CSPI04G20730 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G20730
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Transposon TX1 uncharacterized 149 kDa protein
Location	Chr4: 18992295 .. 18993560 (+)
RNA-Seq Expression	CSPI04G20730
Synteny	CSPI04G20730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGCTAAAAGGACTGAAAGCCATCCTAAAGAGTTGGAATAAGGAGACTTTTGGTAAGATTTTCTCCCAAAAACAGGTGCTGATTGATAAGATTAACTATCTTGACTCACTTGAAGAGTCAAGTTGTCTCAACGAGGAAAATGTGAAGGAAAGAGAAAATTGTAGAGGGGCTCTGCTTGATTTGATTGTGAAAGAGCAAAAGTTGTGGATTCAGAAGTCGAAGCTTCATTGGCTTAGAGAGGGGGAGGAGAACTCAAGCTTCTTCCACATATGGGTTTCGGCTCGTAAAAGTAAAAGTATTCTTTCTTCCTTGGTTAGTATCGAAGGGAAGACTCTTGTCACAGAGAAGGAAATTGTGGATGAGATCCTTAGTTTCTTTTCAAATTTATATGGCACAAGGATCTCCTCGCCGTTTATTTGTGACATTCTTAATTGGAGAGGCCTTAGCTTACAGGATTCGAGTTTACTTGAGGTTCCCTTTACCGAAAAAGAAATTAGAGAAGTTGTATTTGAGATGGGTTGTCTCAAGTCCCCTGGCCCTGATGGCTTGACTGGAGAGTTTTATAAAAAGTCATGGAACATTTTGAAGTCCGACCTCGTAAGGGTGTTCCAAGATTTTTTTAAAAACGGAATTATTAACAGAAGATGTAATGAGACTTATATTTATCTCATCCCCAAAAAGAAAGAGGCGGCCCGTGTCAGTGACTTCAGACCCATTAGCTTAATTACCTCCTTGTATAAAGTTATCTCCAAGGTGCTTCCAACAAGACTTAAAAAAGTTCTTCCTTCGATAATTAATGATTCTCAAATGGCTTTTGTGGAAGGAAGGCAAATCCTTGATGCTATTCTAACTGCTTCTGAGGCTGTTGACGAATGGTCTTTAAGAGGCAGAAAAGGTGTTCTTTTAAAGCTCGATTTGGAGAAAGCTTATGATAAGGTGGATTGGTCTTTTCTTGATATGGCCATGAAACTTAAAGGCTTTGGTAAGAGATGTAGGAAGTGGATATGGGGATGCTTGTCGACAACTAATTTTTCCATAATTGTCAACGGCAGGCCTAGAGGAAAGATTATTGCTAAAAGGGGCATTCGTCAAGGTGATCCTCTTGCCCCTTTTCTTTTTACGATAGTGGGAGATGCTCCAAGTTGCCTTATTCACTACTGTAATGAGAAAAGGAGTTTAAAAGGCTTTCATTTTGAGAACCTGTCAGAGGATTTAACCCATCTTCAGTATGCAGACGACACTCTTCTTTCTTCTTCCTAG

mRNA sequence

ATGGAAAAGCTAAAAGGACTGAAAGCCATCCTAAAGAGTTGGAATAAGGAGACTTTTGGTAAGATTTTCTCCCAAAAACAGGTGCTGATTGATAAGATTAACTATCTTGACTCACTTGAAGAGTCAAGTTGTCTCAACGAGGAAAATGTGAAGGAAAGAGAAAATTGTAGAGGGGCTCTGCTTGATTTGATTGTGAAAGAGCAAAAGTTGTGGATTCAGAAGTCGAAGCTTCATTGGCTTAGAGAGGGGGAGGAGAACTCAAGCTTCTTCCACATATGGGTTTCGGCTCGTAAAAGTAAAAGTATTCTTTCTTCCTTGGTTAGTATCGAAGGGAAGACTCTTGTCACAGAGAAGGAAATTGTGGATGAGATCCTTAGTTTCTTTTCAAATTTATATGGCACAAGGATCTCCTCGCCGTTTATTTGTGACATTCTTAATTGGAGAGGCCTTAGCTTACAGGATTCGAGTTTACTTGAGGTTCCCTTTACCGAAAAAGAAATTAGAGAAGTTGTATTTGAGATGGGTTGTCTCAAGTCCCCTGGCCCTGATGGCTTGACTGGAGAGTTTTATAAAAAGTCATGGAACATTTTGAAGTCCGACCTCGTAAGGGTGTTCCAAGATTTTTTTAAAAACGGAATTATTAACAGAAGATGTAATGAGACTTATATTTATCTCATCCCCAAAAAGAAAGAGGCGGCCCGTGTCAGTGACTTCAGACCCATTAGCTTAATTACCTCCTTGTATAAAGTTATCTCCAAGGTGCTTCCAACAAGACTTAAAAAAGTTCTTCCTTCGATAATTAATGATTCTCAAATGGCTTTTGTGGAAGGAAGGCAAATCCTTGATGCTATTCTAACTGCTTCTGAGGCTGTTGACGAATGGTCTTTAAGAGGCAGAAAAGGTGTTCTTTTAAAGCTCGATTTGGAGAAAGCTTATGATAAGGTGGATTGGTCTTTTCTTGATATGGCCATGAAACTTAAAGGCTTTGGTAAGAGATGTAGGAAGTGGATATGGGGATGCTTGTCGACAACTAATTTTTCCATAATTGTCAACGGCAGGCCTAGAGGAAAGATTATTGCTAAAAGGGGCATTCGTCAAGGTGATCCTCTTGCCCCTTTTCTTTTTACGATAGTGGGAGATGCTCCAAGTTGCCTTATTCACTACTGTAATGAGAAAAGGAGTTTAAAAGGCTTTCATTTTGAGAACCTGTCAGAGGATTTAACCCATCTTCAGTATGCAGACGACACTCTTCTTTCTTCTTCCTAG

Coding sequence (CDS)

ATGGAAAAGCTAAAAGGACTGAAAGCCATCCTAAAGAGTTGGAATAAGGAGACTTTTGGTAAGATTTTCTCCCAAAAACAGGTGCTGATTGATAAGATTAACTATCTTGACTCACTTGAAGAGTCAAGTTGTCTCAACGAGGAAAATGTGAAGGAAAGAGAAAATTGTAGAGGGGCTCTGCTTGATTTGATTGTGAAAGAGCAAAAGTTGTGGATTCAGAAGTCGAAGCTTCATTGGCTTAGAGAGGGGGAGGAGAACTCAAGCTTCTTCCACATATGGGTTTCGGCTCGTAAAAGTAAAAGTATTCTTTCTTCCTTGGTTAGTATCGAAGGGAAGACTCTTGTCACAGAGAAGGAAATTGTGGATGAGATCCTTAGTTTCTTTTCAAATTTATATGGCACAAGGATCTCCTCGCCGTTTATTTGTGACATTCTTAATTGGAGAGGCCTTAGCTTACAGGATTCGAGTTTACTTGAGGTTCCCTTTACCGAAAAAGAAATTAGAGAAGTTGTATTTGAGATGGGTTGTCTCAAGTCCCCTGGCCCTGATGGCTTGACTGGAGAGTTTTATAAAAAGTCATGGAACATTTTGAAGTCCGACCTCGTAAGGGTGTTCCAAGATTTTTTTAAAAACGGAATTATTAACAGAAGATGTAATGAGACTTATATTTATCTCATCCCCAAAAAGAAAGAGGCGGCCCGTGTCAGTGACTTCAGACCCATTAGCTTAATTACCTCCTTGTATAAAGTTATCTCCAAGGTGCTTCCAACAAGACTTAAAAAAGTTCTTCCTTCGATAATTAATGATTCTCAAATGGCTTTTGTGGAAGGAAGGCAAATCCTTGATGCTATTCTAACTGCTTCTGAGGCTGTTGACGAATGGTCTTTAAGAGGCAGAAAAGGTGTTCTTTTAAAGCTCGATTTGGAGAAAGCTTATGATAAGGTGGATTGGTCTTTTCTTGATATGGCCATGAAACTTAAAGGCTTTGGTAAGAGATGTAGGAAGTGGATATGGGGATGCTTGTCGACAACTAATTTTTCCATAATTGTCAACGGCAGGCCTAGAGGAAAGATTATTGCTAAAAGGGGCATTCGTCAAGGTGATCCTCTTGCCCCTTTTCTTTTTACGATAGTGGGAGATGCTCCAAGTTGCCTTATTCACTACTGTAATGAGAAAAGGAGTTTAAAAGGCTTTCATTTTGAGAACCTGTCAGAGGATTTAACCCATCTTCAGTATGCAGACGACACTCTTCTTTCTTCTTCCTAG

Protein sequence

MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGALLDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEIVDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSPGPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRPISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRKGVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIAKRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSSS*

Homology

[Close]

BLAST of CSPI04G20730 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.3e-38
Identity = 114/418 (27.27%), Postives = 202/418 (48.33%), Query Frame = 0


Query: 7   LKAILKSWNKETFGKIFSQKQVLIDKINYLD---SLEESSCLNEENVKERENCRGALLDL 66
           LK + + + K   G+  ++ + L  ++  L+   S  E   L  E ++ +E    AL ++
Sbjct: 293 LKLLCQEYTKSVSGQRNAEIEALNGEVLDLEQRLSGSEDQALQCEYLERKE----ALRNM 352

Query: 67  IVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEIVDE 126
             ++ +    +S++  L + +  S FF+     + ++  ++ L + +G  L   + I D 
Sbjct: 353 EQRQARGAFVRSRMQLLCDMDRGSRFFYALEKKKGNRKQITCLFAEDGTPLEDPEAIRDR 412

Query: 127 ILSFFSNLYGTRISSPFICDILNWRGL---SLQDSSLLEVPFTEKEIREVVFEMGCLKSP 186
             SF+ NL+     SP  C+ L W GL   S +    LE P T  E+ + +  M   KSP
Sbjct: 413 ARSFYQNLFSPDPISPDACEEL-WDGLPVVSERRKERLETPITLDELSQALRLMPHNKSP 472

Query: 187 GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 246
           G DGLT EF++  W+ L  D  RV  + FK G +   C    + L+PKK +   + ++RP
Sbjct: 473 GLDGLTIEFFQFFWDTLGPDFHRVLTEAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRP 532

Query: 247 ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 306
           +SL+++ YK+++K +  RLK VL  +I+  Q   V GR I D +    + +      G  
Sbjct: 533 VSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLLHFARRTGLS 592

Query: 307 GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 366
              L LD EKA+D+VD  +L   ++   FG +   ++    ++    + +N      +  
Sbjct: 593 LAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYLKTMYASAECLVKINWSLTAPLAF 652

Query: 367 KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLL 419
            RG+RQG PL+  L+++  +   CL+     ++ L G   +     +    YADD +L
Sbjct: 653 GRGVRQGCPLSGQLYSLAIEPFLCLL-----RKRLTGLVLKEPDMRVVLSAYADDVIL 700

[Close]

BLAST of CSPI04G20730 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 7.0e-32
Identity = 113/422 (26.78%), Postives = 189/422 (44.79%), Query Frame = 0


Query: 3   KLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGALLD 62
           K   L+A LK   +E    +    + L          EE S       KE    R  L +
Sbjct: 297 KFIALQAFLKKTEREEVNNLMGHLKQL--------EKEEHSNPKPSRRKEITKIRAELNE 356

Query: 63  LIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEIVD 122
           +  K     I KSK  +  +  +           ++ KS++SS+ +   +      EI  
Sbjct: 357 IENKRIIQQINKSKSWFFEKINKIDKPLANLTRKKRVKSLISSIRNGNDEITTDPSEIQK 416

Query: 123 EILSFFSNLYGTRISS----PFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLK 182
            +  ++  LY  +  +        +  +   LS ++  +L  P +  EI   +  +   K
Sbjct: 417 ILNEYYKKLYSHKYENLKEIDQYLEACHLPRLSQKEVEMLNRPISSSEIASTIQNLPKKK 476

Query: 183 SPGPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKK-KEAARVSD 242
           SPGPDG T EFY+     L   L+ +FQ+  K GI+     E  I LIPK  K+  R  +
Sbjct: 477 SPGPDGFTSEFYQTFKEELVPILLNLFQNIEKEGILPNTFYEANITLIPKPGKDPTRKEN 536

Query: 243 FRPISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWS-L 302
           +RPISL+    K+++K+L  R+++ +  II+  Q+ F+ G Q    I  +   +   + L
Sbjct: 537 YRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQVGFIPGSQGWFNIRKSINVIQHINKL 596

Query: 303 RGRKGVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRG 362
           + +  ++L +D EKA+D +   F+   +K  G      K I    S    +II+NG    
Sbjct: 597 KNKDHMILSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLK 656

Query: 363 KIIAKRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDT 419
               + G RQG PL+P LF IV +  +  I    E++++KG H    SE++    +ADD 
Sbjct: 657 SFPLRSGTRQGCPLSPLLFNIVMEVLAIAI---REEKAIKGIHIG--SEEIKLSLFADDM 705

[Close]

BLAST of CSPI04G20730 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.7e-30
Identity = 88/331 (26.59%), Postives = 158/331 (47.73%), Query Frame = 0


Query: 94  VSARKSKSILSSLVSIEGKTLVTEKEIVDEILSFFSNLYGTRISS----PFICDILNWRG 153
           +  ++ K+ + ++ + +G       EI   I  ++ +LY  ++ +        D      
Sbjct: 381 IKKKREKNQIDTIKNDKGDITTDPTEIQTTIREYYKHLYANKLENLEEMDTFLDTYTLPR 440

Query: 154 LSLQDSSLLEVPFTEKEIREVVFEMGCLKSPGPDGLTGEFYKKSWNILKSDLVRVFQDFF 213
           L+ ++   L  P T  EI  ++  +   KSPGPDG T EFY++    L   L+++FQ   
Sbjct: 441 LNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIE 500

Query: 214 KNGIINRRCNETYIYLIPKK-KEAARVSDFRPISLITSLYKVISKVLPTRLKKVLPSIIN 273
           K GI+     E  I LIPK  ++  +  +FRPISL+    K+++K+L  R+++ +  +I+
Sbjct: 501 KEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIH 560

Query: 274 DSQMAFVEGRQILDAILTASEAVDEWS-LRGRKGVLLKLDLEKAYDKVDWSFLDMAMKLK 333
             Q+ F+ G Q    I  +   +   +  + +  V++ +D EKA+DK+   F+   +   
Sbjct: 561 HDQVGFIPGMQGWFNIRKSINVIQHINRAKDKNHVIISIDAEKAFDKIQQPFMLKTLNKL 620

Query: 334 GFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIAKRGIRQGDPLAPFLFTIVGDAPSCLIH 393
           G      K I         +II+NG+       K G RQG PL+P LF IV +    L  
Sbjct: 621 GIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEV---LAR 680

Query: 394 YCNEKRSLKGFHFENLSEDLTHLQYADDTLL 419
              +++ +KG       E++    +ADD ++
Sbjct: 681 AIRQEKEIKGIQLG--KEEVKLSLFADDMIV 706

[Close]

BLAST of CSPI04G20730 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 2.5e-29
Identity = 92/327 (28.13%), Postives = 157/327 (48.01%), Query Frame = 0


Query: 98  KSKSILSSLVSIEGKTLVTEKEIVDEILSFFSNLYGTRISS----PFICDILNWRGLSLQ 157
           + K +++ + + +G      +EI + I SF+  LY T++ +        D      L+  
Sbjct: 392 RDKILINKIRNEKGDITTDPEEIQNTIRSFYKRLYSTKLENLDEMDKFLDRYQVPKLNQD 451

Query: 158 DSSLLEVPFTEKEIREVVFEMGCLKSPGPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGI 217
               L  P + KEI  V+  +   KSPGPDG + EFY+     L   L ++F      G 
Sbjct: 452 QVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQTFKEDLIPILHKLFHKIEVEGT 511

Query: 218 INRRCNETYIYLIPK-KKEAARVSDFRPISLITSLYKVISKVLPTRLKKVLPSIINDSQM 277
           +     E  I LIPK +K+  ++ +FRPISL+    K+++K+L  R+++ + +II+  Q+
Sbjct: 512 LPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQV 571

Query: 278 AFVEGRQILDAILTASEAVDEWS-LRGRKGVLLKLDLEKAYDKVDWSFLDMAMKLKGFGK 337
            F+ G Q    I  +   +   + L+ +  +++ LD EKA+DK+   F+   ++  G   
Sbjct: 572 GFIPGMQGWFNIRKSINVIHYINKLKDKNHMIISLDAEKAFDKIQHPFMIKVLERSGIQG 631

Query: 338 RCRKWIWGCLSTTNFSIIVNGRPRGKIIAKRGIRQGDPLAPFLFTIVGDAPSCLIHYCNE 397
                I    S    +I VNG     I  K G RQG PL+P+LF IV +    L     +
Sbjct: 632 PYLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEV---LARAIRQ 691

Query: 398 KRSLKGFHFENLSEDLTHLQYADDTLL 419
           ++ +KG       E++     ADD ++
Sbjct: 692 QKEIKGIQIG--KEEVKISLLADDMIV 713

[Close]

BLAST of CSPI04G20730 vs. ExPASy Swiss-Prot
Match: Q03274 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.1e-15
Identity = 72/246 (29.27%), Postives = 119/246 (48.37%), Query Frame = 0


Query: 179 SPGPDGLTGEFYKKSWNILKSDLVRVF-QDFFKNGIINRRCNETYIYLIPKKKEAARVSD 238
           +PG DGLT +       I ++ L R F Q     G +          LIPK  +    S+
Sbjct: 22  APGSDGLTVQ------AITRTRLPRNFVQLHLLRGHVPTPWTAMRTTLIPKDGDLENPSN 81

Query: 239 FRPISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEW--S 298
           +RPI++ ++L +++ ++L  RL+  +   ++ +Q  +      +D  L  S  +D +  S
Sbjct: 82  WRPITIASALQRLLHRILAKRLEAAVE--LHPAQKGYAR----IDGTLVNSLLLDTYISS 141

Query: 299 LRGRKGV--LLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVN-G 358
            R ++    ++ LD+ KA+D V  S +  A++  G  +    +I G LS +  +I V  G
Sbjct: 142 RREQRKTYNVVSLDVRKAFDTVSHSSICRALQRLGIDEGTSNYITGSLSDSTTTIRVGPG 201

Query: 359 RPRGKIIAKRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQY 418
               KI  +RG++QGDPL+PFLF  V D   C +      +S  G       E +  L +
Sbjct: 202 SQTRKICIRRGVKQGDPLSPFLFNAVLDELLCSL------QSTPGIGGTIGEEKIPVLAF 249

[Close]

BLAST of CSPI04G20730 vs. ExPASy TrEMBL
Match: A0A438IK87 (Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX2_766 PE=4 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 7.6e-106
Identity = 191/420 (45.48%), Postives = 285/420 (67.86%), Query Frame = 0


Query: 1   MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
           M KLK +K+ LK WN  TFG +  +K++++  ++ +D +E+   LN + V ER   R  L
Sbjct: 30  MRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLKRREL 89

Query: 61  LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
            D+++KE+  W QKS++ W++EG+ NS FFH   + RKS+  + SL+S  G+TL   ++I
Sbjct: 90  EDVLLKEEVQWRQKSRIKWIKEGDCNSKFFHRVATGRKSRKFIKSLISERGETLNNIEDI 149

Query: 121 VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
            +EI++FF NLY   +   +  + ++W  +S +    L+ PFTE+E+R  VF++   K+P
Sbjct: 150 SEEIVNFFGNLYSKPVGESWRVEGIDWVPISGESGVWLDRPFTEEEVRRAVFQLNKEKAP 209

Query: 181 GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
           GPDG T   Y++ W+++K DL+RVF +F  NG+IN+  N T+I L+PKK ++ ++SD+RP
Sbjct: 210 GPDGFTIAVYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSVKISDYRP 269

Query: 241 ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
           ISL+TSLYK+I+KVL  RL+KVL   I+DSQ AFVEGR ILDA+L A+E VDE    G +
Sbjct: 270 ISLVTSLYKIIAKVLSGRLRKVLHETISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEE 329

Query: 301 GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
           G++ K+D EKAYD VDW FLD  ++ KGF ++ R WI GCLS+++F+I+VNG  +G + A
Sbjct: 330 GIVFKIDFEKAYDHVDWGFLDHVLQRKGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKA 389

Query: 361 KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
            RG+RQGDPL+PFLFT+V D  S ++    E    +GF        ++ LQ+ADDT+  S
Sbjct: 390 SRGLRQGDPLSPFLFTLVADVLSRMLFRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFS 449

[Close]

BLAST of CSPI04G20730 vs. ExPASy TrEMBL
Match: A0A438FWU5 (LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=LORF2_70 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 1.3e-105
Identity = 190/420 (45.24%), Postives = 285/420 (67.86%), Query Frame = 0


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            M KLK +K+ LK WN  TFG +  +K++++  ++ +D +E+   LN + V ER   R  L
Sbjct: 1010 MRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLKRREL 1069

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             D+++KE+  W QKS++ W++EG+ NS FFH   + R+S+  + SL+S  G+TL   ++I
Sbjct: 1070 EDVLLKEEVQWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKFIKSLISERGETLNNIEDI 1129

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EI++FF NLY   +   +  + ++W  +S +    L+ PFTE+E+R  VF++   K+P
Sbjct: 1130 SEEIVNFFGNLYSKPVGESWRVEGIDWVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAP 1189

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   Y++ W+++K DL+RVF +F  NG+IN+  N T+I L+PKK ++ ++SD+RP
Sbjct: 1190 GPDGFTIAVYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSVKISDYRP 1249

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISL+TSLYK+I+KVL  RL+KVL   I+DSQ AFVEGR ILDA+L A+E VDE    G +
Sbjct: 1250 ISLVTSLYKIIAKVLSGRLRKVLHETISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEE 1309

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            G++ K+D EKAYD VDW FLD  ++ KGF ++ R WI GCLS+++F+I+VNG  +G + A
Sbjct: 1310 GIVFKIDFEKAYDHVDWGFLDHVLQRKGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKA 1369

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFT+V D  S ++    E    +GF        ++ LQ+ADDT+  S
Sbjct: 1370 SRGLRQGDPLSPFLFTLVADVLSRMLFRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFS 1429

[Close]

BLAST of CSPI04G20730 vs. ExPASy TrEMBL
Match: A5BV95 (Reverse transcriptase domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_026478 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 1.3e-105
Identity = 190/420 (45.24%), Postives = 285/420 (67.86%), Query Frame = 0


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            M KLK +K+ LK WN  TFG +  +K++++  ++ +D +E+   LN + V ER   R  L
Sbjct: 897  MRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLKRREL 956

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             D+++KE+  W QKS++ W++EG+ NS FFH   + R+S+  + SL+S  G+TL   ++I
Sbjct: 957  EDVLLKEEVQWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKFIKSLISERGETLNNIEDI 1016

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EI++FF NLY   +   +  + ++W  +S +    L+ PFTE+E+R  VF++   K+P
Sbjct: 1017 SEEIVNFFGNLYSKPVGESWRXEGIDWVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAP 1076

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   Y++ W+++K DL+RVF +F  NG+IN+  N T+I L+PKK ++ ++SD+RP
Sbjct: 1077 GPDGFTIAVYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSVKISDYRP 1136

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISL+TSLYK+I+KVL  RL+KVL   I+DSQ AFVEGR ILDA+L A+E VDE    G +
Sbjct: 1137 ISLVTSLYKIIAKVLSGRLRKVLHETISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEE 1196

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            G++ K+D EKAYD VDW FLD  ++ KGF ++ R WI GCLS+++F+I+VNG  +G + A
Sbjct: 1197 GIVFKIDFEKAYDHVDWGFLDHVLQRKGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKA 1256

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFT+V D  S ++    E    +GF        ++ LQ+ADDT+  S
Sbjct: 1257 SRGLRQGDPLSPFLFTLVADVLSRMLFRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFS 1316

[Close]

BLAST of CSPI04G20730 vs. ExPASy TrEMBL
Match: A0A2P6QQZ3 (Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0393411 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 1.7e-105
Identity = 192/417 (46.04%), Postives = 280/417 (67.15%), Query Frame = 0


Query: 1   MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
           M KLK +K  LK W++ETFG I  +K+V+  +IN LD  E S+ +     +ERE  RG L
Sbjct: 30  MRKLKNVKGKLKIWSRETFGDIGKEKKVVEARINELDEEERSAGICVVKKREREVLRGRL 89

Query: 61  LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
            +L ++E+  W Q++KL W +EG+ N+ FFH  V+ R+ ++++  L    G  +  E  I
Sbjct: 90  EELALREEIFWRQRAKLKWAKEGDNNTRFFHKLVNGRRKRNVIEKLELSNGIVVEEEDLI 149

Query: 121 VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
            +EI+ F+ NLY ++    F  + L+W  +S++ +  LE PF E+EI+  VFE   +KSP
Sbjct: 150 EEEIIRFYKNLYSSKEEVSFGIEGLHWNPISVEKARWLETPFEEEEIKRAVFECDTVKSP 209

Query: 181 GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
           GPDG +    +++W ++K +++ V  +F  NG++N+  NETYI LIPKK  + +V D+RP
Sbjct: 210 GPDGFSFAVLQRNWEVVKREVLDVMAEFHTNGVVNKVTNETYICLIPKKANSLKVGDYRP 269

Query: 241 ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
           ISLITSLYK+I+KVL  RL++VL   I+ +Q AF++GRQILDA+L A+E VDE   + ++
Sbjct: 270 ISLITSLYKIIAKVLAWRLREVLSDTISGAQGAFIKGRQILDAVLVANEVVDETRKKKKE 329

Query: 301 GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
           G++ K+D EKAYD V+W+FLD AM+ KGFG R RKWI GCL + NFSI +NGRPRGK  A
Sbjct: 330 GLVFKIDFEKAYDHVEWNFLDYAMESKGFGDRWRKWIGGCLRSANFSIQINGRPRGKFGA 389

Query: 361 KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTL 418
            RG+RQGDPL+ FLFT+V D    L+    + R ++G        ++THLQ+ADDT+
Sbjct: 390 SRGLRQGDPLSSFLFTLVVDVLDRLMERARDCRLIEGLVVGREEVEITHLQFADDTI 446

[Close]

BLAST of CSPI04G20730 vs. ExPASy TrEMBL
Match: A0A5E4GN72 (PREDICTED: RNA-directed DNA polymerase (Fragment) OS=Prunus dulcis OX=3755 GN=ALMOND_2B014918 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 2.2e-105
Identity = 193/415 (46.51%), Postives = 275/415 (66.27%), Query Frame = 0


Query: 3   KLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGALLD 62
           +L+ +K  +K WNKE FG + S K+    +I  LD +E    L+    KERE+    + D
Sbjct: 212 RLRTIKQKIKVWNKEVFGDLVSAKKEAEARIAALDLMEGQGGLDNTLRKEREDLYFMVSD 271

Query: 63  LIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEIVD 122
           L+ KE+  W Q+ K+ W R+G+ N+ FFH   S R+ ++ +  L    G  +V+E EI  
Sbjct: 272 LVHKEEVKWRQRGKIQWARDGDSNTKFFHRIASGRRKRNFIQKLEVAGGGVVVSEGEIEL 331

Query: 123 EILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSPGP 182
           EI++FF NLY + + + +  + LNW  +S++++  L+ PF E+E++  VF+ G  KSPGP
Sbjct: 332 EIINFFKNLYSSNVEAGWCLEGLNWNAISVEEAEWLDRPFEEEEVKRAVFDCGIDKSPGP 391

Query: 183 DGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRPIS 242
           DG +   ++  W+I+K DL++V  DFF  GIIN   NET+I LIPKKKE+ +VSDFRPIS
Sbjct: 392 DGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINAITNETFICLIPKKKESVKVSDFRPIS 451

Query: 243 LITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRKGV 302
           L+TSLYK++SKVL +RL++VL S I+  Q AFV+GRQILDA L A+E V+E     + G+
Sbjct: 452 LVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGM 511

Query: 303 LLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIAKR 362
           + K+DLEKAYD V+W F+D  +  KGFG R R WI GCL T NFS+++NGRPRGK  A R
Sbjct: 512 VFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSWIRGCLETANFSVMINGRPRGKFRASR 571

Query: 363 GIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTL 418
           G+RQGDPL+PFLFT+V D  S ++    +     G    N   +++HLQ+ADDT+
Sbjct: 572 GLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFHGLSPGNGMVEISHLQFADDTI 626

[Close]

BLAST of CSPI04G20730 vs. NCBI nr
Match: RVW97045.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 394.0 bits (1011), Expect = 1.6e-105
Identity = 191/420 (45.48%), Postives = 285/420 (67.86%), Query Frame = 0


Query: 1   MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
           M KLK +K+ LK WN  TFG +  +K++++  ++ +D +E+   LN + V ER   R  L
Sbjct: 30  MRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLKRREL 89

Query: 61  LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
            D+++KE+  W QKS++ W++EG+ NS FFH   + RKS+  + SL+S  G+TL   ++I
Sbjct: 90  EDVLLKEEVQWRQKSRIKWIKEGDCNSKFFHRVATGRKSRKFIKSLISERGETLNNIEDI 149

Query: 121 VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
            +EI++FF NLY   +   +  + ++W  +S +    L+ PFTE+E+R  VF++   K+P
Sbjct: 150 SEEIVNFFGNLYSKPVGESWRVEGIDWVPISGESGVWLDRPFTEEEVRRAVFQLNKEKAP 209

Query: 181 GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
           GPDG T   Y++ W+++K DL+RVF +F  NG+IN+  N T+I L+PKK ++ ++SD+RP
Sbjct: 210 GPDGFTIAVYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSVKISDYRP 269

Query: 241 ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
           ISL+TSLYK+I+KVL  RL+KVL   I+DSQ AFVEGR ILDA+L A+E VDE    G +
Sbjct: 270 ISLVTSLYKIIAKVLSGRLRKVLHETISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEE 329

Query: 301 GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
           G++ K+D EKAYD VDW FLD  ++ KGF ++ R WI GCLS+++F+I+VNG  +G + A
Sbjct: 330 GIVFKIDFEKAYDHVDWGFLDHVLQRKGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKA 389

Query: 361 KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
            RG+RQGDPL+PFLFT+V D  S ++    E    +GF        ++ LQ+ADDT+  S
Sbjct: 390 SRGLRQGDPLSPFLFTLVADVLSRMLFRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFS 449

[Close]

BLAST of CSPI04G20730 vs. NCBI nr
Match: RVW64408.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 393.3 bits (1009), Expect = 2.7e-105
Identity = 190/420 (45.24%), Postives = 285/420 (67.86%), Query Frame = 0


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            M KLK +K+ LK WN  TFG +  +K++++  ++ +D +E+   LN + V ER   R  L
Sbjct: 1010 MRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLKRREL 1069

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             D+++KE+  W QKS++ W++EG+ NS FFH   + R+S+  + SL+S  G+TL   ++I
Sbjct: 1070 EDVLLKEEVQWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKFIKSLISERGETLNNIEDI 1129

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EI++FF NLY   +   +  + ++W  +S +    L+ PFTE+E+R  VF++   K+P
Sbjct: 1130 SEEIVNFFGNLYSKPVGESWRVEGIDWVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAP 1189

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   Y++ W+++K DL+RVF +F  NG+IN+  N T+I L+PKK ++ ++SD+RP
Sbjct: 1190 GPDGFTIAVYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSVKISDYRP 1249

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISL+TSLYK+I+KVL  RL+KVL   I+DSQ AFVEGR ILDA+L A+E VDE    G +
Sbjct: 1250 ISLVTSLYKIIAKVLSGRLRKVLHETISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEE 1309

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            G++ K+D EKAYD VDW FLD  ++ KGF ++ R WI GCLS+++F+I+VNG  +G + A
Sbjct: 1310 GIVFKIDFEKAYDHVDWGFLDHVLQRKGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKA 1369

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFT+V D  S ++    E    +GF        ++ LQ+ADDT+  S
Sbjct: 1370 SRGLRQGDPLSPFLFTLVADVLSRMLFRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFS 1429

[Close]

BLAST of CSPI04G20730 vs. NCBI nr
Match: CAN75040.1 (hypothetical protein VITISV_026478 [Vitis vinifera])

HSP 1 Score: 393.3 bits (1009), Expect = 2.7e-105
Identity = 190/420 (45.24%), Postives = 285/420 (67.86%), Query Frame = 0


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            M KLK +K+ LK WN  TFG +  +K++++  ++ +D +E+   LN + V ER   R  L
Sbjct: 897  MRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLKRREL 956

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             D+++KE+  W QKS++ W++EG+ NS FFH   + R+S+  + SL+S  G+TL   ++I
Sbjct: 957  EDVLLKEEVQWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKFIKSLISERGETLNNIEDI 1016

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EI++FF NLY   +   +  + ++W  +S +    L+ PFTE+E+R  VF++   K+P
Sbjct: 1017 SEEIVNFFGNLYSKPVGESWRXEGIDWVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAP 1076

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   Y++ W+++K DL+RVF +F  NG+IN+  N T+I L+PKK ++ ++SD+RP
Sbjct: 1077 GPDGFTIAVYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSVKISDYRP 1136

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISL+TSLYK+I+KVL  RL+KVL   I+DSQ AFVEGR ILDA+L A+E VDE    G +
Sbjct: 1137 ISLVTSLYKIIAKVLSGRLRKVLHETISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEE 1196

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            G++ K+D EKAYD VDW FLD  ++ KGF ++ R WI GCLS+++F+I+VNG  +G + A
Sbjct: 1197 GIVFKIDFEKAYDHVDWGFLDHVLQRKGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKA 1256

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFT+V D  S ++    E    +GF        ++ LQ+ADDT+  S
Sbjct: 1257 SRGLRQGDPLSPFLFTLVADVLSRMLFRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFS 1316

[Close]

BLAST of CSPI04G20730 vs. NCBI nr
Match: PRQ36601.1 (putative RNA-directed DNA polymerase [Rosa chinensis])

HSP 1 Score: 392.9 bits (1008), Expect = 3.5e-105
Identity = 192/417 (46.04%), Postives = 280/417 (67.15%), Query Frame = 0


Query: 1   MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
           M KLK +K  LK W++ETFG I  +K+V+  +IN LD  E S+ +     +ERE  RG L
Sbjct: 30  MRKLKNVKGKLKIWSRETFGDIGKEKKVVEARINELDEEERSAGICVVKKREREVLRGRL 89

Query: 61  LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
            +L ++E+  W Q++KL W +EG+ N+ FFH  V+ R+ ++++  L    G  +  E  I
Sbjct: 90  EELALREEIFWRQRAKLKWAKEGDNNTRFFHKLVNGRRKRNVIEKLELSNGIVVEEEDLI 149

Query: 121 VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
            +EI+ F+ NLY ++    F  + L+W  +S++ +  LE PF E+EI+  VFE   +KSP
Sbjct: 150 EEEIIRFYKNLYSSKEEVSFGIEGLHWNPISVEKARWLETPFEEEEIKRAVFECDTVKSP 209

Query: 181 GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
           GPDG +    +++W ++K +++ V  +F  NG++N+  NETYI LIPKK  + +V D+RP
Sbjct: 210 GPDGFSFAVLQRNWEVVKREVLDVMAEFHTNGVVNKVTNETYICLIPKKANSLKVGDYRP 269

Query: 241 ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
           ISLITSLYK+I+KVL  RL++VL   I+ +Q AF++GRQILDA+L A+E VDE   + ++
Sbjct: 270 ISLITSLYKIIAKVLAWRLREVLSDTISGAQGAFIKGRQILDAVLVANEVVDETRKKKKE 329

Query: 301 GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
           G++ K+D EKAYD V+W+FLD AM+ KGFG R RKWI GCL + NFSI +NGRPRGK  A
Sbjct: 330 GLVFKIDFEKAYDHVEWNFLDYAMESKGFGDRWRKWIGGCLRSANFSIQINGRPRGKFGA 389

Query: 361 KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTL 418
            RG+RQGDPL+ FLFT+V D    L+    + R ++G        ++THLQ+ADDT+
Sbjct: 390 SRGLRQGDPLSSFLFTLVVDVLDRLMERARDCRLIEGLVVGREEVEITHLQFADDTI 446

[Close]

BLAST of CSPI04G20730 vs. NCBI nr
Match: BBN69746.1 (VIRB2-interacting protein 2 [Prunus dulcis])

HSP 1 Score: 392.5 bits (1007), Expect = 4.6e-105
Identity = 193/415 (46.51%), Postives = 275/415 (66.27%), Query Frame = 0


Query: 3   KLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGALLD 62
           +L+ +K  +K WNKE FG + S K+    +I  LD +E    L+    KERE+    + D
Sbjct: 32  RLRTIKQKIKVWNKEVFGDLVSAKKEAEARIAALDLMEGQGGLDNTLRKEREDLYFMVSD 91

Query: 63  LIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEIVD 122
           L+ KE+  W Q+ K+ W R+G+ N+ FFH   S R+ ++ +  L    G  +V+E EI  
Sbjct: 92  LVHKEEVKWRQRGKIQWARDGDSNTKFFHRIASGRRKRNFIQKLEVAGGGVVVSEGEIEL 151

Query: 123 EILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSPGP 182
           EI++FF NLY + + + +  + LNW  +S++++  L+ PF E+E++  VF+ G  KSPGP
Sbjct: 152 EIINFFKNLYSSNVEAGWCLEGLNWNAISVEEAEWLDRPFEEEEVKRAVFDCGIDKSPGP 211

Query: 183 DGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRPIS 242
           DG +   ++  W+I+K DL++V  DFF  GIIN   NET+I LIPKKKE+ +VSDFRPIS
Sbjct: 212 DGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINAITNETFICLIPKKKESVKVSDFRPIS 271

Query: 243 LITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRKGV 302
           L+TSLYK++SKVL +RL++VL S I+  Q AFV+GRQILDA L A+E V+E     + G+
Sbjct: 272 LVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGM 331

Query: 303 LLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIAKR 362
           + K+DLEKAYD V+W F+D  +  KGFG R R WI GCL T NFS+++NGRPRGK  A R
Sbjct: 332 VFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSWIRGCLETANFSVMINGRPRGKFRASR 391

Query: 363 GIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTL 418
           G+RQGDPL+PFLFT+V D  S ++    +     G    N   +++HLQ+ADDT+
Sbjct: 392 GLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFHGLSPGNGMVEISHLQFADDTI 446

[Close]

BLAST of CSPI04G20730 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 2.2e-25
Identity = 78/262 (29.77%), Postives = 135/262 (51.53%), Query Frame = 0


Query: 2   EKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSL-----EESSCLNEENVKERENC 61
           E LK  K   K  N++ FG I  + +  +D +  + S       +S    E   +++ N 
Sbjct: 367 EHLKAAKKCCKLLNRQGFGNIQHKTKEALDSLESIQSQLLTNPSDSLFRVEHVARKKWNF 426

Query: 62  RGALLDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVT 121
             A L      +  + QKS++ WL++G+ N+ FFH  + A ++K+++  L   +   +  
Sbjct: 427 FAAAL------ESFYRQKSRIKWLQDGDANTRFFHKVILANQAKNLIKFLRMDDDVRVEN 486

Query: 122 EKEIVDEILSFFSNLYG------TRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREV 181
             ++ + I++++++L G      T  S   I DI  +R      S L  +P ++KEI   
Sbjct: 487 VTQVKEMIVAYYTHLLGSDSDILTPDSVQRIKDIHPFRCNDTLASRLSALP-SDKEITAA 546

Query: 182 VFEMGCLKSPGPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKK 241
           VF M   K+PGPD  T EF+ +SW ++K   +   ++FF+ G + +R N T I LIPK  
Sbjct: 547 VFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVKEFFRTGHLLKRFNATAITLIPKVT 606

Query: 242 EAARVSDFRPISLITSLYKVIS 253
              ++S FRP+S  T +YK+I+
Sbjct: 607 GVDQLSMFRPVSCCTVVYKIIT 621

[Close]

BLAST of CSPI04G20730 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 65.9 bits (159), Expect = 9.2e-11
Identity = 29/68 (42.65%), Postives = 40/68 (58.82%), Query Frame = 0


Query: 349 IVNGRPRGKIIAKRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLT 408
           I+NG P+G +   RG+RQGDPL+P+LF +  +  S L     E+  L G    N S  + 
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 409 HLQYADDT 417
           HL +ADDT
Sbjct: 73  HLLFADDT 80

[Close]

BLAST of CSPI04G20730 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 59.3 bits (142), Expect = 8.6e-09
Identity = 33/76 (43.42%), Postives = 48/76 (63.16%), Query Frame = 0


Query: 258 RLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRKGV----LLKLDLEKAYD 317
           RLK ++ ++I  +Q +F+ GR   D I+   EAV   S+R +KGV    LLKLDLEKAYD
Sbjct: 4   RLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVH--SMRRKKGVKGWMLLKLDLEKAYD 63

Query: 318 KVDWSFLDMAMKLKGF 330
           ++ W +L+  +   GF
Sbjct: 64  RIRWDYLEDTLISAGF 77

The following BLAST results are available for this feature:

BLAST of CSPI04G20730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14381	2.3e-38	27.27	Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV...	[more]
P08548	7.0e-32	26.78	LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1	[more]
O00370	1.7e-30	26.59	LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1	[more]
P11369	2.5e-29	28.13	LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE...	[more]
Q03274	2.1e-15	29.27	Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra...	[more]

BLAST of CSPI04G20730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A438IK87	7.6e-106	45.48	Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX...	[more]
A0A438FWU5	1.3e-105	45.24	LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=LORF...	[more]
A5BV95	1.3e-105	45.24	Reverse transcriptase domain-containing protein OS=Vitis vinifera OX=29760 GN=VI...	[more]
A0A2P6QQZ3	1.7e-105	46.04	Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4...	[more]
A0A5E4GN72	2.2e-105	46.51	PREDICTED: RNA-directed DNA polymerase (Fragment) OS=Prunus dulcis OX=3755 GN=AL...	[more]

BLAST of CSPI04G20730 vs. NCBI nr


Match Name	E-value	Identity	Description
RVW97045.1	1.6e-105	45.48	Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera]	[more]
RVW64408.1	2.7e-105	45.24	LINE-1 retrotransposable element ORF2 protein [Vitis vinifera]	[more]
CAN75040.1	2.7e-105	45.24	hypothetical protein VITISV_026478 [Vitis vinifera]	[more]
PRQ36601.1	3.5e-105	46.04	putative RNA-directed DNA polymerase [Rosa chinensis]	[more]
BBN69746.1	4.6e-105	46.51	VIRB2-interacting protein 2 [Prunus dulcis]	[more]

BLAST of CSPI04G20730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G43760.1	2.2e-25	29.77	DNAse I-like superfamily protein	[more]
ATMG01250.1	9.2e-11	42.65	RNA-directed DNA polymerase (reverse transcriptase)	[more]
AT4G20520.1	8.6e-09	43.42	RNA binding;RNA-directed DNA polymerases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 226..420 e-value: 1.5E-37 score: 129.3
IPR000477	Reverse transcriptase domain	PROSITE	PS50878	RT_POL	coord: 207..421 score: 15.804279
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 8..396
None	No IPR available	PANTHER	PTHR33116:SF38	OS01G0158850 PROTEIN	coord: 8..396
None	No IPR available	CDD	cd01650	RT_nLTR_like	coord: 220..418 e-value: 1.62326E-42 score: 147.053
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 178..420

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G20730.1	CSPI04G20730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
biological_process	GO:0048583	regulation of response to stimulus
molecular_function	GO:0016740	transferase activity