CSPI04G22910 (gene) Cucumber (PI 183967) v1

BLAST of CSPI04G22910 vs. ExPASy Swiss-Prot
Match: P81898 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Prunus dulcis OX=3755 PE=1 SV=2)

HSP 1 Score: 746.5 bits (1926), Expect = 2.4e-214
Identity = 374/563 (66.43%), Postives = 435/563 (77.26%), Query Frame = 0

Query: 48  HSTPPTVFFEVSKPIASPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIV 107
           H TPPTVFFEV+KPI  PK+KPCS L+L HDFA+TYG+ PV A+YTPPS C    FS IV
Sbjct: 6   HDTPPTVFFEVTKPIEVPKTKPCSQLILQHDFAYTYGQAPVFANYTPPSDCPSQTFSTIV 65

Query: 108 LEWKATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLA 167
           LEWKATCR RQFDRIFGVWLGGVEILRSCTAEPR  GIVWTV+KDITRY SLL  NQTLA
Sbjct: 66  LEWKATCRRRQFDRIFGVWLGGVEILRSCTAEPRPNGIVWTVEKDITRYYSLLKSNQTLA 125

Query: 168 VYLGNLVDKTYTGIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNL 227
           VYLGNL+DKTYTGIY+V I +HFYP +E           L S Y   S ADLILPISRNL
Sbjct: 126 VYLGNLIDKTYTGIYHVNISLHFYPAKEKLNSFQQKLDNLASGY--HSWADLILPISRNL 185

Query: 228 PLNDGLWFEVQNSTDVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLT 287
           PLNDGLWFEVQNS D   KEF+IPQN YRAVLEVYVSFHENDEFWYSNLPN+YI ANNL+
Sbjct: 186 PLNDGLWFEVQNSNDTELKEFKIPQNAYRAVLEVYVSFHENDEFWYSNLPNEYIAANNLS 245

Query: 288 DTPGNGPFREVLVDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPF 347
            TPGNGPFREV+V LDGE+VGAVWPFTVI+TGG+NPLLWRPI+ IGSF+LP+YDIE+TPF
Sbjct: 246 GTPGNGPFREVVVSLDGEVVGAVWPFTVIFTGGINPLLWRPITAIGSFDLPTYDIEITPF 305

Query: 348 LGNLLDGKVHNFGFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNF 407
           LG +LDGK H FGF VT+ALNVW+++ANLHLWLD  S KTE KL  H      +S   +F
Sbjct: 306 LGKILDGKSHKFGFNVTNALNVWYVDANLHLWLDKQSTKTEGKLSKHSSLPLVVSLVSDF 365

Query: 408 TGPDGTFLTKVTRSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFN 467
            G +GTFLT+ +RSVSS GWVKSS+G ITT S QD  YSNSM +G +G+ Q VNQ+I FN
Sbjct: 366 KGLNGTFLTRTSRSVSSTGWVKSSYGNITTRSIQDFYYSNSMVLGKDGNMQIVNQKIIFN 425

Query: 468 TSVYAKKESSHVYSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVIS 527
            SVY    SS+V+S+ S KTFPLY+Y++   + NG+Y  I N+ LGF EKK+ GLG   S
Sbjct: 426 DSVYINLPSSYVHSLTSHKTFPLYLYTDFLGQGNGTYLLITNVDLGFIEKKS-GLGFSNS 485

Query: 528 SLKNVQNGQGLIVVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCG 587
           SL+N+++ +G +VVK +LV SGL STQQ YRY     CY RNISSSNYTIL+D V + C 
Sbjct: 486 SLRNLRSAEGNMVVKNNLVVSGLESTQQIYRYDGGKFCYFRNISSSNYTILYDKVGSKCN 545

Query: 588 EKQ-NSPESLRFRRWPIPARRAF 610
           +K  ++ + +  R WP  AR  F
Sbjct: 546 KKSLSNLDFVLSRLWPFGARMNF 565

BLAST of CSPI04G22910 vs. ExPASy TrEMBL
Match: A0A0A0L222 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G627190 PE=4 SV=1)

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 617/622 (99.20%), Postives = 618/622 (99.36%), Query Frame = 0

Query: 1   MASSFLLLIS-LLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS 60
           MASSFLLLIS LLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS
Sbjct: 1   MASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS 60

Query: 61  KPIASPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF 120
           KPI +PKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF
Sbjct: 61  KPIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF 120

Query: 121 DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT 180
           DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT
Sbjct: 121 DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT 180

Query: 181 GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN 240
           GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN
Sbjct: 181 GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN 240

Query: 241 STDVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300
           STDV FKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL
Sbjct: 241 STDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300

Query: 301 VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF 360
           VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF
Sbjct: 301 VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF 360

Query: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT 420
           GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT
Sbjct: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT 420

Query: 421 RSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV 480
           RSVSS GWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV
Sbjct: 421 RSVSSTGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV 480

Query: 481 YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI 540
           YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI
Sbjct: 481 YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI 540

Query: 541 VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR 600
           VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR
Sbjct: 541 VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR 600

Query: 601 RWPIPARRAFLDSNLFVNNGRV 622
           RWPIPARRAFLDSNLFVNNGRV
Sbjct: 601 RWPIPARRAFLDSNLFVNNGRV 622

BLAST of CSPI04G22910 vs. ExPASy TrEMBL
Match: A0A5D3DX64 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001000 PE=4 SV=1)

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 571/620 (92.10%), Postives = 596/620 (96.13%), Query Frame = 0

Query: 2   ASSFLLLISLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKP 61
           +SSFLLLIS  LLLLLLPFHS+A+LHKTRLR+SDLFSE  V+TTADHSTPPTVFFEV+KP
Sbjct: 3   SSSFLLLIS--LLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP 62

Query: 62  IASPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 121
           I +PK+KPCSLL+LHHDFA+TYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR
Sbjct: 63  IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 122

Query: 122 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGI 181
           IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLLSKNQTLAVYLGNLVDKTYTGI
Sbjct: 123 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI 182

Query: 182 YNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 241
           YNVKI IHFYPEEEGFGGNGVYS+KLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST
Sbjct: 183 YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 242

Query: 242 DVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVD 301
           DV  KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVLVD
Sbjct: 243 DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD 302

Query: 302 LDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGF 361
           LDG+IV AVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGK H+FGF
Sbjct: 303 LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF 362

Query: 362 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRS 421
           RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTG DGTFLTKVTRS
Sbjct: 363 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS 422

Query: 422 VSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYS 481
           VSSAGWVKSSFG ITTLSNQDLSYSN M MGNNGSSQTVNQEI FN+SVYAK+ESSHVYS
Sbjct: 423 VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS 482

Query: 482 VKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVV 541
            K+LKTFPLYMYSNT D+ NGSY+SIANLTLGFNEKKTDGLG  ISSLKNVQNGQGL+VV
Sbjct: 483 SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV 542

Query: 542 KGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRW 601
           KG+LVTSGLGSTQQDYRY+DDVQCYSR+ISSSNYTILHDNVKN CGEKQNS ESLRFRRW
Sbjct: 543 KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW 602

Query: 602 PIPARRAFLDSNLFVNNGRV 622
           PIPARRA LDSNLFVNNG V
Sbjct: 603 PIPARRASLDSNLFVNNGHV 620

BLAST of CSPI04G22910 vs. ExPASy TrEMBL
Match: A0A1S3CJY5 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A OS=Cucumis melo OX=3656 GN=LOC103501812 PE=4 SV=1)

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 571/620 (92.10%), Postives = 596/620 (96.13%), Query Frame = 0

Query: 2   ASSFLLLISLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKP 61
           +SSFLLLIS  LLLLLLPFHS+A+LHKTRLR+SDLFSE  V+TTADHSTPPTVFFEV+KP
Sbjct: 3   SSSFLLLIS--LLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP 62

Query: 62  IASPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 121
           I +PK+KPCSLL+LHHDFA+TYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR
Sbjct: 63  IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 122

Query: 122 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGI 181
           IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLLSKNQTLAVYLGNLVDKTYTGI
Sbjct: 123 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI 182

Query: 182 YNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 241
           YNVKI IHFYPEEEGFGGNGVYS+KLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST
Sbjct: 183 YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 242

Query: 242 DVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVD 301
           DV  KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVLVD
Sbjct: 243 DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD 302

Query: 302 LDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGF 361
           LDG+IV AVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGK H+FGF
Sbjct: 303 LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF 362

Query: 362 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRS 421
           RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTG DGTFLTKVTRS
Sbjct: 363 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS 422

Query: 422 VSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYS 481
           VSSAGWVKSSFG ITTLSNQDLSYSN M MGNNGSSQTVNQEI FN+SVYAK+ESSHVYS
Sbjct: 423 VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS 482

Query: 482 VKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVV 541
            K+LKTFPLYMYSNT D+ NGSY+SIANLTLGFNEKKTDGLG  ISSLKNVQNGQGL+VV
Sbjct: 483 SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV 542

Query: 542 KGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRW 601
           KG+LVTSGLGSTQQDYRY+DDVQCYSR+ISSSNYTILHDNVKN CGEKQNS ESLRFRRW
Sbjct: 543 KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW 602

Query: 602 PIPARRAFLDSNLFVNNGRV 622
           PIPARRA LDSNLFVNNG V
Sbjct: 603 PIPARRASLDSNLFVNNGHV 620

BLAST of CSPI04G22910 vs. ExPASy TrEMBL
Match: A0A6J1CHN7 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A OS=Momordica charantia OX=3673 GN=LOC111011511 PE=4 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 9.0e-297
Identity = 509/622 (81.83%), Postives = 551/622 (88.59%), Query Frame = 0

Query: 1   MASSFLLLISLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSK 60
           MASS    + + LLLLLLPF+S+ANLHKTR RR DL SE   T    H+ PPT+FFEV+K
Sbjct: 1   MASSLSSPLFISLLLLLLPFYSDANLHKTRFRRPDLLSELTATADLGHTPPPTLFFEVTK 60

Query: 61  PIASPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 120
           PI +PK+KPCS+L L HDF +TYGK PVLADYTPPSHCSFTKFSKIVLEW ATCRGRQFD
Sbjct: 61  PIETPKAKPCSVLALQHDFGYTYGKSPVLADYTPPSHCSFTKFSKIVLEWMATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 180
           RIFGVWLGGVEILRSCTAEPRATGIVWTV+KDITRYSSLLSKNQTLAVYLGNLVDKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVRKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 180

Query: 181 IYNVKIRIHFYPEEEGFGGN-GVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN 240
           IY+V+I IHFYPEE+ FG N G + +K  S YDS   ADLI+PISR+LPLNDGLWFEVQ+
Sbjct: 181 IYHVRIHIHFYPEEKSFGDNGGRFLEKSASGYDSG--ADLIVPISRSLPLNDGLWFEVQS 240

Query: 241 STDVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300
           STDV  KEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL
Sbjct: 241 STDVQVKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300

Query: 301 VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF 360
           V LDG+IVGAVWPFTV+YTGGVNPLLWRPI+GIGSFNLPSYDIE+TPFLGNLLDGK HNF
Sbjct: 301 VSLDGQIVGAVWPFTVVYTGGVNPLLWRPITGIGSFNLPSYDIEITPFLGNLLDGKAHNF 360

Query: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT 420
           GFRVTHALNVW+INANLHLWLDDNS KTE KLLNHIVS    S++ NFTGPDGTF TKVT
Sbjct: 361 GFRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSPLVNSEESNFTGPDGTFSTKVT 420

Query: 421 RSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV 480
           RSVSS GWVKSS GIITT SNQD SYSNSM +G+NGSSQ VNQ+IQFN+SVYAK++SSHV
Sbjct: 421 RSVSSIGWVKSSRGIITTQSNQDFSYSNSMEIGSNGSSQVVNQKIQFNSSVYAKQQSSHV 480

Query: 481 YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI 540
           YS KSLKTFPLY+YS+TKDE NG+YQS+ANLTLGFNEKKTDG G  ISSLKN+QNGQGL+
Sbjct: 481 YSFKSLKTFPLYLYSDTKDEGNGTYQSLANLTLGFNEKKTDGFGSFISSLKNLQNGQGLM 540

Query: 541 VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR 600
           VVK HLVT GLGSTQQDYRYHDD  CY RNISSSNYTIL DNVKNTCG+K  SP SLRF 
Sbjct: 541 VVKDHLVTRGLGSTQQDYRYHDDKLCYFRNISSSNYTILFDNVKNTCGKKPESPASLRFG 600

Query: 601 RWPIPARRAFLDSNLFVNNGRV 622
           RWPIPARRAFLDS+L VN+  V
Sbjct: 601 RWPIPARRAFLDSSLQVNSVHV 620

BLAST of CSPI04G22910 vs. ExPASy TrEMBL
Match: A0A6J1FLZ6 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A-like OS=Cucurbita moschata OX=3662 GN=LOC111446428 PE=4 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.8e-289
Identity = 490/610 (80.33%), Postives = 543/610 (89.02%), Query Frame = 0

Query: 1   MASSFLLLISLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSK 60
           MASS    + + LL +LLPFHSNA+LH+T LRR DLFSE ++  T +H+TPPT+FFEV+K
Sbjct: 1   MASSLSSPLLISLLFILLPFHSNADLHRTSLRRPDLFSESELAATGNHTTPPTLFFEVTK 60

Query: 61  PIASPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 120
           PI +P+++PCSLLLL HDF +TYGK PVLADY+PPSHCSF+KFSKIVLEWKATCRGRQFD
Sbjct: 61  PIETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 180
           RIFGVWLGGVEILRSCTAEP+ATGIVWTV+KDITRY SLLSKNQTLAVYLGNL+DKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTG 180

Query: 181 IYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 240
           IY+VKI IHFYPEE+ F     YS+KL + Y  DSRADLILPISR+LPLNDGLWFEVQNS
Sbjct: 181 IYHVKIHIHFYPEEKRF----QYSEKLAAGY--DSRADLILPISRSLPLNDGLWFEVQNS 240

Query: 241 TDVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV 300
           TD+  KEFEIPQN YRAVLEVY+SFHENDEFWYSNLPNDYI+ANNL+ TPGNGPFREVLV
Sbjct: 241 TDIQVKEFEIPQNSYRAVLEVYLSFHENDEFWYSNLPNDYILANNLSQTPGNGPFREVLV 300

Query: 301 DLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFG 360
           +LD EIVGA+WPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGK H+FG
Sbjct: 301 NLDDEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKSHHFG 360

Query: 361 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTR 420
           F VTHALNVW+INANLHLWLDD S KTE KLLNHI+S  S S+DLNFTGPDGTFLTK +R
Sbjct: 361 FSVTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASR 420

Query: 421 SVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVY 480
           SVSS+GWVKSS GIITT SNQD SYSNSM MGN+G+SQ VNQEIQFN+SVYAK+ES HVY
Sbjct: 421 SVSSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESGHVY 480

Query: 481 SVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIV 540
           S KSLKTFPLYMYSNT DE NGS+QSIANLTLGFNEKK DGLG  ISSLKNVQNGQG++ 
Sbjct: 481 SFKSLKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKADGLGSFISSLKNVQNGQGMMT 540

Query: 541 VKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRR 600
           VKGHLVT GLGSTQQDYRYHDD  CYSRNISSSNYTIL+D+V + C +K  SPESLRFR+
Sbjct: 541 VKGHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESPESLRFRK 600

Query: 601 WPIPARRAFL 611
           WP  +RR FL
Sbjct: 601 WPFLSRRVFL 604

BLAST of CSPI04G22910 vs. NCBI nr
Match: XP_004146092.2 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis sativus] >KGN55069.1 hypothetical protein Csa_011983 [Cucumis sativus])

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 617/622 (99.20%), Postives = 618/622 (99.36%), Query Frame = 0

Query: 1   MASSFLLLIS-LLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS 60
           MASSFLLLIS LLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS
Sbjct: 1   MASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS 60

Query: 61  KPIASPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF 120
           KPI +PKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF
Sbjct: 61  KPIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF 120

Query: 121 DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT 180
           DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT
Sbjct: 121 DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT 180

Query: 181 GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN 240
           GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN
Sbjct: 181 GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN 240

Query: 241 STDVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300
           STDV FKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL
Sbjct: 241 STDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300

Query: 301 VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF 360
           VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF
Sbjct: 301 VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF 360

Query: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT 420
           GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT
Sbjct: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT 420

Query: 421 RSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV 480
           RSVSS GWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV
Sbjct: 421 RSVSSTGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV 480

Query: 481 YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI 540
           YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI
Sbjct: 481 YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI 540

Query: 541 VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR 600
           VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR
Sbjct: 541 VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR 600

Query: 601 RWPIPARRAFLDSNLFVNNGRV 622
           RWPIPARRAFLDSNLFVNNGRV
Sbjct: 601 RWPIPARRAFLDSNLFVNNGRV 622

BLAST of CSPI04G22910 vs. NCBI nr
Match: XP_008463736.1 (PREDICTED: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo] >KAA0066768.1 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo var. makuwa] >TYK27915.1 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo var. makuwa])

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 571/620 (92.10%), Postives = 596/620 (96.13%), Query Frame = 0

Query: 2   ASSFLLLISLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKP 61
           +SSFLLLIS  LLLLLLPFHS+A+LHKTRLR+SDLFSE  V+TTADHSTPPTVFFEV+KP
Sbjct: 3   SSSFLLLIS--LLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKP 62

Query: 62  IASPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 121
           I +PK+KPCSLL+LHHDFA+TYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR
Sbjct: 63  IVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 122

Query: 122 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGI 181
           IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLLSKNQTLAVYLGNLVDKTYTGI
Sbjct: 123 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGI 182

Query: 182 YNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 241
           YNVKI IHFYPEEEGFGGNGVYS+KLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST
Sbjct: 183 YNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNST 242

Query: 242 DVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVD 301
           DV  KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVLVD
Sbjct: 243 DVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVD 302

Query: 302 LDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGF 361
           LDG+IV AVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGK H+FGF
Sbjct: 303 LDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGF 362

Query: 362 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRS 421
           RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTG DGTFLTKVTRS
Sbjct: 363 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRS 422

Query: 422 VSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYS 481
           VSSAGWVKSSFG ITTLSNQDLSYSN M MGNNGSSQTVNQEI FN+SVYAK+ESSHVYS
Sbjct: 423 VSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYS 482

Query: 482 VKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVV 541
            K+LKTFPLYMYSNT D+ NGSY+SIANLTLGFNEKKTDGLG  ISSLKNVQNGQGL+VV
Sbjct: 483 SKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVV 542

Query: 542 KGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRW 601
           KG+LVTSGLGSTQQDYRY+DDVQCYSR+ISSSNYTILHDNVKN CGEKQNS ESLRFRRW
Sbjct: 543 KGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRW 602

Query: 602 PIPARRAFLDSNLFVNNGRV 622
           PIPARRA LDSNLFVNNG V
Sbjct: 603 PIPARRASLDSNLFVNNGHV 620

BLAST of CSPI04G22910 vs. NCBI nr
Match: XP_038899804.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Benincasa hispida])

HSP 1 Score: 1068.9 bits (2763), Expect = 1.6e-308
Identity = 530/618 (85.76%), Postives = 558/618 (90.29%), Query Frame = 0

Query: 4   SFLLLISLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKPIA 63
           S LL++ + L+ LLLPFHSNANLHK RLR SDL S+  +TTTAD++TPPTVFFEV+KPI 
Sbjct: 12  SSLLVLLISLIFLLLPFHSNANLHKIRLRHSDLLSDSGLTTTADYTTPPTVFFEVTKPIE 71

Query: 64  SPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRIF 123
           +PK+KPCSLL+L HDFAFTYGK PVLADYTPPSHCSFTKFSKIVLEW ATC+GRQFDRIF
Sbjct: 72  TPKAKPCSLLVLQHDFAFTYGKSPVLADYTPPSHCSFTKFSKIVLEWTATCQGRQFDRIF 131

Query: 124 GVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGIYN 183
           GVWLGGVEILRSCTAEPRATGIVWTVQKDITRY SLLSKNQTLAVYLGNLVDKTYTGIY+
Sbjct: 132 GVWLGGVEILRSCTAEPRATGIVWTVQKDITRYDSLLSKNQTLAVYLGNLVDKTYTGIYH 191

Query: 184 VKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTDV 243
           VKI +HFYPE +     GV S+KLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTDV
Sbjct: 192 VKIHVHFYPEAD----TGVSSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTDV 251

Query: 244 GFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVDLD 303
             KEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV LD
Sbjct: 252 QSKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVSLD 311

Query: 304 GEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGFRV 363
           GEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLG+LLDGKVHN GF V
Sbjct: 312 GEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGHLLDGKVHNCGFSV 371

Query: 364 THALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRSVS 423
           THALNVW+INANLHLWLDDNS KTE KLL+HIVS P +S DLNFTGPDGTFLTKVTRSVS
Sbjct: 372 THALNVWYINANLHLWLDDNSAKTEGKLLDHIVSPPFISVDLNFTGPDGTFLTKVTRSVS 431

Query: 424 SAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYSVK 483
           S GWVKSSFGII T SNQDLSYSNSM MGNNGSSQTV QEIQFN SVYAK+ESSHVYS K
Sbjct: 432 STGWVKSSFGIINTQSNQDLSYSNSMVMGNNGSSQTVTQEIQFNGSVYAKQESSHVYSFK 491

Query: 484 SLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVVKG 543
           SLKTFPLYMYSNT DE NG+YQSIANLTLGFNEKKTDG G   SSLKN+QNGQGL+VVKG
Sbjct: 492 SLKTFPLYMYSNTIDEGNGTYQSIANLTLGFNEKKTDGFGSFTSSLKNLQNGQGLMVVKG 551

Query: 544 HLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRWPI 603
           HLVTSGLGSTQQDYRYHDD+QCYSRNISSSNYTIL+DNVK+ CG K      L FR   I
Sbjct: 552 HLVTSGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKDFCGRKTRILRKLGFRGGSI 611

Query: 604 PARRAFLDSNLFVNNGRV 622
           PARRAFLDSNL VNN  V
Sbjct: 612 PARRAFLDSNLHVNNSHV 625

BLAST of CSPI04G22910 vs. NCBI nr
Match: XP_022140996.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Momordica charantia])

HSP 1 Score: 1028.9 bits (2659), Expect = 1.9e-296
Identity = 509/622 (81.83%), Postives = 551/622 (88.59%), Query Frame = 0

Query: 1   MASSFLLLISLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSK 60
           MASS    + + LLLLLLPF+S+ANLHKTR RR DL SE   T    H+ PPT+FFEV+K
Sbjct: 1   MASSLSSPLFISLLLLLLPFYSDANLHKTRFRRPDLLSELTATADLGHTPPPTLFFEVTK 60

Query: 61  PIASPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 120
           PI +PK+KPCS+L L HDF +TYGK PVLADYTPPSHCSFTKFSKIVLEW ATCRGRQFD
Sbjct: 61  PIETPKAKPCSVLALQHDFGYTYGKSPVLADYTPPSHCSFTKFSKIVLEWMATCRGRQFD 120

Query: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 180
           RIFGVWLGGVEILRSCTAEPRATGIVWTV+KDITRYSSLLSKNQTLAVYLGNLVDKTYTG
Sbjct: 121 RIFGVWLGGVEILRSCTAEPRATGIVWTVRKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 180

Query: 181 IYNVKIRIHFYPEEEGFGGN-GVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN 240
           IY+V+I IHFYPEE+ FG N G + +K  S YDS   ADLI+PISR+LPLNDGLWFEVQ+
Sbjct: 181 IYHVRIHIHFYPEEKSFGDNGGRFLEKSASGYDSG--ADLIVPISRSLPLNDGLWFEVQS 240

Query: 241 STDVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300
           STDV  KEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL
Sbjct: 241 STDVQVKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300

Query: 301 VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF 360
           V LDG+IVGAVWPFTV+YTGGVNPLLWRPI+GIGSFNLPSYDIE+TPFLGNLLDGK HNF
Sbjct: 301 VSLDGQIVGAVWPFTVVYTGGVNPLLWRPITGIGSFNLPSYDIEITPFLGNLLDGKAHNF 360

Query: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT 420
           GFRVTHALNVW+INANLHLWLDDNS KTE KLLNHIVS    S++ NFTGPDGTF TKVT
Sbjct: 361 GFRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSPLVNSEESNFTGPDGTFSTKVT 420

Query: 421 RSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV 480
           RSVSS GWVKSS GIITT SNQD SYSNSM +G+NGSSQ VNQ+IQFN+SVYAK++SSHV
Sbjct: 421 RSVSSIGWVKSSRGIITTQSNQDFSYSNSMEIGSNGSSQVVNQKIQFNSSVYAKQQSSHV 480

Query: 481 YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI 540
           YS KSLKTFPLY+YS+TKDE NG+YQS+ANLTLGFNEKKTDG G  ISSLKN+QNGQGL+
Sbjct: 481 YSFKSLKTFPLYLYSDTKDEGNGTYQSLANLTLGFNEKKTDGFGSFISSLKNLQNGQGLM 540

Query: 541 VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR 600
           VVK HLVT GLGSTQQDYRYHDD  CY RNISSSNYTIL DNVKNTCG+K  SP SLRF 
Sbjct: 541 VVKDHLVTRGLGSTQQDYRYHDDKLCYFRNISSSNYTILFDNVKNTCGKKPESPASLRFG 600

Query: 601 RWPIPARRAFLDSNLFVNNGRV 622
           RWPIPARRAFLDS+L VN+  V
Sbjct: 601 RWPIPARRAFLDSSLQVNSVHV 620

BLAST of CSPI04G22910 vs. NCBI nr
Match: KAG6607915.1 (hypothetical protein SDJN03_01257, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037442.1 hypothetical protein SDJN02_01069, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1006.5 bits (2601), Expect = 9.9e-290
Identity = 493/608 (81.09%), Postives = 544/608 (89.47%), Query Frame = 0

Query: 3   SSFLLLISLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKPI 62
           SS LLLIS  LL +LLPFHSNA+LH+T LRR DLFSE ++  T +H+TPPT+FFEV+KPI
Sbjct: 6   SSPLLLIS--LLFILLPFHSNADLHRTSLRRPDLFSESELAATGNHTTPPTLFFEVTKPI 65

Query: 63  ASPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRI 122
            +P+++PCSLLLL HDF +TYGK PVLADY+PPSHCSF++FS+IVLEWKATCRGRQFDRI
Sbjct: 66  ETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSRFSRIVLEWKATCRGRQFDRI 125

Query: 123 FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGIY 182
           FGVWLGGVEILRSCTAEP+ATGIVWTV+KDITRY SLLSKNQTLAVYLGNL+DKTYTGIY
Sbjct: 126 FGVWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTGIY 185

Query: 183 NVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTD 242
           +VKI IHFYPEE+ F     YS+KL + Y  DSRADLILPISR+LPLNDGLWFEVQNSTD
Sbjct: 186 HVKIHIHFYPEEKRF----QYSEKLAAGY--DSRADLILPISRSLPLNDGLWFEVQNSTD 245

Query: 243 VGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVDL 302
           +  KEFEIPQN YRAVLEVY+SFHENDEFWYSNLPNDYIIANNLT TPGNGPFREVLV+L
Sbjct: 246 IQVKEFEIPQNSYRAVLEVYLSFHENDEFWYSNLPNDYIIANNLTQTPGNGPFREVLVNL 305

Query: 303 DGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGFR 362
           D EIVGA+WPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGK H+FGF 
Sbjct: 306 DDEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKSHHFGFS 365

Query: 363 VTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRSV 422
           VTHALNVW+INANLHLWLDD S KTE KLLNHI+S  S S+DLNFTGPDGTFLTK +RSV
Sbjct: 366 VTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASRSV 425

Query: 423 SSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYSV 482
           SS+GWVKSS GIITT SNQD SYSNSM MGN+G+SQ VNQEIQFN+SVYAK+ES HVYS 
Sbjct: 426 SSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESDHVYSF 485

Query: 483 KSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVVK 542
           KSLKTFPLYMYSNT DE NGS+QSIANLTLGFNEKK DGLG  ISSLKNVQNGQG++ VK
Sbjct: 486 KSLKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKADGLGSFISSLKNVQNGQGMMTVK 545

Query: 543 GHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRWP 602
           GHLVT GLGSTQQDYRYHDD  CYSRNISSSNYTIL+D+V + C +K  SPESLRFR+WP
Sbjct: 546 GHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESPESLRFRKWP 605

Query: 603 IPARRAFL 611
             +RR FL
Sbjct: 606 FLSRRVFL 605

BLAST of CSPI04G22910 vs. TAIR 10
Match: AT3G14920.1 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A protein )

HSP 1 Score: 653.7 bits (1685), Expect = 1.5e-187
Identity = 338/604 (55.96%), Postives = 430/604 (71.19%), Query Frame = 0

Query: 1   MASSFLLLISLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTP------PTV 60
           M SS   LI    +  +    + ++LH+TR R    F  P  +     S P      PT 
Sbjct: 1   MTSSLPPLIFFFTVFFVHSLSAVSDLHETRSR----FKPPQFSPLFFSSLPQNVTKSPTR 60

Query: 61  FFEVSKPIA---SPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWK 120
           +FEV KP         +PCS  +LHHDF +TY KPPVL++YT PSHCS  +FSKIVLE+K
Sbjct: 61  YFEVQKPPVPNLPTAQQPCSYQILHHDFGYTYAKPPVLSNYTLPSHCSSREFSKIVLEFK 120

Query: 121 ATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQT--LAVY 180
           +T +GRQFDRIFGVWL GVEILRSCTAEPR  GIVW+V+KD+T+Y SLL KN+T  L+VY
Sbjct: 121 STSQGRQFDRIFGVWLDGVEILRSCTAEPRPNGIVWSVEKDVTKYHSLLVKNETQILSVY 180

Query: 181 LGNLVDKTYTGIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPL 240
           LGNL+DKTYTG+Y+V +  HFY  E        Y         S S+AD+ILPISRNLPL
Sbjct: 181 LGNLIDKTYTGVYHVDVIFHFYQSESNLQDVSGY---------SSSKADMILPISRNLPL 240

Query: 241 NDGLWFEVQNSTDVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDT 300
           NDGLWFE+ NS D  +KEFEIP+NVYRAVLEVYVSFHENDEFWY NLPNDY+ ANNL+  
Sbjct: 241 NDGLWFEIVNSNDTKYKEFEIPRNVYRAVLEVYVSFHENDEFWYGNLPNDYVTANNLS-V 300

Query: 301 PGNGPFREVLVDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLG 360
            GNGPFREV+V LDG+I GAVWPF V++TGG+NPLLWRPI+ IGSF+LPSYDIE+TPFLG
Sbjct: 301 AGNGPFREVVVSLDGDIAGAVWPFPVVFTGGINPLLWRPITAIGSFDLPSYDIEITPFLG 360

Query: 361 NLLDGKVHNFGFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTG 420
           +LLDGK H  GF VT+ALNVW+I+ANLHLWLD      E K+L+   SS  +S   +F G
Sbjct: 361 SLLDGKTHKVGFSVTNALNVWYIDANLHLWLDQEKEIVEGKVLDFSRSSLEISSVSDFKG 420

Query: 421 PDGTFLTKVTRSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTS 480
            +G F TK  RS++S G VKSS G I T +NQ+ SY N M +G +G+ Q ++Q IQ +  
Sbjct: 421 LNGNFTTKAKRSITSVGLVKSSHGDIITNANQEFSYENKMVLGKDGNLQIIDQLIQADDR 480

Query: 481 VYAKKESSHVYSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTD---GLGKVI 540
           ++AK+ S  +Y+ KS+K+FP Y+ S+T +++N +Y ++AN+++ FNE++++   GL +  
Sbjct: 481 IHAKRASREIYAAKSIKSFPFYLDSDTLEQQNNTYLAVANVSMAFNEERSESDKGLMRTF 540

Query: 541 -SSLKNVQNGQGLIVVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNT 590
            S L+N Q GQG++VVK +LV SG GSTQQ Y Y    QCY RNISS NYTIL+D V++ 
Sbjct: 541 KSKLENKQEGQGVMVVKNNLVVSGYGSTQQVYNYVGSDQCYFRNISSYNYTILYDKVESV 590

BLAST of CSPI04G22910 vs. TAIR 10
Match: AT5G05480.1 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A protein )

HSP 1 Score: 395.6 bits (1015), Expect = 7.4e-110
Identity = 223/547 (40.77%), Postives = 315/547 (57.59%), Query Frame = 0

Query: 52  PTVFFEVSKPIASPKSKP-CSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEW 111
           P  + E+ +P+ S +  P CS +L  H FA T  +PP    YTPPS C    +S +VL+ 
Sbjct: 46  PQEYEELRRPLPSDQLTPSCSHVLFRHSFANTINRPPFTTPYTPPSSCISPPWSYVVLDL 105

Query: 112 KATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQ-TLAVY 171
           +A   G Q+DRI G+WLGGVE+LR+ TAEP  +GI W V+KD++RYSSL  ++   + + 
Sbjct: 106 RAASSGDQYDRISGLWLGGVELLRTSTAEPSPSGIFWNVRKDVSRYSSLFMRSDLNVTMM 165

Query: 172 LGNLVDKTYTGIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSR----ADLILPISR 231
           L N+V+  YTGIY++ + + FY E      N   S +LG   DS  R    ADLI+P+  
Sbjct: 166 LENIVNDVYTGIYHINVTLIFY-EFNPIASNLKISNRLGFV-DSQKRDQTPADLIIPVCD 225

Query: 232 NLPLNDGLWFEVQNSTDVGFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANN 291
               N G WF ++N  +   K  +IP N  + VLE+YVSFH NDEFWYSN PN YI  NN
Sbjct: 226 G--GNRGFWFMIENPRETYSKGIQIPSNTRQIVLELYVSFHGNDEFWYSNPPNSYIRTNN 285

Query: 292 LTDTPGNGPFREVLVDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELT 351
           LT   GNG +REV V +DG  VG+  PF VI+TGG+NPL W P+  IG+FNLPSYD++LT
Sbjct: 286 LTTGRGNGAYREVFVKIDGRYVGSEVPFPVIFTGGINPLFWEPVVAIGAFNLPSYDMDLT 345

Query: 352 PFLGNLLDGKVHNFGFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPS--MSQ 411
           PFLG LLDGK H F F V   ++ W ++ANLHLWLD  S   EA   + +  SPS  M +
Sbjct: 346 PFLGLLLDGKSHEFAFGVNDGISYWLVDANLHLWLDHGSSNVEAG--SRLYDSPSRHMMR 405

Query: 412 DLNFTGPDGTFLTKVTRSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQE 471
                  DG+F  +   S    GWV+SS G +TT+        + +    NG+ + V Q 
Sbjct: 406 QEQLEQLDGSFKVEAEVSSEYDGWVRSSKGNLTTMVKSMFKVDSLVRFEKNGTYKRVEQR 465

Query: 472 IQFNTSVYAKKESSH-VYSVKSLKTFPLYMYSNT---KDEENGSYQSIANLTLGFNEKKT 531
           ++    V    ES   V  V   +T+PL + ++T      +  +Y  + N++   NE  +
Sbjct: 466 VKTERIVEVTSESGKPVNRVVHQRTYPLTVITSTLRGLTNDKDTYMLVTNVSHALNETHS 525

Query: 532 DGLGKVISSLKNVQNGQGLIVVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILH 587
           D  G+ +  + + Q+ +G + V+ H V +G  ST+Q   Y D+  CYSR I ++N  I  
Sbjct: 526 D--GEALIQVYSRQDSEGWMEVEDHNVLAGEASTKQSLSYIDEFSCYSRTIVAANGEIAQ 584