CSPI04G22020 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G22020
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptioncholine-phosphate cytidylyltransferase 2-like
LocationChr4: 20481268 .. 20486485 (+)
RNA-Seq ExpressionCSPI04G22020
SyntenyCSPI04G22020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGCCATATAAGGAGACTTCAAAAATAAAATTAAAAAAGAGAAAAGAAAAAAGAAAAAAGGAAAAAAGGAAAAAAAAGAAAAAAGAAATTAAGAGACGCACAACAAAAGCCTCAACGAAGAAAAGAATGAAAGCTAAAGAGAAGAGGAGATTGTAAGGTAATTCGAATGGCGGAGGTGAAGCCCGGCGATTCCACCACCAACACCACTACCACCACCATCACCACCACCACCGCTACCACCGCTTTCAACGGTTCTCAAAACCATTCGAATTCCGATCACCCAACGGACCGTCCGGTTCGAGTCTACGCTGATGGGATCTACGATCTCTTCCATTTTGGTCACGCTCGCTCTCTCGAGCAAGCCAAGAAATCGTACGTTTCATCTTCCATTTTCTTTTCCCTCTCCTTAATTTTTCTTCTCTTCTTGTTCTGATTCATCTATTACTCATCCCTTATGCCCTGGTTTTTACGGTTTCTGAATCTCTTCGATTTTAACTAATTTACTTCTTCTTCTTTTTTTGTTTGTTTTTTTTTTCTGATGCATTAGATTGCTTGGTTGTTCGTATTTTCATCGTTTTTATTTACTTGCCTTTACAACTCTGTGATCTTCTTTTGTATTGATCTTTTAGTGTTTAGCCTGTTTCTTTGTATCATACTTAGGATGATTATTTTCTTAAAAAATTGACTGAGACGTGCTTATTGTTCTTTTTTTTCCCTTGTAATTTCAGGTTCCCGAATACCTTTTTGCTAGTTGGATGTTGTAATGATGAGACTACCCACAAGTTTAAAGGGAAAACTGTTATGAATGAAGCTGAACGATATGAATCTCTTCGCCATTGCAAGTAGGAACTTCTGTTTTTACATGAAATTTTATTTTAGTTGGGGTTTTGGACCTTGAATCTTTGCTAATTGTTGTACATAGATGTGCTTTTTGATACGTACAACCGCCAATTAGGAGGCATGTCTGAATCCTTTAAATACAATTGTAGCTATGATGGATTAAAATTGAACTCACGATTCATCAATATCTACATGATCTTTATATATTTTCTTATGAACGTGGAATCAATCATAGTCTAATGAATTCTTGAACGTGGTTTTATTTAATTTGCTCCGTTTATTTCCCTAGGTGGGTGGATGAAGTGATTCCTGATGCACCCTGGGTGATCTCTCAAGAATTTATTGACAAGCACAAGATTGACTTTGTGGCCCATGATTCTCTTCCGTAAGTGAACCAAAAGGGTTAAGTAACTATGGGCAATGGTGTTTATTCCTTCTTTTACCTTTTATCTGTGTTTCTATCCATCTAATTAATATATAGTGGATGGTAAAGTGTACGAGGAAGTGGCCGAGTTTGTTTGTTCGTGGTGTATCTATCTGTTCATATTGAATTTATTCGTATATTCTGATTTAAATGATCTATGTCATCTCGTAACTATATCTTCTATTTTTCTGGCTTTATATAGCTATGCTGATGCTTCTGGGGCTGGTAAAGATGTTTATGAATTTGTAAGTTCAGTAGATGGGCATATTCATTTTATCAGATTGGTGCTTGGGTATCTGCCATTGCATATGATTTCATAGATGGGAACTTTTCCTTTGTAGGTTAAAAAGATTGGAAAGTTTAAGGAAACAAAAAGGACGGAAGGAATTTCAACTTCAGACATTATCATGAGGATTGTGAAAGATTATAATCAGTATGTACTGCGCAATTTGGACCGTGGTTATTCGAGGAAAGAGCTTGGAGTCAGCTTTGTGAAGGCATGATATGCATCTAGAATGATCTATTTTACTAGGATGATACTTATCTTGCTAGTCAGCGAGGGTATGTTTTATTGTTTTTGTTTCTTAACAAGCAACCCAATCTTGTTGTATACAATTATCAGGAAAAACGGCTTAGAGTAAATATGAGATTGAAGAAATTACAGGAGAAAGTTAAGGAGCATCAAGAAAAAGTGGGAGAAAAGGTACCCGTGTCTCTTTCTTTAATAATAAGTCTATTGGTACATTGTAGGGAAGTAGGGATTTTAATGTTGGCTTAATGATTTCTATGCAATAGCTGTACTTCTACCTTGGGTTTTGCATTAGAAAATATCTTTATGAGATAGCAGGCCTCTTGGATTCTTGAAGGAGTCTTTCTAGGTTTTGTTTAAAGGTGTCTTGTCTTATGTCTATTGCATCTCTGCTATGACTTGTTAATTAAACGTTAGTGTGATGAAATTTTTTTGAGATTTTCACAATGGAGAAAGCTGAATCTATTATATATTAGTTTGTCCTTCGCTGCTCATTATTGGATAAGCTTTGTATTTCTAGGTTTTGTTATAAGTATACCTAGTTGTGCTGTGTGCACTTCGGTACACTTTTGGCATCTTGCCTCCCACTTTGCAAGGCATCCTCACTCACATGATCTTAGTGAGTTTCTTATGGTTATTATGGTTATCATTATTATTCAGATATCTTTTTTTTTCATTGCTCATAAACTCTAAGCTCTTTATTTCTAATAATAGTATGATTTTTTGCTCTCTTTCCTATATACCAACCTTCTGCTTTATTCTTAAAATAATACCATGGGCCTTCTTTTATATATCCTTTTGCTTTGTTGCTCACCTTGAGCCTATAAATACAAGGCATTTATTTATTTGGATAGGAAACAATGCATTTATTCATTGCTGCTATTTTTCTTTGTGGAAGATTTTCTCAGTATGTCTTATCTCTTTAAGTCTTTATAGCTTTGGTTCCAGTACTCTCTGCCAGTTGCTTCATGTTGGTTTGTGTCGGTCATCTTTTAAAAACATCTAGCAGGTCTTGGAACTTTTAAATCATAAGCCTCTTAACTTAGAAGTGATTAATAAATCCATAACTTTAAGCTGTCTGAAAGGTACTTAACATTTTTTTCCATTTCTTACATTCATGGGATGTAAAATTGAATTTTTAGTCTAATAGGACCTTAAACTTTCAATTTTGTTTACTATGGATAATTTTATAATGTTGAATATGGTAATGACCTATTAGATATAAAATTGAAAGTCCAACTATTTTTTAGACCATAAATTGAAAGTTCACGGGACATATTAAACATAAAATAAAAGGTTCAACCTTCAGGACCTATCACATGCCATCTTGAAAGTTAAGAGACCAAACTTGAATTTAACCTTATTGTTATTATATATGTTGTACTGGAGATATCTATCATCGAGAAGAATGTTATGGGAGAATTAGAGATTGATTTTTTTTGGACCTATTAGACAAACTTAAACGTGCTTTACATTTTATTTTAGACTAAAATTACTTGAACGATGAGGACAGGTTCTTTTTACTTATTCCTAAAATTAGTGTTTTTCTGAAACTTGTTCTTAAAAGCTGTTGGTTCCAAATATTTTTGGTTTAAATTCATCTCTAGAGGAAACTATTTTGTATACATCAATTTAAAGCTTTATGTAGACTGTCTTTCTTGGCAAAGTAGAGGGGAGCAAGCTTCTTGTGTGGCTGCTGTTTCTTTTTCTTTTATCATATGGGCTTTTAGAAGAGGATTGTAGTTTGTAGTTGTACATTCATGGAGTTGAATTTTGTGCCAGCTTTCTGCCATGCGTATTTTGGCCCTAGTTTTAAAATTCAGGTTTAAGATATATGCTAATTTTAAGTTAGATGGAAATAAGTATAGTATTTCGTTTTGACAATCTTGCTTCTTTTGTGGTTCTTTTTTGAAGCATGTAAAACAAATCATGTTTTACAATTCATGATTCTGAAACTAATTGTTTCTAACTCTCTAATGCTCTGAAAGAGCACAATTCGTCTTGTAGTTTTTACTAACTCAAGCAATAAAGCTCATATGGACCCCTGGCTTTTGATATCATGACTTCGGAAACTGGGTAGTTTGTATTTAGGTTGTCCAGTATAGCAAATTGATTATCTATTTATTTTTCTAAAATATATTATATTCTACATTTCATGCAGCATGTAATTCTTTCGCATTAAGAGATTTTTCAAGCGTTTCCTGGGGTGAAGTTTTTGCTGCATAATTTCCAATTGTCCTAGCAGTTCTTTTCGCTTTTGTTGGTAGCAGTATGAGCACTGCATCCAGGTTTAAACTTTGGCCAAATGATGTTGTAACCGAAAACTTGCAACATAGAGTGCCATTTTCCATGCATCATATTTCTAATCATCGTTATTCGCTTTGAGATCATGTTTATTGTGGTTAAATCCTAATTCTGCACATTGAAGTGGAAAAAGTCCTTTTTGATTTTAATGCTAACCAGGTCTCTATCTGGCTAGTCTTGTTGGGGACCTACAATTGTATTTTATGCTTTATTTACTTGGACCTAATTATAGGCAGGATTTTTTTTTTTTTTAAATCTTTTAAAGTTGAAAATGCTTGTCTTGGATATTGCAGATTCAAACAGTTGCAAAAACTGCCGGCATGCACCGGAATGAATGGGTAGAAAATGCTGATCGATGGGTAGCTGGGTTTCTAGAGATGTTTGAAGAAGGTTGTCATAAGATGGTGAGTTAATGCATGAATTACCCCATTCCCATTTATTCATTTACTTTTGGAGTCATTAAGGCACACAAACCCATCTGTTTTGGCAAATCTTGAACTAACGTTTGACGAGTTGCATTTGAAAATCATGGGTCTGTTTAGGGCACGGCCATCAGAGATCGAATTCAAGAGCGATTGAAGGGGGCACAGCAGCTGAAATATATATCAAATGGCAAGGACAGTGATTCAGACCTTGATGAAGAATATTATTACGATGATGATAATGAAGAATATTATGATGACGACGACGATGACTTCTATGAAGAATTTGAGAAAGATGAAAAGAAAAGGTAGGGCATTTGTTTCAAGTCAAGTTTGAATACGTGTCACGTGCTTGTTGGTGAATTCTACACTATATTTTGCTACTATATGTTTAGATGATGCAGATGGTAAAAACCATAACTGCTAGTTTGTTACCAAAGCCAGAAAAAAAAAAAAGAAAGGGCACTTGGTGATAAGGTTAGGAACCATTTCTTTTGGTGACCCCTTTTTAAGATATATGTTTCTTTTTTTTGTGCATATGGATGAAAATTTCCTTTCAAGCTTTGTAAAACAGGTCGATATGAAAGCCTTTTTTTCCTTTTTCTTTTTCTTTTTGGTTTTCATGAATCATTGTAATTGAAGATTGGACTTTTGGTTTTCTTGTTTGTAGGTAATGTAAGAGTGATAAATATGGTTTTG

mRNA sequence

ACGCCATATAAGGAGACTTCAAAAATAAAATTAAAAAAGAGAAAAGAAAAAAGAAAAAAGGAAAAAAGGAAAAAAAAGAAAAAAGAAATTAAGAGACGCACAACAAAAGCCTCAACGAAGAAAAGAATGAAAGCTAAAGAGAAGAGGAGATTGTAAGGTAATTCGAATGGCGGAGGTGAAGCCCGGCGATTCCACCACCAACACCACTACCACCACCATCACCACCACCACCGCTACCACCGCTTTCAACGGTTCTCAAAACCATTCGAATTCCGATCACCCAACGGACCGTCCGGTTCGAGTCTACGCTGATGGGATCTACGATCTCTTCCATTTTGGTCACGCTCGCTCTCTCGAGCAAGCCAAGAAATCGTTCCCGAATACCTTTTTGCTAGTTGGATGTTGTAATGATGAGACTACCCACAAGTTTAAAGGGAAAACTGTTATGAATGAAGCTGAACGATATGAATCTCTTCGCCATTGCAAGTGGGTGGATGAAGTGATTCCTGATGCACCCTGGGTGATCTCTCAAGAATTTATTGACAAGCACAAGATTGACTTTGTGGCCCATGATTCTCTTCCCTATGCTGATGCTTCTGGGGCTGGTAAAGATGTTTATGAATTTGTTAAAAAGATTGGAAAGTTTAAGGAAACAAAAAGGACGGAAGGAATTTCAACTTCAGACATTATCATGAGGATTGTGAAAGATTATAATCAGTATGTACTGCGCAATTTGGACCGTGGTTATTCGAGGAAAGAGCTTGGAGTCAGCTTTGTGAAGGAAAAACGGCTTAGAGTAAATATGAGATTGAAGAAATTACAGGAGAAAGTTAAGGAGCATCAAGAAAAAGTGGGAGAAAAGATTCAAACAGTTGCAAAAACTGCCGGCATGCACCGGAATGAATGGGTAGAAAATGCTGATCGATGGGTAGCTGGGTTTCTAGAGATGTTTGAAGAAGGTTGTCATAAGATGGGCACGGCCATCAGAGATCGAATTCAAGAGCGATTGAAGGGGGCACAGCAGCTGAAATATATATCAAATGGCAAGGACAGTGATTCAGACCTTGATGAAGAATATTATTACGATGATGATAATGAAGAATATTATGATGACGACGACGATGACTTCTATGAAGAATTTGAGAAAGATGAAAAGAAAAGGTAGGGCATTTGTTTCAAGTCAAGTTTGAATACGTGTCACGTGCTTGTTGGTGAATTCTACACTATATTTTGCTACTATATGTTTAGATGATGCAGATGGTAAAAACCATAACTGCTAGTTTGTTACCAAAGCCAGAAAAAAAAAAAAGAAAGGGCACTTGGTGATAAGGTTAGGAACCATTTCTTTTGGTGACCCCTTTTTAAGATATATGTTTCTTTTTTTTGTGCATATGGATGAAAATTTCCTTTCAAGCTTTGTAAAACAGGTCGATATGAAAGCCTTTTTTTCCTTTTTCTTTTTCTTTTTGGTTTTCATGAATCATTGTAATTGAAGATTGGACTTTTGGTTTTCTTGTTTGTAGGTAATGTAAGAGTGATAAATATGGTTTTG

Coding sequence (CDS)

ATGGCGGAGGTGAAGCCCGGCGATTCCACCACCAACACCACTACCACCACCATCACCACCACCACCGCTACCACCGCTTTCAACGGTTCTCAAAACCATTCGAATTCCGATCACCCAACGGACCGTCCGGTTCGAGTCTACGCTGATGGGATCTACGATCTCTTCCATTTTGGTCACGCTCGCTCTCTCGAGCAAGCCAAGAAATCGTTCCCGAATACCTTTTTGCTAGTTGGATGTTGTAATGATGAGACTACCCACAAGTTTAAAGGGAAAACTGTTATGAATGAAGCTGAACGATATGAATCTCTTCGCCATTGCAAGTGGGTGGATGAAGTGATTCCTGATGCACCCTGGGTGATCTCTCAAGAATTTATTGACAAGCACAAGATTGACTTTGTGGCCCATGATTCTCTTCCCTATGCTGATGCTTCTGGGGCTGGTAAAGATGTTTATGAATTTGTTAAAAAGATTGGAAAGTTTAAGGAAACAAAAAGGACGGAAGGAATTTCAACTTCAGACATTATCATGAGGATTGTGAAAGATTATAATCAGTATGTACTGCGCAATTTGGACCGTGGTTATTCGAGGAAAGAGCTTGGAGTCAGCTTTGTGAAGGAAAAACGGCTTAGAGTAAATATGAGATTGAAGAAATTACAGGAGAAAGTTAAGGAGCATCAAGAAAAAGTGGGAGAAAAGATTCAAACAGTTGCAAAAACTGCCGGCATGCACCGGAATGAATGGGTAGAAAATGCTGATCGATGGGTAGCTGGGTTTCTAGAGATGTTTGAAGAAGGTTGTCATAAGATGGGCACGGCCATCAGAGATCGAATTCAAGAGCGATTGAAGGGGGCACAGCAGCTGAAATATATATCAAATGGCAAGGACAGTGATTCAGACCTTGATGAAGAATATTATTACGATGATGATAATGAAGAATATTATGATGACGACGACGATGACTTCTATGAAGAATTTGAGAAAGATGAAAAGAAAAGGTAG

Protein sequence

MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSDLDEEYYYDDDNEEYYDDDDDDFYEEFEKDEKKR*
Homology
BLAST of CSPI04G22020 vs. ExPASy Swiss-Prot
Match: Q9ZV56 (Choline-phosphate cytidylyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=CCT1 PE=1 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.3e-121
Identity = 234/328 (71.34%), Postives = 269/328 (82.01%), Query Frame = 0

Query: 7   GDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHARSLEQA 66
           GD T +  +T    + +T           S  PTDRPVRVYADGIYDLFHFGHARSLEQA
Sbjct: 6   GDRTEDGLSTAAAASGSTAV--------QSSPPTDRPVRVYADGIYDLFHFGHARSLEQA 65

Query: 67  KKSFP-NTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFI 126
           K +FP NT+LLVGCCNDETTHK+KG+TVM   ERYESLRHCKWVDEVIPDAPWV++QEF+
Sbjct: 66  KLAFPNNTYLLVGCCNDETTHKYKGRTVMTAEERYESLRHCKWVDEVIPDAPWVVNQEFL 125

Query: 127 DKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQY 186
           DKH+ID+VAHDSLPYAD+SGAGKDVYEFVKK+G+FKET+RTEGISTSDIIMRIVKDYNQY
Sbjct: 126 DKHQIDYVAHDSLPYADSSGAGKDVYEFVKKVGRFKETQRTEGISTSDIIMRIVKDYNQY 185

Query: 187 VLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRN 246
           V+RNLDRGYSR++LGVSFVKEKRLRVNMRLKKLQE+VKE QE+VGEKIQTV     M RN
Sbjct: 186 VMRNLDRGYSREDLGVSFVKEKRLRVNMRLKKLQERVKEQQERVGEKIQTVK----MLRN 245

Query: 247 EWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSD--LDEE 306
           EWVENADRWVAGFLE+FEEGCHKMGTAI D IQERL   +  + + NG+D D+D    EE
Sbjct: 246 EWVENADRWVAGFLEIFEEGCHKMGTAIVDSIQERLMRQKSAERLENGQDDDTDDQFYEE 305

Query: 307 YYYDDDNEEYYDDDDDDFYEEFEKDEKK 332
           Y+  D   +  DD+D+ FY+E E  E++
Sbjct: 306 YFDHDMGSD--DDEDEKFYDEEEVKEEE 319

BLAST of CSPI04G22020 vs. ExPASy Swiss-Prot
Match: F4JJE0 (Choline-phosphate cytidylyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=CCT2 PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 6.4e-121
Identity = 224/294 (76.19%), Postives = 250/294 (85.03%), Query Frame = 0

Query: 28  NGSQNHSNSD-HPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTFLLVGCCNDETTH 87
           NG    S  D   +DRPVRVYADGI+DLFHFGHAR++EQAKKSFPNT+LLVGCCNDE T+
Sbjct: 4   NGENKVSGGDSSSSDRPVRVYADGIFDLFHFGHARAIEQAKKSFPNTYLLVGCCNDEITN 63

Query: 88  KFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADASGA 147
           KFKGKTVM E+ERYESLRHCKWVDEVIPDAPWV++ EF+DKHKID+VAHD+LPYAD SGA
Sbjct: 64  KFKGKTVMTESERYESLRHCKWVDEVIPDAPWVLTTEFLDKHKIDYVAHDALPYADTSGA 123

Query: 148 GKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFVKE 207
           G DVYEFVK IGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSR+ELGVSF +E
Sbjct: 124 GNDVYEFVKSIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSREELGVSF-EE 183

Query: 208 KRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGC 267
           KRLRVNMRLKKLQEKVKE Q    EKIQTVAKTAGMH +EW+ENADRWVAGFLEMFEEGC
Sbjct: 184 KRLRVNMRLKKLQEKVKEQQ----EKIQTVAKTAGMHHDEWLENADRWVAGFLEMFEEGC 243

Query: 268 HKMGTAIRDRIQERL---KGAQQLKYISNGKDSDSDLDEEYYYDDDNEEYYDDD 318
           HKMGTAIRD IQ+RL   +  +  + + NG     D D+E   DD+  E+ ++D
Sbjct: 244 HKMGTAIRDGIQQRLMRQESEENRRLLQNGLTISKDNDDEQMSDDN--EFAEED 290

BLAST of CSPI04G22020 vs. ExPASy Swiss-Prot
Match: Q9Y5K3 (Choline-phosphate cytidylyltransferase B OS=Homo sapiens OX=9606 GN=PCYT1B PE=1 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 7.2e-72
Identity = 131/224 (58.48%), Postives = 168/224 (75.00%), Query Frame = 0

Query: 39  PTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAE 98
           P DRPVRVYADGI+DLFH GHAR+L QAK  FPN++LLVG C+D+ THKFKG TVMNEAE
Sbjct: 72  PADRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAE 131

Query: 99  RYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIG 158
           RYE+LRHC++VDEVI DAPW ++ EF++KHKIDFVAHD +PY  +S    DVY+ +K+ G
Sbjct: 132 RYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPY--SSAGSDDVYKHIKEAG 191

Query: 159 KFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKL 218
            F  T+RTEGISTSDII RIV+DY+ Y  RNL RGY+ KEL VSF+ EKR R   ++ K+
Sbjct: 192 MFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKRYRFQNQVDKM 251

Query: 219 QEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMF 263
           +EKVK  +E+  E +  V + +     +W E +  ++  FLE+F
Sbjct: 252 KEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELF 293

BLAST of CSPI04G22020 vs. ExPASy Swiss-Prot
Match: Q811Q9 (Choline-phosphate cytidylyltransferase B OS=Mus musculus OX=10090 GN=Pcyt1b PE=1 SV=2)

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-71
Identity = 130/224 (58.04%), Postives = 168/224 (75.00%), Query Frame = 0

Query: 39  PTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAE 98
           P DRPVRVYADGI+DLFH GHAR+L QAK  FPN++LLVG C+D+ THKFKG TVMNEAE
Sbjct: 72  PVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAE 131

Query: 99  RYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIG 158
           RYE+LRHC++VDEVI DAPW ++ EF++KHKIDFVAHD +PY  +S    DVY+ +K+ G
Sbjct: 132 RYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPY--SSAGSDDVYKHIKEAG 191

Query: 159 KFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKL 218
            F  T+RTEGISTSDII RIV+DY+ Y  RNL RGY+ KEL VSF+ EK+ R   ++ K+
Sbjct: 192 MFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYRFQNQVDKM 251

Query: 219 QEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMF 263
           +EKVK  +E+  E +  V + +     +W E +  ++  FLE+F
Sbjct: 252 KEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELF 293

BLAST of CSPI04G22020 vs. ExPASy Swiss-Prot
Match: Q9QZC4 (Choline-phosphate cytidylyltransferase B OS=Rattus norvegicus OX=10116 GN=Pcyt1b PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-71
Identity = 130/224 (58.04%), Postives = 168/224 (75.00%), Query Frame = 0

Query: 39  PTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAE 98
           P DRPVRVYADGI+DLFH GHAR+L QAK  FPN++LLVG C+D+ THKFKG TVMNEAE
Sbjct: 72  PVDRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHKFKGFTVMNEAE 131

Query: 99  RYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIG 158
           RYE+LRHC++VDEVI DAPW ++ EF++KHKIDFVAHD +PY  +S    DVY+ +K+ G
Sbjct: 132 RYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPY--SSAGSDDVYKHIKEAG 191

Query: 159 KFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKL 218
            F  T+RTEGISTSDII RIV+DY+ Y  RNL RGY+ KEL VSF+ EK+ R   ++ K+
Sbjct: 192 MFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYRFQNQVDKM 251

Query: 219 QEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMF 263
           +EKVK  +E+  E +  V + +     +W E +  ++  FLE+F
Sbjct: 252 KEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELF 293

BLAST of CSPI04G22020 vs. ExPASy TrEMBL
Match: A0A1S3CM33 (choline-phosphate cytidylyltransferase 2-like OS=Cucumis melo OX=3656 GN=LOC103502481 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.2e-176
Identity = 321/333 (96.40%), Postives = 322/333 (96.70%), Query Frame = 0

Query: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60
           MAEVKPGDSTTN     ITT T TTAFNGSQNHSNSD PTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVKPGDSTTN-----ITTDTTTTAFNGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVM EAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK
Sbjct: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240
           DYNQYVLRNLDRGYSRKELGVS+VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSYVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240

Query: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSDL 300
           GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERL GAQQLKYISNGKDSDSDL
Sbjct: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQLKYISNGKDSDSDL 300

Query: 301 DEEYYY-DDDNEEYYDDDDDDFYEEFEKDEKKR 333
           DEEYYY DDDNEEYYDDDDDDFYEEFEKDEKKR
Sbjct: 301 DEEYYYDDDDNEEYYDDDDDDFYEEFEKDEKKR 328

BLAST of CSPI04G22020 vs. ExPASy TrEMBL
Match: A0A6J1FCK6 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111442868 PE=3 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 3.7e-172
Identity = 316/334 (94.61%), Postives = 321/334 (96.11%), Query Frame = 0

Query: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60
           MAEV PG+ST  TTTTTITTTT TT  NGSQNHSNSD PTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVNPGEST--TTTTTITTTTTTTTINGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNT+LLVGCCNDETTHKFKGKTVM EAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTYLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKH ID+VAHDSLPYADASGAGKDVYEFVKKIGKFKETKRT+GISTSDIIMRIVK
Sbjct: 121 SQEFIDKHNIDYVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTDGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240
           DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240

Query: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSD-SD 300
           GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERL GAQQ KY+SNGKDSD SD
Sbjct: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLSNGKDSDYSD 300

Query: 301 LDEEYYYDD-DNEEYYDDDDDDFYEEFEKDEKKR 333
           LDEEYYYDD DNEEYYDDDDDDFYEEFEKDEKKR
Sbjct: 301 LDEEYYYDDEDNEEYYDDDDDDFYEEFEKDEKKR 332

BLAST of CSPI04G22020 vs. ExPASy TrEMBL
Match: A0A6J1IPQ3 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477078 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 4.8e-172
Identity = 316/334 (94.61%), Postives = 321/334 (96.11%), Query Frame = 0

Query: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60
           MAEV PG+ST  TTTTTITTTT TT  NGSQNHSNSD PTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVNPGEST--TTTTTITTTTTTTTVNGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNT+LLVGCCNDETTHKFKGKTVM EAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTYLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKH ID+VAHDSLPYADASGAGKDVYEFVKKIGKFKETKRT+GISTSDIIMRIVK
Sbjct: 121 SQEFIDKHNIDYVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTDGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240
           DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240

Query: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSD-SD 300
           GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERL GAQQ KY+SNGKDSD SD
Sbjct: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLSNGKDSDYSD 300

Query: 301 LDEEYYYDD-DNEEYYDDDDDDFYEEFEKDEKKR 333
           LDEEYYYDD DNEEYYDDDDDDFYEEFEKDEKKR
Sbjct: 301 LDEEYYYDDEDNEEYYDDDDDDFYEEFEKDEKKR 332

BLAST of CSPI04G22020 vs. ExPASy TrEMBL
Match: A0A6J1FJ47 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111445839 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.4e-163
Identity = 297/332 (89.46%), Postives = 304/332 (91.57%), Query Frame = 0

Query: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60
           MAEVKP DS              TT  N S NHSN D+PTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVKPADS--------------TTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNT+LLVGCCNDETTH  KGKTVMNEAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTYLLVGCCNDETTHSLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKHKIDFVAHDSLPYADA+GAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK
Sbjct: 121 SQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240
           DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240

Query: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSDL 300
           GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERL GAQQ KY+ NGKDSDSDL
Sbjct: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDL 300

Query: 301 DEEYYYDDDNEEYYDDDDDDFYEEFEKDEKKR 333
           +EEYYYDDD+EEYY   +DDFYEEFEKDEKKR
Sbjct: 301 EEEYYYDDDDEEYY---EDDFYEEFEKDEKKR 315

BLAST of CSPI04G22020 vs. ExPASy TrEMBL
Match: A0A6J1J168 (choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111480356 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 3.8e-161
Identity = 295/332 (88.86%), Postives = 302/332 (90.96%), Query Frame = 0

Query: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60
           MAEVKP DS              TT  N S NHSN D+PTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVKPADS--------------TTTINASPNHSNPDYPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNT+LLVGCCNDETTH  KGKTVMNEAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTYLLVGCCNDETTHNLKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKHKIDFVAHDSLPYADA+GAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK
Sbjct: 121 SQEFIDKHKIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240
           DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240

Query: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSDL 300
           GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERL GAQQ KY+ NGKDSDSDL
Sbjct: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLENGKDSDSDL 300

Query: 301 DEEYYYDDDNEEYYDDDDDDFYEEFEKDEKKR 333
           +EEYYYDDD+EE Y   +D FYEEFEKDEKKR
Sbjct: 301 EEEYYYDDDDEEEY--YEDGFYEEFEKDEKKR 316

BLAST of CSPI04G22020 vs. NCBI nr
Match: XP_004145998.1 (choline-phosphate cytidylyltransferase 2 isoform X1 [Cucumis sativus] >KGN55480.2 hypothetical protein Csa_012685 [Cucumis sativus])

HSP 1 Score: 656.4 bits (1692), Expect = 1.3e-184
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60
           MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK
Sbjct: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240
           DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240

Query: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSDL 300
           GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSDL
Sbjct: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSDL 300

Query: 301 DEEYYYDDDNEEYYDDDDDDFYEEFEKDEKKR 333
           DEEYYYDDDNEEYYDDDDDDFYEEFEKDEKKR
Sbjct: 301 DEEYYYDDDNEEYYDDDDDDFYEEFEKDEKKR 332

BLAST of CSPI04G22020 vs. NCBI nr
Match: XP_008464646.1 (PREDICTED: choline-phosphate cytidylyltransferase 2-like [Cucumis melo])

HSP 1 Score: 627.1 bits (1616), Expect = 8.7e-176
Identity = 321/333 (96.40%), Postives = 322/333 (96.70%), Query Frame = 0

Query: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60
           MAEVKPGDSTTN     ITT T TTAFNGSQNHSNSD PTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVKPGDSTTN-----ITTDTTTTAFNGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVM EAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK
Sbjct: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240
           DYNQYVLRNLDRGYSRKELGVS+VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSYVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240

Query: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSDL 300
           GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERL GAQQLKYISNGKDSDSDL
Sbjct: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQLKYISNGKDSDSDL 300

Query: 301 DEEYYY-DDDNEEYYDDDDDDFYEEFEKDEKKR 333
           DEEYYY DDDNEEYYDDDDDDFYEEFEKDEKKR
Sbjct: 301 DEEYYYDDDDNEEYYDDDDDDFYEEFEKDEKKR 328

BLAST of CSPI04G22020 vs. NCBI nr
Match: XP_022936193.1 (choline-phosphate cytidylyltransferase 2-like [Cucurbita moschata])

HSP 1 Score: 614.0 bits (1582), Expect = 7.6e-172
Identity = 316/334 (94.61%), Postives = 321/334 (96.11%), Query Frame = 0

Query: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60
           MAEV PG+ST  TTTTTITTTT TT  NGSQNHSNSD PTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVNPGEST--TTTTTITTTTTTTTINGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNT+LLVGCCNDETTHKFKGKTVM EAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTYLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKH ID+VAHDSLPYADASGAGKDVYEFVKKIGKFKETKRT+GISTSDIIMRIVK
Sbjct: 121 SQEFIDKHNIDYVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTDGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240
           DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240

Query: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSD-SD 300
           GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERL GAQQ KY+SNGKDSD SD
Sbjct: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLSNGKDSDYSD 300

Query: 301 LDEEYYYDD-DNEEYYDDDDDDFYEEFEKDEKKR 333
           LDEEYYYDD DNEEYYDDDDDDFYEEFEKDEKKR
Sbjct: 301 LDEEYYYDDEDNEEYYDDDDDDFYEEFEKDEKKR 332

BLAST of CSPI04G22020 vs. NCBI nr
Match: XP_022976814.1 (choline-phosphate cytidylyltransferase 2-like [Cucurbita maxima] >XP_023535856.1 choline-phosphate cytidylyltransferase 2-like [Cucurbita pepo subsp. pepo] >KAG6591810.1 Choline-phosphate cytidylyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 613.6 bits (1581), Expect = 1.0e-171
Identity = 316/334 (94.61%), Postives = 321/334 (96.11%), Query Frame = 0

Query: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60
           MAEV PG+ST  TTTTTITTTT TT  NGSQNHSNSD PTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVNPGEST--TTTTTITTTTTTTTVNGSQNHSNSDQPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNT+LLVGCCNDETTHKFKGKTVM EAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTYLLVGCCNDETTHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKH ID+VAHDSLPYADASGAGKDVYEFVKKIGKFKETKRT+GISTSDIIMRIVK
Sbjct: 121 SQEFIDKHNIDYVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTDGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240
           DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240

Query: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSD-SD 300
           GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERL GAQQ KY+SNGKDSD SD
Sbjct: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLSNGKDSDYSD 300

Query: 301 LDEEYYYDD-DNEEYYDDDDDDFYEEFEKDEKKR 333
           LDEEYYYDD DNEEYYDDDDDDFYEEFEKDEKKR
Sbjct: 301 LDEEYYYDDEDNEEYYDDDDDDFYEEFEKDEKKR 332

BLAST of CSPI04G22020 vs. NCBI nr
Match: XP_038898408.1 (choline-phosphate cytidylyltransferase 2-like [Benincasa hispida])

HSP 1 Score: 597.4 bits (1539), Expect = 7.4e-167
Identity = 307/333 (92.19%), Postives = 311/333 (93.39%), Query Frame = 0

Query: 1   MAEVKPGDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHA 60
           MAEVKPGDS           TT TTA NGSQN SNSD PTDRPVRVYADGIYDLFHFGHA
Sbjct: 1   MAEVKPGDS-----------TTTTTALNGSQNRSNSDQPTDRPVRVYADGIYDLFHFGHA 60

Query: 61  RSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVI 120
           RSLEQAKKSFPNT+LLVGCCNDE THKFKGKTVM EAERYESLRHCKWVDEVIPDAPWVI
Sbjct: 61  RSLEQAKKSFPNTYLLVGCCNDEITHKFKGKTVMTEAERYESLRHCKWVDEVIPDAPWVI 120

Query: 121 SQEFIDKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180
           SQEFIDKH IDFVAHDSLPYADA+GAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK
Sbjct: 121 SQEFIDKHNIDFVAHDSLPYADATGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVK 180

Query: 181 DYNQYVLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240
           DYNQYVLRNLDRGYSRKELGVS+VKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA
Sbjct: 181 DYNQYVLRNLDRGYSRKELGVSYVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTA 240

Query: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSDL 300
           GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERL GAQQ KY+SNGKDSDSDL
Sbjct: 241 GMHRNEWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLMGAQQQKYLSNGKDSDSDL 300

Query: 301 DEEYYYDD-DNEEYYDDDDDDFYEEFEKDEKKR 333
           DEEYYYDD DNEEYY DDDDDFYEEFEKDEKKR
Sbjct: 301 DEEYYYDDEDNEEYY-DDDDDFYEEFEKDEKKR 321

BLAST of CSPI04G22020 vs. TAIR 10
Match: AT2G32260.1 (phosphorylcholine cytidylyltransferase )

HSP 1 Score: 437.6 bits (1124), Expect = 9.1e-123
Identity = 234/328 (71.34%), Postives = 269/328 (82.01%), Query Frame = 0

Query: 7   GDSTTNTTTTTITTTTATTAFNGSQNHSNSDHPTDRPVRVYADGIYDLFHFGHARSLEQA 66
           GD T +  +T    + +T           S  PTDRPVRVYADGIYDLFHFGHARSLEQA
Sbjct: 6   GDRTEDGLSTAAAASGSTAV--------QSSPPTDRPVRVYADGIYDLFHFGHARSLEQA 65

Query: 67  KKSFP-NTFLLVGCCNDETTHKFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFI 126
           K +FP NT+LLVGCCNDETTHK+KG+TVM   ERYESLRHCKWVDEVIPDAPWV++QEF+
Sbjct: 66  KLAFPNNTYLLVGCCNDETTHKYKGRTVMTAEERYESLRHCKWVDEVIPDAPWVVNQEFL 125

Query: 127 DKHKIDFVAHDSLPYADASGAGKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQY 186
           DKH+ID+VAHDSLPYAD+SGAGKDVYEFVKK+G+FKET+RTEGISTSDIIMRIVKDYNQY
Sbjct: 126 DKHQIDYVAHDSLPYADSSGAGKDVYEFVKKVGRFKETQRTEGISTSDIIMRIVKDYNQY 185

Query: 187 VLRNLDRGYSRKELGVSFVKEKRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRN 246
           V+RNLDRGYSR++LGVSFVKEKRLRVNMRLKKLQE+VKE QE+VGEKIQTV     M RN
Sbjct: 186 VMRNLDRGYSREDLGVSFVKEKRLRVNMRLKKLQERVKEQQERVGEKIQTVK----MLRN 245

Query: 247 EWVENADRWVAGFLEMFEEGCHKMGTAIRDRIQERLKGAQQLKYISNGKDSDSD--LDEE 306
           EWVENADRWVAGFLE+FEEGCHKMGTAI D IQERL   +  + + NG+D D+D    EE
Sbjct: 246 EWVENADRWVAGFLEIFEEGCHKMGTAIVDSIQERLMRQKSAERLENGQDDDTDDQFYEE 305

Query: 307 YYYDDDNEEYYDDDDDDFYEEFEKDEKK 332
           Y+  D   +  DD+D+ FY+E E  E++
Sbjct: 306 YFDHDMGSD--DDEDEKFYDEEEVKEEE 319

BLAST of CSPI04G22020 vs. TAIR 10
Match: AT4G15130.1 (phosphorylcholine cytidylyltransferase2 )

HSP 1 Score: 435.3 bits (1118), Expect = 4.5e-122
Identity = 224/294 (76.19%), Postives = 250/294 (85.03%), Query Frame = 0

Query: 28  NGSQNHSNSD-HPTDRPVRVYADGIYDLFHFGHARSLEQAKKSFPNTFLLVGCCNDETTH 87
           NG    S  D   +DRPVRVYADGI+DLFHFGHAR++EQAKKSFPNT+LLVGCCNDE T+
Sbjct: 4   NGENKVSGGDSSSSDRPVRVYADGIFDLFHFGHARAIEQAKKSFPNTYLLVGCCNDEITN 63

Query: 88  KFKGKTVMNEAERYESLRHCKWVDEVIPDAPWVISQEFIDKHKIDFVAHDSLPYADASGA 147
           KFKGKTVM E+ERYESLRHCKWVDEVIPDAPWV++ EF+DKHKID+VAHD+LPYAD SGA
Sbjct: 64  KFKGKTVMTESERYESLRHCKWVDEVIPDAPWVLTTEFLDKHKIDYVAHDALPYADTSGA 123

Query: 148 GKDVYEFVKKIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSFVKE 207
           G DVYEFVK IGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSR+ELGVSF +E
Sbjct: 124 GNDVYEFVKSIGKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSREELGVSF-EE 183

Query: 208 KRLRVNMRLKKLQEKVKEHQEKVGEKIQTVAKTAGMHRNEWVENADRWVAGFLEMFEEGC 267
           KRLRVNMRLKKLQEKVKE Q    EKIQTVAKTAGMH +EW+ENADRWVAGFLEMFEEGC
Sbjct: 184 KRLRVNMRLKKLQEKVKEQQ----EKIQTVAKTAGMHHDEWLENADRWVAGFLEMFEEGC 243

Query: 268 HKMGTAIRDRIQERL---KGAQQLKYISNGKDSDSDLDEEYYYDDDNEEYYDDD 318
           HKMGTAIRD IQ+RL   +  +  + + NG     D D+E   DD+  E+ ++D
Sbjct: 244 HKMGTAIRDGIQQRLMRQESEENRRLLQNGLTISKDNDDEQMSDDN--EFAEED 290

BLAST of CSPI04G22020 vs. TAIR 10
Match: AT2G38670.1 (phosphorylethanolamine cytidylyltransferase 1 )

HSP 1 Score: 112.8 bits (281), Expect = 5.2e-25
Identity = 62/166 (37.35%), Postives = 95/166 (57.23%), Query Frame = 0

Query: 42  RPVRVYADGIYDLFHFGHARSLEQAKKSFPNTFLLVGCCNDETTHKFKGKTVMNEAERYE 101
           +PVRVY DG +D+ H+GH  +L QA+       L+VG  +DE     KG  V    ER  
Sbjct: 53  KPVRVYMDGCFDMMHYGHCNALRQARAL--GDQLVVGVVSDEEIIANKGPPVTPLHERMT 112

Query: 102 SLRHCKWVDEVIPDAPWVISQEFI----DKHKIDFVAHDSLPYADASGAGKDVYEFVKKI 161
            ++  KWVDEVI DAP+ I+++F+    D+++ID++ H   P     G   D Y   KK 
Sbjct: 113 MVKAVKWVDEVISDAPYAITEDFMKKLFDEYQIDYIIHGDDPCVLPDGT--DAYALAKKA 172

Query: 162 GKFKETKRTEGISTSDIIMRIVKDYNQYVLRNLDRGYSRKELGVSF 204
           G++K+ KRTEG+S++DI+ R++    +   R++   +SR  L   F
Sbjct: 173 GRYKQIKRTEGVSSTDIVGRMLLCVRE---RSISDTHSRSSLQRQF 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZV561.3e-12171.34Choline-phosphate cytidylyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=CCT1... [more]
F4JJE06.4e-12176.19Choline-phosphate cytidylyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=CCT2... [more]
Q9Y5K37.2e-7258.48Choline-phosphate cytidylyltransferase B OS=Homo sapiens OX=9606 GN=PCYT1B PE=1 ... [more]
Q811Q91.6e-7158.04Choline-phosphate cytidylyltransferase B OS=Mus musculus OX=10090 GN=Pcyt1b PE=1... [more]
Q9QZC41.6e-7158.04Choline-phosphate cytidylyltransferase B OS=Rattus norvegicus OX=10116 GN=Pcyt1b... [more]
Match NameE-valueIdentityDescription
A0A1S3CM334.2e-17696.40choline-phosphate cytidylyltransferase 2-like OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A6J1FCK63.7e-17294.61choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1IPQ34.8e-17294.61choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FJ471.4e-16389.46choline-phosphate cytidylyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1J1683.8e-16188.86choline-phosphate cytidylyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_004145998.11.3e-184100.00choline-phosphate cytidylyltransferase 2 isoform X1 [Cucumis sativus] >KGN55480.... [more]
XP_008464646.18.7e-17696.40PREDICTED: choline-phosphate cytidylyltransferase 2-like [Cucumis melo][more]
XP_022936193.17.6e-17294.61choline-phosphate cytidylyltransferase 2-like [Cucurbita moschata][more]
XP_022976814.11.0e-17194.61choline-phosphate cytidylyltransferase 2-like [Cucurbita maxima] >XP_023535856.1... [more]
XP_038898408.17.4e-16792.19choline-phosphate cytidylyltransferase 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G32260.19.1e-12371.34phosphorylcholine cytidylyltransferase [more]
AT4G15130.14.5e-12276.19phosphorylcholine cytidylyltransferase2 [more]
AT2G38670.15.2e-2537.35phosphorylethanolamine cytidylyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 208..235
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..42
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..37
NoneNo IPR availablePANTHERPTHR10739:SF52CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE 2-LIKE ISOFORM X1coord: 31..316
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 45..177
IPR004821Cytidyltransferase-like domainPFAMPF01467CTP_transf_likecoord: 47..176
e-value: 1.4E-32
score: 112.8
IPR004821Cytidyltransferase-like domainTIGRFAMTIGR00125TIGR00125coord: 45..112
e-value: 2.5E-20
score: 70.4
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 10..264
e-value: 6.4E-86
score: 289.4
IPR045049Choline-phosphate cytidylyltransferase Pcy1-likePANTHERPTHR10739CYTIDYLYLTRANSFERASEcoord: 31..316
IPR041723CTP:phosphocholine cytidylyltransferase domainCDDcd02174CCTcoord: 42..193
e-value: 7.08446E-92
score: 268.667

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G22020.1CSPI04G22020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006657 CDP-choline pathway
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0009058 biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004105 choline-phosphate cytidylyltransferase activity
molecular_function GO:0046873 metal ion transmembrane transporter activity
molecular_function GO:0031210 phosphatidylcholine binding
molecular_function GO:0003824 catalytic activity