CSPI04G18850 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G18850
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionAmylomaltase
LocationChr4: 16363006 .. 16366570 (+)
RNA-Seq ExpressionCSPI04G18850
SyntenyCSPI04G18850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAATTTGTATAAATCCCCATGAAAATCATCGAAGGTAAGATATTTTACAACTTAGATCATTCCATGTGCTTTTAAATTTTGATGCTTTCGTTCTCCTCCACATGGGTCCGAAGATTTTCCGGGAAAATTTCGTGGGGAAAAGTTTCTCAGAACTGTCCAAATGAATTTGTATAACAAGAACAGAAAGATTTCAATTCTACGGTCACTATTCACTTTTTCATTGAACGCCAAAATACAACTCGTTCGAACCTCTCTCTACTACTACAAGACATTCATCACCAAAATATCAAGCAACCCCATCAATCCCTCTCTTACTTTATATCTTCGAGAACGTGGTGATTACTGATTTCCCACTTCCCTACTATATTCTACACCAGCAAGGGAGATTCTCCTTACTGCATTTTCCAACCGAACGAATTCATCTCTTCAATTTCGTTCTTCCTATTTGGTGAGTGAAATATTTTTTATGGGTGTAGCTTAATTTCTTTGAAATTCGATTGTGAAGTTGTATCTCGTTTGGTTTGCCTAGTTCATGTTTCCTTCTTGTTTTGAGTTTCAATCTGGAGAATTAAGCAATTTGCATCTAGCTGAAATGTGAGTGCATTTATTTGTGTTTTGCTGTGTAGAAAACTTGTTCTGGGTCTGTTTCAATTCTGAATAAGATGGTAAATTTGGGATTGTTTTCTGGAGCCAAACGTGCTAAGTCGGTGAATTTGAGATTCAAATTGCCATACTATACTCATTGGGGTCAAAGCTTGGTAGTTTGTGGCTCTGACTCCTTGGTTGGTTCGTGGAATGTGAAGAAGGGGTTGTTACTGAGTCCTGTTCACCAAGGAGATCAGCTTATATGGTGTGGAAGTATTGCGGTGTCTGATGGATTTGAATGTGAGTACAATTATTATGTTGTTGATGACAATAGAAACGTCTTGAGATGGGAGAAGGGAAACAGGAGAAAGGTGTTGTTACCTCAAGGATTACAGGGTGCGGAGGTCATCGAGCTCCGTGATCTTTGGCAGGTGCTCTCTTTTACATTATATTCACCAGTGCTTATTCTTCTTACTATCGTTTCAATTTCTTTGACGTTTCAGCTCGTTGAATTCTATAGGAGACTATTGTATGGCTTTGATTTAATTTGGTTATTGAACATTACGTAAGAATAAACTATGAGATAGTAAGAATAAATAAACTAAATAGAGGTCGGAACTTTTCACTCAAAGAAGCTTCTATCTTCCAAAAATTTTCCATTATTATTTCAATGAAGAAGGAAAACACCCCTGCCTGACACAAAATCATCTCCTTTGCGAATAAATTAACCGATGGCTCCACCTCCTAGCCTCTATTGAGTTGCAACCATGTGATACTGTGCTTCCTCTGATAATAGTGGTTAGGGGTTTCACCCTGTCAATGCTACATTACAAGTTAAGTATAAAACTTCATTTTCTTAGAAAAGTTCACCTTTCATATCCTCAACTAGGATGAATGGCACTTAGAGATGACGCAACAACAGTGGAGCTGTTATGTGGTGCTTGTATAAAAAAGAGTCATCAGGGATAGAGATTTCACATATTTCTATCTATTTGAGCAACGTCCTAAGCAGAACAAGAAAAGGGAAAAGACCAGTTGATTTTCTCTATCATTAAGGTTTCTTGTAAAAGTCAATTTCAAATTCCAAGAGATAAAAGAGCATGAGTCATGACATTGATGAAATGGAGGAAGTTTTAGCAGGAGTAACATTCTAATAACAAGGGAATCTGAGAGTCAGAGTAGGCCCCAATCTTCTGCAAAAAATGGATATTTTTCTCACTAGCCGAAGCAAAGGAGAACTTAATGAAGAACTGATTGATGGCTGTTCGTGGACTACACTTTGAGGGTCAGAGGGAACCTAAGAAGCATCCTAGTTGTTAAAGAAAACCTTGACCTACAAGCGAAAATCTTATACTGAAGGCAAAACACTCCCATGACTCTCTATCAACCACTTGTCTAGCAGGATCTGATTCTTATGGACCAAAGTATCTACACTGAATCCACCTTCGGTTGTTTATTGTAGCATGGTTTAGTACCAAATTTCCAATGAAGTTGATTTTAGGTAAGATGAGAGTCTGTTTGCCTTGGTGACTGAGAATTGTAACACTAAAGTAATGTTCTGGGCTTGGGGAATTGGAGTTACATTTCAACCTAGGCAGTTATTTATTTATTTATTTTTGGTAAAATTTCCATTTTTTATTAAAAAGATTCATTCATTTTGACTTGTCATTAACGTTCTATATAAAACTTGTTTCGGGAAATGTTTCATGCCAATGAGATGATTATTAATTATGCTTGGATTTGTGCATTTATTTTTTACATTTCAAAGACTTTGCAATACGTTGCCTTTCAGTTTTCTTCTGGTTACTCTCGTAATTTTTTTTCTGAAAATTTTGTACATATTGGTATTTAATAGATGAATTTTGGTCTCTGGAATCTACGAGCATTAGCCTGATTTTAAAGATCTCATCTATTCTTCTCTTTGATACATCAACTCAGACTGGTGCAGATGCGATTCCTTTCAAGAGTGCCTTTAAAGATGTCATCTTTGGAAGAAGTTCAACTTTGAGCATAGAAAGACCACTAGGAAATTTCGTTCATAGTTTGGATGAAGATGGTAAGTTTAATATTTTCTTTGTTATTTCGTGAATATCCTTCTTTAACATAATATGATTACTTCTGGGTAATCTTATCTTAACCAAGTTGTCGTTTTTTTTACTTGACTGAAGCAGACTCTGTTCTTGTCCATTTCAAAATTTGCTGTCCAAATATAGAAGAAGATACAACAGTAAATATATATTTCTCTACCCTGCTTTATTTCTTATCTGCAAATAGTTACTTCACACTGACTGGCGTTCAATTTCAATTCTAGTTATAATTTGTGAAATTTTACTAGTGCAGATCTATGTCATTGGTAGTTCTTCGAAATTAGGACAGTGGAAGGTACAGAATGGAATTAAACTTAGCCATGCGGGTGACTCAATCTGGCATGGAGACTGCATCTTACAATTCAGCGATTTCCCCTTAAAATATCCTTTTCTGTTGTTTTCTTTTACAATTGGAACAATTTCCTATGTTTATGTTTATTCCTTTCTTTCCCCTACCTATTCCTTAATAACAATTTCACGTACAAGTATTGTAAATATGGAAAAGCAGGAGTTATTTCTTCAGAATTTGGGCAAAACCGGGACCTTCTCCTTGATGCTTCAAATTTTCCACCAAGATATATTTTACTTTCAGATGGCATGTTGCGAGTAAGCTATTGTGAAGAAATTTCTTCTGAAGAATACTAGTTAGTTTTTTAGAAAATTAGTTCATAAATTTCTTGTACTCAGGATCTTCCTTGGCGTGGTTCTGGTGTTGCAATCCCCATGTTCTCTGTTAGATCAGACGATGATCTTGGAGTTGGCGAGTTTCTTGACTTGAAATTACTTGTTGACTGGGCTGTGGAGTCTGGTCTCCATTTAGTTCAACTTCTACCTGTTAATGATACATCCGTCCATGGAATGTGGTGGGACTCGTATCCTTACAGGTAGGA

mRNA sequence

AAGAAATTTGTATAAATCCCCATGAAAATCATCGAAGGTAAGATATTTTACAACTTAGATCATTCCATGTGCTTTTAAATTTTGATGCTTTCGTTCTCCTCCACATGGGTCCGAAGATTTTCCGGGAAAATTTCGTGGGGAAAAGTTTCTCAGAACTGTCCAAATGAATTTGTATAACAAGAACAGAAAGATTTCAATTCTACGGTCACTATTCACTTTTTCATTGAACGCCAAAATACAACTCGTTCGAACCTCTCTCTACTACTACAAGACATTCATCACCAAAATATCAAGCAACCCCATCAATCCCTCTCTTACTTTATATCTTCGAGAACGTGGTGATTACTGATTTCCCACTTCCCTACTATATTCTACACCAGCAAGGGAGATTCTCCTTACTGCATTTTCCAACCGAACGAATTCATCTCTTCAATTTCGTTCTTCCTATTTGAAAACTTGTTCTGGGTCTGTTTCAATTCTGAATAAGATGGTAAATTTGGGATTGTTTTCTGGAGCCAAACGTGCTAAGTCGGTGAATTTGAGATTCAAATTGCCATACTATACTCATTGGGGTCAAAGCTTGGTAGTTTGTGGCTCTGACTCCTTGGTTGGTTCGTGGAATGTGAAGAAGGGGTTGTTACTGAGTCCTGTTCACCAAGGAGATCAGCTTATATGGTGTGGAAGTATTGCGGTGTCTGATGGATTTGAATGTGAGTACAATTATTATGTTGTTGATGACAATAGAAACGTCTTGAGATGGGAGAAGGGAAACAGGAGAAAGGTGTTGTTACCTCAAGGATTACAGGGTGCGGAGGTCATCGAGCTCCGTGATCTTTGGCAGACTGGTGCAGATGCGATTCCTTTCAAGAGTGCCTTTAAAGATGTCATCTTTGGAAGAAGTTCAACTTTGAGCATAGAAAGACCACTAGGAAATTTCGTTCATAGTTTGGATGAAGATGACTCTGTTCTTGTCCATTTCAAAATTTGCTGTCCAAATATAGAAGAAGATACAACAATCTATGTCATTGGTAGTTCTTCGAAATTAGGACAGTGGAAGGTACAGAATGGAATTAAACTTAGCCATGCGGGTGACTCAATCTGGCATGGAGACTGCATCTTACAATTCAGCGATTTCCCCTTAAAGTACAAGTATTGTAAATATGGAAAAGCAGGAGTTATTTCTTCAGAATTTGGGCAAAACCGGGACCTTCTCCTTGATGCTTCAAATTTTCCACCAAGATATATTTTACTTTCAGATGGCATGTTGCGAGATCTTCCTTGGCGTGGTTCTGGTGTTGCAATCCCCATGTTCTCTGTTAGATCAGACGATGATCTTGGAGTTGGCGAGTTTCTTGACTTGAAATTACTTGTTGACTGGGCTGTGGAGTCTGGTCTCCATTTAGTTCAACTTCTACCTGTTAATGATACATCCGTCCATGGAATGTGGTGGGACTCGTATCCTTACAGGTAGGA

Coding sequence (CDS)

ATGGTAAATTTGGGATTGTTTTCTGGAGCCAAACGTGCTAAGTCGGTGAATTTGAGATTCAAATTGCCATACTATACTCATTGGGGTCAAAGCTTGGTAGTTTGTGGCTCTGACTCCTTGGTTGGTTCGTGGAATGTGAAGAAGGGGTTGTTACTGAGTCCTGTTCACCAAGGAGATCAGCTTATATGGTGTGGAAGTATTGCGGTGTCTGATGGATTTGAATGTGAGTACAATTATTATGTTGTTGATGACAATAGAAACGTCTTGAGATGGGAGAAGGGAAACAGGAGAAAGGTGTTGTTACCTCAAGGATTACAGGGTGCGGAGGTCATCGAGCTCCGTGATCTTTGGCAGACTGGTGCAGATGCGATTCCTTTCAAGAGTGCCTTTAAAGATGTCATCTTTGGAAGAAGTTCAACTTTGAGCATAGAAAGACCACTAGGAAATTTCGTTCATAGTTTGGATGAAGATGACTCTGTTCTTGTCCATTTCAAAATTTGCTGTCCAAATATAGAAGAAGATACAACAATCTATGTCATTGGTAGTTCTTCGAAATTAGGACAGTGGAAGGTACAGAATGGAATTAAACTTAGCCATGCGGGTGACTCAATCTGGCATGGAGACTGCATCTTACAATTCAGCGATTTCCCCTTAAAGTACAAGTATTGTAAATATGGAAAAGCAGGAGTTATTTCTTCAGAATTTGGGCAAAACCGGGACCTTCTCCTTGATGCTTCAAATTTTCCACCAAGATATATTTTACTTTCAGATGGCATGTTGCGAGATCTTCCTTGGCGTGGTTCTGGTGTTGCAATCCCCATGTTCTCTGTTAGATCAGACGATGATCTTGGAGTTGGCGAGTTTCTTGACTTGAAATTACTTGTTGACTGGGCTGTGGAGTCTGGTCTCCATTTAGTTCAACTTCTACCTGTTAATGATACATCCGTCCATGGAATGTGGTGGGACTCGTATCCTTACAGGTAG

Protein sequence

MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQLIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTGADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVIGSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRDLLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVESGLHLVQLLPVNDTSVHGMWWDSYPYR*
Homology
BLAST of CSPI04G18850 vs. ExPASy Swiss-Prot
Match: Q8RXD9 (4-alpha-glucanotransferase DPE2 OS=Arabidopsis thaliana OX=3702 GN=DPE2 PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 1.8e-120
Identity = 196/329 (59.57%), Postives = 253/329 (76.90%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKS---VNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQ 60
           M+NLG  S +    S   V++ F +PY+THWG+SL+VCGS   +GS NVKKGLLL P  Q
Sbjct: 1   MMNLGSLSLSTSKSSKPMVSISFWIPYFTHWGESLLVCGSAPGLGSGNVKKGLLLKPSQQ 60

Query: 61  GDQLIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLW 120
            DQLIW GS++V  GF  +Y YYVVDD+++VLR E G +RK+++P+ L G E + LRDLW
Sbjct: 61  DDQLIWSGSVSVPPGFSSDYCYYVVDDSKSVLRSEFGMKRKLVVPETLTGGESVHLRDLW 120

Query: 121 QTGADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTI 180
           Q+G  A+PF+SAFKDVIF  S  + +E+PLG F++  D+DDSV+V FKICCP+I E T++
Sbjct: 121 QSGDQALPFRSAFKDVIFHHSFDVKVEKPLGVFMNKSDQDDSVVVQFKICCPDIGEGTSV 180

Query: 181 YVIGSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQ 240
           YV+G+  KLG WKV+NG++L++  DSIW  DC++  +DFP+KY+YCK  K   I  E G 
Sbjct: 181 YVLGTPEKLGNWKVENGLRLNYVDDSIWEADCLIPKADFPIKYRYCKVQKEDSIGFESGG 240

Query: 241 NRDLLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDW 300
           NR+L L +      YI++SDG+ R +PWRG+GVA+PMFSVRS+DD+GVGEFLDLKLLVDW
Sbjct: 241 NRELSLHSIGSKQEYIVMSDGLFRAMPWRGAGVAVPMFSVRSEDDVGVGEFLDLKLLVDW 300

Query: 301 AVESGLHLVQLLPVNDTSVHGMWWDSYPY 327
           AV+SGLHLVQLLPVNDTSVH MWWDSYPY
Sbjct: 301 AVDSGLHLVQLLPVNDTSVHKMWWDSYPY 329

BLAST of CSPI04G18850 vs. ExPASy Swiss-Prot
Match: Q69Q02 (4-alpha-glucanotransferase DPE2 OS=Oryza sativa subsp. japonica OX=39947 GN=DPE2 PE=2 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 2.5e-117
Identity = 191/327 (58.41%), Postives = 252/327 (77.06%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           M NL   SG K   +V L FKLPYYT WGQSL++ GS+  +GSWNVK+GL LSPVHQG++
Sbjct: 1   MTNL---SGKKSLNTVTLVFKLPYYTQWGQSLLIAGSEPALGSWNVKQGLSLSPVHQGNE 60

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIW G ++V+ GF C+YNYYVVDDN+NVLR E G +RK++LP+G+Q  +V+E+RD WQ  
Sbjct: 61  LIWSGRVSVATGFTCQYNYYVVDDNKNVLRSESGEKRKLVLPEGVQDGDVVEIRDWWQDA 120

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI 180
           ++A+  +SAFK+VIF  S     E    +   SL+ +D ++V F + CP +   +T+ V 
Sbjct: 121 SEALFLRSAFKNVIFNGSENAKRELKTTSLNKSLEPED-IVVQFIVSCPRLGAGSTVVVT 180

Query: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240
           GS+ +LG+W+ Q+G+KL++ GDSIW  +C+L+ S+FP+KYKYCK  +AGV S EFG NR+
Sbjct: 181 GSNPQLGRWQTQDGLKLNYVGDSIWKANCLLRKSEFPIKYKYCKISEAGVSSLEFGPNRE 240

Query: 241 LLLDASN-FPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAV 300
             +D S+  P RY+LLSDG LR+ PWRG+GVA+P+FS+RS++DLGVGEFLDLKLLVDWAV
Sbjct: 241 ADVDLSSPKPSRYVLLSDGALRESPWRGAGVAVPIFSIRSNEDLGVGEFLDLKLLVDWAV 300

Query: 301 ESGLHLVQLLPVNDTSVHGMWWDSYPY 327
            SG HLVQLLP+NDTSVHGMWWDSYPY
Sbjct: 301 NSGFHLVQLLPINDTSVHGMWWDSYPY 323

BLAST of CSPI04G18850 vs. ExPASy Swiss-Prot
Match: O84089 (4-alpha-glucanotransferase OS=Chlamydia trachomatis (strain D/UW-3/Cx) OX=272561 GN=malQ PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 9.4e-08
Identity = 21/46 (45.65%), Postives = 34/46 (73.91%), Query Frame = 0

Query: 269 GVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVESGLHLVQLLPVNDT 315
           GV +P+FS+ + +  G+GEFLDL  ++DW +  G  ++Q+LP+NDT
Sbjct: 30  GVCVPLFSLYTQESCGIGEFLDLIPMIDWCISCGFQILQILPINDT 75

BLAST of CSPI04G18850 vs. ExPASy Swiss-Prot
Match: Q9PKU9 (4-alpha-glucanotransferase OS=Chlamydia muridarum (strain MoPn / Nigg) OX=243161 GN=malQ PE=3 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.7e-07
Identity = 20/46 (43.48%), Postives = 33/46 (71.74%), Query Frame = 0

Query: 269 GVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVESGLHLVQLLPVNDT 315
           G+ +P+FS+ + +  G+GEFLDL  ++DW    G  ++Q+LP+NDT
Sbjct: 30  GICVPLFSIHTQNSCGIGEFLDLIPMIDWCTLCGFQILQILPINDT 75

BLAST of CSPI04G18850 vs. ExPASy Swiss-Prot
Match: Q9Z8L2 (4-alpha-glucanotransferase OS=Chlamydia pneumoniae OX=83558 GN=malQ PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.6e-07
Identity = 24/58 (41.38%), Postives = 37/58 (63.79%), Query Frame = 0

Query: 269 GVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVESGLHLVQLLPVNDTSVHGMWWDSYPY 327
           G+ +P+FS+ + +  G+GEFLDL  L+ W  + G  ++QLLP+NDT       D+ PY
Sbjct: 26  GIYLPLFSIHTKNSCGIGEFLDLIPLISWCQKQGFSVIQLLPLNDTGE-----DTSPY 78

BLAST of CSPI04G18850 vs. ExPASy TrEMBL
Match: A0A0A0L0U4 (Amylomaltase OS=Cucumis sativus OX=3659 GN=Csa_4G420150 PE=3 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 1.8e-195
Identity = 324/326 (99.39%), Postives = 325/326 (99.69%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           MVNLGLFSGAKRAKSVN+RFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ
Sbjct: 42  MVNLGLFSGAKRAKSVNVRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 101

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG
Sbjct: 102 LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 161

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI 180
            DAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI
Sbjct: 162 GDAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI 221

Query: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240
           GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD
Sbjct: 222 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 281

Query: 241 LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300
           LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE
Sbjct: 282 LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 341

Query: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 327
           SGLHLVQLLPVNDTSVHGMWWDSYPY
Sbjct: 342 SGLHLVQLLPVNDTSVHGMWWDSYPY 367

BLAST of CSPI04G18850 vs. ExPASy TrEMBL
Match: A0A1S4E1F0 (Amylomaltase OS=Cucumis melo OX=3656 GN=LOC103495851 PE=3 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 4.9e-193
Identity = 319/326 (97.85%), Postives = 323/326 (99.08%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           MVNLGLFSGAKRAKSVN+RF+LPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ
Sbjct: 1   MVNLGLFSGAKRAKSVNVRFRLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIWCGSIAVS+GFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQG EVIELRDLWQTG
Sbjct: 61  LIWCGSIAVSNGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGGEVIELRDLWQTG 120

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI 180
           ADAIPFKSAFKDVIFGRSSTLSIERPLGNF+HSLDEDDSVLVHFKICCPNIEEDT IYVI
Sbjct: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFIHSLDEDDSVLVHFKICCPNIEEDTRIYVI 180

Query: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240
           GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD
Sbjct: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240

Query: 241 LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300
           LLLDASNFPPRYI LSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE
Sbjct: 241 LLLDASNFPPRYISLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300

Query: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 327
           SGLHLVQLLPVNDTSVHGMWWDSYPY
Sbjct: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 326

BLAST of CSPI04G18850 vs. ExPASy TrEMBL
Match: A0A1S3C2V9 (Amylomaltase OS=Cucumis melo OX=3656 GN=LOC103495851 PE=3 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 4.9e-193
Identity = 319/326 (97.85%), Postives = 323/326 (99.08%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           MVNLGLFSGAKRAKSVN+RF+LPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ
Sbjct: 1   MVNLGLFSGAKRAKSVNVRFRLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIWCGSIAVS+GFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQG EVIELRDLWQTG
Sbjct: 61  LIWCGSIAVSNGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGGEVIELRDLWQTG 120

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI 180
           ADAIPFKSAFKDVIFGRSSTLSIERPLGNF+HSLDEDDSVLVHFKICCPNIEEDT IYVI
Sbjct: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFIHSLDEDDSVLVHFKICCPNIEEDTRIYVI 180

Query: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240
           GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD
Sbjct: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240

Query: 241 LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300
           LLLDASNFPPRYI LSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE
Sbjct: 241 LLLDASNFPPRYISLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300

Query: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 327
           SGLHLVQLLPVNDTSVHGMWWDSYPY
Sbjct: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 326

BLAST of CSPI04G18850 vs. ExPASy TrEMBL
Match: A0A6J1HV80 (Amylomaltase OS=Cucurbita maxima OX=3661 GN=LOC111467152 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 6.9e-179
Identity = 294/327 (89.91%), Postives = 315/327 (96.33%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           MVNLGLFSGAKRAKSVN+RF+LPYYT WGQSLVVCGS+ L+GSWNVKKGLLLSP+HQGDQ
Sbjct: 1   MVNLGLFSGAKRAKSVNVRFRLPYYTQWGQSLVVCGSEPLLGSWNVKKGLLLSPIHQGDQ 60

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIW GSIAVS+GF CEYNYYVVDDNR+VLRWEKGNRRK+ LPQGLQG EV+ELRDLWQTG
Sbjct: 61  LIWYGSIAVSNGFGCEYNYYVVDDNRDVLRWEKGNRRKLFLPQGLQGGEVVELRDLWQTG 120

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVH-SLDEDDSVLVHFKICCPNIEEDTTIYV 180
           ADAIPFK AFKDV+FGRSSTLSIERPLG F+H +L+EDDSVLV FKICCPNIEEDTT+YV
Sbjct: 121 ADAIPFKLAFKDVVFGRSSTLSIERPLGKFIHNNLNEDDSVLVDFKICCPNIEEDTTVYV 180

Query: 181 IGSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNR 240
           IGSSSKLGQWKVQNGIKLS+AGDSIWHGDCILQ++DFPLKYKYCKYGK GVISSEFGQNR
Sbjct: 181 IGSSSKLGQWKVQNGIKLSYAGDSIWHGDCILQYTDFPLKYKYCKYGKDGVISSEFGQNR 240

Query: 241 DLLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAV 300
           DLLLDASN PPR+I+LSDGMLRD+PWRGSGVAIPMFSVRS+DDLGVGEFLDLKLLVDWAV
Sbjct: 241 DLLLDASNIPPRHIVLSDGMLRDVPWRGSGVAIPMFSVRSEDDLGVGEFLDLKLLVDWAV 300

Query: 301 ESGLHLVQLLPVNDTSVHGMWWDSYPY 327
           ESGLHLVQLLPVNDTSVHGMWWDSYPY
Sbjct: 301 ESGLHLVQLLPVNDTSVHGMWWDSYPY 327

BLAST of CSPI04G18850 vs. ExPASy TrEMBL
Match: A0A6J1GFH3 (Amylomaltase OS=Cucurbita moschata OX=3662 GN=LOC111453446 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.6e-178
Identity = 293/327 (89.60%), Postives = 313/327 (95.72%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           MVNLGLFSGAKRAKSV++RF+LPYYT WGQSLVVCGS+ L+GSWNVKKGLLLSP+HQGDQ
Sbjct: 1   MVNLGLFSGAKRAKSVSVRFRLPYYTQWGQSLVVCGSEPLLGSWNVKKGLLLSPIHQGDQ 60

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIW GSIAVSDGF CEYNYYVVDDNR VLRWEKGNRRK+ LPQGLQG EV+ELRDLWQTG
Sbjct: 61  LIWYGSIAVSDGFGCEYNYYVVDDNREVLRWEKGNRRKLFLPQGLQGGEVVELRDLWQTG 120

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVH-SLDEDDSVLVHFKICCPNIEEDTTIYV 180
            DAIPFK AFKDV+FGRSSTLSIERPLG F+H +L+EDDSVLV FKICCPNIEEDTT+YV
Sbjct: 121 VDAIPFKLAFKDVVFGRSSTLSIERPLGKFIHNNLNEDDSVLVDFKICCPNIEEDTTVYV 180

Query: 181 IGSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNR 240
           IGSSSKLGQWKVQNGIKLS+AGDSIWHGDCILQ++DFPLKYKYCKYGK GVISSEFGQNR
Sbjct: 181 IGSSSKLGQWKVQNGIKLSYAGDSIWHGDCILQYTDFPLKYKYCKYGKDGVISSEFGQNR 240

Query: 241 DLLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAV 300
           DLLLDASN PPR+I+LSDGMLRD+PWRGSGVAIPMFSVRS+DDLGVGEFLDLKLLVDWAV
Sbjct: 241 DLLLDASNIPPRHIVLSDGMLRDVPWRGSGVAIPMFSVRSEDDLGVGEFLDLKLLVDWAV 300

Query: 301 ESGLHLVQLLPVNDTSVHGMWWDSYPY 327
           ESGLHLVQLLPVNDTSVHGMWWDSYPY
Sbjct: 301 ESGLHLVQLLPVNDTSVHGMWWDSYPY 327

BLAST of CSPI04G18850 vs. NCBI nr
Match: XP_004144319.1 (4-alpha-glucanotransferase DPE2 [Cucumis sativus])

HSP 1 Score: 691.4 bits (1783), Expect = 3.7e-195
Identity = 324/326 (99.39%), Postives = 325/326 (99.69%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           MVNLGLFSGAKRAKSVN+RFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ
Sbjct: 1   MVNLGLFSGAKRAKSVNVRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG
Sbjct: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI 180
            DAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI
Sbjct: 121 GDAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI 180

Query: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240
           GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD
Sbjct: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240

Query: 241 LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300
           LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE
Sbjct: 241 LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300

Query: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 327
           SGLHLVQLLPVNDTSVHGMWWDSYPY
Sbjct: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 326

BLAST of CSPI04G18850 vs. NCBI nr
Match: XP_016901815.1 (PREDICTED: 4-alpha-glucanotransferase DPE2 isoform X2 [Cucumis melo])

HSP 1 Score: 683.3 bits (1762), Expect = 1.0e-192
Identity = 319/326 (97.85%), Postives = 323/326 (99.08%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           MVNLGLFSGAKRAKSVN+RF+LPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ
Sbjct: 1   MVNLGLFSGAKRAKSVNVRFRLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIWCGSIAVS+GFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQG EVIELRDLWQTG
Sbjct: 61  LIWCGSIAVSNGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGGEVIELRDLWQTG 120

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI 180
           ADAIPFKSAFKDVIFGRSSTLSIERPLGNF+HSLDEDDSVLVHFKICCPNIEEDT IYVI
Sbjct: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFIHSLDEDDSVLVHFKICCPNIEEDTRIYVI 180

Query: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240
           GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD
Sbjct: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240

Query: 241 LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300
           LLLDASNFPPRYI LSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE
Sbjct: 241 LLLDASNFPPRYISLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300

Query: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 327
           SGLHLVQLLPVNDTSVHGMWWDSYPY
Sbjct: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 326

BLAST of CSPI04G18850 vs. NCBI nr
Match: XP_008455750.1 (PREDICTED: 4-alpha-glucanotransferase DPE2 isoform X1 [Cucumis melo])

HSP 1 Score: 683.3 bits (1762), Expect = 1.0e-192
Identity = 319/326 (97.85%), Postives = 323/326 (99.08%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           MVNLGLFSGAKRAKSVN+RF+LPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ
Sbjct: 1   MVNLGLFSGAKRAKSVNVRFRLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIWCGSIAVS+GFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQG EVIELRDLWQTG
Sbjct: 61  LIWCGSIAVSNGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGGEVIELRDLWQTG 120

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI 180
           ADAIPFKSAFKDVIFGRSSTLSIERPLGNF+HSLDEDDSVLVHFKICCPNIEEDT IYVI
Sbjct: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFIHSLDEDDSVLVHFKICCPNIEEDTRIYVI 180

Query: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240
           GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD
Sbjct: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240

Query: 241 LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300
           LLLDASNFPPRYI LSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE
Sbjct: 241 LLLDASNFPPRYISLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300

Query: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 327
           SGLHLVQLLPVNDTSVHGMWWDSYPY
Sbjct: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 326

BLAST of CSPI04G18850 vs. NCBI nr
Match: XP_038882055.1 (4-alpha-glucanotransferase DPE2 [Benincasa hispida])

HSP 1 Score: 661.4 bits (1705), Expect = 4.1e-186
Identity = 303/326 (92.94%), Postives = 320/326 (98.16%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           MVNLGLFSGA+RAKS+N+RF+LPYYTHWGQSLV+CGSD L+GSWNVKKGLLLSPVHQGDQ
Sbjct: 1   MVNLGLFSGARRAKSMNVRFRLPYYTHWGQSLVICGSDPLLGSWNVKKGLLLSPVHQGDQ 60

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQG EVIELRDLWQTG
Sbjct: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGGEVIELRDLWQTG 120

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTIYVI 180
           ADAIPFKSAFKDVIFGRSSTLS+ERPLGNF+H++DEDDSVLVHFKICCPNIEEDTTIYVI
Sbjct: 121 ADAIPFKSAFKDVIFGRSSTLSLERPLGNFIHTVDEDDSVLVHFKICCPNIEEDTTIYVI 180

Query: 181 GSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNRD 240
           GSS KLGQWKVQNGI+LS+AGDSIWHGDCILQ+S FPLKYKYCKYGKAGV+SSEFGQNRD
Sbjct: 181 GSSPKLGQWKVQNGIRLSYAGDSIWHGDCILQYSHFPLKYKYCKYGKAGVVSSEFGQNRD 240

Query: 241 LLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAVE 300
           LLLDASN PPR+ILLSDGMLRD+PWRGSGVAIPMFSVRS+DDLGVGEFLDLKLLVDWAVE
Sbjct: 241 LLLDASNVPPRHILLSDGMLRDVPWRGSGVAIPMFSVRSEDDLGVGEFLDLKLLVDWAVE 300

Query: 301 SGLHLVQLLPVNDTSVHGMWWDSYPY 327
           SG HLVQLLPVNDTSVHGMWWDSYPY
Sbjct: 301 SGHHLVQLLPVNDTSVHGMWWDSYPY 326

BLAST of CSPI04G18850 vs. NCBI nr
Match: XP_022967705.1 (4-alpha-glucanotransferase DPE2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 636.3 bits (1640), Expect = 1.4e-178
Identity = 294/327 (89.91%), Postives = 315/327 (96.33%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKSVNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQGDQ 60
           MVNLGLFSGAKRAKSVN+RF+LPYYT WGQSLVVCGS+ L+GSWNVKKGLLLSP+HQGDQ
Sbjct: 1   MVNLGLFSGAKRAKSVNVRFRLPYYTQWGQSLVVCGSEPLLGSWNVKKGLLLSPIHQGDQ 60

Query: 61  LIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLWQTG 120
           LIW GSIAVS+GF CEYNYYVVDDNR+VLRWEKGNRRK+ LPQGLQG EV+ELRDLWQTG
Sbjct: 61  LIWYGSIAVSNGFGCEYNYYVVDDNRDVLRWEKGNRRKLFLPQGLQGGEVVELRDLWQTG 120

Query: 121 ADAIPFKSAFKDVIFGRSSTLSIERPLGNFVH-SLDEDDSVLVHFKICCPNIEEDTTIYV 180
           ADAIPFK AFKDV+FGRSSTLSIERPLG F+H +L+EDDSVLV FKICCPNIEEDTT+YV
Sbjct: 121 ADAIPFKLAFKDVVFGRSSTLSIERPLGKFIHNNLNEDDSVLVDFKICCPNIEEDTTVYV 180

Query: 181 IGSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQNR 240
           IGSSSKLGQWKVQNGIKLS+AGDSIWHGDCILQ++DFPLKYKYCKYGK GVISSEFGQNR
Sbjct: 181 IGSSSKLGQWKVQNGIKLSYAGDSIWHGDCILQYTDFPLKYKYCKYGKDGVISSEFGQNR 240

Query: 241 DLLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDWAV 300
           DLLLDASN PPR+I+LSDGMLRD+PWRGSGVAIPMFSVRS+DDLGVGEFLDLKLLVDWAV
Sbjct: 241 DLLLDASNIPPRHIVLSDGMLRDVPWRGSGVAIPMFSVRSEDDLGVGEFLDLKLLVDWAV 300

Query: 301 ESGLHLVQLLPVNDTSVHGMWWDSYPY 327
           ESGLHLVQLLPVNDTSVHGMWWDSYPY
Sbjct: 301 ESGLHLVQLLPVNDTSVHGMWWDSYPY 327

BLAST of CSPI04G18850 vs. TAIR 10
Match: AT2G40840.1 (disproportionating enzyme 2 )

HSP 1 Score: 433.7 bits (1114), Expect = 1.3e-121
Identity = 196/329 (59.57%), Postives = 253/329 (76.90%), Query Frame = 0

Query: 1   MVNLGLFSGAKRAKS---VNLRFKLPYYTHWGQSLVVCGSDSLVGSWNVKKGLLLSPVHQ 60
           M+NLG  S +    S   V++ F +PY+THWG+SL+VCGS   +GS NVKKGLLL P  Q
Sbjct: 1   MMNLGSLSLSTSKSSKPMVSISFWIPYFTHWGESLLVCGSAPGLGSGNVKKGLLLKPSQQ 60

Query: 61  GDQLIWCGSIAVSDGFECEYNYYVVDDNRNVLRWEKGNRRKVLLPQGLQGAEVIELRDLW 120
            DQLIW GS++V  GF  +Y YYVVDD+++VLR E G +RK+++P+ L G E + LRDLW
Sbjct: 61  DDQLIWSGSVSVPPGFSSDYCYYVVDDSKSVLRSEFGMKRKLVVPETLTGGESVHLRDLW 120

Query: 121 QTGADAIPFKSAFKDVIFGRSSTLSIERPLGNFVHSLDEDDSVLVHFKICCPNIEEDTTI 180
           Q+G  A+PF+SAFKDVIF  S  + +E+PLG F++  D+DDSV+V FKICCP+I E T++
Sbjct: 121 QSGDQALPFRSAFKDVIFHHSFDVKVEKPLGVFMNKSDQDDSVVVQFKICCPDIGEGTSV 180

Query: 181 YVIGSSSKLGQWKVQNGIKLSHAGDSIWHGDCILQFSDFPLKYKYCKYGKAGVISSEFGQ 240
           YV+G+  KLG WKV+NG++L++  DSIW  DC++  +DFP+KY+YCK  K   I  E G 
Sbjct: 181 YVLGTPEKLGNWKVENGLRLNYVDDSIWEADCLIPKADFPIKYRYCKVQKEDSIGFESGG 240

Query: 241 NRDLLLDASNFPPRYILLSDGMLRDLPWRGSGVAIPMFSVRSDDDLGVGEFLDLKLLVDW 300
           NR+L L +      YI++SDG+ R +PWRG+GVA+PMFSVRS+DD+GVGEFLDLKLLVDW
Sbjct: 241 NRELSLHSIGSKQEYIVMSDGLFRAMPWRGAGVAVPMFSVRSEDDVGVGEFLDLKLLVDW 300

Query: 301 AVESGLHLVQLLPVNDTSVHGMWWDSYPY 327
           AV+SGLHLVQLLPVNDTSVH MWWDSYPY
Sbjct: 301 AVDSGLHLVQLLPVNDTSVHKMWWDSYPY 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RXD91.8e-12059.574-alpha-glucanotransferase DPE2 OS=Arabidopsis thaliana OX=3702 GN=DPE2 PE=1 SV=... [more]
Q69Q022.5e-11758.414-alpha-glucanotransferase DPE2 OS=Oryza sativa subsp. japonica OX=39947 GN=DPE2... [more]
O840899.4e-0845.654-alpha-glucanotransferase OS=Chlamydia trachomatis (strain D/UW-3/Cx) OX=272561... [more]
Q9PKU92.7e-0743.484-alpha-glucanotransferase OS=Chlamydia muridarum (strain MoPn / Nigg) OX=243161... [more]
Q9Z8L23.6e-0741.384-alpha-glucanotransferase OS=Chlamydia pneumoniae OX=83558 GN=malQ PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L0U41.8e-19599.39Amylomaltase OS=Cucumis sativus OX=3659 GN=Csa_4G420150 PE=3 SV=1[more]
A0A1S4E1F04.9e-19397.85Amylomaltase OS=Cucumis melo OX=3656 GN=LOC103495851 PE=3 SV=1[more]
A0A1S3C2V94.9e-19397.85Amylomaltase OS=Cucumis melo OX=3656 GN=LOC103495851 PE=3 SV=1[more]
A0A6J1HV806.9e-17989.91Amylomaltase OS=Cucurbita maxima OX=3661 GN=LOC111467152 PE=3 SV=1[more]
A0A6J1GFH32.6e-17889.60Amylomaltase OS=Cucurbita moschata OX=3662 GN=LOC111453446 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004144319.13.7e-19599.394-alpha-glucanotransferase DPE2 [Cucumis sativus][more]
XP_016901815.11.0e-19297.85PREDICTED: 4-alpha-glucanotransferase DPE2 isoform X2 [Cucumis melo][more]
XP_008455750.11.0e-19297.85PREDICTED: 4-alpha-glucanotransferase DPE2 isoform X1 [Cucumis melo][more]
XP_038882055.14.1e-18692.944-alpha-glucanotransferase DPE2 [Benincasa hispida][more]
XP_022967705.11.4e-17889.914-alpha-glucanotransferase DPE2 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G40840.11.3e-12159.57disproportionating enzyme 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002044Carbohydrate binding module family 20SMARTSM01065CBM_20_2coord: 159..253
e-value: 1.0E-12
score: 58.3
coord: 15..111
e-value: 2.1E-15
score: 67.2
IPR002044Carbohydrate binding module family 20PFAMPF00686CBM_20coord: 160..244
e-value: 8.7E-12
score: 44.8
coord: 16..108
e-value: 2.9E-19
score: 68.8
IPR002044Carbohydrate binding module family 20PROSITEPS51166CBM20coord: 10..119
score: 18.40727
IPR002044Carbohydrate binding module family 20PROSITEPS51166CBM20coord: 154..267
score: 15.94089
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 8..119
e-value: 4.7E-21
score: 76.5
coord: 151..249
e-value: 4.6E-14
score: 54.0
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 264..327
e-value: 8.8E-13
score: 49.7
NoneNo IPR availablePANTHERPTHR32518FAMILY NOT NAMEDcoord: 11..327
IPR003385Glycoside hydrolase, family 77PFAMPF02446Glyco_hydro_77coord: 272..326
e-value: 1.2E-11
score: 44.1
IPR034840DPE2, CBM20 domain 1CDDcd05815CBM20_DPE2_repeat1coord: 17..117
e-value: 3.89653E-57
score: 178.407
IPR034841DPE2, CBM20 domain 2CDDcd05816CBM20_DPE2_repeat2coord: 162..258
e-value: 3.63666E-34
score: 118.968
IPR013784Carbohydrate-binding-like foldSUPERFAMILY49452Starch-binding domain-likecoord: 15..110
IPR013784Carbohydrate-binding-like foldSUPERFAMILY49452Starch-binding domain-likecoord: 157..247
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 265..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G18850.1CSPI04G18850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005977 glycogen metabolic process
biological_process GO:0006508 proteolysis
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004134 4-alpha-glucanotransferase activity
molecular_function GO:0102500 beta-maltose 4-alpha-glucanotransferase activity
molecular_function GO:0004175 endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity
molecular_function GO:2001070 starch binding
molecular_function GO:0030246 carbohydrate binding