATGTTCAAATCTCCAGCAACGAACCCGAAAACACCCAGAACTGCCCTGTTTGTAAGGCTAGCATTACACCCTCCTCGTTGGTTCCGCTCTATGGGCGTGGCACGTCGAACTCAGATTCCGAATCCAAGAAATCTCATTTGGGTATGGCTGTACCCCGAAGACCACCACCGTCAATGAACACACCATCCCATTCCAATTCATCCTCGGCATTGTATCCTAGCCAGGAGCTTCATTCAAACTATATCCGGTCGCCCTCACACCCAATCTATCACCAACAATATTTCCCCCAGGCCACCTATGGCAACTTTGCCTCGTACTCACCATCTTATCTTGGTAATGCCGTGATAACGAGTCTACTAAACCCAACGATTGGGATGTTTGGAGAAACAGTTTTCACTAGAATATTTGGTAGCGTGGATGGGAACTTATTGCCATACTCGCCATACAACAACTCAATCTCAGGAAATGCCAGTACGAGAATGAGAAGACAGGAAATGCAGCTTGATAAGTCTCTTAATAGAGTTTCCATCTTTCTATTTTGCTGCTTTATTATATGTCTTCTGTTATTTTGA

Protein sequence

MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPPSMNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF*

Homology

[Close]

BLAST of CSPI04G23560 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 5.7e-48
Identity = 122/267 (45.69%), Postives = 163/267 (61.05%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISE-DANGCFDCNICLDSAADPVVTLCG 60
           ME N+ +      +  +  + +   +++T  T  + + +GCFDCNICLD+A DPVVTLCG
Sbjct: 1   MEGNFFIRSDAQRAHDNGFIAKQKPNLTTAPTAGQANESGCFDCNICLDTAHDPVVTLCG 60

Query: 61  HLYCWPCIYKWLHVQISS-NEPENTQNCPVCKASITPSSLVPLYGRGTSNSDS---ESKK 120
           HL+CWPCIYKWLHVQ+SS +  ++  NCPVCK++IT +SLVPLYGRG S+  S     K+
Sbjct: 61  HLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSLVPLYGRGMSSPSSTFGSKKQ 120

Query: 121 SHLGMAVPRRPPPS-MNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNF 180
             L   +PRRP PS +  P    S+S+L PS +   +   SPS   ++ QY P+     F
Sbjct: 121 DALSTDIPRRPAPSALRNP--ITSASSLNPSLQ---HQTLSPS--FHNHQYSPR----GF 180

Query: 181 ASYSPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRR 240
            +   + L NAV+ S L P IGMFG+ V+TRIFG+    +    PY        S RM  
Sbjct: 181 TTTESTDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTNTIA--QPYQ-------SQRM-- 240

Query: 241 QEMQLDKSLNRVSIFLFCCFIICLLLF 262
             MQ +KSLNRVSIF  CC I+CLLLF
Sbjct: 241 --MQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of CSPI04G23560 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.0e-41
Identity = 102/250 (40.80%), Postives = 145/250 (58.00%), Query Frame = 0


Query: 24  WKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNEPENT 83
           WKS++ +  + E+ +G FDCNICLD   +PV+TLCGHLYCWPCIYKW++ Q  S+E  + 
Sbjct: 24  WKSMNDE--VEENISGGFDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQ 83

Query: 84  Q--NCPVCKASITPSSLVPLYGR-GTSNSDSESKKSHLGMAVPRRPPPSMNTPSHSNSSS 143
           Q   CPVCKA ++  +L+PLYGR G S   SE K  +LG+ +P+RP      PS      
Sbjct: 84  QQPQCPVCKAEVSEKTLIPLYGRGGQSTKPSEGKAPNLGIVIPQRP------PSPRCGGH 143

Query: 144 ALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPSYLGNAVITSLLNP------- 203
            L P+ +       +PS  +  + Y  Q+      +  P+Y G+ + + +L+P       
Sbjct: 144 FLLPTTD------SNPSQLLQRRGYQQQS-----QTRQPAYQGSYMSSPMLSPGGATANM 203

Query: 204 -TIGMFGETVFTRIFGSVDGNLLPY-SPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLF 262
               M GE  + RIFG+    +  Y + YN +IS  +S RMRRQ  Q D+SL R+  FLF
Sbjct: 204 LQHSMIGEVAYARIFGNSSTTMYTYPNSYNLAIS--SSPRMRRQLSQADRSLGRICFFLF 252

[Close]

BLAST of CSPI04G23560 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.8e-39
Identity = 103/241 (42.74%), Postives = 146/241 (60.58%), Query Frame = 0


Query: 26  SISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQ--ISSNEPENT 85
           ++S  S  ++D+N  FDCNICLDS  +PVVTLCGHL+CWPCI+KWL VQ   +S+E +  
Sbjct: 33  TVSVPSDDTDDSN--FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRH 92

Query: 86  QNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPP---SMNTPSHSNSSS 145
           + CPVCK+ ++ S+LVPLYGRG   +  E K S     VP+RP      +  P+   +S+
Sbjct: 93  RQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYAST 152

Query: 146 ALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPSYLGNAVITSLLNPTIGMFGE 205
            L  SQ +H N   SP      + Y+P +  G  +S S SY      +++L+P + M GE
Sbjct: 153 DLRLSQRVHFN---SP-----QEGYYPVS--GVMSSNSLSY------SAVLDPVMVMVGE 212

Query: 206 TVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLL 262
            V TR+FG+   +   Y P   +++G +  RMRR+ MQ DKSL R+  F  CC ++CLLL
Sbjct: 213 MVATRLFGTRVMDRFAY-PDTYNLAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLL 249

[Close]

BLAST of CSPI04G23560 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.1e-26
Identity = 78/233 (33.48%), Postives = 107/233 (45.92%), Query Frame = 0


Query: 36  DANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNE-------PENTQNCPV 95
           D+ G FDCNICLD   DPVVTLCGHL+CWPCI+KW +   +S +             CPV
Sbjct: 14  DSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPV 73

Query: 96  CKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPPSMNTPSHSNSSSALYPSQEL 155
           CK+ ++ ++LVP+YGRG                  + P    N PS              
Sbjct: 74  CKSDVSEATLVPIYGRG-----------------QKAPQSGSNVPSR------------- 133

Query: 156 HSNYIRSPSHPIYHQQYFPQATYGNFASYSPSYLGNAVITSLLNPTIGMFGETVFTRIFG 215
                  P+ P+Y  +   Q   G   S    Y        + +P +G+  E V+ R+FG
Sbjct: 134 -------PTGPVYDLRGVGQ-RLGEGESQRYMY-------RMPDPVMGVVCEMVYRRLFG 193

Query: 216 SVDGNLLPYSPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 262
               N+ PY   N         R RR+ MQ ++SL+RV +FL C   +CL LF
Sbjct: 194 ESSSNMAPYRDMN--------VRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of CSPI04G23560 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.2e-18
Identity = 46/103 (44.66%), Postives = 64/103 (62.14%), Query Frame = 0


Query: 31  STISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNEPENTQNCPVCK 90
           S+ ++D +  F+CNICLD+A D VV+LCGHL+CWPC+ +WL  +       N Q CPVCK
Sbjct: 14  SSSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTR------PNNQVCPVCK 73

Query: 91  ASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPPSMNTP 134
           ++I  + +VP+YGRG  +SD   K       VP RP    + P
Sbjct: 74  SAIDGNKVVPIYGRGGDSSDPRKK-------VPPRPKGQRSEP 103

[Close]

BLAST of CSPI04G23560 vs. ExPASy TrEMBL
Match: A0A0A0KZN0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G637750 PE=4 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 5.5e-147
Identity = 260/261 (99.62%), Postives = 260/261 (99.62%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM
Sbjct: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120

Query: 121 AVPRRPPPSMNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180
           AVPRRPPPSMNTPSHSNSSS LYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS
Sbjct: 121 AVPRRPPPSMNTPSHSNSSSTLYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180

Query: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240
           YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD
Sbjct: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240

Query: 241 KSLNRVSIFLFCCFIICLLLF 262
           KSLNRVSIFLFCCFIICLLLF
Sbjct: 241 KSLNRVSIFLFCCFIICLLLF 261

[Close]

BLAST of CSPI04G23560 vs. ExPASy TrEMBL
Match: A0A5A7VD79 (E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003900 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 9.1e-142
Identity = 254/261 (97.32%), Postives = 256/261 (98.08%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQNYSVPEAYLESEQDVSLKQNWKSIST+STISEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTESTISEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM
Sbjct: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120

Query: 121 AVPRRPPPSMNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180
           AVPRRPPPSMNTP  SNSSSA YPSQELHSNYIRSPSHPIYHQQYFPQA YGNFASY+PS
Sbjct: 121 AVPRRPPPSMNTPPPSNSSSASYPSQELHSNYIRSPSHPIYHQQYFPQA-YGNFASYAPS 180

Query: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240
           YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPY PYNNSISGNASTRMRRQEMQLD
Sbjct: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLD 240

Query: 241 KSLNRVSIFLFCCFIICLLLF 262
           KSLNRVSIFLFCCFIICLLLF
Sbjct: 241 KSLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of CSPI04G23560 vs. ExPASy TrEMBL
Match: A0A1S3BTX0 (E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo OX=3656 GN=LOC103493490 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 9.1e-142
Identity = 254/261 (97.32%), Postives = 256/261 (98.08%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQNYSVPEAYLESEQDVSLKQNWKSIST+STISEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTESTISEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM
Sbjct: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120

Query: 121 AVPRRPPPSMNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180
           AVPRRPPPSMNTP  SNSSSA YPSQELHSNYIRSPSHPIYHQQYFPQA YGNFASY+PS
Sbjct: 121 AVPRRPPPSMNTPPPSNSSSASYPSQELHSNYIRSPSHPIYHQQYFPQA-YGNFASYAPS 180

Query: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240
           YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPY PYNNSISGNASTRMRRQEMQLD
Sbjct: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLD 240

Query: 241 KSLNRVSIFLFCCFIICLLLF 262
           KSLNRVSIFLFCCFIICLLLF
Sbjct: 241 KSLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of CSPI04G23560 vs. ExPASy TrEMBL
Match: A0A6J1CDN1 (E3 ubiquitin-protein ligase RMA3-like OS=Momordica charantia OX=3673 GN=LOC111010508 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 7.0e-126
Identity = 226/262 (86.26%), Postives = 244/262 (93.13%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQ Y+ PEAY ESEQDVSLKQNWKSIS+Q T+S+DA+GCFDCNICLDSAA+PVVTLCGH
Sbjct: 1   MEQTYTTPEAYFESEQDVSLKQNWKSISSQPTVSDDAHGCFDCNICLDSAAEPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWL+VQISSNEPEN QNCPVCKASITPSSLVPLYGRG SN+DSESKKSHLG+
Sbjct: 61  LYCWPCIYKWLNVQISSNEPENVQNCPVCKASITPSSLVPLYGRGKSNTDSESKKSHLGV 120

Query: 121 AVPRRPPPS-MNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSP 180
           AVPRRPPPS  NT  +SN++SAL+PSQ+LH NY RSPSHPIYHQQYFPQA YG+ A+Y+P
Sbjct: 121 AVPRRPPPSATNTLHNSNATSALHPSQDLHPNYTRSPSHPIYHQQYFPQA-YGDIATYTP 180

Query: 181 SYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQL 240
           SYLGNAVITSLLNPTIGMFGETVFTRIFGSVD NLLPY PYNNSI GN S+RMRRQEMQL
Sbjct: 181 SYLGNAVITSLLNPTIGMFGETVFTRIFGSVDSNLLPYPPYNNSIPGNGSSRMRRQEMQL 240

Query: 241 DKSLNRVSIFLFCCFIICLLLF 262
           DKSLNRVSIFLFCCFIICLLLF
Sbjct: 241 DKSLNRVSIFLFCCFIICLLLF 261

[Close]

BLAST of CSPI04G23560 vs. ExPASy TrEMBL
Match: A0A6J1J1I0 (E3 ubiquitin-protein ligase RMA3-like OS=Cucurbita maxima OX=3661 GN=LOC111480472 PE=4 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.2e-125
Identity = 221/261 (84.67%), Postives = 239/261 (91.57%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           M+Q YS  EAYLESE DVSLKQNWKS+S QST+SEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MDQTYSASEAYLESEHDVSLKQNWKSMSAQSTVSEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           L+CWPCIYKW HVQISSNE ENT NCPVCKASIT SSLVPLYGRG SNSDSESKKSHLG+
Sbjct: 61  LFCWPCIYKWFHVQISSNETENTHNCPVCKASITLSSLVPLYGRGMSNSDSESKKSHLGI 120

Query: 121 AVPRRPPPSMNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180
            +PRRPPPSMNTP HSN++SAL+PS+ELH NYIRSP H IYHQQYFPQA YGNFA Y+PS
Sbjct: 121 TIPRRPPPSMNTPPHSNAASALHPSEELHPNYIRSPLHRIYHQQYFPQA-YGNFAPYAPS 180

Query: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240
           YLGNA++TSLLNPTIG+ GETV+TRIFGS DGNLLPY PYN+S++GN  TRMRRQEMQLD
Sbjct: 181 YLGNAMVTSLLNPTIGLIGETVYTRIFGSADGNLLPYPPYNHSVAGNIGTRMRRQEMQLD 240

Query: 241 KSLNRVSIFLFCCFIICLLLF 262
           KSLNRVSIFLFCCFIICLLLF
Sbjct: 241 KSLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of CSPI04G23560 vs. NCBI nr
Match: XP_004141226.1 (E3 ubiquitin-protein ligase RMA3 [Cucumis sativus] >KGN55135.1 hypothetical protein Csa_012256 [Cucumis sativus])

HSP 1 Score: 530.0 bits (1364), Expect = 1.1e-146
Identity = 260/261 (99.62%), Postives = 260/261 (99.62%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM
Sbjct: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120

Query: 121 AVPRRPPPSMNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180
           AVPRRPPPSMNTPSHSNSSS LYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS
Sbjct: 121 AVPRRPPPSMNTPSHSNSSSTLYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180

Query: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240
           YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD
Sbjct: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240

Query: 241 KSLNRVSIFLFCCFIICLLLF 262
           KSLNRVSIFLFCCFIICLLLF
Sbjct: 241 KSLNRVSIFLFCCFIICLLLF 261

[Close]

BLAST of CSPI04G23560 vs. NCBI nr
Match: XP_008452467.1 (PREDICTED: E3 ubiquitin-protein ligase RMA3 [Cucumis melo] >KAA0064336.1 E3 ubiquitin-protein ligase RMA3 [Cucumis melo var. makuwa] >TYK20251.1 E3 ubiquitin-protein ligase RMA3 [Cucumis melo var. makuwa])

HSP 1 Score: 512.7 bits (1319), Expect = 1.9e-141
Identity = 254/261 (97.32%), Postives = 256/261 (98.08%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQNYSVPEAYLESEQDVSLKQNWKSIST+STISEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTESTISEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM
Sbjct: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120

Query: 121 AVPRRPPPSMNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180
           AVPRRPPPSMNTP  SNSSSA YPSQELHSNYIRSPSHPIYHQQYFPQA YGNFASY+PS
Sbjct: 121 AVPRRPPPSMNTPPPSNSSSASYPSQELHSNYIRSPSHPIYHQQYFPQA-YGNFASYAPS 180

Query: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240
           YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPY PYNNSISGNASTRMRRQEMQLD
Sbjct: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEMQLD 240

Query: 241 KSLNRVSIFLFCCFIICLLLF 262
           KSLNRVSIFLFCCFIICLLLF
Sbjct: 241 KSLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of CSPI04G23560 vs. NCBI nr
Match: XP_038899693.1 (E3 ubiquitin-protein ligase RMA3 [Benincasa hispida])

HSP 1 Score: 497.3 bits (1279), Expect = 8.2e-137
Identity = 245/265 (92.45%), Postives = 254/265 (95.85%), Query Frame = 0


Query: 1   MEQNYSVP----EAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVT 60
           MEQNYSVP    EAYLESEQDVSLKQNWKSISTQST+SEDANGCFDCNICLDSAA+PVVT
Sbjct: 1   MEQNYSVPHSVSEAYLESEQDVSLKQNWKSISTQSTVSEDANGCFDCNICLDSAAEPVVT 60

Query: 61  LCGHLYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120
           LCGHLYCWPCIYKWLHVQISSN PENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS
Sbjct: 61  LCGHLYCWPCIYKWLHVQISSNRPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120

Query: 121 HLGMAVPRRPPPSMNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFAS 180
           HLGM VPRRPPPSMNTP HSN++SAL+PSQELH NY+RSPSHPIYHQQYFPQA YG+FAS
Sbjct: 121 HLGMVVPRRPPPSMNTPPHSNATSALHPSQELHPNYVRSPSHPIYHQQYFPQA-YGDFAS 180

Query: 181 YSPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQE 240
           Y+PSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPY PYNN+I GNASTRMRRQE
Sbjct: 181 YAPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQE 240

Query: 241 MQLDKSLNRVSIFLFCCFIICLLLF 262
           MQLDKSLNRVSIFLFCCFIICLLLF
Sbjct: 241 MQLDKSLNRVSIFLFCCFIICLLLF 264

[Close]

BLAST of CSPI04G23560 vs. NCBI nr
Match: XP_023521850.1 (E3 ubiquitin-protein ligase RMA3-like [Cucurbita pepo subsp. pepo] >XP_023521858.1 E3 ubiquitin-protein ligase RMA3-like [Cucurbita pepo subsp. pepo] >KAG6607875.1 E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037404.1 E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 464.9 bits (1195), Expect = 4.5e-127
Identity = 224/261 (85.82%), Postives = 241/261 (92.34%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           M+Q YS PEAYLESEQDVSLKQNWKS+S QST+SEDANGCFDCNICLDSAADPVVTLCGH
Sbjct: 1   MDQTYSSPEAYLESEQDVSLKQNWKSMSAQSTVSEDANGCFDCNICLDSAADPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           L+CWPCIYKW HVQISSNE ENT NCPVCKASITPSSLVPLYGRG S SDSESKKSHLG+
Sbjct: 61  LFCWPCIYKWFHVQISSNETENTHNCPVCKASITPSSLVPLYGRGKSYSDSESKKSHLGI 120

Query: 121 AVPRRPPPSMNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPS 180
            VPRRPPPSMNTP HSN++SAL+PS+ELH NYIRSP H IYHQQYFPQA YGNFA Y+PS
Sbjct: 121 TVPRRPPPSMNTPPHSNAASALHPSEELHPNYIRSPLHRIYHQQYFPQA-YGNFAPYAPS 180

Query: 181 YLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLD 240
           YLGNA++TSLLNPTIG+ GETV+TRIFGS DGNLLPY PYN+S++GN  TRMRRQEMQLD
Sbjct: 181 YLGNAMVTSLLNPTIGLIGETVYTRIFGSADGNLLPYPPYNHSVAGNVGTRMRRQEMQLD 240

Query: 241 KSLNRVSIFLFCCFIICLLLF 262
           KSLNRVSIFLFCCFIICLLLF
Sbjct: 241 KSLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of CSPI04G23560 vs. NCBI nr
Match: XP_022139659.1 (E3 ubiquitin-protein ligase RMA3-like [Momordica charantia])

HSP 1 Score: 459.9 bits (1182), Expect = 1.5e-125
Identity = 226/262 (86.26%), Postives = 244/262 (93.13%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVTLCGH 60
           MEQ Y+ PEAY ESEQDVSLKQNWKSIS+Q T+S+DA+GCFDCNICLDSAA+PVVTLCGH
Sbjct: 1   MEQTYTTPEAYFESEQDVSLKQNWKSISSQPTVSDDAHGCFDCNICLDSAAEPVVTLCGH 60

Query: 61  LYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGM 120
           LYCWPCIYKWL+VQISSNEPEN QNCPVCKASITPSSLVPLYGRG SN+DSESKKSHLG+
Sbjct: 61  LYCWPCIYKWLNVQISSNEPENVQNCPVCKASITPSSLVPLYGRGKSNTDSESKKSHLGV 120

Query: 121 AVPRRPPPS-MNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSP 180
           AVPRRPPPS  NT  +SN++SAL+PSQ+LH NY RSPSHPIYHQQYFPQA YG+ A+Y+P
Sbjct: 121 AVPRRPPPSATNTLHNSNATSALHPSQDLHPNYTRSPSHPIYHQQYFPQA-YGDIATYTP 180

Query: 181 SYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQL 240
           SYLGNAVITSLLNPTIGMFGETVFTRIFGSVD NLLPY PYNNSI GN S+RMRRQEMQL
Sbjct: 181 SYLGNAVITSLLNPTIGMFGETVFTRIFGSVDSNLLPYPPYNNSIPGNGSSRMRRQEMQL 240

Query: 241 DKSLNRVSIFLFCCFIICLLLF 262
           DKSLNRVSIFLFCCFIICLLLF
Sbjct: 241 DKSLNRVSIFLFCCFIICLLLF 261

[Close]

BLAST of CSPI04G23560 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 192.6 bits (488), Expect = 4.0e-49
Identity = 122/267 (45.69%), Postives = 163/267 (61.05%), Query Frame = 0


Query: 1   MEQNYSVPEAYLESEQDVSLKQNWKSISTQSTISE-DANGCFDCNICLDSAADPVVTLCG 60
           ME N+ +      +  +  + +   +++T  T  + + +GCFDCNICLD+A DPVVTLCG
Sbjct: 1   MEGNFFIRSDAQRAHDNGFIAKQKPNLTTAPTAGQANESGCFDCNICLDTAHDPVVTLCG 60

Query: 61  HLYCWPCIYKWLHVQISS-NEPENTQNCPVCKASITPSSLVPLYGRGTSNSDS---ESKK 120
           HL+CWPCIYKWLHVQ+SS +  ++  NCPVCK++IT +SLVPLYGRG S+  S     K+
Sbjct: 61  HLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSLVPLYGRGMSSPSSTFGSKKQ 120

Query: 121 SHLGMAVPRRPPPS-MNTPSHSNSSSALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNF 180
             L   +PRRP PS +  P    S+S+L PS +   +   SPS   ++ QY P+     F
Sbjct: 121 DALSTDIPRRPAPSALRNP--ITSASSLNPSLQ---HQTLSPS--FHNHQYSPR----GF 180

Query: 181 ASYSPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRR 240
            +   + L NAV+ S L P IGMFG+ V+TRIFG+    +    PY        S RM  
Sbjct: 181 TTTESTDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTNTIA--QPYQ-------SQRM-- 240

Query: 241 QEMQLDKSLNRVSIFLFCCFIICLLLF 262
             MQ +KSLNRVSIF  CC I+CLLLF
Sbjct: 241 --MQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of CSPI04G23560 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 163.7 bits (413), Expect = 2.0e-40
Identity = 103/241 (42.74%), Postives = 146/241 (60.58%), Query Frame = 0


Query: 26  SISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQ--ISSNEPENT 85
           ++S  S  ++D+N  FDCNICLDS  +PVVTLCGHL+CWPCI+KWL VQ   +S+E +  
Sbjct: 33  TVSVPSDDTDDSN--FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRH 92

Query: 86  QNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPP---SMNTPSHSNSSS 145
           + CPVCK+ ++ S+LVPLYGRG   +  E K S     VP+RP      +  P+   +S+
Sbjct: 93  RQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYAST 152

Query: 146 ALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPSYLGNAVITSLLNPTIGMFGE 205
            L  SQ +H N   SP      + Y+P +  G  +S S SY      +++L+P + M GE
Sbjct: 153 DLRLSQRVHFN---SP-----QEGYYPVS--GVMSSNSLSY------SAVLDPVMVMVGE 212

Query: 206 TVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLL 262
            V TR+FG+   +   Y P   +++G +  RMRR+ MQ DKSL R+  F  CC ++CLLL
Sbjct: 213 MVATRLFGTRVMDRFAY-PDTYNLAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLL 249

[Close]

BLAST of CSPI04G23560 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 163.7 bits (413), Expect = 2.0e-40
Identity = 103/241 (42.74%), Postives = 146/241 (60.58%), Query Frame = 0


Query: 26  SISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQ--ISSNEPENT 85
           ++S  S  ++D+N  FDCNICLDS  +PVVTLCGHL+CWPCI+KWL VQ   +S+E +  
Sbjct: 33  TVSVPSDDTDDSN--FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRH 92

Query: 86  QNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPP---SMNTPSHSNSSS 145
           + CPVCK+ ++ S+LVPLYGRG   +  E K S     VP+RP      +  P+   +S+
Sbjct: 93  RQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYAST 152

Query: 146 ALYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPSYLGNAVITSLLNPTIGMFGE 205
            L  SQ +H N   SP      + Y+P +  G  +S S SY      +++L+P + M GE
Sbjct: 153 DLRLSQRVHFN---SP-----QEGYYPVS--GVMSSNSLSY------SAVLDPVMVMVGE 212

Query: 206 TVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLL 262
            V TR+FG+   +   Y P   +++G +  RMRR+ MQ DKSL R+  F  CC ++CLLL
Sbjct: 213 MVATRLFGTRVMDRFAY-PDTYNLAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLL 249

[Close]

BLAST of CSPI04G23560 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-27
Identity = 78/233 (33.48%), Postives = 107/233 (45.92%), Query Frame = 0


Query: 36  DANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNE-------PENTQNCPV 95
           D+ G FDCNICLD   DPVVTLCGHL+CWPCI+KW +   +S +             CPV
Sbjct: 14  DSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPV 73

Query: 96  CKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPPSMNTPSHSNSSSALYPSQEL 155
           CK+ ++ ++LVP+YGRG                  + P    N PS              
Sbjct: 74  CKSDVSEATLVPIYGRG-----------------QKAPQSGSNVPSR------------- 133

Query: 156 HSNYIRSPSHPIYHQQYFPQATYGNFASYSPSYLGNAVITSLLNPTIGMFGETVFTRIFG 215
                  P+ P+Y  +   Q   G   S    Y        + +P +G+  E V+ R+FG
Sbjct: 134 -------PTGPVYDLRGVGQ-RLGEGESQRYMY-------RMPDPVMGVVCEMVYRRLFG 193

Query: 216 SVDGNLLPYSPYNNSISGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 262
               N+ PY   N         R RR+ MQ ++SL+RV +FL C   +CL LF
Sbjct: 194 ESSSNMAPYRDMN--------VRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of CSPI04G23560 vs. TAIR 10
Match: AT2G23780.1 (RING/U-box superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 1.4e-25
Identity = 79/238 (33.19%), Postives = 113/238 (47.48%), Query Frame = 0


Query: 26  SISTQSTISEDANGCFDCNICLDSAADPVVTLCGHLYCWPCIYKWLHVQISSNEPENTQN 85
           S S  +  + D  G F+CNIC + A DP+VTLCGHL+CWPC+Y+WLH         ++Q 
Sbjct: 11  SYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLH------HHSHSQE 70

Query: 86  CPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMAVPRRPPPSMNTPSHSNSSSALYPS 145
           CPVCKA +    LVPLYGRG + +D  SK+ + G+ +P RP            ++A  P 
Sbjct: 71  CPVCKAVVQDDKLVPLYGRGKNQTDPRSKR-YPGLRIPNRP------TGQRPETAAPPPQ 130

Query: 146 QELHSNYIRSPSHPIYHQQYFPQATYGNFASYSPSYLGNAVITSLLNPTIGMFGETVFTR 205
            E  SN+       +      P      F ++S  + G  ++ SL N     F +     
Sbjct: 131 PEAASNFFNYGIGLM--GGIMPMMATTRFGNFSMGFGG--LLPSLFNFQFHGFHDAT--- 190

Query: 206 IFGSVDGNLLPYSPYNNSISG----NASTRMRRQEMQLDKSLNRVSIFLFCCFIICLL 260
           ++GS  G   PY  Y+N   G         M R   Q D  L  +  F+  C +I L+
Sbjct: 191 LYGSTPG--YPYGGYHNGFRGVPPRGQERPMARGGNQSDAFLKNILFFVGICVVIFLI 226

The following BLAST results are available for this feature:

BLAST of CSPI04G23560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GUK7	5.7e-48	45.69	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV...	[more]
Q6R567	1.0e-41	40.80	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=...	[more]
O64425	2.8e-39	42.74	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV...	[more]
P93030	2.1e-26	33.48	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV...	[more]
Q09463	1.2e-18	44.66	E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1	[more]

BLAST of CSPI04G23560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZN0	5.5e-147	99.62	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G637750 P...	[more]
A0A5A7VD79	9.1e-142	97.32	E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BTX0	9.1e-142	97.32	E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo OX=3656 GN=LOC103493490 PE=4 SV...	[more]
A0A6J1CDN1	7.0e-126	86.26	E3 ubiquitin-protein ligase RMA3-like OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1J1I0	1.2e-125	84.67	E3 ubiquitin-protein ligase RMA3-like OS=Cucurbita maxima OX=3661 GN=LOC11148047...	[more]

BLAST of CSPI04G23560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141226.1	1.1e-146	99.62	E3 ubiquitin-protein ligase RMA3 [Cucumis sativus] >KGN55135.1 hypothetical prot...	[more]
XP_008452467.1	1.9e-141	97.32	PREDICTED: E3 ubiquitin-protein ligase RMA3 [Cucumis melo] >KAA0064336.1 E3 ubiq...	[more]
XP_038899693.1	8.2e-137	92.45	E3 ubiquitin-protein ligase RMA3 [Benincasa hispida]	[more]
XP_023521850.1	4.5e-127	85.82	E3 ubiquitin-protein ligase RMA3-like [Cucurbita pepo subsp. pepo] >XP_023521858...	[more]
XP_022139659.1	1.5e-125	86.26	E3 ubiquitin-protein ligase RMA3-like [Momordica charantia]	[more]

BLAST of CSPI04G23560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27470.1	4.0e-49	45.69	RING membrane-anchor 3	[more]
AT4G03510.1	2.0e-40	42.74	RING membrane-anchor 1	[more]
AT4G03510.2	2.0e-40	42.74	RING membrane-anchor 1	[more]
AT4G28270.1	1.5e-27	33.48	RING membrane-anchor 2	[more]
AT2G23780.1	1.4e-25	33.19	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 43..89 e-value: 6.6E-10 score: 48.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 43..90 score: 12.872856
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 3..106 e-value: 9.7E-18 score: 65.9
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 43..89 e-value: 1.1E-8 score: 34.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 104..142
None	No IPR available	PANTHER	PTHR12313:SF69	E3 UBIQUITIN-PROTEIN LIGASE RMA3-LIKE	coord: 2..261
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 41..91 e-value: 1.43174E-25 score: 93.1375
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 23..96
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..261
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 58..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G23560.1	CSPI04G23560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity