CSPI04G23330 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G23330
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionpre-mRNA-splicing factor CWC22 homolog
LocationChr4: 21436170 .. 21437088 (-)
RNA-Seq ExpressionCSPI04G23330
SyntenyCSPI04G23330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAACGGACACTTCATGCAAACCAAATGACGATGGAGTCTCGCCGGGAAAATGGCTCCTCCGCTGAGATTTTTTCCTTTCCGAGCACTCCAGTTCTGGATCAGGATTCCGATTTCGAATTTGGATCCATTACTCCTGATTCTCCTTCTACTTTCAACAATTCTCCCGCTGATCATTTGTTCGTTAATGGCCGTCTCTTGCCTCACTCTTTTCCGCCACAGCCGATTAGTGTTTACGCTGTTGATCTTGCTTCTCGTCATACCAGCAGAACGAGCAGTGTCGGGAGTAAGGACTCGTTCATGTCGTCGCGAAGTAATAGCACTAATAGTAGGAGCAGTAGCAGTTGCGGCAGCAGTACTAGCGCGAGGACGAGTTCGAGCGATAATAGTAATAATAATAATAATTTGGAGATTAGATCCTCGAGATTCTCTTCGTATCAGAAGAAATCGCAGAAATCTTCTGTTTCCGCTCAAGTGTACGGATCGTGTCAGAGATGGCAGTTCATCGCATCGGTGTCTGTACCGACGTCGCTGAGTCGGGAGAATTCGAGAAGGAAGAAGTCTGTGGAGAAAATTGGGGGTAAATCGGATTTGAGGAGGAAGAAGAGGCGGGCGGAGAAAACATCGACGGCGAGGAAGAAGAAAAAGAAGAAGAAGAAGAAGAATATGTGGTTCGGAAGGAAGGTTTTCCGATGGATTATACTGGTTTGTAAGGAATGTCATGCGATCGAACCGTCGAGGAAGGATGAGATTGCGAGGAAGTCGAAATTGACGACGATGACGACCAAGCCGCAGAGTAATTGAATTTAATTATTATTGATTTATAGTTTCTTCTGGATTTTGCCTCTTGCTGATGATATATGATCTGGTTTTTCATCTATCTCTTAATTTCGGGAATTTACTTTTCTTCAATTGTTGGCG

mRNA sequence

GTAACGGACACTTCATGCAAACCAAATGACGATGGAGTCTCGCCGGGAAAATGGCTCCTCCGCTGAGATTTTTTCCTTTCCGAGCACTCCAGTTCTGGATCAGGATTCCGATTTCGAATTTGGATCCATTACTCCTGATTCTCCTTCTACTTTCAACAATTCTCCCGCTGATCATTTGTTCGTTAATGGCCGTCTCTTGCCTCACTCTTTTCCGCCACAGCCGATTAGTGTTTACGCTGTTGATCTTGCTTCTCGTCATACCAGCAGAACGAGCAGTGTCGGGAGTAAGGACTCGTTCATGTCGTCGCGAAGTAATAGCACTAATAGTAGGAGCAGTAGCAGTTGCGGCAGCAGTACTAGCGCGAGGACGAGTTCGAGCGATAATAGTAATAATAATAATAATTTGGAGATTAGATCCTCGAGATTCTCTTCGTATCAGAAGAAATCGCAGAAATCTTCTGTTTCCGCTCAAGTGTACGGATCGTGTCAGAGATGGCAGTTCATCGCATCGGTGTCTGTACCGACGTCGCTGAGTCGGGAGAATTCGAGAAGGAAGAAGTCTGTGGAGAAAATTGGGGGTAAATCGGATTTGAGGAGGAAGAAGAGGCGGGCGGAGAAAACATCGACGGCGAGGAAGAAGAAAAAGAAGAAGAAGAAGAAGAATATGTGGTTCGGAAGGAAGGTTTTCCGATGGATTATACTGGTTTGTAAGGAATGTCATGCGATCGAACCGTCGAGGAAGGATGAGATTGCGAGGAAGTCGAAATTGACGACGATGACGACCAAGCCGCAGAGTAATTGAATTTAATTATTATTGATTTATAGTTTCTTCTGGATTTTGCCTCTTGCTGATGATATATGATCTGGTTTTTCATCTATCTCTTAATTTCGGGAATTTACTTTTCTTCAATTGTTGGCG

Coding sequence (CDS)

ATGACGATGGAGTCTCGCCGGGAAAATGGCTCCTCCGCTGAGATTTTTTCCTTTCCGAGCACTCCAGTTCTGGATCAGGATTCCGATTTCGAATTTGGATCCATTACTCCTGATTCTCCTTCTACTTTCAACAATTCTCCCGCTGATCATTTGTTCGTTAATGGCCGTCTCTTGCCTCACTCTTTTCCGCCACAGCCGATTAGTGTTTACGCTGTTGATCTTGCTTCTCGTCATACCAGCAGAACGAGCAGTGTCGGGAGTAAGGACTCGTTCATGTCGTCGCGAAGTAATAGCACTAATAGTAGGAGCAGTAGCAGTTGCGGCAGCAGTACTAGCGCGAGGACGAGTTCGAGCGATAATAGTAATAATAATAATAATTTGGAGATTAGATCCTCGAGATTCTCTTCGTATCAGAAGAAATCGCAGAAATCTTCTGTTTCCGCTCAAGTGTACGGATCGTGTCAGAGATGGCAGTTCATCGCATCGGTGTCTGTACCGACGTCGCTGAGTCGGGAGAATTCGAGAAGGAAGAAGTCTGTGGAGAAAATTGGGGGTAAATCGGATTTGAGGAGGAAGAAGAGGCGGGCGGAGAAAACATCGACGGCGAGGAAGAAGAAAAAGAAGAAGAAGAAGAAGAATATGTGGTTCGGAAGGAAGGTTTTCCGATGGATTATACTGGTTTGTAAGGAATGTCATGCGATCGAACCGTCGAGGAAGGATGAGATTGCGAGGAAGTCGAAATTGACGACGATGACGACCAAGCCGCAGAGTAATTGA

Protein sequence

MTMESRRENGSSAEIFSFPSTPVLDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPHSFPPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDNSNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVEKIGGKSDLRRKKRRAEKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKDEIARKSKLTTMTTKPQSN*
Homology
BLAST of CSPI04G23330 vs. ExPASy TrEMBL
Match: A0A0A0L251 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G631560 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 4.8e-127
Identity = 257/258 (99.61%), Postives = 257/258 (99.61%), Query Frame = 0

Query: 1   MTMESRRENGSSAEIFSFPSTPVLDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 60
           MTMESRRENGSSAEIFSFPSTPVLDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH
Sbjct: 1   MTMESRRENGSSAEIFSFPSTPVLDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 60

Query: 61  SFPPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDN 120
           SFPPQPISVYAVDLASR TSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDN
Sbjct: 61  SFPPQPISVYAVDLASRQTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDN 120

Query: 121 SNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSV 180
           SNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSV
Sbjct: 121 SNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSV 180

Query: 181 EKIGGKSDLRRKKRRAEKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD 240
           EKIGGKSDLRRKKRRAEKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD
Sbjct: 181 EKIGGKSDLRRKKRRAEKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD 240

Query: 241 EIARKSKLTTMTTKPQSN 259
           EIARKSKLTTMTTKPQSN
Sbjct: 241 EIARKSKLTTMTTKPQSN 258

BLAST of CSPI04G23330 vs. ExPASy TrEMBL
Match: A0A5D3DWZ9 (Putative membrane-associated kinase regulator 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001730 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.2e-117
Identity = 247/259 (95.37%), Postives = 251/259 (96.91%), Query Frame = 0

Query: 3   MESRRENGSSAEIFSFPSTPVLDQDS-DFEFGSITPDSPSTFNNSPADHLFVNGRLLPHS 62
           MESRRENGSSAEIFSFPSTPV DQDS DFEFGSITPDSPSTFNNSPADHLFVNGRLLPHS
Sbjct: 1   MESRRENGSSAEIFSFPSTPVQDQDSDDFEFGSITPDSPSTFNNSPADHLFVNGRLLPHS 60

Query: 63  FPPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDNS 122
           FPPQPISVYAVD+ASR TSRTSSVGSKDSF+SSRSNSTNSRSSSSCGSSTSARTSSSDN+
Sbjct: 61  FPPQPISVYAVDVASRQTSRTSSVGSKDSFLSSRSNSTNSRSSSSCGSSTSARTSSSDNN 120

Query: 123 NNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVE 182
           N+NNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVE
Sbjct: 121 NSNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVE 180

Query: 183 KIGGKSDLRRKKRRAEKTSTAR-KKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD 242
           KIGGKSDLRR KRR EKTSTAR KKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD
Sbjct: 181 KIGGKSDLRRNKRRTEKTSTARKKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD 240

Query: 243 EIARKSKL-TTMTTKPQSN 259
           EIARKSKL TT TTKPQSN
Sbjct: 241 EIARKSKLTTTTTTKPQSN 259

BLAST of CSPI04G23330 vs. ExPASy TrEMBL
Match: A0A1S3CKA5 (probable membrane-associated kinase regulator 1 OS=Cucumis melo OX=3656 GN=LOC103501769 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.2e-117
Identity = 247/259 (95.37%), Postives = 251/259 (96.91%), Query Frame = 0

Query: 3   MESRRENGSSAEIFSFPSTPVLDQDS-DFEFGSITPDSPSTFNNSPADHLFVNGRLLPHS 62
           MESRRENGSSAEIFSFPSTPV DQDS DFEFGSITPDSPSTFNNSPADHLFVNGRLLPHS
Sbjct: 1   MESRRENGSSAEIFSFPSTPVQDQDSDDFEFGSITPDSPSTFNNSPADHLFVNGRLLPHS 60

Query: 63  FPPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDNS 122
           FPPQPISVYAVD+ASR TSRTSSVGSKDSF+SSRSNSTNSRSSSSCGSSTSARTSSSDN+
Sbjct: 61  FPPQPISVYAVDVASRQTSRTSSVGSKDSFLSSRSNSTNSRSSSSCGSSTSARTSSSDNN 120

Query: 123 NNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVE 182
           N+NNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVE
Sbjct: 121 NSNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVE 180

Query: 183 KIGGKSDLRRKKRRAEKTSTAR-KKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD 242
           KIGGKSDLRR KRR EKTSTAR KKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD
Sbjct: 181 KIGGKSDLRRNKRRTEKTSTARKKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD 240

Query: 243 EIARKSKL-TTMTTKPQSN 259
           EIARKSKL TT TTKPQSN
Sbjct: 241 EIARKSKLTTTTTTKPQSN 259

BLAST of CSPI04G23330 vs. ExPASy TrEMBL
Match: A0A6J1FSH9 (pre-mRNA-splicing factor CWC22 homolog OS=Cucurbita moschata OX=3662 GN=LOC111446467 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.3e-92
Identity = 212/259 (81.85%), Postives = 224/259 (86.49%), Query Frame = 0

Query: 3   MESRRENGSSAEIFSFPSTPV--LDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 62
           MESRRE  SS EIFSFPSTPV   DQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH
Sbjct: 1   MESRREQSSSGEIFSFPSTPVQDQDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 60

Query: 63  SFPPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDN 122
           SFPPQPI VYAVD       RTSSVGSKDS MSSRSNSTNSRSSSSCGSSTSARTSSS++
Sbjct: 61  SFPPQPIGVYAVD-------RTSSVGSKDSIMSSRSNSTNSRSSSSCGSSTSARTSSSES 120

Query: 123 SNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSV 182
           +N +     RSSRFSS+QKKS KSSVSAQVYGS QRWQFIASV VPT+LSRENSRRKK+V
Sbjct: 121 NNVD-----RSSRFSSHQKKSPKSSVSAQVYGSSQRWQFIASVPVPTTLSRENSRRKKAV 180

Query: 183 EKIGGKSDLRRKKRRAEKTST-ARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRK 242
           EKIGGKSDLRR K+R EKT+   +KKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRK
Sbjct: 181 EKIGGKSDLRRNKKRIEKTTARKKKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRK 240

Query: 243 DEIA-RKSKLTTMTTKPQS 258
           DEI  RK+KL    TKP+S
Sbjct: 241 DEIVMRKTKL----TKPKS 243

BLAST of CSPI04G23330 vs. ExPASy TrEMBL
Match: A0A6J1J3G5 (pre-mRNA-splicing factor CWC22 homolog OS=Cucurbita maxima OX=3661 GN=LOC111480935 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 6.1e-90
Identity = 210/260 (80.77%), Postives = 222/260 (85.38%), Query Frame = 0

Query: 3   MESRRENGSSAEIFSFPSTPV--LDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 62
           MESRRE  SS EIFSFPSTPV   DQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH
Sbjct: 1   MESRREQSSSGEIFSFPSTPVQDQDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 60

Query: 63  SFPPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDN 122
           SFPPQPI VYAVD       RTSSVGSKDS MSSRSNSTNSRSSSSCGSSTSARTSSS++
Sbjct: 61  SFPPQPIGVYAVD-------RTSSVGSKDSIMSSRSNSTNSRSSSSCGSSTSARTSSSES 120

Query: 123 SNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSV 182
           +N +     RSSRFSS+QKKS KSSVSAQVYGS QRWQFIA  SVP +LSRENSRRKK+V
Sbjct: 121 NNVD-----RSSRFSSHQKKSPKSSVSAQVYGSSQRWQFIA--SVPATLSRENSRRKKAV 180

Query: 183 EKIGGKSDLRRKKRRAEKTST--ARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSR 242
           EKIGGKSDLRR K+R EKT+    +KKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSR
Sbjct: 181 EKIGGKSDLRRNKKRTEKTTARKKKKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSR 240

Query: 243 KDEIA-RKSKLTTMTTKPQS 258
           KDEI  RK+KL    TKP+S
Sbjct: 241 KDEIVMRKTKL----TKPKS 242

BLAST of CSPI04G23330 vs. NCBI nr
Match: XP_011654065.1 (probable membrane-associated kinase regulator 3 [Cucumis sativus] >KGN55114.1 hypothetical protein Csa_011965 [Cucumis sativus])

HSP 1 Score: 463.8 bits (1192), Expect = 9.9e-127
Identity = 257/258 (99.61%), Postives = 257/258 (99.61%), Query Frame = 0

Query: 1   MTMESRRENGSSAEIFSFPSTPVLDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 60
           MTMESRRENGSSAEIFSFPSTPVLDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH
Sbjct: 1   MTMESRRENGSSAEIFSFPSTPVLDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 60

Query: 61  SFPPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDN 120
           SFPPQPISVYAVDLASR TSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDN
Sbjct: 61  SFPPQPISVYAVDLASRQTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDN 120

Query: 121 SNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSV 180
           SNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSV
Sbjct: 121 SNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSV 180

Query: 181 EKIGGKSDLRRKKRRAEKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD 240
           EKIGGKSDLRRKKRRAEKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD
Sbjct: 181 EKIGGKSDLRRKKRRAEKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD 240

Query: 241 EIARKSKLTTMTTKPQSN 259
           EIARKSKLTTMTTKPQSN
Sbjct: 241 EIARKSKLTTMTTKPQSN 258

BLAST of CSPI04G23330 vs. NCBI nr
Match: XP_008463678.1 (PREDICTED: probable membrane-associated kinase regulator 1 [Cucumis melo] >KAA0066822.1 putative membrane-associated kinase regulator 1 [Cucumis melo var. makuwa] >TYK27969.1 putative membrane-associated kinase regulator 1 [Cucumis melo var. makuwa])

HSP 1 Score: 432.6 bits (1111), Expect = 2.5e-117
Identity = 247/259 (95.37%), Postives = 251/259 (96.91%), Query Frame = 0

Query: 3   MESRRENGSSAEIFSFPSTPVLDQDS-DFEFGSITPDSPSTFNNSPADHLFVNGRLLPHS 62
           MESRRENGSSAEIFSFPSTPV DQDS DFEFGSITPDSPSTFNNSPADHLFVNGRLLPHS
Sbjct: 1   MESRRENGSSAEIFSFPSTPVQDQDSDDFEFGSITPDSPSTFNNSPADHLFVNGRLLPHS 60

Query: 63  FPPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDNS 122
           FPPQPISVYAVD+ASR TSRTSSVGSKDSF+SSRSNSTNSRSSSSCGSSTSARTSSSDN+
Sbjct: 61  FPPQPISVYAVDVASRQTSRTSSVGSKDSFLSSRSNSTNSRSSSSCGSSTSARTSSSDNN 120

Query: 123 NNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVE 182
           N+NNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVE
Sbjct: 121 NSNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVE 180

Query: 183 KIGGKSDLRRKKRRAEKTSTAR-KKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD 242
           KIGGKSDLRR KRR EKTSTAR KKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD
Sbjct: 181 KIGGKSDLRRNKRRTEKTSTARKKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKD 240

Query: 243 EIARKSKL-TTMTTKPQSN 259
           EIARKSKL TT TTKPQSN
Sbjct: 241 EIARKSKLTTTTTTKPQSN 259

BLAST of CSPI04G23330 vs. NCBI nr
Match: XP_038899281.1 (probable membrane-associated kinase regulator 1 [Benincasa hispida])

HSP 1 Score: 401.7 bits (1031), Expect = 4.6e-108
Identity = 230/259 (88.80%), Postives = 237/259 (91.51%), Query Frame = 0

Query: 3   MESRRENGSSAEIFSFPSTPVLDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPHSF 62
           MESRRE  SS EIFSFPSTPV DQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPHSF
Sbjct: 1   MESRREKSSSGEIFSFPSTPVQDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPHSF 60

Query: 63  PPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDNSN 122
           PPQPI VY VD+ SR TSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSD  N
Sbjct: 61  PPQPIGVYTVDV-SRQTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSD--N 120

Query: 123 NNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSVEK 182
           NNNN++IRSSRFSSYQKKSQKSSVSAQVYGS QRWQFIASVSVP +LSRENSRRKKSVEK
Sbjct: 121 NNNNVDIRSSRFSSYQKKSQKSSVSAQVYGSSQRWQFIASVSVPVTLSRENSRRKKSVEK 180

Query: 183 IGGKSDLRRKKRRAEKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKDEI 242
           IGGKSDLRRKKRR EKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKDE+
Sbjct: 181 IGGKSDLRRKKRRTEKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRKDEV 240

Query: 243 ARKS----KLTTMTTKPQS 258
            RK     K  + TTKP+S
Sbjct: 241 VRKDEIVRKTKSTTTKPKS 256

BLAST of CSPI04G23330 vs. NCBI nr
Match: XP_023525336.1 (pre-mRNA-splicing factor CWC22 homolog [Cucurbita pepo subsp. pepo] >KAG6607885.1 hypothetical protein SDJN03_01227, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037411.1 hypothetical protein SDJN02_01037, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 349.0 bits (894), Expect = 3.6e-92
Identity = 212/259 (81.85%), Postives = 224/259 (86.49%), Query Frame = 0

Query: 3   MESRRENGSSAEIFSFPSTPV--LDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 62
           MESRRE  SS EIFSFPSTPV   DQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH
Sbjct: 1   MESRREQSSSGEIFSFPSTPVQDQDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 60

Query: 63  SFPPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDN 122
           SFPPQPI VYAVD       RTSSVGSKDS MSSRSNSTNSRSSSSCGSSTSARTSSS++
Sbjct: 61  SFPPQPIGVYAVD-------RTSSVGSKDSIMSSRSNSTNSRSSSSCGSSTSARTSSSES 120

Query: 123 SNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSV 182
           +N +     RSSRFSS+QKKS KSSVSAQVYGS QRWQFIASV VPT+LSRENSRRKK+V
Sbjct: 121 NNVD-----RSSRFSSHQKKSPKSSVSAQVYGSSQRWQFIASVPVPTTLSRENSRRKKAV 180

Query: 183 EKIGGKSDLRRKKRRAEKTST-ARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRK 242
           EKIGGKSDLRR K+R EKT+   +KKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRK
Sbjct: 181 EKIGGKSDLRRNKKRTEKTTARKKKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRK 240

Query: 243 DEIA-RKSKLTTMTTKPQS 258
           DEI  RK+KL    TKP+S
Sbjct: 241 DEIVMRKTKL----TKPKS 243

BLAST of CSPI04G23330 vs. NCBI nr
Match: XP_022941070.1 (pre-mRNA-splicing factor CWC22 homolog [Cucurbita moschata])

HSP 1 Score: 348.6 bits (893), Expect = 4.7e-92
Identity = 212/259 (81.85%), Postives = 224/259 (86.49%), Query Frame = 0

Query: 3   MESRRENGSSAEIFSFPSTPV--LDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 62
           MESRRE  SS EIFSFPSTPV   DQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH
Sbjct: 1   MESRREQSSSGEIFSFPSTPVQDQDQDSDFEFGSITPDSPSTFNNSPADHLFVNGRLLPH 60

Query: 63  SFPPQPISVYAVDLASRHTSRTSSVGSKDSFMSSRSNSTNSRSSSSCGSSTSARTSSSDN 122
           SFPPQPI VYAVD       RTSSVGSKDS MSSRSNSTNSRSSSSCGSSTSARTSSS++
Sbjct: 61  SFPPQPIGVYAVD-------RTSSVGSKDSIMSSRSNSTNSRSSSSCGSSTSARTSSSES 120

Query: 123 SNNNNNLEIRSSRFSSYQKKSQKSSVSAQVYGSCQRWQFIASVSVPTSLSRENSRRKKSV 182
           +N +     RSSRFSS+QKKS KSSVSAQVYGS QRWQFIASV VPT+LSRENSRRKK+V
Sbjct: 121 NNVD-----RSSRFSSHQKKSPKSSVSAQVYGSSQRWQFIASVPVPTTLSRENSRRKKAV 180

Query: 183 EKIGGKSDLRRKKRRAEKTST-ARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRK 242
           EKIGGKSDLRR K+R EKT+   +KKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRK
Sbjct: 181 EKIGGKSDLRRNKKRIEKTTARKKKKKKKKKKKNMWFGRKVFRWIILVCKECHAIEPSRK 240

Query: 243 DEIA-RKSKLTTMTTKPQS 258
           DEI  RK+KL    TKP+S
Sbjct: 241 DEIVMRKTKL----TKPKS 243

BLAST of CSPI04G23330 vs. TAIR 10
Match: AT3G15250.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G53163.1); Has 409 Blast hits to 218 proteins in 63 species: Archae - 0; Bacteria - 22; Metazoa - 178; Fungi - 77; Plants - 38; Viruses - 1; Other Eukaryotes - 93 (source: NCBI BLink). )

HSP 1 Score: 68.9 bits (167), Expect = 6.6e-12
Identity = 87/239 (36.40%), Postives = 118/239 (49.37%), Query Frame = 0

Query: 29  DFEFGSITPDSPST---FNNSPADHLFVNGRLLPHSFPPQPISVYAVDLASRHTSRTSSV 88
           +FEFGS+TP SPS      NSPADHLF NGRLLPH+FP           +S +T R  S+
Sbjct: 16  EFEFGSLTPTSPSQDSFSENSPADHLFFNGRLLPHAFPA---------ASSIYTKR--SI 75

Query: 89  GSKDSFMSSRSNSTNSRS---SSSCGSSTSARTSSSDNSNNNNNLE-------------- 148
            S  S  +SR NSTNS S   S++  +S S RTS S ++ NN+ +               
Sbjct: 76  SSTTSEYTSRCNSTNSSSSFGSNNSSTSQSPRTSYSTSNKNNSPINQEPLDLKLKPPISR 135

Query: 149 -------IRSSRFSSYQKKSQK----SSVSAQVYGSCQRWQFIASVSVPTSLS-RENSRR 208
                  + SS +SS Q K +K    ++ S     S QRWQ+I  V V  S   R   R+
Sbjct: 136 CDKTVNTMSSSSYSSPQHKRRKAMEMATTSLYRSYSSQRWQYITPVPVRMSGGIRVGGRK 195

Query: 209 KKSVEKIGGKSDLRRKKRRAEKTSTARKKKKKKKKKNMWFGRKVFRWIILVCKECHAIE 236
           KK+V          R +++ E+   +R  K       +W  RK+    I VC+ECHA+E
Sbjct: 196 KKAV----------RVRKKTEERRVSRVMK-------LW--RKLLMAAIFVCRECHALE 224

BLAST of CSPI04G23330 vs. TAIR 10
Match: AT1G53163.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G15250.1); Has 315 Blast hits to 216 proteins in 74 species: Archae - 0; Bacteria - 86; Metazoa - 43; Fungi - 52; Plants - 27; Viruses - 1; Other Eukaryotes - 106 (source: NCBI BLink). )

HSP 1 Score: 62.0 bits (149), Expect = 8.1e-10
Identity = 86/280 (30.71%), Postives = 129/280 (46.07%), Query Frame = 0

Query: 28  SDFEFGSITPDSPST---FNNSPADHLFVNGRLLPHSFPPQPISVYAVD-LASRHTSRTS 87
           SDFEFGS+TP SPS      NSPADHLF+NGRLLPHSFP   I+  +V    S +T R+ 
Sbjct: 26  SDFEFGSLTPTSPSQDPFSENSPADHLFLNGRLLPHSFPTSTITTRSVSRTTSENTFRSD 85

Query: 88  SVGSKDSFMSSRSNSTNSRSSSSCGSSTSART------------------------SSSD 147
           S  S+ S  SS ++ T   S+ +    TS+ T                        +SS 
Sbjct: 86  SWSSRSSDSSSPTSYTPRTSTCNIFKITSSSTGHGKETNITCFGTRSEEIMCLRPATSSK 145

Query: 148 NSNN----------------------NNNLEIRSSRFSSY----QKKSQKSSVSAQVYGS 207
            +N                        N   + ++   SY    ++K     V+AQ+YGS
Sbjct: 146 KTNTCISKEYYHKPSLILDLKKRPPVENGKAVNTTNKVSYLPQCKRKKATEIVTAQLYGS 205

Query: 208 -CQRWQFIASVSVPTSLSRENSRRKKSVE-KIGGKSDLRRKKRRAEKTSTARKKKKKKKK 249
             +RWQ I  V       RE S + K    K+GG+   ++K  R +K   A +++    K
Sbjct: 206 YSRRWQHITPV-----FKREGSVKSKGGGIKVGGR---KKKSTRRKKPKKAEERRGISSK 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0L2514.8e-12799.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G631560 PE=4 SV=1[more]
A0A5D3DWZ91.2e-11795.37Putative membrane-associated kinase regulator 1 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3CKA51.2e-11795.37probable membrane-associated kinase regulator 1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1FSH92.3e-9281.85pre-mRNA-splicing factor CWC22 homolog OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1J3G56.1e-9080.77pre-mRNA-splicing factor CWC22 homolog OS=Cucurbita maxima OX=3661 GN=LOC1114809... [more]
Match NameE-valueIdentityDescription
XP_011654065.19.9e-12799.61probable membrane-associated kinase regulator 3 [Cucumis sativus] >KGN55114.1 hy... [more]
XP_008463678.12.5e-11795.37PREDICTED: probable membrane-associated kinase regulator 1 [Cucumis melo] >KAA00... [more]
XP_038899281.14.6e-10888.80probable membrane-associated kinase regulator 1 [Benincasa hispida][more]
XP_023525336.13.6e-9281.85pre-mRNA-splicing factor CWC22 homolog [Cucurbita pepo subsp. pepo] >KAG6607885.... [more]
XP_022941070.14.7e-9281.85pre-mRNA-splicing factor CWC22 homolog [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G15250.16.6e-1236.40unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G53163.18.1e-1030.71unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..212
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 188..212
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 171..187
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..127

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G23330.1CSPI04G23330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0016310 phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0016301 kinase activity
molecular_function GO:0019210 kinase inhibitor activity